scholarly journals An experimental study to test the haemostatic effect of Aloe vera

2019 ◽  
Vol 8 (4) ◽  
pp. 717
Author(s):  
Ramesh H. ◽  
Jyothi C. H.
Keyword(s):  
Wound Healing ◽  
Coming Out ◽  
Leaf Extract ◽  
Aloe Vera ◽  
Test Group ◽  
Water Bath ◽  
Control Group ◽  
Traditional System ◽  
Perennial Plant

Background: Blood clotting is a process which prevents blood loss during injuries. Blood clots even when it is coming out from the blood vessels. Aloe vera is a perennial plant found all over India. It is commonly used in traditional system of medicine for treatment of wound healing, mouth ulcers, constipation, skin wrinkles and diabetes mellitus It is also used as antioxidant and antimicrobial agent. As it is used in traditional system of medicine for wound healing, this study was under taken to test the possible haemostatic effect of aloe vera.Methods: 12 rats were divided into two groups (control and test) with 6 rats in each group. Determination of Bleeding Time (BT), rat tail was warmed for one minute in water at 40˚C and then dried. A small cut was made in the middle of the tail with a scalpel. In test group, a drop of aloe vera leaf extract was applied on the injured area immediately after making the cut in the middle of the tail, where as in the control group nothing was applied, and BT estimated. Determination of Clotting Time (CT), 12 test tubes were arranged in water bath at 37˚C. Control Group: 0.4 ml of blood was collected from each rat in the control group and added to 6 test tubes kept in the water bath. Test group: For the remaining 6 test tubes 0.1 ml of aloe vera leaf extract was added. 0.4 ml of blood collected from the test group was added to these test tubes. The CT was estimated for both control group and test group.Results: The results were statistically analyzed by using unpaired t-test. The reduction in BT and CT for test group was statistically highly significant (p<0.001) compared to control group.Conclusions: In this study aloe vera leaf extract significantly reduced both BT and CT in the test group.

scholarly journals Evaluation of antiulcerogenic activity of combination of Aloe vera and Aegle marmelos in indomethacin induced gastric ulcers

2019 ◽  
Vol 8 (3) ◽  
pp. 453
Author(s):  
Leela Hugar ◽  
Ramesh H.
Keyword(s):  
Side Effects ◽  
Leaf Extract ◽  
Aloe Vera ◽  
Test Group ◽  
Peptic Ulceration ◽  
Gastric Ulcers ◽  
Control Group ◽  
Albino Rats ◽  
Aegle Marmelos ◽  
Ulcer Index

Background: Most important adverse effect of NSAID is peptic ulceration. Even though H2 blockers and proton pump inhibitors are effective in preventing NSAID associated peptic ulceration, they are not without side effects. Hence there is a need for drugs which are effective in preventing NSAID induced peptic ulcer without producing side effects. Two plant products Aloe vera leaf extract and Aegle marmalos leaves are commonly used in Indian traditional medicine for treatment of peptic ulcers. Hence this study is undertaken to assess the antiulcerogenic potential of combination of these two drugs in comparison with ranitidine in preventing NSAID induced peptic ulcers.Methods: 18 albino rats were divided into 3 groups of 6 rats each. Group A: received ulcerogen only. Group B: pretreated with ranitidine before exposing to ulcerogen. Group C: pretreated with combination of Aloe vera and Aegle marmelos before exposing to ulcerogen. Two doses of indomethacin were administered at an interval of 15 hrs. Animals were sacrificed 6 hrs after the second dose of Indomethacin. Number of ulcers was noted, and ulcer index was calculated.Results: There was significant reduction in total score, mean score and ulcer index in ranitidine pretreated group and test compound group as compared to control group. Even though the total score and ulcer index in test group were lesser as compared to standard control group, it was not statistically significant.Conclusions: Combination of Aloe vera leaf extract and Aegle marmelos leaf extract produced very significant protection against indomethacin induced gastric ulcer.

scholarly journals Evaluation The Anti-Inflammatory Effect of Prepared Aloe Vera Gel in Sulcular Full Thickness Flap Induced in Rats Model

2021 ◽  
Vol 21 (1) ◽  
pp. 75-85
Author(s):  
Amal Hanna Aziz ◽  
Keyword(s):  
Wound Healing ◽  
Control Level ◽  
Aloe Vera ◽  
Test Group ◽  
Control Group ◽  
Full Thickness ◽  
Post Surgery ◽  
Aloe Vera Gel ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Background: Aloe Vera contains many inorganic electrolytes like iron, potassium, chromium, copper, sodium, calcium, and zinc that are vital parts that induced the wound healing process as a result of the ability to prevent injury to the epithelium and promote healing of injured tissues. It improves healing by increasing the blood supply and oxygen to the area of injury tissue. Objective: To evaluate the anti-inflammatory effect of Aloe Vera on wound healing in rats with full thickness flap and insertion of Aloe vera gel at the site of injury throughout analysis of the activity level of serum interleukin-1beta (IL-1β). Patients and Methods: Fifty five male rats were randomly divided into three groups: A-Aloe vera gel (25 rats), subdivided into (5) subgroups as a test group, B- Chlorhexidine gel (25 rats) was also subdivided randomly into (5) subgroups as a test group. Each subgroup (5rats) was exposed to surgery and filled with (AV) and CHX gel in both. C-non-surgical control group (5 rats) to obtain baseline data. A sulcular full thickness flap incision was carried out between the two maxillary central incisors. Blood samples for immunological studies were collected by cardiac puncture, at times (3, 5, 7, 10, and14 days) for estimation of serum (IL-1β) levels using Indirect ELISA Technique in all groups. Results: A highly significant decrease of serum IL-1β in rat group (A) within 3 and 5days post-surgery, then the concentration of this cytokine started to increase on 7,10and 14days post-surgery, which is statistically non significantly affect with the control group (C), where it reaches the level of the control level before surgery. Conclusion: Aloe vera shows anti-inflammatory effects by decreasing the inflammation of periodontal tissue post-surgery which could accelerate healing based on a significant increase in the level of serum (IL-1β), reaching the control level before surgery at 14days, post-surgery that indicated positive results of this study. Keywords: Aloe Vera, chlorhexidine, pro-inflammatory (IL-1β), surgical full thickness flap

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Cografts of artificial dermis matrix and autogenetic split-thickness of repaired skin in severe hand wounds in patients with deep burns

2014 ◽  
Vol 66 (1) ◽  
pp. 173-178
Author(s):  
Hongqi Liu ◽  
Yan Li ◽  
Deqian Sha
Keyword(s):  
Wound Healing ◽  
Hand Function ◽  
Donor Site ◽  
Test Group ◽  
Healing Time ◽  
Whole Body ◽  
Control Group ◽  
Large Area ◽  
Artificial Dermis ◽  
Thickness Skin

The aim of this paper was to evaluate the effect of using artificial dermis matrix plus autologous split-thickness skin (ADM and ASTS) in the treatment of deep-burns in hands of severely burned patients?We recruited a total of 58 patients with large area burns greater than 80% that were eschar-excised. Twenty-eight of them were treated with ADM and ASTS (test group); 30 were treated with autologous medium-thickness skin (AMTS) (control group). The healing time of the hand wound was noted, clinical and photographic evaluations were performed, and a Jebsen-Taylor hand function test was compared and analyzed in the two groups. The wound healing time in the test group (24.22?3.34 days) were longer than that of the control group (13.42?3.36 days) and statistically significant. The healing time of skin graft donor sites was shorter than that of the control group (7.14?1.63 vs. 14.28?2.37 days) and statistically significant (P<0.05). The 3rd and 6th month follow-up with clinical and functional evaluations revealed no differences between the two groups. In addition, there was no obvious scar formation and less pigmentation in either group. The repair of deeply burned hands with artificial dermis matrix was beneficial to both wound healing and the donor site, and was beneficial to the whole body rehabilitation of severely burned patients.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals The Effect of Aloe Vera Extract to Extensive Lesion and Expression of Transforming Growth Factor (TGF-β) on Alkaline Chemical Trauma Cornea Model

2018 ◽  
Vol 1 (2) ◽  
pp. 1-17
Author(s):  
Fahcreza ◽  
Elsa Iskandar ◽  
Rachmat Hidayat ◽  
Petty Purwanita ◽  
Anang Tribowo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wistar Rats ◽  
Aloe Vera ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Significant Difference ◽  
The Difference ◽  
In Cells

Abstract Background: Chemical trauma to the cornea is an emergency condition of the eye that requires early diagnosis and good treatment. Alkaline have ability to saponify fatty acids in cells and cell membranes which can make penetration into the stroma and destroy proteoglycans and collagen in cells. Aloe vera (AV) contains several active substances that are reported to have anti-inflammatory, immunomodulatory, and wound healing effects. AV has been reported to accelerate the healing process of corneal epithelial defects by increasing fibroblast proliferation, collagen production and growth factor production. This study aims to determine the difference between the effect of aloe vera extract with a concentration of 10%, 20%, 40% and BSS on the healing of extensive corneal lesions in white wistar rats alkaline trauma models. Method: This study was an experimental study with a pre and posttest only with control group design in vivo approach to 30 Wistar white rats which were divided into 5 treatment groups for 3 days. Comparative analysis of effectiveness using the ANNOVA test or the Kruskal Wallis test and continued by the post hoc test. Results: Based on the one way ANOVA test there was a statistically significant difference in effectiveness between the five treatment groups on the percentage of corneal wound healing area and TGF-β expression with an assessment of p = 0,000 each. The administration of alloevera (AV) concentration of 20% had a significant difference in percentage of healing of corneal lesions and TGF-β expression compared with other treatment groups with p = 0,000 each. Large differences in the area of corneal lesions in the 40% AV group were -0.45 in the BBS group, 0.146 in the 10% AV group, 0.493 in the 20% AV group. The difference in the AV group 10% was 0.30 in the BBS group, -064 in the AV group 20%, and -0.14 in the AV group 40%. However, TGFβ expression in the normal control group that did not receive treatment was 54.94 (53.21-56-12). TGFβ levels in the BSS group were 10.44, the 10% aloe vera group was 25.43, 47.99 for the 20% aloe vera group and 37.95 for the 40% aloe vera group. Conclusion: There is a difference between the effect of aloe vera extract with concentrations of 10%, 20%, 40% and BSS on the extensive healing of corneal lesions in white wistar rats with alkaline chemical trauma models.

scholarly journals EFFECT OF ALOE BARBADENSIS MILLER WHOLE LEAF EXTRACT ON HYPERGLYCEMIA AND INSULIN RESISTANCE IN LOW DOSE STREPTOZOTOCIN INDUCED TYPE 2 DIABETIC RATS

1969 ◽  
Vol 3 (2) ◽  
pp. 350-355
Author(s):  
MEENA GUL ◽  
MUHAMMAD MAZHAR HUSSAIN ◽  
AYESHA BABER ◽  
AMJAD ZAMAN ◽  
MUSRAT ZAHRA
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Plasma Glucose ◽  
Leaf Extract ◽  
Low Dose ◽  
Aloe Vera ◽  
Control Group ◽  
Sprague Dawley ◽  
Type 2 Diabetic

BACKGROUND: Managing diabetes is difficult due to the number of side effects associated with drugsused for its treatment. There it is a need of an hour to look for indigenous plants which are safe and costeffective. Present study was planned to determine the effect of Aloe vera whole leaf extract and/orRosiglitazone on plasma glucose, insulin and insulin resistance in type 2 diabetic Sprague-Dawley rats.DESIGN: Randomized control trailPLACE AND DURATION OF STUDY: This study was conducted from April 2009 to Oct 2010 at theDepartment of Physiology Army Medical College, Rawalpindi in collaboration with National Institute ofHealth (NIH) Islamabad.MATERIAL AND METHOD: Type 2 DM was induced in 60 healthy Sprague-Dawley rats by feedinghigh fat diet for 2 weeks and injecting a low dose (35mg/kg) of streptozotocin intra peritoneally. Type 2diabetic rats were randomly divided into four groups, each group having 15 rats and were labeled as diabeticgroup, Aloe vera group, rosiglitazone group and combined group. The diabetic group was injected normalsaline, Aloe vera group was treated with Aloe vera whole leaf extract in dose of 300mg/kg body weight,rosiglitazone group was given 5mg/kg body weight of rosiglitazone I/P and combined group diabetic ratswere treated with 150mg/kg body weight of Aloevera extract and 2.5mg/kg body weight of rosiglitazone(halfof their effective dose) for 21 days.RESULTS: A significant reduction (p<0.001) in plasma glucose (73%), insulin (32%) and TG/HDL ratio(81%) was analyzed in combined groupascompared to diabetic control group. \CONCLUSION: The maximum impact in lowering plasma glucose, insulin and TG/HDL ratio wasrecorded in combined group, followed by rosiglitazone group and then Aloevera group.KEYWORDS:T2DM. Aloe vera, insulin resistance

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

EFFECTIVENESS TEST OF ANTIBACTERIAL EXTRACT MINT LEAF (Mentha piperita L.) INHIBIT GROWTH IN Streptococcus sanguinis

2021 ◽  
Vol 1 (Volume 1 No 1) ◽  
pp. 49-59
Author(s):  
Herryawan ◽  
Rahmadaniah Khaerunnisa ◽  
Fadhilah Nuraini Fajri
Keyword(s):  
Leaf Extract ◽  
Large Diameter ◽  
Test Group ◽  
Mentha Piperita ◽  
Diffusion Method ◽  
Control Group ◽  
Oral Diseases ◽  
Streptococcus Sanguinis ◽  
Study Results ◽  
Mint Leaf

Streptococcus sanguinis (s.sanguinis) is one of the normal flora in the oral cavity. These bacteria act as a pioneer in forming plaques that cause most dental and oral diseases. It takes an antibacterial agent to be able to inhibit the clips of the plaque. One of the herbal plants that have been known to have antibacterial power is the mint leaf (Mentha Piperita L.). The antibacterial potency of this plant comes from its chemical compound content, such as flavonoids, polyphenols, tannins, and menthols. This study aimed to determine the inhibitory of mint leaf extract on the growth of Sanguinis. An experimental laboratory carries out research. Tests were carried out using the diffusion method in four test groups, namely mint leaf extract with a concentration of 2.5%, 5%, 7.5%, and 10%, and one control group that uses DMSO. Each treatment group repeated five times on the Mueller-Hinton media (MHA) using disk paper and incubated for 24 hours. The inhibition zone can be seen in the transparent area formed around the disk paperand then measured using the Sorong term to find a large diameter. Statistical analysis was carried out using the Kruskal-Wallis test. The study results that the largest inhibitory zone diameter was obtained in a test group with a concentration of 10% (16.39 mm), while the lowest diameter in the control group (4.89 mm) 10% concentration was the highest among all test groups. The DMSO did not have an antibacterial effect. The higher the concentration of an antibacterial compound, it will also increase its effectiveness.

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