scholarly journals The influence of phychoemotional stress on white blood cells circulation and thrombodynamics in healthy volunteers

2013 ◽  
Vol 94 (5) ◽  
pp. 718-722
Author(s):  
A P Lozhkin ◽  
Yu V Chernokhvostov ◽  
V G Dvoenosov ◽  
M V Panasyuk ◽  
R I Zhdanov
Keyword(s):  
Healthy Volunteers ◽  
Immune Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Lower Percentage ◽  
Control Group ◽  
Female Group ◽  
Male Group ◽  
Psychoemotional Stress ◽  
Group Sex

Aim. To characterize the influence of psychoemotional stress on a white blood cells number in peripheral blood and clotting parameters. Methods. Lymphocytes, granulocytes, monocytes blood counts and coagulological testing were performed in fifty-one students (26 males and 25 females) during the midyear examinations. Psychological tests were used to reveal stress resistance and predisposition to stress. Results. The higher monocyte counts were found in male group under stress compared to control group. Meanwhile, female group under stress showed higher percentage of granulocytes and lower percentage of lymphocytes compare to control group. Sex differences in stationary speed of clot formation were found in stress and non-stress groups. Both sex groups showed an association of psychoemotional stress and hemostasis parameters, the differences in male group were more marked. Conclusion. The obtained data suggest that psychoemotional stress modulates immune cells and hemostasis parameters in healthy volunteers.

scholarly journals Platelet polyphosphate and SARS-Cov-2 mRNA-vaccine-induced inflammatory side-effects: a pilot study

2021 ◽  
Author(s):  
Takashi Uematsu ◽  
Atsushi Sato ◽  
Hachidai Aizawa ◽  
Tetsuhiro Tsujino ◽  
Taisuke Watanabe ◽  
...  
Keyword(s):  
Pilot Study ◽  
Side Effects ◽  
Immune Cells ◽  
Initial Dose ◽  
Side Effect ◽  
White Blood Cells ◽  
Small Sample ◽  
Continuous Treatment ◽  
Female Group ◽  
Male Group

Abstract Background: Platelets have recently been recognized as immune cells. Platelets first contact invading pathogens and then induce immune reactions in cooperation with white blood cells. Platelet polyphosphate (polyP), which is classically recognized as a thrombotic and hemostatic biomolecule, has recently attracted attention as a cytokine that modulates inflammation and is involved in intercellular communication between platelets and major immune cells. Objective: To determine the involvement of polyP in SARS-Cov-2-mRNA vaccine-induced immune responses, this pilot study examined the effects of mRNA vaccines on platelet polyP levels. Methods: Before and after vaccination (BNT162b2), blood samples were obtained from healthy, non-smoking individuals (relatively older male group, n=6 vs. younger female group, n=23), who did not have systemic diseases that required continuous treatment. Washed platelets were prepared and subjected to a fluorometric determination of platelet polyP levels using 4',6-diamidino-2-phenylindole. The side effects of vaccination were recorded as scores. Results: Compared with the male group, platelet polyP levels decreased in the relatively younger female group after the initial dose, while the side effect score increased in the female group after the second dose. Moderate correlation coefficients were observed between the reduction in polyP levels and the side effect scores or the original polyP levels. Conclusions: Despite being a pilot study using a small sample size, this study suggests the possibility that platelet polyP may suppress the side effects induced by the mRNA vaccines after the initial dose, but not the second dose, in relatively young female subjects who generally have high immune responsiveness.

scholarly journals Effects of dietary Mannan oligosaccharide (MOS) on grown, survival rate, intestinal morphology and blood cell count of the golden trevally fish (Gnathanodon specious)

2019 ◽  
Vol 19 (4A) ◽  
pp. 241-250
Author(s):  
Dang Tran Tu Tram ◽  
Nguyen Thi Nguyet Hue ◽  
Ho Son Lam ◽  
Nguyen Truong Tan Tai ◽  
Dao Thi Hong Ngoc
Keyword(s):  
Survival Rate ◽  
Blood Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Intestinal Morphology ◽  
The Other ◽  
Control Group ◽  
Blood Cell Count ◽  
Mannan Oligosaccharide ◽  
Erythrocyte Density

The golden trevally fishes (Gnathanodon specious) (2.19 ± 0.23 g) were cultured in glass tanks with density of 20 fishes/tank and they were fed supplemental diets of different MOS concentrations (0; 0.2; 0.4 and 0.6%) for 90 days. Collected data included growth rate, survival rate and some hematological characteristics of this fish. The results demonstrated that MOS supplementation did not affect growth performance, erythrocyte density and blood cell size, however the survival rate was significantly increased. On the other hand, the total number of white blood cells (BC) on the 60th day in the fish fed with MOS supplements (5.78–6.96 × 104TB/mm3) was higher than that in the control group (only 5.43 × 104TB/mm3) with the largest total leukocytes (6.96 ± 0.50 × 104TB /mm3) at 0.2% MOS (p < 0.05).

scholarly journals EFFECT OF ZINC ELEMENT AND DEXAMETHASONE ON SOME HEMATOLOGICAL BIOCHEMICAL TESTS IN RABBITS MALE

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
D. S. Dheyab
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
The Body ◽  
Control Group ◽  
Blood Collection ◽  
Biochemical Tests ◽  
Histological Changes ◽  
Spleen Tissue ◽  
Cholesterol Levels ◽  
Short Time

This study was conducted to investigate the effect of zinc in dose 15mg/kg.bw daily  taken by the mouth and dexamethasone 4mgIkg.Bw by injection for 30days on some hematological biochemical tests and some histological changes of liver spleen in male rabbits. Thirty rabbits were used that divided into 3 randomized groups (each group contain 10 male rabbits ). Control group was taken normal food and water, Zinc group that gave zinc at dose of 15mg/kg.BW/daily/oral on 1, 2, 3, 4 weeks. Dexamethasone with zinc group : Employ dexamethasone 4mg/Kg.Bw . I.M dialy for 1 and 2 weeks for experiment and at  3, 4th weeks they gave zn 15mg/lKg.Bw day/orally. Blood samples were taken from the heart directly in 2 and 4weeks to examine packed cell volume (pcv), white blood cells (WBCs), Red blood cells (RBCs) with differential Leuckcyte count.separation blood collection to plasma and examine glucose mg/dl , cholesterol mg/dl. In histological tests, rabbits were killed and separate their organs tissue from the body to examine liver and spleen. The results revealed  a decrease in level of RBCs, pcv after treatment with zinc 15, mg/Kg.Bw orally (zinc group) and increase in WBCs with differential leuckocyte count specially neutrophil cell, while biochemical tests show increase in glucose and cholesterol levels after treatment with dexamethasone 4mglkgBw. I/M seen increase in counts of RBCs , PCV, WBCs and differential lenkocyte count and decrease in glucose with cholesterol parameters, histological changes show change in liver after treatment by dexamethasone 4mglKg.Bw ,spleen tissue seen necrosis and pigmentation with hemorrhage after take dexamethasone 4mglkg in (dexamethasone + zinc group). Results also showed that zinc enhanced the immune system in at normal dose for limited time  because of its effect on other mineral such as copper and causes anemia , while the dexamethasone is a drug used for antianflammatory but for a short time.                                                                                                                           

EFFECT OF VARIOUS FORMS OF SELENIUM ON THE PRODUCTIVITY AND METABOLISM OF FATTENED YOUNG PIGS IN PRODUCTION CONDITIONS

2021 ◽  
pp. 55-65
Author(s):  
M.G. CHABAEV ◽  
◽  
E.YU. TSIS ◽  
R.V. NEKRASOV ◽  
M.I. KLEMENTEV
Keyword(s):  
Blood Cells ◽  
Average Daily Gain ◽  
White Blood Cells ◽  
Live Weight ◽  
Growing Pigs ◽  
Control Group ◽  
Protein Activity ◽  
Large White ◽  
Organic Selenium ◽  
Young Pigs

Selenium plays an important role for maintaining complex and adequate nutrition of fattened young pigs. It also has a big biological importance to maintain growth and health, as well as for biochemical and physiological processes This study presents the results of a production experiment to determine the effect of organic and inorganic forms of selenium on growth indicators, immunohematological blood parameters and slaughter qualities of fattened pigs in period I and II. Test feeding with various forms of selenium has been implemented with two groups of analogs (N = 60; n = 30), taking into account age and live weight. The experiment was conducted on castrates of large white breed, which were divided into two groups of 30 heads each following the principle of analogues (taking into account their age, live weight). The studied fattened pigs received standard complete feeds of the SK-5 and SK-6 types for a period of 105 days. Growing pigs were fed with organic selenium for 20 mg/kg as part of the feed 64 mixture per head in the first and second period of fattening, while the animals of the control group were fed with 0.3 mg/kg of the inorganic form of selenium. Including a chelated form of selenium in the complete feed of castrates at the rate established in the course of scientific and economic studies has led to 757 g of average daily gain, which is 8.1% more as compared to the control group. The concentration of red blood cells in the blood of growing pigs fed with 20 mg/kg of selenium chelate increased by 6.3%, hemoglobin – by 2.4%, and white blood cells – by 7.1% as compared to the control. Feeding organic selenium to fattened young pigs contributed to a significant increase in specific units of protein activity by 38.65% (p < 0.01), BASC – by 7.63%, as well as lysis – by 40.06% (p < 0.05), and phagocytic activity by 3.33% in blood serum as compared to the control. The half-carcasses of hogs who had received fed rations including selenium chelate fetured slaughter weight and slaughter yield, respectively, higher – by 6.4 and 0.6% as compared with the control analogues. The use of the established norm of organic selenium in the diets of fattened young pigs according to the results of production testing is economically profitable and contributes to a per-head profit of 420 rubles.

scholarly journals Effect of Pegbovigrastim on Hematological Profile of Simmental Dairy Cows during the Transition Period

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 841 ◽  
Author(s):  
Francesca Trimboli ◽  
Valeria Maria Morittu ◽  
Antonio Di Loria ◽  
Andrea Minuti ◽  
Antonella Anna Spina ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Blood Cells ◽  
Transition Period ◽  
Lower Percentage ◽  
Control Group ◽  
Volume Distribution ◽  
Polymorphonuclear Cells ◽  
Distribution Width ◽  
Hematological Profile ◽  
First Time

Pegbovigrastim is a long-acting analog of recombinant bovine granulocyte colony-stimulating factor, that promotes and increases the count and functionality of polymorphonuclear cells in dairy cows. The present study aimed to explore, for the first time in Simmental cows, the clinical and hematological effect of pegbovigrastim during the transition period (TP). Cows were randomly assigned into two groups: treated group (PEG; n = 16) received pegbovigrastim at approximately 7 days before expected parturition and within 6 h after calving, and control group (CTR; n = 16) received saline solution. Blood samples were obtained at −7, 0, 1, 3, 7, 14, 21, and 30 days relative to calving. PEG group showed white blood cells (WBC) count consistently higher compared with CTR group (p < 0.001) until to 3 weeks after calving. Neutrophils remained higher in PEG group (p < 0.001) up to three weeks after calving, compared with CTR group, with slight increment of band cells. Moreover, PEG group displayed a lower index of myeloperoxidase at 1, 3, and 7 days after calving (p < 0.01) compared with CTR. Basophils and lymphocytes showed a similar trend to those observed for neutrophils at 1 day after calving in PEG group. Finally, monocytes remained markedly elevated until 3 days after calving in PEG compared to CTR group (p < 0.001), whereas in PEG group, eosinophils population showed lower percentage values at 1 and 3 days after calving but higher values at 30 days compared with CTR group. PEG group was characterized by lower red blood cells (RBCs) count compared with CTR group (p < 0.05) and higher % of red cell volume distribution width (RDW) from week 2 and mean corpuscular volume (MCV) at 30 days after calving. In addition, the mean platelet volume (MPV) was significantly higher in PEG group at calving, 1, 3, and 7 days after calving compared with CTR group (p < 0.05). For the first time, we described the effect of pegbovigrastim in a breed not specialized exclusively in milk production as Holstein, but with dual purpose (meat and milk), evaluating the complete hematological profile in cows during the transition period. These results provide evidence on the proliferative effect of pegbovigrastim on WBC in Simmental breed highlighting its possible side effect on RBCs.

scholarly journals Effects of carprofen or meloxicam on selected haemostatic variables in miniature pigs after orthopaedic surgery

2011 ◽  
Vol 80 (4) ◽  
pp. 401-405 ◽  
Author(s):  
Petr Raušer ◽  
Leona Lexmaulová ◽  
Robert Srnec ◽  
Lucie Urbanová ◽  
Pavel Proks ◽  
...  
Keyword(s):  
Blood Cells ◽  
Bleeding Time ◽  
White Blood Cells ◽  
Intramuscular Administration ◽  
Control Group ◽  
Thrombin Time ◽  
Serum Urea ◽  
Miniature Pigs ◽  
Analgesic Effects ◽  
Biochemical Variables

The aim of the study was to detect and compare the haemostatic variables and bleeding after 7‑days administration of carprofen or meloxicam in clinically healthy miniature pigs. Twenty-one clinically healthy Göttingen miniature pigs were divided into 3 groups. Selected haemostatic variables such as platelet count, prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, serum biochemical variables such as total protein, bilirubin, urea, creatinine, alkaline phosphatase, alanine aminotransferase and gamma-glutamyltransferase and haemoglobin, haematocrit, red blood cells, white blood cells and buccal mucosal bleeding time were assessed before and 7 days after daily intramuscular administration of saline (1.5 ml per animal, control group), carprofen (2 mg·kg-1) or meloxicam (0.1 mg·kg-1). In pigs receiving carprofen or meloxicam, the thrombin time was significantly increased (p< 0.01) compared to the control group. In animals receiving carprofen, serum urea and creatinine were significantly decreased, compared to the control (p< 0.01) or meloxicam (p< 0.05) groups. In pigs receiving meloxicam, serum urea was significantly decreased (p< 0.05) compared to the control group. Significant differences were not detected in other haemostatic, biochemical variables or bleeding time compared to other groups or to the pretreatment values. Intramuscular administration of carprofen or meloxicam in healthy miniature pigs for 7 days causes sporadic, but not clinically important changes of selected haemostatic variables. Therefore, we can recommend them for perioperative use, e.g. for their analgesic effects, in orthopaedic or other surgical procedures without increased bleeding.

scholarly journals WHITE BLOOD CELLS RATIOS IN PATIENTS WITH ACUTE CORONARY SYNDROMES IN ASSOCIATION WITH HYPERTENSION AND DIABETES MELLITUS

2021 ◽  
Author(s):  
Iyad Alghzawi
Keyword(s):  
Diabetes Mellitus ◽  
Blood Cells ◽  
Plaque Rupture ◽  
Coronary Thrombosis ◽  
White Blood Cells ◽  
Control Group ◽  
Ischemic Event ◽  
Inflammatory Processes ◽  
Observational Cohort ◽  
Coronary Syndromes

Introduction. Evidence now indicates that inflammation contributes considerably to the initiation and progression of  atherosclerosis and an active inflammatory processes may trigger plaque rupture and enhance the risk of coronary thrombosis leading to a clinical ischemic event. The purpose of this study was to evaluate of white blood cells ratios in patients with ACS in association with arterial hypertension and 2 type Diabetes Mellitus. Material and Methods. In this observational cohort trial we observed of 184 patients with ACS. All patients were randomized into four groups: 1st group - 42 patients with ACS without AH or DM; 2nd group – 56 patients with ACS and previous AH; 3rd group – 42 patients with ACS and 2 type DM; and 4th group – 44 patients with ACS and AH and DM. We studied of leukocytes count and their subpopulation ratios: neutrophils to lymphocytes ratio (NLR), neutrophils to monocytes ratio (NMR), neutrophils to lymphocytes+monocytes ratio (N/LMR), lymphocytes to monocytes ratio (NMR). Results. The mean white blood cells count was significant higher in patients with ASC, compared with control group (p<0.001). In patients with ACS the elevated NMR and NLR were observed: 15.04±1.28 vs 11.09±0.43 in control group (p<0.05), and 3.34±0.20 vs 2.60±0.06 (p<0.05), respectively. No significant differences between WBC ratios were revealed in observed patients with ACS with or without AH and/or DM. Conclusion. ACS is characterized of raised NLR and NMR which could be indicators of poor prognosis.

scholarly journals The Effect of Broccoli Extract in Arsenic-Induced Experimental Poisoning on the Hematological, Biochemical, and Electrophoretic Parameters of the Liver and Kidney of Rats

2022 ◽  
Vol 2022 ◽  
pp. 1-9
Author(s):  
Mahdieh Raeeszadeh ◽  
Pouria Karimi ◽  
Nadia Khademi ◽  
Pejman Mortazavi
Keyword(s):  
Heavy Metals ◽  
Blood Cells ◽  
Serum Proteins ◽  
White Blood Cells ◽  
Kidney Tissue ◽  
Kidney Damage ◽  
Control Group ◽  
Histopathological Diagnosis ◽  
Liver And Kidney ◽  
Liver And Kidney Damage

Heavy metals such as arsenic contribute to environmental pollution that can lead to systemic effects in various body organs. Some medicinal plants such as broccoli have been shown to reduce the harmful effects of these heavy metals. The main aim of the present study is to evaluate the effects of broccoli extract on liver and kidney toxicity, considering hematological and biochemical changes. The experimental study was performed in 28 days on 32 male Wistar rats classified into four groups: the control group (C), a group receiving 5 mg/kg oral arsenic (AS), a group receiving 300 mg/kg broccoli (B), and a group receiving arsenic and broccoli combination (AS + B). Finally, blood samples were taken to evaluate the hematological and biochemical parameters of the liver and kidney, as well as serum proteins’ concentration. Liver and kidney tissue were fixed and stained by H&E and used for histopathological diagnosis. The results demonstrated a significant decrease in white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb) in the AS group compared to other groups. However, in the B group, a significant increase in RBC and WBC was observed compared to the AS and C groups ( P  < 0.05). Moreover, RBC and WBC levels increased significantly in the AS + B group compared to the AS group ( P  = 0.046). However, in the AS group, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine levels increased, while total protein, albumin, and globulin decreased. This can be a result of liver and kidney damage, which was observed in the AS group. Furthermore, the increase in the concentration of albumin and globulin in the AS + B group was higher than that in the AS group. Infiltration of inflammatory cells and necrosis of the liver and kidney tissue in the pathological evaluation of the AS group were significantly higher than other groups. There was an increase in superoxide dismutases (SOD), glutathione peroxidase (GPx), and total antioxidant capacity (TAC); however, a decrease in malondialdehyde (MDA) concentration was seen in the AS + B group compared to the AS group. It seems that broccoli is highly effective at reducing liver and kidney damage and improving the hematological and biochemical factors in arsenic poisoning conditions.

scholarly journals Detection of Benzo[a]Pyrene Diol Epoxide-DNA Adducts in White Blood Cells of Asphalt Plant Workers in Syria

2021 ◽  
Author(s):  
Razan Zohairee
Keyword(s):  
Dna Adducts ◽  
Blood Cells ◽  
White Blood Cells ◽  
Control Group ◽  
Study Group ◽  
Carcinogenic Potential ◽  
Potential Biomarker ◽  
Significant Difference ◽  
Polycyclic Aromatic ◽  
Peripheral White Blood Cells

Benzo[a]pyrene (B[a]P) is a major polycyclic aromatic hydrocarbon (PAH), it can bind the DNA to produce DNA-adducts, which has major carcinogenic potential. Enzyme-linked immunosorbet assay (ELISA) is the method used to detect these DNA adducts of B[a] P diolepoxide (BPDE) within the living cells.The aim of this study is to evaluate exposure to bitumen fumes, and to B[a]P in asphalt plant workers by measuring the BPDE-DNA adducts in their peripheral white blood cells (WBC), which are considered biological markers for exposure risk assessment.In this study, Hemostatic blood (CBC, AST) were measured, and the levels of BPDE-DNA adducts were measured in DNA samples of WBC obtained from asphalt plant workers in Syria and compared to those measured from a control group. The measurement was performed using BPDE-DNA Adducts ELISA.kit. The sample size was determined to be 50 with 25 asphalt plant workers and 25 healthy volunteers with no occupational exposure to PAHs.The results showed some diseases associated with exposure to asphalt fumes among the workers in the study group and a statistically significant difference in the values of (CBC; WBC, leukocytes, HCT, MCHC and AST) between the study group and the control group. BPDE-DNA adducts were detected in WBC of 11 asphalt plant workers with concentrations ranging between 0 and 2.75 ng/ml and only one individual in the control group with concentration of 0.75 ng/ml. These results indicate significant positive relationship between exposure to the bitumen fumes and formation of BPDE-DNA adducts.BPDE-DNA adducts is potential biomarker for PAHs exposure and likely helpful indicator of PAH-induced DNA damage and possibly carcinogenesis.

scholarly journals Novel predictor of the occurrence of DKA in T1DM patients without infection: A combination of neutrophil/lymphocyte ratio and white blood cells

2021 ◽  
Vol 16 (1) ◽  
pp. 1365-1376
Author(s):  
Yiping Cheng ◽  
Wenhao Yu ◽  
Yuping Zhou ◽  
Tao Zhang ◽  
Haiyan Chi ◽  
...  
Keyword(s):  
Blood Cells ◽  
Low Cost ◽  
Characteristic Curve ◽  
White Blood Cells ◽  
Area Under The Curve ◽  
Control Group ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio

Abstract The role of inflammation has been identified in the pathogenesis of diabetic ketoacidosis (DKA). The neutrophil/lymphocyte ratio (NLR) and white blood cells (WBC) can be used to predict a systemic inflammatory response. Changes in NLR and WBC levels have never been explored in type 1 diabetes mellitus (T1DM) patients with DKA and an uninfected state. This retrospective study included a total of 644 participants. NLR and WBC were measured in the control group (n = 316) and in T1DM patients with mild-DKA (n = 92), severe-DKA (n = 52), and non-DKA (n = 184) in an uninfected state. Then, we assessed the independent predictors of DKA occurrence in T1DM patients in an uninfected state. The diagnostic performance of variables was determined by receiver operating characteristic curve analysis. Serum NLR of T1DM patients is significantly higher than that of normal controls, and if DKA occurs, NLR increases further and increases with the severity of DKA. In addition to diastolic blood pressure, blood urea nitrogen, glycated hemoglobin (HbA1c), and WBC, NLR was also independently associated with DKA in T1DM patients with an uninfected state (OR = 1.386, 95% CI: 1.127–1.705, p = 0.002). Furthermore, the diagnosis analysis showed that except for NLR and WBC, the area under the curve (AUC) of indicators with a statistical difference in patients with and without DKA were 0.747 for DKA diagnosis, and after the addition of NLR and WBC, the AUC was 0.806. The increased NLR level represents a low-cost and highly accessible predictor for DKA in T1DM patients with an uninfected state. The addition of inflammation indicators can play a statistically significant role in the prediction model of the DKA occurrence.

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