scholarly journals Characterization of Selected Jackfruit Germplasm Accessions for Fruit Shape Through Morphological and Marker Based Assay

Author(s):  
Gayatri . ◽  
K. Kavya ◽  
S. Shyamalamma

The study involved characterization of Jackfruit accessions with obloid (round) versus ellipsoid fruit shape. The morphological characterization was based on tree, fruit and seed descriptors. The Jackfruit accessions exhibited wide range of differences. The dominant tree growth habit was erect type, with medium to low branching density and branching pattern was irregular in most of the trees. With respect to fruit rind weight, it was more than fifty to sixty per cent in accessions with obloid fruit, than ellipsoid fruits. The rind weight ranged from thirty to forty per cent of the total fruit weight. Based on fruit and flake quality characters, the accessions such as Allilugatta 5, Kumaraswamy, Manipur Parmesh and Swarna in obloid fruits and Ashoka Red, Ashoka Yellow, Byrachandra, Janagere and NSP in ellipsoid fruits have been identified for commercial purpose. Among ten RAPD primers screened, two primers showed higher PIC value (0.24) viz., OPA 4 and OPAH 4. Similarly among ten ISSR markers screened, ISSR - 868 (0.31) showed higher PIC value. These can be used effectively to probe the genetic variations among the Jackfruit accessions.

2021 ◽  
Vol 3 (5) ◽  
pp. 81-89
Author(s):  
Faruque Ahmed ◽  
Anwar Hossain ◽  
Nasir Uddin Ahmed ◽  
Md. Jahangir Alam ◽  
Md. Shoriful Islam

The research was conducted to evaluate the morphological variability in fruits traits among the mulberry genotypes grown in Mulberry Germplasm Bank of Bangladesh Sericulture Research and Training Institute (BSRTI), Rajshahi, during 2018–2020. The aim of the research was to find out the genotypes accessibility for varietal improvement. Total 50 mulberry genotypes were morphologically characterized on fruit traits through this research. The experimental design was Randomized Complete Block Design (RCBD) with three replications and the plantation system was high bush. Each plot consists of 20 plants and unit plot size was 4 m × 5 m. Fruit colour, fruit taste and seed colour was determined. Nine distinct fruit colours such as reddish-black (23.33%), black-berry (18.33%), cream (10%), black (8.33%), white-cream (6.67%), pink (6.67%), pinkish (3.33%), orange (3.33%) and radish (1.67%) were observed among several germplasms. Remarkable variation was found in fruit taste such as sour sweet (28.33%), sweet (21.67%), light sweet (5%), light-sour sweet (5%) and deep sweet (5%), respectively. Five colored of seed viz: light yellow, light brown, yellowish brown, dark brown and blackish brown were observed among the germplasm. Length of the fruit diverse from 0.73 (BSRM-8) to 5.58 (BSRM-56 and widthfrom 0.52 (BSRM-8) to1.9 (BSRM-56),single fruit weight varied from 0.07 (BSRM-8) to 4.11 (BSRM-56), fruit weight per plant varied from 101.47 (BSRM-11) to 2250.43 (BSRM-56), seed setting (%) varied from 8.13 (BSRM-29) to 94.24 (BSRM-16), sprouting (%) varied from 36.67 (BSRM-22) to 96.67 (BSRM-38), rooting (%) varied from 13.89 (BSRM-22) to 98.33 (BSRM-10),achene number/fruit varied from 12.53 (BSRM-10) to118.10 (BSRM-56), seed number/fruit varied from 2.99 (BSRM-8) to 47.86 (BSRM-56) and 100 seed weight varied from 0.019 (BSRM-35) to 0.166 (BSRM-56), respectively. Results showed that the black-berry, sweet tasted fruited mulberry (BSRM-56), cream colored, sweet tasted fruited mulberry (BSRM-1) and white cream, sweet tasted fruited mulberry (BSRM-34) was promising on the basis of greatest fruit production potential to be appears for further commercial utilization. Generally, a wide range of variation was exhibited among the sampled and characterized genotypes.


2020 ◽  
pp. 1487-1496
Author(s):  
Marline Hanny Owino ◽  
Bernard Mukiri Gichimu ◽  
Phyllis Wambui Muturi

Morphological characterization of genotypes is fundamental in providing information on their genetic status to guide on their conservation and improvement. The objective of this study was to determine agro-morphological diversity within horned melon in Kenya. The study was carried out in two seasons at the University of Embu in Kenya. The study characterized 19 horned melon accessions collected from different agro-ecological zones in Eastern, Central and Western regions in Kenya. The experiment was laid out in a randomized complete block design with three replications. Morphological characterization of the accessions was based on melon descriptors from International Plant Genetic Resources Institute (IPGRI) with slight modifications. Significant differences were observed in all the quantitative traits except the number of branches and main vine length. However, qualitative variations were only observed in fruit shape, rind colour and seed shape. Principle Component Analysis (PCA) showed that fruit weight, main vine length and days to maturity had the highest contribution to the observed diversity. Cluster analysis separated the accessions into seven groups with between classes diversity of 79.20% and within classes diversity of 20.80%. The diversity observed can be exploited by plant breeders for genetic improvement of the crop.


2020 ◽  
Vol 73 (1) ◽  
pp. 9019-9027
Author(s):  
Jorge Andres Betancur G. ◽  
Sandra Bibiana Muriel R. ◽  
Elena Paola Gonzalez J.

The red dragon fruit Selenicereus undatus is a native fruit from the Americas. Its growing appeal in the international market as an exotic fruit highlights the relevance of the crop for the local economies. In Colombia, the high diversity within the genus and species makes it difficult to have reliable materials for propagation as cultivars. This agronomic challenge requires more studies of the distribution and real diversity within the genus Selenicereus sp. Due to the information aforementioned, the fruit is harvested in small localities as an extraction production system. This project aimed to morphologically characterize the working collection of red dragon fruit under growing conditions in the Municipality of San Jerónimo. It contained 30 accessions from the departments of Antioquia, Córdoba, and Valle del Cauca. Twenty-one characters of cladode, flower, and fruit were quantified to assess the diversity, and a restricted cluster analysis was performed to understand the morphological richness of the materials. As a result, it was observed differences in terms of stems, such as distance between areolas and width of cladode. This was also observed in flowers, in which their total length, the width of petals, the presence of trichomes, the color of calyx and petals, and the color of the stigma were different. Also, differences were recorded in the fruit. Characteristics such as weight and color of its skin were assessed, resulting in fruit size medium to small. Regarding other properties, total soluble solids varied between 11.2 to 15.6 °Brix. 55% of the fruits have measurements that fit market requirements. Finally, three accessions match the requirements of weight and °Brix, to be used as possible genotypes by commercial growers; however, studies about agronomic management strategies that favor an increase in fruit weight must be done.


2018 ◽  
Vol 7 (2) ◽  
pp. 89-98 ◽  
Author(s):  
Farzana Alam ◽  
Kazi Didarul Islam ◽  
SM Mahbubur Rahman

The research was conducted for the assessment of genetic diversity using both morphological and random amplified polymorphic DNA (RAPD) analysis of twelve guava (Psidium guajava L.) varieties growing in Bangladesh. Morphological characterization of guava varieties showed a wide range of variation. The highest variability was observed between Poly and Jelly varieties.Polymerase chain reaction with 5 arbitrary 10-mer and 3 arbitrary 12- mer RAPD primers produced a total of 50 bands of which 75.23 percent were polymorphic. The highest percentage of polymorphic loci (100%) was observed for primer A and the lowest (50%) for A03 primer. The UPGMA dendrogram revealed the segregation pattern and the difference of evolutionary changes. Guava varieties were separated into two main groups, one of them was made up of Chineese, Jelly, Kazi, Apple, L-49, Local-2 and Local-3. The other one was made up of Local-1, Poly, Kashi, Thai and Bombay. The highest genetic distance between Apple and Kazi peyara indicate that these varieties might be interesting in breeding programme for improving trait of interest. This scientific information could be used for further improvement of guava. Jahangirnagar University J. Biol. Sci. 7(2): 89-98, 2018 (December)


2020 ◽  
pp. 19-32
Author(s):  
D. R. B. Sonchita ◽  
F. M. Aminuzzaman ◽  
A. A. Joty ◽  
J. F. Tanni ◽  
M. N. Islam ◽  
...  

Survey on macro fungi was made in Gajni forest, Sherpur, Bangladesh which is located in between 24°18' and 25°18' north latitudes and in between 89°53' and 90°91' east longitudes. It is bounded by Meghalaya state of India on the north, Mymensingh and Jamalpur districts on the south with a wide range of ecosystem. The survey was conducted on July to December, 2018 to identify and preserve wood-rot causal macro fungi for future industrial utilization. Morphology of basidiocarp and characteristics of basidiospore were recorded. A total of 20 samples were collected and identified to 12 species belonging 7 families. Dominant species was Ganoderma species. The identified four species were from Ganodermataceae family and these were G. applanatum, G. lucidum, G. tropicum and G. lobetum. Other dominant genus was Russula. Other recorded genera were Hebeloma, Boletus, Phlebopus and Entoloma. Among them the highest frequency (85.72%) was recorded for G. applanatum and lowest frequency (7.14%) was recorded for Phlebopus marginatus. Similarly highest density (20.25%) was recorded for Agaricus sp. followed by G. lucidum (15.85%). The lowest density was (2.14%) was recorded for Phlebopus marginatus. Collected specimens were preserved in Sher-e-Bangla Agricultural University Herberium of Macro fungi (SHMF) for further study.


2019 ◽  
Vol 13 ◽  
pp. 115
Author(s):  
Ana Veruska Cruz Silva ◽  
Ana Letícia Sirqueira Nascimento ◽  
Adrielle Naiana Ribeiro Soares ◽  
Allivia Rouse Carregosa Rabbani ◽  
Josué Francisco Silva Júnior ◽  
...  

The mangabeira (Hancornia speciosa G.) is a native Brazilian species in the process of domestication. It has several potential uses. In recent years the populations of mangabeira have become fragmented and, to conserve their genetic resources, Embrapa Tabuleiros Costeiros has, since 2006, maintained a Mangaba Genebank, which currently has 299 individuals distributed across 27 accessions. In one of these accessions (CP), first fruiting was observed to occur at 13 months, indicating precocity. The current project was developed with the intention of morphologically characterizing the CP accessions and evaluating the extent of genetic diversity between genotypes, based on genetic markers associated with fruiting precocity. Morphological variation in crown and leaf shape, growth habit, total height, stem diameter and canopy height were investigated. For molecular characterization, 18 CP accession genotypes were used, all from Sergipe State, Brazil, and 11 ISSR markers were studied that generated 62 fragments with 91.4% polymorphism. The fruits have physical and chemical characteristics suitable for in natura consumption and also for processing. UPGMA dendrogram analysis showed the genotypes CP2 and CP6 to be most similar and CP10 the most divergent within the CP accessions. The results showed the existence of polymorphism, and genetic divergence among genotypes, thus reinforcing the importance of constant germplasm characterization, which guarantees information on gene sources for future use, while providing information concerning potential parents that can be used in breeding programs as well as the identification of duplicates.


2000 ◽  
Vol 57 (4) ◽  
pp. 791-794 ◽  
Author(s):  
Vicente Manuel Gómez-López

The objective of the present paper was to characterize some avocado fruit varieties to increase the information pool for this fruit. Twelve avocado varieties of medium oil content (8.09-11.12%) from a Venezuelan orchard: Wilson Popenoe, Figueroa 1, Waldin, Puebla, Schaff, Celia, Araira FM, Adolfo, Esencia de la Vega, Lawhon, Winslowson, and Lujo were characterized for pulp oil and moisture; weight (whole fruit, seed, pulp, and peel); length, width, and fruit shape; peel characteristics (roughness, color and hand peeling); and ripeness time. Differences in oil content, moisture content and fruit weight were found when compared with results reported by other authors. The variety Puebla was the lightest (107 g) and the smallest (7.90 x 4.62 cm), while Wilson Popenoe presented the highest pulp proportion (82%). Most of the varieties were ovate, with rough green peel and easy to hand peel. Peak ripeness of some varieties was at 3-12 days. Potential yields of two varieties were higher that the Venezuelan average.


Author(s):  
Stanislava Grozeva ◽  
Amol N. Nankar ◽  
Daniela Ganeva ◽  
Ivanka Tringovska ◽  
Gancho Pasev ◽  
...  

Characterization of local germplasm is an effective way to identify elite breeding material and develop improved varieties. This study was aimed to assess 52 tomato accessions comprised of local varieties (28), landraces (8), breeding lines (14), and wild relatives (2) and its characterization for 30 morphological/agronomic, four fruit quality, and Tomato Mosaic Virus (ToMV) resistance traits. Morphological, quality, and ToMV traits were evaluated using phenotyping, biochemical assays, and molecular markers, respectively. Fruit shape and size showed appreciable variation with fruits varied from rounded to heart shape and small to big size. Significant variation was observed for fruit weight (1.6g to 564.8g), fruits per plant (6.0 to 174.7), productivity (130.5g to 5146.5g), soluble solids (4.1% to 8.4%), vitamin C (9.5 mg/100g to 46.4 mg/100g), antioxidant activity (2.5 μmol Fe2+/g FW to 9.6 μmol Fe2+/g FW), and total polyphenols (23.9 GAE/100g FW to 124.2 GAE/100g FW). All accessions were phenotypically screened for the virus resistance in the growth chamber and CAPS molecular markers were used to identify accessions with ToMV Tm-22 resistant alleles and accessions LYC-13, LYC-15, LYC-17, LYC-26, and LYC-52 were identified as resistant. Multivariate analysis of morphological and quality traits showed that 35 principal components (PCs) contributed to the total variation and the first two and twelve PCs explained 47.2% and 90% variation, respectively. The evaluated tomato collection appears to have breeding potential and around 20% accessions of the collection (LYC-6, LYC 17-18, LYC 26-31, LYC 33) are promising genetic resources for variety development that are enriched with enhanced fruit quality and high yield.


Genetika ◽  
2014 ◽  
Vol 46 (1) ◽  
pp. 199-207 ◽  
Author(s):  
Emina Mladenovic ◽  
Janos Berenji ◽  
Vladislav Ognjanov ◽  
Mirjana Ljubojevic ◽  
Jelena Cukanovic ◽  
...  

The aim of this study is collecting and preserving of unique Cucurbita pepo germplasm in Serbia. This study also describes the intraspecific variation of C. pepo within twenty germplasm accessions. Conservation work aimed to develop a representative core collection of C. pepo germplasm, to guide future studies and breeding of its ornamental use. A wide range of C. pepo germplasm was collected from diferent parts of the world. Nineteen variables were recorded in 20 varieties to determine the overall degree of polymorphism and to detect similarities among them. Qualitative traits of fruit such is shape, color and texture, showed immense variation. Coefficient of variation were highest for fruit length, fruit weight and number of fruits per plant (CV=56.69161.32%), while they were the lowest for leaf length (CV=20.65%). Morphological characterization is need to facilitate the use of C. pepo varieties in breeding work. Based on the PCA results, 20 accessions of squash are separate in five groups. Those groups are unique in qualitative and quantitative traits. Knowledge of genetic divergence among varieties is essential for breeding.


2021 ◽  
Vol 2 (5) ◽  
pp. 1-8
Author(s):  
Jannatul Ferdousi ◽  
Mohammad Zakaria ◽  
Md. Azizul Hoque ◽  
Satya Ranjan Saha ◽  
Nasrin Akter Ivy ◽  
...  

Sweet pepper is one of the most important nutritious vegetable and its demand is increasing day by day in Bangladesh indicating need to characterize and assess morphological variability for varietal improvement programme. Twenty-one sweet pepper genotypes from native and alien sources were characterized for twenty-six morphological traits using vegetative and reproductive appearances at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh from October 2018 to March 2019. Marked variation was exhibited among twenty-six qualitative traits (26) studied. Twenty-two (22) characters showed undeniable variation among the genotypes. The presence of higher percentage (61.90%) of light purple color at node were observed indicated high amount of anthocyanin content. Leaf shape is used as genotypes identifier at vegetative stage and herein three types of leaves were found with dark green color (76.19%) that is highly correlates with yield. In case of flower, same level of stigma exertion (61.90%) with 100% white color corolla indicates higher number of fruit sett were exhibited. Entire genotypes exerted one or more exclusive characters especially fruit shape and color in Bangladesh perspective which could be used as an important breeding tools. Fruit color was observed in various categories at intermediate and mature stage as for instance yellow, green, purple, orange, red etc. in addition higher percentage of blocky fruit shape (38.09%) were observed and these are the consumer fascinating attributes of sweet pepper. However, selection of genotypes with desirable morphological trait can be used for their exploitation of future breeding programme.


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