scholarly journals Potential of Parijoto (Medinilla speciosa) Fruits and Leaves in Male Fertility

2019 ◽  
Vol 20 (2) ◽  
pp. 81
Author(s):  
Dwi Wijayanti ◽  
Firgian Ardigurnita
Keyword(s):  
Key Words ◽  
Male Fertility ◽  
Beta Carotene ◽  
Secondary Compounds ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Qualitative And Quantitative ◽  
Herbal Plants ◽  
Livestock Productivity

The purpose of the study was to find out the potential of parijoto fruit and leaves qualitatively and quantitatively in male fertility. The content of fruits and parijoto were secondary compounds in the form of flavonoids, saponins, tannins, terpenoids, beta-carotene and antioxidants. Fertility is also needed in male animals. Decreased fertility in male cause a decrease in livestock productivity results in a decrease in gain from farmers. Handling of animals that occur after fertilization was usually carried out by injecting prostaglandin hormones. However, the relatively expensive price of hormones causes many farmers not to be able to buy. Another alternative that could cause a decrease in fertility was to use herbal plants, namely parijoto. The use of Parijoto plants was not certain which relevanted in fertility. Need to be tested first about phytochemical screening on parijoto leaves and fruit. The results of parijoto leaf and fruit analysis were qualitative and quantitative. The content of fruits and parijoto were secondary compounds in the form of flavonoids, saponins, tannins, terpenoids, beta-carotene and antioxidants. Parijoto fruits were founded flavonoids and saponins namely 3. 61% b/b and 3. 71% b/b. Total flavonoids and saponins in Parijoto leaves were 2.65% b/b and 4.19% b/b. Total antioxidants, total polyphenols, tannins in parijoto leaves and fruit respectively were 36.84 ppm; 3.95%; 3.52% and 20.06 ppm; 12.68%; 11.70%. The conclusion was that part of the parijoto fruit and leaf were the potential in male fertility. Key words:  Antioxidants, Fertility, Flavonoids, Males, Parijoto.

scholarly journals SKRINING FITOKIMIA DAN KANDUNGAN TOTAL FLAVANOID PADA BUAH Carica pubescens Lenne & K. Koch DI KAWASAN BROMO, CANGAR, DAN DATARAN TINGGI DIENG

el–Hayah ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 73 ◽  
Author(s):  
Eko Budi Minarno
Keyword(s):  
Positive Sample ◽  
Screening Tests ◽  
Total Flavonoids ◽  
Test Results ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Third ◽  
Fruit Samples ◽  
The Tropics ◽  
Carica Pubescens

<p class="Default"><em>Carica  pubescens Lenne &amp; K. Koch is one of the species in the tropics, which adapt to the plateau environment and low temperatures. In Indonesia, this plant is found at Cangar, Bromo, and Dieng Plateau. This study aims to determine the results of phytochemical screening and total flavonoids in fruit samples were taken from the third place. Qualitative and quantitative tests carried out in the Laboratory of Department of Biology and Chemistry, </em><em>Faculty of </em><em>Science and Technology</em><em>, </em><em>Maulana Malik Ibrahim State Islamic University, Malang</em><em>. Analysis of total flavonoids using a spectrophotometer at λ = 510 nm. The results of qualitative phytochemical screening tests on samples of C. pubescens fruits at Cangar, Bromo, and Dieng Plateau shows that the positive sample contains flavonoids, polyphenols, tannins, and triterpenoids. Quantitative test results show that the C. pubescens at Cangar contains  total flavonoids quercetin equivalent with value 800 mg / L, Bromo with value 816.65 mg / L, and Dieng Plateau with value 633.35 mg / L, respectively.</em></p>

scholarly journals Phytochemical, antioxidant and anticholinesterase profiles of Musanga cecropioïdes R. Br. (Urticaceae) from Côte d'Ivoire

2021 ◽  
Vol 16 (2) ◽  
pp. 094-102
Author(s):  
Djélé Alette Edwige Ziale ◽  
Kohué Christelle Chantal N’gaman-Kouassi ◽  
Fatou Diane Micheline Baguia-Broune ◽  
Janat Akhanovna Mamyrbekova-Békro ◽  
Yves-Alain Békro
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Phenolic Compounds ◽  
Quantitative Composition ◽  
Root Bark ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Tropical Plant ◽  
Anticholinesterase Properties

This study is devoted to Musanga cecropioides (Urticaceae), a tropical plant whose leaves and root bark are used in traditional medicine in the treatment of various pathologies. The objective of this work was to study qualitative and quantitative composition of phenolic compounds in extracts of the leaves (McF) and root bark (McR) Musanga cecropioides and to evaluate their antioxidant and anticholinesterase properties. Phytochemical screening using TLC showed that the extracts contain, along with phenolic compounds such as coumarins, flavonoids and tannins, other bioactive phytocompounds namely sterols, terpenes and alkaloids. Quantitative analysis of phenolic phytoconstituents by spectrophotometry showed that contents of total flavonoids and polyphenols in leaves (7.753% and 119.389 mg EAG / g, respectively) are higher than those in the root bark (1.41% and 105.944 mg EAG / g, respectively). The antioxidant activity of total and selective extracts evaluated by FRAP and DPPH methods was found to be significant compared to vitamin C. All the extracts of Musanga cecropioides showed good anticholinesterase activity with percentages of inhibition of the acetylcholinesterase (AChE) ranging from 51.952 to 63.589%.

scholarly journals The Qualitative And Quantitative Determination Of The Phenolic Compounds In Polygonum Convolvulus L. Species, Polygonaceae Family

2013 ◽  
Vol 59 (3) ◽  
pp. 162-164 ◽  
Author(s):  
O T Olaru ◽  
Adriana Iuliana Anghel ◽  
Viorica Istudor ◽  
Iulia Ioana Olaru
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Polyphenolic Compounds ◽  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Qualitative And Quantitative ◽  
Polygonum Convolvulus ◽  
Subalpine Zone ◽  
Layer Chromatography

Abstract Introduction: Polygonum convolvulus L. (black bindweed), syn. Fallopia convolvulus (L.) Á. Löve, Polygonaceae family is a plant from the spontaneous flora, spread from the plain zone up to the subalpine zone. The objectives of our researches are the qualitative and quantitative determination of polyphenolic compounds from Polygoni convolvuli herba and the choice of the adequate solvent for obtaining an active pharmacological extract. Method: The qualitative exam consisted of phytochemical screening and thin layer chromatography. The quantitative determination of the total polyphenols was made through the Folin-Ciocâlteu method. Results: The flavonoids, the anthocyanins, the tannins and the phenol carboxylic acids (phytochemical screening) were emphasized and the following compounds were identified: rutin, hyperoside, isoquercitroside, quercetin, myricetin, kaempferol and caffeic acid. Conclusions: In order to establish the technological lab process for obtaining an active pharmacological extract standardized in total polyphenols the adequate solvent is ethanol 50% (v/v).

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

Phytochemical Screening, Antimicrobial and Antioxidant Activities of Selected Mangrove Species

2020 ◽  
Vol 16 (2) ◽  
pp. 152-163 ◽  
Author(s):  
Nilesh L. Dahibhate ◽  
Utpal Roy ◽  
Kundan Kumar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Avicennia Marina ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Mangrove Species ◽  
Sonneratia Caseolaris ◽  
Bruguiera Gymnorhiza

Background: Mangrove plant extracts are used in folkloric medicine as aphrodisiac, sedative, antioxidant, antimicrobial and antimalarial. Screening for antimicrobial, phytochemical and antioxidant activities of fourteen mangroves plant species (Aegiceras corniculatum, Acanthus ilicifolius, Avicennia alba, Avicennia marina, Avicennia officinalis, Bruguiera cylindrica, Bruguiera gymnorhiza, Ceriops tagel, Excoecaria agallocha, Kandelia candel, Rhizophora apiculata, Rhizophora mucronata, Sonneratia alba, and Sonneratia caseolaris) in various solvents are reported in this work. Methods: The antimicrobial screening was carried out using agar well diffusion method. In this study, nine pathogenic strains were used, including three Gram-positive and six Gram-negative bacteria. Phytochemical screening, total flavonoids, total phenolic and antioxidant activity was tested by DPPH radical scavenging assay. Four phenolic compounds (gallic acid, vanillin, tannic acid and quercetin) were quantified by LC-MS/MS in selected mangrove species. Results: Antimicrobial screening showed Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa were most susceptible to ethyl acetate extract as compared to ethanol and methanol extract. Ethyl acetate extract of Avicennia marina and Bruguiera gymnorhiza showed strong antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa. Phytochemical analysis revealed the presence of saponins, phenolics, flavonoids, alkaloids, tannins, and terpenoids, which was found to be variable as per the solvent used for extraction. In addition, total phenolics and total flavonoids content with different solvents were found in the range of 11.08 to 196.76 mg GAE/g and 12.92 to 110.3 mg QE/g of extract respectively. Moreover, antioxidant capacities expressed in terms of IC50 (mg/mL) showed that methanol extract exhibited higher antioxidant capacity followed by ethanol extract. LC-MS/MS analysis showed gallic acid and tannic acid are present in higher concentration in Aegiceras corniculatum and Sonneratia caseolaris than other species. The vanillin and quercetin were found in the least concentration. Conclusion: Mangroves species are rich source of antioxidant, phenolics and antimicrobial compounds.

Isolation of Sinapic Acid from Habenaria intermedia D. Don: A New Chemical Marker for the Identification of Adulteration and Substitution

2020 ◽  
Vol 6 (4) ◽  
pp. 380-387
Author(s):  
Jaswinder Kaur Virk ◽  
Vikas Gupta ◽  
Mukesh Maithani ◽  
Ravindra K. Rawal ◽  
Sanjiv Kumar ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Sinapic Acid ◽  
Phytochemical Screening ◽  
Chemical Marker ◽  
Reference Compound ◽  
Crystalline Powder ◽  
Chemical Test ◽  
Herbal Plants ◽  
Single Compound ◽  
Layer Chromatography

Background: Vriddhi is one of the Rasayana herbs in Ayurveda broadly used in vitality, strengthening Ayurvedic formulations. To fulfill steeply increased demand and declined supply, tubers have been collected in destructive manner resulting in reduced plant population and pushing the plant in Red list of IUCN endangered species. However, manufacturers are using substitutes and other substandard drugs leading to adulteration which puts the importance of therapeutically rich herbal plants at stake. Lack of chemical markers is the main inability of regulatory authorities for not taking any action against this adulteration. Objective: Isolation of chemical marker of plant that can be used as a reference compound for identification of unauthorized substitution. Methods: Preliminary phytochemical screening of methanolic and toluene extract of H. intermedia D. Don was done using standard methods followed by column chromatography for the isolation of phytoconstituents. A total of 3004 fractions were collected with Thin Layer Chromatography (TLC) profiling and different fractions were pooled. A single compound was isolated and confirmed by chemical test, melting point, spectral analysis and compared with the literature. Results: Phytochemical screening of extracts shows the presence of alkaloids, carbohydrates, steroids, terpenoids, flavonoids, tannins and phenolics. A pure white crystalline powder was isolated by column chromatography which was characterized as 3,5-dimethoxy-4- hydroxycinnamic acid (Sinapic acid) with the help of IR and Mass spectroscopy. Conclusion: This is the first report of Sinapic acid as a novel compound from Vriddhi, Habenaria genus and Orchidaceae family. It can be used as a marker for the identification of unauthorized substitution and adulteration claiming the use of Vriddhi.

Phytopharmacognostical and Chromatographic Evaluation of Leaves of Launaea procumbens

2021 ◽  
pp. 4291-4299
Author(s):  
Brijesh Charadva ◽  
Urvashi Ghataliya ◽  
Pooja Meena ◽  
Krishna Karia ◽  
Tixa Lakhlani ◽  
...  
Keyword(s):  
Solvent System ◽  
Acid Anhydride ◽  
Phase System ◽  
Phytochemical Screening ◽  
Calcium Oxalate Crystal ◽  
Quantitative Microscopy ◽  
Qualitative And Quantitative ◽  
Anomocytic Stomata ◽  
Chloroform Methanol ◽  
Launaea Procumbens

Launaea procumbens leaves are galactagogue, diuretic, antifungal, anorexic, anti-arthritic and hepatoprotective according to Ayurvedic texts as well as modern research. Launaea species, particularly L. pinnatifida, is mentioned as one of the sources of Gojihva, regarding which there is controversy with respect to its true botanical identity. However, no detailed anatomical, phytochemical or chromatographic investigation is available for leaf of L. procumbens which can differentiate it from L. pinnatifida. The aim of this work is to develop standardization parameters of L. procumbens leaves by performing its pharmacognostical evaluation, preliminary phytochemical screening, HPTLC and GC-MS fingerprints. Pharmacognostic investigation of the leaves was performed by its morphological study, qualitative and quantitative microscopy as well as powder microscopy. Extraction of leaves was done by maceration using methanol. This extract was used for preliminary phytochemical screening and chemoprofiling by GC-MS, as well as for developing its HPTLC fingerprint. A mobile phase system was developed by pilot TLC, following which an HPTLC fingerprint was performed using the solvent system chloroform: methanol: ethyl acetate (3:7:6). Diagnostic microscopic characters identified in powder include unicellular covering trichome, xylem vessels, anomocytic stomata, and prisms of calcium oxalate crystal. Phytochemical screening revealed the presence of phytoconstituents classes like phenolics, anthraquinones, saponin glycosides, carbohydrates, sterols, triterpenoids and flavonoids. HPTLC fingerprinting detected 6 peaks with Rf 0.25, 0.37, 0.41, 0.50, 0.65, 0.78 at 254nm and 4 peaks with Rf 0.32, 0.37, 0.50, 0.66 at 366nm. GC-MS fingerprint revealed presence of propanoic acid anhydride, valeric anhydride, 2- Pyrrolidine acetic acid, phthalan, 5- (Hydroxy methyl)-2-(dimethoxy methyl) furan, vanillin, methyl β-l-Arabino pyranoside, 1,6-anhydro-β-D-Gluco pyranose and 6-Methyl-2-Heptanone, 6-(3,5-dimethyl-2-furanyl). Present study will be very useful for herbal industry in differentiating Launaea procumbens from other species of the genus Launaea, particularly L. pinnatifida (Gojihva) as well as for authentication, standardization and detection of adulteration in the leaf formulations of Launaea procumbens.

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

scholarly journals SCREENING OF PHYTOCHEMICALS AND QUANTITATIVE ESTIMATION OF TOTAL FLAVONOIDS AND PHENOLIC COMPOUNDS OF LAGASCEA MOLLIS CAV. (ASTERACEAE)

2014 ◽  
Vol 1 (2) ◽  
pp. 94-97
Author(s):  
Saradha M ◽  
Paulsamy S ◽  
Abinaya G
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Quantitative Estimation ◽  
Root Extract ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Plant Parts ◽  
Soxhlet Apparatus ◽  
Phytochemical Constituents ◽  
Leaves And Roots

Aim of the study was to analyze the phytochemical constituents and estimation of total flavonoids and phenolic compounds of leaves and roots of the medicinal plant Lagascea mollis. Methanol extract of dried leaves and roots of L. mollis was prepared by using soxhlet apparatus. The extract prepared was tested forpreliminary qualitative phytochemical screening, followed by the quantitative estimation of total flavonoids and phenols by spectroscopy. The selected plant parts were found to contain alkaloids, flavonoids and terpenoids in both parts and saponin is present only in leaf. In quantitative estimation the reports revealedthe presence 0.017 and 0.013 mgRE/g extract and 458.91 and 704.50 mgGAE/g extract total flavonoids and phenolic compounds in leaf and root extract respectively. These major phytoconstituents present in this species may be accounted as factors for the medicinal importance of L. mollis.

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