scholarly journals To Study the Effect of Auxin Concentrations (IBA and IAA) on Growth Performance of Stem Cutting of Hardwickia binata (Roxb.)

2021 ◽  
Vol 10 (11) ◽  
pp. 243-249
Author(s):  
Pavani Ramavath Afaq Majid Wani ◽  
M. Shiva Kumar
Keyword(s):  
Growth Performance ◽  
Stem Cutting ◽  
Controlled Environment ◽  
Stem Cuttings ◽  
Environmental Condition ◽  
Clonal Forestry ◽  
Survival Percentage ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Mist Chamber

The present studies on “Effect of Plant Growth Regulators on Growth Performance of Stem Cutting of Hardwickia binata Roxb” was carried out in the mist chamber of Institute of Forest Biodiversity, Dulapally, Kompally, Hyderabad, during January- April, 2021. The cuttings of Hardwickia binata Roxb were prepared from selected superior phenotypes from the surrounding environments of Kompally, Hyderabad. Hardwickia binata Roxb. is difficult to root and need auxin treatment and controlled environment to successful survival and rooting. The cuttings were treated with 0, 200, 500,1000, 1500, 2000 mg/l concentrations of IBA and IAA and planted in poly bags under mist chamber. The experiment was conducted in a completely randomized design. Analysis of variance was worked out to assess the variation in Hardwickia binata Roxb. for eight characters. Treatment with IBA at 2000mg was found to be the best treatment as it achieved maximum in survival percentage (52.64%), shoot length (31.92cm), rooting percentage (81.69%), number of roots per cutting (20.44) and root biomass (6.32g) followed by treatment with IAA at 2000mg/l (T10) achieved maximum. The performance increased with an increase in concentrations of both IBA and IAA. Among the two auxins treatments, IBA was found to be most effective, showed significantly higher values compared to IAA. It is possible to successfully multiply the Hardwickia binata Roxb. cuttings by treating with 2000 mg/l of IBA under controlled phyto-environmental condition. The study evolved an easy and efficient protocol for vegetative propagation of HardwickiabinataRoxb.via stem cuttings to establish clonal forestry.

scholarly journals Assessing Rooting Media and Hormone on Rooting Potential of Stem Cuttings of Bougainvillea

2018 ◽  
Vol 35 (1) ◽  
pp. 197-201
Author(s):  
P. Marasini ◽  
A . Khanal
Keyword(s):  
Root Length ◽  
Garden Soil ◽  
Controlled Environment ◽  
Stem Cuttings ◽  
Factor B ◽  
Randomized Design ◽  
Bud Sprouting ◽  
Rooting Media ◽  
Completely Randomized Design ◽  
Four Levels

 An experiment was conducted at Lamjung Campus, Lamjung to assess the rooting performance and bud sprouting of Bougainvillea, an evergreen ornamental vine. The study was conducted in partially controlled environment with two factorial treatments considering factor A in four levels(coco peat + sand, sand + FYM, garden soil, garden soil + FYM and garden soil )and factor B (with and without NAA 3000ppm) were arranged in completely randomized design (CRD) with three replication during September- November, 2016. 18 cuttings having 15 cm in length with 4 buds were planted in each treatment. Root length (7.83cm) found in Sand & FYM was significantly different to other media but rooting percentage (77.77%) was found statistically at par with other media except garden soil. Effect of hormone was found non-significant on total sprouted plant, sprout diameter and number of sprouts/cuttings whereas other parameters were affected significantly. Interaction effect of rooting media and hormone significantly affects root length (11.6cm) and rooting percentage (83.33%) which was found on sand & FYM along with NAA 3000ppm followed by Garden soil & FYM in combination with NAA 3000ppm while others parameters were non-significant. Thus it is advisable to use a good rooting media along with application of NAA 3000 ppm for better rooting in cuttings.

scholarly journals Penggunaan Air Kelapa Untuk Setek Batang Jati (Tectona Grandis)

2016 ◽  
Vol 4 (1) ◽  
pp. 61
Author(s):  
Rega Renvillia ◽  
Afif Bintoro ◽  
Melya Riniarti
Keyword(s):  
Growth Regulator ◽  
Water Immersion ◽  
Tectona Grandis ◽  
Stem Cutting ◽  
Coconut Water ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Root Cutting ◽  
Water Cutting ◽  
Completely Randomized Design

Teak plant wasa treewhich timber has durability classI and strong class I.  It hasmany benefits, from roots to leaves. Variousbenefits of teak causing to do cultivation, one of propagation activities that often carried out was vegetative. Vegetative propagation of plants can be done bystem cutting, root cutting and shoot cutting.  Using of growth regulator with coconut water immersion is expected to spur the growth rootofteak stem cutting. This study aimed to determine the effect of using coconut water to increase the success of teak stem cuttinggrowth, andto determine the best dose of using coconut water forteak stem cutting growth. This treatment used Completely Randomized Design(CRD).  This research consisted of five treatments that usedcoconut water treatment 0%, 25%, 50%, 75%, and 100%. Each treatmentconsisted of five teak stem cuttings with repetition as much as 5 times.  Amount ofmaterial plants required 5 x 5 x 5 = 125 teak stem cuttings.  The resultshowed by using coconut wateras a growth regulator could increasethe growth of teak (Tectona grandis) stem cuttingin 50%--100% concentration and the best dose of coconut water asa growth regulator was 100%.  Keywords : coconut water, cutting, dose, growth regulator, teak

THE SOURCES OF AUXIN HORMONE TO GROWTH OF SHOOT PINEAPPLE STEM CUTTING (Ananascomosus.L. Merr.)

2016 ◽  
Vol 1 (1) ◽  
pp. 45-52
Author(s):  
Palupi Puspitorini
Keyword(s):  
Water Concentration ◽  
Urine Concentration ◽  
Experimental Results ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Natural Hormone ◽  
Cow Urine ◽  
Urine Concentrations ◽  
Completely Randomized Design ◽  
Better Than

The aim of this study was to select the best sources of auxin of which it can stimulate the growth of shoots Pineapple plant cuttings. This research is compiled in a completely randomized design (CRD) with 4 treatments and 6 replications. The Data were statistically Analyzed by the DMRT. Level of treatment given proves that no treatment 0%, cow urine concentration of 25%, young coconut water concentration of 25% and Rootone F 100 mg / cuttings. The results showed that cow urine concentrations of 25% and Rootone F 100 mg give the best results in stimulating the growth of shoots pineapple stem cuttings. Experimental results concluded that the effect of this natural hormone were better than the shoots without given hormone.           

scholarly journals Cellulase hydrolyzed Indigofera zolingeriana leaf utilization as a feed ingredient for gourami fingerling

2021 ◽  
Vol 20 (2) ◽  
pp. 139-147
Author(s):  
Jefry Jefry ◽  
Mia Setiawati ◽  
Dedi Jusadi ◽  
Ichsan Achmad Fauzi
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Specific Growth ◽  
Second Step ◽  
Feed Ingredient ◽  
Protein Retention ◽  
Cellulase Enzyme ◽  
Randomized Design ◽  
Best Value ◽  
Completely Randomized Design

This study was aimed to evaluate the utilization of hydrolyzed Indigofera zolingeriana by celullase enzyme as the feed ingredient of gourami fish. This study used a completely randomized design which contained three steps, whereas each step contained four treatments and four replications. The first step performed by evaluating the Indigofera leaf meal (ILM) added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of ILM on gourami seeds. The third step was feed evaluation added with ILM as much as 0% (control), 15%, 30%, and 45% against the growth performance on gourami seeds. The gourami seeds used in the second and third steps with a weight of 13.65 ± 0.39 g/seed and 5.95 ± 0.15 g/seed, respectively. The addition of 0.8 g/kg and 1.2 g/kg cellulase enzyme could significantly decrease the crude fiber of ILM with 43.33%, besides having the best value of total, ingredient, protein, lipid, and energy digestibility. The growth performance of gourami seeds given 15% ILM added feed had the best value and insignificantly different from the control feed without ILM addition based on the specific growth rate (SGR), protein retention (PR), and feed efficiency (FE).          Keywords: Cellulase, feed, hydrolyze, Indigofera zolingeriana, Osphronemus gouramy.   ABSTRAK   Penelitian ini dilakukan untuk mengevaluasi pemanfaatan daun Indigofera zolingeriana yang dihidrolisis enzim selulase sebagai bahan baku pada pakan benih ikan gurami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas tiga tahap, dimana masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Pada tahap pertama dilakukan evaluasi tepung daun Indigofera (TDI) yang ditambahkan enzim selulase sebesar 0 g/kg (kontrol), 0.4 g/kg, 0.8 g/kg, dan 1.2 g/kg. Pada tahap kedua dilakukan uji kecernaan bahan TDI pada benih ikan gurami. Pada tahap ketiga dilakukan evaluasi pakan yang ditambahkan TDI sebesar 0% (kontrol), 15%, 30% dan 45%  terhadap kinerja pertumbuhan pada benih ikan gurami. Benih ikan gurami yang digunakan pada penilitian tahap kedua dengan bobot 13.65 ± 0.39 g/ekor dan 5.95 ± 0.15 g/ekor. Penambahan enzim selulase pada dosis 0.8 g/kg dan 1.2 g/kg secara signifikan mampu menurunkan serat kasar TDI sebesar 43.33 % dan memberikan nilai terbaik terhadap nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Kinerja pertumbuhan benih ikan gurami yang diberikan pakan yang ditambahkan TDI sebesar 15% memilki nilai terbaik dan tidak berbeda nyata terhadap pakan kontrol tanpa TDI dari aspek laju pertumbuhan spesifik (LPS), retensi protein (RP) dan efisiensi pakan (EP).   Kata kunci: Hidrolisis, Indigofera zolingeriana, Osphronemus gouramy, pakan, selulase.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals The evaluation of turmeric (Curcuma longa) supplementation within feed as an antioxidant towards growth performance of catfish Clarias gariepinus Burchell 1822 in zero water exchange condition

2020 ◽  
Vol 20 (2) ◽  
pp. 105
Author(s):  
Thoy Batun Citra Rahmadani ◽  
Dedi Jusadi ◽  
Mia Setiawati ◽  
Yuni Puji Hastuti
Keyword(s):  
Growth Performance ◽  
Liver Damage ◽  
Water Exchange ◽  
Curcuma Longa ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Randomized Design ◽  
Growth Performances ◽  
Exchange Condition ◽  
Completely Randomized Design

The objective of this research was to evaluate the supplementation of turmeric in the diet on antioxidant status and growth performance of African catfish (Clarias gariepinus) in zero water exchange condition. This study used a completely randomized design with 4 treatments and 4 replications. Each treatment consisted of feed supplementation turmeric at dosage of 0; 2.5; 5 or 7.5 g kg-1 diet. One hundred catfish juvenile (5.95±0.05 g) were stocked in intermediate bulk container (IBC) tank (1×1×1 m3) and rearing in zero water exchange condition for 60 days. Catfish were fed at satiation twice a day, in the morning and evening. The results showed that an increase in antioxidant content in catfish fed with the addition of turmeric, which simultaneously also reduced the percentage of liver damage. The parameters of liver damage can be seen from several parameters i.e. pale liver, droplet fat and fat content in the addition of turmeric treatment is lower than without the addition of turmeric. However, catfish fed with the addition of turmeric did not show significant results in terms of growth performances.

scholarly journals Growth performance and blood profile of rabbit bucks in two housing types on aqueous extract of oyster mushroom (Pleurotus ostreatus Jacq ex fr.)

2020 ◽  
Vol 46 (1) ◽  
pp. 51-60
Author(s):  
O. M. Sogunle ◽  
T. S. Aremu ◽  
O. O. Olatubosun ◽  
C. P. Njoku ◽  
O. M. Sogunle ◽  
...  
Keyword(s):  
Growth Performance ◽  
Pleurotus Ostreatus ◽  
Octadecadienoic Acid ◽  
Conversion Ratio ◽  
Oyster Mushroom ◽  
Feed Conversion ◽  
Blood Profile ◽  
Mushroom Extract ◽  
Randomized Design ◽  
Completely Randomized Design

The growth performance and blood profile of forty-eight (48), six (6) weeks old rabbit bucks of cross (Chinchilla and New Zealand White) were assessed for 10 weeks. The bucks were allotted on weight equalization basis in a 2 x 4 factorial experimental layout composed of 24 rabbits into steel hutches and wooden hutches each and on four (4) varying levels (0, 5, 10 and 15 mL/litre of water, respectively) of oyster mushroom (Pleurotus ostreatus Jacq ex fr.) extract. Six (6) bucks were assigned to each treatment group and replicated three times with two (2) bucks per replicate in a Completely Randomized Design. The phytochemicals of the oyster mushroom extract showed that 9,12-Octadecadienoic acid (Z, Z)- methyl ester was the most abundant. Results showed that the feed conversion ratio was significantly (P<0.05) best (6.08) in bucks reared in wooden cage and administered 15 mL oyster mushroom extract. Significantly (P<0.05) highest triglycerides (81.00 mg/dL) was obtained in bucks on steel hutch and on 5 mL oyster mushroom extract and lowest (55.00 mg/dL) in bucks on steel hutch and on 10 mL oyster mushroom extract. The lowest (P<0.05) lymphocytes (59.00%) were recorded in bucks on wooden cage and administered 10 mL oyster mushroom extract. It was concluded that oyster mushroom extract up to 15 mL/litre of water administered in either steel hutch or wooden hutch did not impair the growth performance and blood profile of rabbit bucks.

scholarly journals Carcass and organ characteristics of finishing broilers fed diets containing probiotics (Saccharomyces cerevisiae)

Agro-Science ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 25-30
Author(s):  
J.I. Ugwuoke ◽  
O.R. Okwesili ◽  
C.E. Dim ◽  
M.N. Okonkwo ◽  
H.M. Ndofor-Foleng
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Performance ◽  
Liver Weight ◽  
Heart Weight ◽  
Performance Indices ◽  
Randomized Design ◽  
Significant Difference ◽  
Ad Libitum ◽  
Completely Randomized Design ◽  
Effect Of Feeding

A five-week study was conducted to determine the effect of feeding varying levels of S. cerevisiae on carcass and organ characteristics of finishing broilers. One hundred and twenty 4-weeks old broilers of cobb strain were randomly assigned to four treatments (T1 = 0.6 g Saccharomyces cerevisiae (SC) kg–1 diet; T2 = 0.8 g SC kg–1 diet; T3 = 1.0 g SC kg–1 diet and T4 = 0.0 g SC kg–1 diet) with 30 birds per treatment and replicated twice with 15 birds per replicate in a completely randomized design. Feed and water were provided ad libitum to the birds in a deep litter system. In the end, data on growth, carcass and organ indices generated from the study were analyzed using one-way analysis of variance (ANOVA). Results showed no significant difference (p > 0.05) among the treatments in the birds’ growth performance indices. However, significant differences (p < 0.05) were observed in the birds’ values for liver weight, heart weight, shank length and thigh length with birds in T3 recording highest values of 61.30 g, 16.93 g and 12.00 cm for liver weight, heart weight and thigh length, respectively. It was thus concluded that finishing broilers fed 1.0 g of S. cerevisiae had superior carcass and organ characteristics than birds on the control and lower levels of inclusion.

scholarly journals Indolebutyric acid on rooting of peach hardwood cuttings

2018 ◽  
Vol 39 (5) ◽  
pp. 2273
Author(s):  
João Alison Alves Oliveira ◽  
Cláudio Horst Bruckner ◽  
Danielle Fabíola Pereira da Silva ◽  
Carlos Eduardo Magalhães dos Santos ◽  
Flávio Travassos Régis de Albuquerque Filho ◽  
...  
Keyword(s):  
Adventitious Rooting ◽  
Indolebutyric Acid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Mist Chamber ◽  
Root Quality ◽  
Plastic Trays

In the search for more efficient techniques for the propagation of peach cuttings, this study aimed to evaluate the rooting of hardwood cuttings of peach rootstock genotypes under different indolebutyric acid (IBA) concentrations. In the winter of 2016, the basal end of cuttings of genotypes 1701-1, 1701-2, 102-1, 102-2, 202-1 and 'Okinawa' were dipped into solutions with five concentrations of IBA (0, 1000, 2000, 3000 and 4000 mg mg L-1) for 5 seconds. The cuttings were then placed in sand in plastic trays and kept in a mist chamber. The experiment was arranged in a 6x5 factorial, completely randomized design, with 5 replications, and each plot consisted of eight cuttings. After 59 days, the variables related to rooting and root quality were evaluated. Genotypes 102-1 and 202-1 showed high adventitious rooting potential in hardwood cuttings, with 76.8 and 66.5% of rooting, respectively. The concentration of 2000 mg.L-1 of IBA acid can be recommended for the treatment of hardwood cuttings of the tested rootstocks for propagation in the winter.

scholarly journals Potency of Different Seaweeds as Diets for Developing Abalone (H. squamata) Culture in Nusa Penida Island, Bali

2018 ◽  
Vol 47 ◽  
pp. 02004
Author(s):  
Tri Heru Prihadi ◽  
Rasidi ◽  
Idil Ardi ◽  
Ani Widiyati ◽  
Dwi Budi Wiyanto
Keyword(s):  
Data Analysis ◽  
Experimental Design ◽  
Growth Performance ◽  
Shell Length ◽  
Economic Value ◽  
High Potency ◽  
Randomized Design ◽  
Variant Analysis ◽  
Completely Randomized Design ◽  
Seaweed Culture

Nusa Penida island is one of center for seaweed culture in Bali province. Seaweed that cultured is Euchema spinosum and Euchema cottoni spread around coastal. There also found wild seaweed, such as Ulva sp and Gracillaria sp. Abalone (H. squamata) is one of kind Mollusca which high economic value, but abalone cultured is not yet develop in Nusa Penida island, although this island have high potency for developing abalone culture. Abalone culture needs seaweeds as a diet. The abundance of seaweeds as abalon diet is important for developing abalon culture in Nusa Penida Island in the future. The aim of this research was to asses the potency of seaweeds as diet for developing abalone culture in Nusa Penida island. Research was done in Batununggul village, Nusa Penida Island. Experimental design was used Completely Randomized Design (CRD) with 3 treatments and 3 replications. The treatments were used different seaweeds as diet for abalone culture: (DG=Diet Gracillaria sp, DS=Diet Spinosum sp. and DU=Diet Ulva sp.). Abalon were cultured for 4 months by feeding the diet at satiation. Data analysis was used analysis varian (Anova) with SPSS. 16. The result showed abalon fed with Gracillaria sp (DG) at 4.73 g was the highest growth by 4.73 g, followed DU by 3.93 g and DS by 3.43 g. Meanwhile the abalon shell length fed with Gracillaria sp (DG) was the highest growth by 6.55 mm, followed DU by 5.97 mm and DS by 5.60 mm. Based on variant analysis showed growth performance (length shell and weight) abalon, all treatments were not significantly different (P>0.05). The conclusion this research, the three species of seaweed can be used as diet for abalone culture. These seaweeds have same potency as diet for developing abalone culture in Nusa Penida Island

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