Effect of Black Grape Seed(Vitis Vinifera) Extract Consumption with Moderate-Intensity Aerobic Training on the Expression of MicroRNAs in Type 1 Diabetic Cardiac Tissue Rats

Objective: This study aimed to evaluate the effect of consuming grape seed extract with moderate-intensity aerobic training on the expression of miR-126 and miR-29 in the cardiac tissue in type 1 diabetic male rats. Materials and Methods: 40 rats with an initial weight range of 160-220 g were divided into five groups: Training + Extract, Training, Extract, Diabetic / Control, and Healthy / Control. Aerobic training program was moderate intensity and rats performed aerobic training for 60 minutes a day with the intensity 70 to 75% of maximum oxygen consumption (28 meters per minute). Grape seed extract was also administered by gavage at a dose of 40 mg/kg per day. Results: Expression of both miRNAs in the three groups of training + extract, healthy training and control was significantly higher than the two groups of extract and diabetic control (P-value= 0.001). The difference between the three groups of training + extract, healthy training and control and also the difference between the two groups of extract and diabetic control were not significant (P-value> 0.05). Conclusion: Aerobic training may be able to prevent cardiac disease caused by type 1 diabetes.

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The Aerobic Training and Berberine Chloride Intervention on Pancreatic Tissue Antioxidant Enzymes and Lipid Peroxidation in Type 1 Diabetic Rats

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v11i4.2882 ◽

2020 ◽

Author(s):

Javad Ramezani ◽

Mohammad Ali Azarbayjani ◽

Maghsoud Peeri

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Aerobic Training ◽

Diabetic Control ◽

Pancreatic Tissue ◽

Diabetic Rats ◽

Male Rats ◽

P Value ◽

Berberine Chloride

Objective: Aerobic training and berberine chloride include antioxidant characteristics. In this study, aerobic training and berberine chloride intervention on the activity of antioxidant enzymes and lipid peroxidation of pancreatic tissue were investigated in type 1 diabetic male wistar rats. Materials and Methods: In the current study, 56 Wistar male rats were randomly assigned to seven different groups (n= 8), these groups include healthy control, diabetic control, Berberine-treated diabetes (15&30 mg/kg), aerobic training-treated diabetes, Diabetes treated with Berberine and aerobic training (15&30 mg/kg). The aerobic training schedule consisted of 6 weeks treadmills with a frequency of 5 sessions per week. The Berberine was also fed a specific dose every day and a half before training. The superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde were assessed using ELISA method. Results: The results showed that aerobic training, as well as the intervention of Aerobic Training and Berberine chloride, had a significant effect on the increase of antioxidant enzymes SOD and CAT in the pancreatic tissue groups (P-value< 0.05), but did not significantly affect the GPX level (P-value> 0.05). There was a significant decrease in MDA level in all treatment groups (P-value< 0.05). In diabetic groups that received both treatments at the same time, the MDA level more decreased (P-value< 0.0005). Conclusion: The aerobic training and berberine chloride concurrent intervention have a greater effect on the antioxidant enzymes in the pancreatic tissue of diabetic specimens. Therefore, it is recommended that aerobic training be done with berberine chloride.

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The Effect of Aerobic Training on Tumor Necrosis Factor alpha, Hypoxia-Inducible Factor-1 alpha & Vascular Endothelial Growth Factor Gene Expression in Cardiac Tissue of Diabetic Rats

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v12i2.4047 ◽

2020 ◽

Author(s):

Fariba Bakhtiari ◽

Hasan Matin Homaee ◽

Farshad Ghazalian

Keyword(s):

Gene Expression ◽

Cardiac Tissue ◽

Aerobic Training ◽

Hypoxia Inducible Factor ◽

Diabetic Control ◽

Diabetic Rats ◽

P Value ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Endothelial Growth Factor

Objective: The goal of this research was to determine the influence of 4 weeks aerobic training on gene expression of tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in the cardiac tissue of diabetic rats. Materials and Methods: In an experimental study, 30 male wistar rats were partitioned into three groups (n=10), diabetic aerobic training, diabetic control and healthy control (n=10). Induction of type 2diabetes (T2DM) was done by intraperitoneal infusion of streptozotocin. The progressive aerobic training protocol entailed 4 weeks, 5 sessions per week running on treadmill at velocity of 20 m/min for 60 min. The gene expression of TNF-α, VEGF and HIF-1α were measured by real time & PCR. One way analysis of variance and bonferroni test were applied to analysis the data. The significant level was set at P-value< 0.05. Results: The results indicated that the aerobic training induced significant decrease in TNF-α mRNA (P-value< 0.001) and significant increase in HIF-1α mRNA (P-value< 0.001) and VEGF mRNA (P-value< 0.001) compared to diabetic control group. Conclusion: It appears that aerobic training with reduction of TNF-α issues an affirmative effect on angiogenesis, as a result, it improves diabetic cardiac.

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Cardiomyopathy in Offspring of Pregestational Diabetic Mouse Pregnancy

Journal of Diabetes Research ◽

10.1155/2014/624939 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

Daniel Dowling ◽

Niamh Corrigan ◽

Stephen Horgan ◽

Chris J. Watson ◽

John Baugh ◽

...

Keyword(s):

Cardiac Tissue ◽

Diabetic Mouse ◽

Late Gestation ◽

Collagen Deposition ◽

Diabetic Mice ◽

Intrauterine Environment ◽

And Control ◽

Picrosirius Red ◽

Myocardial Collagen

Purpose. To investigate cardiomyopathy in offspring in a mouse model of pregestational type 1 diabetic pregnancy.Methods. Pregestational diabetes was induced with STZ administration in female C57BL6/J mice that were subsequently mated with healthy C57BL6/J males. Offspring were sacrificed at embryonic day 18.5 and 6-week adolescent and 12-week adult stages. The size and number of cardiomyocyte nuclei and also the extent of collagen deposition within the hearts of diabetic and control offspring were assessed following cardiac tissue staining with either haematoxylin and eosin or Picrosirius red and subsequently quantified using automated digital image analysis.Results. Offspring from diabetic mice at embryonic day 18.5 had a significantly higher number of cardiomyocyte nuclei present compared to controls. These nuclei were also significantly smaller than controls. Collagen deposition was shown to be significantly increased in the hearts of diabetic offspring at the same age. No significant differences were found between the groups at 6 and 12 weeks.Conclusions. Our results from offspring of type 1 diabetic mice show increased myocardial collagen deposition in late gestation and have increased myocardial nuclear counts (hyperplasia) as opposed to increased myocardial nuclear size (hypertrophy) in late gestation. These changes normalize postpartum after removal from the maternal intrauterine environment.

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SERUM MAGNESIUM LEVEL IN DIABETIC RETINOPATHY - A CROSS SECTIONAL STUDY

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i5.257 ◽

2019 ◽

Vol 3 (5) ◽

Author(s):

Dr. I. Vijayapriya ◽

Dr. Prakash. S ◽

Dr. S. Hemadharshini

Keyword(s):

Type 2 Diabetes ◽

Diabetic Retinopathy ◽

Serum Magnesium ◽

Study Groups ◽

Control Group ◽

P Value ◽

Cross Sectional ◽

The Difference ◽

And Control

Background: Among different complications of diabetes, ddiabetic retinopathy is one of the leading causes of blindness in the world. Increase in the frequency of lower serum magnesium levels have been reported among patients with diabetes. Materials and methods: A total of 120 subjects were included in this study and divided into 3 groups. The study groups consisted of 40 patients that are type 2 diabetes with retinopathy and 40 patients with type 2 diabetes without retinopathy and control group consisted of 40 healthy subjects respectively. Both cases and controls were subjected to estimation of biochemical parameters. Results: Among the study population, (80) 66.67% participants were cases and another (40) 33.33% participants were controls. Among the people who had mild NPDR, the median Mg was 1.90 (IQR 1.80, 2.00). It was 1.90 (1.70, 2.00), 1.75 (1.67, 1.92), 1.8 (1.69, 2.0) and 2.10 (1.90, 2.20) among people with DM retinopathy moderate NPDR, severe NPDR, Proliferative retinopathy and no retinopathy respectively. The difference in the Mg across DM retinopathy was statistically significant (P Value 0.008). The difference between the values among both the case and control groups for certain parameters such as SBP, FBG, PPBG, HbA1c, Magnesium, Urea, and Creatinine were found to be statistically significant. Conclusion: Serum magnesium levels were significantly lower in patients with diabetic retinopathy compared to diabetics without complications and control group. Keywords: Diabetes mellitus, Diabetic retinopathy, Magnesium

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PENERAPAN BIRTH BALL DENGAN TEKNIK PELVIC ROCKING TERHADAP LAMA PERSALINAN PADA KALA I FASE AKTIF

JURNAL KEPERAWATAN DAN FISIOTERAPI (JKF) ◽

10.35451/jkf.v4i1.837 ◽

2021 ◽

Vol 4 (1) ◽

pp. 70-76

Author(s):

Diah Evawanna Anuhgera ◽

Nikmah Jalilah Ritonga ◽

Riris Sitorus ◽

Juni Mariati Simarmata

Keyword(s):

Active Phase ◽

Control Group ◽

P Value ◽

Control Groups ◽

Midwifery Care ◽

Quasi Experimental ◽

Review Study ◽

The Difference ◽

And Control ◽

Measured Duration

A comfortable childbirth is the dream of mothers. Faster delivery progress can lead to quality midwifery care. Birth ball is a tool that can be used by mothers to position the pelvis properly, especially applied the pelvic rocking technique. This review study aimed to evaluate the evidence regarding the effect of using birth ball with pelvic rocking in duration of labor in kala I active phase. This study followed a quasi experimental research design with posttest control group design.The sample consisted of 12 treatment people dan 12 controls with the purposive sampling.Partograph was measured duration of labor in kala I active phase. The difference in duration of labor for the treatment and control group were tested with Mann Whitney. There was a difference in the effect of using a birth ball with pelvic rocking technique in length of labor in kala I active phase in the treatment and control groups with p-value 0.001. The use of birth ball with pelvic rocking technique is evidence to speed the duration of labor in kala I active phase. Birth ball with pelvic rocking are recommended to be use an alternative to speed the duration of labor in kala I active phase in midwifery services

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EVALUATING OF EFFERVESCENT TABLETS CONTAINING GRAPE SEED (VITIS VINIFERA L.) EXTRACT AS A NUTRACEUTICAL

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s1.76_83 ◽

2017 ◽

Vol 9 ◽

pp. 150

Author(s):

Silvia Surini ◽

Mutiah Rakhma Wisnu Wardani ◽

Erny Sagita

Keyword(s):

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Vitis Vinifera L ◽

Total Phenol Content ◽

Wet Granulation ◽

Disintegration Time ◽

Acceptable Range ◽

And Control ◽

Equilibrium Vapor Pressure

Objective: Grape is one of the most well-known fruits. People usually consume only the fruit and the skin; however, the seed is the part of the fruitthat contains important antioxidant rich polyphenol. However, grape seed and its extract have an unpleasant taste. Therefore, this study aimed toformulate effervescent tablets containing grape seed extract (GSE) to overcome the unpleasant taste.Methods: Effervescent tablets of GSE were formulated using three formulas, each with a different percentage of the effervescent mix. The tablets wereprepared using wet granulation method at 40% relative humidity (RH) (the ratio of the partial pressure of water vapor to the equilibrium vapor pressure ofwater) and 25°C temperature. The effervescent granules and the tablets were evaluated for various characteristics in term of granules flowability, moisturecontent, as well as tablets appearance, size and weight uniformity, hardness, friability, effervescence time, pH, and total phenol content. In addition, all threeformulations of the effervescent tablets and solutions were evaluated for appearance, taste, and flavor using the hedonic test that involved 30 panelists.Results: The evaluation of the effervescent granules and tablets showed that they had good characteristics. The disintegration time of the threeformulations was within the acceptable range, between 3.67 minutes and 4.69 minutes. The pH of the effervescent solution was between 5.18 and5.80. Based on the hedonic test, all the effervescent solutions had favorable appearance, taste, and flavor.Conclusions: Clinical Streptococcus salivarius isolates from the dorsum of the tongue had greater potential for inhibiting Enterococcus faecalis growthcompared to the saliva isolates and control bacteria. Therefore, we can conclude that the effervescent tablets containing grape seed extract arepotential be used as a nutraceutical dosage form.

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Pengaruh Konsumsi Tempe terhadap Kadar Gula darah pada Penderita Diabetes Melitus di Kelompok Senam Ibu-Ibu di Kelurahan Talang Banjar Jambi

Jurnal Akademika Baiturrahim Jambi ◽

10.36565/jab.v9i1.195 ◽

2020 ◽

Vol 9 (1) ◽

pp. 110

Author(s):

Iin Indrawati ◽

Maimaznah Maimaznah

Keyword(s):

Diabetes Mellitus ◽

Blood Sugar ◽

Eating Habits ◽

The Body ◽

P Value ◽

Control Programs ◽

Quasi Experimental ◽

Sugar Levels ◽

And Control

DM management that is more effective and efficient requires prevention and control programs that are on target. There are several risk factors associated with the incidence of DM, one of which is a behavioral factor. The behavior or lifestyle in question is eating habits. The purpose of this study was to determine the effect of tempe consumption on blood sugar levels of DM sufferers in the exercise groups of women in the Talang Banjar Village, Jambi.The research method used was a quasi-experimental one group pre and post experience test. The population is group of gymnastics mothers of gymnastics mothers who suffer from diabetes mellitus, amounting to 6 people, using total sampling techniques, and analyzed using T Test.The analysis showed that there was no effect of tempe consumption with blood sugar levels with a p value (p value 0.201).Although the results of the study did not show any influence, it is likely that this was caused by several things such as the small number of samples, and it was not determined whether the N sample was a type 1 or type 2 diabetes sufferer. diabetes mellitus as a controller or helps control blood sugar levels in the body.

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The Effect of Aerobic Training and L- Carnitine on BCL2 and Mitochondrial Enzymes of Rat's Kidney

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v11i2.2663 ◽

2020 ◽

Author(s):

Ali Fakourian ◽

Hasan ◽

Mohammad Ali Azarbayjani ◽

Parvin Farzanegi

Keyword(s):

Aerobic Exercise ◽

Aerobic Training ◽

Kidney Tissue ◽

Diabetic Control ◽

Diabetic Rats ◽

Combined Effect ◽

Diabetic Group ◽

Control Group ◽

P Value ◽

Mitochondrial Enzymes

Objective: Diabetes causes free radical production, oxidative stress and alterations in mitochondrial enzymes and apoptosis. The purpose of this study was to investigate the effect of aerobic exercise and consumption of L-carnitine on BCL2 and some mitochondrial enzymes of the kidney in diabetic rats. Materials and Methods: In this experimental study, 45 male wistar rats (200-300 gr) were simple randomly divided into six groups: 1) placebo group, 2) healthy control group, 3) diabetic control group, 4) diabetic group receiving L- carnitine, 5) diabetic group of aerobic training and 6) diabetic group of aerobic training and recipient of L-carnitine. Rats with a serum glucose level higher than 300 mg/ dL were considered diabetic. L-carnitine recipients received 100 mg of L-carnitine daily orally. The dependent variables of the study were measured 24 hours after the last training program session by ELISA in kidney tissue. Data were analyzed by Shapiro- Wilk and two way ANOVA and Tukey post hoc test at P -value< 0.05. Results: The results showed that the combined effect of aerobic training and supplementation of L-carnitine on Bcl2 factor of kidney tissue of rats with diabetes has a significant effect ( P -value: 0.019). But aerobic exercise ( P -value: 0.969) and supplementation of Lcarnitine ( P -value: 0.584) were not significant. Conclusion: The results of this study showed that the combined effect of aerobic exercise and supplementation of L-carnitine on CPT2 and Malonyl COa and Bcl2 have a significant effect on the kidney of diabetic rats. Also, exercise alone and consumption of Lcarnitine alone do not have a significant effect.

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The Effect of Continuous Aerobic Training on Bax/Bcl2 Ratio in Pancreatic Tissue Diabetic Rats

Iranian journal of diabetes and obesity ◽

10.18502/ijdo.v13i3.7192 ◽

2021 ◽

Author(s):

Mehdi Bostani ◽

Seyed Aenollah Noaein

Keyword(s):

Effect Size ◽

Pancreatic Beta Cells ◽

Aerobic Training ◽

Diabetic Control ◽

Pancreatic Tissue ◽

Diabetic Rats ◽

Aerobic Exercise Training ◽

P Value ◽

Bax Protein ◽

Male Wistar Rats

Objective: Apoptosis of pancreatic beta cells plays an important role in the pathogenesis of type 1 diabetes. the purpose of this study was to investigate the effect of continuous aerobic training on Bax/Bcl-2 ratio in pancreatic tissue of streptozotocin (STZ)-induced diabetic rats. Materials and Methods: A total number of 40 male Wistar rats, were divided into healthy control (HC), healthy trained (HT), diabetic control (DC), and diabetic trained (DT) groups. Diabetes was also induced by a single intraperitoneally injection of STZ (45 mg/kg). the training groups performed the exercise on the treadmill for five consecutive days within six weeks with 10-18 m/min intensity and 10-30 minute duration based on the principle of overload. the pancreatic tissue levels of the Bax and the Bcl-2 proteins were further determined via the ELISA method. The analysis of variance test (ANOVA) with Tukey's post hoc test was used for analyzing the data. Results: The results showed that the induction of diabetes had significantly decreased the levels of Bcl-2 protein and increased the levels of Bax protein and Bax/Bcl-2 ratio in the pancreatic tissue (Pvalue=0.001,Effect Size=0.29). As well, the findings showed that six weeks of aerobic exercise training had significantly increased the levels of Bcl-2 and significantly decreased the levels of Bax and Bax/Bcl-2 ratio in the DT group (P-value=0.012, Effect Size=0.23). Conclusion: According to the results of this study, exercise can be considered an effective strategy to reduce the rate of diabeticinduced apoptosis and control its complications.

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Effect of the Use Lethal Ovitrap to Population Aedes sp

Medical Science & Healthcare Practice ◽

10.22158/mshp.v1n1p20 ◽

2017 ◽

Vol 1 (1) ◽

pp. 20

Author(s):

Tri Ramadhani ◽

Bondan Fajar Wahyudi

Keyword(s):

Population Control ◽

Total Sample ◽

Control Group ◽

P Value ◽

Before And After ◽

Quasi Experimental ◽

The Mean ◽

The Difference ◽

And Control ◽

Experimental Group

Dengue vector control has been done in various ways, however, has not been able to obtain optimal results. Ovirap use to determine the presence of Aedes sp in a region is often done, but the application of lethal ovitrap for population control Aedes sp still rarely done. This study was aimed to assess the effect of LO applications on populations of Aedes sp. This research includes quasi experimental design with a pretest-posttest control group without randomization. Research sites in dengue in endemic areas in Banyumas district, with a total sample of 100 homes in each treatment and control areas. Insecticides used on ovistrip is cypermethrin at a dose of 12.5 mg ai/strip. Populations of Aedes sp measured each week for three weeks prior to the intervention and twelve weeks during the intervention. The mean density of Aedes sp compared before and after intervention and between treatment and control areas. The study showed the difference of mosquito densities before and after the intervention in the experimental group was 0,07 ( p-value 0.044), whereas the control group was 0.037 (p-value 0.341). LO use with the addition of insecticide active ingredient cypermetrin on ovistrip impact on the decline density of Aedes sp.

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