scholarly journals Determination of antioxidant activity of beetroot lip balm by Fluorimetry and HPTLC methods

2021 ◽  
Vol 6 (4) ◽  
pp. 197-200
Author(s):  
N Sunitha ◽  
S Manohar Babu ◽  
B Appa Rao
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Emission Spectra ◽  
Anti Oxidant ◽  
Acid Sample ◽  
Hptlc Method

Lip balm was prepared and its antioxidant activity was determined by PFRAP, CUPRAC, Flourimetry methods and results of emission spectra was examined and fournd to possess almost same concentration of 0.3 ug/ml. And the ascorbic acid was determined by HPTLC method and the Rvalue of the lip balm was found to be 0.98 and is near to the abscorbic acid sample. And hence proved that beetroot lip balm was found to possess antioxidant activityThe anti oxidant activity was determined and was found to be simple, novel and economical.

scholarly journals HPTLC method for simultaneous determination of ascorbic acid and gallic acid biomarker from freeze dry pomegranate juice and herbal formulation

2019 ◽  
Vol 27 (7) ◽  
pp. 975-980 ◽  
Author(s):  
Prawez Alam ◽  
Y.T. Kamal ◽  
Saleh Ibrahim Alqasoumi ◽  
Ahmed Ibrahim Foudah ◽  
Mohammed Hamed Alqarni ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gallic Acid ◽  
Simultaneous Determination ◽  
Pomegranate Juice ◽  
Herbal Formulation ◽  
Hptlc Method

Simultaneous analytical efficiency evaluation using HPTLC method for analysis of syringicacid and vanillic acid and their anti-oxidant capacity from methanol extract of Ricinus communis Linn. and Euphorbia hirta Linn

2020 ◽  
Author(s):  
Pradeep Singh ◽  
Muhammad Arif ◽  
Abdul Qadir ◽  
Pushpendra Kannojia
Keyword(s):  
Antioxidant Activity ◽  
Vanillic Acid ◽  
Methanol Extract ◽  
Ricinus Communis ◽  
Radical Scavenging ◽  
Syringic Acid ◽  
Euphorbia Hirta ◽  
Anti Oxidant ◽  
Marker Compounds ◽  
Hptlc Method

Abstract Background Well-known medicinal plants Ricinus communis Linn. and Euphorbia hirta Linn. have been traditionally used to treat a variety of ailments in the India and other countries. Objective In present research, a simple, rapid, reliable and accessible HPTLC technique has been established for concurrent quantification of phenolic compounds (syringic acid and vanillic acid)and evaluation of their anti-oxidant capacity. Method The chromatographic separation was conceded on pre-coated silica gel plates F254 as the stationary phase. The appropriate mobilephasecombination toluene: ethyl acetate: formic acid (7:2.5:0.5 v/v/v) were developed to expand the plates which separated components according to the marker compounds. Further, the anti-oxidant potential of methanol extract R. communis (MERC) and E. hirta (MERH) were also assessed with (DPPH) by using UV spectrophotometer. Results Densitometric scanning was performed by Camag scanner V and measured at two different wave length 272 nm and 318 nm. The marker compounds were practically resolved with RF 0.5 ± 0.04 for syringic acid and 0.6 ± 0.06 for vanillic acid. The results obtained in the study of anti-oxidant activity of methanol extract R. communis(MERC) and E. hirta (MERH) were showed significant free radical scavenging capacity against DPPH generated free radicals. Conclusions The developed HPTLC method was validated for accuracy, linearity, precision, and specificity. Both the extract has revealed considerable antioxidant activity. The reported existing phenolic and flavonoids compounds are responsible for antioxidant activity of plant extract.

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals Nutritional and sensory evaluation of wheat-maize cookies enriched with African walnut (Tetracarpidium conophorum) seed protein isolate

2020 ◽  
Vol 15 (1-2) ◽  
pp. 54-64
Author(s):  
Jonathan Oluwayemisi Foluke Awofadeju ◽  
Abiodun Adekunle Olapade
Keyword(s):  
Ascorbic Acid ◽  
Seed Protein ◽  
Protein Isolate ◽  
Sensory Attributes ◽  
Nutritional Composition ◽  
Overall Acceptability ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Acid Sample

The study examined the nutritional composition and sensory attributes of cookie. In this study, blends of commercial wheat (CWF), yellow maize flours (YMF) and African walnut protein isolate (AWPI) were optimized for cookie production. The cookies were studied in a completely randomized design and assessment of the proximate composition was conducted. The best cookie samples with high protein and fiber, low fat and highest rating in sensory attributes were selected for the determination of vitamins and mineral composition. The result revealed that the control cookie was highest in moisture, fat and carbohydrate but lower in protein, ash and fibre. Enriched cookie 36.69g CWF, 39.57g YMF, 23.74g AWPI and 42.86g CWF, 42.86g YMF, 14.29g AWPI were highest in protein and fibre, respectively while 55.56g CWF, 33.33g YMF, 11.11g AWPI was low in fat. Overall acceptability was highest in control cookie followed by enriched cookie 55.56g CWF, 33.33g YMF, 11.11g AWPI. The vitamin composition of the selected enriched samples show that sample 42.86g CWF, 42.86g YMF, 14.29g AWPI had the highest value of 2.53 mg/100 g in ascorbic acid; sample 55.56g CWF, 33.33g YMF, 11.11g AWPI scored the highest value of 0.14, 0.07 and 1.33 mg /100 g in thiamine, riboflavin and niacin respectively, while sample 36.69g CWF, 39.57g YMF, 23.74g AWPI scored highest in vitamin A (276.67 mg/100g). The cookie sample 36.69g CWF, 39.57g YMF, 23.74g AWPI shows the highest values of 130.00, 0.043, 9.77, 333.33 and 85.00 for calcium, manganese, iron, sodium and phosphorus, respectively. The study recommends the use of flour proportion in sample 55.56g CWF, 33.33g YMF, 11.11g AWPI as an improvement of the nutritional and sensory attributes of enriched cookie.

scholarly journals DEVELOPMENT OF POLYHERBAL WITH ANTIOXIDANT ACTIVITY

2018 ◽  
Vol 11 (8) ◽  
pp. 483
Author(s):  
Jain Deepak ◽  
Jain Anurekha
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Research Work ◽  
Radical Scavenging ◽  
Antioxidant Effect ◽  
Physical Parameters ◽  
Reference Standard ◽  
Phytochemical Screening ◽  
Polyherbal Formulation

Objective: The objective of this research was to develop a polyherbal formulation (PHF) using four different herbs and to evaluate their phytochemicals, physical constants, and determination of their antioxidant activity by DPPH method.Methods: The PHF authenticated herbs were characterized by studying its morphological and pharmacognostic characters. Phytochemical screening showed the presence of alkaloids, glycosides, carbohydrates, amino acid, tannin, steroids, and flavonoids in the combination extract. Physical parameters such as loss on drying (LOD), pH, ash values, LOD, and extractive value have been studied. The antioxidant activity of the combination of extract (100 mg each) was determined using DPPH free radical scavenging method.Results: The results showed that the combination extract has best antioxidant effect at a dose of 400 μg/ml when it was compared with ascorbic acid as the reference standard.Conclusion: The results obtained in this research work clearly showed that the combination extract has best antioxidant effect at a dose of 400 μg/ ml when it was compared with ascorbic acid as the reference standard. 

scholarly journals Determination of Antioxidants by Four Different Methods in Medicinally Important Plant Ebenus Stellata of Balochistan

2020 ◽  
Vol 23 (4) ◽  
pp. 13-18
Author(s):  
Aminullah Mandokhail ◽  
◽  
Samiullah Samiullah ◽  
Naqeebullah Khan ◽  
Abdul Hakeem Tareen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Acid Concentration ◽  
Frap Assay ◽  
Antioxidant Power ◽  
North West ◽  
The North ◽  
Total Antioxidant ◽  
High Antioxidant Activity

The quantification of a plant, which is vital in medication bearing antioxidant activity of the North West area of the province Balochistan Ebenus Stellata family Fabaceae is explained in this paper. The plant composition was determined chemically through well-known four analytical tests and sensitive EMR operating techniques. The crude extract was obtained from the plant by using methanol, and the antioxidant activity of that extract was measured. Additionally, with the help of spectrophotometer, antioxidant potentials were determined through the DPPH (2, 2-Diphenyl-1-Picrylhydrazyl) assay, Ferric ion (Fe+3) reducing antioxidant power assay, Ferric (Fe+3) reducing antioxidant potential (FRAP) assay, and total antioxidant capacity (TAC) of the phosphomolybdenum assay. The potency of the DPPH assay ofEbenus Stellatawas 179 mg/g ofascorbic acid concentration. Moreover, the FRAP and TAC values were 9.2mg and 35 mg of ascorbic acid/g ofEbenus stellate extract, respectively. Furthermore, RP denotes 2.6 mg/g of ascorbic acid concentration. The concerned plant showed high antioxidant activity.

scholarly journals Chemical Composition and Antioxidant Activity of Essential Oil of Sawdust from Moroccan Thuya (Tetraclinis articulata (Vahl) Masters

2020 ◽  
Vol 11 (1) ◽  
pp. 7912-7920
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Potential Source ◽  
Reduction Of Iron ◽  
Tetraclinis Articulata ◽  
Total Composition

The present study was dedicated to the study of the chemical composition and the antioxidant activity of the essential oils from the sawdust of Tetraclinis articulata in Morocco (from Khemisset region). The yield of the essential oil obtained by hydrodistillation from sawdust was 2.19%. The determination of the chemical composition of Tetraclinis articulata essential oils (TAEO) was carried out by GC-SM analysis. The results that 22 volatile compounds were identified in the sawdust part of T. articulata, representing 87.25% of the total composition. The study of TAEO antioxidant activity was assessed by three different methods: the trapping of free radical DPPH, bleaching assay ABTS, and the reduction of iron (FRAP). The results of DPPH test showed that TAEO (IC50=0.0144 mg/mL) has a very interesting antioxidant activity compared to the ascorbic acid used as reference (IC50=0.0184 mg/mL). These results reveal promising prospects for the future exploitation of TAEO as a potential source of natural antioxidant substances that may be used for future investigations.

scholarly journals Determination of amount of retinylpalmitate and ascorbic acid of extract gel of sweet corn fibre by UVand HPTLC method

2021 ◽  
Vol 6 (4) ◽  
pp. 201-204
Author(s):  
N Sunitha ◽  
S Manohar Babu ◽  
B Appa Rao
Keyword(s):  
Ascorbic Acid ◽  
Correlation Coefficient ◽  
Sweet Corn ◽  
Retinyl Palmitate ◽  
Laboratory Method ◽  
Corn Fibre ◽  
Dried Extract ◽  
Hptlc Method

Sweet corn fibres of about 15 gm were extracted with methanol for 5 hours in heating mantle at 40C and filtered and allowed to dry. The dried gel was further analyzed for estimation of Retinyl palmitate by spectrophotometrically by laboratory method and found to be 140 mg/kg. The dried extract gel was further estimated for ascorbic acid both by UV and HPTLC and found to be linear in the range of 1-5 ug/ml and 5-10ug/ml, correlation coefficient was found to be 0.997 and 0.998 and the amount of ascorbic acid was found to be 338 ng/ml and 9.9 ng/ml by UV and HPTLC respectively. The method was found to be linear.

Radical-Scavenging Compounds in Olive Fruits and their Changes during Table Olive Preparation

2013 ◽  
Vol 295-298 ◽  
pp. 118-122 ◽  
Author(s):  
Li Qin Jiang ◽  
Hitoshi Takamura
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Active Compounds ◽  
Table Olive ◽  
Radical Scavenging Compounds

The aim of this study was to determine the changes of antioxidant activity and antioxidative compounds during table olive preparing by different procedures. The extraction of antioxidants from table olive is studied considering different debittering procedure (alkali treated or brine treated) to investigate the selectivity of the process. The radical-scavenging activity (RSA) of 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and determination of the antioxidant compounds (total phenol, ascorbic acid and tocopherol) are applied to evaluate the antioxidant activity and antioxidative compounds of table olive. The active compounds and DPPH-RSA decreased during table olive preparation. A large amount of the active compounds lost during “alkali treated” except tocopherol. Little amount of the active compounds remained in “brine treated” table olive.

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