scholarly journals Potential of Moringa (Moringa oleifera) leaf extract to inhibit the growth of pathogenic bacteria Edwardsiella tarda

2021 ◽  
Vol 10 (3) ◽  
pp. 321
Author(s):  
Farid Mukhtar Riyadi ◽  
Arief Prajitno ◽  
Mohamad Fadjar ◽  
Arif Syaifurrisal ◽  
Annisa Isti Fauziyyah
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Positive Control ◽  
Edwardsiella Tarda ◽  
Diffusion Method ◽  
Tryptone Soya Agar ◽  
Agar Media ◽  
Moringa Leaf Extract

This study analyzed the antibacterial activity of Moringa oliefera leaf extract against the growth of Edwardsiella tarda bacteria. This study aims to determine the antibacterial activity of Moringa leaf extract (M. oliefera) against the growth of E.tarda bacteria. Inhibition testing is done by diffusion method (disc test). The disc test used five variations of concentration of 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L, and 375 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. As a positive control, an antibiotic in the form of chloramphenicol was used. (5 mg/L) Moreover, distilled water was used as a negative control.Moringa leaf extract (M. oliefera) contains natural active compounds, bacteriostatic antibacterials, due to decreased bacterial growth after 48 hours of incubation. The highest inhibition diameter of E. tarda was 12.95 mm at a concentration of 375 mg/L after 24 hours incubation and decreased by 11.02 mm after 48 hours incubation. The highest inhibitory effectiveness was at a concentration of 375 mg/L with an effectiveness of 58.8%, while the effectiveness of the decrease was 48.1% after 48 hours of incubation.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Green Synthesis of Silver Nanoparticles Using Moringa Oleifera Leaf Extract, Tamarindus Indica Fruit Extract and Assessement of Antibacterial Activity Against Staphylococcus Aureus

2021 ◽  
Author(s):  
Shirisha A ◽  
ANUMOLU VIJAYA KUMAR ◽  
Laxman Chatlod R ◽  
Shashi Kumar M ◽  
Krishnaiah N ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Diffusion Method ◽  
Tamarindus Indica ◽  
Fruit Extract

Abstract The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this study for synthesis of silver nanoparticles different ratios of 1mM silver nitrate and Moringa oleifera leaf extract i.e, 95:5, 90:10 and 85: 15 was taken in conical flask and kept for one 1 hr at 25 0 c on magnetic stirrer, out of which 90:10 ratio was selected for further study based on highest peak, good size and stability. Tamarindus indica fruit extract was added to silver nitrate solution till the colour of the solution changes from light brown to chocolate brownish colour. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy, Zeta potential, size distribution by intensity. The absorption spectrum of the silver nano solution prepared by using Moringa oleifera and Tamarindus indica fruit extract showed a surface plasmon absorption band with maximum of 420 nm and 430 nm respectively indicating the presence of silver nanoparticles. The zeta value of silver nanoparticles synthesized from Moringa oleifera and Tamarindus indica fruit extract was -12.5 mV and -15.5 mV, size of 110.2 nm and 130.2 nm respectively. The antibacterial efficacy of nanosilver was checked by agar well diffusion method, and the silver nanoparticles showed effective antibacterial activity against Staphylococcus aureus.

scholarly journals Antibacterial Activity and Cytotoxicity of Three Lectins Purified from Drumstick (Moringa oleifera Lam.) Leaves

1970 ◽  
Vol 17 ◽  
pp. 89-94 ◽  
Author(s):  
Shahanaz Khatun ◽  
MMH Khan ◽  
M Ashraduzzaman ◽  
Farzana Pervin ◽  
Luthfunnessa Bari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Brine Shrimp ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Antibacterial Activities ◽  
Artemia Salina ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Plant Materials ◽  
Gram Negative

Context: Plant materials contain glycoproteins (phytolectins) that are toxic in nature may play a key role in the control of various normal and pathological processes in living organisms and have diverse biochemical and diagnostic applications. Objectives: Screening of three lectins SLL-1, SLL-2 and SLL-3 purified from Drumstick (Moringa oleifera Lam.) leaves for their antibacterial activities and brine shrimp lethality bioassay. Materials and Methods: Three bioactive lectins were purified from Drumstick leaves by conventional chromatographic methods. The lectins were tested for their antibacterial activities against three pathogenic bacteria- Escherichia coli (gram-negative) Shigella dysenteriae (gram-negative) and Staphylococcus aureus (gram-positive) using the standard disc-diffusion method. Mortality of the brine shrimp naupli was assessed by hality bioassay. Results: All the lectins showed antibacterial activity against E. coli, Sh. dysenteriae and St. aureus. They also showed cytotoxic effect in brine shrimp (Artemia salina L.) lethality bioassay. The LC50 values of SLL-1, SLL-2 and SLL-3 were found to be 15.8, 17.78 and 14.12 μg/ml respectively. The experimental results revealed that SLL-3 is more cytotoxic than other lectins. The lectin SLL-3 showed lowest activity whereas SLL-1 showed highest activity against the three bacteria. Conclusion: Results suggest that the extracts from M. oleifera leaf can be a source of natural antimicrobials with potential applications in pharmaceutical industry to control coliform bacteria.Key words:  Drumstick; Moringa oleifera; lectins; antibacterial activity; brine shrimp; bioassayDOI: 10.3329/jbs.v17i0.7112J. bio-sci. 17: 89-94, 2009

scholarly journals GEL ANTI JERAWAT EKSTRAK DAUN BUTA-BUTA (Excoecaria agallocha L.) DAN PENGUJIAN ANTIBAKTERI Staphylococcus epidermidis

2015 ◽  
Vol 1 (2) ◽  
pp. 65-72
Author(s):  
Ika Olivia Borman ◽  
Yusriadi Yusriadi ◽  
Evi Sulastri
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Diffusion Method ◽  
Activity Data ◽  
Excoecaria Agallocha ◽  
Significant Difference ◽  
The Stability

Buta-Buta (Excoecaria agallocha L.) leaf is a type of mangrove plants that has antibacterial activity againtsbacteriacausing acne, Staphylococcus epidermidis,. This study aims to formulate buta-buta leaf extract with the concentration of 12.5 % into anti-acne gel using two gels, namely HPMC 8% (Formula 1) and Carbopol 940 2%(Formula 2). Antibacterial activity test was carried out by agar diffusion method using well. The stability and antibacterial activity data during 28 days were analyzed statistically using unpaired t-student with a degree of confidence of 95% (α = 0.05). The evaluation results of anti-acne gel form of buta-buta leaf extract in Formula 2 showedbetter characteristics than Formula 1, which include organoleptic examination, homogenity, pH and viscosity during 28 days. The antibacterial test showed the diameter of inhibition zone of Formula 2 is 11.03 mm ±0,01. The statistic analysis showed a significant difference between Formula 2 with a positive control (klindamisin gel).

scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella thypi

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 268
Author(s):  
Serly D. S. Toding ◽  
Herny E. I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Inhibitory Power ◽  
Paper Disc

ABSTRACT One of the potential medicinal plants as traditional medicine is gardenia plant (Gardenia augusta). Gardenia leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and triterpenoids, which are known to have antibacterial abilities..The aim of this research was to determine the antibacterial activity of gardenia leaf extract on the growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi. The examination on the inhibitory power using the Kirby-Bauer method. The Kirby-Bauer method is referring to the diffusion method using paper disc with five treatments, namely the extract of gardenia leaves with a substance concentration of 20%, 40%, and 60% as well positive control (Ciprofloxacin) and negative control (aquades). Based on the research results obtained, it can be concluded that gardenia leaf extract can inhibit growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi at a concentration of 20%, 40% and 60% and classified as strong and medium category. Keywords  :    Gardenia augusta, antibacterial, of Staphylococcus aureus, Escherichia coli, Salmonella thypiABSTRAK  Salah satu tanaman obat dengan potensi sebagai obat tradisional adalah tanaman kacapiring (Garddenia augusta). Daun kacapiring mengandung senyawa alkaloid, flavonoid, tanin, saponin dan triterpenoid, yang berpotensi sebagai antibakteri sehingga digunakan dalam penelitian ini.Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kacapiring terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Salmonella thypi. Pengujian daya hambat menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas dengan 5 perlakuan yaitu ekstrak daun kacapiring dengan konsentrasi 20%, 40%, dan 60% serta kontrol positif (ciprofloxacin) dan kontrol negatif (akuades). Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak daun kacapiring dapat menghambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella thypi pada konsentrasi 20%, 40% dan 60% dan tergolong dalam kategori kuat dan sedang. Kata Kunci  : Kacapiring, Antibakteri, Staphylococcus aureus, Escherichia coli, Salmonella thypi

scholarly journals Antimicrobial activities and phytochemical screening of leaf extract of Mikania micrantha H.B.K

2018 ◽  
Vol 30 ◽  
pp. 274-286
Author(s):  
Anjana Devkota ◽  
Anita Sahu
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Mikania Micrantha ◽  
Crude Leaf Extract

Antimicrobial activities and phytochemical screening of leaves of Mikania micrantha was tested in laboratory against phytopathogenic fungi and human pathogenic bacteria. The leaves samples were extracted in distilled water and methanol. The crude extracts of leaves were assessed in-vitro for antimicrobial activity using different concentrations (50, 100, 150,200, and 250 mg/ml) against five fungal strains (viz. Sclerotium rolfsii, Phytopthora capsici, Alternaria brassicae, Fusarium oxysporum and Botrytis cinerea) and six bacterial strains (Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, Bacillus subtilis, Enterococcusfaecalis and Staphylococcus aureus). Antifungal activity was tested by Poison Food Technique and Linear Mycelium growth Reduction (LMGR) percentage was determined. In crude extractof M. micrantha, the highest LMGR percentage was found in F. oxysporum in both distilledwater and methanol extract. Antibacterial activity was carried out by Disc Diffusion method. In the crude leaf extract of M. micrantha Gram negative bacteria was found more resistant than Gram positive bacteria. Methanol extract was found more effective in determining the Zone of Inhibition for all the strains of bacteria in all the concentrations. The plant extracts were found more effective in showing antibacterial activity than antifungal activity. The phytochemicalscreening revealed that the selected species contained tannin, saponins, alkaloid, flavonoid, cardiac glycosides and terpenoids. This result supports the potential of this plant species used as a new chemotherapeutic drug.

Antibacterial Activity and Phytochemical Screening of Erythrina fusca Lour. Leaf Extract (Fabaceae)

2022 ◽  
Vol 16 (1) ◽  
pp. 60
Author(s):  
Adiez Sapura Azmi ◽  
Mohammad Humayoon Amini ◽  
Muhammad Farhan Syakir Nor Azman ◽  
Fatimah Salim ◽  
Ali Jawad
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Positive Control ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Phytochemical Screening ◽  
Antibacterial Screening

Erythrina fusca Lour. (family Fabaceae) is a flowering tree that is locally known as ‘chengkering’. The plant is traditionally used in treatment of some symptoms related to bacterial infections such as wound infections, inflammations, and skin itching. This work reports for the first time in vitro antibacterial screening of the E. fusca Lour. methanolic leaf extract against some common Gram-negative and Gram-positive bacteria such as Klebsiella pneumonia, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, and Staphylococcus aureus. The in vitro antibacterial assay was carried out using agar   diffusion method with the extract concentration of 3 mg/well and vancomycin 30 µg/well as the positive control. It was found that the extract exhibits antibacterial effects in range of 10.5 – 14 mm of zone of inhibition (ZOI) against all tested bacteria except for K. pneumoniae. P. aeruginosa showing ZOI of 14 mm was the most sensitive bacterium while P. vulgaris (ZOI of 10.5 mm) was the least sensitive strain to the extract. Based on the preliminary phytochemical screening of using standard qualitative phytochemical tests, this species contains significant amount of alkaloids, flavonoids, terpenoids, and tannins, which might contribute to the antibacterial activity of the extract. Both antibacterial potential and presence of various phytochemicals in the extract could support the traditional uses of E. fusca  , and the obtained results would serve as a basis for further exploration of antibacterial properties of the plant’s leaves.

scholarly journals Test for the antibacterial inhibition of kaffir lime leaf (Citrus hysteric D.C) extract against pathogen bacteria in improving food safety

2021 ◽  
Vol 883 (1) ◽  
pp. 012056
Author(s):  
S J Nendissa ◽  
D M Nendissa
Keyword(s):  
Antibacterial Activity ◽  
Food Safety ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Abstract Kafir lime leaf (Citrus hystrix) is a plant from the citrus tribe that has long been known by community as flavor ingredient. To support its use and increase its application in supporting food safety, a test the inhibition of on kaffir lime leaf extract against pathogenic bacteria, namely Gram Negative Bacteria (Escherichia coli, Salmonella typhimurium) and Gram Positive bacteria (Staphylococcus aereus, P. aeroginosa). Making kaffir lime leaf extract (Citrus hystrix) was done by weighing 150g of lime leaf powder, then immersing in 96% ethanol solution and leaving for + 3 days. Kaffir lime leaf extract was dissolved with sterile distilled aquades to obtain a concentration of 5%, 10% and 15%. The antibacterial activity of kaffir lime leaf extract was tested by diffusion method using disc paper to determine of the bacterial growth inhibition area. The results showed that kaffir lime extract had antibacterial activity inhibition of 12,78 mm of S. aereus, 9 mm of E.coli, 7,12 mm of S. typhimurium and 9,3 mm of P. aeroginosa. Kaffir lime leaf extract has inhibition effectiveness for gram positive bacteria Staphylococcus aereus and gram negative bacteria E. coli, Salmonella typhimurium, P. aeroginosa. Thus, kaffir lime leaf extract can be used as a decontaminant agait theses 4 type of bacteria, especially Staphylococcus aereus which has a strong inhibitory power, so it can maintain quality and increase the safety of mead based foods

scholarly journals In Vitro Analysis of Antibacterial Activities of Curry Leaf (Murraya koenigii) Extract Towards Bacteria Edwardsiella tarda

2021 ◽  
Vol 10 (2) ◽  
pp. 221
Author(s):  
Arif Syaifurrisal ◽  
Arief Prajitno ◽  
Mohamad Fadjar ◽  
Farid Mukhtar Riyadi ◽  
Annisa Isti Fauziyyah
Keyword(s):  
Leaf Extract ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Test Methods ◽  
Negative Control ◽  
Edwardsiella Tarda ◽  
Murraya Koenigii ◽  
Tryptone Soya Agar ◽  
Vitro Analysis

This study analyzed the antibacterial activity of curry leaf extract (Murraya koenigii) on the growth of Edwardsiella tarda bacteria. This study aims to determine the bioactivity and antibacterial effectiveness of M. koenigii leaf extract against the growth of E.tarda bacteria. Inhibition test was carried out by delusion (MIC test) and diffusion (disc test) methods. MIC test used 5 variations of concentration: 1 mg/L, 10 mg/L, 100 mg/L, 500 mg/L and 1,000 mg/L on TSB (Tryptone Soya Broth) media; it was incubated for 24 hours. While the disc test used 5 variations of concentration: 100 mg/L, 200 mg/L, 300 mg/L, 400 mg/L and 500 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. Chloramphenicol (5 mg/L)  was used as a positive control, and distilled water was used as a negative control.                    M. koenigii leaf extract contains natural bioactive; it was bacteriostatic antibacterial due to bacteria's growth after 48 hours incubation. The highest inhibition diameter of E.tarda was 7,20 mm at a concentration of 500 mg/L after 24 hours incubation. The highest inhibitory effectiveness was at a concentration of 500 mg/L with effectivity 56.3%, while it declined to 46,44% after 48 hours incubation.

scholarly journals ANTIBACTERIAL ACTIVITY OF KARAMUNTING (RHODOMYRTUS TOMENTOSA (AITON) HASSK) LEAF EXTRACT AND FRACTIONS

2018 ◽  
Vol 11 (3) ◽  
pp. 163
Author(s):  
Siska Esperanza Sinulingga ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Dwi Suryanto
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Rhodomyrtus Tomentosa ◽  
Agar Well Diffusion Method

 Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa infection.Methods: Phytochemical screening is done in the extract and fractions of karamunting leaf continue with Standard agar well diffusion method for S. aureus and P. aeruginosa. Dimethyl sulfoxide was used as negative control, and standard antibiotic Kalmicetine (chloramphenicol) was used as positive control. Result: The ethyl acetate fraction showed the highest antibacterial activity against S. aureus and P. aeruginosa. Conclusion: Karamunting leaf can be used to treat the bacterial infection diseases especially infection from S. aureus and P. aeruginosa.

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