scholarly journals Antituberculosis, antimicrobic and antioxidant properties of Persica vulgaris L. extracts

2020 ◽  
Vol 3 (2) ◽  
pp. e00125
Author(s):  
I. Mamatova ◽  
I. Askarov ◽  
M. Mamarakhmonov
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Mycobacterium Tuberculosis ◽  
Free Radicals ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Flower Extract

Peach is a medicinal plant which has many traditional applications uses against various diseases. In this study we have evaluated differences in tannins and flavonoids in the composition of flowers and peach leaves and their antioxidant properties. Antibacterial activity of the peach flower and leaf extract was investigated using Mycobacterium tuberculosis and E. coli by the disk diffusion method. Total fractions of flavonoids and tannins were obtained using ethanol and aqueous extraction, respectively. The antioxidant activity was evaluated using the adrenaline autooxidation test. The results have shown that the peach flower extract contains many flavonoids, tannins that probably account for better antimicrobial effects as compared with the peach leaf extract. This shows perspectives for the use of peach flowers for the treatment of many diseases, especially for tuberculosis, and other diseases associated with overproduction of free radicals.

scholarly journals ANTIBACTERIAL ACTIVITY OF TEA, BREADFRUIT, AND PIPER BETLE LEAVES EXTRACTS AGAINST Propionibacterium acnes AND ITS APPLICATION AS ANTI-ACNE MASK

2021 ◽  
Vol 18 (2) ◽  
pp. 105
Author(s):  
Angie Lucita ◽  
Tensiska Tensiska
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Phenolic Content ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Piper Betle ◽  
Bioactive Components ◽  
Disk Diffusion Method ◽  
Extract Concentration

Tea, breadfruit, and piper betle leaves contain bioactive components that have a function as an antibacterial. The purpose of this study was to determine the type of leaf extract that has the highest inhibition power against Proponibacterium acnes and determine the concentration of the selected extract for mask applications that have good physical characteristics and acceptable to panelists. Extraction of tea, breadfruit, and piper betel leaves were carried out with 96% ethanol solvent using maceration method for 24 hours and concentrated with a rotary evaporator. Thick extracts contribute to antibacterial activity by disk diffusion method and total phenolic content. The selected extract was formulated into a clay mask with concentration of 0.5%, 1%, and 1.5%. The result showed that breadfruit leaf extract had the highest inhibition power against Propionibacterium acnes which was 10.844 ± 0.327 mm (strong inhibition category) so that extract can be applied to clay mask. Breadfruit leaf extract concentration of 1.5% was the chosen formulation because it meets the standards, approved by the panelists and the most effective antibacterial for acne problems.

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (COFFEA CANEPHORA L.) LEAVES TO STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2019 ◽  
pp. 113-115
Author(s):  
ZAMHARIRA MUSLIM ◽  
YONANIKO DEPHINTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Effect ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Robusta Coffee

Objective: This research aims to analyze the ability of robusta coffee leaves fraction extract to inhibit the growth of Staphylococcus aureus and Escherichia coli and also determine the minimum inhibitory concentration (MIC). Methods: Antibacterial activity evaluated by the disc diffusion method observed in four types of fraction of extract robusta coffee leaves (n-hexane, ethyl acetate, ethanol, and water). Each extract divided into three various concentrations, 5%, 10%, and 15%. Determination of antimicrobial activity in vitro by the disk diffusion method. Results: Ethyl acetate fraction of coffee leaves extract produced the largest diameter zone of inhibition of bacterial growth compared to other extraction fractions of 17.28 mm in E. coli and 18.58 mm in S. aureus. The MIC of coffee leaves extract fraction water, ethyl acetate, and n-hexane on E. coli and S. aureus is 5%, while the fraction ethanol MIC is 10%. Conclusion: The antibacterial effect of ethyl acetate fraction of coffee leaves extract showed an antibacterial effect that was better than the fraction of n-hexane, ethanol, and water.

scholarly journals Phytochemical analysis and antibacterial activity of extract from lantana camara l. leaves

2021 ◽  
Author(s):  
Bondy Lourenço ◽  
Asimbawe Kiza ◽  
Abrão Amândio João ◽  
Clemência Félix Odala Niconte ◽  
Pompílio Armando Vintuar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lantana Camara ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Significant Difference ◽  
New Antibiotics

Abstract Background: Currently, several studies are being conducted to test antibacterial activity of various medicinal plants, in attempt to develop new antibiotics or to potentiate the action of antibiotics of known clinical use. In this research, phytochemical analysis and in vitro antibacterial activity of leaf extracts of Lantana camara L were performed.Methods The extracts were obtained through maceration with 90% ethanol. In phytochemical analysis, alkaloids, flavonoids, tannins and saponins were identified using specific reagents for each class.The antibacterial activity was analyzed using the disk diffusion method according to Kirby-Bauer, against Escherichia coli and Staphylococus aureus.Key findings The diameters of the inhibition halos varied between 0.7 and 12 mm, with an average of 8.26 mm for strains of S. aureus and there was no inhibition for E. coli. The results showed a significant difference between the inhibition halos and the tested concentrations for S. aureus. In conclusion L. camara leaf extracts are rich in bioactive compounds and have a good antibacterial potential against S. aureus and not for E. coli. And there was no relationship between the concentration of the extract and the diameter of the halo of inhibition of the evaluated microorganism.

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 59-68
Author(s):  
Sutomo Sutomo ◽  
◽  
Arnida Arnida ◽  
Fahriah Fahriah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 525
Author(s):  
Rafi’a Adinda Putri ◽  
Herny E.I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Paper Disk ◽  
Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has  benefits  for  many  diseases,  one  of  them  is  antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and  S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and  (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and  S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method   ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan  menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

scholarly journals Total polyphenol content and antioxidant capacity of allium plants and their correlation with antibacterial and antifungal activity

2021 ◽  
pp. 85-97
Author(s):  
S. S. Satorov ◽  
F. D. Mirzoeva
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Biologically Active ◽  
Diffusion Method ◽  
Active Components ◽  
Total Polyphenols ◽  
Disk Diffusion Method ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

Aim. To determine the total polyphenols and antioxidant capacity and their correlation with antimicrobial and fungicidal properties in some species of the genus Allium growing in Tajikistan.Materials and methods. The objects of the study were 15 kinds of the Amaryllidaceae family. Antibacterial onions were studied by the disk diffusion method against 4 types of microorganisms (test strains) and 1 type of yeast fungus. In the presence of total polyphenols, the content in μg of Gallic acid (GA) per ml of plant extract was measured. To determine the antioxidant activity, a solution of ABTS K2S2O8 was used. Trolox was used as a standard.Results. The maximum content of total polyphenols is characterized by ethanol extracts of A. Carolinianum, A. altaicum, A. elatum, A. oschaninii, A. ramosum, A. hymenorhisum. At the same time, their lowest amount was found in the bulbs of A. longicuspis. A. shoenoprasum, which is statistically significantly lower than in all the above-listed species of this plant group. The extracts from the stems of A. carolinianum, A. elatum, and A. pamiricum had the highest antioxidant effect. There is a direct weak correlation between biologically active components with antimicrobial and fungicidal activity in A. oshaninii and A. suworowii, depending on the altitude gradient.Conclusions. The results allow suggesting that the above-ground and underground parts of some wild-growing endemic species of the genus Allium have different degrees of antioxidant activity due to total polyphenols. The content of total polyphenols and the antioxidant capacity of extracts obtained from various plants in different in large ranges. In some cases, there are direct correlations between the content of polyphenols, antioxidant properties, antimicrobial and fungal properties.

scholarly journals Phytochemical Analysis and Antibacterial Activity of Extract From Lantana Camara L. Leaves

2021 ◽  
Author(s):  
Bondy Jorge Lourenço ◽  
Asimbawe Kiza ◽  
ABRÃO AMÂNDIO JOÃO ◽  
Clemência FÉLIX ODALA Niconte ◽  
POMPÍLIO ARMANDO Vintuar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lantana Camara ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Significant Difference ◽  
New Antibiotics

Abstract Background: Currently, several studies are being conducted to test antibacterial activity of various medicinal plants, in attempt to develop new antibiotics or to potentiate the action of antibiotics of known clinical use. In this research, phytochemical analysis and in vitro antibacterial activity of leaf extracts of Lantana camara L were performed.The extracts were obtained through maceration with 90% ethanol. In phytochemical analysis, alkaloids, flavonoids, tannins and saponins were identified using specific reagents for each class.The antibacterial activity was analyzed using the disk diffusion method according to Kirby-Bauer, against Escherichia coli and Staphylococus aureus. Results: The diameters of the inhibition halos varied between 0.7 and 12 mm, with an average of 8.26 mm for strains of S. aureus and there was no inhibition for E. coli. The results showed a significant difference between the inhibition halos and the tested concentrations for S. aureus. Conclusions: L. camara leaf extracts are rich in bioactive compounds and have a good antibacterial potential against S. aureus and not for E. coli. And there was no relationship between the concentration of the extract and the diameter of the halo of inhibition of the evaluated microorganism.

scholarly journals Antioxidant, Antimicrobial and Formulation of Borage (Borago officinalis) Seeds Oil and Leaves Extracts as Microemulsion

2021 ◽  
pp. 136-148
Author(s):  
Reem Yaghmour ◽  
Marwa Garajah ◽  
Ibrahim Kayali ◽  
Fuad Al-Rimawi
Keyword(s):  
Phase Diagram ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ternary Phase ◽  
Ternary Phase Diagram ◽  
Inhibition Zone ◽  
Borage Oil ◽  
Diffusion Method ◽  
E Coli ◽  
Antimicrobial And Antioxidant Activity

Aims: This study aims to investigate antioxidant and antibacterial activities of borage (seeds and leaves) extracts, and to prepare different topical microemulsion formulations using borage oil. Study Design: Borage seeds and leaves were collected from Hebron -Palestine, then the borage leaves and seeds were prepared for extraction and then extracted with Soxhlet using ethanol. The antimicrobial and antioxidant activity of the extracts were studied, and then Ternary Phase Diagram was constructed using the borage extracts (from seeds and leaves). Methodology: The seeds were cultivated upon their ripening season in April of 2016 from the Halhul mountains in Hebron/Palestine. Soxhlet method was used to extract borage seeds and leaves oil by using ethanol 95%. A ternary phase diagram was constructed by determining appropriate nonionic surfactant to assess the ability for microemulsion formulation and durability of each system. Tween 80 was found to be more suitable to solubilize each of borage seeds and leaves extracts compared with Tween 20 due to its prominent hydrophobic properties. The antibacterial activity was evaluated for both borage seeds and leaves extracts using a well diffusion method against Staphyloococcus aureus, Escherichia coli, &Candida albicans. Results: Results showed that the seeds extract has inhibition zone (12 mm) against S. aureus (gram positive bacteria) higher than inhibition zone that leaves extracts exhibited (7.5mm), but no significant effects observed for both extracts against E. coli and C. albicans. In addition, antibacterial activity for microemulsions formulation was measured against S. aureus, E. coli, and C. albicans. Results showed that there is minor activity against S. aureus when compared to PenicillinG and the pure seed oil or leaves extract. In contrast no activity was reported against E. coli and C. albicans. The antioxidant activity was further indicated by the quiet good ability to reduce the FRAP reagent for both extracts with the indication of higher seeds extract activity. This variation is explained by the higher seeds extract content of polyphenol, tocopherol and vitamin C than leaves extract. Conclusion: Borage seeds and leaves were extracted, and the extracts were showed antimicrobial and antioxidant activity and showed that they can be used in microemulsion using ternary phase diagram.

scholarly journals Study of in-vitro Antioxidant and Antibacterial Activity of Cow Urine from Different Altitudinal and Climatic Region of Nepal

2019 ◽  
pp. 1-8
Author(s):  
Dirgha Raj Joshi ◽  
Pramod Aryal ◽  
Mukesh Kumar Chaudhary ◽  
Prawin Kumar BK ◽  
Suvendra Prasad Yadav ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Free Radicals ◽  
Urine Samples ◽  
Diffusion Method ◽  
Tropical Region ◽  
Agar Disc ◽  
Cow Urine ◽  
Subalpine Region

Background: Cow urine has been widely used as therapeutic or additive agents in different parts of Nepal, mainly to treat infections, cuts, burns, etc. Cow urine has been considered as sacred and holy water and used in many rituals and worshipping purposes. Apart from therapeutic and rituals ceremony cow urines are also used in purification and disinfecting the houses. Objective: The aim of the study was to investigate the in-vitro antioxidant and antibacterial activity of cow urine from different altitudes across Nepal. Materials and Methods: Antioxidant activity of cow urines was carried out by using  2,2-Diphenyl-1-picrylhydrazyl (DPPH) as free radicals. The antibacterial activity of cow urines was tested by the agar disc diffusion method against Escherichia coli (gram-negative) and Staphylococcus aureus (gram-positive). Results: The urine sample from Muktinath (Subalpine region) showed the highest 44.8±0.21% inhibition against DPPH free radicals. The urine samples from Jajarkot and Marpha (Temperate zone) showed 39.70±0.43% and 37.30±0.15% inhibition against DPPH free radicals respectively. The urine samples from Palpa and Shyangja (Subtropical region) showed 30.00±0.24 and 34.4±0.01 percent inhibition of DPPH radicals. The samples from Butwal and Dhangadhi (Tropical region) showed the least percentage inhibition of 23.9±0.08 and 21.12±0.1 respectively. Amongst the entire sample, the subalpine region of cow urine (Muktinath 3710m) made known the highest inhibition zone such as 10.56±0.05mm against S. aureus and 7.46±0.15 against E.coli. Conclusion: Our study has concluded that cow urines have the antibacterial and antioxidant activities and vary in potency according to altitudinal and climatic differences. Hence cow urine from the subalpine zone has better antibacterial and antioxidant activity than that of lower altitudinal climatic zones.

scholarly journals Antibacterial and Antioxidant Activities of Polyeugenol with High Molecular Weight

2020 ◽  
Vol 20 (3) ◽  
pp. 722
Author(s):  
Erwin Abdul Rahim ◽  
Nur Istiqomah ◽  
Gilang Almilda ◽  
Ahmad Ridhay ◽  
Ni Ketut Sumarni ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Molecular Weight ◽  
Antioxidant Activities ◽  
Diffusion Method ◽  
E Coli ◽  
Ic50 Value ◽  
Inhibition Zones ◽  
Escherichia Coli Bacteria

This study was aimed to prepare polyeugenol with high molecular weight and to evaluate its antibacterial and antioxidant activities. First, polyeugenol was synthesized from eugenol in the presence of H2SO4-CH3COOH (4:1) as catalyst. The synthesized polyeugenol was weighed by using viscometer, revealing its high molecular weight of (7.76–21.9) × 105 g/mol. Furthermore, the antibacterial activity of the polyeugenol was conducted against Staphylococcus aureus and Escherichia coli bacteria. It was conducted by applying well diffusion method at 1, 2, 3, 4 and 5% concentrations to observe inhibition zones, in which the tests showed that the antibacterial activity of the polyeugenol against S. aureus were 17.42, 17.76, 18.79, 21.42 and 22.55 mm, while those against E. coli were 15.87, 17.23, 17.56, 18.24 and 19.21 mm, respectively. In short, these results indicated a strong antibacterial activity. Then, tests on antioxidant activity against free radical DPPH (2,2-diphenyl-1-pycrylhydrazyl) gave the IC50 value of 80.47 µg/mL, indicating a strong antioxidant activity. Therefore, the polymer synthesized in this work has a high potential to be applied in various biomedical applications.

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