scholarly journals Green Synthesis of Copper Nanoparticles Using a Hydroalcoholic Extract of Moringa oleifera Leaves and Assessment of Their Antioxidant and Anti-microbial Activities

2019 ◽  
Author(s):  
Prince Edwin Das ◽  
Imad A. Abu-Yousef ◽  
Amin F. Majdalawieh ◽  
Srinivasan Narasimhan ◽  
Palmiro Poltronieri
Keyword(s):  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Moringa Oleifera ◽  
Color Change ◽  
Metallic Copper ◽  
Added Value ◽  
Total Phenolic ◽  
Copper Sulphate Solution ◽  
Hydroalcoholic Extract ◽  
Anti Fungal

The synthesis of metal nanoparticles using plant extracts is a very promising method in green synthesis. The medicinal value of Moringa oleifera leaves and the anti-microbial activity of metallic copper were combined in the present study to synthesize copper nanoparticles having a desirable added-value inorganic material. The use of a hydroalcoholic extract of M. oleifera leaves for the green synthesis of copper nanoparticles is an attractive method as it leads to the production of harmless chemicals and reduces waste. The total phenolic content in the M. oleifera leaves extract was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. The M. oleifera leaves extract was treated with a copper sulphate solution. A color change from brown to black indicates the formation of copper nanoparticles. Characterization of the synthesized copper nanoparticles was performed using UV-Vis spectrophotometer, FT-IR spectrometer, TEM, SEM, and XRD. The synthesized copper nanoparticles have an amorphous nature and particle size of 35.8-49.2 nm. We demonstrate that the M. oleifera leaves extract and the synthesized copper nanoparticles display considerable antioxidant activity. Moreover, the M. oleifera leaves extract and the synthesized copper nanoparticles exert potent anti-bacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis (MIC values for the extract: 500, 250, 250, and 250 μg/mL; MIC values for the cooper nanoparticles: 500, 500, 500, and 250 μg/mL, respectively). Similarly, the M. oleifera leaves extract and the synthesized copper nanoparticles exert relatively more potent anti-fungal activity against Aspergillus niger, Aspergillus flavus, Candida albicans, and Candida glabrata (MIC values for the extract: 62.5, 62.5, 125, and 250 μg/mL; MIC values for the cooper nanoparticles: 125, 125, 62.5, and 31.2 μg/mL, respectively). Our study reveals that the green synthesis of copper nanoparticles using a hydroalcoholic extract of M. oleifera leaves was successful. In addition, the synthesized copper nanoparticles can be potentially employed in the treatment of various microbial infections due to their potent antioxidant, anti-bacterial, and anti-fungal activities.

scholarly journals Green Synthesis of Encapsulated Copper Nanoparticles Using a Hydroalcoholic Extract of Moringa oleifera Leaves and Assessment of Their Antioxidant and Antimicrobial Activities

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 555 ◽  
Author(s):  
Prince Edwin Das ◽  
Imad A. Abu-Yousef ◽  
Amin F. Majdalawieh ◽  
Srinivasan Narasimhan ◽  
Palmiro Poltronieri
Keyword(s):  
Electron Microscopy ◽  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Moringa Oleifera ◽  
Metallic Copper ◽  
Total Phenolic ◽  
Copper Sulphate Solution ◽  
Hydroalcoholic Extract ◽  
Moringa Oleifera Leaves ◽  
Anti Fungal

The synthesis of metal nanoparticles using plant extracts is a very promising method in green synthesis. The medicinal value of Moringa oleifera leaves and the antimicrobial activity of metallic copper were combined in the present study to synthesize copper nanoparticles having a desirable added-value inorganic material. The use of a hydroalcoholic extract of M. oleifera leaves for the green synthesis of copper nanoparticles is an attractive method as it leads to the production of harmless chemicals and reduces waste. The total phenolic content in the M. oleifera leaves extract was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. The M. oleifera leaves extract was treated with a copper sulphate solution. A color change from brown to black indicates the formation of copper nanoparticles. Characterization of the synthesized copper nanoparticles was performed using ultraviolet-visible light (UV-Vis) spectrophotometry, Fourier-transform infrared (FTIR) spectrometry, high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The synthesized copper nanoparticles have an amorphous nature and particle size of 35.8-49.2 nm. We demonstrate that the M. oleifera leaves extract and the synthesized copper nanoparticles display considerable antioxidant activity. Moreover, the M. oleifera leaves extract and the synthesized copper nanoparticles exert considerable anti-bacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis (MIC values for the extract: 500, 250, 250, and 250 µg/mL; MIC values for the copper nanoparticles: 500, 500, 500, and 250 µg/mL, respectively). Similarly, the M. oleifera leaves extract and the synthesized copper nanoparticles exert relatively stronger anti-fungal activity against Aspergillus niger, Aspergillus flavus, Candida albicans, and Candida glabrata (MIC values for the extract: 62.5, 62.5, 125, and 250 µg/mL; MIC values for the copper nanoparticles: 125, 125, 62.5, and 31.2 µg/mL, respectively). Our study reveals that the green synthesis of copper nanoparticles using a hydroalcoholic extract of M. oleifera leaves was successful. In addition, the synthesized copper nanoparticles can be potentially employed in the treatment of various microbial infections due to their reported antioxidant, anti-bacterial, and anti-fungal activities.

scholarly journals Use of a Hydroalcoholic Extract of Moringa oleifera Leaves for the Green Synthesis of Bismuth Nanoparticles and Evaluation of Their Anti-Microbial and Antioxidant Activities

2019 ◽  
Author(s):  
Prince Edwin Das ◽  
Amin F. Majdalawieh ◽  
Imad A. Abu-Yousef ◽  
Srinivasan Narasimhan ◽  
Palmiro Poltronieri
Keyword(s):  
Green Synthesis ◽  
Enterococcus Faecalis ◽  
Moringa Oleifera ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Hydroalcoholic Extract ◽  
Bismuth Nanoparticles ◽  
Fungal Activity ◽  
Anti Fungal

The use of plant extracts in the synthesis of metal nanoparticles is a very attractive approach in the field of green synthesis. To benefit from the potential synergy between the biological activities of the Moringa oleifera leaves extract and metallic bismuth, our study aimed at synthesizing bismuth nanoparticles using a hydroalcoholic extract of M. oleifera leaves as a means of green synthesis that yields nontoxic products and reduces the production of wasteful material. To this end, the M. oleifera leaves extract was treated with a bismuth nitrate pentahydrate solution. A color change from light brown to dark brown indicates the synthesis of bismuth nanoparticles. The total phenolic content in the M. oleifera leaves extract used was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. Antioxidant property of MO synthesised bismuth Nanoparticles was evaluated and in line with the extract used in the synthesis of NPs. The physical properties of the synthesized bismuth nanoparticles were characterized using UV-Vis spectrophotometer, FT-IR spectrometer, TEM, SEM, and XRD. The synthesized bismuth nanoparticles have a size in the range of 40.4-57.8 nm with amorphous morphology. Using DPPH and phosphomolybdate assays, our findings revealed that the M. oleifera leaves extract and the synthesized bismuth nanoparticles possess antioxidant properties. Using resazurin microtiter assay, we also demonstrate that the M. oleifera leaves extract and the synthesized bismuth nanoparticles exert potent anti-bacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus faecalis, similarly to the inhibition exerted by Moringa extract, especially against Enterococcus faecalis (MIC values for the extract: 500, 250, 250, and 250 µg/mL; MIC values for the bismuth nanoparticles: 500, 500, 500, and 250 µg/mL, respectively). Similarly, the M. oleifera leaves extract and the synthesized bismuth nanoparticles display relatively stronger anti-fungal activity against Aspergillus niger, Aspergillus flavus, Candida albicans, and Candida glabrata (MIC values for the extract: 62.5, 62.5, 125, and 250 µg/mL; MIC values for the bismuth nanoparticles: 250, 250, 62.5, and 62.5 µg/mL, respectively). Thus, the hydroalcoholic extract of M. oleifera leaves was successfully used in the synthesis of bismuth nanoparticles, showing a positive antioxidant, anti-bacterial, and anti-fungal activity. Therefore, the synthesized bismuth nanoparticles can potentially be employed in the alleviation of symptoms associated with oxidative stress and in the topic treatment of Candida infections.

scholarly journals Use of A Hydroalcoholic Extract of Moringa oleifera Leaves for the Green Synthesis of Bismuth Nanoparticles and Evaluation of Their Anti-Microbial and Antioxidant Activities

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 876
Author(s):  
Prince Edwin Das ◽  
Amin F. Majdalawieh ◽  
Imad A. Abu-Yousef ◽  
Srinivasan Narasimhan ◽  
Palmiro Poltronieri
Keyword(s):  
Green Synthesis ◽  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Hydroalcoholic Extract ◽  
Bismuth Nanoparticles ◽  
Fungal Activity ◽  
Anti Fungal

The employment of plant extracts in the synthesis of metal nanoparticles is a very attractive approach in the field of green synthesis. To benefit from the potential synergy between the biological activities of the Moringa oleifera and metallic bismuth, our study aimed to achieve a green synthesis of phytochemical encapsulated bismuth nanoparticles using a hydroalcoholic extract of M. oleifera leaves. The total phenolic content in the M. oleifera leaves extract used was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. The physical properties of the synthesized bismuth nanoparticles were characterized using UV-Vis spectrophotometer, FT-IR spectrometer, TEM, SEM, and XRD. The size of the synthesized bismuth nanoparticles is in the range of 40.4–57.8 nm with amorphous morphology. Using DPPH and phosphomolybdate assays, our findings revealed that the M. oleifera leaves extract and the synthesized bismuth nanoparticles possess antioxidant properties. Using resazurin microtiter assay, we also demonstrate that the M. oleifera leaves extract and the synthesized bismuth nanoparticles exert potent anti-bacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis (estimated MIC values for the extract: 500, 250, 250, and 250 µg/mL; estimated MIC values for the bismuth nanoparticles: 500, 500, 500, and 250 µg/mL, respectively). Similarly, the M. oleifera leaves extract and the synthesized bismuth nanoparticles display relatively stronger anti-fungal activity against Aspergillus niger, Aspergillus flavus, Candida albicans, and Candida glabrata (estimated MIC values for the extract: 62.5, 62.5, 125, and 250 µg/mL; estimated MIC values for the bismuth nanoparticles: 250, 250, 62.5, and 62.5 µg/mL, respectively). Thus, green synthesis of bismuth nanoparticles using M. oleifera leaves extract was successful, showing a positive antioxidant, anti-bacterial, and anti-fungal activity. Therefore, the synthesized bismuth nanoparticles can potentially be employed in the alleviation of symptoms associated with oxidative stress and in the topic treatment of Candida infections.

scholarly journals Green Synthesis of Copper Nanoparticles Using Mitragyna Parvifolia Bark Extract and its Antimicrobial Study

2019 ◽  
pp. 26-33
Author(s):  
Shaileshkumar C Kotval ◽  
Kokila A Parmar
Keyword(s):  
Antimicrobial Activity ◽  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Color Change ◽  
Bark Extract ◽  
Gram Positive ◽  
Bending Vibrations ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Uv Visible

In this study, Mitragyna parvifolia plant bark an aqueous extract which provides cost-effective, eco-friendly process, less time consuming, an environmentally benign, easy and proficient way for the synthesis of copper nanoparticles. Mitragyna parvifolia plant bark was collected from virpur hills forest area. The Mitragyna parvifolia plant bark extract was prepared in de-ionised water and used for the green synthesis of copper nanoparticles. The color change of the solution dark brown from pale yellow colored, this confirms that there is a formation of copper nanoparticles. The green synthesised copper nanoparticles were characterized by UV-Visible spectroscopy, FT-IR, XRD, SEM, TEM and their antimicrobial activity was investigated. From UV-Visible spectrophotometer result was confirmed the reduction of copper sulphate to copper nanoparticles. FTIR analysis was confirmed the bending vibrations and stretching bonds present in the sample. Spherical shape was finding out by XRD and the size of the particle was analyzed with the help of Scanning Electron Microscopy. The antibacterial activity experiment against Escherichia coli gram-negative and Bacillus subtilis gram-positive bacteria by agar well method and the maximum zone of inhibition was higher in gram-positive bacteria compared to gram-negative bacteria. The green synthesised copper nanoparticles proved to be potential candidates for medical application antimicrobial activity is highly essential.

scholarly journals Biomediated Green Synthesis of Copper Nanoparticles using Piper nigrum Leaf Extract

2020 ◽  
Vol 6 (3) ◽  
pp. 908-910
Author(s):  
C. Sreeja ◽  
K. Annieta Philip ◽  
K. Shamil ◽  
O.P. Asraj ◽  
S. Sreeja
Keyword(s):  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Leaf Extract ◽  
Copper Chloride ◽  
Metallic Copper ◽  
Xrd Analysis ◽  
Absorption Peak ◽  
Piper Nigrum ◽  
Face Centered Cubic ◽  
Face Centered

In the present days the development of efficient green synthesis of metal nanoparticles has become a major focus of researchers. It was aimed in order to find a safe, economic and eco-friendly technique for the production of well characterized nanoparticles. The present study reports the green synthesis of copper nanoparticles using plant extract (pepper leaf extract) and a copper salt (copper chloride, CuCl2). Copper nanoparticles are produced by the reduction of CuCl2, while the aqueous leaf extract act as reducing agent. The formation of copper nanoparticles was recognized by the change of colour from blue to black. The synthesized nanoparticles were then characterized through EDAX, XRD, and UV-Vis spectrophotometry. EDAX confirmed the formation of copper nanoparticles. The UV-Vis spectrophotometer analysis confirmed the absorption peak of copper nanoparticles at wavelength of 500-700 nm. XRD analysis revealed three sharp peaks specifically referred to face centered cubic structure of metallic copper. This work was also carried out the optical studies of copper nanoparticles which were exposed to direct sunlight. This study reflected the effect of sunlight intensity on the absorption peak of copper nanoparticles.

Antibacterial mechanism of biogenic copper nanoparticles synthesized using Heliconia psittacorum leaf extract

2016 ◽  
Vol 5 (6) ◽  
Author(s):  
Kaushik Roy ◽  
Chandan K. Sarkar ◽  
Chandan K. Ghosh
Keyword(s):  
Green Synthesis ◽  
Copper Sulfate ◽  
Copper Nanoparticles ◽  
Leaf Extract ◽  
Metallic Copper ◽  
Sulfate Solution ◽  
Antibacterial Mechanism ◽  
The Novel ◽  
Copper Sulfate Solution

AbstractHere, we report on the novel green synthesis of metallic copper nanoparticles from copper sulfate solution by using the leaf extract of

scholarly journals Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract

2020 ◽  
Author(s):  
Srividya Parthasarathy ◽  
Sanjana Jayacumar ◽  
Sudestna Chakraborty ◽  
Prathyusha Soundararajan ◽  
Darshani Joshi ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Green Synthesis ◽  
Copper Nanoparticles ◽  
Leaf Extract ◽  
Cost Effective ◽  
Xrd Analysis ◽  
X Ray Diffraction ◽  
Copper Sulphate Solution ◽  
Synthesis Of Nanoparticles ◽  
Plectranthus Amboinicus

Abstract The field of nanotechnology is gaining interest among the researchers towards the eco-friendly way of synthesis of nanoparticles. In this project, green synthesis technique was employed to induce the synthesis of copper nanoparticles using Plectranthus amboinicus, i.e. Mexican mint, identified as Coleus amboinicus leaf extract. We report an eco-friendly synthesis of copper nanoparticle using Plectranthus amboinicus leaf extract, which is a simple and an ostentatiously rapid method which produces stable nanoparticles. The copper sulphate solution was naturally employed as a precursor for synthesizing the copper nanoparticles. The extract of the plant Plectranthus amboinicus was found to showcase excellent reducing and stabilizing properties. By using Ultraviolet-Visible spectroscopy, Zeta Potential, and X-Ray Diffraction (XRD) studies, it was confirmed that copper nanoparticles have been synthesized. The UV-Spectrometer analysis shows the characteristic peak indicating the synthesis of copper nanoparticles. The pattern of XRD analysis showed particle size of 16 - 25 nm and it reveals high crystallinity of the copper nanoparticles. Zeta potential was done to find the charge of the nanoparticles and size distribution which showed to have significant stability. This method proves to be cost-effective, can be performed at ease, and it’s also free of pollutants.

scholarly journals Degradation of Toxic Dye Using Phytomediated Copper Nanoparticles and Its Free-Radical Scavenging Potential and Antimicrobial Activity against Environmental Pathogens

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
S. Rajeshkumar ◽  
M. Vanaja ◽  
Arunachalam Kalirajan
Keyword(s):  
Copper Nanoparticles ◽  
Pathogenic Bacteria ◽  
High Efficiency ◽  
Color Change ◽  
Copper Ions ◽  
Dye Degradation ◽  
Radical Scavenging ◽  
Degradation Process ◽  
Methyl Red ◽  
Copper Sulphate Solution

The present investigation deals with the green synthesis of copper nanoparticles in an ecofriendly manner using leaf extract of Andrographis paniculata. Green-synthesized copper nanoparticles were studied for their antibacterial, antioxidant, and catalytic activity. The leaves were powdered and extracted with water and added to copper sulphate solution. The reduction of copper ions to nanoparticles was preliminarily identified by the color change of the reaction mixture. The synthesized nanoparticle was characterized by using a UV-Vis Spectrophotometer at a different wavelength with different time intervals. Functional groups available on the surface of the nanoparticle were identified by Fourier transform infrared spectroscopy (FTIR). Surface roughness was characterized by atomic force microscopy (AFM). X-ray diffraction (XRD) analysis showed six distinct intense peaks indicating the crystalline nature of synthesized copper nanoparticles (CuNPs). A scanning electron microscope (SEM) demonstrated polydispersed nanoparticles formed in the reaction process. The antibacterial activity of the nanoparticles was evaluated by an agar well diffusion assay against pathogenic bacteria. The antioxidant activity showed the excellent reduction of DPPH free radicals by nanoparticles. These results confirmed that copper nanoparticles serve as an alternative therapeutic agent over conventional drugs. Moreover, copper nanoparticles were also used to study the effect on the dye degradation process of methyl red and eosin dyes. Copper nanoparticles effectively remove the dyes with high efficiency up to 92% and 95% of methyl red and eosin dye, respectively.

Green Synthesis of Copper Oxide Nanoparticles using Cucumis sativus (cucumber) Extracts and their Bio-physical and Biochemical Characterization for Cosmetic and Dermatologic Applications

2020 ◽  
Vol 20 ◽  
Author(s):  
Monika Vats ◽  
Shruti Bhardwaj ◽  
Arvind Chhabra
Keyword(s):  
Copper Oxide ◽  
Green Synthesis ◽  
Biochemical Characterization ◽  
Chemical Characterization ◽  
Ultra Violet ◽  
Skin Penetration ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Ultra Violet Radiation ◽  
Anti Fungal

Background & Objective: Nanoparticles are used in cosmetic and dermatologic products, due to better skin penetration properties. Incorporation of natural products exhibiting medicinal properties in nano-preparations could significantly improve efficacy of these products and improve the quality of life without the side effects of synthetic formulations. Methods: We here report green synthesis of Copper Oxide nanoparticles, using Cucumber extract, and their detailed biophysical and bio-chemical characterization. Results: These Copper Oxide-Cucumber nanoparticles exhibit significant anti-bacterial and anti-fungal properties, Ultra Violet-radiation protection ability and reactive-oxygen species inhibition properties. Importantly, these nanoparticles do not exhibit significant cellular toxicity and, when incorporated in skin cream, exhibit skin rejuvenating properties. Conclusion: Our findings have implications for nanoparticle-based cosmetics and dermatologic applications.

Green Synthesis and Spectroscopic Studies of Ag-rGO Nanocomposites for Highly Selective Mercury (II) Sensing

2018 ◽  
Vol 9 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Shubhangi J. Mane-Gavade ◽  
Sandip R. Sabale ◽  
Xiao-Ying Yu ◽  
Gurunath H. Nikam ◽  
Bhaskar V. Tamhankar
Keyword(s):  
Green Synthesis ◽  
Color Change ◽  
Limit Of Detection ◽  
Aqueous Media ◽  
Suitable Condition ◽  
Cost Effective ◽  
Spectroscopic Studies ◽  
Calibration Plot ◽  
First Time

Introduction: Herein we report the green synthesis and characterization of silverreduced graphene oxide nanocomposites (Ag-rGO) using Acacia nilotica gum for the first time. Experimental: We demonstrate the Hg2+ ions sensing ability of the Ag-rGO nanocomposites form aqueous medium. The developed colorimetric sensor method is simple, fast and selective for the detection of Hg2+ ions in aqueous media in presence of other associated ions. A significant color change was noticed with naked eye upon Hg2+ addition. The color change was not observed for cations including Sr2+, Ni2+, Cd2+, Pb2+, Mg2+, Ca2+, Fe2+, Ba2+ and Mn2+indicating that only Hg2+ shows a strong interaction with Ag-rGO nanocomposites. Under the most suitable condition, the calibration plot (A0-A) against concentration of Hg2+ was linear in the range of 0.1-1.0 ppm with a correlation coefficient (R2) value 0.9998. Results & Conclusion The concentration of Hg2+ was quantitatively determined with the Limit of Detection (LOD) of 0.85 ppm. Also, this method shows excellent selectivity towards Hg2+ over nine other cations tested. Moreover, the method offers a new cost effective, rapid and simple approach for the detection of Hg2+ in water samples.

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