scholarly journals Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

2017 ◽  
Vol 7 (1) ◽  
pp. 153
Author(s):  
Gaurav Mahesh Doshi ◽  
Manjushree Kundalik Pawar ◽  
Kajal Haribhai Chavda
Keyword(s):  
Gallic Acid ◽  
Cell Lines ◽  
Antibody Titre ◽  
High Performance ◽  
Assay Method ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid ◽  
Rutin And Quercetin

Background: The current research was undertaken on dried fruits of Capparis moonii to screen its potential for immunomodulatory and cancer indications with identification of phytoconstituents by chromatographic techniques.Methods: Methanolic (MECN), hydro-methanolic (HMECN) and aqueous extracts (AQCN) of Capparis moonii were subjected to high performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) after studying the total phenolic and flavonoid content by using rutin and gallic acid as standards respectively as well as undertaking powder characteristics and preliminary phytochemical screening. Immunomodulatory activities covered were hemagglutination antibody titre and delayed-type hypersensitivity reaction with the aid of sheep red blood cells (0.5×109) as antigens.  The extracts were studied for antioxidant potential. Anticancer prospects were focusing on in vitro cell lines screening (MCF 7 and HCT 15) by Sulforhodamine B assay method and potato disc assay.Results: The total phenolic and flavonoid content of MECM, HMECM and AQCM fruits extracts were found to be 0.20, 0.11 and 0.47 mg of gallic acid/g and 78.3, 18.8 and 64.4 mg of rutin/g respectively. Rutin and quercetin were confirmed by HPTLC and HPLC showing well resolved peaks. IC50 values in antioxidant studies were found to be significant with all the extracts. Significant immunomodulatory effect was noticed at 200mg/kg in both models (high antibody titre levels and decrease paw volume after 48 h). Unsatisfactory results were observed with selected cell lines and disc assay.Conclusions: Thus, selected fruits may probably have immunomodulatory potential due to presence of flavonols (rutin and quercetin).

scholarly journals Establishing Relationship between Vitamins, Total Phenolic and Total Flavonoid Content and Antioxidant Activities in Various Honey Types

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4399
Author(s):  
Norhasnida Zawawi ◽  
Pei Juin Chong ◽  
Nurul Nadhirah Mohd Tom ◽  
Nurkhairina Solehah Saiful Anuar ◽  
Salma Malihah Mohammad ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Extrinsic Factors ◽  
Biochemical Compounds ◽  
Phenolic And Flavonoid

Honey is a well-known natural sweetener and is rich in natural antioxidants that prevent the occurrence of oxidative stress, which is responsible for many human diseases. Some of the biochemical compounds in honey that contribute to this property are vitamins and phenolic compounds such as phenolic acids and flavonoids. However, the extent to which these molecules contribute towards the antioxidant capacity in vitro is inconsistently reported, especially with the different analytical methods used, as well as other extrinsic factors that influence these molecules’ availability. Therefore, by reviewing recently published works correlating the vitamin, total phenolic, and flavonoid content in honey with its antioxidant activities in vitro, this paper will establish a relationship between these parameters. Based on the literature, vitamins do not contribute to honey’s antioxidant capacity; however, the content of phenolic acids and flavonoids has an impact on honey’s antioxidant activity.

scholarly journals NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

2017 ◽  
Vol 9 (2) ◽  
pp. 166 ◽  
Author(s):  
Sayed A. El-toumy ◽  
Joslin Y. Salib ◽  
Nabila H. Shafik ◽  
Asmaa S. Abd Elkarim ◽  
Gihan A. Mick
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Secondary Metabolites ◽  
Gallic Acid ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Dpph Method ◽  
Phenolic And Flavonoid

<p><strong>Objective: </strong>The current study was to deal the isolation and identification of secondary metabolites from <em>Polygonum equisetiforme</em> and evaluation of antioxidant activity of its extract.</p><p><strong>Methods: </strong>The methanol-water extract (7:3) of the air-dried aerial parts of <em>Polygonum equisetiforme</em> was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for <em>in vitro</em> antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.</p><p><strong>Results: </strong>Ten secondary metabolites were isolated from <em>Polygonum equisetiforme</em> in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-<em>O</em>-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-<em>O-β</em>-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-<em>O-β</em>-D-glucucorinde methyl ester (4), quercetin 3-<em>O-</em>β-D-glucopyranoside (5), quercetin 7-<em>O-β-</em>D-glucopyranoside (6),<em> </em>quercetin(7)<sub>, </sub>myricetin (8), <em>P</em><sub>-</sub>methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of <em>P. equisetiforme</em> extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC<sub>50 </sub>= 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.</p><p><strong>Conclusion: </strong><em>Polygonum equisetiforme</em> is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.</p>

scholarly journals Effect of Plant Extracts on the Characteristics of Silver Nanoparticles for Topical Application

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1244
Author(s):  
Ioanna K. Siakavella ◽  
Fotini Lamari ◽  
Dimitrios Papoulis ◽  
Malvina Orkoula ◽  
Patroula Gkolfi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Colloidal Stability ◽  
Skin Barrier ◽  
Physicochemical Characteristics ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Laser Scattering ◽  
The Impact ◽  
Phenolic And Flavonoid

Silver nanoparticles (AgNPs) were synthesized using hydroalcoholic extracts of dittany (Origanum dictamnus), sage (Salvia officinalis), sea buckthorn (Elaeagnus rhamnoides, syn. Hippophae rhamnoides), and calendula (Calendula officinalis) as reducing agents. AgNPs synthesized using NaBH4 and citric acid were used as control. The impact of the origin of the extract and preparation conditions (light, temperature, reaction time) on the properties of the synthesized AgNPs was investigated. The structure, morphology, composition, physicochemical characteristics, and colloidal stability were characterized using dynamic laser scattering (DLS), ultraviolet-visible spectrophotometry (UV–/Vis), XRD, X-ray fluorescence (XRF), TEM, and FTΙR. The reduction of total phenolic and flavonoid content of the extracts after the reaction of AgNPs synthesis was also determined. Low IC50 values for all types of AgNPs revealed good antioxidant activity, attributable to the phenolic and flavonoid content of their surface. The results suggest that plant extract selection is important to the green synthesis of AgNPs because it affects the kinetics of their synthesis as well as their morphology, physicochemical characteristics, and colloidal stability. In vitro permeation studies on porcine skin revealed that AgNPs remained at the upper layers of stratum corneum and did not penetrate the skin barrier after 4 h of cutaneous application suggesting the safety of their application on intact skin for a relatively short time.

Extraction of Phenolic Compounds of six Algerian date (Phoenix dactylifera L.) cultivars from Ain-Saleh region, using Reflux method and Screening of Antioxidant Activity in vitro

2021 ◽  
pp. 161-167
Author(s):  
Habchi A. ◽  
Dekmouche M. ◽  
Hamia C. ◽  
Saidi M. ◽  
Yousfi M. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Methanol Extract ◽  
Phoenix Dactylifera ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phoenix Dactylifera L ◽  
Phenolic And Flavonoid

This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).

Comparative Antioxidant Potential of Bark and Leaves of Terminalia arjuna (Roxb) Wight & Arn from Himachal Pradesh

2017 ◽  
Vol 6 (1) ◽  
pp. 27 ◽  
Author(s):  
Vikas Kumar ◽  
Kamal Dev ◽  
Anuradha Sourirajan ◽  
Prem Kumar Khosla
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Terminalia Arjuna ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

The present study aimed to compare antioxidant activities, total phenolic and flavonoid content present in leaves and bark of Terminalia arjuna and check whether there is any correlation between phenolic content and flavonoid content with antioxidant activities or not. Phytochemical screening of ethanolic extract of leaves and bark revealed the presence of phenols, flavonoids, tannins, carbohydrates, glycosides, saponin, phytosterols and phytosteroids. Total phenolic content was found to be higher in bark (272.71±3.18 mg/g gallic acid equivalents) as compared to that of leaves (95±3.11 mg/g gallic acid equivalents). Similarly, flavonoid content of ethanolic extract of bark was found to be higher (203.95±5.13 mg/g rutin equivalents) than that of leaves (87.625±4.28 mg/g rutin equivalents). DPPH activity of ethanolic extract of bark (IC50-17.41 µg/ml) was more than that of leaves (IC50-20.22µg/ml). FRAP activity of bark (IC50- 4.781 µM Fe (II) equivalents) is more than that of leaves (IC50-7.572 µM Fe (II) equivalents). Nitric oxide (NO) scavenging activity of bark (IC50-12.87 µg/ml) was higher than that of leaves (IC50-13.91 µg/ml).The present study clearly showed that there is a correlation between total phenolics, flavonoid contents and antioxidant activity of leaves and bark of T. arjuna. The phenolic compounds and flavonoids could be major contributor to antioxidant activity of T. arjuna. Keywords: Terminalia arjuna, DPPH, FRAP, NO, IC50, Antioxidants

scholarly journals PHYTOTOXIC, ANTIOXIDANT, ANTIBACTERIAL, AND ANTILEISHMANIAL ACTIVITIES OF EUPHORBIA HIRTA FROM CHITWAN DISTRICT NEPAL

2019 ◽  
pp. 146-149
Author(s):  
KHAGA RAJ SHARMA
Keyword(s):  
Plant Extract ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Euphorbia Hirta ◽  
Phytotoxic Activity ◽  
Phenolic And Flavonoid

Objectives: The aim of this study is to investigate the antimicrobial, antioxidant, antileishmanial, and phytotoxic activities and estimation of total phenolic and flavonoid contents in methanol extract of Euphorbia hirta collected from Chitwan district of Nepal using in vitro studies. Methods: Methanol extract of the plant was prepared by cold percolation method. Screening of secondary metabolites was performed by color differentiation methods. The in vitro antioxidant potential of the plant extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl assay in which percentage scavenging and inhibitory concentration (IC50) were calculated. Furthermore, antileishmanial and phytotoxic activity was evaluated by adopting the standard protocols. Phenolic and flavonoid content was estimated using Folin–Ciocalteu phenol reagent and aluminum chloride colorimetric method, respectively. The antimicrobial activity was evaluated by agar well diffusion method. Results: The antileishmanial activity of the methanolic extract of E. hirta showed low leishmanicidal activity IC50 68.1±1.2 μg/ml standard deviation (SD) as compared to the standard drugs amphotericin B 0.29±0.05 μg/ml SD and pentamidine 5.09±0.09 μg/ml SD. The radical scavenging activity shows IC50=29.23±0.21 μg/ml SD which is found potent than the standard ascorbic acid IC50=45.93 μg/ml. The extract of E. hirta contains high value of total phenolic content milligram gallic acid equivalent per gram (138.10±4.90 mg GAE/g SD) and flavonoid content milligram quercetin equivalent per gram (11.54±0.00 mg QE/g SD). The plant extract exhibits weak antibacterial activity against Staphylococcus aureus (9.0 mm), Bacillus subtilis (11.0 mm), Escherichia coli (9.0 mm), and Salmonella Typhi (9.0 mm) at a concentration of 10.0 mg/ml in which 50 μl was loaded per well. Plant extract exhibits moderate phytotoxic activity with the number of fronds 3, 10, and 14 against the different concentrations of plant extract. Conclusions: The preliminary results of this study have put forward E. hirta into promising herbs with good antioxidant activities, moderate phytotoxic activity, and potential source of antibacterial agent with a significant amount of total phenolic and flavonoid contents. Although the plant extract shows the potent biological activity, further studies are needed to assess its mechanism of action.

scholarly journals High Performance Liquid Chromatography Analysis Using Rutin Marker and Estimation of Phenolic & Flavonoid Compounds in the Extracts of Indian Medicinal Plant Morus nigra L.

2019 ◽  
Vol 31 (8) ◽  
pp. 1801-1804
Author(s):  
Sunita Singh ◽  
Alka Tripathi ◽  
V.K. Lal ◽  
Dhananjay Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gallic Acid ◽  
High Performance ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Chromatography Analysis ◽  
Morus Nigra ◽  
Standard Solution ◽  
Plant Leaf Extract

Morus nigra L. (black mulberry) belongs to moraceae family of Indian medicinal plants. Black mulberry has significant anticancerous, antioxidant, antidiabetic, antimicrobial and antiobesity activities. Rutin marker was used for high performance liquid chromatography technique. We used gallic acid and quercetin for phenolic compound determination and flavonoid content determination in plant leaf extract. Plant extract of this species was analyzed quantitatively and qualitatively through high pressure liquid chromatography. Rutin compound was the main marker, which is a flavonoidal compound. Standard solution (1 mg/mL) of rutin was prepared by using CH3OH. Methanol:water (80:20, v/v) ratio was used to dissolve powdered M. nigra plant leaves (100 mg), in which 0.01 mg/g rutin content was found. Total phenolic content in M. nigra was found 43.15 ± 0.68 mg/g GAE (gallic acid equivalents). Total flavonoid content in M. nigra was found 5.8 ± 0.46 mg/g QE. It was found that the M. nigra has not significant rutin potency and found to be as 0.01 mg/g.

scholarly journals PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF WHOLE PLANT EXTRACTS OF SESUVIUM PORTULACASTRUM L

2018 ◽  
Vol 11 (1) ◽  
pp. 322
Author(s):  
Sathvika Chintalapani ◽  
Swathi M. S. ◽  
Mangamoori Lakshmi Narasu
Keyword(s):  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ether Extract ◽  
Sesuvium Portulacastrum ◽  
Flavonoid Content ◽  
Diethyl Ether Extract ◽  
Phenolic And Flavonoid

  Objectives: The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds, and determine the antioxidant capacity of the Sesuvium portulacastrum L. (S. portulacastrum).Methods: The crude bioactives were extracted from the dried powder of S. portulacastrum in an orbital shaker using ethanol, methanol, acetone, hexane, and diethyl ether solvents. Rotaevaporator was used to concentrate the extracts. Total phenolic and flavonoid content was estimated spectrophotometrically using Folin-Ciocalteu and aluminum chloride reagents, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were used to determine the in vitro antioxidant capacity.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged from 14.42 (ethanol extract) to 54.05 (diethyl ether extract) mg GAE/g dry weight. Total flavonoid content expressed as quercetin equivalents (QE) ranged from 22.03 (hexane extract) to 56.70 (methanol extract) mg QE/g dry weight. Antioxidant activity determined by different assays is highest in diethyl ether extract. A positive correlation (0.7241≤ r ≥0.8419) was found between the TPC and antioxidant activity determined by DPPH, ABTS, and H2O2 scavenging assays. Furthermore, there is a strong correlation (0.722≤ r ≥0.999) between all the pairs of antioxidant assays.Conclusion: Diethyl ether extract showed the highest TPC and antioxidant potential among all the extracts of S. portulacastrum. Further research has to be done to isolate the pure bioactive compound that has high antioxidant potential.

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