scholarly journals Effects of castration and testosterone replacement on peritoneal histamine concentration and lung histamine concentration in pubertal male rats

2000 ◽  
Vol 167 (1) ◽  
pp. 71-75 ◽  
Author(s):  
AP Lima ◽  
LO Lunardi ◽  
E AA Rosa Silva
Keyword(s):  
Mast Cells ◽  
Significant Variation ◽  
Male Rats ◽  
Testosterone Replacement ◽  
Control Group ◽  
Biological Factors ◽  
Histamine Concentration ◽  
Wistar Strain ◽  
First Time ◽  
Different Tissues

Mast cells, which are the main source of histamine, are significantly affected by sex steroids. The present study was undertaken to determine the effects of bilateral castration and testosterone replacement on peritoneal histamine concentration and lung histamine concentration in pubertal male rats (Wistar strain). Three groups of animals were used in this study: (1) untreated castrated animals, (2) castrated animals subjected to androgen replacement by injection of propionate of testosterone, and (3) intact males as a control group. Castration alone produced a dramatic reduction in peritoneal histamine concentration. In addition, androgen replacement was effective in restoring the histamine concentration to the normal value detected in the control males (P<0.05, Kruskal-Wallis test). On the other hand, there was no significant variation in the lung histamine concentration between control males, untreated castrated males and castrated males that received androgen replacement (P<0.05, Kruskal-Wallis test). These results demonstrate for the first time that castration markedly reduces the peritoneum histamine concentration in pubertal male rats, and testosterone replacement prevents the decrease. Further, these procedures do not affect lung histamine concentration, demonstrating that mast cells from different tissues may respond differently to the same biological factors.

scholarly journals Ulcer Protective and Ulcer Healing Activities of Aqueous and Methanolic Extracts of Leaves of Rumex Bequaertii De Wild (Polygonaceae) in Rats

2015 ◽  
Vol 6 (2) ◽  
pp. 61
Author(s):  
Gilbert Ateufack ◽  
Breuil R. Dongmo Feudjio ◽  
William Nana Yousseu ◽  
Albert D. Atsamo ◽  
Albert Kamanyi
Keyword(s):  
Acetic Acid ◽  
Significant Variation ◽  
Healing Process ◽  
Negative Control ◽  
Chronic Ulcer ◽  
Male Rats ◽  
Control Group ◽  
Traditional Use ◽  
Mucus Production ◽  
Methanolic Extracts

The aqueous and methanolic extracts of Rumex bequaertii leaves were investigated for their ability to prevent and treated ulceration of the gastric mucosa in animal models.For this, adult male rats three to four months old and weighing on average 180 g were used for both tests. The extracts were tested orally at doses of 125, 250 and 500 mg/kg, on ulcerations experimentally induced by HCl/ethanol and pylorus ligation (acute ulcer) and acetic acid (chronic ulcer).These extracts showed protective and healing properties on ulcer induced models. A dose of 500 mg/kg of the two extracts inhibited ulceration induced by HCl/ethanol by 98.86 and 91.59 % respectively. With pyloric ligation, no significant variation of the volume of gastric juices, pH and gastric acidity was observed compared to the negative control rats. The aqueous extract alone at different doses presented a significant reduction of the ulceration surface resulting from chronic ulcer induced with acetic acid compared to the negative control group that received distilled water. For all three models of gastric ulcer induction used, the mass of mucus significantly increased in the groups that received the extracts. There was no significant variation in plasmatic nitric oxide (NO) concentrationand gastric supernatant of rats treated with both extracts compared to both control groups. These results reveal that the healing process of the extracts is not achieved through the NO pathway. This process could be due to increase in mucus production and thus supports its traditional use of the plant

scholarly journals UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 572
Author(s):  
Asriyani Samiun ◽  
Edwin De Queljoe ◽  
Irma Antasionasti
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Male Rats ◽  
Test Material ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain ◽  
One Way Anova ◽  
Vernonia Cinerea ◽  
Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit  (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit  setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

scholarly journals JUS SEMANGKA MENURUNKAN NEUTROFIL TIKUS JANTAN GALUR WISTAR YANG TERPAPAR ASAP ROKOK

2018 ◽  
Vol 11 (2) ◽  
pp. 166
Author(s):  
Rizky Prihandari ◽  
Lailatul Muniroh
Keyword(s):  
Cigarette Smoke ◽  
Hazardous Substances ◽  
Male Rats ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Post Test ◽  
Experimental Laboratory

Cigarette smoke contains many hazardous substances and free radical which affect respiratory system. Watermelon “Sugar Baby” juice contain lycopene as antiinfl ammation. The aim of this research was to analyze the effect of watermelon as antiinfl ammation toward neutrophils count in wistar strain male rats exposed by  cigarette smoke. This study was an experimental laboratory research with pre and post test control group design. Sample consisted of 24 wistar strain male rats with ±150-230 grams weight and 3 months old which were divided into four groups. Control group was given placebo (aquadest) and treatment groups were given w atermelon juice with terraced quantity (1.5 ml, 2.5 ml, and 3.5 ml). Every group was given two sticks of cigarette smoked per day during two weeks then smoked with treatment during one week. Neutrophil from peripheral smear was observed by manual method and statistically analyzed using Anova Mixed Design and continued with One Way Anova. The result showed signifi cant increased of  neutrophil at second observation on control group (p=0.002) and treatment groups (p=0.000). Signifi cance decreased also found in the treatment groups’s at third observation p=0.000. Signifi cant between each group p= 0.017. There was signifi cant different between control and treatment groups but there was no signifi cant different between each treatment groups. This study concluded that there was decreased of  neutrophil in wistar strain male rats which were given w atermelon juice 1.5 ml, 2.5 ml, and 3.5 ml during infl ammation. 3.5 ml w atermelon juice which were given showed better result as antiinfl ammation..

scholarly journals THE EFFECTIVENESS OF CORIANDER (Coriandrum sativum L.) EXTRACTS ON REDUCING THE BLOOD SUGAR LEVELS OF ALLOXAN INDUCED WISTAR MALE RATS (Rattus norvegicus L.)

2020 ◽  
Vol 6 (1) ◽  
pp. 36-44
Author(s):  
Kalvin Dersing
Keyword(s):  
Blood Sugar ◽  
Rattus Norvegicus ◽  
Water Extract ◽  
Coriandrum Sativum ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Wistar Strain ◽  
Sugar Levels

AbstractBackground : Diabetes mellitus is the most common metabolic syndrome in the whole world with an incidence of 1-8%. This disease arises when insufficient insulin is produced or insulin cannot function properly.The ability of coriander juice water extract is expected to reduce blood glucose levels because the action is similar to insulin and can stimulate insulin release. Purpose : Knowing effectiveness coriander extract (Coriandrum sativum L.)to decline blood sugar levels in male white rats (Rattus norvegicus L.) wistar strain induced by alloxan. Method : This is a pure experimental study (true-experiment) using a pre and post test design with control group design using subjects namely white rats (Rattus norvegicus L.) male Wistar strain aged 10-12 weeks with a weight of 150-200 grams. By looking at changes in blood sugar levels during administration of alloxan and administration of coriander extract. Results : There were significant changes in some groups induced alloxan (p <0.05), but the effect of coriander extract was not very significant (p> 0.05). Conclusion : Coriander extract can control blood sugar levels to be stable or normal, but it takes longer and more research.  Keywords : coriander extract, blood sugar levels, alloxan

scholarly journals EXAMINATION OF GLUCOSES CONCENTRATION IN CHRONIC SEPSIS MODEL IN RATS

2009 ◽  
Vol 2 (1) ◽  
pp. 39-44
Author(s):  
Dragica Stojanović ◽  
Ružica Ašanin ◽  
Milica Ninković ◽  
Živorad Malićević ◽  
Igor Stojanov
Keyword(s):  
Cecal Ligation ◽  
Male Rats ◽  
Control Group ◽  
Clinical Form ◽  
Sepsis Model ◽  
Wistar Strain ◽  
Pure Bacterial Culture ◽  
Mixed Bacteria ◽  
Bacteria Culture ◽  
And Staphylococcus Aureus

We induced clinical form of sepsis in rats by cecal ligation and perforation (CLP) with mixed and pure culture of bacteria, with the aim to monitor glucose concentration in sepsis. The trial was carried out on 104 male rats, Wistar strain, body mass 190 to 240 g. The rats were divided in four groups consisting of 28 animals, and a control group of 20 animals. The period of observations were 12, 24, 72 and 120 hours after surgical intervention. In the examined sepsis model, a significant hypoglycemic activity was noticed in early sepsis (12 and 24 hours), but in the group with mixed bacteria culture only after 72 hours. Hyperglycemia was registered in late sepsis (72 hours) in the group of rats where sepsis was caused by pure bacterial culture of Escherichia coli and Staphylococcus aureus, without statistic significance compared to the findings in the control group.

TRIIODOTHYRONINE METABOLISM IN DIABETIC RATS

1978 ◽  
Vol 89 (2) ◽  
pp. 323-328 ◽  
Author(s):  
R. Boado ◽  
T. J. Brown ◽  
N. R. Bromage ◽  
A. J. Matty ◽  
A. A. Zaninovich
Keyword(s):  
Diabetic Rats ◽  
Cell Uptake ◽  
Male Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Straight Line ◽  
Induced Changes ◽  
Binding Avidity ◽  
Kinetics Of ◽  
Uptake Method

ABSTRACT Triiodothyronine (T3) metabolism was assessed in untreated and in insulin-treated diabetic rats and the results compared with a corresponding control group. Male rats of the Wistar strain were made diabetic by the intraperitoneal injection of alloxan in a dose of 17.5 mg/100 g body weight and thereafter maintained normoglycaemic for a week and until 24 h before initiation of the study. The average glycaemia in control and in insulin-treated animals was 135 mg/100 ml, whereas in the untreated diabetic rats it was 455 mg/100 ml. Each animal was injected a tracer dose of [125I]T3 containing 2 μCi of 125I and 15 ng of T3. Heparinized blood samples were obtained by cardiac puncture at 5, 18 and 24 h following the injection of the tracer. The excretion of inorganic 125I in the 24-h collections of urine was also determined. The kinetics of [125I]T3 were studied by the extrapolation method using the straight line of plasma disappearance between 5 and 24 h. It was found that the untreated diabetic rats had a significant slowing of plasma disappearance of labelled T3. The decrease in fractional hormone turnover was balanced by a slight expansion of the distribution space, which presumably led to a larger hormone pool and a normal degradation rate of T3. These events seemed substantiated by the normal excretion of 125I in the urine observed in untreated animals. A parallel study of plasma hormone binding using a red cell uptake method showed a significant reduction of binding avidity for T3 by plasma proteins in the untreated diabetic animals. The administration of insulin restored plasma T3 kinetics and binding avidity to normal, as observed in control rats. It was concluded that untreated diabetes mellitus induced changes in T3 kinetics and in T3 serum binding without altering hormone deiodination, parameter which reflects tissue utilization of T3.

Grape Seed and Physalis Extracts as Anti- Inflammatory Agents in Cetuximab Induced Tongue Mucositis in Rats

2020 ◽  
Vol 20 (3) ◽  
pp. 173
Author(s):  
Heba A. Saleh ◽  
Manar A. Abdul Aziz ◽  
Mona E. Wali
Keyword(s):  
Mast Cells ◽  
Cancer Therapy ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Male Rats ◽  
Control Group ◽  
Tissue Preparation ◽  
Group I ◽  
Area Percent

<strong>Background:</strong> Oral mucositis induced by cancer therapy alters cancer patient’s life. Nutraceuticals these days is for their various activities which could neutralize the toxicity induced by cancer treatment. <strong>Purpose:</strong> Comparision between the effect of grape seed and fruits of <em>Physalis peruviana</em> methanol extract in reduction of mucositis induced by Cetuximab drug. <strong>Study Design:</strong> Animal study. <strong>Methods:</strong> 60 male rats divided into 6 groups as 10 in each group. Control group of rats with no intervention given. The other 5 groups were given Cetuximab drug alone or with grape seed extract (simultaneously or 1 week before Cetuximab drug) or <em>Physalis </em>extract (simultanously or 1 week before Cetuximab drug). After sacrifice, tongue was dissected and tissue preparation was done for all samples. Digital counting was done by image analyzer computer system for (a) filliform papillae in H&amp;E stained sections, (b) mast cells in mast cell tryptase stained sections to assess inflammation. The area percent for bax immune-expression in other immune stained sections were also measured done to assess apoptosis. <strong>Results:</strong> The difference between the groups was assessed for statistical significance (P&lt;0.0001) by ANOVA test. The greatest mean number of filiform papillae was recorded in control group I. Greatest mean number of mast cells and the greatest mean area percent of bax immune expression were recorded in group II. <strong>Conclusion:</strong> Grape seed extract and <em>Physalis extract</em> could neutralize the harmful effect of cancer therapy. <em>Physalis extract</em> had more potent effect in reducing the inflammation induced by Cetuximab drug.

scholarly journals Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia?

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Grzegorz Szewczyk ◽  
Michał Pyzlak ◽  
Jakub Klimkiewicz ◽  
Wacław Śmiertka ◽  
Magdalena Miedzińska-Maciejewska ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Vascular Network ◽  
Control Group ◽  
Network Development ◽  
Mast Cell Tryptase ◽  
Differential Distribution ◽  
Histamine Concentration ◽  
Common Features ◽  
Mast Cell Density

The physiological course of pregnancy is closely related to adequate development of the placenta. Shallow invasion of trophoblast as well as decreased development of the placental vascular network are both common features of preeclampsia. To better understand the proangiogenic features of mast cells, in this study we aim to identify the potential relationship between the distribution of mast cells within the placenta and vascular network development.Material and Methods. Placentas from preeclampsia-complicated pregnancies () and from physiological pregnancies () were acquired after cesarean section. The concentration of histamine was measured, and immunohistochemical staining for mast cell tryptase was performed. Morphometric analysis was then performed.Results. We noticed significant differences between the examined groups. Notably, in the preeclampsia group compared to the control group, we observed a higher mean histamine concentration, higher mast cell density (MCD), lower mean mast cell (MMCA) and lower vascular/extravascular (V/EVT) index. In physiological pregnancies, a positive correlation was observed between the histamine concentration and V/VEVT index as well as MCD and the V/VEVT index. In contrast, a negative correlation was observed between MMCA and the V/EVT index in physiological pregnancies.Conclusions. Based on the data from our study, we suggest that a differential distribution of mast cells and corresponding changes in the concentration of histamine are involved in the defective placental vascularization seen in preeclamptic placentas.

scholarly journals Effects of long-term occlusal hypofunction and its recovery on the morphogenesis of molar roots and the periodontium in rats

2012 ◽  
Vol 83 (4) ◽  
pp. 597-604 ◽  
Author(s):  
Masahide Motokawa ◽  
Akiko Terao ◽  
Ersan I. Karadeniz ◽  
Masato Kaku ◽  
Toshitsugu Kawata ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Male Rats ◽  
Control Group ◽  
Disuse Atrophy ◽  
Micro Computed Tomography ◽  
Root Area ◽  
Wistar Strain ◽  
Length Width ◽  
Recovery Groups

ABSTRACT Objectives: To investigate the effects of long-term, artificially created, hypofunctional occlusion and its recovery on the morphology of rat molar roots. Material and Methods: Eighteen 5-week-old Wistar-strain male rats were randomly divided according to their periodontal conditions into normal, hypofunctional, and recovery groups (n  =  6 in each). In the experimental hypofunctional and recovery groups, a bite-raising appliance was set to produce hypofunction at the molar region. All groups were analyzed at 16 weeks of age using three-dimensional micro-computed tomography. Root length, width, and area as well as the thickness and the area of the periodontal ligament (PDL) space of the maxillary first molar were calculated. Results: Roots were longer and narrower in the hypofunctional group than in the control group. The mesial root in particular showed a dramatic change. Root area also decreased significantly in the hypofunctional group compared to the other groups. Moreover, the PDL thickness and area decreased significantly in the hypofunctional group compared to the control group, but increased in the recovery group compared to the hypofunctional group. Conclusions: These findings suggest that root size and PDL structure may be reduced due to disuse atrophy resulting from a defect in occlusal function, but may be recovered following a gain of occlusal stimuli.

scholarly journals THE EFFECTIVENESS OF 70% METHANOLIC EXTRACT OF AVOCADO LEAF (Persea americana Mill) IN DECREASING BLOOD SUGAR LEVELS IN MALE RATS (Rattus norvegicus) WISTAR STRAIN INDUCED ALLOXAN

Biomedika ◽  
2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Retno Sintowati ◽  
Anindita Putri Handayani ◽  
Riandini Aisyah
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Methanolic Extract ◽  
Persea Americana ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Wistar Strain ◽  
Sugar Levels

Tanaman alpukat (Persea americana Mill) merupakan tanaman tradisional yang memiliki kandungan flavonoid dan tannin yang dapat menurunkan kadar gula darah. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak metanol 70% daun alpukat (Persea americana Mill) terhadap penurunan kadar gula darah.Metode penelitian menggunakan eksperimental laboratorik dengan rancangan penelitian pretest – posttest with control group design. Hewan uji yang digunakan 25 ekor tikus jantan galur Wistar, umur 2-3 bulan, dan berat badan 150-200 gram yang dibagi dalam 5 kelompok. Kelompok I: kontrol negatif (aquades), kelompok II: kontrol positif (glibenklamid), kelompok III, IV, V : ekstrak metanol 70% daun alpukat dengan dosis berturut-turut adalah sebagbai berikut: 100 mg/ 200 grBB, 150 mg/ 200 grBB, 200 mg/ 200 grBB. Gula darah diukur dengan alat spektrofotometer. Berdasarkan hasil uji ANOVA, data penurunan kadar gula darah hari ke-7 pemberian ekstrak nilai probabilitas signifikan p = 0, 000 dengan demikian p < 0, 05 maka terdapat perbedaan penurunan kadar gula darah yang bermakna. Berdasarkan uji LSD dari semua kelompok didapatkan nilai p < 0,05 yang berarti terdapat perbedaan penurunan kadar gula darah yang signifikan antara2 kelompok. Kesimpulan: Ekstrak metanol 70% daun alpukat (Persea americana Mill) dosis 200 mg/200; 100 mg/200 and 150 mg/ 200 gram/berat badan dapat menurunkan kadar gula darah pada mencit jantan galur wistarKata Kunci : Ekstrak metanol 70%, Alpukat (Persea americana Mill), gula darah, diabetes mellitus.

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