Cryotherapy Has No Significant Effect on MMP-9 anTGF-β1 Expression in Fungal Corneal Ulcer

BACKGROUND: Usually, surgical intervention is needed to eradicate the fungal microorganism that cause fungal corneal ulcers. However, since surgical intervention is invasive, the latest technology uses cryotherapy in treating it. Cryotherapy plays a vital role in the wound healing process. We aimed to evaluate is to study the decreased expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor β1 (TGF-β1) in fungal corneal ulcers after the administration of cryotherapy.METHODS: Aspergillus flavus fungus was injected to the intrastromal corneas of all Sprague Dawley rats. The rats were divided into four groups, the first group was not given any therapy, the second group was given topical natamycin therapy, the third group was given cryotherapy, and the fourth group was given a combination between cryotherapy and topical natamycin therapy. Therapy was given after five days of follow up on the formation of a corneal ulcer. After four days of therapy, the eyes were enucleated to determine MMP-9 and TGF-β1 expression.RESULTS: The result in the third group showed lower MMP-9 expression (20.0±10.0% cells per field of view) compared to the second group (40.0±20.0% cells per field of view) and the fourth group (30.0±25.0% cells per field of view), but had the same MMP-9 expression value as the first group. There was no significant difference in MMP-9 expression between the four groups (p=0.356). The third group reduced more TGF-β1 expression (10.0±12.50% cells per field of view) compared to the fourth group (30±27.5% cells per field of view) and the first group (30±32.5% cells per field of view). There was also no significant difference in TGF-β1 between the four groups (p=0.315).CONCLUSION: There is no significant difference in the expression of TGF-β1 and MMP-9 after the cryotherapy treatment.KEYWORDS: corneal ulcer, cryotherapy, MMP-9, TGF-β1

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Teratogenic effects of Metoprolol in the chick embryos

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v39i1.196 ◽

2015 ◽

Vol 39 (1) ◽

pp. 49-55

Author(s):

Abbas Razzaq Abed

Keyword(s):

Histopathological Examination ◽

Inflammatory Cells ◽

Heart Tissue ◽

Control Group ◽

Control Groups ◽

Teratogenic Effects ◽

Fourth Group ◽

The Third ◽

Significant Difference ◽

T1 And T2

This study was conducted to demonstrate the effect of Metoprolol on chicken embryos during the period of cuddling automated .This study was completed in Babylon hatchery dedicated to the production of chick chickens, which is located in the province of Babylon. One hundred eggs (Belgian origin, CZ 1924) were divided into equal four groups. The first group injected eggs by Metaprolol at dose 10 mg /70kg BW, and the second group eggs was injected by Metoprolol at dose 15 mg /70kg BW, while eggs in the third group was injected by physiological normal saline only and the fourth group did not inject their eggs any material and considered the control group and all the eggs used in this experiment to take on the fourth day of cuddling. The results of this study showed a decrease in the percentage hatching in groups injected with Metoprolol (T1 and T2 groups, 4% and 13%, respectively) compared to the T3 and the control groups. Also showed the results of the current study, no significant difference at the level of (P <0.05) in the weights of embryos after hatching. While histopathological examination showed the presence of pathological lesions in the heart tissue in injected eggs groups by Metoprolol (T1 and T2 groups) and included these changes infiltration of inflammatory cells, thickening in epicardium and the presence of vacuolation in heart tissue with the appearance of edema, heart tissue damage, congestion in the blood vessels and the occurrence of hemorrhage.

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Pengaruh Pengencer Tris Kuning Telur dan Andromed Terhadap Motilitas Spermatozoa Semen Sapi Friesian Holstein (Bos taurus) Sebelum dan Sesudah Pembekuan

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.10409 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Firlina Laila Putri ◽

Hari Santoso ◽

Husain Latconsina

Keyword(s):

Bos Taurus ◽

Block Design ◽

Egg Yolk ◽

Freezing Process ◽

Fourth Group ◽

Anova Test ◽

Spermatozoa Motility ◽

The Third ◽

Significant Difference ◽

Post Hoc

One of the most common problems in fluid-sperm’s dilution is a decrease in spermatozoa’s motility. The study aims to analyze the effect of tris-egg yolk and andromed-diluent to the spermatozoa motility of FH (Friesian Holstein) cattle’s fluid-sperm before and after the freezing. This research uses the experimental method with a randomized block design. It consist of two treatments; P1 = FH cattle’s fluid-sperm that was thinned using a tris-egg yolk and P2 = FH cattle’s fluid-sperm that was thinned an andromed-diluent. There are 4 sample group fluid-sperm from 4 cattle and it acted out 4 times. The obtained data were analyzed using ANOVA test and post hoc test. From ANOVA test, it showed that there are a significant difference (P<0.01) between tris-egg yolk and andromed-diluent in the reaction of spermatozoa’s motility value. The percentage of spermatozoa motility of fluid-sperm before freezing process is 55% for each group; however tris-egg yolk thinners are more able to maintain the motility or impulses spermatozoa of the Holstein Friesian fluid-sperm compared to andromed-diluent after freezing. The values of frozen spermatozoa’s motility with tris-egg yolk are 4.1±2.50 for first and fourth group and 42.5±2.89 for the second and the third group. While with andromed-diluent resulted 26.3±7.50 for the first group, 25.0±5.77 for the second group, 17.5±5.00 for the third group, and 18.8±7.50 for the fourth group. Keywords : Andromed, Bos taurus, spermatozoa’s motility, tris-egg yolk.ABSTRAKSalah satu masalah yang sering terjadi pada pengenceran semen yaitu penurunan motilitas spermatozoa. Penelitian ini bertujuan untuk menganalisa pengaruh pengencer tris kuning telur dengan pengencer andromed terhadap motilitas spermatozoa semen sapi FH (Friesian Holstein) sebelum dan sesudah pembekuan. Penelitian ini menggunakan metode eksperimen dengan Rancangan Acak Kelompok (RAK), terdiri dari 2 perlakuan yaitu P1 = semen sapi FH diencerkan menggunakan pengencer tris kuning telur dan P2 = semen sapi FH diencerkan menggunakan pengencer andromed, pada 4 kelompok sampel semen dari 4 ekor sapi dan dilakukan 4 kali ulangan. Data yang diperoleh dianalisis menggunakan Analisis Sidik Ragam (ANOVA) dan uji lanjut post hoc. Hasil analisis sidik ragam (anova) menunjukkan bahwa terdapat perbedaan yang sangat nyata (P<0,01) antara penggunaan pengencer tris kuning telur dengan pengencer andromed terhadap motilitas spermatozoa semen sapi FH. Hasil penelitian menunjukkan bahwa perlakuan pengencer tris kuning telur maupun andromed sama-sama dapat mempertahankan persentase motilitas spermatozoa semen sebelum pembekuan dengan persentase masing-masing kelompok yaitu 55%, namun pengencer tris kuning telur lebih mampu mempertahankan motilitas atau daya gerak dari spermatozoa semen sapi friesian holstein dibandingkan dengan pengencer andromed setelah pembekuan. Nilai motilitas spermatozoa semen beku pada pengencer tris kuning telur pada kelompok 1 dan 4 yaitu 41,3±2,50 dan pada kelompok 2 dan 3 sebesar 42,5±2,89 sedangkan pada pengencer andromed pada masing-masing kelompok yaitu 26,3±7,50; 25,0±5,77; 17,5±5,00 dan 18,8±7,50.Kata Kunci: andromed, Bos taurus, motilitas spermatozoa, tris kuning telur.

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Kelarutantumpatansementara Cavit dalamrendamansaliva buatan (Solubility of Cavit temporary filling immersed in artificial saliva)

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v13i2.391 ◽

2014 ◽

Vol 13 (2) ◽

pp. 69

Author(s):

Juni Jekti Nugroho ◽

Hasrul Husain

Keyword(s):

Artificial Saliva ◽

Distilled Water ◽

Fourth Group ◽

The Third ◽

Significant Difference ◽

Water Ph ◽

One Way Anova ◽

Temporary Filling

A continuous temporary filling inside the mouth would expose acid produced by microorganism during fermentation ofcarbohydrate that would lower the pH of saliva. The aim of this research is to determine the variation on solubilitylevels of Cavit temporary filling immersed in artificial saliva with pH 4, 6, and 8. Samples were placed in tubecontainers with 2 mm in height and 7 mm in diameter. Samples were immersed in four different groups of artificialsaliva based on the pH, in which the first group was immersed in artificial saliva pH 4, the second in pH 6, the third inpH 8, and the fourth group was immersed in distilled water (pH 7). The results showed that after 7 day of immersion,there was a significant difference on solubility levels between the four groups of treatment (p<0.05). The data weretabulated and analyzed using one-way ANOVA and least significant difference (LSD). Therefore, it can be concludedthat pH can affect the solubility levels of Cavit temporary filling.

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Effect Of Vitamine AND Selenum On Immune Response Of CO - Baghdad Vaccine In Sheep.

The Iraqi Journal of Veterinary Medicine ◽

10.30539/ijvm.v45i1.1066 ◽

2004 ◽

Vol 45 (1) ◽

pp. 55-66

Author(s):

A. M.H. AL-Judi ◽

R. H. Hameed ◽

A. D. Salman

Keyword(s):

Immune Response ◽

Body Weight ◽

Vitamin E ◽

Antibody Titer ◽

Control Group ◽

Fourth Group ◽

The Third ◽

Significant Difference ◽

And Control

This study was done on 57 Awassi pregnant ewes, divided in to 5 groups, the 1st control group, the second group received E (900 mg) and Selenium (3mg), twicel.M.injections of vitamin the third group received one injection of vitamin E (900mg) and feed via selenium ( 3 mg), the fourth group received vitamin E block (1 gm) daily for two weeks, the fifth group received vitamin E via feed block for one week (1gm daily), and all groups injected with C Baghdad vaccine. This study had been shown a significant difference in antibody titer between treated groups and the control group, from2weeks post titre vaccination There were significant difference in antibody (p<0.05) between lambs of treated and control group. While there were no significant difference in body weight of ewes and lambs, production of treated and but was a significant difference in milk control group.

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A Stepwise Approach to the Surgical Management of Hemorrhagic Choroidal Detachments

Journal of VitreoRetinal Diseases ◽

10.1177/2474126421992024 ◽

2021 ◽

pp. 247412642199202

Author(s):

Shanna C. Yeung ◽

Ryan H. Mason ◽

Samuel A. Minaker ◽

Alaa AlAli ◽

Peter J. Kertes ◽

...

Keyword(s):

Anterior Chamber ◽

Surgical Approach ◽

Corneal Ulcer ◽

Surgical Intervention ◽

Insertion Site ◽

Second Step ◽

Choroidal Detachment ◽

Stepwise Approach ◽

Trocar Insertion ◽

The Third

Purpose: This work describes a stepwise surgical approach to draining choroidal detachments and 2 cases for which this approach was used. Methods: The first step involves insertion of an anterior chamber maintainer and a nonvalved 23- or 25-gauge trocar cannula at the highest peak of hemorrhagic choroidal detachment (as determined using B-scan ultrasonography), 6 to 8 mm from and angled 20° to 30° toward the limbus. The second step involves removal of the trocar to expose the sclerotomy. Alternatively, the second step can be insertion of a second trocar. The third step involves the creation of a small focal peritomy around the preexisting sclerotomy and enlargement of the preexisting sclerotomy into a radial sclerotomy. Progression between steps only occurs if prior steps did not provide adequate drainage. Results: Two cases of appositional hemorrhagic choroidal detachments in hypotonic eyes were successfully resolved by this stepwise approach. In case 1, a choroidal detachment developed after a corneal ulcer perforation. The hemorrhagic choroidal detachment in case 1 was resolved with steps 1 and 2, and an unnecessary scleral cutdown was avoided. In case 2, a choroidal detachment developed after a trabeculectomy. The detachment in case 2 required progression to step 3, extension of the trocar insertion site into a radial sclerotomy. Conclusions: This stepwise approach should be considered to reduce excessive manipulation of the globe and conjunctiva in hemorrhagic and serous choroidal detachments that warrant surgical intervention.

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Hubungan antara Menopouse dengan Proses Penyembuhan Luka Paska Pencabutan Gigi di Klinik Gigi Universitas Wijaya Kusuma Surabaya

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v5i1.5 ◽

2018 ◽

Vol 5 (1) ◽

pp. 49

Author(s):

Wahyuni Dyah Parmasari ◽

Theodora Theodora ◽

Enny Willianti

Keyword(s):

Wound Healing ◽

Oral Health ◽

Tooth Extraction ◽

Healing Process ◽

Wound Healing Process ◽

Delayed Wound Healing ◽

The Third ◽

Significant Difference ◽

Main Factors ◽

Experimental Use

Backgrounds : In medicine faculty specially in oral health university of Wijaya Kusuma Surabaya, extracting is the most common cases in clinic. As we know geriatry patients needs this treats too. The newest reports explain that estrogenes affected production of collaagens. Collagens are needed for asselarate wound healing. Menopause process decrease production of estrogen, so it can be influance for collagens output product.Purpose : This experimental to discribe the connection between menopause with clinical wound healing process after tooth extraction at the Poli Gigi Universitas Wijaya Kusuma Surabaya. Methodes : This experimental use wound healing scale use parameters Tumor (swelling), Rubor (hyperemia) and Dolor (pain) Results : In this study used 20 patients divided two groups, menopause and non menopause group. There was a significant difference between menopause group and non menopause group in the third day and the fifth day after tooth extracted. For geriatry patients need dental care, specially tooth extraction. The complication in this age is delayed wound healing. For woman cases has many degeneration problems, one of this is because oldery ages woman has fisiology prosess menopause periode. Menopause is a regeneration process makes ovarium stops producting ovum. It was effected for maturation ovum and distrubing production of collagens. Collagens have main factors for wound healing process. In this articles, writer tried to connected the relations delayed wound healing in woman that have been in menopause period in case extracted teeth. Conclusion : There is a significant different between wound healing menopause and non menopause. The menopause the process wound healing takes more longer than non menopause.

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Comparing Intravenous Dexamethasone, Pethidine, and Ketamine for Postoperative Shivering Prevention in Patients Undergoing General Anesthesia

Quarterly of Horizon of Medical Sciences ◽

10.32598/hms.27.3.1807.2 ◽

2021 ◽

Vol 27 (3) ◽

pp. 288-301

Author(s):

Mohsen Saheban Maleki ◽

◽

Sepide Sedaghati Ansari ◽

Fateme Rezaniazave ◽

Alireza Talai ◽

...

Keyword(s):

General Anesthesia ◽

Orthopedic Surgery ◽

Controlled Study ◽

Control Group ◽

Double Blind ◽

Fourth Group ◽

The Third ◽

Dexamethasone Group ◽

Significant Difference ◽

Age Range

Aims: Postoperative shivering is unpleasant and annoying for the patient, occurring in up to 65% of cases undergoing general anesthesia. Various mechanisms were suggested for postoperative shivering. Shivering after anesthesia can cause complications, such as cardiovascular conditions, bleeding, and infection. This study compared the effects of dexamethasone, pethidine, and ketamine on the prevention of shivering after general anesthesia. Methods & Materials: In total, 164 patients with ASA classes one and two in the age range of 20-60 years under general anesthesia with orthopedic surgery, urology, and general surgery were included in the study. This study was a double-blind, randomized, placebo-controlled study. The study patients were divided into 4 groups of 41 subjects. After the induction of anesthesia and before surgery, in the first group, dexamethasone 0.15 mg/kg body weight, in the second group, ketamine 0.5 mg/kg, in the third group, pethidine 0.5 mg/kg, and in the fourth group, normal saline 0.9% were all given in 2 cc volume. After surgery, the examined patients were monitored for visible shivering by the researcher. The obtained data were analyzed using SPSS. Findings: The frequency and severity of shivering were lower in the dexamethasone (P=0.009), pethidine (P=0.004), and the ketamine (P=0.000) groups, compared to the control group. Besides, there was a significant difference between each of these 3 groups and the controls. The frequency and severity of shivering in the dexamethasone group were not significantly different from those of the pethidine group (P=0.565). The frequency and severity of shivering in the dexamethasone and ketamine groups were not statistically significant (P=0.071). The frequency and severity of shivering in the pethidine group with ketamine were not statistically significant (P=0.063). Conclusion: The obtained results indicated that dexamethasone, pethidine, and ketamine were effective in preventing postoperative shivering. There was no difference between these medications in the prevention of postoperative shivering.

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POSSIBILITY OF NEW LYMPHATIC PATHWAY CREATION THROUGH NEO-LYMPHANGIOGENESIS INDUCED BY SUBDERMAL DISSECTION

Lymphology ◽

10.2458/lymph.4789 ◽

2022 ◽

Vol 54 (3) ◽

Author(s):

T. Yamamoto ◽

N. Yamamoto

Keyword(s):

Quality Of Life ◽

Medical Records ◽

Surgical Intervention ◽

Healing Process ◽

Wound Healing Process ◽

Life Score ◽

Significant Difference ◽

Lymphatic Pathway ◽

Lymphatic Pathways

Surgical intervention and subsequent wound healing process are known to induce neo-lymphangiogenesis, but few studies have been reported to utilize this mechanism for lymphedema treatment. The aim of this study was to evaluate feasibility of subdermal dissection for neo-lymphangiogenesis induction (SDN) to treat lower extremity lymphedema (LEL). Medical records of secondary LEL patients who had undergone ICG lymphography and SDN procedure were reviewed. SDN was performed by dissecting fat tissues just below the dermis from the most proximal area showing dermal backflow through abdominal-toaxillary lymphatic pathways. Perioperative lymphedematous conditions were evaluated with lymphedema quality of life score (LeQOLiS) and LEL index. Seventeen female patients were included. SDN could be performed in 10 minutes on average without postoperative complication. Postoperative ICG lymphography showed new lymphatic pathways in 6 (35.3%) cases. Postoperative LeQOLiS ranged from 9 to 66, which was statistically lower than preoperative LeQOLiS (32.9 ± 19.2 vs. 36.6 ± 19.3, p = 0.048), whereas there was no statistically significant difference between pre- and post-operative LEL index (275.2 ± 23.3 vs. 270.5 ± 20.8, P = 0.073). Subdermal dissection, although its probability is not high, has a potential to induce neo-lymphangiogenesis. Further studies are required to improve and demonstrate efficacy of the procedure for new lymphatic pathway creation.

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The effect of ethanol extract of myrmecodia pendens to tgf-β1 expression and osteoblast cells after tooth extraction (the experimental research on cavia cobaya)

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v2i3.608 ◽

2017 ◽

Vol 2 (3) ◽

pp. 150

Author(s):

Efa Ismardianita ◽

Ellyza Nasrul ◽

Yanwirasti Yanwirasti ◽

Mieke Hemiawati

Keyword(s):

Group Treatment ◽

Tooth Extraction ◽

Healing Process ◽

Ethanol Extract ◽

Control Group ◽

Wound Healing Process ◽

Osteoblast Cell ◽

Osteoblast Cells ◽

Significant Difference ◽

Tgf Β1

Objective: Myrmecodia pendens is often used as a traditional medicine to treat various disease. A previous study, ethanol extract of sarang semut plant (Hypnophytum furmicarum jack) 4.65 mg was effective in fastening establishment of socket granulation tissue after tooth extraction, the Myrmecodia pendens 3% too fasten healing soft tissue of wound after tooth extraction. The aim of this study to determine the effect of ethanol extract of Myrmecodia pendens to the expression of TGF-β1, and the number of osteoblasts after tooth extraction.Material and Methods: this study using 24 male marmoots were divided into 2 groups. The entire groups had extract done 1st left incisor mandibula, into the socket control group applied 0,1 ml of 0.5% CMC and treatment group was given 0,1 ml of ethanol extract Myrmecodia pendens 10% dissolved with CMC 0.5%. On day 3,7,14 and 21, marmoots were decapitated and histopathologic preparations then observed the expression of TGF-β1, and the number of osteoblast cell. Data analyzed by Kruskall-wallis test with probability p<0.05 and Mann-Whitney to determine differences in effectiveness between variables.Results: Based on the Kruskal-wallis test, there is a significant difference the TGF-β1 expression and the number of osteoblast cell which had giving ethanol extract of Myrmecodia pendens between of control group and group treatment (p<0.05), further test with Mann-Whitney for TGF-β1 with days of observation shown a significant difference meanwhile osteoblast with days of observation showed no significant difference.Conclusion: the ethanol extract Myrmecodia pendans can assist the wound healing process after tooth extraction by increasing the expression of TGF-β1, and osteoblast cells number

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IgG4-related intracranial disease

The Neuroradiology Journal ◽

10.1177/1971400918806323 ◽

2018 ◽

Vol 32 (1) ◽

pp. 29-35 ◽

Cited By ~ 6

Author(s):

Shahine Goulam-Houssein ◽

Jeffrey L Grenville ◽

Katerina Mastrocostas ◽

David G Munoz ◽

Amy Lin ◽

...

Keyword(s):

Plasma Cells ◽

Surgical Intervention ◽

Case Series ◽

Hypertrophic Pachymeningitis ◽

Diagnostic Challenge ◽

Perineural Spread ◽

Related Disease ◽

The Third ◽

Igg4 Related Disease ◽

Intracranial Involvement

IgG4-related disease (IgG4-RD) is a multi-organ chronic inflammatory process caused by infiltration of IgG4-positive plasma cells in one or more organs. Intracranial involvement has only recently become better recognized. Our case series adds to the growing literature on the varying presentations of intracranial IgG4 by describing the clinical and imaging findings of three patients who presented to our institution with intracranial involvement. Our first patient presented with a mass-forming IgG4 pachymeningitis mimicking a sphenoid wing meningioma, which is to our knowledge the largest mass-forming pachymeningitis published in the literature. Our second case depicts another presentation of extensive IgG4 pachymeningitis involving both cavernous sinuses and surrounding Meckel’s caves. The third case describes a patient with presumed lymphocytic hypophysitis, which was later determined to be IgG4-related hypophysitis with concomitant pachymeningitis and perineural spread along the optic nerves. The delayed diagnoses in our cases illustrates the diagnostic challenge that clinicians face in differentiating intracranial IgG4-RD from other infiltrative diseases such as sarcoidosis, granulomatous disease, tuberculosis and lymphoma. Earlier consideration of IgG4-related hypophysitis and hypertrophic pachymeningitis in the differential diagnosis can prevent significant morbidity including unnecessary surgical intervention and organ failure secondary to extensive fibrosis.

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