Effect of probiotics mix supplementation on haemato-biochemical parameters and bacterial faecal shedding in Corriedale lambs fed paddy straw based complete feed

2018 ◽  
Author(s):  
G. G. Sheikh ◽  
Danish Masood ◽  
A. M. Ganai ◽  
S. Muzamil ◽  
Y. Afzal ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Haemoglobin Concentration ◽  
Hdl Cholesterol ◽  
Total Coliform ◽  
Total Serum Protein ◽  
Total Serum ◽  
Monocyte Count ◽  
Paddy Straw ◽  
Faecal Consistency

Growth trial of 90 days was conducted on 12 male Corriedale lambs, divided in two groups of six lambs each, to study the effect of feeding of paddy straw based complete feed supplemented of probiotic mix (Saccharomyces cerevisiae 2×1010cfu/g + Lactobacillus acidophilus 6×109cfu/g) in equal ratio @ 3 % of DM, as per the in vitro studies, while complete feed without probiotics served as control. There was significantly (P less than 0.01) higher haemoglobin concentration, RBC, WBC and monocyte count in animals fed probiotic mix than control, with no significant effect on PCV, lymphocyte, eosinophill and neutrophill count. Blood glucose, total serum protein, albumin, globulin, BUN and serum creatinine values were statistically higher in probiotic mix supplemented animals. Lambs fed with probiotics show significant (P less than 0.01) decrease in serum cholesterol, LDL-cholesterol and triglycerides with increment of HDL-cholesterol than animals fed unsupplemented diet, with no effect on enzyme activities of liver enzymes. The lambs fed on probiotic mix shows significant reduction in faecal shedding of E. coli and total coliform with firm pellets faecal consistency. Probiotic mix has great potential to beneficially affect the haemato-biochemical parameters and gut micro-flora and hence improve physiological health status of animals.

scholarly journals Studies on the toxicity of an aqueous extract of the leaves of Abrus precatorius in rats

2007 ◽  
Vol 74 (1) ◽  
Author(s):  
A.A. Adedapo ◽  
O.A. Omoloye ◽  
O.G. Ohore
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Aqueous Extract ◽  
Haemoglobin Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Cell Count ◽  
Abrus Precatorius ◽  
White Rats ◽  
Histopathological Studies

The toxic effects of an aqueous extract of Abrus precatorius were studied in 20 male white rats over a period of 18 days. The rats were divided into four groups of five rats per group. Those in Group A served as controls while the rats in Groups B, C and D were dosed per os with 400 mg/kg, 800 mg/kg and 1 600 mg/kg of the extract, respectively. Blood samples were collected for haematological and biochemical analysis and specimens of the liver, kidney and testes were taken for histopathological studies. The study showed that the extract of A. precatorius caused decreased levels of packed cell volume, haemoglobin concentration, red blood cell count, white blood cell count, mean corpuscular volume and mean corpuscular haemoglobin. The extract also resulted in increased levels of total serum protein, albumin, alanine amino transaminase, aspartate amino transferase, alkaline phosphatase and total bilirubin. Histologically, testicular degeneration characterized by decreased numbers of lining cells of the epithelium as well as reduction in sperm cells with presence of scattered Sertoli cells were noted. The study thus showed that aqueous extract of Abrus precatorius is toxic and caution should be exercised in its use for medicinal purpose.

Haematological and serological response of broiler chickens to varying levels of direct fed microbes as feed additive

2020 ◽  
Vol 47 (3) ◽  
pp. 90-98
Author(s):  
D. N. Onunkwo ◽  
O. A. Ekine
Keyword(s):  
Blood Cell ◽  
Broiler Chickens ◽  
Haemoglobin Concentration ◽  
Feed Additive ◽  
Total Serum Protein ◽  
Total Serum ◽  
Broiler Chicks ◽  
Serological Response ◽  
Serum Chemistry ◽  
Intestinal Immunity

Several efforts are being made to find the possibilities of utilizing Direct fed microbes (DFM) that has a potential to replace antibiotics as a result of its ability to prevent internal colonization of enteropathogenic enzymes, stimulate intestinal immunity of broiler chickens and reduce stress in animals. A studywas conducted using 120, one day-old Ross 308 broiler chicks to evaluate blood and serum chemistry response to direct fed microbes. The broiler chicks were obtained from a reputable hatchery and randomly assigned to four dietary treatments, each with three replicates of ten birds per treatment. The treatments were T1, T2, T3 and T4, with levels of inclusion of direct fed microbes (DFM) at 0, 2.5, 5% and 7.5%, respectively. The birds were assigned to these treatment diets, feed and water was given ad libitum throughout the duration of the experiment which lasted for 56 days. The experimental design was Completely Randomized Design (CRD). At the end of the experiment blood samples were collected from the chickens and taken to the laboratory for haematological and serum chemistry analysis. The haematological characteristics of the broiler chickens showed that there was no significant (P>0.05) in haemoglobin (Hb), Packed Cell Volume (PCV), Red Blood Cell (RBC), White Blood Cell (WBC), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH) and Mean Cell Haemoglobin Concentration (MCHC). The WBC portrayed decreasing trend as the level of DFM increased, also the Hb, MCV and MCH portrayed an increasing trend as the DFM increased in the treatment diet up to the 5% inclusion level. The serum chemistry result shows that there were significant differences (P<0.05) in total serum protein and globulin whereas no significant differences (P>0.05) were observed in serum albumin, glucose, urea and creatinine. It could be concluded that fed diet 2 containing 2.5% DFM performed better as they compete favourably with the control. Broiler chickenss fed diet 2 also had a balanced physiological system

Controlling the Physical Behavior and Biological Performance of Liposome Formulations Through Use of Surface Grafted Poly(ethylene Glycol)

2002 ◽  
Vol 22 (2) ◽  
pp. 225-250 ◽  
Author(s):  
C. Allen ◽  
N. Dos Santos ◽  
R. Gallagher ◽  
G.N.C. Chiu ◽  
Y. Shu ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Protein Binding ◽  
Chemical Properties ◽  
Total Serum Protein ◽  
Total Serum ◽  
Poly Ethylene Glycol ◽  
Binding Studies ◽  
Poly Ethylene

The presence of poly(ethylene glycol) (PEG) at the surface of a liposomal carrier has been clearly shown to extend the circulation lifetime of the vehicle. To this point, the extended circulation lifetime that the polymer affords has been attributed to the reduction or prevention of protein adsorption. However, there is little evidence that the presence of PEG at the surface of a vehicle actually reduces total serum protein binding. In this review we examine all aspects of PEG in order to gain a better understanding of how the polymer fulfills its biological role. The physical and chemical properties of the polymer are explored and compared to properties of other hydrophilic polymers. An evidence based assessment of several in vitro protein binding studies as well as in vivo pharmacokinetics studies involving PEG is included. The ability of PEG to prevent the self-aggregation of liposomes is considered as a possible means by which it extends circulation longevity. Also, a “dysopsonization” phenomenon where PEG actually promotes binding of certain proteins that then mask the vehicle is discussed.

scholarly journals Lamivudine-Induced Liver Injury

2015 ◽  
Vol 3 (4) ◽  
pp. 545-550 ◽  
Author(s):  
Lamidi W. B. Olaniyan ◽  
Emmanuel N. Maduagwu ◽  
Olalekan Wasiu Akintunde ◽  
Oladimeji O. Oluwayelu ◽  
Bartholomew I. C. Brai
Keyword(s):  
Protein Concentration ◽  
Female Rats ◽  
Total Serum Protein ◽  
Total Serum ◽  
Target Tissue ◽  
Liver Sinusoid ◽  
Hydropic Degeneration ◽  
Lymphoid Cell Population ◽  
Histopathological Studies

BACKGROUND: Lamivudine is a nucleoside analogue antiretroviral drug, known for its low toxicity at clinically prescribed dose. However, the toxicity or mechanism of toxicity and target tissue effects during prolonged administration of higher doses were hardly given sufficient laboratory attention.AIM: The present work was designed to investigate the biochemical and histopathological changes in the liver of rat administered with prolonged doses of lamivudine.MATERIAL AND METHODS: Lamivudine in multiple doses of five ranging from 4 mg/kg to 2500 mg/kg were administered, in vitro, by injection into the air-sac of 10–day old fertile embryonated eggs of Gallus domesticus. Also, female rats of the Wistar strain received oral doses, up to 500 mg/kg singly or repeatedly for 15 or 45 days, respectively. Spectrophotometric techniques were employed to monitor activities of the aminotransferases (ALT and AST), γ–glutamyltransferase (GGT) and total protein concentration in serum while activities of glutathione S–transferase (GST), GGT and superoxide dismutase (SOD) as well as concentrations of malondialdehyde (MDA) and protein were determined in liver. Histopathological studies were carried out on liver. Data were analysed using ANOVA and were considered significant when p < 0.05.RESULTS: The LD50 for the drug calculated from the incubation experiment was 427 mg/kg. Total serum protein concentration significantly reduced while enzymes activities significantly increased at 500 mg/kg only among the repeat-dosed rats. Hepatic GGT, GST and SOD activities as well as MDA concentration were significantly elevated at 20 mg/kg. Histopathological studies showed multifocal lymphoid cell population in the liver sinusoid of the chicken and hydropic degeneration of hepatocytes were recorded among rats repeatedly exposed to the drug respectively at doses ≥ 100  mg/kg.CONCLUSION: Lamivudine toxicity in rat liver appeared to be mediated by oxidative stress.

Metabolic changes induced by regular submaximal aerobic exercise in meat-type rabbits

2003 ◽  
Vol 51 (4) ◽  
pp. 503-512 ◽  
Author(s):  
Éva Szabó ◽  
R. Romvári ◽  
P. Bogner ◽  
Hedvig Fébel ◽  
Zs. Szendr'
Keyword(s):  
Venous Blood ◽  
Hdl Cholesterol ◽  
Serum Cortisol ◽  
Creatine Kinase Activity ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Lactate Dehydrogenase Activity ◽  
Activity Measurements ◽  
Submaximal Aerobic Exercise

Pannon White growing rabbits (a group of 8) were exposed to treadmill exercise (3-9 m/s, 1.2-1.6 km/day) twice a day for 4 weeks, while additional 8 animals, kept inactive, were assigned as the control group. Weekly, 12 hours after exercise, venous blood was taken for serum metabolite and enzyme activity measurements. Total serum protein, albumin and creatinine levels significantly increased during the second half of the training, as compared to the control group. Triacylglycerol levels in the exercised group as compared to controls, however, were higher only after the first and the fourth weeks of the experiment. Resting non-esterified fatty acid (NEFA) concentration of the trained rabbits was lower at the end of the trial. On the other hand, there were no significant differences, as compared to the respective controls, in serum urea, total and HDL cholesterol levels. At the end of the exercise alkaline phosphatase activity was higher and total lactate dehydrogenase activity was lower in the trained rabbits. Serum alanine aminotransferase, aspartate aminotransferase and γ-glutamyl transpeptidase activities were not changed, while creatine kinase activity was slightly lower in the trained group. The serum cortisol concentration was not different in the trained and control rabbits.

scholarly journals The short-term prognosis of severe primary infantile malnutrition

1967 ◽  
Vol 21 (1) ◽  
pp. 155-165 ◽  
Author(s):  
J. S. Garrow ◽  
M. C. Pike
Keyword(s):  
Serum Bilirubin ◽  
Haemoglobin Concentration ◽  
Skin Lesions ◽  
Multiple Correlation Coefficient ◽  
Total Serum Protein ◽  
Total Serum ◽  
Bilirubin Concentration ◽  
Liver Size ◽  
Rate Of Recovery ◽  
Serum Bilirubin Concentration

1. Among a series of 343 children with severe primary malnutrition there were 248 for whom, on admission, there were reliable records of age, weight, height, liver size, severity of oedema, skin lesions and angular stomatitis, and concentration of total serum protein, haemoglobin and sodium. For eighty-four of these children the serum bilirubin concentration was also known.2. The correlations of these characteristics of the children on admission, with mortality, and with the rate of recovery were investigated.3. Age, weight, oedema and haemoglobin concentration were not significantly related to mortality or to rate of recovery. A multiple regression analysis showed that an increased serum bilirubin concentration and a decreased serum sodium concentration indicated a bad prognosis, and these two factors contributed almost the whole of the multiple correlation coefficient of 0.63 with respect to mortality and 0.59 with respect to speed of rccovery.4. We conclude that in our series of children death was more closely associated with liver failure or overhydration than with protein depletion, and suggest that the administration of ton much protein or water to an acutely ill malnourished child may precipitate death.5. This analysis shows that the mortality observed in our series cannot be adequately explained by any combination of the characteristics considered above; there must, therefore, be other factors of importance for which we do not have suitable measurements.

scholarly journals Comparison of some serum biochemical parameters between lactating and non-lactating dairy cows in selected dairy farms of Chittagong district of Bangladesh

2015 ◽  
Vol 1 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Md Samun Sarker ◽  
Md Ahaduzzaman ◽  
Md Abu Sayeed ◽  
Rajib Sarker ◽  
Minhazul Abedin Nanno ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Laboratory Data ◽  
Dairy Farms ◽  
Total Serum Protein ◽  
Total Serum ◽  
Physiological Status ◽  
Phosphorus Concentration ◽  
Serum Biochemical Parameters ◽  
Lactating Cows ◽  
Serum Biochemical

Physiological status-related changes in serum biochemical values are known to occur in many species. The aim of the current study was to evaluate total serum protein, albumin, glucose, calcium and phosphorus concentration and these parameters were measured by the serum analyzer for lactating (n=50) and non-lactating (n=50) dairy cattle in selected dairy farms of Chittagong district. The results (mean ± standard deviation) of the study showed that among serum biochemical parameters total protein (82.3 ± 2.15 gm/l), albumin (27.45 ± 5.06 gm/l), calcium (12.97±6.67 mg/dl) and phosphorus (7.26 ± 0.41 mg/dl) level in non-lactating cows were higher than the value of lactating cows as (81.28 ± 2.41 gm/l), (14.09 ± 2.05 gm/l), (10.05 ± 2.51 mg/dl) and (6.52 ± 0.30 mg/dl) respectively. Only glucose level in lactating cows (63.01 ± 6.67 mg/dl) was found higher than the non lactating cows (58.47 ± 3.84 mg/dl). In case of parity-wise parameters insignificant variation was noticed. It may be concluded that biochemical variation present between lactating cow and non lactating cow is important for interpretation of laboratory data and also for physiological diagnosis.Asian J. Med. Biol. Res. June 2015, 1(2): 259-264

scholarly journals Correlation between malondialdehyde and metanephrine in patients with acute lymphoblastic leukemia

2014 ◽  
Vol 11 (3) ◽  
pp. 1217-1225
Author(s):  
Baghdad Science Journal
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Serum Level ◽  
Blood Cell ◽  
Biochemical Parameters ◽  
Lymphoblastic Leukemia ◽  
Serum Glucose ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Cell Count ◽  
Healthy Control

Acute lymphoblastic leukemia (ALL) is one of the most common diseases , so in this study the serum level of malondialdehyde and its relationship with metanephrine was investigated in acute lymphoblastic leukemia patients over one month of treatment. Some biochemical parameters (serum glucose , total serum protein , malondialdehyde ,vitamin C, and metanephrine) changed as well as white blood cell count and blood hemoglobinlevelswere analyzed in sixty patients diagnosed with acute lymphoblastic leukemia over one month of treatment compared to healthy control group.Statistically significant increases (p

scholarly journals Evaluation of some biochemical changes in the serum of phensedyl addicts of Gaibandha District, Bangladesh

2014 ◽  
Vol 20 ◽  
pp. 57-65
Author(s):  
Md Rashidul Hasan ◽  
Parvez Hassan ◽  
Md Abdul Jalil Miah
Keyword(s):  
Serum Cholesterol ◽  
Biochemical Parameters ◽  
Ldl Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Triglyceride ◽  
Hdl Cholesterol ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
P Value

Context: Abuse of the drug, Phensedyl like any other drug might exert adverse effects on vital organs of th e h u m an body like liver, kidney and heart. Objectives: To determine the effects of Phensedyl intake on the serum biochemical parameters of the addicts in order to access for damages of vital human organs like liver, kidney and heart. Materials and Methods: Study population consisted of 127 male Phensedyl addicts within the ages of 18–55 years of defined criteria from Gaibandha district, a Northern part of Bangladesh, during July 2009 to December 2011. Fifty (50) non-drug dependent healthy men of matched age, height, and socioeconomic status were included as controls from the same community. Biochemical parameters analyzed were – Serum creatinine, SGOT, SGPT and Lipid profiles (total serum cholesterol (TC), Serum triglyceride (TG), serum high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol determined by semiautomatic biochemistry analyzer using commercially available kits Results: Abuse of Phensedyl appears not to hamper the normal renal and liver function in most of the addicts even after 8 years of Phensedyl intake irrespective of age except in case of 4 abusers. Serum total cholesterol (TC) remained almost unchanged among the addicts abusing Phensedyl for less than 8 years. But very strikingly, addicts taking Phensedyl for more than 8 years had higher trends in serum cholesterol i.e. more than 200 ml/dl. Of the addict’s, 44.36% abusing Phensedyl for less than 8 years had normal triglyceride (TG) values whereas, in 45.66% addicts abusing the drug for more than 8 years had clinically very significantly elevated triglyceride levels, which was also found to be statistically very significant (p value =0.0001), indicating the risk of developing cardiac diseases. Of the total addicts 53.53% had low levels of HDL cholesterol, which is clinically and statistically found to be very significant (p value =0.002). Of the addict’s 43% taking the drug for less than 8 years had normal LDL levels but significantly elevated values were recorded in 34% of the abusers who had been taking the drug for more than 8 years. Conclusion: Long time (> 8 years) Phensedyl abusers are at the high risk of developing Brain stroke, Coronary Heart Disease (CHD), Ischemic stroke or transient ischemic attack (TIA) as there is the triad of: Elevated LDL cholesterol, Low HDL cholesterol and elevated Triglyceride. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17656 J. bio-sci. 20: 57-65, 2012

Intestinal Ammonia in Uraemia: The Effect of a Urease Inhibitor, Acetohydroxamic Acid

1972 ◽  
Vol 42 (1) ◽  
pp. 105-112 ◽  
Author(s):  
J. D. Swales ◽  
J. D. Tange ◽  
D. J. Evans
Keyword(s):  
Treated Group ◽  
Ammonia Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Acetohydroxamic Acid ◽  
Urease Inhibitor ◽  
Ammonia Production ◽  
Ammonia Content

1. The effects of intestinal ammonia in uraemia have been studied by administering a urease inhibitor, acetohydroxamic acid, to bilaterally nephrectomized rats. 2. Ammonia concentration was significantly decreased in the colon although it remained slightly higher than in normal animals. Caecal ammonia concentration was not altered. Total ammonia content was decreased in both colon and caecum. 3. A study in vitro indicated that ammonia production could be totally inhibited by acetohydroxamic acid. This suggests that the failure to suppress ammonia production in vivo is due to failure of the inhibitor to reach the site of urease production in sufficient concentration. 4. The animals treated with high doses of acetohydroxamic acid survived for a shorter time. Blood urea was higher and total serum protein lower in the treated group. The incidence of colitis was not affected.

Sign in / Sign up

Export Citation Format

Share Document