scholarly journals Morphometry of Male Reproductive Organ and Sperm Evaluation of Belgian Blue Crossbreed Cattle as a Candidate of Superior Bull

2020 ◽  
Vol 44 (4) ◽  
Author(s):  
Riyan Nugroho Aji ◽  
Panjono Panjono ◽  
Ali Agus ◽  
Budi Prasetyo Widyobroto ◽  
Tety Hartatik ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Descriptive Analysis ◽  
Reproductive Organs ◽  
Reproductive Organ ◽  
Genital Organ ◽  
General Physical Examination ◽  
Frozen Semen ◽  
General Physical ◽  
Ib Method

The purpose of this study was to observe the size of reproductive organ and to evaluate the sperm quality of Belgian Blue (BB) crossbreed cattle. This cattle are the crossbreeding result between a female Brahman Cross (BX) cattle inseminated with frozen semen from pure male BB cattle, by Artificial Insemination (IB) method, as a candidate of superior bull. The samples of this study were 2.5 years old (BB1), (BB2), and (BB3) crossbreed cattle. Its process involved general physical examination, genital organ inspection, sperm production, and quality assessment. The data obtained were analyzed through descriptive analysis. The results of this study were the three BB crossbred cattle showed the good appearance of normal reproductive organs with scrotum circumferences of each bull were 40.2 cm, 38.6 cm, and 39.8 cm respectively, and sperm quality of the good and the normal motility values of each bull were 82%, 81% and 80%, viability 84%, 83%, and 86%, and the sperm concentrations of each bull were 2.218 million/ml, 1.986 million/ml and 2.120 million/ml respectively. As a result, it could be concluded that those three BB crossbreed cattle are suitable for being used as a superior bull.

scholarly journals Efektivitas Penambahan Vitamin E (alfa-Tokoferol) dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman

2012 ◽  
Vol 12 (2) ◽  
pp. 7-13
Author(s):  
Dasrul Dasrul ◽  
Rasmaidar Rasmaidar ◽  
Abdul Harris
Keyword(s):  
Vitamin E ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Average Percentage ◽  
Treatment Groups ◽  
Frozen Semen ◽  
Brahman Cattle ◽  
Sperm Membrane ◽  
Better Than

Effect of vitamin E addition (alfa-tokoferol) into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoa ABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0), 0.1gr /100 ml medium (K1); 0.2gr/100 ml medium (K2) and 0.3 g / 100 ml medium (K4), each group was repeated 5 times. Examination of motility, viability and integrity of sperm membrane performed according to WHO standards. The data obtained were analyzed with one-way ANOVA and Duncan test. The average percentage of motility, viability and membrane integrity of spermatozoa in the addition of vitamin E were significantly different (P 0.05) compared to the control. Percentage of motility, viability and membrane integrity of spermatozoa in the group K2 significantly higher (P 0.05) compared with the group K3: K1 and K0. Percentage of motility, viability and sperm capacitation and sperm live on group K3 significantly higher (P 0.05) compared with the K1 and K0. While the percentage motility of spermatozoa in the group K1 higher were not significant (P 0.05) compared with the group K0. The addition of vitamin E in the medium on the process of washing spermatozoa Brahman cattle. The addition of vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in maintaining the percentage of motility and live spermatozoa Brahman cattle.

scholarly journals PENGARUH LAMA THAWING TERHADAP KUALITAS SPERMATOZOA SEMEN BEKU SAPI BALI (Bos Sondaicus) di DINAS PERTANIAN PROVINSI MALUKU UTARA (Effect of Thawing Time on the Quality of Frozen Semen Spermatozoa of Bali Cattle (Bos Sondaicus) In Agricultural Department of North Maluku)

2019 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Fifi Andrefani ◽  
Oktora Dwi Putranti ◽  
Abdurahman Hoda
Keyword(s):  
Analysis Of Variance ◽  
Sperm Quality ◽  
Tap Water ◽  
Semen Analysis ◽  
Best Value ◽  
Frozen Semen ◽  
Bali Cattle

Abstrak Penelitian ini dilaksanakan di Dinas Pertanian Provinsi Maluku Utara (Sofifi). Tujuan dari penelitian ini untuk mengetahui pengaruh lama thawing terhadap kualitas semen beku Sapi Bali. Analisis data yang digunakan dalam penelitian ini menggunakan Anlisis Of Variance (ANOVA) satu arah dengan empat macam perlakuan yaitu 15, 30, 45 dan 50 detik menggunakan air ledeng (PDAM) temperature 25‐30oC dan dilakukan 4 kali ulangan. Perlakuan pada penelitian ini yaitu P1: thawing 15 detik, P2: thawing 30 detik, P3: thawing 45 detik, dan P4: thawing 50 detik. Parameter yang diamati pada penelitian ini adalah Motilitas, Viatabilitas, dan Abnormalitas. Hasil menunjukkan nilai terbaik Motilitas pada 15 detik sebesar 86,25%. Viatabilitas sebesar 83,75%. Sedangkan pada Abnormalitas thawing 15 detik memiliki nilai yang lebih rendah sebesar 15,00%. Berdasarkan hasil penelitian dapat disimpulkan bahwa lama thawing 15 detik dan 30 detik mempunyai kualitas sperma yang lebih baik daripada lama thawing 45 dan 50 detik. Kata Kunci : Thawing, Motilitas, Viatabilitas, dan Abnormalitas. Abstract This research was carried out in the agricultural department of North Maluku Province (Sofifi). The purpose of this study was to determine the effect of thawing time on the quality of Bali frozen semen. Analysis of the data used in this study used an one-way analysis of variance (ANOVA) with four types of treatments, namely 15 seconds, 30,45, and 50 seconds using tap water (PDAM) temperature of 25-30°C and carried out 4 replications. This research is: P1: 15 seconds thawing, P2: 30 seconds thawing, P3: 45 seconds thawing, and P4: 50 seconds thawing. The parameters observed in this study were motility, viatability, and abnormalities. The results showed the best value of motility at 15 seconds at 86.25%. Viatability is 83.75%. Whereas the 15 second thawing abnormality has a lower value of 15.00%. Based on the results of the study it can be concluded that the length of thawing 15 seconds and 30 seconds has better sperm quality than the thawing duration of 45 and 50 seconds. Keywords : Thawing, Motility, Viatability, and Abnormalities.

scholarly journals Pengaruh Metode Thawing terhadap Kualitas Semen Beku Sapi Bali, Sapi Madura dan Sapi PO

2012 ◽  
Vol 12 (2) ◽  
pp. 14-19
Author(s):  
Muhammad Ade Salim ◽  
Trinil Susilawati ◽  
Sri Wahyuningsih
Keyword(s):  
Sperm Quality ◽  
Block Design ◽  
Bovine Semen ◽  
Bovine Sperm ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Temperature Water

Effect of thawing technique to quality frozen semen spermatozoa in bali, madura and PO cattleABSTRACT. The experiment was conducted from November 2011 to January 2012 in BBIB Singosari. The study aims was to determine the effect of thawing techniques on the quality frozen bovine sperm Bali, Madura and PO cattle. This material used 90 frozen straw of three cattle from the three breed. The method is an experiment with three treatments and 10 replications for frozen to thawing in temperature water is 5 0C of duration 60 seconds, 15 0C duration 30 seconds and 37 0C duration 15 seconds. Variables observed in this study were sperm quality are motility, viability and abnormality. Data in percentage transformed in to arcus sinus were analyzed using Completely Randomized Block Design (CRBD) with replication as blocks of 10x. The results showed the thawing at 37 0C duration 15 seconds to highly significant effect (P0,01) viability and motility and significant effect (P0.05). The abnormalities, no significant effect (P0,05). Breed cattle not significant effect to quality. It can be concluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle. Thawing using 37 0C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm.

Pengaruh Lama Thawing yang Berbeda pada Suhu 25 oC Terhadap Kualitas Semen Beku Sapi Ongole

2019 ◽  
Vol 6 (1) ◽  
pp. 119
Author(s):  
Enike Dwi Kusumawati ◽  
Syam Rahadi ◽  
Sugeng Santoso ◽  
Dyah Lestari Yulianti
Keyword(s):  
Sperm Quality ◽  
Animal Husbandry ◽  
Randomized Design ◽  
Frozen Semen ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bull Sperm

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh lama thawing yang berbeda pada suhu 250C untuk mendapatkan kualitas spermatozoa semen beku sapi ongole yang optimal. Penelitian dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang. Metode Penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Setiap perlakuan thawing diberikan ulangan sebanyak 10 sampel semen beku sapi ongole. Hasil penelitian menunjukkan bahwa perlakuan dengan lama thawing 7, 15, 30 detik pada suhu 25oC memberikan perbedaan pengaruh yang sangat nyata (p<0.01) pada motilitas, viabilitas dan abnormalitas spermatozoa semen beku sapi ongole. Motilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 40,8%, viabilitas tertinggi diperoleh pada perlakuan P3 dengan rata-rata sebesar 82,39%, sedangkan abnormalitas terendah diperoleh pada perlakuan P3 dengan rata-rata sebesar 11,95%. Berdasarkan penelitian ini maka dapat disimpulkan bahwa thawing pada suhu 25oC dengan lama waktu 30 detik memberikan kualitas spermatozoa yang paling baik sehingga disarankan untuk melakukan thawing pada suhu 25oC dengan lama waktu 30 detik. Kata kunci: abnormalias, mortalitas, ongole, thawing, viabilitas ABSTRACT This study aims to determine the effect of different thawing time at 250C to get the sperm quality of frozen semen ongole bull. Research conducted at the Laboratory of Animal Husbandry Faculty Kanjuruhan University of Malang. The method of study by using Completely Randomized Design (CRD) factorial. Any treatment given repeated thawing of frozen samples of 10 times. The study show that treatment with time thawing 7 (P1), 15 (P2), 30 (P3) seconds at 25oC gives a very significant difference (P<0.01) on motility, viability and abnormalities of ongole Bull sperm. The highest motility and viability were obtained at P3 (40,8%) and (82,39%), while the lowest abnormalities obtained on P3 (11,95%). Based on this research it can be concluded that thawing at 25oC with 30 seconds to give the best quality sperm that is recommended for thawing. Keywords: abnormality, motility, ongole, thawing, viabilit

scholarly journals Factors Influencing the Popularity of Artificial Insemination of Mares in Europe

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 460 ◽  
Author(s):  
Alicja Kowalczyk ◽  
Ewa Czerniawska-Piątkowska ◽  
Marian Kuczaj
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
World Market ◽  
Factors Affecting ◽  
Current State ◽  
Horse Breeding ◽  
Frozen Semen ◽  
The Common ◽  
State Of Research

The purpose of this review was to analyze factors affecting the popularity of artificial insemination of mares in Europe in the context of sperm quality. Taking into account the prices of stallion semen on the world market, efficiency is important for the profitability of its use in artificial insemination programs in Europe. To increase the efficiency of a semen insemination facility, it is necessary to correctly and objectively assess the quality of semen. The available range of tools allows an effective evaluation of the potential fertility of a stallion. For several years, artificial insemination programs in Europe have been gaining popularity. However, the frequency of chilled or frozen semen use is still quite low. This is mainly due to the common, negative opinion about the effectiveness of the use of packaged insemination doses as opposed to natural insemination. Unfortunately, the quality of the semen offered often deviates from expectations, which results in unsatisfactory (and therefore unprofitable) pregnancy rates. This review presents the popularity structure of chilled and frozen semen use in European horse breeding as well as the current state of research on the effectiveness of semen production technology. It is shown that the popularity of using chilled semen in the artificial insemination of mares in Europe has been gradually increasing in the group of sport mares, while in the group of breeding mares, in recent years, frozen semen has been gaining popularity. In the remaining group of mares (not classified as sport or breeding), insemination with chilled semen has been dominant.

scholarly journals Evaluasi Status Reproduksi Sapi Hasil Persilangan Peranakan Ongole dengan Bali

2021 ◽  
Vol 21 (2) ◽  
pp. 207-214
Author(s):  
Jauhari Efendy ◽  
Peni Wahyu Prihandini ◽  
Tri Agus Sulistya ◽  
Almira Primasari
Keyword(s):  
Pregnancy Rate ◽  
Semen Quality ◽  
Reproductive Organs ◽  
Reproductive Organ ◽  
Reproductive Status ◽  
Bali Cattle ◽  
Cattle Reproduction ◽  
Rectal Palpation ◽  
Macroscopic Analysis

ABSTRACT. Tujuan penelitian untuk mengetahui status reproduksi hasil persilangan sapi peranakan ongole (PO) dan sapi bali (yang selanjutnya dinamakan sapi POBA) jantan dan betina melalui beberapa kegiatan lapang. Penelitian ini bersifat non eksperimental yang dilakukan secara eksploratif; menggunakan 20 ekor sapi berumur antara 32 sampai 36 bulan. Parameter yang diamati meliputi kualitas semen, kondisi organ reproduksi sapi betina dan angka kebuntingan. Hasil penelitian menunjukkan, kualitas semen sapi POBA rendah atau jelek berdasarkan hasil analisis secara mikroskopis maupun makroskopis; namun memiliki libido yang cukup tinggi yaitu rata-rata sekitar 4 menit 25 detik dari mulai mengendus atau didekatkan dengan ternak pemancing (teaser) sampai ejakulasi. Secara umum kondisi organ reproduksi sapi POBA betina normal, sehingga siap dijadikan indukan yang produktif. Sebanyak 66,67% menunjukkan perilaku estrus yang tampak terutama keluar lendir yang terlihat jelas menggantung di bibir vagina bahkan diantaranya mengejar dan/atau menaiki sapi-sapi lain. Namun hasil pemeriksaan kebuntingan (PKB) melalui palpasi rektal menunjukkan tidak ada sapi betina yang bunting. Dapat disimpulkan bahwa sapi POBA jantan infertil, yang diindikasikan melalui hasil pemeriksaan semen dan tidak adanya sapi betina yang berhasil bunting melalui perkawinan alam selama 3 (tiga) bulan. Sedangkan sapi betina POBA memiliki organ reproduksi normal serta menunjukkan ekspresi estrus yang baik.  (The evaluation of cattle reproduction status between crosses Ongole Crossbreed with Bali cows) ABSTRAK. The purpose of this research was to determine the reproductive status of the crossbred PO and Bali cattle (hereinafter referred to as POBA cattle) through several field activities. This research is non-experimental and carried out exploratory using 20 cattle aged 32 to 36 months. Parameters observed included semen quality, cows' reproductive organ condition, and pregnancy rate. The quality of POBA bulls semen was low or bad as indicated by the results of microscopic and macroscopic analysis; but had pretty high libido, which was an average of about 4 minutes and 25 seconds, starting to sniff or being brought near to teaser until ejaculation. In general, the reproductive organs of POBA cows were normal so that they were ready to become productive sires. As many as 66.67% showed estrus behavior, especially visible mucus that was hanging on the lips of the vagina, even among them chasing and/or riding other cattle. However, the results of pregnancy examination through rectal palpation showed that there were no pregnant cows. It can be concluded that POBA bulls are infertile, which was indicated by the results of semen examination and the absence of successfully conceived cows through natural mating for 3 (three) months. Meanwhile, POBA cows have normal reproductive organs and show good estrus expression.

scholarly journals Utilization of bull fertility-associated antigen to improve the quality of frozen bull semen

2020 ◽  
Vol 13 (10) ◽  
pp. 2112-2117
Author(s):  
Tri Wahyu Suprayogi ◽  
Hardijanto Hardijanto ◽  
Mas'ud Hariadi ◽  
Fedik Abdul Rantam ◽  
Win Darmanto
Keyword(s):  
Block Design ◽  
Field Testing ◽  
Seminal Vesicles ◽  
Second Phase ◽  
Genital Organ ◽  
Accessory Sex Glands ◽  
Bull Semen ◽  
Accessory Glands ◽  
Frozen Semen

Background and Aim: The implementation of artificial insemination (AI) is one of the strategies to use superior male semen optimally to improve the genetic quality of livestock. One of the factors that influence AI is a fertility-associated antigen (FAA). This research aimed to examine the effects of FAA extracted from the accessory sex glands of a bull from a slaughterhouse that was added in bull semen freezing medium to increase cattle (bull) fertilization. Materials and Methods: This research used a randomized complete block design. It consisted of two research phases, namely, explorative and experimental phases. The first phase involved determining the FAA molecular weight using the SDS-PAGE method, and the second phase consisted of laboratory and field testing, including testing the quality of frozen semen supplemented with FAA extracted from the accessory glands of a bull's genital organ from a slaughterhouse with various doses (0, 5, 10, and 15 μg in every 200 million progressively motile spermatozoa). Results: The results showed that the percentages of bull sperm motility between the groups without and with the additional administration of FAA with a dose of 5 μg did not significantly differ. However, there was a difference between the groups without and with the additional administration of FAA with doses of 10 and 15 μg. After further testing, the highest percentage of sperm progressive motility occurred at a dose of 15 μg/200 million progressively motile spermatozoa (P3), which was equal to 2.59±46.88b (%). Conclusion: This research found that not all of the accessory glands (seminal vesicles) of bulls taken from the slaughterhouse contain the FAA. An FAA level between the accessory glands (seminal vesicles) of one cattle to another is different. The addition of the FAA protein from the accessory sex glands of a bull's organ in cattle semen can improve fertility by increasing the percentage of viability, motility, intact plasma membrane of spermatozoa, and pregnancy rate of bulls and decreasing the sperm capacitation post-thawing.

scholarly journals Kualitas Sperma Beku Sapi Bali dalam Pengencer Air Kelapa Modifikasi dengan Berbagai Aras Dimethyl Sulfoxide (FROZEN SPERM QUALITY OF BALI BULLS IN MODIFIED COCONUT WATER EXTENDER WITH DIFFERENT DIMETHYL SULFOXIDE CONCENTRATION)

2019 ◽  
Vol 20 (1) ◽  
pp. 93
Author(s):  
Thomas Mata Hine ◽  
Kirenius Uly ◽  
Wilmientje Marlene Nalley ◽  
Heri Armadianto
Keyword(s):  
Dimethyl Sulfoxide ◽  
Liquid Nitrogen ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Optimal Concentration ◽  
Coconut Water ◽  
Low Molecular Weight ◽  
Frozen Semen ◽  
Artificial Vagina

Dimethyl Sulfoxide (DMSO) is one type of cryoprotectant which has a low molecular weight so that it is easier to enter cells when cryopreservation. The purpose of this study was to explore the optimal concentration of DMSO in modified coconut water (mCW) extender that were able to maintain frozen sperm quality of bali bulls. Semen was collected from two four-year old bali bulls by artificial vagina. Good quality semen diluted with mCW (young coconut water + 20% egg yolk + 7.5 % moringa leaf extract) and supplemented by 3, 5, or 7% DMSO. Semen was filled into 0.25 ml ministraw, and was incubated in a refrigerator at 5°C for four hours, frozen on the surface of liquid nitrogen for 10 minutes and then dipped into liquid nitrogen. The quality of post thawing sperm was measured 24 hours later by placing the ministraw of frozen semen into water at 37oC for 30 seconds. Data were analyzed by analysis of variance and continued with Duncan test. Postthawing observations showed that bali bulls sperm cryopreserved at 3% DMSO yielded higher motility and viability (p<0.05) i.e. 36 and 44.15%, than DMSO 5% i.e. 18 and 23.65%, and DMSO 7% i.e. 7 and 12.62%. The recovery rate of sperm cryopreserved at 3% DMSO was also higher (p<0.05) than DMSO 5 and 7%, successively 45.65, 23.06, and 8.86%. The results of this study concluded that the optimal concentration of DMSO in mCW diluent to maintain frozen sperm quality of bali bulls was 3%. 

scholarly journals THE EFFECT OF COGON GRASS ROOT ETHANOL EXTRACT ON REPRODUCTIVE ORGAN AND EPIDIDYMAL SPERM QUALITY OF MOUSE WITH DIFFERENT AGES

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Jaqueline Sudiman ◽  
Rini Widyastuti ◽  
Alkaustariyah Lubis ◽  
Tyagita Hartady ◽  
Mas Rizky Anggun Adipurna Syamsunarno ◽  
...  
Keyword(s):  
Treatment Group ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Ethanol Extract ◽  
Reproductive Organ ◽  
Semen Parameters ◽  
Epididymal Sperm ◽  
Treatment Groups ◽  
Grass Root

This study aims to determine changes related to epididymal sperm parameters in mice given cogon grass root ethanol extract (CGREE). The experiment was carried out by dividing three groups of mice aged 4-week old (early pubertal), 8-week old (adult), and 24-week old (old) as control and treatment groups. The treatment groups were given 115 mg/kg body weight of CGREE for 14 days. The mice were sacrificed on day 15 to asses reproductive organ indexes and quality of the epididymal sperm. Index of epididymis and ductus deferens indices significantly reduced in the 4-week old treatment group compared to control (0.23 vs 0.33; 0.08 vs 0.16,  respectively), and vesical seminal significantly reduced in 8- week old treatment group compared to control (0.76  vs  0.68). The epididymal sperm quality significantly decreased in all treatment groups, but the sperm concentration in early pubertal group showed an increase. These results indicate that CGREE can enhance several semen parameters in prepubertal and adult mice but this effect is not apparent in old mice.

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