scholarly journals The state of systemic immunity under the influence of polarized light and an immunomodulator in patients with HPV-associated cervicitis

2021 ◽  
Vol 19 (5) ◽  
pp. 286-292
Author(s):  
Gulnara. G Moseshvili ◽  
Natalia B. Korchazhkina ◽  
Madina Z. Dugieva ◽  
Anna A. Mikhailova ◽  
Vadim V. Portnov
Keyword(s):  
Peripheral Blood ◽  
Carotid Arteries ◽  
Polarized Light ◽  
Relative Content ◽  
The State ◽  
Control Group ◽  
Positive Trend ◽  
Incoherent Light ◽  
Systemic Immunity ◽  
Significant Difference

Background. According to current epidemiological studies, 94.9% of women suffering from sexually transmitted infections are diagnosed with the presence of human papillomavirus (HPV) DNA during the examination. Aims. The aim of the study was to study the effect of combined exposure to blue monochromatic polarized incoherent light and polychromatic visible and infrared polarized light in combination with imihimod 5% cream for external use on the state of systemic immunity in patients with HPV-associated cervicitis. Materials and methods. The study included 60 patients aged 2035 years with an HPV-associated cervicitis with a history of at least 1 year, who were randomly divided into 3 groups: in the main group (n=20), course effects of blue monochromatic polarized incoherent light on the projection of the carotid arteries and polychromatic visible and infrared polarized light on the cervix in combination with imihimod 5% cream for external use (complex 1); in the comparison group (n=20) exposure to polychromatic visible and infrared polarized light on the cervix in combination with imihimod cream for external use 5% (complex 2); in the control group (n=20) a course of local exposure to the cervix with imihimod cream 5%. The data of the survey of 20 healthy women of the same age were taken as the values of the norm. The state of systemic immunity in patients with HPV-associated cervicitis before treatment and after the course was evaluated by the indicators of mature T-lymphocytes (CD3+), CD4+, CD8+, immunoregulatory index (IRI = CD4+/CD8+), CD16+ (natural killer cells) and CD20+ (B-mature lymphocytes) in peripheral blood. Results. Before the start of treatment, the patients showed low levels of CD3+-lymphocytes and a decrease in the immunoregulatory index, which is the ratio of CD4+/CD8+ against the background of an increase in the relative content of CD16+ and CD20+, which indicates an imbalance in the cellular immunity. After the course of treatment, the most pronounced results were obtained under the influence of complex 1: a significant increase in the relative content of CD3+ and normalization of IRI against the background of a decrease to the reference values of the initially elevated levels of CD16 (%) and CD20 (%) in both the percentage and absolute ratio of these populations in peripheral blood. Patients treated with complex 2 also showed a positive, but less pronounced, trend towards an increase in CD3+ and IRI and a decrease in elevated CD16+ levels after the course of treatment. In the control group, there was no statistically significant difference between the indicators before and after treatment, and only a slight positive trend was observed for some of them. Conclusion. The combined use of blue monochromatic polarized incoherent light on the projection of the carotid arteries in combination with polychromatic visible and infrared polarized light and imihimod 5% cream for external use on the cervix in patients with chronic cervicitis associated with papillomavirus infection, to a greater extent than the local use of polarized light in combination with imihimod 5%, has a pronounced immunocorrective effect on the cellular link of immunity.

scholarly journals Modern approaches to the application of photoimmunomodulating technologies in the treatment of HPV-associated cervicitis

2021 ◽  
Vol 18 (3) ◽  
pp. 166-171
Author(s):  
Gulnara G. Moseshvili ◽  
Madina Z. Dugieva ◽  
Natalya B. Korchazhkina ◽  
Anna A. Mikhailova
Keyword(s):  
Topical Application ◽  
Uterine Cervix ◽  
Carotid Arteries ◽  
Comparison Group ◽  
Normal Values ◽  
Polarized Light ◽  
Main Group ◽  
Control Group ◽  
Incoherent Light ◽  
External Application

We aimed to investigate the effects of the combined use of polychromatic visible and infrared polarized light with topical application of Imiquimod 5% cream on the macrophage link of immunity in patients with chronic human papillomavirus (HPV)-associated cervicitis. Materials and methods. The study included 90 patients aged 2035 years, who had a history of HPV-associated cervicitis for at least 1 year. Patients were distributed into three groups by random sampling. The main group included 30 patients who were exposed to blue monochromatic polarized incoherent light on the projection of the carotid arteries and polychromatic visible and infrared polarized light on the uterine cervix in combination with topical application of Imiquimod 5% cream (complex one). The comparison group included 30 patients who were exposed to polychromatic visible and infrared polarized light on the uterine cervix in combination with Imiquimod 5% cream (complex 2), and the control group included 30 patients who received local exposure to the uterine cervix with Imiquimod 5% cream. Examination data of 20 healthy women of the same age were taken as normal values. The macrophage link activity was assessed using the nitroblue tetrazolium test. Results. Under the influence of the developed complexes 1 and 2, the percentage of phagocytic cells of the macrophage link increased significantly to normal values (by 36% and 31%, respectively; p 0.01), the phagocytic number and index of phagocytosis completeness increased to near-normal values (p 0.001). In addition, the percentage of spontaneous (excessive) redox processes of neutrophils decreased from 21.1% 1.3% to 11.8 1.01% in the main group (p 0.001) and to 14.4 1.5% in the comparison group (p 0.01). Less significant results were obtained in the control group where the patients received topical application of Imiquimod 5% cream on the uterine cervix, and the studied parameters were 26%51.7% lower than that in the main group. Conclusion. The complex application of blue monochromatic polarized incoherent light on the projection of the carotid arteries in combination with polychromatic visible and infrared polarized light and external application of Imiquimod 5% cream on the cervix of patients with chronic HPV-associated cervicitis has a pronounced immunocorrecting effect on the immunity macrophage link. This is confirmed by an improvement in the indicators of the functional activity of neutrophils, in the form of a decrease in the percentage of excessive redox reactions and an increase in the index of phagocytosis completeness, and indicates an increase in the productivity of the macrophage link functioning.

scholarly journals Clinical observations of bone marrow transfusion for promoting bone marrow reconstruction after chemotherapy for AIDS-related lymphoma

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yixuan Liu ◽  
Suhong Xie ◽  
Lei Li ◽  
Yanhui Si ◽  
Weiwei Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Immune System ◽  
T Lymphocytes ◽  
Peripheral Blood ◽  
Autologous Bone Marrow ◽  
Control Group ◽  
Peripheral Vein ◽  
Significant Difference ◽  
Autologous Bone ◽  
Aids Related Lymphoma

Abstract Background This study investigates the effect of autologous bone marrow transfusion (BMT) on the reconstruction of both bone marrow and the immune system in patients with AIDS-related lymphoma (ARL). Methods A total of 32 patients with ARL participated in this study. Among them, 16 participants were treated with conventional surgery and chemotherapy (control group) and the remaining 16 patients were treated with chemotherapy followed by autologous bone marrow transfusion via a mesenteric vein (8 patients, ABM-MVI group) or a peripheral vein (8 patients, ABM-PI group). Subsequently, peripheral blood and lymphocyte data subsets were detected and documented in all patients. Results Before chemotherapy, no significant difference in indicators was observed between three groups of ARL patients. Unexpectedly, 2 weeks after the end of 6 courses of chemotherapy, the ABM-MVI group, and the ABM-PI group yielded an increased level of CD8+T lymphocytes, white blood cells (WBC), and platelet (PLT) in peripheral blood in comparison to the control group. Notably, the number of CD4+T lymphocytes in the ABM-PI group was significantly higher than that in the other two groups. Additionally, no significant difference in haemoglobin levels was observed before and after chemotherapy in both the ABM-MVI and ABM-PI groups, while haemoglobin levels in the control group decreased significantly following chemotherapy. Conclusions Autologous bone marrow transfusion after chemotherapy can promote the reconstruction of both bone marrow and the immune system. There was no significant difference in bone marrow recovery and reconstruction between the mesenteric vein transfusion group and the peripheral vein transfusion group.

scholarly journals The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study

2020 ◽  
Author(s):  
Bahare Keshavarzi ◽  
Meraj Tabatabaei ◽  
Amir Hasan Zarnani ◽  
Fahime Ramezani Tehrani ◽  
Mahmood Bozorgmehr ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Amniotic Membrane ◽  
Peripheral Blood ◽  
Normal Pregnancy ◽  
Interleukin 4 ◽  
Control Group ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Dendritic Cell Differentiation ◽  
Significant Difference

Background: The amniotic membrane plays an important role in maintaining a healthy pregnancy. The main population cells from amniotic membrane include human amnion epithelial cells (hAECs) which have been shown to possess immunomodulatory properties. Objective: The proximity of hAECs with monocyte leads to the generation of tollerogenic dendritic cells. Materials and Methods: hAECs were obtained from normal pregnancy. Peripheral blood monocytes were isolated by anti-CD14 MACS method. Co-cultures of monocytes and hAECs were established in Transwell chambers supplemented with granulocytemacrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) in the absence and presence of lipopolysaccharide (LPS) to produce immature and mature DCs, respectively. Immunophenotyping of the obtained DCs was done through flow cytometry and the production of cytokines was measured by ELISA. Mixed leukocyte Reaction (MLR) was also performed for the functional assessment of DCs. Results: Immunophenotyping of [hAECs - Immature DC (iDC)] and [hAECs - iDC] + LPS cells revealed that the expression of CD1a, CD80, CD86, CD40, HLA-DR, and CD83 markers showed no significant difference as compared with the control group (iDCs and mDCs alone). In the [hAECs-iDCs] + LPS cells, the percentage of CD14 cells at the ratio of 1:2.5 showed significant differences compared to the control group. The production of IL-10 and IL-12 showed no significant difference in any of the cultures as compared to the control groups. Also, co-cultured DCs did not inhibit proliferation of lymphocyte. Conclusion: Our findings show that factors secreted from cultured hAECs are unable to generate of tollerogenic dendritic cells. To achieve a better understanding of other mechanisms more investigations are needed. Key words: Amniotic membrane, Dendritic cells, Human placenta, Immunomodulation, Monocyte.

scholarly journals Correlation between expression levels of IL-37, GM-CSF, and CRP in peripheral blood and atherosclerosis and plaque stability

2019 ◽  
Vol 17 ◽  
pp. 205873921984406
Author(s):  
Tao Zheng ◽  
Qingyun Zhou ◽  
Zhe Chen ◽  
Qinning Wang
Keyword(s):  
Peripheral Blood ◽  
Density Lipoprotein ◽  
Inflammatory Factors ◽  
Control Group ◽  
Case Group ◽  
Expression Levels ◽  
Gm Csf ◽  
Significant Difference ◽  
Group A ◽  
Group B

The study aimed to study the correlation between expression levels of interleukin-37 (IL-37), granulocyte macrophage colony-stimulating factor (GM-CSF), and C-reactive protein (CRP) in peripheral blood and the status of atherosclerosis (AS) and plaque stability and to confirm the clinical significance of these inflammatory factors in the pathogenesis of AS. A total of 64 AS patients (case group) were selected and divided into unstable plaque group (group A, 28 cases) and stable plaque group (group B, 36 cases) according to the color ultrasonography results of arterial vessels. At the same time, 30 healthy subjects were classified into the control group. General information of the enrolled subjects was collected, including levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), CRP, and homocysteine (Hcy). The expression levels of IL-37 and GM-CSF in the serum of peripheral blood samples collected from these subjects were measured by enzyme-linked immunosorbent assay (ELISA). There was no significant difference between the case group and the control group in the levels of TC, TG, HDL, and LDL ( P > 0.05). However, the expression level of Hcy in the case group was significantly higher than that in the control group ( P < 0.05). Compared with the control group, the expression levels of IL-37, GM-CSF, and CRP in the case group were significantly increased ( P < 0.05). In addition, compared with group B, the expression level of GM-CSF in group A was significantly increased ( P < 0.05), while no significant difference was detected between group A and group B in the expression levels of IL-37 and CRP ( P > 0.05). In conclusion, inflammatory factors IL-37, GM-CSF, CRP, and Hcy were all involved in the pathogenesis of AS, and the increased levels of GM-CSF were closely related to the progress of unstable plaques. These results may aid the early diagnosis/treatment of AS.

Differential Expression Of TREM-1 In Myelogenous Leukemia Cells

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 4951-4951 ◽  
Author(s):  
Huiyu Li ◽  
Wenying Li ◽  
Xiaoling Yi ◽  
Shiang Huang ◽  
Wei Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Complete Remission ◽  
Patient Group ◽  
Peripheral Blood ◽  
Myelogenous Leukemia ◽  
Leukemia Cells ◽  
Control Group ◽  
Healthy Controls ◽  
Significant Difference ◽  
Patient Groups

Abstract Objectives Triggering receptor expressed on myeloid cells (TREM) -1 is a receptor as a member of the immunoglobulin superfamily expressed on the cell-surface of neutrophils, monocytes and macrophages. This receptor amplifies the inflammatory response, activating the signaling pathway. TREM-1 expression is associated with mature myeloid cell development. TREM-1 is shed from the membrane of activated macrophages without the transmembrane and intracellular domains, and can be found as soluble TREM (sTREM)-1. Soluble TREM-1 is thought to negatively regulate TREM receptor signaling. Some studies currently reported that TREM-1 regulates the malignant behavior of cancer cells in lung cancer and HCC. However, no related studies about the role of TREM-1 in leukemia have been carried out. The aims of this study was investigated the TREM-1 expression in myelogenous leukemia cells. Methods Thirty-five patients with AML, twenty-five patients with CML and a control group of eleven healthy people were subjected to the study. TREM-1 expressions on the surfaces of leukemia cells were measured by flow cytometry. Plasma sTREM-1 levels were measured by ELISA. Results In this study, our results provide the first evidence that TREM-1 was differentially expressed in myelogenous leukemia cells. The TREM-1 mean ratio of median fluorescence intensity (mean ratio of MFI) was 3.13±0.88 and 2.52±0.40 in CML and AML patients, respectively. The TREM-1 mean ratio of MFI was 3.03±1.40 in myelogenous leukemia cell lines (K562, HL60, THP-1). The TREM-1 mean ratio of MFI was 5.37±0.88 in healthy controls. Compared to healthy controls, myelogenous leukemia cells had decreased TREM-1 expressions (P<0.001). The TREM-1 mean ratio of MFI was 4.89±0.60 in patients who are in complete remission after Novartis's Gleevec therapy. Compared with CML patient groups, patients who are in complete remission after Gleevec therapy had rising TREM-1 expressions (P<0.01). TREM-1 expressions of patients who are in complete remission after Gleevec therapy were slightly lower than the healthy controls, but this did not reach significance. No significant difference in TREM-1 expressions was seen between AML and CML patient groups, male and female patient groups, and cells derived from peripheral blood and bone marrow of the same leukemia patients (p>0.1). In addition, the plasma sTREM-1 levels were measured by ELISA. sTREM-1 levels was 48.54±57.63pg/mL for AML group and 43.72±23.93pg/mL for CML group. Results indicated that plasma sTREM-1 levels significantly higher in AML and CML patients than that in healthy controls (P<0.01). However, there was no significant difference in plasma sTREM-1 levels observed in AML patient group compared with CML patient group, male patients group compared with female patients group, and plasma from peripheral blood compared with plasma from bone marrow of the same leukemia patients (p>0.1). An ongoing project focuses on the relationship between the function of TREM-1 and occurrence, progression and prognosis of myelogenous leukemia, advances will be reported in time. Conclusion TREM-1 expression on leukemia cells was significantly lower in patients with AML and CML than those in healthy controls and patients in complete remission had increased TREM-1 expression. Patients with AML and CML had increased plasma soluble TREM-1. The TREM-1 expression on leukemia cells had an inverse correlation with plasma sTREM-1 level in AML and CML patients. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Clinical evaluation of the effectiveness of treatment of the hand-foot syndrome – skin toxicity of antitumor target therapy with multi-kinase inhibitors

2018 ◽  
Vol 12 (3) ◽  
pp. 140-149 ◽  
Author(s):  
Evgeniya A. Shatokhina ◽  
Larisa S. Kruglova ◽  
Oleg A. Shukhov
Keyword(s):  
Lipoic Acid ◽  
Kinase Inhibitors ◽  
Clinical Manifestations ◽  
Main Group ◽  
Betamethasone Dipropionate ◽  
Control Group ◽  
Positive Trend ◽  
Alpha Lipoic Acid ◽  
Significant Difference ◽  
Hand Foot Syndrome

Background. The effective treatment of the hand-foot syndrome is a necessary component of the complex treatment of patients receiving antitumor therapy with multikinase inhibitors. Aims. The assessment of a new effective method for the treatment of palm-plantar syndrome in patients receiving multi-kinase inhibitors. Materials and methods. There are 27 patients received antitumor treatment with multi-kinase inhibitors and had clinical manifestations of the hand-foot syndrome (HFS) for observation. The main group (14 patients) used a combination of an alpha-lipoic acid at a dose of 600 mg per day per os and an ointment containing 0.005% calcipotriol and 0.05% betamethasone dipropionate 2 times a day. The control group, included 13 patients, used a combination with a cream containing 10% urea and ointment containing 0.05% betamethasone dipropionate. Results. After a week of treatment, the regression of the skin process was observed in both groups, but it was more evident in the main group: significant differences in severity of erythema, paresthesia, pain and burning sensation, the Dermatological Life Quality Index (DLQI) were observed at this stage of therapy (p < 0.05). After 2 weeks of therapy: a more positive trend in the main group in terms of erythema, paresthesia and burning, but the most significant difference is DLQI – 17.4 in the main group and 22.8 in the control group (p < 0.0001). At the end of the 3rd week of therapy: differences with the main characteristics of erythema, desquamation, paresthesia (p < 0.0008), pain (p < 0.0001), DLQI (p < 0.0001). At the end point of the study (after 4 weeks of therapy), significant differences were found in reducing the symptoms and the main parameters for evaluating the effectiveness of DLQI in patients of the main group with a reliability of p < 0.0001. The high correlation of DLQI was found with erythema, pain, burning, paresthesia and desquamation. Conclusions. The treatment of the hand-foot plantar syndrome combined using alpha-lipoic acid inside and topical therapy in the form of an ointment containing calcipotriol and betamethasone dipropionate, can be recommended for patients receiving targeted therapy with multi-kinaseinhibitors.

scholarly journals THE STATE OF THE OPTICAL DENSITY OF THE ALVEOLAR PROCESS OF THE JAWS OF RATS IN SUPRAOCCLUSIVE RELATIONSHIPS OF INDIVIDUAL TEETH IN THE AGE ASPECT

2021 ◽  
Vol 74 (8) ◽  
pp. 1800-1803
Author(s):  
Yurii V. Lakhtin ◽  
Serhii M. Zviahin ◽  
Lidia M. Karpez
Keyword(s):  
Optical Density ◽  
Statistical Significance ◽  
Statistical Processing ◽  
The State ◽  
Wilcoxon Test ◽  
Control Group ◽  
Alveolar Process ◽  
Mineral Density ◽  
Significant Difference ◽  
Experimental Group

The aim of the study was to conduct a comparative assessment of the optical density of bone tissue of the alveolar process of the jaws of rats in supraocclusive relationships of individual teeth in the age aspect. Materials and methods: The study was performed on 60 white laboratory rats. Rats were divided into control (30 individuals) and experimental (30 individuals) groups. According to the age of rats each group was divided into 3 subgroups (10 animals): young, mature and senile. In the experimental group, the state of supraocclusion was modeled by increasing the height of the lower right second molars placing a seal 1 mm high. Experimental animals were removed from the experiment on the 15th day through decapitation. The lower jaws were skeletonized, jaw blocks were sawn in the molar area. Bone mineral density was determined using optical densitometry. The statistical processing defined the average (M) and its error (m). The statistical significance of the difference in two independent groups was performed according to nonparametric criteria (Mann-Whitney U-test and W-Wilcoxon test). Statistical processing was conducted with the help of integrated package of statistical program AtteStat 12.0.5 for MS Excel. Differences at p≤ 0.05 were considered statistically significant. Results: Statistical discrepancy is present in rats of mature and senile age, as well as between the indicators of all rats of the control and experimental groups (≤0.05). No significant difference was observed in young rats, but the indicators of the experimental group were 3.82% worse than in the control group. In senile rats, the density of the alveolar process decreased by 26.6%, in adult rats by 17.5%. Conclusions: The presence of supraocclusive relationships of individual teeth causes a decrease in bone density of the alveolar process of the jaws in rats. The greatest loss of density among age groups in elderly rats.

scholarly journals Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia

2019 ◽  
Author(s):  
Somayeh Parsa ◽  
Sedigheh Sharifzadeh ◽  
Ahmad Monabati ◽  
Noorossadat Seyyedi ◽  
Reza Ranjbaran ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Peripheral Blood ◽  
Healthy Subjects ◽  
Fold Change ◽  
Lymphocytic Leukemia ◽  
Stage I ◽  
Stage Ii ◽  
Control Group ◽  
Newly Diagnosed ◽  
Significant Difference

Background: Semaphorins play prominent roles in physiological and pathological processes such as vascular development, tumor growth and immune responses. Semaphorins have different roles in various kinds of cancers, but there is no study concerning their expression in the chronic lymphocytic leukemia (CLL). This study aimed to assess the SEMA3A, SEMA4A and SEMA4D expression in patients with CLL.  Materials and Methods: Peripheral blood specimens were collected from 30 newly-diagnosed untreated patients with CLL and 30 healthy subjects as a control group. The SEMA3A, SEMA4A and SEMA4D expression was determined by real-time PCR method.  Results: The fold change expression of SEMA3A and SEMA4D was 7.58 ± 2.66 and 3.20 ± 0.99 in patients with CLL, and was 1.01 ± 0.31 and 1.00 ± 0.27 in healthy subjects, respectively. The CLL patients expressed higher amounts of SEMA3A and SEMA4D in comparison with healthy subjects (P<0.02 and P<0.03, respectively). The fold change expression of SEMA3A in patients with stage II (11.12 ± 5.35) was also higher than patients with stage I (4.49 ± 1.61, P<0.05). No significant difference was also observed in the expression of SEMA4A and SEMA4D between patients with stage I and stage II CLL. In both CLL and control groups, the fold change expression of SEMA3A was higher in men than in women (P<0.03 and P<0.02, respectively). Conclusion: The results of the study indicated elevated expression of the SEMA3A and SEMA4D in patients with CLL. The SEMA3A expression was influenced by tumor stage and gender of participants. 

scholarly journals Increased Peripheral Blood Eosinophils may Indicate Acute Infection in Neonates

2021 ◽  
Author(s):  
Weiying Wang ◽  
Yuan Zhao ◽  
Bi-Fen Yuan
Keyword(s):  
Peripheral Blood ◽  
Medical Center ◽  
Acute Infection ◽  
White Blood Cells ◽  
Disease Stage ◽  
Control Group ◽  
Blood Eosinophil ◽  
Acute Infections ◽  
Significant Difference ◽  
Blood Eosinophils

Abstract Objective To explore the association between increased blood eosinophils and frequent pathogens due to the infections in children. Methods A total of 2353 children with acute infections admitted to Guangzhou Women and Children's Medical Center from February 1, 2019 to January 31, 2020 were enrolled in the study. 277 children without infections were comprised the control group. Children’s age, peripheral blood parameters including white blood cells, eosinophils, C-reactive protein (CRP) were recorded. In addition, infection stage and departments the patients admitted to were investigated. Results Blood eosinophil numbers negatively correlated with the age of children, whereas had no relation to disease stage. The means of eosinophil for neonates (<0.1 year)，infancy (<1year) and children >1year with acute infections were 0.67±0.40, 0.40±0.68, 0.15±0.25 *109/L compared with control group matched for age(0.44±0.20, 0.45±0.27, 0.24±0.19*109/L, P <0.001, <0.001, 0.497, respectively). Among them, the mean of eosinophil in the neonates afflicted with acute infections was significantly higher than the others compared to age-matched controls (0.63±0.60 vs 0.44±0.20, P= 0.012). Areas under the curves (AUC) were 0.81 (95% CI 0.75–0.86) for eosinophil combined with CRP and 0.68 (95% CI 0.61–0.75) for CRP alone for acute infections in neonates (P=0.02). Patients admitted in ICU had higher eosinophils than outpatients (0.46±0.60 vs 0.16±0.24, P <0.001) but had no significant difference compared with control group (0.45±0.20, P >0.99). Conclusion Increased peripheral blood eosinophils may indicate acute infections among neonates. Eosinophil combined with CRP can contribute to evaluating this population.

Identifying dietary patterns in Irish schoolchildren and their association with nutritional knowledge and markers of health before and after intervention

2020 ◽  
pp. 1-9
Author(s):  
Alison Merrotsy ◽  
Aoife L. McCarthy ◽  
Sean Lacey ◽  
Tara Coppinger
Keyword(s):  
Cardiorespiratory Fitness ◽  
Dietary Patterns ◽  
Primary Schools ◽  
Physical Activity Intervention ◽  
Control Group ◽  
Positive Trend ◽  
Nutritional Knowledge ◽  
Food Diary ◽  
Post Intervention ◽  
Significant Difference

Abstract The aim of the study was to identify dietary patterns (DP) and examine differences in anthropometric measures, blood pressure (BP), cardiorespiratory fitness and nutritional knowledge of 6- and 10-year-old children at baseline and following a nutrition and physical activity intervention, with respect to DP and treatment group. This is a longitudinal study. Food diary, nutritional knowledge questionnaire and 550-m walk/run test measured dietary intake, nutritional knowledge and cardiorespiratory fitness, respectively. BP, weight, height and waist circumference were also measured and BMI and waist-to-height ratio (WHtR) were derived. All measurements were performed at baseline and following intervention. Two primary schools (one intervention, one control) in Cork, Ireland, were selected. Participants were 6- (n 39, age 5·9 (sd 0·6) years) and 10- (n 49, age 9·8 (sd 0·5) years)-year-olds. Two DP were identified, using k-means cluster analysis, for both 6- (unhealthy and nutrient-dense) and 10-year-olds (processed and Western diet) at baseline. DP derived post-intervention were (1) plant-based and (2) processed foods for 6-year-olds and (1) nutrient-dense and (2) unhealthy for 10-year-olds. There was no statistically significant difference in DP for 6- and 10-year-olds at baseline and post-intervention (P > 0·05). Following the intervention, a multivariate ANOVA showed there were no statistically significant differences in nutritional knowledge, BMI, WHtR, cardiorespiratory fitness and BP based on DP and intervention/control group for both age groups (P > 0·05). Three out of four dietary patterns identified for 6- and 10-year-olds were unfavourable. While no statistically significant evidence of intervention impact was found on DP, a positive trend was emerging among 10-year-olds.

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