Standardisasi Simplisia dan Ekstrak Daun Matoa (Pometia pinnata J.R Forst & G. Forst) Asal Kalimantan Selatan

2021 ◽  
Vol 8 (1) ◽  
pp. 101
Author(s):  
Sutomo Sutomo ◽  
Norijatil Hasanah ◽  
Arnida Arnida ◽  
Agung Sriyono
Keyword(s):  
Bitter Taste ◽  
Botanical Garden ◽  
Ash Content ◽  
Water Soluble ◽  
Cell Nuclei ◽  
Soluble Extract ◽  
Green Color ◽  
Medicinal Plant Extracts ◽  
Water Soluble Extract ◽  
Ethanol Extracts

Matoa (Pometia pinnata) merupakan salah satu  tumbuhan yang dapat tumbuh dengan baik di Kalimatan dan diketahui mengandung metabolit sekunder yang berpotensi sebagai obat. Penelitian ini bertujuan untuk melakukan standardisasi berdasarkan parameter spesifik dan nonspesifik dari simplisia dan ekstrak. Pengambilan sampel daun P. pinnata dilakukan pada tiga tempat tumbuh yaitu Desa Pemuda, Kebun Raya Banua, dan Tahura Sultan Adam. Metode standardisasi yang digunakan mengacu pada Farmakope Herbal Indonesia dan Parameter Standar Umum Ekstrak. Pengamatan organoleptik simplisia yaitu berwarna hijau muda, rasa pahit, berbau khas. Pengamatan mikroskopik menunjukkan adanya dinding sel, floem, xilem, stomata, dan inti sel. Kadar sari larut etanol (19,07-19,27)%; kadar sari larut air (20,93-21,17)%; susut pengeringan (6,17-6,23)%; kadar abu total (4,63-4,73)%; kadar Pb (0,014-0,022) ppm; kadar Cd (0,014-0,015)ppm; dan kadar Hg <0,00004ppm. Pemerian ekstrak yaitu berwarna hijau kehitaman, berbau khas, rasa pahit. Ekstrak etanol daun P. pinnata mengandung alkaloid, flavonoid, steroid, tanin, glikosida, saponin, antrakuinon yang ditegaskan pada profil KLT menunjukkan kesamaan kandungan senyawa kimia pada tiap tempat tumbuh. Rendemen yang didapat (11,19-14,68)%; kadar air (5,57-5,97)%; kadar abu total (1,19-1,24)%; dan kadar abu tidak larut asam (0,41-0,44)%. Hasil uji parameter spesifik dan nonspesifik simplisia dan ekstrak daun P. pinnata dari tiga tempat tumbuh telah memenuhi syarat yang ditetapkan oleh MMI dan BPOM RI. Kata Kunci :  Standardisasi; Matoa; Pometia pinnata, Simplisia; Ekstrak   Matoa (Pometia pinnata) is one of the plants that can grow well in Kalimantan and known to contain efficacious secondary metabolites. This study is aimed to determine the value of specific parameters and nonspecific parameters of simplicia and extract. The leaves of P. pinnata was carried out in three growing places, which are the Pemuda Village, Banua Botanical Garden, and Tahura Sultan Adam. The method of determining the standardization parameters refers to Indonesian Herbal Pharmacopoeia and the General Standard Parameters of Medicinal Plant Extracts. The result from organoleptic observations of simplicia showed that P. pinnata had a light green color, a bitter taste, and a distinctive smell. Microscopic observations showed phloem, xylem, stomata, cell nuclei, cell walls. The content of ethanol soluble extract was 19.07%-19.27%, water soluble extract content was 20.93%-21.17%, drying losses was 6.17%-6.23%, total ash content was 4.63%-4.73%, Pb levels was 0.014-0.022ppm, Cd levels was 0.014-0.015ppm, and Hg levels was <0.00004 ppm. The description of extracts was that P. pinnata had a blackish green color, a bitter taste, and a distinctive smell. Ethanol extracts of P. pinnata leaves contained alkaloids, flavonoids, steroids, tannins, glycosides, saponins, anthraquinones which were confirmed in the TLC profile  showing the similarity of chemical compounds in each growing place. The yield extract was 11.19%-14.68%, water content was 5.57%-5.97%, total ash content was 1.19%-1.24%, and acid insoluble ash content was 0.41%-0.44%. The results of specific and nonspecific parameters of simplicia and P. pinnata leaf extract from three growing sites have met the requirements set by MMI and BPOM RI.

scholarly journals FORMULASI SEDIAAN KRIM ANTI LUKA BAKAR DARI EKSTRAK AIR DAGING DAUN ALOE VERA

Jurnal Kimia ◽  
2020 ◽  
pp. 37
Author(s):  
P. O. Samirana ◽  
N. W. Satriani ◽  
P. R. Harfa ◽  
S. P. P. Dewi ◽  
C. I. S. Arisanti
Keyword(s):  
Aloe Vera ◽  
Water Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Meat Extract ◽  
Chemical Screening ◽  
Extract Content ◽  
Water Soluble Extract

Aloe vera (Aloe vera) is a plant that is empirically often used to heal burns. Aloe vera leaf meat water extract contains saponins and flavonoids, in addition it also contains tannins and polyphenols. This research was conducted to determine whether the extracted water of Aloe vera leaf meat had met the parameters of extract quality standards so that it could be used in formulations. The steps taken are the extraction of aloe vera leaf meat with the method of infundation maceration, standardization of Aloe vera meat water extraction including testing the determination of drying shrinkage, total ash content, determination of ash content which is insoluble in acid, determination of the essence of water soluble extract, determination of the extract soluble in ethanol and chemical screening, identification with FT-IR, preparation of cream preparations, evaluation of cream preparations. Aloe vera leaf meat extract was obtained by infudation technique. Tests for drying drying extract produced 26.33%, total ash content of 1.3%, water soluble extract content of 11.9% and ethanol soluble extract content of 12.01%, total flavonoid content of 2.9%.   Keywords: Aloe Vera, Formulation, Cream, Burns.

scholarly journals KARAKTERISASI PARAMETER SPESIFIK DAN PARAMETER NON SPESIFIK AKAR KUNING (Fibraurea tinctoria)

2020 ◽  
Vol 5 (2) ◽  
pp. 416-425
Author(s):  
Supomo Supomo ◽  
◽  
Hayatus Sa`adah ◽  
Eka Siswanto Syamsul ◽  
Kintoko Kintoko ◽  
...  
Keyword(s):  
Water Content ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Anticancer Properties ◽  
East Kalimantan ◽  
Extract Content ◽  
Water Soluble Extract ◽  
Cell Fragments

One of the secondary metabolites from yellow root (Fibraurea tinctoria) that has the potential to be developed as medicine is berberine. Alkaloid class compounds are reported to have antimicrobial, anti-diarrheal, intestinal parasite infection inhibitors, antihypertensives, anti-tumor, anti-inflammatory, hepatoprotective, antimalarial and anticancer properties. In the development of natural ingredients as medicine, it is necessary to standardize simplicia and extracts The research objective was to determine the characterization of specific and non-specific parameters of yellow root plants. This research is an experimental research. The object of research used yellow roots obtained from KDKT Samboja, Kutai Kartanegara district, East Kalimantan Province. Samples were made of simplicia which were extracted using the maceration method with 70% ethanol solvent. The results of the extraction were carried out by characterization including: macroscopic, microscopic, water content, water-soluble extract, ethanol-soluble extract, ash content, and acid-insoluble ash, then phytochemical screening was carried out. Data were analyzed using descriptive methods. The results of macroscopic characterization of yellow root simplicia powder were Liana with a length of 10-20 m, with round stems, bark grayish brown, coarse, 2-7 cm in diameter and bright yellow powder color. The simplicia powder microscopy found stone cell fragments and tracheal fragments. Characterization of simplicia powder, water content of 67.3%, ethanol soluble extract content of 6.69%, water soluble extract content of 8.05%, ash content of 7.37%, acid insoluble ash content of 0.37%. The results of the identification of the chemical compound group were positive for containing chemical compounds in the form of alkaloids, flavonoids and saponins.

scholarly journals Extraction And Phytochemical Screening of Ethanol Extract And Simplicia of Moringa Leaf (Moringa Oleifera Lam.) From Sidikalang, North Sumatera

2021 ◽  
Vol 2 (6) ◽  
pp. 2072-2076
Author(s):  
Ricky Andi Syahputra ◽  
Ani Sutiani ◽  
Pasar Maulim Silitonga ◽  
Zulmai Rani ◽  
Amelia Kudadiri
Keyword(s):  
Ethanol Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Phytochemical Screening ◽  
Soluble Extract ◽  
Tropical Plant ◽  
Extract Content ◽  
Leaf Powder ◽  
Water Soluble Extract

Moringa leaf is a plant that has many uses that comes from the family Moringaceae and it is a tropical plant that is familiar to Indonesian people. Pharmacologically, this plant extract is reported to have antimicrobial and fungicide and it is rich in antioxidants. This metabolite compound contained in Moringa leaves has the potential as an antioxidant, antibacterial, functional and others. This study aims to determine the simplicia characterization of Moringa leaves and to determine the secondary metabolites contained in Moringa leaves, both Moringa leaf powder and Moringa leaf extract. The method of this research is experimental including the simplicia making and ethanol extract of Moringa leaves by maceration method, simplicia characterization and phytochemical screening. The results of the simplicia characterization of Moringa leaves for ethanol soluble extract content was 10,9% and water-soluble extract content was 15,8%, ash content was 9,6% and acid insoluble ash content was 0,6% and water content was 8%. The results of this study also showed that the simplicia powder and ethanol extract of Moringa leaves contained flavonoid, tannin, alkaloid, steroid and saponin.

scholarly journals Standardization of Phyllanthus niruri and Sonchus arvensis as Components of Scientific Jamu

2020 ◽  
Vol 25 (1) ◽  
pp. 7
Author(s):  
Nikmatul Ikhrom Eka Jayani ◽  
Alfian Hendra Krisnawan ◽  
Nina Dewi Oktaviyanti ◽  
Kartini Kartini
Keyword(s):  
Ash Content ◽  
Water Soluble ◽  
Service Research ◽  
Soluble Extract ◽  
Phyllanthus Niruri ◽  
Standard Requirement ◽  
Extract Content ◽  
Crude Drugs ◽  
Water Soluble Extract ◽  
Sonchus Arvensis

Saintifikasi Jamu, or the scientific investigation of Jamu, is an evidence-based process to ensure the safety and efficacy of herbal medicine through health service research. Jamu has been empirically explored as a composition of various crude drugs, hence called as Scientific Jamu. Phyllanthus niruri and Sonchus arvensis are two of the 30 medicinal plants processed into Scientific Jamu. Components of the Scientific Jamu are standardized to ensure that these materials meet the predefined quality. This study was aimed to determine the specific parameters (macroscopic, microscopic, total flavonoid content, water-soluble extract content, and ethanol-soluble extract content) and non-specific parameters (loss on drying, total ash content, and acid insoluble ash content) of Phyllanthus niruri and Sonchus arvensis collected from Batu, Tawangmangu, and Bogor. The methods of the determination referred to the Indonesian Herbal Pharmacopoeia 1st Edition (2008). The results showed that Phyllanthus niruri and Sonchus arvensis from the three places did not meet the standard requirement.

scholarly journals The Potency of Hibiscus rosa-sinensis Linn. Leaves Ethanol Extract as Hair Growth

2020 ◽  
Vol 8 (A) ◽  
pp. 89-92
Author(s):  
Imam Budi Putra ◽  
Nelva K Jusuf ◽  
Imam Bagus Sumantri
Keyword(s):  
Hair Growth ◽  
Ethanol Extract ◽  
Average Concentration ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Hibiscus Rosa Sinensis ◽  
Extract Content ◽  
Ethanol Extracts

INTRODUCTION: Hair is one of the organs and crowns for all people. Hair loss will really bother for most people. One of the plants that can be used as hair growth is Hibiscus rosa-sinensis Linn. OBJECTIVE: The objective of the study was to find the potency of H. rosa-sinensis leaves ethanol extracts as hair growth. METHODS: In this research, simplicia and extracts were made from hibiscus leaves using ethanol as a solvent. Simplicia and extracts were tested for phytochemical screening and characterization of each. The extract obtained was tested in vivo with animals test by observing mouse’s hair growth for 25 days of observation. RESULTS: Simplicia and ethanol extract of Hibiscus leaves contain flavonoids, alkaloids, glycosides, tannins, and steroids/terpenoids. The characteristics of Hibiscus leaves simplicia and extract had water content (9.71% vs. 10.65%), water-soluble extract content (19.91% vs. 27.58%), ethanol-soluble extract content (37.86% vs. 49.51%), total ash content (7.96% vs. 9.46%), and acid-insoluble ash content (0.78% vs. 1.28%). In vivo testing with animals, ethanol extract of Hibiscus leaves had an effect as a hair growth until the 25th day with an average concentration of 2.5% (14 mm), 5% (16 mm), and 10% (19 mm). DISCUSSION: The extract of Hibiscus leaves that showed the presence of carbohydrate, alkaloid, flavonoid, steroids, protein, tannin, and amino acids. The presence of active constituents such as flavonoids and tannins may be responsible for hair growth activity. CONCLUSION: The ethanol extracts of H. rosa-sinensis leaves can increase hair growth, at optimum concentrations of 10% extract.

scholarly journals Physical Stability of Hair Tonic Contain Ethanol Extract Galangal (Alpinia galanga L.) Rhizome and Aloe Vera Leaf Filtrate (Aloe vera L.)

2020 ◽  
Vol 6 (2) ◽  
pp. 67
Author(s):  
Nur illiyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
Rahiswari Pramudita Lakasa ◽  
Suryani Suryani ◽  
Ari Sartinah ◽  
...  
Keyword(s):  
Aloe Vera ◽  
Ethanol Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Stability Testing ◽  
Soluble Extract ◽  
Alpinia Galanga ◽  
Cycling Test ◽  
Before And After ◽  
Water Soluble Extract

AbstractFormulation and stability testing of hair tonic contain galangal rhizome (Alpinia galanga L.) ethanol extract and Aloe vera L. filtrate has been done as alternative for dandruff removal and hair growth. This study aimed to produce stable hair tonic. Galangal rhizome extract obtained by maceration method using ethanol and aloe leaf filtrate obtained by filtering. Extracts and filtrates characterized include water and ethanol soluble content, water and ash content. Hair tonic was formulated by mechanical mixing method with variations of galangal rhizome ethanol extract and aloe vera leaf filtrate which were 4% and 12.5%; 5% and 25%; 6% and 37.5%. Hair tonic prepared were tested for stability by cycling test method including organoleptic, homogeneity, pH, and viscosity. The characteristics of the galangal rhizome ethanol extract were 12% water soluble extract, 12% ethanol soluble extract, 3% water content, and 2% ash content. The characteristics of Aloe vera leaf filtrate were 10% ethanol soluble extract, 1.6% water soluble extract, and 1% ash content. Stability testing shows that hair tonic preparations are organoleptically stable and homogeneous. The pH values before and after cycling test for formulas A, B, and C were 5.27 to 5.34, respectively; 5.00 to 5.15; and 4.87 to 5.05. Viscosity before and after the cycling test for formulas A, B, and C were 1.178 to 1.676 cPs respectively; 1,306 to 1,883 cPs; and 2,148 to 2,296 cPs. It is concluded that hair tonic prepared are stable based on the requirements of Indonesian National Standard (SNI).

scholarly journals Standardization of Simplicia and Ethanol Extract of Purun Danau (Lepironia articulata (Retz.) Domin) Rhizome

2021 ◽  
Vol 4 (4) ◽  
pp. 273-282
Author(s):  
Arnida Arnida ◽  
Maulidia Maulidia ◽  
Amalia Khairunnisa ◽  
Sutomo Sutomo ◽  
Faisal Faisal
Keyword(s):  
Drying Shrinkage ◽  
Ethanol Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Extract Content ◽  
Standard Parameter ◽  
Water Soluble Extract ◽  
Specific Odor ◽  
General Standard

Purun Danau (Lepironia articulata (Retz.) Domin) has been shown to have antimalarial and antioxidant activity. This study aimed to standardize simplicia and extract of L. articulata by determining the value of its specific and non-specific parameters. A sampling of L. articulata rhizome was carried out in Guntung Manggis, Haur Gading, and Halat. The standardization method used was based on the Indonesian Herbal Pharmacopoeia and the General Standard Parameter of Extract. The organoleptic observations showed that L. articulata simplicia was reddish-brown in color, chelated taste, and had a specific odor. Microscopic observation showed parts of this plant: epidermis, cortex, endodermis, parenchyma, bundle vessels, and scalariform vessels. Ethanol-soluble extract content was 10.00-12.66%, water-soluble extract content 8.03-10.87%, drying shrinkage 7.10-7.33%, total ash content 2.03-2.52%, acid-insoluble ash 0.33-0.42%, Pb content 5.698-9.989 ppm, Cd content 0.300-0.500 ppm, Hg content 0.070-0.090 ppm. Ethanol extract of L. articulata rhizome contained alkaloids, flavonoids, tannins, glycosides, and saponins. The yield obtained was 8.05-11.23%, total ash content was 1.58-1.67%, acid-insoluble ash was 0.23-0.33%, and water content was 7.10-8.50%. Standardization of simplicia and ethanol extract of L. articulata rhizome has met the criteria.

Standardization and bacteria inhibitory test of purified extract of mahogany (Swietenia mahagoni (L.) Jacq) seeds and leaves

2019 ◽  
Vol 10 (3) ◽  
pp. 2132-2138
Author(s):  
Virsa Handayani ◽  
Rezki Amriati Syarif ◽  
Ahmad Najib ◽  
Aktsar Roskiana Ahmad ◽  
Abdullah Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Bitter Taste ◽  
Drying Shrinkage ◽  
Ash Content ◽  
Water Soluble ◽  
Lead Contamination ◽  
Specific Parameter ◽  
Chemical Content ◽  
Swietenia Mahagoni

Mahogany (Swietenia mahagoni (L.) Jacq) is one of the plants that is often used by the community as traditional medicine. One of them is antifungal, antibacterial, antidiabetic, and eczema. This study aims to obtain standardized extracts from mahogany seeds and leaves. Standardization of purified extract of mahogany has been carried out according to the monographs of extract standardization guidelines, which include testing of specific and non-specific parameters. The results of the specific parameter testing showed that the purified extract of mahogany seeds is a thick extract, brown to reddish, smells distinctive and has a bitter taste. While the purified extract of mahogany leaves is a thick extract, greenish-brown in color, distinctive smell and has a bitter taste. The chemical content of purified extract of mahogany seeds and leaves showed the presence of flavonoids, alkaloids, terpenoids and saponins. Water-soluble essence levels in mahogany seeds and leaves was 14.84% and 10.28%. While the ethanol-soluble essence levels in mahogany seeds and leaves were 15.38% and 12.43%. Testing of non-specific parameters on mahogany seeds and leaves showed the results of drying shrinkage levels of 0.22% and 8.84%, moisture content of 2.60% and 4.04%, total ash content of 1.71% and 1.93%, levels acidic insoluble ash 0.38% and 0.32%, Total Plate Number (ALT) of mahogany seed bacteria 1x102 colonies/g, Number of mahogany mold seeds 4x10 colonies/g, heavy metal lead contamination and cadmium in mahogany seeds 0.0607µg/g and<0.003µg/g. The inhibitory diameter of each concentration of seeds against Escherichia coli, 3%, 5%, 7%, and 9%, is 12,67; 13,67; 17,67; and 19,67 mm, respectively. The inhibitory diameter of each concentration of leaves against Escherichia coli, 3%, 5%, 7%, and 9%, is 10,27; 10,90; 13,46; and 15,68 mm, respectively.

Prebiotic frozen dessert processed with water‐soluble extract of rice byproduct: Vegan and nonvegan consumers perception using preferred attribute elicitation methodology and acceptance

2021 ◽  
Vol 86 (2) ◽  
pp. 523-530
Author(s):  
Jiuliane Martins da Silva ◽  
Carlos Eduardo Barão ◽  
Erick Almeida Esmerino ◽  
Adriano Gomes Cruz ◽  
Tatiana Colombo Pimentel
Keyword(s):  
Water Soluble ◽  
Soluble Extract ◽  
Frozen Dessert ◽  
Water Soluble Extract

Activation of Selective Transcription Factors and Cytokines by Water-Soluble Extract from Lentinus lepideus

2003 ◽  
Vol 228 (6) ◽  
pp. 749-758 ◽  
Author(s):  
Mirim Jin ◽  
Hyung Jin Jung ◽  
Jeong June Choi ◽  
Hyang Jeon ◽  
Jin Hwan Oh ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Post Treatment ◽  
Water Soluble ◽  
Dependent Manner ◽  
Soluble Extract ◽  
Transient Transfection Assay ◽  
Gm Csf ◽  
Tnf Α ◽  
Water Soluble Extract

We isolated a water-soluble extract, PG101, from cultured mycelia of Lentinus lepideus. Treatment of human peripheral blood mononuclear cells (PBMCs) with PG101 increased levels of TNF-α, IL-1β, IL-10, and IL-12 by 100- to 1000-fold, whereas GM-CSF and IL-18 were activated by an order of magnitude. On the contrary, IFN-γ and IL-4 were not affected. The response to PG101 occurred in a dose- and time-dependent manner. From the human PBMCs treated with PG101, TNF-α was a first cytokine to be activated, detectable at 2 hr post-treatment followed by IL-1β at 6 hr post-treatment. IL-12 and IL-10 were the next to follow. GM-CSF and IL-18 both showed significant increases 24 hr after treatment. When PBMCs were sorted into various cell types, monocyte/macrophages, but not T and B cells, were the major target cell type responsive to PG101. Consistent with this result, the profile of cytokine expression upon PG101 treatment was comparable between PBMCs and a human promonocytic cell line (U937), whereas cell lines of T cell and myeloid origins did not respond to PG101. Data from a transient transfection assay involving specific reporter plasmids indicated that cellular transcription factor such as NF-κB, but not AP-1, was highly activated by PG101. Results from a gel retardation assay and the experiment involving a specific NF-κB inhibitor confirmed the involvement of NF-κB. Despite its significant biological effect on various cytokines, PG101 remained nontoxic in both rats and PBMCs even at a biological concentration approximately 20 times greater. PG101 demonstrates great potential as a therapeutic immune modulator.

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