scholarly journals Genetic Variability and Molecular Diversity Analysis in Turmeric (Curcuma longa L.)

2021 ◽  
Author(s):  
Priya Patel ◽  
R.K. Patel ◽  
K.G. Modha ◽  
Thokchom Joydeep Singh ◽  
Manju Singh
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Coefficient Of Variation ◽  
Molecular Diversity ◽  
Curcuma Longa ◽  
Block Design ◽  
Herbaceous Plant ◽  
Randomized Block Design ◽  
The Family ◽  
Agronomical Traits

Background: Turmeric is an age old herbaceous plant belonging to the family Zingiberaceae growing in Tropical Africa and India with huge national and international demands. Conventionally, turmeric accessions are characterized using morphological and agronomical traits but less work is done in molecular characterization which is needed for thorough trait identification. Methods: The investigation was carried out during Kharif 2016-17 and comprised of thirty diverse genotypes of turmeric analyzed for genetic variability and molecular diversity which were evaluated in randomized block design with two replications. Fresh tender leaves were used to isolate DNA and PCR was performed with 9 SSR markers. Result: The values of phenotypic coefficient of variation were slightly higher than genotypic coefficient of variation. Highest heritability was observed for days to maturity, genetic advance was observed highest for green rhizome yield. Out of 9 SSR markers, primer pair 5, 6, 7, 8 and 9 were reported to exhibit 100% polymorphism, whereas; in terms of PIC, primer pairs 6 and 9 were found to be highly efficient ones. The similarity coefficient ranged from 0.44 to 1.00 and dendogram categorized the 30 genotypes into two main clusters.

Genetic variability of Indonesian landraces of Vigna subterranea: Morphological characteristics and molecular analysis using SSR markers

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
MUHAMMAD FAUZAN FARID ALHAMDI ◽  
Asep Setiawan ◽  
Satriyas Ilyas ◽  
Wai Kuan Ho
Keyword(s):  
Genetic Variability ◽  
Ssr Markers ◽  
Molecular Analysis ◽  
Block Design ◽  
Leaf Shape ◽  
Morphological Characteristics ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Alternative Source ◽  
Randomized Block Design

Abstract. Alhamdi MFF, Setiawan A, Ilyas S, Ho WK. 2020. Genetic variability of Indonesian landraces of Vigna subterranea: The morphological characteristics and molecular analysis using SSR markers. Biodiversitas 21: 3929-3937. Bambara groundnut (Vigna subterranea (L.) Verdc.) is a potential grain, which can be considered as an alternative source of protein and carbohydrate. Due to unavailability of commercial bambara groundut cultivar in Indonesia, the characterization of bambara groundnut landraces is an important step before developing cultivar with traits of interest. The objective of the research was to access genetic variability of Indonesian landraces of bambara groundnut with different seed coat colors based on morphological and molecular markers. The experiment was arranged as split-plot in a complete randomized block design with the main plot was cultivation methods and the sub plot was landraces. There were differences in leaf shape and pod shape among the landraces. There were two main clusters of Indonesian landraces of bambara groundnut with 88.28% similarity. The first cluster was Cream, Brown Sumedang, Black Sumedang and Black Tasikmalaya, and the second cluster was Black Sukabumi, Brown Gresik, Black Madura, and Black Gresik. The result based on SSR marker with capillary electrophoresis indicated Black Gresik and Black Madura landraces were different from other Indonesian landraces.  Cream Sumedang or Brown Sumedang from the first cluster and Black Gresik or Brown Gresik from the second cluster have the farthest distances for developing improved variety of bambara groundnut.

scholarly journals Genetic variability and agronomic performance studies in chilli (Capsicum annum L.)

2017 ◽  
Vol 14 (2) ◽  
pp. 56-62
Author(s):  
D Kannan ◽  
DK Singh ◽  
SK Singh ◽  
A Palanisamy
Keyword(s):  
Genetic Variability ◽  
Performance Studies ◽  
Coefficient Of Variation ◽  
Block Design ◽  
Stem Diameter ◽  
Agronomic Performance ◽  
Genetic Advance ◽  
Plant Weight ◽  
Capsicum Annum ◽  
Randomized Block Design

Eight diverse genotypes of chilli were evaluated an open field study to evaluate the genetic variability, heritability and genetic advance in randomized block design (RBD) with three replications during Kharif, 2015-16. Analysis of variance revealed significant differences among the genotypes for all the characters studied. The higher estimates of genotypic coefficient of variation (GCV) were observed for flowers per branch (21.59%), clusters per plant (19.26%), flower per branch (16.93%) and stem diameter (15.49%). While the higher estimates of phenotypic coefficient of variation (PCV) were found for flowers per branch (26.70%), fruits per branch (24.44%), clusters per plant (24.04%) and stem diameter (19.26%). The higher estimates of broad sense heritability along with genetic advance recorded for flowers per branch (65%), fruits per plant (64%), cluster per plant (64%), stem diameter (65%), plant weight (59%) and days to 50% flowering (50%) indicated the scope for improvement of these characters through selection.SAARC J. Agri., 14(2): 56-62 (2016)

scholarly journals Assessment of Genetic Variability and Transmissibility Index in Rice Genotypes (Oryza sativa L.)

2021 ◽  
pp. 34-42
Author(s):  
Ashish Sheera ◽  
Nashra Aftab ◽  
Sandeep Rout ◽  
Udit Nandan Mishra ◽  
Bupesh Sharma ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Grain Yield ◽  
Coefficient Of Variation ◽  
Oryza Sativa L ◽  
Block Design ◽  
Uttar Pradesh ◽  
Grain Filling ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Days To Maturity

Aim: To assess the Genetic Variability among the accession/ genotype and its contributing traits. Methodology: A field experiment was conducted using 35 genotypes including 2 checks of rice during the season of kharif, 2017, at the Field Experimental Centre, Department of Genetics and Plant Breeding, SHUATS, Prayagraj, Uttar Pradesh to evaluate genetic variation and heritability of yield and related traits. The experiment was designed with a randomized block design (RBD) with three replications. Analysis of variance revealed significant difference among the genotypes for all traits indicating existence of genetic variability among the accessions. Results: Highest genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were recorded for Spikelet’s per panicle, tillers per hill, panicle per hill and grain filling. High heritability in broad sense was obtained for days to 50% flowering (99.6%), days to maturity (98.7%), test weight (97.9%), harvest index (96.9%) and grain yield per hill (96.6%) which is indicating the high heritable portion of variation. High to medium estimate of heritability in genetic advance were obtained for grain yield (38.51), spikelet’s/ panicle (37.94), panicle/ hill (36.97) were indicating the roles of additive gene action and good scope of selection using their phenotype performance. Interpretation: Considering, all of these characters, spikelet’s per panicle, days to 50% flowering and days to maturity were important yield related traits and could be used for selection in future rice breeding programme.

scholarly journals Genetic variability, heritability and genetic advance studies in Amaranthus (Amaranthus tricolor L.)

2021 ◽  
pp. 77-81
Author(s):  
SANJAY KUMAR
Keyword(s):  
Genetic Variability ◽  
Coefficient Of Variation ◽  
Seed Weight ◽  
Block Design ◽  
Test Weight ◽  
Genetic Advance ◽  
Amaranthus Tricolor ◽  
Randomized Block Design ◽  
Significant Difference ◽  
Full Maturity

The experiment was conducted during 2016-17 at Horticulture Research Farm, Department of Applied Plant Science (Horticulture), Babasaheb Bhimrao Ambedkar University, Lucknow to study the “genetic variability, heritability and genetic advance in Amaranthus(Amaranthus tricolor L.)”.The experiment was laid out in randomized block design with three replications. The analysis of variance revealed significant difference among all the genotypes for the attributes. Higher genotype coefficient of variation (GCV) was observed for seed weight per plot, spikes per plant, leaves per plant and branches per plant. Thephenotype coefficient of variation(PCV) was also found higher in leaves per plant followed by branch, spike per plant and seed weight per plant. Maximum heritability (broad sense) was obtained for seed weight per plot followed by test weight, green leaves yield per plant and days to full maturity. The genetic advance was found maximum for length of leaves followed by test weight, days to full maturity and percent dry matter.

scholarly journals Genetic variability studies in germplasm of wheat (Triticum aestivum L.)

2021 ◽  
Vol 23 (3) ◽  
pp. 310-313
Author(s):  
NISHANT PATIAL ◽  
Keyword(s):  
Genetic Variability ◽  
Grain Yield ◽  
Leaf Area ◽  
Coefficient Of Variation ◽  
Flag Leaf ◽  
Block Design ◽  
Triticum Aestivum L ◽  
Randomized Block Design ◽  
Yield Test ◽  
Breeding Programmes

In the present investigation, twenty genotypes of bread wheat (Triticuma estivum L.) were evaluated to examine the presence of genetic variability which may further be utilized in breeding programmes. The experiment was laid out in randomized block design with three replications at the experimental field of Himgiri Zee University, Dehradun during 2019 - 2020. Analysis of variance revealed that genotypes possessed significant genetic variability among all traits.It was observed that HUW-251 (recorded the highest value 67.33 g) where as HUW-213 exhibited the lowest value (48.00 g) for grain yield per plant.The highest estimates of genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were observed for spike weight(g) (19.97, 19.98) respectively, followed by flag leaf area (15.64) and spikelets per spike (14.49, 14.59).High heritability (h2) associated with high genetic advance as percent of mean (GAM) were observed for grain yield, test weight, grains per spike, spikelets per spike, spike weight, spike length, peduncle length and flag leaf area.

Assessment of genetic variability for quantitative characters in rice (Oryza sativa L.) under post flood situation in sali

2021 ◽  
Vol 58 (1) ◽  
pp. 43-48
Author(s):  
Dalibha Pathak ◽  
Umesh Ch. Kalita
Keyword(s):  
Grain Yield ◽  
Coefficient Of Variation ◽  
Quantitative Traits ◽  
Oryza Sativa L ◽  
Block Design ◽  
Genetic Advance ◽  
Spikelet Sterility ◽  
Rice Varieties ◽  
Randomized Block Design ◽  
Spikelets Per Panicle

Sixteen genotypes of rice (including one check) were evaluated on 18 quantitative traits during sali (August), 2018 in a randomized block design with three replications at Instruction cum Research Farm, Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat to study the nature and magnitude of variability, heritability and genetic advance under delayed sown condition. The analysis of variance for 18 quantitative traits revealed the presence of significant differences for grain yield and its component characters. The highest genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) was observed for chaffs per panicle followed by grains per panicle, spikelet sterility (%), spikelets per panicle, grain yield (kg/ha). A high heritability coupled with high genetic advance was observed for plant height (cm), spikelets per panicle, spikelet sterility (%), chaffs per panicle, grains per panicle, grain yield (g/hill), grain yield (kg/ha), biological yield (kg/ha), straw weight (kg/ha) and harvest index (%) indicating that selection might be effective for improvement of these characters under delayed sown condition with low input. From the findings of this investigation, one genotype viz., Gandhari emerged as the outstanding genotype which yielded 4170 kg/ha and could be directly used for cultivation in delayed sown situation with low inputs. Some other promising genotypes that yielded higher than the check Manoharsali under delayed sown condition were JR 29, JR 16, Basundhara and JR 60 and could be utilized as potential parental material in the hybridization programmes designed to develop suitable rice varieties for delayed sown situation.

scholarly journals Culinary and nutritional quality of common bean lines with Carioca grain type and interaction with environments

Revista CERES ◽  
2017 ◽  
Vol 64 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Helton Santos Pereira ◽  
Renata Cristina Alvares ◽  
Leonardo Cunha Melo ◽  
Antônio Félix da Costa ◽  
Hélio Wilson Lemos de Carvalho
Keyword(s):  
Genetic Variability ◽  
Common Bean ◽  
Protein Content ◽  
Block Design ◽  
Genetic Effect ◽  
Cooking Time ◽  
High Protein ◽  
High Protein Content ◽  
Randomized Block Design ◽  
Distrito Federal

ABSTRACT The objectives of this work were to study the genetic variability and the interaction between genotypes and environments for cooking time and protein content of bean grains as well as to identify elite lines of Carioca grain type with short cooking time, high protein content and high adaptability and stability for these two traits. Sixteen experiments were conducted in a complete randomized block design with three replications during the rainy, dry and winter seasons, in Goiás, Distrito Federal, Pernambuco, Sergipe, Bahia and Paraná States, in 2009 and 2010. Each trial was composed by 16 elite lines of Carioca grain type and the data of cooking time and protein content were obtained. Data were submitted to analysis of variance and to stability and adaptability analysis, according to the methodology proposed by Annichiarico. Genetic variability was found for cooking time and for protein content among Carioca common bean elite lines; however, for protein content this variability is lower. The environmental effect is important for the expression of these traits and is larger than the genetic effect. The interaction between genotypes and environments is important for cooking time and for protein content of common beans. The lines CNFC 11951 and CNFC 11962 presents short cooking time, high protein content and high stability and adaptability for both traits.

scholarly journals REACTION OF BANANA CULTIVARS TO THE Meloidogyne javanica X Fusarium oxysporum f. sp. cubense COMPLEX

2018 ◽  
Vol 31 (3) ◽  
pp. 572-583
Author(s):  
LEANDRO DE SOUZA ROCHA ◽  
ROBERT FELIX DE SANTANA ◽  
ANA CRISTINA FERMINO SOARES ◽  
FERNANDO HADDAD
Keyword(s):  
Genetic Variability ◽  
Fusarium Oxysporum ◽  
Block Design ◽  
Meloidogyne Javanica ◽  
Simultaneous Presence ◽  
Sterile Soil ◽  
Panama Disease ◽  
Randomized Block Design ◽  
Resistant Varieties ◽  
Inoculation Time

ABSTRACT The interaction Fusarium oxysporum f. sp. cubense (Foc) x nematode and the genetic variability of Foc are the main problems with potential to affect the use of resistant varieties in the management of the Panama disease. The objective of this work was to evaluate the interaction between Foc and Meloidogyne javanica on the banana of the Prata Anã, Grande Naine and BRS Princesa cultivars. The experiment was conducted in a randomized block design, with six treatments and ten replications, which differed in the inoculation time of each pathogen. Simultaneous inoculation with Foc and M. javanica, inoculation with Foc one week before inoculation with M. javanica, and inoculation with M. javanica one week before inoculation with Foc. In addition to the controls, Foc isolated, M. javanica isolated, and cultivars without the pathogens. The seedlings were transplanted in 3-liter pots with sterile soil infested with 40 grams of Foc inoculum at the concentration of 1x106CFU/gram and 1000 eggs and J2 of M. javanica. At the end of 56 days, the treatments with 'Grande Naine' presented the highest reproduction factors of M. javanica. In the 'Prata Anã' no interaction was observed between Foc x M. javanica. The treatments with simultaneous inoculation of the two pathogens, and with Foc a week before, in the 'BRS Princesa', presented the highest external disease indices (DI), which promoted the highest AUDPC. The highest internal DI were observed in the treatments Foc+Mj+Grande Naine, Foc+Mj+BRS Princesa, Mj+Grande Naine+Foc and Foc+BRS Princesa+Mj. The simultaneous presence of M. javanica and Foc increases the severity of the Panama disease in Grande Naine and BRS Princesa cultivars.

scholarly journals Seleção de genótipos de batata-doce quanto à eficiência ao uso do fósforo em solos da região de cerrado

2013 ◽  
Vol 4 (4) ◽  
pp. 356-364
Author(s):  
Luziano Lopes Da Silva ◽  
Márcio Antônio Da Silveira ◽  
Rodrigo Ribeiro Fidelis ◽  
Rodrigo de Castro Tavares ◽  
Valéria Gomes Momenté ◽  
...  
Keyword(s):  
Experimental Design ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Tuberous Root ◽  
Phosphorus Fertilization ◽  
Germplasm Bank ◽  
Randomized Block Design ◽  
The Family ◽  
Selection Of

The sweet potato (Ipomoea batatas (l.) Lam.) Lam) is a plant that produces tuberous root belonging to the family convolvulácea explored in practically all states of Brazil. The objective of this work was to select genotypes of the sweet potato as the efficiency of phosphorus use in cerrado soils with high and low availability of this nutrient. Were evaluated nine genotypes of sweet potato coming from the germplasm bank from the Federal University of the Tocantins, Brazil. The experimental design was in randomized block design with three replications being nine genotypes grown in two environments with low and high phosphorus availability, using doses of phosphorus fertilization from 20 and 120 kg ha-1 P2O5, applied at planting. To select genotypes suitable for environments proposed was used methodology for selection of the efficient use and response to phosphorus fertilization (efficiency and response). Genotype Amanda was classified as efficient and responsive, Barbara, Julia, Marcela and Carolina Vitoria as efficient, but not responsive and Livia, Duda, Ana Clara and Beatriz were classified as non-efficient, responsive but the absorption and utilization of phosphorus.

scholarly journals Influence of off-season land management on maximizing yield and quality of Curcuma longa L. cultivars

1970 ◽  
pp. 33-36
Author(s):  
A. ANBURANI
Keyword(s):  
Land Management ◽  
Management Practices ◽  
Curcuma Longa ◽  
Block Design ◽  
Quality Parameters ◽  
Polyethylene Film ◽  
Superior Performance ◽  
Yield And Quality ◽  
Randomized Block Design

The present investigation was carried out to study the effect of off season soil management practices on yield and quality of turmeric (Curcuma longa L.) cultivars. The experiment was laid out in a Factorial Randomized Block Design with ten treatments in three replications consisted of five off-season land management treatments viz., fallow (S1), summer ploughing 2 times (S2), summer ploughing 1 time (S3), solarization with transparent polyethylene film of 0.05 mm thick for 40 days (S4) and black polyethylene film for 40 days (S5). It was tested with two popular cultivars viz., Curcuma longa -1 CL-1 (V1) and Curcuma longa-2 CL-2 (V2), collected from Erode and Chidambaram. Various yield components were recorded at the time of harvest and were analysed. The yield attributing characters viz., number, length, girth and weight of mother, primary and secondary rhizomes were recorded. The treatment where solarization with transparent polyethylene film of 0.05 mm thick was tested recorded the highest yield and yield attributing characters when compared to other treatments. The same treatment also exhibited the highest fresh rhizome yield per plant, curing percentage and cured rhizome yield. The quality parameters like curcumin, oleoresin and essential oil content were also showed superior performance in the treatment where solarization with transparent polyethylene film of 0.05 mm thick was applied.

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