scholarly journals High Expression of FcγII (CD32) Receptor on Monocytes in Dengue Infected Patients

2018 ◽  
Vol 10 (3) ◽  
pp. 256-62
Author(s):  
Umi Solekhah Intansari ◽  
Harina Salim ◽  
Usi Sukorini ◽  
Adika Zhulhi Arjana ◽  
Muhammad Juffrie
Keyword(s):  
Flow Cytometry ◽  
Dengue Virus ◽  
Healthy Subjects ◽  
Dengue Infection ◽  
Absolute Number ◽  
Severe Dengue ◽  
Cross Sectional ◽  
Fluorescent Intensity ◽  
The Mean ◽  
Cross Sectional Design

BACKGROUND: Pathogenesis of severe dengue infection has not been elucidated. Immune complex of pre-existing antibodies and heterotypic dengue virus bind to FcγII (cluster of differentiation (CD32)) receptor (FcγIIR) on monocyte facilitates entry and replication of dengue virus. Aim of this study was to evaluate the expression of FcγIIR on monocytes in patients infected with dengue and in healthy subjects.METHODS: This study used a cross-sectional design that included patients infected with dengue who were hospitalized in Dr. Sardjito General Hospital, Panembahan Senopati Hospital, and Sleman Hospital, who met the inclusion criteria and selected consecutively. Examinations were completed using a lyse, no-wash method of flow cytometry. Computerized statistical analysis was conducted and was considered to be significant if p<0.05.RESULTS: Sixty-five study subjects were divided into healthy subjects (24 subjects) and patients with dengue infection (41 subjects). There were no significant differences in hemoglobin (Hb) and hematocrit (Hct) values between the groups, but differences were found in the number of leukocytes, absolute number of monocytes and platelet count (p<0.001, 0.002 and <0.001, respectively). The mean expression of FcγIIR monocytes in patients with dengue infection (208.77±32.06 median fluorescent intensity (MFI)) and the healthy subjects (124.03±47.76 MFI) with p<0.0001.CONCLUSION: The mean expression of FcγIIR monocytes in patients with dengue infection was higher than in healthy subjects.KEYWORDS: dengue infection, FcγII (CD32) receptor monocyte, flow cytometry

scholarly journals Liver function tests to predict the severity of dengue fever in serologically positive children below 18 years of age

2019 ◽  
Vol 6 (2) ◽  
pp. 358
Author(s):  
Yashwanth Raju H. N. ◽  
Rajendra Kumar ◽  
Sudha Rudrappa
Keyword(s):  
Serum Albumin ◽  
Dengue Fever ◽  
Hepatic Dysfunction ◽  
Dengue Infection ◽  
Liver Function Tests ◽  
Relevant Information ◽  
Warning Signs ◽  
Severe Dengue ◽  
Cross Sectional ◽  
The Mean

Background: Dengue fever presents with a diverse clinical spectrum. Although liver is not a major target organ, hepatic dysfunction is a well-recognized feature. In this study authors attempted to study the pattern of hepatic involvement in children with dengue and its association with disease severity.Methods: This was a cross sectional study conducted at Cheluvamba hospital during the period of 1 year. Children <18 years of age with dengue Ns1 Ag and IgM positive were included in this study. After obtaining informed consent, a pre-structured proforma was used to record the relevant information from each subject. After detailed clinical examination and haematological investigation children were categorized into three groups as dengue fever with no warning signs (DNWS), dengue fever with warning signs (DWWS) and severe dengue fever (SDF) according to WHO classification. Statistical analysis was done to know the strength of association between different clinical and biochemical variables and outcome of the disease.Results: The mean age of the study population was 8.65 years with male preponderance. The mean total bilirubin, serum albumin, SGOT, SGPT, ALP, PT and INR were 0.76 mg/dl, 3.8g/dl, 233.18U/L, 118.15U/L, 200.65 U/L, 12.9s and 1.09 respectively. The mean SGOT was significantly higher than SGPT. The degree of deranged LFTs was significantly more in SDF group than DNWS and DWWS groups. Serum albumin was significantly decreased in children with SDF group correlating with disease severity, prognosis and outcome.Conclusions: Hepatic dysfunction was present in all forms of dengue infection, with SGOT rising significantly more than SGPT. All biochemical liver parameters were significantly deranged in patients with severe dengue fever indicating prolonged illness and poor prognosis.

scholarly journals Geographical distribution and spatio-temporal patterns of hospitalization due to dengue infection at a leading specialist hospital in Malaysia

2018 ◽  
Vol 13 (1) ◽  
Author(s):  
Gary K.K. Low ◽  
Panayoti Papapreponis ◽  
Ridzuan M. Isa ◽  
Seng Chiew Gan ◽  
Hui Yee Chee ◽  
...  
Keyword(s):  
Dengue Infection ◽  
Geographic Location ◽  
Cross Sectional Study ◽  
Severe Dengue ◽  
Standardized Incidence Ratio ◽  
Cross Sectional ◽  
Secondary Objective ◽  
The Mean ◽  
Spatio Temporal ◽  
Age Range

Increasing numbers of dengue infection worldwide have led to a rise in deaths due to complications caused by this disease. We present here a cross-sectional study of dengue patients who attended the Emergency and Trauma Department of Ampang Hospital, one of Malaysia’s leading specialist hospitals. The objective was to search for potential clustering of severe dengue, in space and/or time, among the annual admissions with the secondary objective to describe the spatio-temporal pattern of all dengue cases admitted to this hospital. The dengue status of the patients was confirmed serologically with the geographic location of the patients determined by residency, but not more specific than the street level. A total of 1165 dengue patients were included in the analysis using SaTScan software. The mean age of these patients was 27.8 years, with a standard deviation of 14.2 years and an age range from 1 to 77 years, among whom 54 (4.6%) were cases of severe dengue. A cluster of general dengue cases was identified occurring from October to December in the study year of 2015 but the inclusion of severe dengue in that cluster was not statistically significant (P=0.862). The standardized incidence ratio was 1.51. General presence of dengue cases was, however, detected to be concentrated at the end of the year, which should be useful for hospital planning and management if this pattern holds.

scholarly journals KARAKTERISTIK ERITROSIT PADA PASIEN ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Indah R. A. Kewo ◽  
Glady Rambert ◽  
Firginia Manoppo
Keyword(s):  
Dengue Virus ◽  
Dengue Infection ◽  
Erythrocyte Count ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Viral Infectious Disease ◽  
Hematological Abnormalities ◽  
Characteristic Features ◽  
Cross Sectional Design ◽  
Normal Erythrocyte

Abstract: Dengue infection is a kind of viral infectious disease that can cause death. There are several hematological abnormalities frequently found in dengue infection, as follows: leukopenia, thrombocytopenia, coagulation disorders, bone marrow suppression, as well as increases in hemoglobin level and hematocrit value due to leakage of plasma. This study aimed to obtain the characteristic features of erythrocytes in children infected with dengue virus in Manado. This study used a cross sectional design and was conducted from December 2014 until January 2015 at Pancaran Kasih GMIM Hospital, Wolter Monginsidi Hospital, and Advent Hospital in Manado. Examination of the characteristics of erythrocytes was conducted at Prokita Malalayang Laboratory. Samples were 37 patients. The results showed that low erythrocyte count was found in 2 patients (5.41%); normal erythrocyte count in 34 patients (91.9%); and increased erythrocyte count in 1 patient (2.7%). Low mean copuscular volume (MCV) (MCHC) was found in 35 patients (94.6%), and increased MCHC in 2 patients (5.4%). Conclusion: Most children with dengue viral infection in Manado had normal erythrocyte count, along with MCV and MCHC in the range of the reference values (normocytic and normochromic).Keywords: Dengue virus infection, the number of erythrocytes, MCV, MCHC.Abstrak: Infeksi virus dengue merupakan penyakit infeksi yang dapat menyebabkan kematian. Kelainan hematologi sering muncul pada demam berdarah dengue diantaranya leukopenia, trombositopenia, gangguan koagulasi, penekanan sumsum tulang, juga terjadi peningkatan kadar hemoglobin dan nilai hematokrit karena kebocoran plasma yang dapat menyebabkan kematian. Penelitian ini bertujuan untuk mengetahui karakteristik eritrosit pada anak yang terinfeksi virus dengue di Manado. Desain penelitian potong lintang dengan jumlah sampel 37 pasien. Penelitian ini dilaksanakan di RSU Pancaran Kasih GMIM Manado, RS Wolter Mongisidi Manado, dan RS Advent Manado pada bulan Desember 2014 sampai Januari 2015. Pemeriksaan karakteristik eritrosit dilakukan di Laboratorium Prokita Malalayang. Hasil penelitian memperlihatkan jumlah eritrosit rendah ditemukan pada 2 orang (5,41%); normal 34 orang (91,9%); dan meningkat 1 orang (2,7%). Nilai mean copuscular volume (MCV) rendah pada 8 orang (21,6%); normal 28 orang (75,7%); dan meningkat 1 orang (2,7%). Nilai mean copuscular hemoglobin consentration (MCHC) normal pada 35 orang (94,6%), dan meningkat 2 orang (5,4%). Simpulan: Pada sebagian besar pasien anak dengan infeksi virus dengue di Manado didapatkan jumlah eritrosit dalam batas nilai normal, serta nilai MCV dan MCHC berada diantara rentang nilai rujukan (normositik dan normokromik).Kata kunci: infeksi virus dengue, eritrosit, MCV, MCHC

scholarly journals PENGUKURAN OVITRAP INDEX (OI) SEBAGAI GAMBARAN KEPADATAN NYAMUK DI DAERAH ENDEMIS DEMAM BERDARAH DENGUE (DBD) KABUPATEN BANYUMAS

2017 ◽  
Vol 9 (1) ◽  
pp. 56 ◽  
Author(s):  
Siwi Pramatama Mars Wijayanti ◽  
Dian Anandari ◽  
Arrum Firda Ayu Maghfiroh
Keyword(s):  
Dengue Virus ◽  
Community Health ◽  
Health Center ◽  
Community Health Center ◽  
Mosquito Density ◽  
Cross Sectional ◽  
Breeding Sites ◽  
Cross Sectional Design ◽  
Area Of Study

Mosquito density could predict the risk of Dengue Virus (DENV) transmission in certain area. Ovitrap Index (OI) is one of the methods to measure the mosquito density beside other methods such as Stegomyia Indices and free larvae index. Ovitrap index is cheaper, more appliacable and sensitive to detect Aedes sp activity to lay their eggs on the wall of container. The aims of this research were to measure ovitrap index in endemic DENV areas, to find out the preferences of female Aedes sp to lay their eggs, and find out the correlation between OI and dengue cases in endemic DENV areas. Type of this study was decriptive with cross sectional design. The area of study was in Community Health center Purwokerto Timur II in Kranji, Sokanegara and Purwokerto Lor. Ovitrap were installed at 50 houses of each vilagges (one indoor, one outdoor), with total ovitrap 100 in each villages. After 6 days of installation, ovistrips were collected and the eggs were counted. The Ovitrap index in three villages were observed at percentage more than 50%, with the highest OI detected in Kranji Village (97.56%). Female Aedes sp were monitored to lay their eggs outdoor than indoor. The OI is not always relevant to predict the dengue cases but could inform the risk of DENV tranmission in certain area. The community should do efforts to minimize potential breeding sites, mainly unused container outdoor to reduce the mosquito density.

scholarly journals Simulation Model for Dynamics of Dengue with Innate and Humoral Immune Responses

2018 ◽  
Vol 2018 ◽  
pp. 1-18 ◽  
Author(s):  
S. D. Perera ◽  
S. S. N. Perera
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Asymptomatic Infection ◽  
Severe Dengue ◽  
Viral Kinetics ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Kinetics Of

Dengue virus is a mosquito borne Flavivirus and the most prevalent arbovirus in tropical and subtropical regions around the world. The incidence of dengue has increased drastically over the last few years at an alarming rate. The clinical manifestation of dengue ranges from asymptomatic infection to severe dengue. Even though the viral kinetics of dengue infection is lacking, innate immune response and humoral immune response are thought to play a major role in controlling the virus count. Here, we developed a computer simulation mathematical model including both innate and adaptive immune responses to study the within-host dynamics of dengue virus infection. A sensitivity analysis was carried out to identify key parameters that would contribute towards severe dengue. A detailed stability analysis was carried out to identify relevant range of parameters that contributes to different outcomes of the infection. This study provides a qualitative understanding of the biological factors that can explain the viral kinetics during a dengue infection.

scholarly journals Epidemiological and Clinical Features of Dengue Infection in Adults in the 2017 Outbreak in Vietnam

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Bui Vu Huy ◽  
Le Nguyen Minh Hoa ◽  
Dang Thi Thuy ◽  
Nguyen Van Kinh ◽  
Ta Thi Dieu Ngan ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Dengue Fever ◽  
Clinical Features ◽  
Dengue Infection ◽  
Clinical Manifestations ◽  
Warning Signs ◽  
Severe Dengue ◽  
Severe Bleeding ◽  
Cross Sectional ◽  
Number Of Patients

Purpose. The clinical features and laboratory results of dengue-infected adult patients admitted to the hospital during the 2017 outbreak were analyzed in this study. Method. This is a cross-sectional study. 2922 patients aged 18 years or more with dengue fever in National Hospital for Tropical Diseases (NHTD) in the North and Hospital for Tropical Disease (HTD) in the South of Vietnam were recruited in this study. Result. Patients were admitted in the hospital around the year and concentrated from August to December, in 53/63 (84.0%) provinces in Vietnam, and patients in all ages were affected. The number of patients with dengue fever was 1675 (57.3%), dengue with warning signs 914 (31.3%), and severe dengue 333 (11.4%), respectively. Among patients with severe dengue, severe plasma leakage and dengue shock account for 238 (8.1%), severe organ impairment 73 (2.5%), and severe bleeding 22 (0.75%). The rate of mortality was 0.8%, and the outcome of dengue patients is worse in the elderly and people with underlying diseases. Conclusion. The 2017 dengue outbreak occurred in a larger scale than in the previous years in terms of time, location, and number of patients. More elderly patients were infected by dengue in this outbreak, and this may contribute to the mortality rate. Clinical manifestations of dengue patients in Southern Vietnam are more typical than the northern, but the rate of severe dengue is not different. The mortality risk and underlying conditions associated with dengue-infected elderly patients are worthy of further investigations in the future.

scholarly journals Quantitative NS1 antigen and the severity of dengue virus infections

2015 ◽  
Vol 55 (2) ◽  
pp. 87
Author(s):  
Ni Made Adi Purnami ◽  
Mohammad Juffrie ◽  
Made Gde Dwi Lingga Utama
Keyword(s):  
Clinical Course ◽  
Nonstructural Protein ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
Cross Sectional Study ◽  
Severe Dengue ◽  
Virus Infections ◽  
Cross Sectional ◽  
Nonstructural Protein 1 ◽  
Spearman Test

Background Dengue infection is one of the main cause ofmorbidity and mortality in children in Indonesia. Since it is knownthat earlier treatment and supportive therapies can decreased casefatality rate from dengue hemorrhagic fever (DHF), identificationof children who have risks to develop to DHF must be quicklyidentified, mainly in areas of endemic.Objective To find a correlation between increased quantitativesecreted nonstructural protein-1 (sNS1) with clinical course ofsevere dengue infections.Methods This was a cross-sectional study conducted on childrenwith dengue infections in Tropical Infections Division of ChildHealth Department, Sanglah Hospital, Denpasar. Detection ofthe dengue antigen was made by examining sNS1 quantitativeimmuno-assay. Analysis correlation of Spearman test was used tolook the relationship between increased quantitative sNS1 withclinical course of severe dengue infections.Results There was a positive relationship between quantitativesNS1 and clinical course of severe dengue infections with a valueof r = 0.903, P=0.001. Increased sNS1 level had a positivecorrelation with more severe dengue infections.Conclusions Quantitative sNS1 titer has a strong positivecorrelation with clinical course of severe dengue infections.

scholarly journals Levels of molecular angiogenic and antiangiogenic in pregnant women with risk of preeclampsia

2019 ◽  
Vol 8 (10) ◽  
pp. 4034
Author(s):  
Diah Wulandari ◽  
Mohamad Sulchan ◽  
Syarief Thaufik Hidayat
Keyword(s):  
Risk Factor ◽  
Pregnant Women ◽  
Molecular Level ◽  
Elisa Method ◽  
Cross Sectional ◽  
Primary Health ◽  
The Mean ◽  
Cross Sectional Design ◽  
Plgf Ratio ◽  
Risk Of Preeclampsia

Background: Angiogenic and antiangiogenic imbalances play a major role in the pathogenesis of preeclampsia. Increased production of sFlt-1 by the placenta causes free circulating PIGF and VEGF concentrations to lower because it is bound by sFlt-1. Measuring levels of angiogenic and antiangiogenic proteins as biomarkers indicates placental dysfunction and distinguishes preeclampsia from other disorders. This study aims to analyze the levels of angiogenic and antiangiogenic molecules in pregnant women at risk for preeclampsia.Methods: The study with a cross-sectional design was carried out in 11-15 weeks gestational age whom had a risk of preeclampsia with 30 samples in primary health care starting April-August 2018. Blood serum was measured by molecular levels of VEGF, PlGF, sFlt-1, and sFlt-1/PlGF ratio using the ELISA method. Data analysis used Pearson product moment test.Results: The mean of VEGF levels are 15.5±21.6, PlGF 89.7±55.5, sFlt-1 11519.4±5126.0 and the ratio sFlt-1/PlGF 166.7±102.1. Correlation value of risk factors for preeclampsia with molecular levels of VEGF r= -0.05; p = 0.76, PlGF r= -0.21; p = 0.26, sFlt r= 0.01; p =0.99 and ratio sFlt-1/PlGF r = 0.10; p = 0.58.Conclusions: The higher the total score of preeclampsia risk factor, the lower the molecular level of VEGF and PlGF is. Moreover, the higher the total score of preeclampsia risk factor, the higher the molecular level sFlt-1 and the sFlt-1/PlGF ratio is. There are no significant correlation between total score of preeclampsia risk factor and levels of molecule VEGF, PlGF, sFlt-1 and sFlt-1/PlGF ratio.

Survey of Waiting Time for Examination at the Medical Examination Department 2 of the National Children’s Hospital in 2018

2018 ◽  
Vol 2 (5) ◽  
Author(s):  
Dang Thi Hong Khanh ◽  
Nguyen Thi Ut ◽  
Nguyen Thi Chien ◽  
Giang Thi Thanh Ha ◽  
Vu Thi Thuy Linh
Keyword(s):  
Median Time ◽  
Waiting Time ◽  
Blood Test ◽  
Medical Consultation ◽  
Medical Examination ◽  
Medical Visit ◽  
Cross Sectional ◽  
The Mean ◽  
Cross Sectional Design

Objectives: To measure the waiting time for medical visit at the Outpatient Department 2, NationalChildren’s Hospital in 2018.Study method: Cross sectional design combines quantitative study.Result:The study on waiting time for medical visit of2060 patients showed that: the mean follow- up time was 32.5minutes. Average time for 01 technique was 137 minutes. The median time for medical consultation and 2techniques implemented was 162 minutes. The median time for the medical consultation and 03 techniquesimplemented was 208 minutes. Among sub- clinical techniques, blood test took the maximum time (137minutes).Conclusion: The waiting time for medical consultation was standardized according to the Decision 1313 /QĐ-BYT in 2013; Approximately 70 patients per visit / 8 hours.

scholarly journals Safe Blood Transfusion Practices among Nurses in a Major Referral Center in Ghana

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Agnes Asare Bediako ◽  
Rasheed Ofosu-Poku ◽  
Andrews Adjei Druye
Keyword(s):  
Blood Transfusion ◽  
Blood Products ◽  
Current Standard ◽  
Training Experience ◽  
Cross Sectional ◽  
Knowledge Questionnaire ◽  
The Mean ◽  
Cross Sectional Design ◽  
Safe Blood ◽  
Structured Questionnaire

Errors in transfusion of blood and blood products can lead to preventable morbidity and mortality. Nurses constitute a significant aspect of the transfusion process as they are the last in the chain of getting blood directly to the patient. They must, therefore, be conversant with the current standard of national and international guidelines on blood transfusion and appropriate management of adverse transfusion events. This study assesses the knowledge and practices of blood transfusion safety among nurses at Komfo Anokye Teaching Hospital. A descriptive cross-sectional design was employed, and structured questionnaire (Routine Blood Transfusion Knowledge Questionnaire) was used to collect data from 279 nurses from seven clinical directorates of the hospital. The data were processed with Stata version 14.0. Variables were analyzed using descriptive statistics, and relationships were drawn using inferential statistics. Over 90% of the respondents had a minimum of a diploma in nursing or midwifery, 63% had performed blood transfusion at least 5 times, and 46% had never received any training on blood transfusion. The mean score obtained in all four categories of blood transfusion knowledge assessed was 29, with 54% of the respondents scoring below the mean. The highest overall score on knowledge was 53%. This indicates that nurses had poor knowledge regarding safe blood transfusion practices as stipulated in the clinical guidelines for blood transfusion by Ghana’s National Blood Service. There was no statistically significant relationship between training/experience and knowledge of safe blood transfusion practices. Regular and continuous update training and audit are needed to safeguard patient safety during blood transfusion.

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