Probiotic activity of Bifidobacteria from infant faeces

2016 ◽  
Author(s):  
Rita Narayanan ◽  
B. Suresh Subramonian
Keyword(s):  
Antimicrobial Activity ◽  
Probiotic Properties ◽  
Molecular Tools ◽  
Accession Number ◽  
Phenotypic Characteristics ◽  
Fermentation Profile ◽  
Carbohydrate Fermentation ◽  
Species Specific ◽  
Breast Fed ◽  
Probiotic Activity

In the present study four isolates belonging to three bifidobacterial species with probiotic properties were isolated and identified from the faeces of breast fed infants and incorporated in the preparation of a nutraceutical whey based malt food. Identification of isolates to the genus Bifidobacteria was based on phenotypic characteristics like the unique pleomorphic morphology and carbohydrate fermentation profile. Molecular tools like 16S rRNA targeted genus and species specific mPCR were used for confirmation. The four isolates (of 3 species) identified wereB. longum(IB10 and IB12)B. breve (IB39)and B.bifidum (IB42). The nucleotide sequences of the identified species were submitted to the GenBank for acquisition of accession number. The identified species were screened for probiotic properties like bile tolerance, antimicrobial activity and tolerance to acidity. All the four isolates showed more than 80 per cent viability in the presence of 0.4, 0.8 and 1 per cent of bile salt. IsolateB.longum (IB10)showed maximum antimicrobial activity against Bacillus cereus (zi3.03 ± 0.019 mm), Staphylococcus aureus (zi3.00 ± 0.031mm), Escherichia coli(zi2.98 ± 0.043 mm) and Pseudomonas aeruginosa (zi3.03 ± 0.051 mm) in the cell free supernatant. All the four bifidobacterial isolatesshowed more than 80 per centviability atpH 3.B.longum (IB10)was a better probiotic when compared to other isolates as it had maximum antimicrobial activity and tolerance to bile and acidity.

Genetic and phenotypic diversity of Bifidobacterium thermacidophilum fecal isolates from newborns

2007 ◽  
Vol 53 (12) ◽  
pp. 1348-1359 ◽  
Author(s):  
E. Kheadr ◽  
N. Dabour ◽  
U. von Ah ◽  
C. Lacroix ◽  
L. Meile ◽  
...  
Keyword(s):  
Phenotypic Diversity ◽  
Newborn Infants ◽  
Maximal Growth ◽  
Restriction Profile ◽  
Chromosomal Dna ◽  
Phenotypic Characteristics ◽  
Fermentation Profile ◽  
Carbohydrate Fermentation ◽  
Breast Fed ◽  
Distinct Restriction

This study was undertaken to genetically identify and phenotypically characterize 14 bifidobacteria isolated from 20 breast-fed newborns. These isolates showed 98%–99% similarity to Bifidobacterium thermacidophilum subsp. suis based on 16S rDNA. Further analysis by pulsed-field gel electrophoresis of chromosomal DNA digested with XbaI revealed 4 distinct restriction patterns. The predominant pattern, shared by 8 (57%) isolates, produced a macro-restriction profile with about 13 large fragments ranging in size from >242.5 to 23.1 kb, whereas the other 6 displayed 3 distinct restriction profiles all characterized by more micro- than macro-restriction, with fragments ranging in size from 97 to 9.4 kb. Phenotypic characteristics, including carbohydrate fermentation profile, maximal growth temperature, and antibiotic susceptibility, varied widely even among strains showing the same restriction profile. The presence of B. thermacidophilum in stools of newborn infants may indicate the potential of these bacteria for aiding the development of the intestinal ecosystem.

​Probiotics for Augmenting Health

2021 ◽  
Author(s):  
Rita Narayanan ◽  
B. Suresh Subramonian
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Techniques ◽  
Control Group ◽  
Probiotic Properties ◽  
Gut Health ◽  
Weaned Piglets ◽  
Log Reduction ◽  
Positive Effects ◽  
Breast Fed ◽  
Spray Dried

Background: The probiotic microorganism consists mostly of the strains of the genera Lactobacillus and Bifidobacteria. Bifidobacterial species are common members of the infant gut where they form up to 91 per cent of the total micro flora in breast-fed babies and up to 75 per cent in formula fed infants Methods: Bifidobacterial species were isolated and identified from the faeces of breast fed infants by molecular techniques. Molecular tools like 16S rDNA targeted genus and species specific mPCR primers were used for confirmation. A total of 4 isolates were identified namely B. longum (IB10 and IB12) B. breve (IB39) and B. bifidum (IB42). The identified species were screened for probiotic properties like bile tolerance, antimicrobial activity and tolerance to acidity. Ideal isolate of B. longum was incorporated in the preparation of whey based malted nutraceutical spray dried food and fed to weaned piglets. Result: B. longum (IB10) was the ideal probiotic when compared to other isolates as it had maximum antimicrobial activity and tolerance to bile and acidity. It was noted that isolate IB10 (B. longum) had an inhibitory zone ranging from 3.03 to 2.95 mm against B. cereus, S. aureus, E. coli and P. aeruginosa due to acid and antibacterial compounds. Isolate B. longum (IB10) was incorporated in the preparation of whey based malted nutraceutical spray dried food. The influence of feeding five grams of the spray dried nutraceutical malt food containing B. longum at 107cfu/g per day on animal production, gut health and gut architecture in young weaned piglets were also investigated. The probiotic regime had positive effects on the health of piglets when compared to the control group. A significant two log reduction in the coliform count was observed in the faeces of weaned piglets fed with Bifidobacterial nutraceutical powder than that of control. Thus, these probiotic bifidobacteria can be considered as safe adjuncts for preparation of dairy nutraceuticals.

scholarly journals Potential natural enemies of the invasive rugose spiraling whitefly, Aleurodicus rugioperculatus Martin in India

2017 ◽  
Vol 30 (4) ◽  
pp. 236 ◽  
Author(s):  
K. Selvaraj ◽  
R. Sundararaj ◽  
T. Venkatesan ◽  
Chandish R. Ballal ◽  
S. K. Jalali ◽  
...  
Keyword(s):  
Natural Enemies ◽  
Tamil Nadu ◽  
Ornamental Plants ◽  
Coi Gene ◽  
Molecular Diagnostic ◽  
Pest Species ◽  
Molecular Tools ◽  
Accession Number ◽  
Custard Apple ◽  
First Time

A invasive rugose spiraling whitefly (RSW) <em>Aleurodicus rugioperculatus</em> Martin (Hemiptera: Aleyrodidae) was found infesting coconut, banana, custard apple and several ornamental plants in Tamil Nadu, Andhra Pradesh and Kerala for the first time in India. The identity of the pest species was determined through morphological and molecular tools. Furthermore cytochrome c oxidase-I gene (658 bp) of RSW was sequenced (GenBank accession number KY209909) which would serve as an ideal molecular diagnostic marker for its identification irrespective of its phenotypic plasticity. During the survey, several natural enemies were recorded and maximum parasitism was recorded by <em>Encarsia guadeloupae</em> Viggiani (Hymenoptera: Aphelinidae) and its COI gene was sequenced and deposited as <em>Encarsia</em> sp. (GenBank accession number KY223606). Per cent parasitism ranged from 20.0 to 60.0 % in different collection locations, highest parasitism being recorded in Kerala as compared to other states. The predators recorded were <em>Mallada</em> sp., few coccinellids and predatory mites. This communication is the first report of the rugose spiraling whitefly, its host plant range and associated natural enemies in India.

scholarly journals Uneven distribution of cryptic diversity among higher taxa of parasitic worms

2010 ◽  
Vol 7 (2) ◽  
pp. 241-244 ◽  
Author(s):  
Robert Poulin
Keyword(s):  
Cryptic Species ◽  
Zoonotic Diseases ◽  
Helminth Parasites ◽  
Morphological Markers ◽  
Molecular Tools ◽  
Invasive Pathogens ◽  
Higher Taxa ◽  
As Species ◽  
Species Specific ◽  
Parasitic Worms

Cryptic species cause problems for estimates of biodiversity. In the case of parasites, cryptic species also plague efforts to detect potential zoonotic diseases or invasive pathogens. It is crucial to determine whether the likelihood of finding cryptic species differs among higher parasite taxa, to better calibrate estimates of diversity and monitor diseases. Using published reports of cryptic species of helminth parasites identified using molecular tools, I show that the number of species found is strongly related to the number of parasite individuals sequenced, weakly influenced by the number of host species from which parasites were obtained, and unaffected by the genetic markers used. After correction for these factors, more cryptic species of trematodes are found than in other helminth taxa. Although several features distinguish trematodes from other helminths, it is probable that our inability to discriminate among sibling species of trematodes results from their lack of structures serving as species-specific morphological markers. The available data suggest that current estimates of helminth diversity may need to be doubled (tripled for trematodes) to better reflect extant diversity.

scholarly journals Microsatellite loci development and population genetics in Neotropical fish Curimata mivartii (Characiformes: Curimatidae)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5959 ◽  
Author(s):  
Ricardo M. Landínez-García ◽  
Edna J. Marquez
Keyword(s):  
Population Genetics ◽  
Microsatellite Loci ◽  
Molecular Tools ◽  
Migratory Species ◽  
Lower Section ◽  
Neotropical Fish ◽  
International Union ◽  
Commercially Important ◽  
Species Specific ◽  
Near Threatened

The Curimatidae family plays an ecological role in the recycling and distribution of nutrients and constitutes a major food source for several commercially important fishes. Curimata mivartii, a member of this family, is considered a short-distance migratory species (≤100 km), categorized by the International Union for Conservation of Nature as a near threatened species, based on its declining population densities and habitat disturbance and fragmentation. Since population genetics and species-specific molecular tools remain unknown for all members of the Curimatidae family, this study developed a set of microsatellite loci and studied the population genetics of C. mivartii in the lower section of the Colombian Magdalena-Cauca basin. The results showed high levels of genetic diversity and evidence of gene flow even between locations separated over 350 km. This information provides a baseline for designing conservation and management programs for C.mivartii and constitutes the first study of population genetics in Curimatidae.

Identification and Evaluation of Probiotic Potential in Yeast Strains Found in Kefir Drink Samples from Malaysia

2019 ◽  
Vol 15 (7) ◽  
Author(s):  
Mohd Akmal Azhar ◽  
Mimi Sakinah Abdul Munaim
Keyword(s):  
Antimicrobial Activity ◽  
Probiotic Properties ◽  
E Coli ◽  
Probiotic Potential ◽  
Rdna Sequences ◽  
Yeast Strains ◽  
16S Rdna Sequences ◽  
Human Body Temperature ◽  
Probiotic Yeast ◽  
New Yeast

AbstractKefir drink is a source of probiotic microorganism with remarkable functional and technological properties. The objective of this work is to isolate yeast strains from Malaysian kefir drink and evaluate them for probiotic potentials. In the present study, nine strains of probiotic yeast were isolated from a Malaysian kefir drink and identified according to their 16S rDNA sequences. Furthermore, their probiotic potential was evaluated. The probiotic properties were tested for aspects of antibiotic susceptibility, antimicrobial activity, and gastrointestinal condition tolerance (pH and temperature). Five isolated strains, M3, Y5, Y9, Y11 and A1, showed good tolerance towards low pH condition while three strains, A1, M1, and M3, showed antimicrobial activity against E. coli, P. aeruginosa, and Salmonella sp. Most isolates were resistant to penicillin, streptomycin, and ampicillin, and grew well at human body temperature. The result of this test indicates that the yeast strains isolated from Malaysian kefir drink have excellent potential for use as probiotics in various products. Lastly, kefir milk is one of the excellent source of probiotic yeast strains and could be used as a new yeast probiotic formulation or in food supplements.

Antimicrobial activity against Shigella sonnei and probiotic properties of wild lactobacilli from fermented food

2011 ◽  
Vol 167 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Yingchun Zhang ◽  
Lanwei Zhang ◽  
Ming Du ◽  
Huaxi Yi ◽  
Chunfeng Guo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fermented Food ◽  
Shigella Sonnei ◽  
Probiotic Properties

scholarly journals Resequencing the Genome of Bifidobacterium breve Strain CECT7263

2017 ◽  
Vol 5 (18) ◽  
Author(s):  
Noelia Martínez ◽  
Roberto Luque ◽  
Mónica M. Olivares ◽  
Abelardo Margolles ◽  
Oscar Bañuelos
Keyword(s):  
Genomic Data ◽  
Probiotic Properties ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Bifidobacterium Breve

ABSTRACT The probiotic properties of Bifidobacterium breve CECT7263, as well as its safety, have been the focus of in several studies since 2008, including the sequencing of its genome in 2012. This study aims to complete the available genomic data to deepen the knowledge of some phenotypic characteristics of this strain.

scholarly journals PROBIOTIC CHARACTERIZATION OF BACILLUS SUBTILIS STRAIN ISOLATED FROM INFANT FECAL MATTER REVEALED BY 16S rRNA GENE AND PHYLOGENETIC ANALYSIS

2021 ◽  
pp. 77-85
Author(s):  
DEVARANJAN DAS ◽  
CHANDI CHARAN RATH ◽  
NAKULANANDA MOHANTY ◽  
SMITA HASINI PANDA
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Bile Salt ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Fecal Matter ◽  
Antimicrobial Metabolites

Objective: The rationale of our study was to isolate and identify the putative probiotic strain from infant fecal matter exhibiting a broad range of antimicrobial activity and to analyze the effect of different culturing conditions on its probiotic properties and the production of antimicrobial metabolites. Methods: In the present study, bacterial strains were screened for probiotic properties and antimicrobial activity from infant fecal matter (6 months–2 years). The effect of varying culture conditions such as tolerance to acid, bile salt, phenol, NaCl, pH, incubation period, and temperature along with autoaggregation assay, hydrophobicity, and hemolysis was studied. The characterization of the potent strain was studied by morphological, biochemical, and 16S rRNA gene sequencing along the phylogenetic affiliation of the strain was studied. Results: Two putative probiotic bacteria (DAM and IFM) were isolated, identified, characterized, and predicted at pH 2.0, 3.0, and 4.0, the isolate IFM had 50%, 60%, and 70% survivability, while isolate DAM had 55%, 63%, and 75% survivability, respectively. At a bile salt concentration of 0.5%, both isolates had a 75% survival rate. The isolates exhibited a high percentage of hydrophobicity and autoaggregation. The isolates also had non-hemolytic activity and were susceptible to many clinical tested antibiotics (tetracycline, erythromycin, ampicillin, gentamycin, penicillin, etc.). The isolate showed antimicrobial activity against enteric pathogens such as Staphylococcus aureus, Escherichia coli, and Shigella dysenteriae. The accession number of Bacillus subtilis MT279753 and MK453362 was submitted to NCBI. Conclusion: The result revealed that isolates have potent probiotic properties and possess a direct influence on the production of antimicrobial metabolites. These parameters can be modified for the improvement of the potentiality of the isolates.

scholarly journals Diversity of lactic acid bacteria in dadih produced by either back-slopping or spontaneous fermentation from two different regions of West Sumatra, Indonesia

2019 ◽  
Vol 12 (6) ◽  
pp. 823-829 ◽  
Author(s):  
Chandra Utami Wirawati ◽  
Mirnawati Bachrum Sudarwanto ◽  
Denny Widaya Lukman ◽  
Ietje Wientarsih ◽  
Eko Agus Srihanto
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Spontaneous Fermentation ◽  
Lactobacillus Pentosus ◽  
Gene Sequence Analysis ◽  
West Sumatra ◽  
Fermentation Profile ◽  
Carbohydrate Fermentation ◽  
Abundance And Diversity ◽  
Different Origins

Aim: Dadih samples from two different origins (Kamang and Gadut in West Sumatra) manufactured with different methods (back-slopping or spontaneous fermentation) were evaluated for the diversity of lactic acid bacteria (LAB). Materials and Methods: Four dadih samples manufactured with two different fermentation methods were obtained from Kamang and Gadut regions. Both genotypic and phenotypic characteristic (16S rRNA partial gene sequence analysis and carbohydrate fermentation profile) were used to analyze the diversity of dadih LAB population. Results: This study showed that LAB count in back-slopping fermented dadih was one log cycle higher than spontaneous fermented dadih. LAB isolates from the two regions were divided into three genera, namely Lactococcus, Lactobacillus, and Pediococcus. Sequencing results showed that 41.6% (five isolates) were identified as Lactococcus lactis ssp. lactis, 25% (three isolates) were identified as Lactobacillus plantarum ssp. plantarum, 16.6% (two isolates) were identified as L. lactis ssp. cremoris, and 8.3% (one isolate each) were identified as Pediococcus pentosaceus and Lactobacillus pentosus. Conclusion: Five species were determined in back-slopping fermented dadih, i.e., L. lactis ssp. lactis, L. lactis ssp. cremoris, L. plantarum ssp. plantarum, L. pentosus, and P. pentosaceus. On the other hand, spontaneous fermented dadih only contained three different species, namely L. lactis ssp. lactis, L. lactis ssp. cremoris, and L. plantarum ssp. plantarum. This research showed that back-slopping fermentation offers greater abundance and diversity compared to spontaneous fermentation in dadih.

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