scholarly journals Evaluation of blood chemical, lipids profile and immune response on broiler chicks fed with milk thistle (Silybum mari- anum L.) and thyme (Thymus vulgaris L.) seeds in south-eastern Iran

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Hossein Ansari Nik ◽  
Omid Fani Makki ◽  
Ahmad Ebrahimzadeh ◽  
Arash Omidi
Keyword(s):  
Immune Response ◽  
Total Protein ◽  
Control Diet ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Lipoprotein Cholesterol ◽  
Thymus Vulgaris ◽  
Milk Thistle ◽  
Broiler Chicks ◽  
Lipids Profile

This study was conducted to investigate the effects of supplementation of milk thistle seeds (MTS) and thyme seeds (TS) on blood chemical, lipid profile and immune response in broiler chicks. In this study, 160 one-day-old chicks (Ross 308) were allocated to four treatments with four replicates based on a completely randomized design in a 2×2 factorial arrangement. The treatment groups were (A) basal control diet, (B) basal diet with 0.2 g/kg of MTS, (C) basal diet with 0.2 g/kg of TS and (D) basal diet with 0.2 g/kg of MTS and 0.2 g/kg of TS. Birds fed the (D) supplemented treatment (MTS plus TS) had the greatest levels of total protein, 4.26±0.27 g/L; albumin, 2.21±0.02 g/L; globulin 2.28±0.23 g/L; and aspartate aminotransferase (AST) activity, 152.18±4.46 U/L than the control birds [group (A)] (P<0.05). MTS alone or in combination with TS reduced the cholesterol in the serum of the broilers (P<0.05), and this effect was more pronounced for the (C) treatment (TS alone) (P<0.05). Treatment consumption with MTS plus TS [group (D)] significantly increased the concentration of high-density lipoprotein cholesterol (HDL-C), 90.32±2.28 mmol/L, but low-density lipoprotein cholesterol (LDL-C), 10.44±0.07 mmol/L, and triglyceride concentrations, 60.75±2.65 mmol/L, were decreased compared to control (P<0.05). However, none of the immunity parameters and liver enzymes differed significantly in MTS or TS groups. The present research indicated that supplements of MTS and TS have a protective influence on the lipids profile, total protein, globulin, albumin and AST levels in broiler chicks.

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

Effect of dietary probiotic, antibiotic or combination on broiler performance, cecum microbial population and ileal development

2020 ◽  
Vol 21 (3) ◽  
pp. 74-79
Author(s):  
Ahmed Elbaz ◽  
Said El-sheikh
Keyword(s):  
Control Diet ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Gut Health ◽  
Feed Conversion ◽  
Broiler Performance

Objective: To investigate the effect of antibiotics and/or probiotics on broiler performance, some serum metabolites, cecum microflora composition, and ileum histomorphology under the Egyptian conditions. Design: Randomized controlled experimental study. Animals: Two hundred forty 1-day-old Ross (308) chicks were reared till 35 days of age. Procedures: The birds were randomly allocated into four main groups: a control diet without additives (CON); probiotic (Lactobacillus acidophilus) supplemented diet (PRO); antibiotic (Avilamycin) supplemented diet (ANT) and a mix group (AP) that received antibiotic in the diet form 1 to 4 days of age and treated during the rest of the experimental period with probiotics. Results: Chickens fed on probiotic or antibiotic diets had linear improvement in live body weight (LBW) and feed conversion ratio (FCR) compared with the control group, while the best LBW and FCR were in the AP group. An improvement in the nutrient digestibility was observed in the probiotic added groups (PRO and AP). Serum cholesterol and low-density lipoprotein cholesterol contents decreased when antimicrobial (probiotic or antibiotic) supplementations were used, while there was an increase in high-density lipoprotein cholesterol contents, serum total protein, and albumin levels. Among all groups, cecum Clostridium perfringens and Escherichia coli counts decreased; however, there was an increase in Lactobacillus count compared to the control group. In probiotic supplemented groups (PRO and AP), a significant (P<0.05) improvement in ilea architecture. Conclusion and clinical relevance: Using probiotic after initial treatment with an antibiotic in broiler diets had a positive effect on broiler growth performance, gut health (improved cecum microbial populations and ileum histomorphology), and nutrient digestibility.

scholarly journals Effects of Corn Supplementation into PKC-Urea Treated Rice Straw Basal Diet on Hematological, Biochemical Indices and Serum Mineral Level in Lambs

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 781
Author(s):  
Osama Anwer Saeed ◽  
Awis Qurni Sazili ◽  
Henny Akit ◽  
Abdul Razak Alimon ◽  
Anjas Asmara Samsudin
Keyword(s):  
Rice Straw ◽  
Hematological Parameters ◽  
Control Diet ◽  
Low Density Lipoprotein ◽  
Palm Kernel ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Hematological And Biochemical Parameters ◽  
Experimental Treatments ◽  
Biochemical Indices

Twenty-seven Dorper lambs were used to determine the effect of supplementing corn as a source of energy into the palm kernel cake (PKC) urea-treated rice straw basal diet on the blood metabolic profile and metals in lambs. The lambs were randomly allotted to three experimental treatments according to their initial body weight for a 120 day trial. Dietary treatments were: T1 (control diet) = 75.3% of PKC + 0% corn, T2 = 70.3% of PKC + 5% corn, and T3 = 65.3% of PKC + 10% corn. The results of this study indicated that copper (Cu), selenium (Se), zinc (Zn), and iron (Fe) concentration intake, retention, and its absorption from the gut and apparent mineral digestibility were highly significant for the levels of corn supplementation. The biochemical and hematological parameters remained within normal levels with the treatments, but the white blood cell, eosinophil count, cholesterol, and low-density lipoprotein cholesterol (LDL) were significantly higher in T3. Treatment 3 significantly increased the concentration of Se and Fe, while Zn was reduced in the blood serum of lambs on day 120. The result shows that the inclusion of corn has no effect on the hematological and biochemical parameters of lambs after incorporating corn into the PKC-based diet at 5% and 10%.

scholarly journals Haematological and serum biochemical indices of broiler chickens fed roselle seed (Hibiscus sabdariffa l.) cake based diet as replacement for soyabean meal

2020 ◽  
Vol 46 (2) ◽  
pp. 158-163
Author(s):  
D. N. Onunkwo ◽  
J. C. Ezike ◽  
W. Amaduruonye ◽  
J. Nathaniel ◽  
G. Daniel-Igwe,
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Density Lipoprotein ◽  
Dietary Treatment ◽  
Soya Bean ◽  
Broiler Chicks ◽  
Seed Cake ◽  
Blood Biochemical ◽  
Serum Biochemical ◽  
Dietary Treatments

Feeding trial experiment was carried out to evaluate the haematological and serum biochemical parameters of broiler chickens fed graded levels of Roselle Seed Cake (RSC) as replacement of a conventional protein source. Proximate composition was done to determine the nutrient content of RSC and experimental diets. The experiment contained graded levels of RSC replacing soya bean cake (SBC). One hundred and twenty (120) day old Ross broiler chickens were used in a Completely Randomized Design (CRD). The broiler chicks were assigned to five dietary treatments with three replicates each with 8 birds per replicate and 24 birds per treatment. Five isonitrogenous and isocaloric diets were formulated for the broiler straight diet. Diet 1 served asthe control diet while diets 2, 3, 4 and 5 contained 8%, 16%, 24% and 32% RSC respectively. Data were collected and analyzed using analysis of variance and the mean was separated using Duncan multiple range test. The findings from the study showed that there were no significant (P?0.05) differences in all the parameters measured for haematology across dietary treatment means except for the white blood cell. The blood biochemical composition showed no significant (P?0.05) differences in total protein, high density lipoprotein, low density lipoprotein and creatinine across dietary treatments. RSC as an alternative feedstuff can replace soya bean cake at 24% in the diet of broiler chickens for improved haematological and blood biochemical of broilers. Roselle seed cake can replace SBC at the broiler chicken straight ration at 75% (24% dietary RSC) inclusion for better performance.

scholarly journals Effects of cassava meal on carcass characteristics and cholesterol of growing pig

2020 ◽  
Vol 46 (2) ◽  
pp. 150-157
Author(s):  
M. A. Adeyemi ◽  
E. O. Akinfala
Keyword(s):  
Control Diet ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Growing Pigs ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Leaf Meal ◽  
Enhanced Production ◽  
Cassava Plant ◽  
Dietary Treatments

Twenty growing pigs with an average initial weight of 20.00 + 0.5 kg were randomly assigned to five dietary treatments in a completely randomized design with two replicates per treatment and two animals in each replicate. Cassava Plant Meal (CPM) prepared from sundried unpeeled cassava tubers, leaves and tender cassava stems, mixed at ratio 3:1 of unpeeled tuber meal to cassava leaf meal plus tender stem meal with the ratio of cassava leaf meal to tender stem meal as 5:1 was included in balanced rations at graded levels of 0, 25, 50, 75 and 100 percent in a fifty-six days feeding trials. The experimental pigs were weighed before and after slaughtering, dressing percent was evaluated and carcass length and back fat depth were measured on treatment basis. Meat samples were collected at the ribs (2nd and v3rd and also 12th and 13th) for cholesterol analysis. The 50 % CPM diet produced the heaviest slaughter weight. There was no significant (p >0.05) difference in the dressing percentage of pigs across dietary treatments although pigs on the control diet dressed well while pigs on 100 % CPM dressed the poorest. The 100 % CPM diet induced the leanest carcass. All the carcass and organ measurements were significantly (p <0.05) affected by dietary treatments. There was non-significant (p >0.05) influence of dietary treatments on total and high density lipoprotein cholesterol although the 50 % CPM diet produced the least and highest values respectively. Low density and very low density lipoprotein cholesterol were significantly (p <0.05) affected by dietary treatments. Replacement of maize by varying levels of cassava plant meal diets enhanced production of leaner carcass.

scholarly journals Antidiabetic effect of hydro-methanol extract of Prunus cerasus L fruits and identification of its bioactive compounds

2021 ◽  
Vol 18 (3) ◽  
pp. 597-602
Author(s):  
Gong Xiao ◽  
Xiangcheng Xiao
Keyword(s):  
Total Protein ◽  
Methanol Extract ◽  
Density Lipoprotein ◽  
Prunus Cerasus ◽  
Lipoprotein Cholesterol ◽  
Fruit Extract ◽  
C Peptide ◽  
Antidiabetic Effect ◽  
Total Hemoglobin ◽  
Vitamins E And C

Purpose: To investigate the antidiabetic effect of hydro-methanol extract of Prunus cerasus fruit extract. Methods: The antidiabetic activity was assessed in alloxan-induced diabetic rats. The effect of P. cerasus fruit extract on plasma fasting blood glucose (FBG), insulin, C-peptide, total protein, glycosylated hemoglobin (HbA1c), total hemoglobin, reduced glutathione (GSH), vitamins E and C, ceruloplasmin, lipid profile, histology of the pancreas, and expression of glucose transporter type 4 (GLUT-4) were determined using standard procedures. Liquid chromatography-mass spectrometry was used for phytochemical analysis. Results: Alloxan-induced diabetes significantly reduced plasma levels of insulin, C-peptide, total hemoglobin and total protein, and significantly increased FBG and HbA1c levels (p < 0.05). However, after treatment with the extract, changes in the levels of these parameters were significantly and dosedependently reversed (p < 0.05). The extract also increased the levels of GSH, vitamins E and C, and. Alloxan-induced DM significantly increased the levels of triacylglycerols (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), and significantly reduced the level of total cholesterol (TC) at different time points (p < 0.05). However, after treatment, the levels of TG, LDL-C and HDL-C declined but TC level was significantly elevated time- and dose-dependently by the extract (p< 0.05). The extract upregulated the expression of GLUT-4 mRNA in soleus muscle and adipose tissue. LC-MS analysis revealed that the extract contained chlorogenic acid, rutin, diadzin, amygdalin, quercetin and naringenin. Conclusion: The results obtained in this study have shown that hydromethanol extract of P. cerasus fruits exhibits remarkable antidiabetic effects.

scholarly journals Assessment of lipid profile in-patients with Nephrotic syndrome in Kano Metropolis

2021 ◽  
Vol 14 (1) ◽  
pp. 251-257
Author(s):  
S.Y. Isah ◽  
S.L. Abubakar ◽  
S.B. Danladi ◽  
B.A. Gwaram ◽  
M. Akram ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Serum Creatinine ◽  
Healthy Volunteers ◽  
Lipid Profile ◽  
Total Protein ◽  
Statistical Significance ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Bromocresol Green ◽  
Female Ratio

Nephrotic syndrome (NS) is a complex kidney disease associated with numerous complications which can subsequently lead to cardiovascular disease among others. This study was aimed at assessing the lipid profile, serum and urinary proteins of patients with Nephrotic syndrome (NS) in Kano metropolis. A total of 50 NS patients and 25  apparently healthy volunteers(controls) were recruited for the study, made up of 32 males and 18 females with the age range of 4-70 years. Blood and urine sample were collected from the participants. Serum urea and creatinine were determined using urease  berthelot’s reaction and Alkaline picrate methods. Serum total protein and albumin were assayed using Biuret and bromocresol green binding method through the manual colorimetrictechnique. Serum lipid profile were measured by an enzymatic spectrophotomeric method and the precipitation enzymatic method was specifically used for evaluating the levels of high-density lipoprotein cholesterol (HDL-C). Urine protein was determined using sulphur salicylic acid test. SPSS software package version 21 was used for the analysis of data. High frequency of NS of 40(80%) was observed in patients of <18 years while patient of >46 years had a lower frequency of 4(8%). Males recorded higher frequency of 32(64%) and the frequency of NS among females was 18(36%), thus the male to female ratio for NS was 1.78:1. The mean values of serum creatinine, urea, urinary protein (UP), total cholesterol (T.C), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), T.C/HDL-C, TG/HDL-C, LDL/HDL-C ratio were significantly higher (p<0.05) in patients with NS than the healthy volunteers. Total protein (TP), albumin (Alb), globulin, HDL-C ratio were significantly lower (p<0.05) in NS when compared to healthy volunteers. There was positive correlation between UP and TC, TG and LDL, however, negative correlation was observed between UP and HDL-C with no statistical significance. Dyslipidemia, decreased serum protein and increase serum creatinine, Urea as well as UP were associated with NS. In conclusion, lipid profile and UP analysis may be a useful tool for diagnosis of NS and early diagnosis can reduce the disease morbidity.

scholarly journals The Effect of Ellagic Acid on Hepatic Lipid Metabolism and Antioxidant Activity in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Qiuying Xu ◽  
Shuwei Li ◽  
Wenjie Tang ◽  
Jiayou Yan ◽  
Xiaolan Wei ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Lipid Metabolism ◽  
Antioxidant Capacity ◽  
Ellagic Acid ◽  
Density Lipoprotein ◽  
Basal Diet ◽  
Lipoprotein Cholesterol ◽  
Heme Oxygenase 1 ◽  
Hepatic Lipid Metabolism ◽  
Hepatic Lipid

Accumulating evidence has demonstrated that the imbalance of lipid metabolism and antioxidant capacity leads to damage to liver. The present study aimed to investigate the effects of ellagic acid (EA), a phenolic compound, on hepatic lipid metabolism and antioxidant activity in mice. In our study, 24 C57BL/6J mice were divided into three groups: (1) control (CON); (2) basal diet+0.1% EA (EA1); and (3) basal diet+0.3% EA (EA2). After the 14-day experiment, the liver was sampled for analysis. The results showed that 0.3% EA administration increased the liver weight. Total cholesterol and low-density lipoprotein cholesterol activities decreased and high-density lipoprotein cholesterol activity increased by EA supplementation. Meanwhile, dietary supplementation with EA dose-dependently decreased the acetyl-CoA carboxylase protein abundance and increased the phospho-hormone-sensitive lipase, carnitine palmitoyltransferase 1B, and peroxisome proliferator-activated receptor alpha protein abundances. Moreover, EA supplementation reduced the malonaldehyde concentration and increased the superoxide dismutase and catalase concentrations. The protein abundances of phospho-nuclear factor-E2-related factor 2, heme oxygenase-1, and NAD(P)H: quinone oxidoreductase 1 increased by EA supplementation in a dose-dependent manner. Taken together, EA supplementation promoted the lipid metabolism and antioxidant capacity to maintain the liver health in mice.

249 BIOPHYSICAL AND BIOCHEMICAL CHARACTERISTICS OF ALPACA SEMEN AFTER COLLECTION BY ELECTROEJACULATION

2013 ◽  
Vol 25 (1) ◽  
pp. 272 ◽  
Author(s):  
J. Rodriguez ◽  
W. Huanca ◽  
M. Ramos ◽  
M. Vasquez ◽  
J. Espinoza
Keyword(s):  
Alkaline Phosphatase ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Density Lipoprotein ◽  
Alanine Transaminase ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Biochemical Characteristics

The collection of alpaca semen presents difficulties because of the characteristics of mounts and copulation time in addition to the high viscosity of semen. The biophysical and biochemical characteristics of alpaca semen were investigated. Semen samples from four adult males from 6 to 7 years of age were collected using an electroejaculation technique. The biophysical parameters measured in seminal plasma included volume, color, sperm concentration, motility, and viscosity, and the biochemical parameters included pH and glucose, calcium, triglyceride, cholesterol, high-density lipoprotein cholesterol, total protein, albumin, alanine transaminase, alkaline phosphatase, and γ-glutamyltranspeptidase concentrations. The mean time for semen collection was 10.3 ± 1.5 min; the volume varied from 1.2 to 3.8 and the colour from clear to milky, and pH was 7.4 ± 0.03. Mean concentration of spermatozoa was 69.3 ± 193 million mL–1, and motility was 52.4 ± 9.7%. Biochemical parameters were glucose (mg dL–1) 8.22 ± 0.77; cholesterol (mg dL–1) 79.78 ± 5.64; triglycerides (mg dL–1) 44.12 ± 7.38; high-density lipoprotein cholesterol (mg dL–1) 4.73 ± 0.30; total protein (g dL–1) 2.36 ± 0.15; albumin (g dL–1) 0.97 ± 0.33; calcium (mg dL–1) 11.77 ± 1.74; alanine transaminase (U L–1) 17.92 ± 9.09; alkaline phosphatase (U L–1) 288.76 ± 279.59; and γ-glutamyltranspeptidase (U L–1) 89.62 ± 39.09. These results provide information on the biophysical and biochemical characteristics of alpaca semen collected by electroejaculation, with variable results in the enzymatic components.

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