Effects of carprofen or meloxicam on selected haemostatic variables in miniature pigs after orthopaedic surgery

The aim of the study was to detect and compare the haemostatic variables and bleeding after 7‑days administration of carprofen or meloxicam in clinically healthy miniature pigs. Twenty-one clinically healthy Göttingen miniature pigs were divided into 3 groups. Selected haemostatic variables such as platelet count, prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, serum biochemical variables such as total protein, bilirubin, urea, creatinine, alkaline phosphatase, alanine aminotransferase and gamma-glutamyltransferase and haemoglobin, haematocrit, red blood cells, white blood cells and buccal mucosal bleeding time were assessed before and 7 days after daily intramuscular administration of saline (1.5 ml per animal, control group), carprofen (2 mg·kg-1) or meloxicam (0.1 mg·kg-1). In pigs receiving carprofen or meloxicam, the thrombin time was significantly increased (p< 0.01) compared to the control group. In animals receiving carprofen, serum urea and creatinine were significantly decreased, compared to the control (p< 0.01) or meloxicam (p< 0.05) groups. In pigs receiving meloxicam, serum urea was significantly decreased (p< 0.05) compared to the control group. Significant differences were not detected in other haemostatic, biochemical variables or bleeding time compared to other groups or to the pretreatment values. Intramuscular administration of carprofen or meloxicam in healthy miniature pigs for 7 days causes sporadic, but not clinically important changes of selected haemostatic variables. Therefore, we can recommend them for perioperative use, e.g. for their analgesic effects, in orthopaedic or other surgical procedures without increased bleeding.

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Effects of dietary Mannan oligosaccharide (MOS) on grown, survival rate, intestinal morphology and blood cell count of the golden trevally fish (Gnathanodon specious)

Tạp chí Khoa học và Công nghệ Biển ◽

10.15625/1859-3097/19/4a/14600 ◽

2019 ◽

Vol 19 (4A) ◽

pp. 241-250

Author(s):

Dang Tran Tu Tram ◽

Nguyen Thi Nguyet Hue ◽

Ho Son Lam ◽

Nguyen Truong Tan Tai ◽

Dao Thi Hong Ngoc

Keyword(s):

Survival Rate ◽

Blood Cell ◽

Blood Cells ◽

White Blood Cells ◽

Intestinal Morphology ◽

The Other ◽

Control Group ◽

Blood Cell Count ◽

Mannan Oligosaccharide ◽

Erythrocyte Density

The golden trevally fishes (Gnathanodon specious) (2.19 ± 0.23 g) were cultured in glass tanks with density of 20 fishes/tank and they were fed supplemental diets of different MOS concentrations (0; 0.2; 0.4 and 0.6%) for 90 days. Collected data included growth rate, survival rate and some hematological characteristics of this fish. The results demonstrated that MOS supplementation did not affect growth performance, erythrocyte density and blood cell size, however the survival rate was significantly increased. On the other hand, the total number of white blood cells (BC) on the 60th day in the fish fed with MOS supplements (5.78–6.96 × 104TB/mm3) was higher than that in the control group (only 5.43 × 104TB/mm3) with the largest total leukocytes (6.96 ± 0.50 × 104TB /mm3) at 0.2% MOS (p < 0.05).

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EFFECT OF ZINC ELEMENT AND DEXAMETHASONE ON SOME HEMATOLOGICAL BIOCHEMICAL TESTS IN RABBITS MALE

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v47i6.484 ◽

2016 ◽

Vol 47 (6) ◽

Author(s):

D. S. Dheyab

Keyword(s):

Blood Cells ◽

White Blood Cells ◽

The Body ◽

Control Group ◽

Blood Collection ◽

Biochemical Tests ◽

Histological Changes ◽

Spleen Tissue ◽

Cholesterol Levels ◽

Short Time

This study was conducted to investigate the effect of zinc in dose 15mg/kg.bw daily taken by the mouth and dexamethasone 4mgIkg.Bw by injection for 30days on some hematological biochemical tests and some histological changes of liver spleen in male rabbits. Thirty rabbits were used that divided into 3 randomized groups (each group contain 10 male rabbits ). Control group was taken normal food and water, Zinc group that gave zinc at dose of 15mg/kg.BW/daily/oral on 1, 2, 3, 4 weeks. Dexamethasone with zinc group : Employ dexamethasone 4mg/Kg.Bw . I.M dialy for 1 and 2 weeks for experiment and at 3, 4th weeks they gave zn 15mg/lKg.Bw day/orally. Blood samples were taken from the heart directly in 2 and 4weeks to examine packed cell volume (pcv), white blood cells (WBCs), Red blood cells (RBCs) with differential Leuckcyte count.separation blood collection to plasma and examine glucose mg/dl , cholesterol mg/dl. In histological tests, rabbits were killed and separate their organs tissue from the body to examine liver and spleen. The results revealed a decrease in level of RBCs, pcv after treatment with zinc 15, mg/Kg.Bw orally (zinc group) and increase in WBCs with differential leuckocyte count specially neutrophil cell, while biochemical tests show increase in glucose and cholesterol levels after treatment with dexamethasone 4mglkgBw. I/M seen increase in counts of RBCs , PCV, WBCs and differential lenkocyte count and decrease in glucose with cholesterol parameters, histological changes show change in liver after treatment by dexamethasone 4mglKg.Bw ,spleen tissue seen necrosis and pigmentation with hemorrhage after take dexamethasone 4mglkg in (dexamethasone + zinc group). Results also showed that zinc enhanced the immune system in at normal dose for limited time because of its effect on other mineral such as copper and causes anemia , while the dexamethasone is a drug used for antianflammatory but for a short time.

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EFFECT OF VARIOUS FORMS OF SELENIUM ON THE PRODUCTIVITY AND METABOLISM OF FATTENED YOUNG PIGS IN PRODUCTION CONDITIONS

Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii ◽

10.26897/0021-342x-2021-1-55-65 ◽

2021 ◽

pp. 55-65

Author(s):

M.G. CHABAEV ◽

◽

E.YU. TSIS ◽

R.V. NEKRASOV ◽

M.I. KLEMENTEV

Keyword(s):

Blood Cells ◽

Average Daily Gain ◽

White Blood Cells ◽

Live Weight ◽

Growing Pigs ◽

Control Group ◽

Protein Activity ◽

Large White ◽

Organic Selenium ◽

Young Pigs

Selenium plays an important role for maintaining complex and adequate nutrition of fattened young pigs. It also has a big biological importance to maintain growth and health, as well as for biochemical and physiological processes This study presents the results of a production experiment to determine the effect of organic and inorganic forms of selenium on growth indicators, immunohematological blood parameters and slaughter qualities of fattened pigs in period I and II. Test feeding with various forms of selenium has been implemented with two groups of analogs (N = 60; n = 30), taking into account age and live weight. The experiment was conducted on castrates of large white breed, which were divided into two groups of 30 heads each following the principle of analogues (taking into account their age, live weight). The studied fattened pigs received standard complete feeds of the SK-5 and SK-6 types for a period of 105 days. Growing pigs were fed with organic selenium for 20 mg/kg as part of the feed 64 mixture per head in the first and second period of fattening, while the animals of the control group were fed with 0.3 mg/kg of the inorganic form of selenium. Including a chelated form of selenium in the complete feed of castrates at the rate established in the course of scientific and economic studies has led to 757 g of average daily gain, which is 8.1% more as compared to the control group. The concentration of red blood cells in the blood of growing pigs fed with 20 mg/kg of selenium chelate increased by 6.3%, hemoglobin – by 2.4%, and white blood cells – by 7.1% as compared to the control. Feeding organic selenium to fattened young pigs contributed to a significant increase in specific units of protein activity by 38.65% (p < 0.01), BASC – by 7.63%, as well as lysis – by 40.06% (p < 0.05), and phagocytic activity by 3.33% in blood serum as compared to the control. The half-carcasses of hogs who had received fed rations including selenium chelate fetured slaughter weight and slaughter yield, respectively, higher – by 6.4 and 0.6% as compared with the control analogues. The use of the established norm of organic selenium in the diets of fattened young pigs according to the results of production testing is economically profitable and contributes to a per-head profit of 420 rubles.

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WHITE BLOOD CELLS RATIOS IN PATIENTS WITH ACUTE CORONARY SYNDROMES IN ASSOCIATION WITH HYPERTENSION AND DIABETES MELLITUS

Science Review ◽

10.31435/rsglobal_sr/30042021/7535 ◽

2021 ◽

Author(s):

Iyad Alghzawi

Keyword(s):

Diabetes Mellitus ◽

Blood Cells ◽

Plaque Rupture ◽

Coronary Thrombosis ◽

White Blood Cells ◽

Control Group ◽

Ischemic Event ◽

Inflammatory Processes ◽

Observational Cohort ◽

Coronary Syndromes

Introduction. Evidence now indicates that inflammation contributes considerably to the initiation and progression of atherosclerosis and an active inflammatory processes may trigger plaque rupture and enhance the risk of coronary thrombosis leading to a clinical ischemic event. The purpose of this study was to evaluate of white blood cells ratios in patients with ACS in association with arterial hypertension and 2 type Diabetes Mellitus. Material and Methods. In this observational cohort trial we observed of 184 patients with ACS. All patients were randomized into four groups: 1st group - 42 patients with ACS without AH or DM; 2nd group – 56 patients with ACS and previous AH; 3rd group – 42 patients with ACS and 2 type DM; and 4th group – 44 patients with ACS and AH and DM. We studied of leukocytes count and their subpopulation ratios: neutrophils to lymphocytes ratio (NLR), neutrophils to monocytes ratio (NMR), neutrophils to lymphocytes+monocytes ratio (N/LMR), lymphocytes to monocytes ratio (NMR). Results. The mean white blood cells count was significant higher in patients with ASC, compared with control group (p<0.001). In patients with ACS the elevated NMR and NLR were observed: 15.04±1.28 vs 11.09±0.43 in control group (p<0.05), and 3.34±0.20 vs 2.60±0.06 (p<0.05), respectively. No significant differences between WBC ratios were revealed in observed patients with ACS with or without AH and/or DM. Conclusion. ACS is characterized of raised NLR and NMR which could be indicators of poor prognosis.

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The Effect of Broccoli Extract in Arsenic-Induced Experimental Poisoning on the Hematological, Biochemical, and Electrophoretic Parameters of the Liver and Kidney of Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2022/3509706 ◽

2022 ◽

Vol 2022 ◽

pp. 1-9

Author(s):

Mahdieh Raeeszadeh ◽

Pouria Karimi ◽

Nadia Khademi ◽

Pejman Mortazavi

Keyword(s):

Heavy Metals ◽

Blood Cells ◽

Serum Proteins ◽

White Blood Cells ◽

Kidney Tissue ◽

Kidney Damage ◽

Control Group ◽

Histopathological Diagnosis ◽

Liver And Kidney ◽

Liver And Kidney Damage

Heavy metals such as arsenic contribute to environmental pollution that can lead to systemic effects in various body organs. Some medicinal plants such as broccoli have been shown to reduce the harmful effects of these heavy metals. The main aim of the present study is to evaluate the effects of broccoli extract on liver and kidney toxicity, considering hematological and biochemical changes. The experimental study was performed in 28 days on 32 male Wistar rats classified into four groups: the control group (C), a group receiving 5 mg/kg oral arsenic (AS), a group receiving 300 mg/kg broccoli (B), and a group receiving arsenic and broccoli combination (AS + B). Finally, blood samples were taken to evaluate the hematological and biochemical parameters of the liver and kidney, as well as serum proteins’ concentration. Liver and kidney tissue were fixed and stained by H&E and used for histopathological diagnosis. The results demonstrated a significant decrease in white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb) in the AS group compared to other groups. However, in the B group, a significant increase in RBC and WBC was observed compared to the AS and C groups ( P < 0.05). Moreover, RBC and WBC levels increased significantly in the AS + B group compared to the AS group ( P = 0.046). However, in the AS group, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine levels increased, while total protein, albumin, and globulin decreased. This can be a result of liver and kidney damage, which was observed in the AS group. Furthermore, the increase in the concentration of albumin and globulin in the AS + B group was higher than that in the AS group. Infiltration of inflammatory cells and necrosis of the liver and kidney tissue in the pathological evaluation of the AS group were significantly higher than other groups. There was an increase in superoxide dismutases (SOD), glutathione peroxidase (GPx), and total antioxidant capacity (TAC); however, a decrease in malondialdehyde (MDA) concentration was seen in the AS + B group compared to the AS group. It seems that broccoli is highly effective at reducing liver and kidney damage and improving the hematological and biochemical factors in arsenic poisoning conditions.

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Detection of Benzo[a]Pyrene Diol Epoxide-DNA Adducts in White Blood Cells of Asphalt Plant Workers in Syria

10.5772/intechopen.96437 ◽

2021 ◽

Author(s):

Razan Zohairee

Keyword(s):

Dna Adducts ◽

Blood Cells ◽

White Blood Cells ◽

Control Group ◽

Study Group ◽

Carcinogenic Potential ◽

Potential Biomarker ◽

Significant Difference ◽

Polycyclic Aromatic ◽

Peripheral White Blood Cells

Benzo[a]pyrene (B[a]P) is a major polycyclic aromatic hydrocarbon (PAH), it can bind the DNA to produce DNA-adducts, which has major carcinogenic potential. Enzyme-linked immunosorbet assay (ELISA) is the method used to detect these DNA adducts of B[a] P diolepoxide (BPDE) within the living cells.The aim of this study is to evaluate exposure to bitumen fumes, and to B[a]P in asphalt plant workers by measuring the BPDE-DNA adducts in their peripheral white blood cells (WBC), which are considered biological markers for exposure risk assessment.In this study, Hemostatic blood (CBC, AST) were measured, and the levels of BPDE-DNA adducts were measured in DNA samples of WBC obtained from asphalt plant workers in Syria and compared to those measured from a control group. The measurement was performed using BPDE-DNA Adducts ELISA.kit. The sample size was determined to be 50 with 25 asphalt plant workers and 25 healthy volunteers with no occupational exposure to PAHs.The results showed some diseases associated with exposure to asphalt fumes among the workers in the study group and a statistically significant difference in the values of (CBC; WBC, leukocytes, HCT, MCHC and AST) between the study group and the control group. BPDE-DNA adducts were detected in WBC of 11 asphalt plant workers with concentrations ranging between 0 and 2.75 ng/ml and only one individual in the control group with concentration of 0.75 ng/ml. These results indicate significant positive relationship between exposure to the bitumen fumes and formation of BPDE-DNA adducts.BPDE-DNA adducts is potential biomarker for PAHs exposure and likely helpful indicator of PAH-induced DNA damage and possibly carcinogenesis.

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Novel predictor of the occurrence of DKA in T1DM patients without infection: A combination of neutrophil/lymphocyte ratio and white blood cells

Open Life Sciences ◽

10.1515/biol-2021-0141 ◽

2021 ◽

Vol 16 (1) ◽

pp. 1365-1376

Author(s):

Yiping Cheng ◽

Wenhao Yu ◽

Yuping Zhou ◽

Tao Zhang ◽

Haiyan Chi ◽

...

Keyword(s):

Blood Cells ◽

Low Cost ◽

Characteristic Curve ◽

White Blood Cells ◽

Area Under The Curve ◽

Control Group ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio

Abstract The role of inflammation has been identified in the pathogenesis of diabetic ketoacidosis (DKA). The neutrophil/lymphocyte ratio (NLR) and white blood cells (WBC) can be used to predict a systemic inflammatory response. Changes in NLR and WBC levels have never been explored in type 1 diabetes mellitus (T1DM) patients with DKA and an uninfected state. This retrospective study included a total of 644 participants. NLR and WBC were measured in the control group (n = 316) and in T1DM patients with mild-DKA (n = 92), severe-DKA (n = 52), and non-DKA (n = 184) in an uninfected state. Then, we assessed the independent predictors of DKA occurrence in T1DM patients in an uninfected state. The diagnostic performance of variables was determined by receiver operating characteristic curve analysis. Serum NLR of T1DM patients is significantly higher than that of normal controls, and if DKA occurs, NLR increases further and increases with the severity of DKA. In addition to diastolic blood pressure, blood urea nitrogen, glycated hemoglobin (HbA1c), and WBC, NLR was also independently associated with DKA in T1DM patients with an uninfected state (OR = 1.386, 95% CI: 1.127–1.705, p = 0.002). Furthermore, the diagnosis analysis showed that except for NLR and WBC, the area under the curve (AUC) of indicators with a statistical difference in patients with and without DKA were 0.747 for DKA diagnosis, and after the addition of NLR and WBC, the AUC was 0.806. The increased NLR level represents a low-cost and highly accessible predictor for DKA in T1DM patients with an uninfected state. The addition of inflammation indicators can play a statistically significant role in the prediction model of the DKA occurrence.

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Features of interleukin-19 production in patients with atherosclerosis

The Siberian Journal of Clinical and Experimental Medicine ◽

10.29001/2073-8552-2021-36-2-52-60 ◽

2021 ◽

Vol 36 (2) ◽

pp. 52-60

Author(s):

J. V. Saranchina ◽

S. V. Dutova ◽

O. Y. Kilina ◽

N. V. Khanarin ◽

T. S. Kulakova

Keyword(s):

Atherosclerotic Plaque ◽

Blood Cells ◽

Venous Blood ◽

White Blood Cells ◽

Fold Increase ◽

Serum Levels ◽

Control Group ◽

Atherosclerotic Plaques ◽

Blood Leukocytes ◽

Spontaneous Expression

Objective. To study the features of local and systemic production of interleukin-19 in patients with atherosclerosis.Material and Methods. The study comprised a total of 46 patients (26 women and 20 men) treated for arterial hypertension in the therapeutic department of Republican Clinical Hospital named after G.Y. Remishevskaya. The mean age of subjects was 63.4 ± 3.2 years. The control group included 40 patients (23 women and 17 men aged 44.7 ± 5.5 years) who did not have atherosclerosis. Samples of atherosclerotic plaques and venous blood were examined. Atherosclerotic plaques were obtained by endarterectomy and then subjected to homogenization followed by enzymatic hydrolysis for 1 h at 37 °C with collagenase IV in the presence of proteinase III inhibitors. The serum levels of cytokines (in the control group and in patients with atherosclerosis) and in the atherosclerotic plaque homogenate (in patients with atherosclerosis) were determined by ELISA. To assess the cytokine-producing capacity of blood leukocytes and white blood cells isolated from atherosclerotic plaques, spontaneous and phytohemagglutinin (PHA)-induced cytokine production was determined when the cells were cultured in RPMI-1640.Results. The serum levels of IL-19 did not significantly differ between the patients with atherosclerosis and the control group. A statistically significant two-fold increase in the spontaneous expression of IL-19 by blood leukocytes was observed in the group of patients with atherosclerosis in comparison with the control group. When comparing the contents of IL-19 in blood serum and atherosclerotic plaque homogenate in patients with atherosclerosis, no statistically significant differences were found (p = 0.182). The level of PHA-induced IL-19 production by the atherosclerotic plaque white blood cells was significantly lower than that of blood leucocytes.Conclusion. The study showed that the reserve capacity for IL-19 synthesis in the atherosclerotic plaque white blood cells decreases leading to the progression of inflammation. The obtained results suggest that IL-19 plays the anti-atherogenic role and its production is involved in the maintaining the mechanisms for down-regulation of inflammation in atherosclerotic plaques.

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Effect of chamomile on chemotherapy-induced neutropenia: a pilot open-label controlled trial

10.53350/pjmhs211571966 ◽

2021 ◽

Vol 15 (7) ◽

pp. 1966-1968

Author(s):

Mahdi Shahriari ◽

Mohammad Azadbakht ◽

Maryam Roohparvar ◽

Babak Daneshfard ◽

Majid Nimrouzi

Keyword(s):

Blood Cells ◽

Controlled Trial ◽

White Blood Cells ◽

Control Group ◽

Common Complication ◽

Healthy Individuals ◽

Open Label ◽

Herbal Formulation ◽

Neutropenic Patients ◽

Chemotherapy Induced Neutropenia

Neutropenia is a common complication of chemotherapy in leukemic patients. An herbal formulation of chamomile was hypothesized to be effective on neutropenia. A group of healthy volunteers and two groups of patients received chamomile oral drop to be compared with a control group of neutropenic patients. Results showed an increase of white blood cells and resolution of neutropenia in all groups except for the control group. In conclusion, chamomile could be used as an effective complementary medicine for increasing the immunity of neutropenic patients (in addition to healthy individuals). Keywords: Chamomile; Leukemia; Chemotherapy; Neutropenia; Integrative Medicine; Persian Medicine

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Physiological status of broiler chickens with diets supplemented with milk thistle extract

Veterinary World ◽

10.14202/vetworld.2021.1319-1323 ◽

2021 ◽

pp. 1319-1323

Author(s):

Olga Bagno ◽

Sergey Shevchenko ◽

Antonina Shevchenko ◽

Oleg Prokhorov ◽

Anna Shentseva ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Broiler Chickens ◽

Clinical Chemistry ◽

Current Trend ◽

White Blood Cells ◽

Control Group ◽

Physiological Status ◽

Milk Thistle ◽

Wing Vein

Background and Aim: In recent decades, the use of various feed supplements is the current trend in poultry farming, among which phytogenics serve as alternatives to feed antibiotics. This study aimed to examine the effect of feeding various doses of milk thistle extract (Silybum marianum) on the morphological and biochemical parameters of the blood in broiler chickens. Materials and Methods: Experiments were carried out in an industrial poultry farm on broiler chickens of the Hubbard ISA F15 cross for 40 days. One control group and five experimental groups of day-old chickens were formed. The number of birds in each group was 50. Broilers of all groups received complete feed, and the experimental groups received an additional milk thistle extract at doses of 0.1, 0.5, 1.0, 1.5, and 2.0 mg/kg of body weight. Milk thistle medicinal plant extract was obtained using water-ethanol extraction followed by low-temperature vacuum drying. For the assessment of blood analyses, samples were collected from the wing vein of six chickens per group. Using unified methods recommended by the International Federation of Clinical Chemistry, the content of red blood cells, hemoglobin, white blood cells, total protein, protein fractions, triglycerides, glucose, calcium, phosphorus, and the concentration of alanine aminotransferase and aspartate aminotransferase in the blood serum were determined. Results: It was found that the introduction of milk thistle extract into the diet of broiler chickens with the aforementioned doses increased the number of red blood cells, hemoglobin, white blood cells in the blood, as well as a decrease in the level of albumin and an increase in the content of γ-globulins in its serum. Conclusion: The authors assume that the introduction of milk thistle extract into a complete feed for broiler chickens increased the anabolic processes in their bodies, accompanied by increased use of proteins of the albumin fraction as the main material for organogenesis.

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