6. The Effect of Celery Leaves Infusa (Apium graveolens L) on Reducing Level of Blood Glucose on Rat (Rattus norvegicus) Induced by Alloxan

This study aims to investigate the effect of celery leaf infusa on decreasing level of blood glucose in rat (Rattus norvegicus). Twenty five rats were divided into 5 groups namely first treatment group (P1) as negative control. Second treatment group (P2) as positive control, rats were induced with alloxan. Third treatment group (P3) rats were induced with alloxan and given 5% celery leaf infusa. Fourth treatment group (P4) rats were induced with alloxan and given 10% celery leaf infusa. Fifth treatment group (P5) rats were induced alloxan and given 15% celery leaf infusa for 14 days. The level of blood glucose of the rat was determined before treatment, after given aloksan and after given celery leaf infusa. Data were analyzed using Analysis of Varians (ANOVA). Results showed that administration of celery leaf infusa for 14 days, show significant effect to decreasing level of glucose in rat (P0.01). It can be concluded that the administration of celery leaf infusa concentrated 5%, 10% and 15% for 14 days show significant effect to decreased level of glucose in rats.

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PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

Biopendix: Jurnal Biologi, Pendidikan dan Terapan ◽

10.30598/biopendixvol1issue2page198-208 ◽

2015 ◽

Vol 1 (2) ◽

pp. 198-208

Author(s):

Nur Fitri

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Healing Process ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

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Liver Histopathological Change and Malondialdehyde Level of Rattus Norvegicus on Administration of Curcuma Zedoaria and Paracetamol Toxic Dose

Indonesian Journal of Medical Laboratory Science and Technology ◽

10.33086/ijmlst.v3i1.1927 ◽

2021 ◽

Vol 3 (1) ◽

pp. 38-46

Author(s):

Putu Oky Ari Tania ◽

Puja Ayu Misuari ◽

Satya Yudhayana ◽

Ketut Ayesha Edelwise Prayoga

Keyword(s):

Body Weight ◽

Free Radicals ◽

Treatment Group ◽

Rattus Norvegicus ◽

Histopathological Change ◽

Positive Control ◽

Negative Control ◽

Histological Structure ◽

Curcuma Zedoaria ◽

Toxic Dose

High doses of paracetamol create necrosis in the liver and produce free radicals. When liver function decreased in a long time, it will lead to severe liver damage and it will be irreversible. Rhizome of Curcuma zedoaria has the potential effect as an antioxidant, it is assumed that its properties inhibit the formation of free radicals which formed from toxic doses of paracetamol. The aim of this study was to examine the histological structure of the liver and to determine MDA levels in the administration of C. zedoaria toxic dose and paracetamol on the Rattus norvegicus. The study was used twenty-four rats divided into four groups (positive control: CMC 0.5%; negative control: paracetamol 1.35g/kg body weight; treatment group 1 (T1): C. zedoaria 105 mg/200g and paracetamol 1.35g/kg Body weight 2 hours later, and treatment group 2 (T2): paracetamol 1.35g/ kg Body weight and C. zedoaria 105 mg/200g 2 hours later). The Kruskall-Wallis test results showed MDA level did not significantly different between groups (p = 0.087). Hepatocellular changes were observed descriptively with Hematoxylin-eosin staining. Positive control showed greater hepatocellular changes rather than other groups, hepatocyte cells were enlarged with cytoplasm showing eosinophilic granules infiltrates, enlarged irregular nuclei, nucleolus prominent, there are many necrosis cells.

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PENGARUH EKSTRAK DAUN PARE (Momordica charantia) TERHADAP FOLIKEL OVARIUM TIKUS (Rattus norvegicus) DALAM KONDISI HYPERGLYCEMIC

Ovozoa Journal of Animal Reproduction ◽

10.20473/ovz.v8i1.2019.61-65 ◽

2020 ◽

Vol 8 (1) ◽

pp. 61

Author(s):

Ganeswara Muharam Hazmi Rezady ◽

Hani Plumeriastuti ◽

Rimayanti Rimayanti

Keyword(s):

Treatment Group ◽

Alternative Treatment ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Momordica Charantia ◽

Once Daily ◽

Significant Difference

The purpose of this study was to prove that the provision of pare leaf extract can reduce the process of follicular atrophy in the ovaries caused by hyperglycemic conditions. The total of this study was completed within 20 days, including the induction of Streptozotocin for 5 days, treatment for 14 days, and 1 day for ovarian collection. The K- group as a negative control without being induced by Streptozotocin and received no treatment. The K+ group as a positive control was only induced by Streptozotocin at a dose of 55 mg / Kg BW and D10 without treatment. The T1 group was induced by Streptozotocin at doses of 55 mg / Kg BW and D10 and treated with pare leaves extract (Momordica charantia) at a dose of 1.26 ml / Kg BW. T2 group was induced by Streptozotocin with dose of 55 mg / Kg BW and D10 and was treated with pare leaves extract (Momordica charantia) at a dose of 2.52 ml / Kg BW. Induction of Streptozotocin intraperitoneally and once-daily peroral treatment. The result showed that treatment group could make a significant difference with the positive control. Showing that amount of gap could make Momordica charantia to be alternative treatment for hyperglycemic condition.

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Tahu yang Difermentasi Rhizopus sp. Menurunkan Kadar Glukosa Darah Tikus Putih(Rattus norvegicus)

Smart Medical Journal ◽

10.13057/smj.v1i1.24180 ◽

2018 ◽

Vol 1 (1) ◽

pp. 7

Author(s):

Eldaa Putik Bunga Melati ◽

Budiyanti Wiboworini ◽

Briandani Subariyanti

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Rhizopus Sp

Introductions : Controlling of blood glucose would be important to prevent many complications of DM. Consuming fiber is one way to maintain blood glucose level. These amount of fiber could be obtained in tofu fermented by Rhizopus sp. This research aimed to know the effect of tofu fermented by Rhizopus sp. in controlling blood glucose level of diabetic induced white rats (Rattus norvegicus).Methods : This research was a laboratoric-experimental study. The subjects were Rattus norvegicusgrouped randomly. The total 30 Rattus norvegicus were injected by Alloxan 15mg/100gramBW, then randomly allocated into 5 group, positive control group (glibenclamide 0.05mg/100gramBW/day), negative control group (aquadest), and treatment groups with different doses of fermented tofu (0.025gram/100 gramBW/day; 0.050gram/100 gramBW/day; 0.100gram/100gramBW/day). The research design was Pretest and Posttest Control Group Design. The fasting blood glucose were measuredbefore Alloxan injection (day-2), after Alloxan injection (day-5), and after the treatments (day-19).Results : there was a significant decrease of blood glucose level in all treatment groups and positive control (p = 0.001). Post Hoc Test showed a significant difference between all treatment groups and positive control group compared to negative control group (p = 0.001).Conclusion :Tofu fermented by Rhizopus sp. was able to decrease blood glucose level in diabetic rats (Rattus norvegicus). Keyword : Tofu fermented, Rhizopus sp., blood glucose level, alloxan, glibenclamide

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ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSA

JURNAL ILMIAH SAINS ◽

10.35799/jis.13.2.2013.3055 ◽

2013 ◽

Vol 13 (2) ◽

pp. 116 ◽

Cited By ~ 1

Author(s):

Nafila Salma ◽

Jessy Paendong ◽

Lidya I Momuat ◽

Sariyana Togubu

Keyword(s):

Blood Glucose ◽

Treatment Group ◽

Blood Sugar ◽

Plant Extract ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Negative Control ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Male Wistar Rats

ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSAABSTRAK Telah dilakukan penelitian untuk menentukan dosis optimum dari ekstrak tumbuhan suruhan (Peperomia pellucida [L.] Kunth) sebagai antihiperglikemik tikus wistar yang diinduksi sukrosa. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan tikus putih jantan wistar berjumlah 20 ekor tikus yang dibagi dalam 5 kelompok perlakuan yaitu kelompok perlakuan kontrol negatif (K-) diberi CMC 0,5%, kelompok perlakuan kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok perlakuan ekstrak tumbuhan suruhan dosis 20; 40; 80 mg/kgBB. Data diperoleh dari pemeriksaan kadar gula darah puasa (basal), 30 menit setelah diindukasi sukrosa dan pada menit ke 30, 60, dan 120 setelah diinduksi sediaan per oral. Data diolah dengan uji ANOVA dan Ducan untuk membandingkan antara kelompok kontrol negatif, ekstrak dan kontrol positif. Data hasil pengujian kadar glukosa darah dengan dosis 20; 40; 80 mg/kgBB memberikan penurunan kadar glukosa darah. Pemberian ekstrak tumbuhan suruhan dengan dosis 40 mg/kgBB pada menit ke-120 memberikan hasil yang signifikan dan bebeda nyata dibandingkan kelompok kontrol negatif (CMC 0,5%) dan tidak berbeda nyata dengan glibenklamid dosis 0,45 mg/kgBB. Kesimpulannya pemberian ekstrak tumbuhan suruhan dosis 40 mg/kgBB pada menit ke 120 memberikan penurunan kadar glukosa darah yang efektif dan berbeda nyata (signifikan) dibandingkan pemberian ekstrak tumbuhan suruhan dosis 20 dan 80 mg/kgBB. Kata Kunci : Peperomia pellucida [L.] Kunth, Tumbuhan Suruhan, Kadar Glukosa Darah, Sukrosa. ANTIHYPERGLYCEMIC FROM SURUHAN PLANT (Peperomia pellucida [L.] Kunth) EXTRACT AGAINST WISTAR RATS (Rattus norvegicus L.) WITH INCLUDED BY SUCROSE ABSTRACT The purpose of this research is to determine optimum dose of Suruhan (Peperomia pellucida [L.] Kunth) extract as Antihyperglycemic agent against Wistar Rats with Induced by Sucrose. This research using glucose tolerance test methods. Used 20 male Wistar rats were divided into 5 groups, they are the negative control treatment group (K-) were given 0.5% CMC, the positive control groups (K+) given 0,45 mg/kgBB Glibenklamid, and Suruhan extract treatment group of 20; 40; 80 mg/kgBB dose. Data obtained from the examination offasting blood sugar (basal) content, 30 minutes after induced bysucroseandat minute of 30, 60, and120 after-induced oraldosage.The data were processed by ANAVA and LSD testtocompare betweenthe negativecontrol group, the extractandpositive control. Dataresults of testing blood sugar levels with doses of 20, 40, 80 mg/kgBB gavea decreasein blood glucose levels were significant and significantly different than the negative control group (CMC 0.5%) and did not give a clear difference with 0.45 mg/kgBB dose of Glibenclamide. Agents of plant extract at a dose of 40 mg/kgBB body weight gives better results to the decline in blood glucose levels compared with doses of 20 and 80 mg/kgBB. In conclusion, Suruhan plant extract has an effect on blood glucose levels decreased against white male Wistar rats were induced sucrose. Keywords : Peperomia pellucida [L.] Kunth, Suruhan Plant, Blood Glucose Levels, Sucrose

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Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

Folia Medica Indonesiana ◽

10.20473/fmi.v56i4.23411 ◽

2020 ◽

Vol 56 (4) ◽

pp. 269

Author(s):

Devitya Angielevi Sukarno ◽

Arifa Mustika ◽

Purwo Sri Rejeki

Keyword(s):

Insulin Resistance ◽

Blood Glucose ◽

Treatment Group ◽

Rattus Norvegicus ◽

Therapy Group ◽

Fasting Blood Glucose ◽

Apium Graveolens ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apium graveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams, and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay); K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDay and standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days. Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p<0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

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Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

Folia Medica Indonesiana ◽

10.20473/fmi.v56i4.24602 ◽

2021 ◽

Vol 56 (4) ◽

pp. 269

Author(s):

Devitya Angielevi Sukarno ◽

Arifa Mustika ◽

Purwo Sri Rejeki

Keyword(s):

Insulin Resistance ◽

Blood Glucose ◽

Treatment Group ◽

Rattus Norvegicus ◽

Therapy Group ◽

Fasting Blood Glucose ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apiumgraveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams,and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay);K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDayand standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days.Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p <0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

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UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v1i2.103 ◽

2019 ◽

Vol 1 (2) ◽

pp. 11-16

Author(s):

Yanna Rotua Sihombing ◽

Debi Dinha Sitepu

Keyword(s):

Treatment Group ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Delayed Type Hypersensitivity ◽

Hypersensitivity Response ◽

Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno® 32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno® 32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

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Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

Jurnal Katalisator ◽

10.22216/jk.v3i1.2178 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

Verawaty Verawaty ◽

Dhea Claudia Novel

Keyword(s):

Blood Glucose ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

The Third ◽

Blood Glucose Levels

Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.

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UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽

10.35799/pha.10.2021.32767 ◽

2021 ◽

Vol 10 (1) ◽

pp. 736

Author(s):

Grace Laury Tulung ◽

Widdhi Bodhi ◽

Jainer Pasca Siampa

Keyword(s):

Rattus Norvegicus ◽

Design Method ◽

Centella Asiatica ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Flavonoid Compound ◽

Randomized Design ◽

Decrease Blood Glucose ◽

Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords: Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus ABSTRAK Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

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