scholarly journals Cellulase hydrolyzed Indigofera zolingeriana leaf utilization as a feed ingredient for gourami fingerling

2021 ◽  
Vol 20 (2) ◽  
pp. 139-147
Author(s):  
Jefry Jefry ◽  
Mia Setiawati ◽  
Dedi Jusadi ◽  
Ichsan Achmad Fauzi

This study was aimed to evaluate the utilization of hydrolyzed Indigofera zolingeriana by celullase enzyme as the feed ingredient of gourami fish. This study used a completely randomized design which contained three steps, whereas each step contained four treatments and four replications. The first step performed by evaluating the Indigofera leaf meal (ILM) added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of ILM on gourami seeds. The third step was feed evaluation added with ILM as much as 0% (control), 15%, 30%, and 45% against the growth performance on gourami seeds. The gourami seeds used in the second and third steps with a weight of 13.65 ± 0.39 g/seed and 5.95 ± 0.15 g/seed, respectively. The addition of 0.8 g/kg and 1.2 g/kg cellulase enzyme could significantly decrease the crude fiber of ILM with 43.33%, besides having the best value of total, ingredient, protein, lipid, and energy digestibility. The growth performance of gourami seeds given 15% ILM added feed had the best value and insignificantly different from the control feed without ILM addition based on the specific growth rate (SGR), protein retention (PR), and feed efficiency (FE).          Keywords: Cellulase, feed, hydrolyze, Indigofera zolingeriana, Osphronemus gouramy.   ABSTRAK   Penelitian ini dilakukan untuk mengevaluasi pemanfaatan daun Indigofera zolingeriana yang dihidrolisis enzim selulase sebagai bahan baku pada pakan benih ikan gurami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas tiga tahap, dimana masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Pada tahap pertama dilakukan evaluasi tepung daun Indigofera (TDI) yang ditambahkan enzim selulase sebesar 0 g/kg (kontrol), 0.4 g/kg, 0.8 g/kg, dan 1.2 g/kg. Pada tahap kedua dilakukan uji kecernaan bahan TDI pada benih ikan gurami. Pada tahap ketiga dilakukan evaluasi pakan yang ditambahkan TDI sebesar 0% (kontrol), 15%, 30% dan 45%  terhadap kinerja pertumbuhan pada benih ikan gurami. Benih ikan gurami yang digunakan pada penilitian tahap kedua dengan bobot 13.65 ± 0.39 g/ekor dan 5.95 ± 0.15 g/ekor. Penambahan enzim selulase pada dosis 0.8 g/kg dan 1.2 g/kg secara signifikan mampu menurunkan serat kasar TDI sebesar 43.33 % dan memberikan nilai terbaik terhadap nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Kinerja pertumbuhan benih ikan gurami yang diberikan pakan yang ditambahkan TDI sebesar 15% memilki nilai terbaik dan tidak berbeda nyata terhadap pakan kontrol tanpa TDI dari aspek laju pertumbuhan spesifik (LPS), retensi protein (RP) dan efisiensi pakan (EP).   Kata kunci: Hidrolisis, Indigofera zolingeriana, Osphronemus gouramy, pakan, selulase.

DEPIK ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 180-185
Author(s):  
Usman Bulanin ◽  
Yuneidi Basri ◽  
Sri Puja Lestari

The purpose of this study was to determine the addition of probiotics in commercial feed to the growth and survival of Asang fish (Osteochilus vittatus CV) fry. This study used an experimental method with a completely randomized design (CRD) with 4 treatments and 3 replications. The test fish used were 120 fish with a size ranging from 5-8 cm. The test fish were kept in a waring cage made of a type of paralon frame with a size of 40 x 40 x 45 cm. The treatment in this study was A (without probiotics), treatment B (addition of probiotic 10 ml/kg of feed), treatment C (addition of probiotic 20 ml/kg of feed), and treatment D (addition of probiotics 30 ml/kg of feed). From the results of this study, the highest specific growth rate was found in treatment D (6.66 ± 1.15 g), while the mean survival was 100% for all treatments. The highest FCR of fish fry was found in treatment A namely 1.43 ± 0.22, and the lowest in treatment D namely 1.12 ± 0.21, and for feed efficiency in treatment D, namely 95.24 ± 1.04 %.Keywords:Asang fishEfficiencyGrowthSurvival


2018 ◽  
Vol 39 (5) ◽  
pp. 2169
Author(s):  
Danielli Dos Santos Firmo ◽  
Marcos Antonio Delmondes Bomfim ◽  
Felipe Barbosa Ribeiro ◽  
Jefferson Costa de Siqueira ◽  
Eduardo Arruda Teixeira Lanna ◽  
...  

The objective of this study was to determine the optimal digestible threonine to lysine ratios in diets of tambaqui (Colossoma macropomum) juveniles. Five-hundred fish with a mean ± SE initial weight of 2.16 ± 0.03 g were used in a completely randomized design, consisting of four treatments, five replicates per treatment, and 25 fish per experimental unit. The treatments consisted of four isoenergetic, isophosphoric, isocalcic, and isolisinic (1.45%) diets, consisting of a basal diet supplemented with four L-threonine levels (1.013, 1.085, 1.158, and 1.230%), resulting in different threonine to lysine ratios (70, 75, 80, and 85%). Fish were maintained in twenty 500-L aquaria with independent water supply, drainage, and aeration systems, and were fed to apparent satiation six times a day for 45 days. Performance, feed efficiency, daily protein and fat deposition, body moisture content, and nitrogen retention efficiency of fish were evaluated. The digestible threonine intake increased linearly, and the efficiency of threonine for weight gain decreased quadratically, with increasing digestible threonine to lysine ratios. Weight gain, specific growth rate, feed:gain ratio, protein efficiency for weight gain, and nitrogen retention efficiency of fish increased in a quadratic manner with increasing digestible threonine to lysine ratios up to the levels of 75.96, 76.06, 76.36, 76.47, and 74.02%, respectively. It was concluded that the digestible threonine to lysine ratio for use in diets of juvenile tambaqui to achieve optimal performance and nitrogen retention efficiency is 76 and 74%, respectively, which corresponds to a digestible threonine level of 1.102 and 1.073%.


2020 ◽  
Vol 7 (2) ◽  
pp. 39
Author(s):  
Rani Andrianti Ekaputri ◽  
Muhammad Arief ◽  
Boedi Setya Rahardja

Litopenaeus vannamei is a leading commodity whose economic value. Targets of vannamei’s production increasing every year. One of important factor in the success of shrimp farming is feed. Research about feed fish directed to the creation of feed fish whose economical and safe for the environment, therefore is required an efforts to provide alternative feed through feed supplement. One of the material that can be supplemented in feed is chitosan. Chitosan can increase the specific growth rate and protein retention of vannamei. This study aims to determine the effect of chitosan supplementation in commercial feed for specific growth rate and protein retention of Litopenaeus vannamei. The research method used was completely randomized design (RAL) and performed with five treatments chitosan supplementation (0%, 1%, 2%, 3% dan 4%) experimental animal used was ten shrimps as five replication in each treatment. The main parameters were observed are specific growth rate and protein retention of vaname. Supporting parameters were observed is water quality. The results showed that chitosan supplementation in commercial feed for 30 days of maintenance give an effect on the specific growth rate and protein retention of vaname. The highest specific growth rate was obtained in treatment with 4% chitosan supplementation that not significantly different with treatment 3% chitosan supplementation. Protein retention was incresed in treatment with 4% chitosan supplementation that not significantly different with treatment 3% chitosan supplementation.


2019 ◽  
Vol 32 (3) ◽  
pp. 795-804
Author(s):  
THALLES JOSÉ REGO SOUSA ◽  
MARCOS ANTONIO DELMONDES BOMFIM ◽  
FELIPE BARBOSA RIBEIRO ◽  
SYLVIA SANAE TAKISHITA ◽  
DAYANA DA CANCEIÇÃO DA COSTA

ABSTRACT The objective of this study was to determine the digestible phosphorus requirement in rations for tambaqui fingerlings (Colossoma macropomum). 900 fingerlings (0.51 ± 0.06 g) were used in a completely randomized design with six treatments, five replicates, and thirty fish per experimental unit. The treatments consisted of six diets with different digestible phosphorus levels (0.12, 0.33, 0.54, 0.75, 0.95, and 1.16%). The fish were fed six times a day, for 63 days. Performance, feed efficiency, and the daily protein, fat, ash, and phosphorus deposition rates of fish were evaluated. The treatments did not influence the feed intake. The elevation of phosphorus levels increased the digestible phosphorus intake linearly, and improved of quadratic form the weight gain, specific growth rate, feed:gain ratio, protein efficiency for weight gain, and daily protein, fat, ash, and phosphorus depositions up to the estimated levels of 0.64, 0.66, 0.70, 0.70, 0.62, 0.62, 0.70, 0.71%, respectively. The phosphorus efficiency for weight gain worsened with the elevation of phosphorus levels. The recommendation of digestible phosphorus level in rations for tambaqui fingerlings to optimize weight gain and body phosphorus deposition is 0.71%, which corresponds to the estimated level of 1.04% total phosphorus.


2021 ◽  
Vol 5 (2) ◽  
pp. 95-98
Author(s):  
Saroj Regmi ◽  
Ishwar Chandra Prakash Tiwari ◽  
Naba Raj Devkota ◽  
Ramashish Sah ◽  
Ritesh Kumar Yadav ◽  
...  

A study was conducted at AFU livestock farm, Rampur, Chitwan in 2017-18 to determine the effect of dietary supplementation of garlic and ginger in different combination on feed intake, growth performance and economics by using commercial Cobb 500 broilers. A total of two hundred, 11-day-old chicks were allocated randomly to five different treatments. The experiment was designed in a Completely Randomized Design, each treatment with four replication and each replication had 10 birds. They were fed isoproteinous and isocaloric Basal diet (BD) and BD supplemented with four different levels of garlic and ginger such as T1 (BD only), T2 (BD + 1% garlic); T3 (BD + 1.0% ginger); T4 (BD + 0.5% garlic and 0.5% ginger) and T5 (BD + 1% garlic and 1% ginger). Weekly average body weight, weight gain, feed consumption, and feed efficiency were recorded up to sixth week of age. All data were statistical analyzed using Completely Randomized Design. The results showed that overall feed consumption was significantly (P≤0.05) higher for broiler fed diet supplemented with 0.5% garlic and 0.5% ginger (T4) followed by T5 (basal diet with 1% garlic and 1% ginger). On the other hand significantly higher (P≤0.01) cumulative weekly live body weight and body weight gain (g/bird) was found for the treatment with supplemented 1% garlic powder (T2), followed by broiler fed diet supplemented with 0.5% garlic and 0.5% ginger powder (T4). Similarly, feed conversion ratio was significantly (P≤0.01) better in broiler fed basal diet with supplementation of 1% garlic (T2) followed by basal diet with supplementation of 0.5% garlic and 0.5% ginger (T4). The maximum benefit was obtained from the broiler fed basal diet with supplementation of 1% garlic (T2). The findings revealed that broiler fed basal diet with supplementation of 1% garlic powder had helped as a growth promoter contributing to the better growth performance, feed efficiency and higher benefit: cost ratio. Thus, addition of 1% garlic powder can be safely recommended as a growth promoter in broilers.


2021 ◽  
Vol 890 (1) ◽  
pp. 012024
Author(s):  
N A Giri ◽  
N W W Astuti ◽  
Sudewi ◽  
M Marzuqi ◽  
Y N Asih

Abstract Fish hydrolysate contains hydrolyzed protein which comprised of peptides and free amino acids that are easily digested and absorbed by fish. This study aimed to determine the effectiveness of fish hydrolysate in improving feed efficiency and growth of coral trout. Five experimental diets were formulated to contain 47% protein and 12% lipid with different sources and levels of fish hydrolysate. Diet-1 and diet-2 were added with crustacean hydrolysate at 2% and 4% levels, respectively. Whereas diet-3 and diet-4 were supplemented with 2% and 4% of tuna hydrolysate. Diet-5 was formulated without fish hydrolysate supplementation (control). The diets were prepared as dry pellet with 3 mm diameter. Coral trout juveniles were produced in the hatchery of Institute for Mariculture Research and Fisheries Extension (IMRAFE), Gondol-Bali with the average body weight of 7.6 ± 0.9 g. The juveniles were maintained in 15 fiber tanks, 400L in volume, with a density of 40 fish/tank. The experiment was designed with a completely randomized design (CRD) with 5 treatments (different diets) and 3 replications for each treatment. The fish were fed the experimantal diets three times every day at satiation level for 84 days of feeding experiment. Results of the experiment showed that increased supplementation of fish hydrolysate from 2% to 4% in diet tended to improve fish growth performance. However, the best growth performance, in term of weight gain (WG) and specific growth rate (SGR), was obtained in fish fed diet supplemented with 4% crustacean hydrolysate. This growth performance was significantly higher than of the other treatments (P<0.05). The best feed efficiency (FE) and protein efficiency ratio (PER) were also obtained in fish fed diet supplemented with 4% crustacean hydrolysate. The results of the present study showed that supplementation of 4% crustacean hydrolysate in diet effectively improved the growth and feed efficiency of coral trout.


2017 ◽  
Vol 16 (2) ◽  
pp. 147
Author(s):  
Noviati Rohmatul Khasanah ◽  
Nur Bambang Priyo Utomo ◽  
Mia Setiawati ◽  
Munti Yuhana

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">The study was conducted to evaluate the performance of catfish fry grown using different dietary protein levels in the biofloc-base aquaculture system. Experiments using a completely randomized design, consisted of four treatments and three replications. The treatment consisted of:  protein 38% (A), 34% protein (B), 30% protein (C), and protein 26% (D). Catfish with initial weight of 0.83±0.01 g and length of 4.64±0.04 cm were cultured in 60 L tank with density of 90 fish each tank for 35 days. Inoculation of heterotrophic bacterial <em>Staphylococcus lentus</em> L1k were performed of 104 CFU/mL ratio of 15 was administrated once a day after two hours feeding in the morning. Feeding was conducted twice a day at 5% of the biomass weight. At the end of trial treatment (D) showed the highest survival rate (88.15±5.25%), the body lenght variance coefficient (9.58±0.51%) and protein retention (39.87±2.77%). Treatment (B) showed the highest growth rate (4.11±0.05%), total length (2.39±0.08 cm), and feed intake (318.76±4.63). Treatment (A) showed the highest feed efficiency (93.65±4.43%) while lowest lipid retention compared to others (22.20±1.20%.). Based on the results, it can be concluded that 34% protein feed (C) can replace 38% protein feed (B) catfish fry size 4−5 cm through biofloc-based system.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: biofloc, fry, growth, protein, <em>Staphylococcus lentus</em> L1k</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong><br /></strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Penelitian bertujuan mengevaluasi kinerja pertumbuhan benih lele dengan menggunakan kadar protein pakan yang berbeda pada sistem bioflok. Percobaan dilakukan menggunakan rancangan acak lengkap, terdiri atas empat perlakuan dan tiga ulangan. Perlakuan terdiri atas protein 38% (A), protein 34% (B), protein 30% (C), dan protein 26% (D), terdiri atas empat perlakuan dan tiga ulangan. Benih lele dengan berat rata-rata 0,83±0,01 g dan panjang rata-rata 4,64±0,04 cm dipelihara di akuarium berukuran 90×50×40 cm3 dengan padat tebar 90 ekor/akuarium selama 35 hari. Inokulasi bakteri heterotrof berupa <em>Staphylococcus lentus</em> L1k dilakukan pada setiap perlakuan kepadatan 104 CFU/mL. Penambahan sumber karbon berupa molase dengan C/N (Carbon/Nitrogen) rasio 15 diberikan satu kali sehari setelah dua jam pemberian pakan di pagi hari. Pakan diberikan dua kali sehari sebanyak 5% dari berat biomasa. Hasil penelitian menunjukkan pemberian kadar protein pakan berbeda memberikan hasil yang positif. Perlakuan (D) menunjukkan tingkat kelangsungan hidup (88,15±5,25%), koefisien keragaman panjang (9,58±0,51%) dan retensi protein (39,87±2,77%) terbaik. Perlakuan (B) menunjukkan laju pertumbuhan harian (4,11±0,05%), panjang total (2,39±0,08 cm), dan jumlah konsumsi pakan (318,76±4,63) tertinggi. Perlakuan (A) menunjukkan efisiensi pakan (93,65±4,43%) tertinggi namun menunjukkan retensi lemak (22,20±1,20%) terendah. Berdasarkan hasil penelitian disimpulkan bahwa pemberian pakan dengan kadar protein 34% mempunyai performa pertumbuhan yang sama dibandingkan protein 38% pada benih ikan lele berukuran 4−5 yang dipelihara menggunakan sistem budidaya bioflok.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: benih lele, protein, pertumbuhan, bioflok, <em>Staphylococcus lentus</em> L1k</p><p> </p>


2017 ◽  
Vol 16 (2) ◽  
pp. 136
Author(s):  
Noviati Rohmatul Khasanah ◽  
Nur Bambang Priyo Utomo ◽  
Mia Setiawati ◽  
Munti Yuhana

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">The study was conducted to evaluate the performance of catfish fry grown using different dietary protein levels in the biofloc-base aquaculture system. Experiments using a completely randomized design, consisted of four treatments and three replications. The treatment consisted of:  protein 38% (A), 34% protein (B), 30% protein (C), and protein 26% (D). Catfish with initial weight of 0.83±0.01 g and length of 4.64±0.04 cm were cultured in 60 L tank with density of 90 fish each tank for 35 days. Inoculation of heterotrophic bacterial <em>Staphylococcus lentus</em> L1k were performed of 104 CFU/mL ratio of 15 was administrated once a day after two hours feeding in the morning. Feeding was conducted twice a day at 5% of the biomass weight. At the end of trial treatment (D) showed the highest survival rate (88.15±5.25%), the body lenght variance coefficient (9.58±0.51%) and protein retention (39.87±2.77%). Treatment (B) showed the highest growth rate (4.11±0.05%), total length (2.39±0.08 cm), and feed intake (318.76±4.63). Treatment (A) showed the highest feed efficiency (93.65±4.43%) while lowest lipid retention compared to others (22.20±1.20%.). Based on the results, it can be concluded that 34% protein feed (C) can replace 38% protein feed (B) catfish fry size 4−5 cm through biofloc-based system.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: biofloc, fry, growth, protein, <em>Staphylococcus lentus</em> L1k</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong><br /></strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Penelitian bertujuan mengevaluasi kinerja pertumbuhan benih lele dengan menggunakan kadar protein pakan yang berbeda pada sistem bioflok. Percobaan dilakukan menggunakan rancangan acak lengkap, terdiri atas empat perlakuan dan tiga ulangan. Perlakuan terdiri atas protein 38% (A), protein 34% (B), protein 30% (C), dan protein 26% (D), terdiri atas empat perlakuan dan tiga ulangan. Benih lele dengan berat rata-rata 0,83±0,01 g dan panjang rata-rata 4,64±0,04 cm dipelihara di akuarium berukuran 90×50×40 cm3 dengan padat tebar 90 ekor/akuarium selama 35 hari. Inokulasi bakteri heterotrof berupa <em>Staphylococcus lentus</em> L1k dilakukan pada setiap perlakuan kepadatan 104 CFU/mL. Penambahan sumber karbon berupa molase dengan C/N (Carbon/Nitrogen) rasio 15 diberikan satu kali sehari setelah dua jam pemberian pakan di pagi hari. Pakan diberikan dua kali sehari sebanyak 5% dari berat biomasa. Hasil penelitian menunjukkan pemberian kadar protein pakan berbeda memberikan hasil yang positif. Perlakuan (D) menunjukkan tingkat kelangsungan hidup (88,15±5,25%), koefisien keragaman panjang (9,58±0,51%) dan retensi protein (39,87±2,77%) terbaik. Perlakuan (B) menunjukkan laju pertumbuhan harian (4,11±0,05%), panjang total (2,39±0,08 cm), dan jumlah konsumsi pakan (318,76±4,63) tertinggi. Perlakuan (A) menunjukkan efisiensi pakan (93,65±4,43%) tertinggi namun menunjukkan retensi lemak (22,20±1,20%) terendah. Berdasarkan hasil penelitian disimpulkan bahwa pemberian pakan dengan kadar protein 34% mempunyai performa pertumbuhan yang sama dibandingkan protein 38% pada benih ikan lele berukuran 4−5 yang dipelihara menggunakan sistem budidaya bioflok.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: benih lele, protein, pertumbuhan, bioflok, <em>Staphylococcus lentus</em> L1k</p><p> </p>


2021 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Geri Gunawan ◽  
Adelina Adelina ◽  
Indra Suharman

Asian redtail catfish (Hemibagrus nemurus) are fish that have high economic value. The Aimed of this study was to know of the effect the use of fermented Pistia stratiotes flour and to know the percentage of best P. stratiotes flour fermentation on the growth of Asian redtail catfish fingerling. The method in this study is a completely randomized design (CRD) with five treatments and three replications, the treatments are P0 (100% soybean flour, 0% fermented P. stratiotes flour), P1 (TK 95%, TKAT 5%), P2 (P2 TK 90%, TKAT 10%), P3 (TK 85%, TKAT 15%), and P4 (TK 80%, TKAT 20%). The fish used were 5.00 ± 1.00 cm long and weighed 1.50 ± 0.50 g, with a stocking density of 20 fish/m3. Fish are kept for 56% with a frequency of feeding three times a day. The results showed that the substitution of fermented P. stratiotes flour was able to effect the growth of Asian redtail catfish. The dosage of fermented P. stratiotes flour as much as 15% gives the best results on the growth of Asian redtail catfish, namely the specific growth rate (SGR), feed efficiency, and survival rate of 3.93%, 46.47% and 96.7%.


2021 ◽  
Vol 21 (1) ◽  
pp. 11-22
Author(s):  
Surianti Surianti ◽  
Fitratul Muaddama ◽  
Wahyudi Wahyudi ◽  
Sri Wahyuni Firman

This study aims to determine the effect of the dose of fermented rice bran using Lactobacillus sp. on growth performance and enzyme activity in tilapia. This research was conducted in Sidenreng Rappang Regency. The test animal used was tilapia larvae measuring 1.17 g. The maintenance container is a tarp pool with a size of 1 m x 1 m x 1 m filled with fresh water as much as 85 L. The study was designed using a completely randomized design consisting of four treatments and three replications, thus there were 12 experimental units. The treatment was determined based on the dose of fermented rice bran using Lactobacillus sp. which differ, namely 0, 10, 15 and 20%. The test feed was given to tilapia larvae, which were stocked with 20 fish per container for 60 days of rearing. The specific growth rate data of tilapia were analysed using analysis of variance (ANOVA) and enzyme activity was analysed descriptively based on the viability of tilapia. The results showed that tilapia fed with 20% fermented rice bran had a significant effect on the specific growth rate, protease and amylase enzyme activity. The optimal dosage of fermented rice bran using Lactobacillus sp. is 15-20%.


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