scholarly journals Effects of radiofrequency on muscle tissue regeneration

2020 ◽  
Vol 18 ◽  
pp. 1-8
Author(s):  
Rodrigo Marcel Valentim Da Silva ◽  
Rafael Limeira Cavalcanti ◽  
Bruno Felipe Soares Félix ◽  
Rodney Wordnei Coutinho Santos ◽  
Tamara Martins Da Cunha ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Maximum Load ◽  
Biomechanical Properties ◽  
Optical Microscope ◽  
Sine Wave ◽  
The Other ◽  
Control Group ◽  
Intact Muscle ◽  
Traction Test ◽  
Gastrocnemius Muscles

Background: Radiofrequency (RF) is recommended to treat pathologies with the presence of inflammation, as it induces diathermyand, consequently, promotes better oxygenation, nutrition and local vasodilation. Objective: Evaluate the effect of RF on muscleregeneration in Wistar rats. Methods: It is a controlled and randomized experiment, with a sample composed of 40 Wistar rats,divided equally into four groups: G1 (control group), G2 (lesion, without RF), G3 (RF after 72 hours of lesion) and G4 (RF after 7 daysof lesion), all sacrificed 21 days after the injury. The RF parameters used were: Sine wave; frequency of 0.5MHz; 5 cm² treatmentarea on the region around the lesion; power of 45%; two-minute application; intensity of 15 seconds to heat the head, 1 minute at20% and another minute at 10%. An optical microscope was used for histological analysis and, for the biomechanical properties(maximum elongation and maximum load), the mechanical traction test of the gastrocnemius muscles. For statistical analysis, thetwo-way ANOVA test and the Benferroni test were used, considering 5% of significance. Results: It was observed in G3 that theinflammatory process was optimized by the RF when compared to the other groups, presenting intact muscle fibers with a discreetregeneration process. G4, on the other hand, revealed intense inflammation with significant granulation tissue, as well as fibrosis andhealing. As for the biomechanical characteristics, there were no statistically significant differences in the intergroup comparison.Conclusion: RF was more effective when applied after 72 hours after the injury, in addition to not interfering with musclebiomechanical characteristics.

scholarly journals Evaluation of intracanal acetazolamide in late reimplanted rat teeth

2017 ◽  
Vol 11 (04) ◽  
pp. 417-421
Author(s):  
Camila Paiva Perin ◽  
Vula Papalexiou ◽  
Aline Cristina Batista Rodrigues Johann ◽  
Natanael Henrique Ribeiro Mattos ◽  
Fernando Henrique Westphalen ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Calcium Hydroxide ◽  
Wistar Rats ◽  
Root Resorption ◽  
Optical Microscope ◽  
Physiological Solution ◽  
The Other ◽  
Control Group ◽  
Hematoxylin And Eosin

ABSTRACT Objective: The aim of this study was to evaluate the use of acetazolamide combined with different agents as intracanal medication in late reimplanted rat teeth. Materials and Methods:In 100 Wistar rats, divided into 5 groups of 20, one of the following medications was used: Acetazolamide liquid (AL); AL with calcium hydroxide powder (ALHC); acetazolamide powder with AL; acetazolamide powder with physiological solution; and calcium hydroxide with physiological solution (control). At 30 and 60 days after reimplantation, the animals were sacrificed, tissues were processed, and cuts were stained with hematoxylin and eosin. An optical microscope was used to determine the following: percentage of inflammatory root resorption (RRI); percentage of substitute root resorption (RRS); and presence of ankylosis. The data obtained was submitted for statistical analysis. Results: Group ALHC had a significantly higher RRS than the control group at 60 days (P = 0.01). Group AL showed significantly less ankylosis than the other groups, including the control, at 30 days. AL showed results similar to those of the control group with respect to RRS. Conclusion:Acetazolamide has the potential to be an effective intracanal medication.

scholarly journals Effect of the oral administration of monosodium glutamate during pregnancy and breast-feeding in the offspring of pregnant Wistar rats

2010 ◽  
Vol 25 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Vinicius von Diemen ◽  
Manoel Roberto Maciel Trindade
Keyword(s):  
Weight Gain ◽  
Oral Administration ◽  
Wistar Rats ◽  
Monosodium Glutamate ◽  
The Other ◽  
Control Group ◽  
Pregnant Rats ◽  
Dependent Relation ◽  
Dose Dependent

PURPOSE: Determine the effects of the MSG (monosodium glutamate) in the offspring of pregnant rats through the comparison of the weight, NAL (nasal-anal length) and IL (Index of Lee) at birth and with 21 days of life. METHODS: Pregnant Wistar rats and their offspring were divided into 3 groups: GC, G10 and G20. Each of the groups received 0%, 10% and 20% of MSG, respectively from coupling until the end of the weaning period. RESULTS: Neither weight nor NAL were different among the groups at birth. The group G20 at birth had an IL lower than the group GC (p<0,05) and with 21 days of life presented weight and NAL lower than the groups G10 and this lower than the GC (p<0,01). Otherwise the G20 at 21 days of life had the IL similar to the other two groups. The weight profit percentage from birth to the 21st day of life was lower in the G20 regarding the other two groups (p<0,01). The G20 had a NAL increase percentage from birth to the 21st day of life lower than the G10 and this lower than the GC (p<0,01). CONCLUSIONS: MSG presented a dose-dependent relation in the variables weight and NAL. It caused a decrease in the growth pattern as well as in the weight gain pattern until the 21st day of life. The IL of the group 20% had an increased in relation to the control group after 3 weeks of follow up.

scholarly journals Analysis of biomechanical properties of tibias after bone failure and ozone treatment in rats

2020 ◽  
Vol 9 (9) ◽  
pp. e530997474
Author(s):  
Alexadre Aniceto Rodrigues ◽  
Thiago Donizeth da Silva ◽  
Evelise Aline Soares ◽  
José Antônio Dias Garcia ◽  
Flavia da Ré Guerra ◽  
...  
Keyword(s):  
Mechanical Testing ◽  
Structural Properties ◽  
Wistar Rats ◽  
Biomechanical Properties ◽  
Bone Defects ◽  
Biomechanical Analysis ◽  
Maximum Force ◽  
Ozone Treatment ◽  
Control Group ◽  
Tissue Healing

Introduction: Ozone is a potent antioxidant that acts as a precursor of various radicals, being indicated as a powerful therapy, assisting in the process of tissue healing. Objectives: The proposal of this study was to analyze material and structural properties via mechanical testing in tibias after application of ozone in bone defects produced surgically. Methods: Ten male 40-day old albino Wistar rats have been used, divided in two groups: control group and ozone group, this last one being treated with ozonized water in 25 µg/mL of concentration until the day of euthanasia. Trichotomy and longitudinal incision was conducted in the animals’ leg skin, exposing the tibia’s diaphysis of both antimers, and with help of a high rotation pen a flaw has been produced on the bone. After 60 days of surgery the animals were euthanized, and tibias were collected for biomechanical analysis. Results: The results of the biomechanical properties – structural and material – evidenced significant interactions through exposure to ozone, showing a diminished bone resistance in animals from the control group, observed by the decrease of the maximum force (N) needed to rupture the bone when compared to the value needed to break the bones of the animals from the ozone group, and the analysis of the morphometrical properties did not show any difference between both experimental groups. Conclusion: The use of ozone did not alter the morphological structures of the tibias, and the group which used ozone presented more resistance during mechanical testings, because the maximum force for the rupture of tibia was greater in this group.

scholarly journals UVB Dose Optimization for Phototherapy in Vitamin D Deficiency : Profile Analysis of Vitamin D, TNF-α, Vascular Endothelial Growth Factor (VEGF) and Platelet Derived Growth Factor (PDGF) in Wistar Rats

2020 ◽  
Vol 19 (4) ◽  
pp. 749-754
Author(s):  
Cynthia Arsita ◽  
Taufiqurrachman Nasihun ◽  
Atina Hussaana
Keyword(s):  
Vitamin D ◽  
Growth Factor ◽  
Wistar Rats ◽  
Biological Effects ◽  
Medical Science ◽  
The Other ◽  
Control Group ◽  
Uvb Radiation ◽  
Tnf Α ◽  
Post Radiation

Background : UVB radiation responsible for the most important biological effects including Vitamin D3 synthesis and inflammation. UVB radiation are absorbed by 7-dehydrocholesterol in the plasma membrane of epidermal cells resulting in production of cis-previtamin D3. In the other hand, an exposure to UVB leads to cutaneous tissue inflammation modulates by TNF-α which also increases platelet activating factor. VEGF and PDGF induced by TNF-α during wound healing, characterized with angiogenesis and reephitalization. Furthermore, vitamin D plays a role in inflammation inhibition and upregulates growth factors. However, the study of the mechanism has not yet been thoroughly investigated. Methods: This study uses post test only group design, subjected wistar rats divided into four groups. Control group, non irradiated with UVB, and the other three groups, treated with graded UVB dose started with 1 MED (50 mJ/cm2), 2 MED (100mJ/cm2) and 3 MED (150 mJ/cm2) and investigated at 6, 12, 24 and 48 hours post UVB irradiation. Result : The serum level of vitamin D, VEGF and PDGF were increasing due to UVB dose addition. The highest level was reached at 6 hours post radiation using 3 MED, which gradually decrease up to 48 hours (p =0,000). The rise of vitamin D after UVB radiation, inhibit TNF-α induction in every dose accordant UVB dose addition and the lowest level is using 3 MED at 12 hours post radiation (p =0,000). TNF-α reach its highest level at 24 hours post radiation using 1 MED, it is related with the acute phase of inflammation. Conclusion : This study reveal that higher UVB irradiance increases vitamin D and inhibit TNF-α which also promotes VEGF and PDGF. Bangladesh Journal of Medical Science Vol.19(4) 2020 p.749-754

scholarly journals Morphological changes in distant muscle fibers following thermal injury in Wistar rats

2010 ◽  
Vol 25 (6) ◽  
pp. 525-528 ◽  
Author(s):  
Flavia de Oliveira ◽  
Luci Rezende Bevilacqua ◽  
Carlos Alberto Anaruma ◽  
Silvia de Campos Boldrini ◽  
Edson Aparecido Liberti
Keyword(s):  
Body Surface ◽  
Wistar Rats ◽  
Thermal Injury ◽  
Morphological Changes ◽  
Muscle Fibers ◽  
The Body ◽  
Medial Gastrocnemius ◽  
Control Group ◽  
Hematoxylin And Eosin ◽  
Gastrocnemius Muscles

PURPOSE: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. METHODS: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. RESULTS: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. CONCLUSION: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.

scholarly journals Effect of single clove garlic on clinical symptoms of photoaging in wistar rats exposed with UV-B

2019 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
Nyoman Pratiwi Hapsari Dewi ◽  
I Gusti Ayu Dewi Ratnayanti ◽  
I Gusti Kamasan Nyoman Arijana ◽  
Ni Made Linawati
Keyword(s):  
Clinical Symptom ◽  
Wistar Rats ◽  
Clinical Symptoms ◽  
Light Exposure ◽  
Premature Aging ◽  
The Other ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Photoaging is a premature aging that occurs on skin due to the ultraviolet light exposure that causes the emergence of clinical symptoms, one of which is wrinkle. One kind of material that can prevent photoaging is antioxidant. A single clove garlic has the highest antioxidant activity compared to the other materials. Aim: This research aimed at investigating the effectivity of single clove garlic in inhibiting the clinical symptom of photoaging. Method: This research was an experimental research, utilizing pre-post-test control group design. The used sample were 30 male wistar rats which were divided into 6 experimental groups. All groups were exposed to UV-B light with the amount of 840 mJ/cm2. Control group (P1) was only exposed to UV-B while the other groups were treated with placebo cream (P2), sunblock (P3), 5% garlic cream (P4), 10% garlic cream (P5) and 20% garlic cream (P6) respectively. The clinical symptom in the form of wrinkle was observed by using dermascope and the observations were categorized based on Glogau Scale. The statistical analysis utilized Wilcoxon and Kruskal Wallis test which was followed by Mann Whitney test. Result: The result of the research showed that there was significant difference on the apparent wrinkle on P1 group and P3, P5 and P6. Meanwhile the comparison between P1, P2 and P4 was not significant. Conclusion: The single clove garlic extract can prevent photoaging and has the similar protective effect for the skin as of sunblock.

scholarly journals Viability of Equine Semen Stored in a Polyethylene System for Transport for Eight Hours

2017 ◽  
Vol 45 (1) ◽  
pp. 6
Author(s):  
Alisson Ceccato Maciel ◽  
Vinicius Camargo ◽  
Rodrigo Costa Mattos ◽  
Sandra Fiala Rechsteiner
Keyword(s):  
Plasma Membrane ◽  
Sperm Morphology ◽  
Storage Temperature ◽  
Optical Microscope ◽  
The Other ◽  
Semen Sample ◽  
Cooling Curve ◽  
Control Group ◽  
Mean Values ◽  
Significant Difference

Background: Equine semen storage and shipment, being it colled or frozen, allows the veterinarian to direct matings, providing the use of genetically superior stallions, which are mostly located in breeding stations or training centers. Achieving good pregnancy rates depends, beyond the moment of artificial insemination (AI), on factors related to the semen cooling, such as: system used for transport, cooling rate, final storage temperature, storage time and individual variation among stallions, such as age and resistance to cooling. Based on these aspects, this experiment was conducted in order to test a polyethylene system to ship equine semen.Materials, Methods & Results: A total of 87 ejaculates from five stallions with known fertility were used. The stallions aged between 6 to 14 years old, being three Thoroughbred and two Miniature Pony horse. The ejaculates were collected twice a week using a Hannover artificial vagina. After each collection, the semen sample was macroscopically evaluated for appearance, color and smell. A semen sample was used to evaluate the parameters of total motility, vigor and concentration, being these last three parameters assessed by counting 100 sperm cells for analysis. These analysis were performed using an optical microscope, being the concentration taken with a Neubauer chamber after dilution of 1:20 (semen: citrate formol). Subsequently to this, the semen was diluted 1+1 (diluent+semen) with skim UHT milk, and divided into four aliquots of equal volume, yielding a total of four groups. In the control group (GC) the semen was analyzed immediately after dilution (zero h - 0-h). Samples from other groups were stored for eight h (8-h) in three different devices: Equitainer® (GE), BotuFLEX® (GB) or Polyethylene System - Cooled (SP) and non-Cooled (SPN). During storage of the samples the cooling curve of the system under study was evaluated, using a digital thermometer. To estimate the sperm viability, the parameters of total sperm motility and vigor were evaluated, the integrity of plasma membrane  were evaluated using CFDA / PI, and the plasma membrane functionality using the hiposmotic test (HOST), being also examined the percentage of spermatozoa considered morphologically normal (sperm morphology). The SP showed a PM integrity and sperm morphology as the only parameters that did not occur a statistical difference  when compared to Equitainer® and the BotuFLEX® (averages of the CFDA/PI = 71.12%, 73.29%, 71.32 and averages of morphology = 80.50%, 82.29% and 81.28%, respective values of each parameter to SP, GE and GB, while SPN showed mean values lower than the GC and GE for all parameters (averages of total motility = 50.65% and 62, 60%; averages of vigor = 1.78 and 2.43; averages of CFDA/PI = 72.56% and 78.30%; averages of HOST = 43.56% and 48.87%; averages of morphology = 77.17% and 84.82%, respectively for SPN and GE). Compared to BotuFLEX®, the SPN had no significant difference only for the parameter of PM functionality (HOST).Discussion: The average temperature of SP was 18.0°C, and the SPN was 18.2°C. The SP was equal or similar to the other used systems, but the SPN showed no difference when compared to PS. However NPS was worse than the other systems from this experiment. These results show that the PS might be an alternative to ship equine semen for eight h, compared to the other cooled systems studied in this work.

scholarly journals Gossypol Promotes Degeneration of Ovarian Follicles in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ivana Cristina Nunes Gadelha ◽  
Michelly Fernandes de Macedo ◽  
Sílvia Catarina Salgado Oloris ◽  
Marilia Martins Melo ◽  
Benito Soto-Blanco
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Serum Levels ◽  
Ovarian Follicles ◽  
The Other ◽  
Control Group ◽  
Stages Of Development ◽  
Hormone Levels ◽  
Stage Of Development ◽  
Female Wistar Rats

The present study aimed to determine if gossypol interferes with ovarian follicles in rats. Twenty-four female Wistar rats were assigned to two equal groups: one control group and the other dosed with gossypol (25 mg/kg/day, subcutaneously) for 15 days. Ovarian follicles were histologically classified according to the stage of development and as normal or atretic. Gossypol treatment reduced the length of estrous with an increase in the duration of the diestrus phase. This compound was responsible for reduced serum levels of T4 and progesterone. Treatment with gossypol was responsible for a significant reduction in the number of normal ovarian follicles and a significant increase in the number of atretic follicles, both in all stages of development. Thus, treatment of rats with gossypol was responsible for reduction in the number of viable follicles and changes in hormone levels that resulted in interference of the estrous cycle.

scholarly journals Bagian Biomedis Fakultas Kedokteran Gigi, Universitas Jember Jember, Indonesia

2011 ◽  
Vol 10 (1) ◽  
pp. 26
Author(s):  
I Dewa Ayu Ratna Dewanti
Keyword(s):  
Traditional Medicine ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
The Other ◽  
Control Group ◽  
Aqueous Extracts ◽  
Treatment Groups ◽  
Neem Leaves ◽  
Significant Difference ◽  
Phagocytosis Activity

Neem is one of the traditional medicine known by society as immunomodulator. On the other hand, 80% of oralinfection diseases is caused by C. albicans. This research is aimed to explain the phagocytosis activities on wistarrats which were inoculated with C. albicans and fed with neem leaves. There are 5 groups, namely control group(KO)with no treatment, the other treatment groups were classified into 4 groups. First group (KP1)was inoculatedwith C. albicans only. KP2 was fed with 50 mg/day/kg BW aqueous extracts from neem leaves, then inoculated withC. albicans start from day 8-21. KP3 was fed with 100 mg/day/kg BW aqueous extract from neem leaves, theninoculated with C. albicans start from day 8-21. KP4 was fed with 200 mg/day/kg BW aqueous extract from neemleaves, then inoculated with C. albicans start from day 8-21. On day 22, the tongue was swabbed for each group,then cut for immunohistochemistry preparation. The study that there was significant difference showed that therewere different results computed using anova, HSD test, and linier regression. The conclusion was neem leavesincreased the phagocytosis activity of wistar rats, inoculated with C.albicans.

scholarly journals The Effects of Administration of Mangosteen Pericap's Ethanolic Extract and Xanthone on Angiogenesis of Gastric Ulcer Healing in Wistar Rats Observed Through the Increase in the level of NO and VEGF and CD-31 Expressions

2011 ◽  
Vol 3 (3) ◽  
pp. 191
Author(s):  
Ika Kustiyah Oktaviyanti ◽  
Djanggan Sargowo ◽  
Mohammad Aris Widodo ◽  
Karyono Mintaroem
Keyword(s):  
Gastric Ulcer ◽  
Treatment Group ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Immunohistochemical Analysis ◽  
The Other ◽  
Gastric Ulcers ◽  
Control Group ◽  
Cd 31 ◽  
Post Test

BACKGROUND: NSAIDs can cause gastric ulcer or may delay the healing of it. Upon exposure to indomethacin, gastric ulcer can occur due to oxidants. Mangosteen rind contains xanthone, which is a natural antioxidant. Administration of this antioxidant may increase angiogenesis that can accelerate healing of gastric ulcer.METHODS: This study used an experimental method with randomized post test control only design using Wistar rats. The rats were put on fasting for 24 hours, then a single dose of 30mg/kg body weight (BW) Indomethacine was given. The rats were divided into control group and treatment group. The treatment group was further divided into two subgroups: one group was given a daily 200 mg/kg BW mangosteen pericap extract, and the other group was given 35 mg/kg BW Xanthone. Both the control group and treatment group were decapitated on the 3rd day, 6th day and 12th day, respectively. After decapitation, the stomach of each rat was taken and divided into two portions, one portion was used for NO examination by ELISA, and the other portion for hispathological examination and immunohistochemical analysis for assessing CD 31 and VEGF expressions.RESULTS: Administration of mangoosteen pericap and xanthone could accelerate healing of gastric ulcers as compared with the control, as shown by the decrease in the severity level of the ulcers. Mangoosteen pericap and xanthone could also increase NO, VEGF expression, and CD-31 as compared with the control, especially on the 3rd day of treatment. Explanation of this finding might be that the antioxidants contained in the mangoosteen pericap or in xanthone could bind with radical superoxide and accelerate release of free NO. The increase of NO caused increase of VEGF and CD-31 that could accelerate angiogenesis, which eventually could accelerate healing of the gastric ulcers.CONCLUSIONS: The effect of mangosteen pericap's extract and xanthone can improve healing of gastric ulcers by increasing nitric oxide and also can increase angiogenesis as shown by the increase of VEGF and CD-31 expressions.KEYWORDS: mangosteen, healing, gastric Ulcer, angiogenesis

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