scholarly journals Pengaruh Metode Pengeringan terhadap Aktivitas Antioksidan Daun Alpukat

2019 ◽  
Vol 8 (3) ◽  
pp. 80
Author(s):  
I Wayan Rai Widarta ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Drying Methods ◽  
High Antioxidant Activity

Daun alpukat mengandung komponen bioaktif yang tinggi sehingga dapat dimanfaatkan sebagai sumber antioksidan alami. Tujuan dari penelitian ini adalah mendapatkan metode pengeringan yang tepat sehingga menghasilkan ekstrak daun alpukat dengan aktivitas antioksidan yang tinggi. Daun alpukat baik yang muda maupun yang tua dikeringkan dengan tiga metode pengeringan yaitu dijemur dibawah sinar matahari, dikeringanginkan dalam ruangan, dan dikeringkan dengan oven. Kualitas daun kering kemudian dianalisis aktivitas antioksidan, kadar air, total fenol, total flavonoid, dan total tanin. Hasil penelitian menunjukkan bahwa daun tua yang dikeringkan dengan menggunakan oven pada suhu 40˚C selama 24 jam menghasilkan aktivitas penghambatan radikal bebas tertinggi yaitu 19,83% dengan kadar air 7,54%, total fenol 6,42 mg/100 g ekstrak, total flavonoid 12,07 mg/100 g ekstrak, dan total tanin 2,48 mg/100 g ekstrak. Kesimpulannya, metode pengeringan dapat memberikan dampak terhadap kadar senyawa bioaktif ekstrak daun alpukat dan aktivitas antioksidannya.Effect of Drying Methods on the Antioxidant Activity of Avocado LeavesAbstractAvocado leaves contain high bioactive components that may be used as a source of natural antioxidants. The purpose of this study was to obtain proper drying method to produce avocado leaf extract with high antioxidant activity. Avocado leaves, both young and old, were dried with three drying methods, which were dried in the sun, room, and oven. Quality of leaves was then measured for antioxidant activity, moisture, total phenol, total flavonoid, total tannins. The results showed that old leaves dried using an oven at 40˚C for 24 hours resulted in the highest radical scavenging activity of 19.83% with 7.54% moisture content, 6.42 mg/100 g extract of total phenol, 12.07 mg/100 g extract of total flavonoid, and 2.48 mg/100 g extract of total tannins. In conclusion, the drying methods might produced specific antioxidant activities.

scholarly journals Comparative Study on Chemical Composition and Antioxidant Activity of Annona Muricata Plant Parts Cultivated in Covenant University, Ota, Ogun State, Nigeria

2018 ◽  
Vol 6 (3) ◽  
pp. 807-815
Author(s):  
OMOTOSHO OMOLOLA ELIZABETH ◽  
IHEAGWAM FRANKLYN NONSO ◽  
NOIKI IFEOLUWA ADEBOLA ◽  
OMINI JOY JOHN
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Root Extract ◽  
Plant Parts ◽  
Antioxidant Agents

Annonamuricata plant parts possess a broad range of medicinal and biological properties. This research compared the chemical composition and antioxidant properties of Annonamuric at aparts. Proximate, mineral, total phenol and total flavonoid content as well as invitro antioxidant activity were examined. Results revealed the leaves contained significantly(p<0.05) higher composition of moisture (8.69±0.22%), ash (4.60±0.02%), protein (14.53±0.11%), crude fat (10.28±0.03%),chromium (0.38±0.05 mg/100g), nickel (1.75±0.04mg/100g), total phenol (1.01±0.03mgpyrocatechol/mL) and total flavonoid (1.12±0.03mgGAE/mL)compared to the respective values for root. Carbohydrate (9.29±0.24%), lead (0.13±0.02mg/100g) and cobalt (1.93±0.02mg/100g) composition was significantly lower(p<0.05) in the leaves compared to the respective compositions in the root. The leaf and root extract exhibited a concentration-dependent increase in hydroxyl radical scavenging activity with no observable(p<0.05)difference in their EC50 value. This study suggests the leaves of A. muricata found in Covenant University had better chemical composition when compared to the root. Nonetheless, these plant parts may be further exploited for not only their nutritive composition and mineral content but also a natural source of antioxidant agents.

Effect of Drying Methods on the Antioxidant Activity of Peanut Flour Fermented with Lactobacillus casei LC35

2012 ◽  
Vol 554-556 ◽  
pp. 1095-1098
Author(s):  
Nai Fu Wang ◽  
Wei Qian ◽  
Yi Bin Zhou
Keyword(s):  
Antioxidant Activity ◽  
Lactobacillus Casei ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Peanut Flour ◽  
Drying Methods ◽  
Scavenging Activity ◽  
Freeze Dried

Peanut flour (PF) was fermented with Lactobacillus casei LC35 and then dried using different drying methods in the present study. The effect of drying methods on the antioxidant activities (1, 1-diphenyl-2-picrylhydrazyl [DPPH] radical-scavenging activity, reducing power and Fe2+chelating activity) and flavonoids in fermented-PF (FPF) was investigated. Regardless of the drying methods, the dried FPF showed higher antioxidant activities than PF. Drying methods also showed significant effects on the antioxidant activities of FPF. The freeze-dried FPF had the strongest antioxidant activities compared to the vacuum and hot air-dried FPF. A good linear correlation was found between the EC50value of DPPH scavenging activity (R2 = 0.8972, P < 0.05) or reducing power (R2 = 0.9047, P < 0.05) and the concentration of quercetin in PF and dried FPF. These results indicate the dried FPF, especially for the freeze-dried FPF, might be good functional foods with high antioxidant activity.

CHEMO-PROFILING AND ASSESSMENT OF IN VITRO ANTIOXIDANT EFFICACY OF EIGHT FERNS OF DARJEELING HIMALAYAS, INDIA

2021 ◽  
pp. 25-27
Author(s):  
Nishika Jaishee ◽  
Rohini Lama ◽  
Usha Chakraborty
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Phenolic Contents ◽  
Darjeeling Himalayas

AIM: The present study was performed to prole some phenolics and explore the antioxidant effect of eight locally available ferns collected from different places of Darjeeling Himalayas, India. Methods: The antioxidant activities of methanol (MeOH) extract was evaluated by DPPH free radical scavenging activity. Qualitative analysis of phenol was done using standard methods. Further, characterization of phenolics was done using High performance liquid chromatography. Result: The content of phenolics ranged from 6.77 to 60.066mg FAE/g dry weight. The DPPH antioxidant activity expressed as IC values 50 revealed Nephrolepis cordifolia and Microsorum punctatum to exhibit highest and lowest antioxidative activity respectively. Moderate correlation 2 (R =0.547) was observed between the total phenolics content and antioxidant activity. HPLC analysis of phenolics from all the investigated plants revealed the presence of caffeic acid, ferulic acid and salicylic acid while the other phenolics such as phloroglucinol, gallic acid, pyrogallol, 3,4- dihy droxybenzoic acid, catechol, catechin, chlorogenic acid, caffeine, vanillic acid and cinnamic acid were not uniformly present in all the plants. The phenolic contents values showed wide variation among themselves, as well as within different plants. These ferns with considerable amount of phenolics can be the potential source of natural antioxidants.

scholarly journals Effect of oviposition by Bactrocera dorsalis on the antioxidant activity of orange juice

2020 ◽  
Vol 80 (3) ◽  
pp. 641-647
Author(s):  
M. Ni ◽  
K. Gu ◽  
B. Hassan ◽  
D. Ning ◽  
Y. Zheng ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Orange Juice ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Fruit Fly ◽  
Total Phenol ◽  
Bactrocera Dorsalis ◽  
Total Phenol Content ◽  
Phenol Content

Abstract Among fruits and fruit products, oranges and orange juice are the most widely consumed worldwide. However, the effects of pest infestation of oranges on the quality of orange juice are not yet known. To evaluate the effect of the oriental fruit fly Bactrocera dorsalis on the antioxidant activity of orange juice, we measured changes in the vitamin C (Vc) concentration, total phenol content, and antioxidant activity of orange juice after the introduction of fruit fly eggs. Ten days after the eggs were introduced (larvae removed), the concentration of Vc in orange juice was 18.65 µg/mL, which was 9.16 µg/mL lower than that measured in healthy orange juice. In addition, the total phenol content decreased by 46.519 mg Gallic Acid Equivalents (GAE)/g to 9.748 mg GAE/g. Furthermore, the free-radical scavenging activity decreased from 22.297% to 5.393%. Correlation analysis indicated significant correlations between Vc concentration, total phenol content, and antioxidant activity of orange juice after B. dorsalis infestation. The decrease in Vc concentration, total phenol content and free-radical scavenging activity indicated that B. dorsalis changed the quality of orange juice by affecting the antioxidant activity of the juice after the oranges were infested.

scholarly journals EKSTRAKSI DAUN GEDI (Abelmoschus manihot L) SECARA SEKUENSIAL DAN AKTIVITAS ANTIOKSIDANNYA Antioxidant Activities of Sequentially Extracted Gedi’s (Abelmoschus manihot L) Leaves

2015 ◽  
Vol 35 (03) ◽  
pp. 280 ◽  
Author(s):  
Mercy Teroreh ◽  
Sri Rahardjo ◽  
Pudji Hastuti ◽  
Agnes Murdiati
Keyword(s):  
Singlet Oxygen ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Raw Material ◽  
Polar Component ◽  
Metal Chelating ◽  
Singlet Oxygen Quenching

Gedi’s leave is one of the raw material in tinutuan porridge, a traditional food from Manado Indonesia. The leaves was extracted by various solvent to dissolve all of the compound. The aim of this study was to compare the profile and antioxidant activities of the extract in various sequence of solvent. Hexane, aseton, and methanol were used to dissolve a non polar, semi polar and polar component, respectively. All of the extract were analysed for its antioxidant activities. The profile of extract were indicated by total phenol and total flavonoid meanwhile antioxidant activities wasmeasured by radical scavenging activity DPPH, metal chelating and singlet oxygen quenching. The result indicated the sequence of hexane – aseton – methanol contain the highest of total phenol and flavonoid compare to the others with 10.67±0.49 mg GAE/g extract and 2.33±0.026 mg quersetin/g extract. The sequence also showed the highest of antioxidant activities at 150 μg/mL extract with 67.47%; 48.07% and 38.66% for percentage of inhibition DPPH, value of metal chelating and singlet oxygen, respectively.Keywords: Gedi’s leave, sequencial extraction, phenolic, flavonoid and antioxidant ABSTRAKDaun Gedi (Abelmoschus manihot L) merupakan salah satu bahan utama tinutuan, makanan tradisional Manado.Penelitian tentang profil dan aktivitas antioksidan dari daun Gedi telah dilakukan. Tujuan penelitian ini adalah mengevaluasi dan membandingkan profil dan aktivitas antioksidan dari daun gedi yang diekstraksi secara sekuensial dengan pelarut heksana, aseton dan metanol. Ekstrak daun gedi selanjutnya dianalisis kandungan total fenolik dan flavonoid, sedangkan aktivitas antioksidannya dilakukan secara in vitro meliputi penangkal radikal bebas DPPH, pengkelat logam dan penstabil oksigen singlet. Hasil penelitian menunjukkan bahwa ekstrak sekuensial heksanaaseton-metanol(ESHAM) memiliki total fenol dan total flavonoid yang tertinggi dibandingkan dengan ekstrak lainnya,masing-masing sebesar 10,67±0,49 mg GAE/g ekstrak dan 2,33±0,026mg kuersetin/g ekstrak. ESHAMjuga memiliki aktivitas antioksidan yang paling tinggi, dengan persentase penghambatan DPPH sebesar 67,47%; persen pegkelat logam sebesar48,07% dan persen penghambatan oksigen singlet sebesar 38,66% pada konsentrasi 150μg/mL esktrak. Kesimpulan senyawa fenolik pada daun gedi bersifat polar sehingga menghasilkan aktivitas antioksidan tertinggi pada pelarut polar.Kata kunci: Daun gedi, ekstraksi sekuensial, fenolik, flavonoid dan antioksidan  

scholarly journals Phenolic and Flavonoid Content and Antioxidant Activity of Daphne Blagayana Growing in Serbia

2014 ◽  
Vol 15 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Nedeljko Manojlovic ◽  
Miroslav Sovrlic ◽  
Pavle Maskovic ◽  
Perica Vasiljevic ◽  
Marina Juskovic
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
First Time ◽  
Phenolic And Flavonoid

ABSTRACT Th e aim of this study was to examine the phenolic and flavonoid contents and antioxidant activities of methanol and chloroform extracts of leaves and twigs of Daphne blagayana. Th e total phenolic content in the chloroform extract of plant twigs (90.26 ± 0.69 mg GA/g) was higher than that of the other extracts (from 76.56±0.89 to 77.45±0.43 mg GA/g). In the case of flavonoids, a greater value was also obtained for the chloroform extract of twigs (35.24±0.55 mg RU/g). Several diff erent methods were used to determine the antioxidant activity of the tested extracts, including total antioxidant capacity, metal chelating activity, hydroxyl radical scavenging activity and inhibitory activity against lipid peroxidation. Our results showed that although secondary metabolites of the plants may contribute significantly to their antioxidant activities, those antioxidant activities were not directly related to the phenolic and flavonoid amounts. Th e results of the present analysis demonstrated, for the first time, that Daphne blagayana leaves and twigs possess high phenolic and flavonoid contents, as well as potential antioxidant activity. Th is study suggests that Daphne blagayana twigs and leaves may potentially be used as an accessible source of natural antioxidants.

Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antimicrobial Activities ◽  
Total Flavonoid ◽  
Root Extract ◽  
Antioxidant Power ◽  
Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

2011 ◽  
pp. 151-157 ◽  
Author(s):  
Marijana B. Saka ◽  
Julianna F. Gyura ◽  
Aleksandra Mišan ◽  
Zita I. Šereš ◽  
Biljana S. Pajin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Shelf Life ◽  
Dietary Fiber ◽  
Wheat Flour ◽  
Room Temperature ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

scholarly journals Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
K. Nagendra Prasad ◽  
Jing Hao ◽  
Chun Yi ◽  
Dandan Zhang ◽  
Shengxiang Qiu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Liver Carcinoma ◽  
Scavenging Activity ◽  
Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

2021 ◽  
Author(s):  
E.K. Soltani ◽  
K. Zaim ◽  
K. Mokhnache ◽  
N. Haichour ◽  
S. Mezaache-Aichour ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Aqueous Extracts

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

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