scholarly journals Histopathologic features of male domestic cat (Felis domestica) testes induced by bisphenol-A orally

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Fachira Ulfa Makmur ◽  
Fika Yuliza Purba ◽  
Dwi Kesuma Sari
Keyword(s):  
Bisphenol A ◽  
Reproductive System ◽  
Leydig Cells ◽  
Domestic Cat ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Interstitial Tissue ◽  
Group 2 ◽  
Testicular Histology ◽  
Group 1

Infertility in cat becomes a serious problem in pet community since many compounds of infertility-caused can be found widely in the environment. One of the compounds causing infertility in mammals is Bisphenol-A (BPA), which is known as an estrogenic compound. The purpose of this research is to observe the changes in testicular histology of male domestic cat (Felis domestica) induced by BPA orally. Sample used in this study were 24 male domestic cats divided into 4 groups, namely the group 1 as control, group 2, 3 and 4 were treated with BPA about 5, 10 and 100 mg/kg bodyweight, respectively for 5 days. The results showed that the estrogenic effect of BPA affects the reproductive system of the cat. The effects of BPA in cat included decrease in the amounts of spermatozoa, vacuolization of seminiferous tubules cells, degeneration of Leydig cells, degeneration of tubular epithelial cells,  irregular forms of the germ, interstitial tissue hemorrhage, debris cell filled the lumen of seminiferous tubules. These results suggested that BPA can be harmful to the reproductive system of the cat

scholarly journals Effect of immunological castration of male pigs on morphological and functional con-dition of the testicles

2021 ◽  
Vol 12 (1) ◽  
pp. 20-26
Author(s):  
V. V. Samoilіuk ◽  
M. S. Koziy ◽  
D. D. Bilyi ◽  
S. M. Maslikov ◽  
Т. L. Spitsina ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Leydig Cells ◽  
Functional Condition ◽  
Time Shift ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Interstitial Tissue ◽  
Livestock Products ◽  
Degree Of Differentiation ◽  
Immunological Castration

Changes in the priorities of the treatment of animals in the conditions of intense technology of production of livestock products are based on the necessity of wellbeing of animals. Therefore, there is a need of search for and broad introduction of generally accepted alternatives to surgery, which would use modern means of castration, particularly immunocastration. The study presents morpho-functional substantiation of practicability of using immunological castration in the conditions of industrial production of pork. At the same time, we studied immunological castration using Improvak on the morphological and physiological condition of the testicles of male pigs. The testosterone level was determined using radioimmunologic method after 2, 4, 6, 8 weeks of immunological and surgical castrations, and also in intact boars of the control group. During the slaughter, we selected biopates of the testicles in immunological castrates and pigs of the control group for histological examination. The testosterone level 2 weeks after castration was the lowest in the animals castrated using Improvak. This indicator gradually increased, and after 8 weeks was higher than in the surgically castrated pigs. In the latter, the level of testosterone gradually decreased for 8 weeks, and did not significantly change in non-castrated pigs. The last stages of spermatogenesis in immunocastrates were inhibited after the second vaccination. As a result of immunological castration, the interstitial tissue of the testicle underwent changes. Between the tubules, a spreading of the loose connective tissue was observed. Leydig cells lost hyper chromaticity of the cytoplasm and typical polygonal profile, and their functional potential decreased. This fact was confirmed by the changes in the Hertwig’s ratio. In particular, we observed decrease in the value of the nuclear-cytoplasmic ratio. There were also a time shift of mitotic cycle, low degree of differentiation of spermatogonia and rupture of the course of the subsequent stages of spermatogenesis. However, there occurred multiplication of primary spermatogonia, single cellular divisions, and in the ductus deferentes, there could be found single spermatids. Some of them formed specific cellular groups of rounded and elliptic shapes in the seminiferous tubules. These structures were absent in the testicles of the control animals. Microstructural changes in the swine after injecting Improvak were characterized by deficiency of Leydig cells, indicating absence of the normal hormonal background, as confirmed by the results of the study of testosterone level. The epithelium-spermatogenic layer was underdeveloped, and the lumens of the tubules were in some places filled with generations of spermatocytes. In some places, meiosis was observed, which also indicates insignificant functioning of the testicles. Vaccination with Improvak caused atrophy of the testicles in swine and decrease in their functional condition, allowing it to be recommended it for broader application as an alternative to surgical castration.

scholarly journals Histological and Weight Changes in the Testes of Plasmodium Berghei- Infected Swiss Mice Treated with Chloroquine

2021 ◽  
Vol 4 (2) ◽  
pp. 39-43
Author(s):  
N.S. Etukudoh ◽  
A.B. Deko ◽  
Uchejeso Obeta ◽  
S.K. Oyero ◽  
O.R. Ejinaka ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Weight Changes ◽  
Swiss Mice ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Chloroquine has gained great emphasis in the treatment of malaria. This study sought experimentally to determine the histomorphological and weight changes in the testes of male mice infected with Plasmodium berghei and treated with chloroquine. The study used 30 Swiss mice divided into five groups. Group 1 is Control that was not infected with Plasmodium berghei and not treated with chloroquine as control, Group 2 is Plasmodium (Plasmodiul berghei) Infected animals but not treated, Group 3 is Plasmodium Infected animals + Chloroquine (5mg/kg), Group 4 is Plasmodium Infected animals + Chloroquine (10mg/kg) and Group 5 is Plasmodium Infected animals + Chloroquine (15mg/kg). The mice were treated for 7 days after parasitaemia was confirmed and the Group 2-5 testes studied with reference to Group 1. The results showed that the Group 2 showed a little distortion, difference in spermatogenic activities and increased cellular activities; Group 3 showed large, convoluted tubules, moderate number of spermatids and large interstitial spaces, Group 4 showed Large seminiferous tubules, large spermatids, increased distortion and group 5 showed shrinking of seminiferous tubules, degeneration of interstitial cells of Leydig cells and Sertolic cells with spermatids. Groups 1-5 showed no significant effect in in body weights and testes weights of Swiss mice. Plasmodium berghei (malaria) and chloroquine have effects on histomorphological structures of Swiss mice testes but not on their teste’s weights. The testicular section from Swiss mice infected with malaria and treated at various doses when compared with the Control (Group 1) showed some moderate distortion in some structures like seminiferous tubules, connective tissues between the tubules, lumen and interstitial spaces. It can be deducted that Plasmodium berghei which caused parasitaemia in mice could cause a little tissue effect on mice if not treated.

scholarly journals Alcohol Consumption and Its Effect on Testicular Structure and on Sperm Count and Motility in Parent Mice and Their Offspring

2013 ◽  
Vol 12 (1) ◽  
Author(s):  
Cinaria T. Albadri
Keyword(s):  
Alcohol Consumption ◽  
Sperm Count ◽  
Male Offspring ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Ethanol Treatment ◽  
Male Mice ◽  
Female Mice ◽  
Group 2 ◽  
Group 1

Introduction: The goal of the present study was to examine the effect of alcohol consumption on sperm count and motility and the morphological changes in the seminiferous tubules of parent mice and their offspring. Methods: Animals were divided into two groups, Group 1 (alcohol group) of twelve male and twelve female mice, were given a daily dose of (3 g/kg body weight as 25%, v/v) ethanol by gastric gavage for four and eight weeks. Group 2 (control group) also of twelve male and twelve female mice; received normal access of food and water. After four weeks of treatment, the males and females in each group were allowed to mate, and ethanol treatment continued for up to another four weeks. Twelve male offspring from group 1 and twelve male offspring from group 2 were selected randomly and allowed to become mature. Male parent mice were killed at the 4th and 8th weeks of treatment, and their male offsprings were killed when they reached maturity age. Results: Physiological examination of the sperm solution showed that there was a significant decrease in sperm count and motility after 4 and 8 weeks of ethanol treatment in parent male mice, but this decrease was not significant in their adult offspring. Furthermore, histological investigations indicated testicular lesions in the parent male mice and their adult male offspring. Conclusion: Alcohol abuse has deleterious effects on the testes structure and on the sperm count and motility of the epididymal spermatozoa of both parent mice and their offspring.

scholarly journals Study on the effect of maternal administration of oxaliplatin on offspring testes using unbiased design-based stereology

2021 ◽  
Vol 38 (2) ◽  
pp. 99-106
Author(s):  
Javad SADEGHINEZHAD ◽  
Moslem DAHMARDEH ◽  
Zahra TOOTIAN ◽  
Hadis BOJARZADEH ◽  
Fatemeh YARMAHMOUDI
Keyword(s):  
Surface Area ◽  
Male Offspring ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Interstitial Tissue ◽  
Left Testis ◽  
Cell Divisions ◽  
Pregnancy And Lactation ◽  
Maternal Administration ◽  
Group 1

Oxaliplatin (Ox) is widely used for the treatment of various tumors. Since Ox prevents DNA replication and transcription, it may affect organs with rapid cell divisions such as the testes. Although its use during pregnancy has been reported, no information regarding its effects on the testes of the offspring is not available yet. Thirty-two mice were randomly divided into four groups. The control group (1) was administered intraperitoneally 0.2 ml of saline three times a week for the 21 days of pre-pregnancy, pregnancy and lactation. Experimental groups 2, 3 and 4 received 3 mg/kg of oxaliplatin three times a week for 21 days during pre-pregnancy, pregnancy and lactation, respectively. The left testis was removed from male offspring 30 and 60 days after birth. The volume of the testes, seminiferous tubules and interstitial tissue, the surface area and height of the seminiferous epithelium, as well as the length and diameter of seminiferous tubules, were analyzed by means of stereology. Results showed a decrease in the evaluated parameters in experimental groups, in comparison with the control group. Due to the ameliorating effect of Ox on offspring testes, cautiousness is needed during maternal administration in order to preserve the fertility of male offspring.

scholarly journals The effects of electromagnetic waves emitted by the cell phones on the testicular tissue

2014 ◽  
Vol 86 (4) ◽  
pp. 274
Author(s):  
Muhammet Ihsan Karaman ◽  
Ali Murat Gökçe ◽  
Orhan Koca ◽  
Bilal Karaman ◽  
Metin Ishak Öztürk ◽  
...  
Keyword(s):  
Electromagnetic Waves ◽  
Cell Phones ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Negative Effects ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objectives: Various risks have emerged in parallel to the rapidly increasing use of cell phones. Herein we studied the effects of cell phone emitted electromagnetic waves (EMW) on rat testes. Material and Methods: Twenty one adult male Albino rats were grouped into 3 groups each consisting of 7 rats. The first group was exposed to EMW on talk mode for 8 hours per day for 20 days and then their testes were extracted. The testes of the second group were extracted after 20 days of whole day EMW exposure. The third group was the control group. For the statistical analysis Mann- Whitney U analysis was performed. Results: At light microscopic examination of the testicular tissue, the existence of a high number of immature cells in the lumen of the seminiferous tubule in addition to the normal seminiferous tubules, besides irregular tubules with a reduction in the spermatogenic cell lines and tubules without lumen were observed in groups 1 and 2. Histopathological alterations were scored as 0 = none, 1 = low, 2 = medium, 3 = serious. The average scores of the three groups were found to be 4.25 ± 1.5 for the group 1, 4.33 ± 3.9 for the group 2 and 0.37 ± 1.1 for the group 3 respectively. As a result of the statistical evaluation, group 1 and group 2 had significantly higher scores than the control group (p = 0.001). Conclusion: Infertility is one of the current problems of today due to a rapid increase in its incidence and cost. The negative effects of the EMWs on the testis should be taken into account and the necessary measures should be taken for prevention.

The state of the testis in the boys presenting with cryptorchism during the mini-pubertal period

2014 ◽  
Vol 60 (2) ◽  
pp. 4-7
Author(s):  
N Yu Raigorodskaya ◽  
N V Bolotova ◽  
D A Morozov ◽  
N B Zakharova
Keyword(s):  
Clinical Examination ◽  
Reproductive System ◽  
Functional Condition ◽  
Inhibin B ◽  
Control Group ◽  
Testicular Function ◽  
Serum Gonadotropin ◽  
Group 2 ◽  
Hormonal Analysis ◽  
Group 1

The period of transitory postnatal activation of the hypotalamo-pitutary-gonadal axis (mini-puberty) plays an important role in the testicular maturation and the development of the reproductive system. The objective of the present study was to evaluate the functional condition of the testicles in the boys at the age of 2-3 months born with cryptorchism. A total of 32 boys presenting with inguinal retention of the testes (group 1), 10 patients with unilateral abdominal criptorchism (group 2), and 40 healthy boys ages 2-3 month (control group) were available for the clinical examination and hormonal analysis. The methods employed in the study included orchidometry and genitometry, meagurement of serum gonadotropin, testosterone, and inhibin B levels by immunoenzyme assays. The results investigations made it possible to diagnose gonadal pathology in 80% of the examinad children presenting with unilateral abdominal cryptorchism and subclinical disorders of the testicular function in 40% of the patients with inguinal retention of a testicle.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

Influence of Removable Denture Cleaning Agents on Adhesion of Oral Pathogenic Microflora In Vitro: A Randomized Controlled Trial

2021 ◽  
Vol 14 (1) ◽  
pp. 656-664
Author(s):  
I.R. Volchkova ◽  
A.V. Yumashev ◽  
V.V. Borisov ◽  
V.I. Doroshina ◽  
E.A. Kristal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Test Group ◽  
Control Group ◽  
Removable Dental Prostheses ◽  
Removable Denture ◽  
Group 2 ◽  
Adhesion Index ◽  
Group 1

Introduction: Removable dentures are used by 20% of the population. These may be accompanied by denture stomatitis in 15-70% of patients. The choice of the optimal cleansing agent for removable dental prostheses is of high significance. Aim: The aim of our research was to study the influence of removable denture cleansing products on the adhesion of microorganisms and yeast. Materials and Methods: We manufactured 144 specimens of standardized round shape with a diameter of 10 mm from 4 types of modern polymeric materials used by prosthetic dentistry to produce removable dentures, 12 specimens of each material were placed into suspensions of bacterial cultures of Staphylococcus aureus, Escherichia coli, Candida albicans, then into “ClearaSept” (Test group 1), “Рrotefix active cleanser” (Test group 2), saline solution (Control group), followed by nutrient media. The adhesion index was calculated and analyzed. Results: There was no reliable lowering of adhesion index of Staphylococcus Aureus to all materials detected in Test group 1 (U=6, p>0.05 for Bio XS; U=8, p>0.05 for Dental D, Denotokeep Peek, Vertex Rapid Simplified). In Test group 2, the adhesion index of Staphylococcus Aureus reliably decreased to all materials compared to the Control group (U=0, p≤0.01). The adhesion index of Candida albicans and Escherichia coli to all materials in Test group 1 had a minor to moderate reliable reduction compared to the Control group (U=0, p≤0.01). Test group 2 showed a significant reliable decrease in Candida albicans and Escherichia coli adhesion index to all materials in comparison with the Control group (U=0, p≤0.01). Conclusion: The research showed an unreliable or minor and moderate reliable decrease in microorganisms adhesion index depending on the microorganism species after treatment of denture material specimens by antibacterial soap “ClearaSept” and a reliable significant decrease in microbial and yeast adhesion after application of Protefix active cleaner solution, which demonstrates a more significant antimicrobial effect in comparison to “ClearaSept” against Staphylococcus aureus, Escherichia coli, and Candida albicans.

Dopamine-1 receptor stimulation impairs intestinal oxygen utilization during critical hypoperfusion

2003 ◽  
Vol 284 (2) ◽  
pp. H668-H675 ◽  
Author(s):  
Jorge A. Guzman ◽  
Ariosto E. Rosado ◽  
James A. Kruse
Keyword(s):  
Blood Flow ◽  
Mucosal Blood Flow ◽  
Control Group ◽  
Oxygen Utilization ◽  
Hemorrhage Control ◽  
Receptor Stimulation ◽  
Anesthetized Dogs ◽  
Group 2 ◽  
Increased Susceptibility ◽  
Group 1

Effects of a dopamine-1 (DA-1) receptor agonist on systemic and intestinal oxygen delivery (D˙o 2)-uptake relationships were studied in anesthetized dogs during sequential hemorrhage. Control ( group 1) and experimental animals ( group 2) were treated similarly except for the addition of fenoldopam (1.0 μg · kg−1 · min−1) in group 2. Both groups had comparable systemic criticalD˙o 2(D˙o 2crit), but animals in group 2 had a higher gut D˙o 2crit(1.12 ± 1.13 vs. 0.80 ± 0.09 ml · kg−1 · min−1, P < 0.05). At the mucosal level, a clear biphasic delivery-uptake relationship was not observed in group 1; thus oxygen consumption by the mucosa may be supply dependent under physiological conditions. Group 2 demonstrated higher peak mucosal blood flow and lack of supply dependency at higher mucosalD˙o 2 levels. Fenoldopam resulted in a more conspicuous biphasic relationship at the mucosa and a rightward shift of overall splanchnic D˙o 2crit despite increased splanchnic blood flow. These findings suggest that DA-1 receptor stimulation results in increased gut perfusion heterogeneity and maldistribution of perfusion, resulting in increased susceptibility to ischemia.

Biomarkers of vascular wall damage in patients with cerebrovascular diseases and type 2 diabetes mellitus

2021 ◽  
Author(s):  
М.А. Быковская ◽  
А.А. Раскуражев ◽  
А.А. Шабалина ◽  
К.В. Антонова ◽  
М.М. Танашян
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Asymmetric Dimethylarginine ◽  
Vascular Wall ◽  
Cerebrovascular Diseases ◽  
Control Group ◽  
Normal Body Mass Index ◽  
Group 2 ◽  
Group 1

Введение. Сосудистые осложнения сахарного диабета (СД) являются одной из ведущих причин повышения смертности пациентов трудоспособного возраста. Предполагается, что индуцированный гипергликемией окислительный стресс и нарушение антиоксидантной защиты играют роль в патологическом механизме повреждения сосудов, частично за счет влияния оксида азота (NО). Цель исследования: уточнение взаимосвязей в системах асимметричного диметиларгинина (АДМА) и NO у пациентов с цереброваскулярными заболеваниями (ЦВЗ) на фоне СД 2-го типа (СД-2). Материалы и методы. Обследованы 72 пациента с ЦВЗ со стенозирующим поражением внутренней сонной артерии вне острого периода: группу 1 составили 39 пациентов (18 мужчин и 21 женщина) с СД-2 в возрасте 65 [58; 72] лет; в группу 2 вошли 33 больных (15 мужчин и 18 женщин) без СД-2 в возрасте 66 [56; 74] лет. Контрольную группу составили 30 добровольцев (16 мужчин и 14 женщин) без проявлений церебральной ишемии и нарушений углеводного обмена, с нормальными значениями индекса массы тела, некурящие, в возрасте 62 [50; 66] лет. Проводилось клиническое обследование, нейро- и ангиовизуализационное исследование, спектр биохимических исследований крови, в том числе определение содержания АДМА и показателей системы NO. Результаты. В группе 1 содержание нитрата, нитрита и NO составило 62,1 [56; 68] мкмоль/л, 48,5 [26; 52] мкмоль/л и 13,6 [9; 23] мкмоль/л соответственно, что достоверно отличалось от значений этих показателей в группе 2 — 58,3 [45; 64] мкмоль/л, 39,6 [26,0; 42,3] мкмоль/л и 18,7 [16,1; 24,7] мкмоль/л соответственно. Отмечен также более высокий уровень AДМА в крови у пациентов с ЦВЗ в сочетании с СД-2 — 0,42 [0,21; 0,53] ммоль/л. Заключение. Обнаружена взаимосвязь между уровнями AДМА и NO при ЦВЗ на фоне СД-2. Это требует продолжения исследований биомаркеров повреждения сосудистой стенки для определения их места в патогенезе ишемических церебральных осложнений СД-2. Background. Vascular complications of diabetes mellitus (DM) are one of the leading causes of increased mortality in patients of employable age. Hyperglycemia-induced oxidative stress and impaired antioxidant protection have been suggested to play a role in the pathological mechanism of vascular damage, in part due to the effects of nitric oxide (NO). Objectives: clarification of relationships in the systems of asymmetric dimethylarginine (ADMA) and NO in patients with cerebrovascular diseases (CVD) and type 2 diabetes (DM-2). Patients/Methods. We examined 72 CVD patients with stenosing lesions of the internal carotid artery outside the acute period: group 1 consisted of 39 patients (18 men and 21 women; 65 [58; 72] years old) with DM-2; group 2 consisted of 33 patients (15 men and 18 women; 66 [56; 74] years old) without DM-2. The control group consisted of 30 volunteers (16 men and 14 women; 62 [50; 66] years old) without manifestations of cerebral ischemia and carbohydrate metabolism disorders, with normal body mass index, non-smokers. A clinical examination, neuro- and angio-imaging study, a spectrum of biochemical blood tests, including the concentration of asymmetric dimethylarginine (ADMA) and indicators of NO system were carried out. Results. In group 1, the content of nitrate, nitrite and NO was 62.1 [56; 68] μmol/l, 48.5 [26; 52] μmol/l and 13.6 [9; 23] μmol/l, respectively, that significantly differed from the content of these parameters in group 2 — 58.3 [45; 64] μmol/l, 39.6 [26.0; 42.3] μmol/l and 18.7 [16.1; 24.7] μmol/l, respectively. Noted also a higher blood level of ADMA in patients with CVD combined with DM-2 — 0.42 [0.21; 0.53] mmol/l. Conclusions. A relationship was found between ADMA and NO levels in CVD patients with DM-2. This requires further studies of biomarkers of vascular wall damage to determine their place in the pathogenesis of ischemic cerebral complications of DM-2.

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