scholarly journals Edible films based on chitosan-flaxseed mucilage: in vitro antimicrobial and antioxidant properties and their application on survival of food-borne pathogenic bacteria in raw minced trout fillets

2019 ◽  
Author(s):  
Negin Karami ◽  
Abolfazl Kamkar ◽  
Yasser Shahbazi ◽  
Ali Misaghi
Keyword(s):  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Edible Films ◽  
Diffusion Method ◽  
E Coli ◽  
Natural Oils ◽  
Food Borne

Various natural oils/extracts and their constituents incorporated into biopolymer-based edible films as a promising technology with the knowledge that these compounds have been able to reduce microbial growth and chemical changes of packed foodstuffs. The objective of this study was to evaluate the effect of incorporation of Ziziphora clinopodioides essential oil (ZEO; 0, 0.25 and 0.5%) and sesame oil (SO; 0, 0.5 and 0.75%) into chitosan-flaxseed mucilage (CH-FM) film against Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus and Escherichia coli O157:H7 in vitro condition and raw minced trout fillets during refrigerated condition. The in vitro antibacterial and antioxidant properties of CH-FM films were evaluated using agar disk diffusion method and free radical scavenging activity assay, respectively. The most important constituents of ZEO were found to be carvacrol (65.22%), thymol (19.51%), ɣ-terpinene (4.63%) and p-cymene (4.86%). The lowest and highest antimicrobial effect against S. aureus, L. monocytogenes, E. coli O157:H7 and S. typhimurium were found for CH-FM films enriched with SO 0.5% (0.98-1.24 mm) and ZEO 0.5% + SO 0.75% (5.01-6.25 mm), respectively. The antioxidant property of CH-FM based films were found to be ranged 5.45% ± 0.04-37% ± 0.45. In treated trout fillets, the counts of L. monocytogenes, S. aureus, E. coli O157:H7 and S. typhimurium were 1.54-4.18, 0.34-3.35, 0.29-1.45 and 0.19-1.27 log CFU/g significantly lower than control groups after two weeks of refrigerated storage, respectively. The designated films had good antibacterial effect against some food borne pathogenic bacteria including L. monocytogenes, S. aureus, S. typhimurium and E. coli O157:H7 in raw rainbow trout fillets.

Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Diffusion Method ◽  
Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

scholarly journals Study of Antimicrobial, Antioxidant and Cytotoxicity Properties of Selected Plant Extracts for Food Preservative Applications

2021 ◽  
pp. 95-111
Author(s):  
Tania Islam ◽  
Md Nazrul Islam ◽  
Wahidu Zzaman ◽  
Md Morsaline Billah
Keyword(s):  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
In Vivo Studies ◽  
Diffusion Method ◽  
Plant Materials ◽  
Food Preservative

An attempt has been made to evaluate antimicrobial, antioxidant and cytotoxicity properties of extracts from onion (Allium cepa L.), garlic (Allium sativum), leaves of guava (Psidium guajava), papaya (Carica papaya), tea (Camellia sinensis), baen (Avicennia alba) and keora (Sonneratia apetala), respectively to apply as natural preservatives for tomatoes. The air-dried plant materials of the respective plant species were subjected to ethanol-methanol extraction, concentrated and stored at 4 °C before use. The extracts were dissolved in 95% ethanol for analysis of antioxidant and antimicrobial properties. Of the extracts tested, tea extracts showed the highest zone of inhibition against several pathogenic bacteria (E. coli 35.0±3.2 mm; P. aeruginosa 29.3±2.6 mm; S. typhi 28.4±2.1 mm and S. pyogenes 27.7±3.7 mm) using the disc diffusion method. In regard to DPPH free radical scavenging assay, keora and guava extracts showed the highest percentage of radical scavenging activity with the values of 89.64± 0.18 and 89.39± 0.88, respectively, which were in agreement with higher total antioxidant capacity (TAC) of these extracts obtained by the phosphomolybdenum method. Brine shrimp lethality bioassay for cytotoxicity assessment showed LC50 of 132.54 ± 18.99 µg/mL for the leaf extract of keora which was found to be most toxic among all studied extracts. The initial results indicated that the extracts could be used for food preservative applications based on the antimicrobial, antioxidant and cytotoxicity properties of the tested extracts. However, efficacy, stability and safety issues need to be addressed with both in vitro and in vivo studies.

scholarly journals EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ISOLATED CONSTITUENTS FROM AREAL PART OF CUSCUTA REFLEXA ROXB. PLANT

2018 ◽  
Vol 11 (3) ◽  
pp. 187
Author(s):  
Sandeep Sandeep ◽  
Abhilasha Mittal ◽  
Abhilasha Mittal
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
E Coli ◽  
Cuscuta Reflexa ◽  
Isolated Compound ◽  
Antimicrobial And Antioxidant Activity ◽  
Dpph Radical Scavenging

 Objective: The objective of the study was to investigate the pharmacological evaluation of previously isolated compounds (CR-1 to CR-5) from the areal part of Cuscuta reflexa Roxb. is reported.Methods: The antimicrobial and antioxidant activity of the isolated compounds (CR-1 to CR-5) from C. reflexa was determined by the disc-diffusion method and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) model, respectively. The antimicrobial activity was performed against four strains Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa.Results: The results revealed that highest zone of inhibition is measured by compound CR-5 against E. coli. The antioxidant activity is evaluated for in vitro antioxidant activity using DPPH radical scavenging activity, inhibitory concentration 50% (IC50) (120.92–76.38 %), respectively. The results indicate that isolated compound CR-1 and CR-2 having IC50 76.38 and 76.94 μg/ml, respectively, showed potent antioxidant activity comparable to standard ascorbic acid (IC50 43.42 μg/ml).Conclusion: This study suggests that areal part of C. reflexa have bioactive compounds for a new antimicrobial and antioxidant drug development.

scholarly journals Thymoquinone-loaded PLGA nanoparticles: antioxidant and anti-microbial properties

2013 ◽  
Vol 2 (12) ◽  
pp. 202-207 ◽  
Author(s):  
Ilaiyaraja Nallamuthu ◽  
Ambica Parthasarathi ◽  
Farhath Khanum
Keyword(s):  
Nigella Sativa ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Pharmaceutical Journal ◽  
Salmonella Typhi ◽  
Plga Nanoparticles ◽  
Diffusion Method ◽  
E Coli ◽  
Oil In Water

The aim of the present study was to synthesize and characterize the Thymoquinone (TQ) encapsulated PLGA (poly (dl-lactide-co-glycolide) nanoparticles, and further evaluate for its antioxidant and anti-bacterial activities. TQ is a potential active ingredient of Nigella sativa seed and possess a spectrum of therapeutic properties. Nanoparticles were prepared according to solid-in-oil-in-water (s/o/w) solvent evaporation method. Dynamic laser light scattering (DLS) and SEM studies indicated a mean particle size of < 200 nm. The success of encapsulation was confirmed by FTIR technique, and the encapsulation efficiency (EE) of TQ was determined to be 62%. In vitro drug release study showed a maximum release of TQ at 75% and 54 % respectively for artificial intestinal and gastric juices over the period of 7 days. DPPH radical scavenging activity of the nanoparticles was found to be 71% at 1 mg/ml concentration. It also exhibited antibacterial property against E. coli, Staphylococcus aureus and Salmonella typhi strains, tested using well diffusion method. In conclusion, our study shows that PLGA encapsulated TQ nanoparticle with sustained release property has preserved antioxidant as well as anti-microbial activity, and therefore suggesting its therapeutic applications in various food samples.DOI: http://dx.doi.org/10.3329/icpj.v2i12.17017 International Current Pharmaceutical Journal, November 2013, 2(12): 202-207

scholarly journals Synthesis, Molecular Docking Analysis, and Evaluation of Antibacterial and Antioxidant Properties of Stilbenes and Pinacol of Quinolines

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Zeleke Digafie ◽  
Yadessa Melaku ◽  
Zerihun Belay ◽  
Rajalakshmanan Eswaramoorthy
Keyword(s):  
Molecular Docking ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Positive Control ◽  
Health Concern ◽  
Diffusion Method ◽  
E Coli

Emergence of antimicrobial resistance to standard commercial drugs has become a critical public health concern worldwide. Hence, novel antimicrobials with improved biological activities are urgently needed. In this regard, a series of quinoline-stilbene derivatives were synthesized from substituted quinoline and benzyltriphenylphosphonium chloride using Wittig reaction. Furthermore, a novel pinacol of quinoline was synthesized by pinacolinazation of 2-methoxyquinoline-3-carbaldehyde which was achieved by aluminum powder-potassium hydroxide reagent combination at ambient temperature in methanol. The structures of the synthesized compounds were established based on their spectral data. The antibacterial activities of the synthesized compounds were evaluated in vitro by the paper disc diffusion method against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Escherichia coli and Salmonella typhimurium). The best activity was displayed by compound 19 against E. coli with an inhibition zone of 16.0 ± 0.82 mm and 14.67 ± 0.94 mm at 500 and 250 μg/mL, respectively. This is close to ciprofloxacin which is used as a positive control. The results of in silico molecular docking evaluation of the compounds against E. coli DNA gyraseB were in good agreement with the in vitro antibacterial analysis. Compounds 19 (−6.9 kcal/mol) and 24 (−7.1 kcal/mol) showed the maximum binding affinity close to ciprofloxacin (−7.3 kcal/mol) used as positive control. Therefore, the antibacterial activity displayed by these compounds is encouraging for further investigation to improve the activities of quinoline-stilbenes by incorporating various bioisosteric groups in one or more positions of the phenyl nuclei for their potential pharmacological use. Findings of the DPPH radical scavenging assay indicated that some of the quinolone stilbenes and pinacol possess moderate antioxidant properties compared to ascorbic acid used as a natural antioxidant.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF SAPONIN FROM ABUTILON INDICUM LEAVES

2016 ◽  
Vol 9 (9) ◽  
pp. 344 ◽  
Author(s):  
Lokesh Ravi ◽  
Manasvi V ◽  
Praveena Lakshmi B
Keyword(s):  
Escherichia Coli ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
E Coli ◽  
Ms Analysis ◽  
Antibacterial Assay ◽  
Crude Saponin ◽  
Abutilon Indicum

ABSTRACTObjective: Aim of this study is to analyze the antibacterial and antioxidant potential of crude saponin extract (CSE) from Abutilon indicum leaves.Methods: CSE was subjected for gas chromatography-mass spectrometry (GC-MS) analysis to identify its components. Antibacterial potentialwas analyzed using agar well diffusion method and minimum inhibitory concentration (MIC) was detected using 96-well plate method, againstStaphylococcus aureus (MTCC: 3160) and Escherichia coli (MTCC: 443). DNA damage study was performed using comet assay. Antioxidant capabilitywas studied using 2,2-diphenyl-1-picrylhydrazyl scavenging assay.Results: GC-MS analysis suggested a library match to benzene-1-4-bis(phenylmethyl), with a molecular weight of 258 g/mol to be the majorcomponent in the CSE at 21.25 RT. CSE demonstrated 96.16% free radical scavenging activity at 2.5 mg/ml concentration. CSE demonstrateda significant antibacterial activity in the well diffusion assay, S. aureus 17 mm and E. coli 15 mm, with a MIC value of 1.11 mg/ml. Comet assaydemonstrated no DNA damage.Conclusion: These results conclude that CSE of A. indicum leaves possesses promising antibacterial and antioxidant potential.Keywords: Abutilon indicum, Saponin, Escherichia coli, Staphylococcus aureus, 2,2-diphenyl-1-picrylhydrazyl, Antibacterial assay.

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

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