Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.

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SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov

Agric ◽

10.24246/agric.2015.v27.i1.p30-37 ◽

2016 ◽

Vol 27 (1) ◽

pp. 30

Author(s):

Elizabeth B.E Kristiani ◽

Sri Kasmiyati ◽

Maria Marina Herawati

Keyword(s):

Antibacterial Activity ◽

Petroleum Ether ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Phytochemical Analysis ◽

E Coli ◽

Randomized Design ◽

Antibactrial Activity

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.

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25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v13i2.3654 ◽

2019 ◽

Vol 13 (2) ◽

Author(s):

Silvia P.N Keliat ◽

Darniati Darniati ◽

Abdul Harris ◽

Erina Erina ◽

Rinidar Rinidar ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Average Diameter ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Final Conclusion ◽

Distilled Water

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.

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UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN SIRSAK (ANNONA MURICATA LINN.) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES

BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) ◽

10.31289/biolink.v6i1.2244 ◽

2019 ◽

Vol 6 (1) ◽

pp. 65

Author(s):

Yurlina Zai ◽

Agnes Yohana Kristino ◽

Sri Lestari Ramadhani Nasution ◽

Oliviti Natali

Keyword(s):

Leaf Extract ◽

Propionibacterium Acnes ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Annona Muricata ◽

Research Model ◽

Negative Controls

This research aims to determine the effectiveness of the antibacterial soursop leaf extract (Annona muricata L.). The method used is the agar diffusion method. The leaves were extracted by maceration method, using ethanol as a solvent. The soursop leaf extract obtained was then diluted using ethanol 96% at a concentration of 20%, 40%,60%, 80%. Positive control using clindamycin and negative controls using sterile aquadest. The research model used in this study is an experimental research model In Vitro of type. The results showed that soursop leaf extract had antibacterial effectiveness against Propionibacterium acnes with an average inhibition zone diameter of 9,7 mm; 13,7 mm; 15,7 mm; 16,3 mm; positive control (clindamysin) 21,0 mm; and negative control (aquadest) have no value tobe able to inhibit bacterial growth.

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Potensi Bacillus licheniformis dan Streptomyces olivaceoviridis sebagai Penghambat Pertumbuhan Jamur Saprolegnia sp. Penyebab Saprolegniasis pada Ikan secara In Vitro [Potential of Bacillus licheniformis and Streptomyces olivaceoviridis as Inhibiting The Growth of Fungus Saprolegnia sp, Cause Saprolegniasis on Fish by Using In Vitro]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v7i2.11196 ◽

2019 ◽

Vol 7 (2) ◽

pp. 133

Author(s):

Oktantia Frenny Anggani ◽

Rahayu Kusdarwati ◽

Hari Suprapto

Keyword(s):

Bacillus Licheniformis ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Streptomyces Olivaceoviridis ◽

Tukey Honestly Significant Difference

Abstract Saprolegniasis is a fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging healthy tissue and makes the immune system in fish deacreased. The uniquennes of Saprolegnia sp has the main components of the cell wall in the form of chitin that was instrumental in shaping the structure of the tip growth of fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic microorganisms based on ability to produce chitinase for example using bacteria. This study aims to potential of Bacillus licheniformis and Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp, cause saprolegniasis on fish by using in vitro. The research method is experimental with completely randomized design (CRD), which consists of four treatments with five replications. The experimental used is A (Saprolegnia sp and Bacillus licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol 2 %). The main parameters measured were observed inhibition zone on each treatment. Supporting parameters were observed is an observation of abnormal hpyphae structure after being induced by bacteria Bacillus licheniformis dan Streptomyces olivaceoviridis. Data were analyzed using analysis of variants (ANOVA) and to know the difference between treatments were determined by Tukey honestly significant difference (Tukey HSD) Test. The results showed that the potential of chitinolytic Bacillus licheniformis can provide a good barrier of 4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the growth Saprolegnia sp. Suggestions in this research is the need to further research on the value of chitinase Bacillus licheniformis and Streptomyces olivaceoviridis. Further research by using in vivo.

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Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v3i3.22742 ◽

2018 ◽

Vol 3 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Suryadi Budi Utomo ◽

Mita Fujiyanti ◽

Warih Puji Lestari ◽

Sri Mulyani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Hexadecyltrimethylammonium Bromide ◽

E Coli ◽

Escherichia Coli Bacteria

This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against Staphylococcus aureus and Escherichia coli bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the S. aureus bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against E. coli.

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Tinjauan Kinetika Kimia Daya Hambat Minyak Daun Cengkeh (Syzygium aromaticum) dan Hasil Mikroenkapsulasinya Terhadap Eschericia coli

Indo J Chem Res ◽

10.30598//ijcr.2020.7-lai ◽

2020 ◽

Vol 7 (2) ◽

pp. 151-158

Author(s):

Laily - Nurliana ◽

Desi Kurniawati ◽

Rustam Musta ◽

Laode Abdul Kadir ◽

Fitria Dewi ◽

...

Keyword(s):

Rate Constant ◽

Reaction Order ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

Disk Diffusion Method ◽

E Coli ◽

Relationship Of

The secondary metabolite compounds contained in clove leaves oil have the potential to be antibacterial Escherichia coli. The kinetic analysis is based on the inhibition zone formed as a result of the inhibition of clove leaves oil and the results of microencapsulation on E. coli bacteria. The determination of the inhibition zone uses the disk diffusion method by using amoxicillin as a positive control and tween oil as a negative control. The kinetic review is intended to find out the antibacterial inhibitory reaction process by determining the reaction order (n), rate constant (k) as well as the At-Ao-t relationship of clove leaves oil and the results of its microencapsulation. Based on the research results obtained by the reaction order (n) of clove oil as an antibacterial E. coli is n = 0.1913; with the rate constant k = 5.7679. The reaction order (n) results of microencapsulation as antibacterial E. coli are 0.5039; with the rate constant k = 4.7323.

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Synthesis, Molecular Docking Analysis, and Evaluation of Antibacterial and Antioxidant Properties of Stilbenes and Pinacol of Quinolines

Advances in Pharmacological and Pharmaceutical Sciences ◽

10.1155/2021/6635270 ◽

2021 ◽

Vol 2021 ◽

pp. 1-17

Author(s):

Zeleke Digafie ◽

Yadessa Melaku ◽

Zerihun Belay ◽

Rajalakshmanan Eswaramoorthy

Keyword(s):

Molecular Docking ◽

Biological Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Inhibition Zone ◽

Positive Control ◽

Health Concern ◽

Diffusion Method ◽

E Coli

Emergence of antimicrobial resistance to standard commercial drugs has become a critical public health concern worldwide. Hence, novel antimicrobials with improved biological activities are urgently needed. In this regard, a series of quinoline-stilbene derivatives were synthesized from substituted quinoline and benzyltriphenylphosphonium chloride using Wittig reaction. Furthermore, a novel pinacol of quinoline was synthesized by pinacolinazation of 2-methoxyquinoline-3-carbaldehyde which was achieved by aluminum powder-potassium hydroxide reagent combination at ambient temperature in methanol. The structures of the synthesized compounds were established based on their spectral data. The antibacterial activities of the synthesized compounds were evaluated in vitro by the paper disc diffusion method against two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two Gram-negative bacteria (Escherichia coli and Salmonella typhimurium). The best activity was displayed by compound 19 against E. coli with an inhibition zone of 16.0 ± 0.82 mm and 14.67 ± 0.94 mm at 500 and 250 μg/mL, respectively. This is close to ciprofloxacin which is used as a positive control. The results of in silico molecular docking evaluation of the compounds against E. coli DNA gyraseB were in good agreement with the in vitro antibacterial analysis. Compounds 19 (−6.9 kcal/mol) and 24 (−7.1 kcal/mol) showed the maximum binding affinity close to ciprofloxacin (−7.3 kcal/mol) used as positive control. Therefore, the antibacterial activity displayed by these compounds is encouraging for further investigation to improve the activities of quinoline-stilbenes by incorporating various bioisosteric groups in one or more positions of the phenyl nuclei for their potential pharmacological use. Findings of the DPPH radical scavenging assay indicated that some of the quinolone stilbenes and pinacol possess moderate antioxidant properties compared to ascorbic acid used as a natural antioxidant.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

Jurnal Natur Indonesia ◽

10.31258/jnat.13.1.63-66 ◽

2012 ◽

Vol 13 (1) ◽

pp. 63

Author(s):

Taswin Yacob ◽

Rita Endriani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethanol Extract ◽

Positive Control ◽

Diffusion Method ◽

Randomized Design ◽

Senna Alata ◽

Treatment Of Infection ◽

Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

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STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS

Wiadomości Lekarskie ◽

10.36740/wlek202109115 ◽

2021 ◽

Vol 74 (9) ◽

pp. 2109-2111

Author(s):

Evheniia A. Shtaniuk ◽

Oleksandra O. Vovk ◽

Larisa V. Krasnikova ◽

Yuliia I. Polyvianna ◽

Tetiana I. Kovalenko

Keyword(s):

Antibacterial Activity ◽

Water Soluble ◽

Diffusion Method ◽

E Coli ◽

Mueller Hinton ◽

Microbiological Methods ◽

Pure Cultures ◽

Inflammatory Processes ◽

Definition Of

The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them. Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method (“well” method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis. Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine. Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.

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