Isolation of Laurus nobilis Leaf Polyphenols: A Review on Current Techniques and Future Perspectives

In recent years, the market demand for products enhanced with ingredients derived from natural products, such as polyphenols, is rapidly increasing. Laurus nobilis L., known as bay, sweet bay, bay laurel, Roman laurel or daphne is an evergreen Mediterranean shrub whose leaves have traditionally been used in cuisines and folk medicine due to their beneficial health effects, which can nowadays be scientifically explained by various biological activities of the leaf extracts. Many of these activities can be attributed to phenolic compounds present in L. nobilis leaves which include flavonoids, phenolic acids, tannins (proanthocyanidins) and lignans. In order to enable efficient industrial utilization of these valuable compounds, it is crucial to establish optimal extraction procedures resulting in the highest yields and quality of the extracts. This paper offers the first systematic review of current literature on the influence of conventional and advanced extraction techniques, including microwave-assisted, ultrasound-assisted, enzyme-assisted, supercritical-CO2 and mechanochemical-assisted extraction on the phenolic content of L. nobilis leaf extracts, allowing more efficient planning of further research and simplifying the steps towards industrial utilization of this plant.

POSSIBILITIES OF INDUSTRIAL UTILIZATION OF FFF/FDM PROCESS FOR CHOSEN ELEMENT PRINTing

3D printing is a one of a new solution of manufacturing methods. This is caused by the ability of print a small part in a short time (rapid prototyping), but also because of the available materials. This is important because by using 3D printing, a specialized element is made for a specific apply. Compared with traditional production methods, such as turning and milling, 3D printing gives a wider possibility of making specific product patterns, thanks to which this method gained support and became a competitive one. Hence, the designing and manufacturing process based on it is more and more often referred as the “design-driven manufacturing”. This method gives the possibility of making personalized elements or a larger number of product variants, but also gives the possibility of manufacturing complete elements in one run. The article describes the possibilities of using 3D printing in the production of unusual elements in a uncertain situations.

Two novel cyanobacterial α-dioxygenases for the biosynthesis of fatty aldehydes

Abstractα-Dioxygenases (α-DOXs) are known as plant enzymes involved in the α-oxidation of fatty acids through which fatty aldehydes, with a high commercial value as flavor and fragrance compounds, are synthesized as products. Currently, little is known about α-DOXs from non-plant organisms. The phylogenic analysis reported here identified a substantial number of α-DOX enzymes across various taxa. Here, we report the functional characterization and Escherichia coli whole-cell application of two novel α-DOXs identified from cyanobacteria: CalDOX from Calothrix parietina and LepDOX from Leptolyngbya sp. The catalytic behavior of the recombinantly expressed CalDOX and LepDOX revealed that they are heme-dependent like plant α-DOXs but exhibit activities toward medium carbon fatty acids ranging from C10 to C14 unlike plant α-DOXs. The in-depth molecular investigation of cyanobacterial α-DOXs and their application in an E. coli whole system employed in this study is useful not only for the understanding of the molecular function of α-DOXs, but also for their industrial utilization in fatty aldehyde biosynthesis.Key points• Two novel α-dioxygenases from Cyanobacteria are reported• Both enzymes prefer medium-chain fatty acids• Both enzymes are useful for fatty aldehyde biosynthesis Graphical abstract

Tuber and Organoleptic Characteristics of Four Potato Varieties Grown Off-season in Sajang Village, Sembalun

Quality of the tuber is an important factor determining utilization and economic values of the potato (Solanum tuberosum L.) plant. The acceptance of potato variety for industrial processing is determined by the dry matter, starch and reduced sugar contents as well as market acceptance of the products. These characteristics are influenced by the potato variety and cultivation season. This research aimed to evaluate the tuber and organoleptic properties of chips from four potato varieties grown during off-season, in Sajang Village of Sembalun District (at ca. 900 m above mean sea level). A plantation was undertaken at the end of rainy season in 2021, and comprised of four potato varieties, designated as McRusset, Ranger Russet, Chitra and Atlantic. After harvesting and stored at ambient temperature for 2 weeks, raw tubers were characterized for the dry matter, starch, and reduced sugar contents. The tubers were fried as french fries and chips, and sensory analysis were undertaken for likeliness in color, flavour, crispness and overall appearance. The different in properties of these four varieties and its industrial utilization is be presented and discussed in this paper.

Diversifying Arena of Drug Synthesis: In the Realm of Lipase Mediated Waves of Biocatalysis

Hydrolases, being most prominent enzymes used in industrial processes have left no stone unturned in fascinating the pharmaceutical industry. Lipases, being a part of acyl hydrolases are the ones that function similarly to esterases (except an interfacial action) wherein they generally catalyze the hydrolysis of ester bonds. Be it in terms of stereoselectivity or regioselectivity, lipases have manifested their promiscuous proficiency in rendering biocatalytic drug synthesis and intermediates thereof. Industrial utilization of lipases is prevalent since decades ago, but their distinctive catalytic competencies have rendered them suitable for maneuverability in various tides of biocatalytic industrial process development. Numbers of exquisite catalysts have been fabricated out of lipases using nanobiotechnology whereby enzyme reusability and robustness have been conferred to many of the organic synthesis procedures. This marks a considerable achievement of lipases in the second wave of biocatalysis. Furthermore, in the third wave an advent of genetic engineering has fostered an era of customized lipases for suitable needs. Be it stability or an enhanced efficacy, genetic engineering techniques have ushered an avenue for biocatalytic development of drugs and drug intermediates through greener processes using lipases. Even in the forthcoming concept of co-modular catalytic systems, lipases may be the frontiers because of their astonishing capability to act along with other enzymes. The concept may render feasibility in the development of cascade reactions in organic synthesis. An upcoming wave demands fulfilling the vision of tailored lipase whilst a far-flung exploration needs to be unveiled for various research impediments in rendering lipase as a custom fit biocatalyst in pharmaceutical industry.

Production of Glucose Isomerase from Streptomyces roseiscleroticus

This study was aimed at the isolation and characterization of a microbial strain capable of producing glucose isomerase. Microbial strain was isolated from soil using starch casein agar as a differential media. The isolated microbial strain was capable of producing glucose isomerase which was tested using 2, 3, 5 - triphenyltetrazolium solution as a chromogenic substrate. The microbial strain was identified as Streptomyces species based on its morphological and microscopic characteristics. It was further subjected to molecular characterization using 16S rRNA sequencing and was subsequently confirmed as Streptomyces roseiscleroticus. Glucose isomerase was produced from Streptomyces roseiscleroticus after 120 hr of submerged fermentation at pH 6.8 and at 37°C utilizing xylose as the sole carbon source and a compendium of peptone, beef and yeast extracts as nitrogen sources. These findings suggest that the microbial strain, Streptomyces roseiscleroticus can be a useful bacterial source for the production of glucose isomerase needed for commercial and industrial utilization.

Generic Noninteger Order Controller for Time-Delay Systems

This paper focuses on the problem of fractional controller P I stabilization for a first-order time-delay systems. For this reason, we utilize the Hermite–Biehler and Pontryagin theorems to compute the complete set of the stabilizing P I λ parameters. The widespread industrial utilization of PID controllers and the potentiality of their noninteger order representation justify a timely interest in P I λ tuning techniques. Step responses are calculated through K p , K i , l a m b d a parameters inside and outside stability region to prove the method efficiency.

A Review: Photocatalysts Based on BiOCl and g-C3N4 for Water Purification

Many organic pollutants are discharged into the environment, which results in the frequent detection of organic pollutants in surface water and underground water. Some of the organic pollutants can stay for a long time in the environment due to their recalcitrance. Advanced oxidation processes (AOPs) can effectively treat the recalcitrant organic compounds in water. Photocatalysis as one of the AOPs has attracted a lot of interest. BiOCl and g-C3N4 are nice photocatalysts. However, their catalytic activity should be further improved for industrial utilization. The construction of heterojunction between the two different components is deemed as an efficient strategy for developing a highly efficient photocatalyst. As a typical type-II heterojunction, g-C3N4/BiOCl heterojunctions showed better photocatalytic performance. To date, the g-C3N4/BiOCl composites were mainly studied in the field of water purification. The photoactivity of the pristine catalysts was greatly enhanced by the combination of the two materials. However, three kinds of proposed mechanisms were used to explain the improvement of the g-C3N4/BiOCl heterojunctions. But few researchers tried to explain why there were three different scenarios employed to explain the charge transfer. According to the articles reviewed, no direct evidence could indicate whether the band structures of the heterojunctions based on BiOCl and g-C3N4 were changed. Therefore, many more studies are needed to reveal the truth. Having a clearer understanding of the mechanism is beneficial for researchers to construct more efficient photocatalysts. This article is trying to start a new direction of research to inspire more researchers to prepare highly effective photocatalysts.

Adaptive Laboratory Evolution of Microalgae: A Review of the Regulation of Growth, Stress Resistance, Metabolic Processes, and Biodegradation of Pollutants

Adaptive laboratory evolution (ALE) experiments are a serviceable method for the industrial utilization of the microalgae, which can improve the phenotype, performance, and stability of microalgae to obtain strains containing beneficial mutations. In this article, we reviewed the research into the microalgae ALE test and assessed the improvement of microalgae growth, tolerance, metabolism, and substrate utilization by ALE. In addition, the principles of ALE and the key factors of experimental design, as well as the issues and drawbacks of the microalgae ALE method were discussed. In general, improving the efficiency of ALE and verifying the stability of ALE resulting strains are the primary problems that need to be solved in future research, making it a promising method for the application of microalgae biotechnology.

Analysis and Performance Evaluation of Entropic Thresholding Image Processing Techniques for Electrical Capacitance Tomography Measurement System

To improve image quality generated from the electrical capacitance tomography measurement system, the use of entropic thresholding techniques is investigated in this article. Based on the analysis of the principle of Electrical Capacitance Tomography (ECT) image reconstruction and entropic thresholding, various algorithms have been proposed for easy extraction of quantitative information from tomograms generated from the ECT system. Experiments indicate that proposed algorithms can provide high-quality images at no or minimum computational cost. It is easier to implement and integrate with classical algorithms such as Linear Back Projection, Singular value decomposition, Tikhonov regularization, and Landweber. Entropic thresholding techniques present a feasible and effective way toward the industrial utilization of ECT measurement systems. Keywords: Electrical Capacitance Tomography; Inverse Problem; Image Reconstruction; Entropic Thresholding