Paternal melanisation defines wing spot area of male Drosophila nepalensis: supporting evidence through genetic crosses.
Abstract Males of Drosophila nepalensis show dimorphism in wing melanisation but how they evolve and coordinate during evolution is unknown. Heterogeneity in environment helps individuals to adapt accordingly either through genetic polymorphism or through phenotypic plasticity. In this study, we tried to untangle the genetic architecture underlying differences in wing melanisation in males because in nature the frequency of spotted and spotless males is different. We investigated the variation in wing spot formation in males of D. nepalensis via genetic cross. We found wing spot formation on wings in male is directly correlated to female body melanization. We report that the wing spot of D. nepalensis show very high plasticity and correlated with female body melanization instead of male body melanzation. Only at 21°C temperature we found darker and complete light females, dark female progeny always produced male with spotted wing whereas lighter female produced male without wing spot. The degree of wing melanisation in males of D. nepalensis was assessed to check plasticity patterns. We investigated that increased wing spot area (WSA) is negatively correlated with higher temperature. Finally, we find wing spot is highly correlated in reciprocal progeny due to linkage or pleiotropy which could help in evolution.