Effect of graded concentration of organic Zinc (Zinc glycinate) on antioxidants status and immune response in commercial broilers

2015 ◽  
Author(s):  
K. Sridhar ◽  
D. Nagalakshmi ◽  
D. Srinivasa Rao ◽  
S. V. Rama Rao
Keyword(s):  
Immune Response ◽  
Antioxidant Status ◽  
Basal Diet ◽  
Cell Mediated Immunity ◽  
Broiler Chicks ◽  
Sod Activity ◽  
Organic Zinc ◽  
Antibody Titres ◽  
Peroxidase Enzyme ◽  
Organic Zn

One hundred and twenty day old commercial broiler chicks were randomly allotted to 4 dietary groups, with 6 replicates of 5 birds in each and reared for 42 days under uniform management conditions to study the effect of organic zinc (zinc glycinate; Zn-gly) supplemented at lower levels (30, 20 and 10 ppm) on antioxidant status and immune response in comparison to NRC (1994) recommended levels (40 ppm) of Zn supplemented from inorganic source (ZnSO4). The dietary treatments were corn soybean meal basal diet supplemented with 40 ppm Zn from ZnSO4 (inorganic) (control) and 30, 20 and 10 ppm Zn from Zn-gly (organic). Antioxidant enzyme levels and antibody titres against New castle disease (ND) vaccine (humoral immunity) were estimated on 35th d and oxidative stress markers from liver on 42nd d. On 40th d, the cell mediated immunity (CMI) was assessed as cutaneous basophilic hypertrophy to phytohemagglutinin-P (PHA-P). The lipid peroxidation in haemolysate (P<0.05) lowered in birds with 30 ppm Zn supplemented as Zn-gly compared to 20 and 10 ppm Zn from Zn-gly and 40 ppm Zn from ZnSO4. The Glutathione peroxidase enzyme activity was higher (P<0.05) with 30 ppm Zn as Zn-gly compared to control. The CMI was sgnificantly (P<0.05) high at 30 ppm Zn supplemented as Zn-gly compared to 40 ppm Zn as ZnSO4 and other organic Zn groups. The SOD activity, humoral and cell mediated immune resonse when fed 20 ppm Zn as Zn-gly was comparable to those on 40 ppm Zn as ZnSO4. The results of the study indicated that reducing the level of Zn from inorganic source (40 ppm) to 50 % (20 ppm) supplementation from Zn-gly can result in comparable immune and antioxidant status in broiler chicks.

Effect of replacing inorganic zinc with a lower level of organic zinc (zinc propionate) on performance, biochemical constituents, antioxidant, immune and mineral status in buffalo calves

2017 ◽  
Author(s):  
D. Nagalakshmi ◽  
K. Sridhar ◽  
M. Satyanarayana ◽  
S. Parashu Ramulu ◽  
V. S. Narwade ◽  
...  
Keyword(s):  
Immune Response ◽  
Antioxidant Status ◽  
Average Daily Gain ◽  
Serum Antibody ◽  
Antioxidant Enzyme Activities ◽  
Buffalo Calves ◽  
Biochemical Constituents ◽  
Organic Zinc ◽  
Antibody Titres ◽  
Organic Zn

An experiment of 120 days was carried out to investigate the effects of replacing inorganic zinc (ZnSO4; 80 ppm) with lower level (75% of inorganic levels; 60 ppm) of organic zinc (Zn propionate; Zn-Prop) on growth, serum biochemical constituents, immune response, mineral and antioxidant status in buffalo calves. Twelve buffalo calves (193.3±19.63kg; 14-24 months) were randomly allotted to a control (80 ppm Zn) and experimental (60 ppm Zn) diets. All calves were weighed at fortnight interval. Animals were immunized against Brucella abortus (BA) and chicken RBC (CRBC) antigens on day 90 with a booster dose of 15 days interval and serum was collected on 7, 14, 21 and 28 d of post sensitization (PS) to study the antibody titres. Blood was collected on 114 d of experiment to prepare serum and haemolysate. Cell mediated immune response (CMIR) was determined against phytohemagglutinin-P (PHA-P) on day 118. The fortnightly body weights, average daily gain, dry matter intake, feed conversion ratio were comparable between the dietary groups. Similarly, serum albumin, globulin, creatinine and mineral (Zn, Cu, Mn, Fe) levels were comparable while higher (P less than 0.05) serum total protein and alkaline phosphatase activities were observed in organic Zn supplemented calves. After 7 d of PS, serum antibody titres (log2) against BA were higher (P less than 0.05) with Zn-Prop supplementation, while the titres against CRBC were not affected by Zn source and dose. Lipid peroxidation and antioxidant enzyme activities (glutathione reductase, RBC catalase and superoxide dismutase) estimated in haemolysate were comparable between groups, while glutathione peroxidase (P less than 0.05) activity increased with Zn-prop supplementation compared to ZnSO4. The CMIR was higher (P less than 0.05) with organic Zn. It can be concluded that the growth performance, antioxidant status, and immune response in calves fed lower levels of organic Zn (Zn-Propionate) (75% of inorganic mineral supplementation) was comparable to those fed 100% of inorganic zinc and the CMI response was higher in organic Zn supplemented calves.

scholarly journals Evaluation of blood chemical, lipids profile and immune response on broiler chicks fed with milk thistle (Silybum mari- anum L.) and thyme (Thymus vulgaris L.) seeds in south-eastern Iran

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Hossein Ansari Nik ◽  
Omid Fani Makki ◽  
Ahmad Ebrahimzadeh ◽  
Arash Omidi
Keyword(s):  
Immune Response ◽  
Total Protein ◽  
Control Diet ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Lipoprotein Cholesterol ◽  
Thymus Vulgaris ◽  
Milk Thistle ◽  
Broiler Chicks ◽  
Lipids Profile

This study was conducted to investigate the effects of supplementation of milk thistle seeds (MTS) and thyme seeds (TS) on blood chemical, lipid profile and immune response in broiler chicks. In this study, 160 one-day-old chicks (Ross 308) were allocated to four treatments with four replicates based on a completely randomized design in a 2×2 factorial arrangement. The treatment groups were (A) basal control diet, (B) basal diet with 0.2 g/kg of MTS, (C) basal diet with 0.2 g/kg of TS and (D) basal diet with 0.2 g/kg of MTS and 0.2 g/kg of TS. Birds fed the (D) supplemented treatment (MTS plus TS) had the greatest levels of total protein, 4.26±0.27 g/L; albumin, 2.21±0.02 g/L; globulin 2.28±0.23 g/L; and aspartate aminotransferase (AST) activity, 152.18±4.46 U/L than the control birds [group (A)] (P&lt;0.05). MTS alone or in combination with TS reduced the cholesterol in the serum of the broilers (P&lt;0.05), and this effect was more pronounced for the (C) treatment (TS alone) (P&lt;0.05). Treatment consumption with MTS plus TS [group (D)] significantly increased the concentration of high-density lipoprotein cholesterol (HDL-C), 90.32±2.28 mmol/L, but low-density lipoprotein cholesterol (LDL-C), 10.44±0.07 mmol/L, and triglyceride concentrations, 60.75±2.65 mmol/L, were decreased compared to control (P&lt;0.05). However, none of the immunity parameters and liver enzymes differed significantly in MTS or TS groups. The present research indicated that supplements of MTS and TS have a protective influence on the lipids profile, total protein, globulin, albumin and AST levels in broiler chicks.

Immunogenicity of a DNA vaccine expressing the Neospora caninum surface protein NcSRS2 in mice

2009 ◽  
Vol 57 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Zhanzhong Zhao ◽  
Jun Ding ◽  
Qun Liu ◽  
Ming Wang ◽  
Jinshu Yu ◽  
...  
Keyword(s):  
Immune Response ◽  
Dna Vaccine ◽  
Neospora Caninum ◽  
Serum Antibody ◽  
Pcr Amplification ◽  
Surface Protein ◽  
Cell Mediated Immunity ◽  
E Coli ◽  
Mammalian Expression ◽  
Antibody Titres

The immunogenicity of a DNA vaccine expressing the surface protein NcSRS2 of Neospora caninum was studied in BALB/c mice. The NcSRS2-encoding DNA was obtained by PCR amplification of the NcSRS2 ORF gene from the p43 plasmid encoding the N. caninum surface protein NcSRS2, ligated to the mammalian expression vector pcDNA3.1/Zeo(+) and propagated in E. coli DH5α to produce the N. caninum NcSRS2 DNA vaccine. BALB/c mice were immunised by two intramuscular injections of the DNA vaccine with or without complete Freund’s adjuvant (CFA). Serum antibody titres and nitric oxide (NO) concentrations, and splenocyte proliferation and cytokine expression were measured after immunisation. The DNA vaccine induced T-cell-mediated immunity as shown by significantly increased NO concentrations, cytokine gene (IL-2 and IFN-γ) expression, and NcSRS2 protein-stimulated lymphocyte proliferation in mice immunised with the DNA vaccine. The vaccine also induced weak humoral immunity. The immunogenicity of the DNA vaccine was slightly enhanced by CFA. The immune response was specific to NcSRS2. No immune response was observed in mice immunised with the pcDNA3.1/Zeo(+) vector alone.

scholarly journals Effects of Ginger Extract on Laying Performance, Egg Quality, and Antioxidant Status of Laying Hens

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 857 ◽  
Author(s):  
Wen ◽  
Gu ◽  
Tao ◽  
Cheng ◽  
Wang ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Laying Hens ◽  
Egg Quality ◽  
Basal Diet ◽  
Cholesterol Content ◽  
Ginger Extract ◽  
Sod Activity ◽  
Egg Weight ◽  
Laying Performance ◽  
Yolk Cholesterol

The objective of this study was to investigate the effects of ginger extract (GE) as a dietary supplement for laying hens. A total of 40-week-old 288 Hyline Brown laying hens were randomly divided into two groups with six replicates, and fed a basal diet with or without 100 g/t GE for eight weeks. Dietary GE supplementation increased egg weight, albumin height, and Haugh unit of eggs, and decreased yolk cholesterol content and activities of alanine transaminase and aspartate transaminase in serum at eight weeks. Moreover, GE resulted in higher total superoxide dismutase (T-SOD) activity and lower malondialdehyde (MDA) content in yolk at four and eight weeks and in serum. It was concluded that GE was effective in increasing egg weight and improving the egg quality and antioxidant status of laying hens.

Effect of Potenmic on the immunity, blood metabolites and antioxidant status of broiler chicks

2020 ◽  
pp. 17-19
Keyword(s):  
Antibody Titre ◽  
Antioxidant Status ◽  
Crude Protein ◽  
Protein Concentration ◽  
Basal Diet ◽  
Serum Glucose ◽  
Blood Metabolites ◽  
Broiler Chicks ◽  
Fourth Group ◽  
Treatment Groups

The aim of the present study was to find the effect of Potenmic supplementation on humoral immunity, some blood metabolites and stress status in broiler. A total of 150 day old broiler birds were divided into five treatment groups. One group was control having crude protein and metabolizeble energy of 22% and 3000 Kcal/kg respectively as a basal diet. Second group was basal diet + 1ml Potenmic dissolved in 1.5 lit of water (NCPot1), third group was basal diet plus 1 ml Potenmic dissolved in 3 lit of water (NCPot2), fourth group was basal diet + 1 ml Potenmic dissolved in 5 lit (NCPot3) of water. Every group was divided into three replicates and each replicates was consisting of 10 birds. Antibody titre against ND was significantly (P<0.01) higher in NCPot1 and NCPot2 in comparison with the control. However, antibody titre against IB was significantly (P<0.05) higher in the treatment groups compared to the control. The H:L was significantly (P<0.01) lower in NCPot1 and NCPot2. Blood glucose was significantly (P<0.01) higher in the treatment groups compared to the control. However, NCPot1 and NCPot2 showed significantly (P<0.05) higher serum glucose and protein concentration compared to the control and other treatment groups. Blood MDA concentration was also significantly (P<0.05) lower in NCPot1 and NCPot2 compared to the control. The results of the present study showed that supplementation of Potenmic improved the immune response, blood metabolites and antioxidant status of broiler.

Prototype Pentavalent Bovine Leptospira Vaccines Blended with Oil Adjuvant Provides Protection and Prevents Renal Colonization in Guinea Pig Model

2021 ◽  
Author(s):  
K. Senthilkumar ◽  
R.P. Aravindhbabu ◽  
G. Ravikumar
Keyword(s):  
Immune Response ◽  
Aluminium Hydroxide ◽  
Guinea Pigs ◽  
Tamil Nadu ◽  
Cell Mediated Immunity ◽  
Produce Cell ◽  
Protective Antibody ◽  
Cell Mediated Immune Response ◽  
Antibody Titres ◽  
Bovine Leptospirosis

Background: Leptospirosis significantly impacts the economy of livestock farmers by causing reproductive failure and production losses in bovines. The control of bovine leptospirosis requires a combination of vaccination programme, biosecurity measures and chemotherapeutic regimens. Vaccination protect the clinical diseases and reduces/prevents renal colonisation of Leptospires and in turn reduces excretion of leptospires in urine which is a major source of environmental contamination. Some serovars of leptospires require cell mediated immunity apart from humoral immunity. This study was undertaken to develop prototype vaccine with prevalent serogroups to induce humoral and cell mediated immunity. Immunogenicity of the prototype vaccines and their safety were assessed experimentally in Guinea pigs.Methods: An inactivated oil adjuvant prototype pentavalent bovine leptospira vaccines with serogroups Australis, Hebdomadis, Hardjo, Javanica and Pomona which were prevalent in Tamil Nadu were developed and compared with the aluminium hydroxide gel adjuvant vaccine. The humoral immune response was assessed measuring antibodies by Microscopic Agglutination Test and Cell Mediated Immune response was assessed by lymphocyte proliferation assay. The ability of these vaccines to protect Guinea pigs against virulent Leptospires challenge was also demonstrated.Result: The prototype vaccine blended with oil adjuvants resulted in higher protective antibody titres of more than 6.64 log2 (≥1:100) than the aluminium hydroxide adjuvant blended vaccine. The protective antibody titres lasted upto 180 days, except for serogroup Javanica (150 days). The Montanide adjuvants were able to produce cell mediated immune response for protection against serovar Hardjo. Challenge study in guinea pigs showed complete protection following vaccination using pentavalent vaccine blended with Montanide adjuvants, while the aluminium hydroxide adjuvant was able to confer only partial protection. The Montanide blended prototype vaccines were also able to prevent renal colonisation of all five serogroups. This study shows the potential of the oil adjuvant blended multivalent vaccine for use in vaccination programmes against bovine leptospirosis.

scholarly journals Effect of a multivitamin complex and probiotic blend in drinking water before and after vaccination on performance traits, blood biochemistry and humoral immune response of broilers

2019 ◽  
Vol 44 (1) ◽  
pp. 28
Author(s):  
N. Jafarpour ◽  
F. Javandel ◽  
S. Gamboa ◽  
A. Seidavi ◽  
V. Tufarelli ◽  
...  
Keyword(s):  
Immune Response ◽  
Drinking Water ◽  
Humoral Immune Response ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Experimental Period ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Humoral Immune ◽  
Before And After

This study was conducted in order to investigate the effects of a multivitamin complex and probiotic blend in drinking water before and after vaccination on growth performance, carcass traits, blood biochemical parameters and humoral immune response of broiler chickens. A total of 150 one day-old male broiler chicks (Ross 308) were randomly allocated to five treatment groups, with three replicates (10 birds per replicate) in a completely randomized design. Experimental treatments consisted of: 1) a basal diet and drinking water without any additives as control (C); 2) C + drinking water + multivitamin complex from 2 days before vaccination until 3 days after vaccination; 3) C + drinking water + multivitamin complex for 3 days after vaccination; 4) C + drinking water + probiotic blend from 2 days before vaccination until 3 days after vaccination; and 5) C + drinking water + probiotic blend for 3 days after vaccination. In the whole experimental period, feed intake, body and thigh weight and abdominal fat were significantly affected (P<0.05) by dietary treatments. On the contrary, no significant effect was observed on carcass characteristics, feed conversion ratio, blood parameters, antibody production against SRBC and IgG. The titer of IgM was higher in treatment (2) than other treatments at 28 d of age (P<0.05). In conclusion, a multivitamin complex supplied in drinking water for 2 days before vaccination till 3 days after vaccination is enable to improve broiler performance and immunity.

scholarly journals Effects of organic zinc supplementation in weaned calves

2016 ◽  
Vol 85 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Sylva Dresler ◽  
Josef Illek ◽  
Ladislav Zeman
Keyword(s):  
Metabolic Profile ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Treated Group ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Sod Activity ◽  
Organic Zinc ◽  
Zinc Concentrations ◽  
Organic Zn

The aim of the study was to investigate the effect of organic zinc supplementation in calves on serum zinc (Zn) concentrations, selected metabolic profile indicators and serum immunoglobulin (Ig) concentrations. The trial included 2 groups (n = 10) of weaned female calves. The Zn-Methionin calves (group Zn-Met) were supplemented with 30 mg Zn-Met/kg dry matter (DM)/day (BIOPLEX® Zn, Alltech, USA) for 90 days; the control calves (group C) received the same diet without organic zinc supplementation. Compared to the control group, organic Zn treatment significantly increased serum Zn concentration (P < 0.05), superoxide dismutase (SOD) activity (P < 0.01) and total Ig (P < 0.01) in the group Zn-Met at the beginning (7 days from the start of Zn-Met supplementation) of the trial. At the end of the trial (day 90) serum total protein (TP) (P < 0.05), albumin (P < 0.01), urea (P < 0.01), SOD (P < 0.01), copper (Cu) (P < 0.05), Zn (P < 0.01) and Ig (P < 0.05) concentrations were significantly higher in the Zn-Met calves. In the control group alkaline phosphatase (ALP) activity was significantly (P < 0.01) higher on day 90. A positive correlation between zinc concentrations, ALP, and SOD activities in serum, and a negative correlation between zinc and copper concentrations were demonstrated. Dietary Zn-Met supplementation in calves markedly influenced the metabolic profile and serum immunoglobulin concentrations. Compared to the control group, the Zn supplemented group showed a significantly (P < 0.05) lower ALP and significantly greater SOD serum activity (P < 0.01) at the end of the trial. Total Ig concentrations were significantly higher in the Zn treated group (day 7: P < 0.01 vs. day 90: P < 0.05).

scholarly journals Organic zinc absorption by the intestine of broilersin vivo

2017 ◽  
Vol 117 (8) ◽  
pp. 1086-1094 ◽  
Author(s):  
Yu Yu ◽  
Lin Lu ◽  
Su-Fen Li ◽  
Li-Yang Zhang ◽  
Xu-Gang Luo
Keyword(s):  
Broiler Chickens ◽  
Basal Diet ◽  
Mrna Levels ◽  
Organic Zinc ◽  
Zn Addition ◽  
Zn Content ◽  
Acid Chelate ◽  
Diet Control ◽  
Hepatic Portal ◽  
Organic Zn

AbstractIn Expt 1, a Zn-unsupplemented basal diet (control) and the basal diet supplemented with one of four different Zn sources, including ZnSO4, Zn-amino acid chelate with a weak chelation strength (Zn-AA W), Zn-protein chelate with a moderate chelation strength (Zn-Pro M) and Zn-protein chelate with a strong chelation strength (Zn-Pro S) were fed to broiler chickens from days 14 to 28. On day 28, Zn content in plasma from the hepatic portal vein increased (P<0·05) in the following order: control<ZnSO4<Zn-AA W<Zn-Pro M<Zn-Pro S. Meanwhile, metallothionein (MT) mRNA levels in the duodenum, jejunum and ileum were enhanced (P<0·05) by Zn addition regardless of Zn source. However, no differences among different Zn sources were observed. In Expt 2, ligated duodenal loops from Zn-deficient broilers (28-d old) were perfused with solutions containing 0–2·464 mmol Zn/l from the above-mentioned four Zn sources for up to 30 min. The uptake kinetics of Zn followed a saturable carrier-mediated process. The maximum absorption velocity values were in the following order: control<ZnSO4<Zn-AA W<Zn-Pro M<Zn-Pro S. Moreover, MT mRNA levels for Zn-Pro S (P<0·03), Zn-Pro M (P>0·05) and Zn-AA W(P<0·04) were higher than those for ZnSO4. These findings indicate that organic Zn absorption (especially Zn-Pro S) in intact living broilers was more effective than that of inorganic Zn; organic Zn absorption in the ligated duodenal segment was a saturable carrier-mediated process similar to that of ZnSO4. Moreover, except for MT, there might be other Zn transporters involved in Zn absorption that are affected by different Zn sources.

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