scholarly journals Association of Cathepsin B a Salivary Biomarkers in Different Histological Grades of Oral Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Alveena Shabbir ◽  
Humera Waheed ◽  
Shaheen Ahmed ◽  
Sabhita Shabir Shaikh
Keyword(s):  
Survival Rate ◽  
Oral Cancer ◽  
Cathepsin B ◽  
Biological Fluids ◽  
Saliva Sample ◽  
Control Group ◽  
Major Public Health Problem ◽  
Salivary Biomarkers ◽  
Poorly Differentiated ◽  
Well Differentiated

Abstract Background:Oral cancer is considered a major public health problem due to its high mortality and morbidity rates. Survival rate of OSCC can be significantly improved by using non -invasive tool such as salivary biomarkers for detection of OSCC which is considered a promising approach. Cathepsin B is a lysosomal cysteine protein, which is present in abundant quantities in lysosome of cells, tissues and different biological fluids. Increased expression of Cathepsin B is observed in many malignancies including oral cancer. The present study was designed to determine the salivary levels of Cathepsin B in different histological grades of OSCC.Method:In this study total no of 80 research participants were enrolled which were divided into four groups. Each group comprises 20 participants. Groups 1 comprises 20 patients of OSCC (Well differentiated), Group 2 comprises 20 patients of OSCC (moderately differentiated), Group 3 comprises 20 patients of OSCC (poorly differentiated|) and Group 4 comprises 20 healthy controls. Saliva sample was collected from all the four study group and salivary Cathepsin B levels were analyzed by ELISA sandwich technique in duplicate.Results:Salivary levels of Cathepsin B are significantly increased with p- value (<0.001) in patients of OSCC in comparison to control group. We observed highest mean Cathepsin B levels in well differentiated OSCC followed by poorly differentiated OSCC and moderately differentiated OSCC.Conclusion:Results of the present study suggests that Cathepsin B has a great value as a salivary biomarker for diagnosis and monitoring of OSCS in different histological grades of OSCC. It could increase the survival rate and further improve the prognosis of OSCC.

scholarly journals Relationship of secretor status in patients with oral cancers

2016 ◽  
Vol 11 (4) ◽  
Author(s):  
Sajid Jamil ◽  
Muhammad Tayyab ◽  
Muhammad Farooq
Keyword(s):  
Oral Cancer ◽  
Saliva Sample ◽  
Control Group ◽  
Healthy Controls ◽  
Oral Cancers ◽  
Secretor Status ◽  
Relationship Of

Present study was designed to find the association of secretor status with oral cancers. In this study 50 diagnosed cases of oral cancers and 50 healthy controls were selected. Secretor status on saliva sample was performed in all these subjects. 39 (78%) subjects were secretors and 11 (22%) were non secretors in control group. 28 (56%) patients with oral cancers were secretors and 22 (44%) were non secretors. Conclusions: Oral cancer was found to be associated with non-secretor status.

scholarly journals Survival Rate of Gastric Cancer Patients in Jordan: Secondary Data Analysis (Preprint)

2019 ◽  
Author(s):  
Ashraf Aqel ◽  
Yousef Khader ◽  
Kamal Arqoub ◽  
Omar Nimri
Keyword(s):  
Gastric Cancer ◽  
Survival Rate ◽  
Eastern Mediterranean ◽  
Secondary Analysis ◽  
Eastern Mediterranean Region ◽  
Secondary Data ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Gastric Cancer Patients

BACKGROUND Gastric cancer accounts for 2.7% of all newly diagnosed cancer cases in Jordan. OBJECTIVE The aim of this study was to calculate the survival rate and its determinants among Jordanian patients who were diagnosed with gastric cancer between 2010 and 2014. METHODS A descriptive study was conducted based on secondary analysis of data from the Jordan Cancer Registry during the period of 2010-2014. Only cancer-related deaths were recorded as “death” in the survival analysis. RESULTS A total of 1388 new cases of gastric cancer were recorded between 2010 and 2014. Of these, 872 (62.8%) were Jordanians and 60.5% were males. The mean age at diagnosis was 58.9 years and the median follow-up time was 1.6 years. The 5-year survival rate decreased significantly from 89% in patients with well-differentiated cancer to 32% in patients with poorly differentiated cancer (<i>P</i>=.005). The overall 5-year survival rate was 37.7% and the median survival was 1.48 years (95% CI 1.179-1.783). The 5-year survival rate decreased significantly with increasing age and with advanced stage of the disease: the 5-year survival rate was 75% for localized-stage, 48% for regional-stage, and 22.7% for distant-metastasis disease (<i>P</i>=.005). CONCLUSIONS This study showed that the overall 5-year survival rate among patients with gastric cancer in Jordan between 2010 and 2014 was 37.7%, which is higher than the reported rates from different countries in the Eastern Mediterranean region such as Egypt.

scholarly journals Survival Rate of Gastric Cancer Patients in Jordan: Secondary Data Analysis

10.2196/14359 ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. e14359
Author(s):  
Ashraf Aqel ◽  
Yousef Khader ◽  
Kamal Arqoub ◽  
Omar Nimri
Keyword(s):  
Gastric Cancer ◽  
Survival Rate ◽  
Eastern Mediterranean ◽  
Secondary Analysis ◽  
Eastern Mediterranean Region ◽  
Secondary Data ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Gastric Cancer Patients

Background Gastric cancer accounts for 2.7% of all newly diagnosed cancer cases in Jordan. Objective The aim of this study was to calculate the survival rate and its determinants among Jordanian patients who were diagnosed with gastric cancer between 2010 and 2014. Methods A descriptive study was conducted based on secondary analysis of data from the Jordan Cancer Registry during the period of 2010-2014. Only cancer-related deaths were recorded as “death” in the survival analysis. Results A total of 1388 new cases of gastric cancer were recorded between 2010 and 2014. Of these, 872 (62.8%) were Jordanians and 60.5% were males. The mean age at diagnosis was 58.9 years and the median follow-up time was 1.6 years. The 5-year survival rate decreased significantly from 89% in patients with well-differentiated cancer to 32% in patients with poorly differentiated cancer (P=.005). The overall 5-year survival rate was 37.7% and the median survival was 1.48 years (95% CI 1.179-1.783). The 5-year survival rate decreased significantly with increasing age and with advanced stage of the disease: the 5-year survival rate was 75% for localized-stage, 48% for regional-stage, and 22.7% for distant-metastasis disease (P=.005). Conclusions This study showed that the overall 5-year survival rate among patients with gastric cancer in Jordan between 2010 and 2014 was 37.7%, which is higher than the reported rates from different countries in the Eastern Mediterranean region such as Egypt.

scholarly journals Ovarian cancer in Alexandria from 1988 to 1997: trends and survival

1999 ◽  
Vol 5 (4) ◽  
pp. 727-739
Author(s):  
N. H. Mahdy ◽  
M. Abdel Fattah ◽  
H. Ghanem
Keyword(s):  
Ovarian Cancer ◽  
Prognostic Factors ◽  
Survival Rate ◽  
Cancer Registry ◽  
Medical Records ◽  
Survival Rates ◽  
Significant Survival ◽  
Increasing Trend ◽  
Poorly Differentiated ◽  
Well Differentiated

The trend of incidence of ovarian cancer was studied, the 5-year survival rate calculated and prognostic factors for survival determined. Data were collected from the Alexandria Cancer Registry and medical records in various hospitals. A total of 358 cases of ovarian cancer were diagnosed from 1988 to 1997. A significant increasing trend in incidence of ovarian cancer from 1.23/100 000 in 1988 to 3.16/100 000 in 1997 was found. The overall 5-year survival rate was 46%. The 5-year survival rates for tumour stages I to IV were 85%, 71%, 41% and 22% respectively, which was statistically significant. Survival rates with poorly differentiated tumours were significantly worse than with moderate or well differentiated tumours

scholarly journals A prospective study for Assessment of saliva CA- 125 level in oral cancer patients

2021 ◽  
Vol 15 (7) ◽  
pp. 1922-1925
Author(s):  
Arafat Ahmad ◽  
Usama Bin Ghaffar ◽  
Sanket Dadarao Hiware ◽  
Mohammed Taher Ali ◽  
Samina Wasi
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Stage Iv ◽  
Ca 125 ◽  
Group I ◽  
Whole Saliva ◽  
Oral Cancer Patients ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
A Prospective Study

Aim: To detect CA- 125 level in saliva of oral cancer patients. Methodology: A total of fifty- six SCC patients and thirty healthy subjects were selected. A non- stimulated whole saliva (5cc) was collected and evaluated for CA- 125 level ELISA. Results: A higher salivary CA 125 level (514.2±132.6 U/mL) was observed in poorly differentiated SCC followed by moderately differentiated (340.6±80.2 U/mL) and minimum values was observed in well differentiated SCC (236.2±76.2 U/mL). The mean± SD CA- 125 level in group I patients was 428.5± 110.2 U/mL and in group II was 132.4± 58.6 U/mL. Higher salivary CA 125 level (520.5±168.4 U/mL) was observed in stage IV and minimum in stage I (165.2±46.2 U/mL) which was statistically significant (P< 0.05). Conclusion: Patients with poorly differentiated SCC, cases of buccal mucosa and stage IV exhibited higher values of salivary CA- 125 level as compared to healthy control. Key words: Oral Cancer, Saliva, Tongue, CA- 125

scholarly journals Cyclin D1 expression of different histological grades in oral squamous cell carcinoma patients from northern India

2018 ◽  
Vol 24 (1) ◽  
pp. 6-9
Author(s):  
Manzoor Gatoo ◽  
Ayaz Dar
Keyword(s):  
Oral Cancer ◽  
Cyclin D1 ◽  
Primary Site ◽  
Floor Of Mouth ◽  
Northern India ◽  
Significant Difference ◽  
Poorly Differentiated ◽  
Well Differentiated ◽  
Degree Of Differentiation ◽  
Differentiation Of Tumors

Cyclin D1 expression was positive in 35 cases of oral cancer with a Cyclin D1 positivity of 22.16?22.18. The percentage of positive cases as well as Cyclin D1 positivity showed an increase as the grade of differentiation advanced. No significant association was found between Cyclin D1 positivity and degree of differentiation of tumors (p=0.138). A significant difference in Cyclin D1 positivity was observed (p=0.043) comparing well differentiated (16.61?17.89) and poorly differentiated (37.0?32.51) tumors, as well as between well differentiated (16.61?17.89) and moderately differentiate tumors (24.38?21.93; p=0.002). Similarly, significant difference in Cyclin D1 positivity was observed comparing moderately differentiated (24.38?21.93) and poorly differentiated tumors (37.0?32.51; p=0.043). Cyclin D1 expression was more frequently seen in hard palate (75%), buccal mucosa (67%) and lip (60%) while expression of Cyclin D1 was less frequent in sites like gingiva (0%), tongue (40%) and floor of mouth (43%). There was no association between Cyclin D1 expression and primary site of oral cancer (p=0.528) in tobacco and betel quid chewers of northern India.

Motility of leukemic lymphocytes

1990 ◽  
Vol 48 (3) ◽  
pp. 368-369
Author(s):  
Manoj Raje ◽  
Karvita B. Ahluwalia
Keyword(s):  
Quantitative Data ◽  
Laser Doppler Velocimetry ◽  
Acute Lymphocytic Leukemia ◽  
Lymphocytic Leukemia ◽  
Cellular Space ◽  
Differentiated Cells ◽  
Poorly Differentiated ◽  
Solid Tissues ◽  
Well Differentiated ◽  
Reduced Mobility

In Acute Lymphocytic Leukemia motility of lymphocytes is associated with dissemination of malignancy and establishment of metastatic foci. Normal and leukemic lymphocytes in circulation reach solid tissues where due to in adequate perfusion some cells get trapped among tissue spaces. Although normal lymphocytes reenter into circulation leukemic lymphocytes are thought to remain entrapped owing to reduced mobility and form secondary metastasis. Cell surface, transmembrane interactions, cytoskeleton and level of cell differentiation are implicated in lymphocyte mobility. An attempt has been made to correlate ultrastructural information with quantitative data obtained by Laser Doppler Velocimetry (LDV). TEM of normal & leukemic lymphocytes revealed heterogeneity in cell populations ranging from well differentiated (Fig. 1) to poorly differentiated cells (Fig. 2). Unlike other cells, surface extensions in differentiated lymphocytes appear to originate by extrusion of large vesicles in to extra cellular space (Fig. 3). This results in persistent unevenness on lymphocyte surface which occurs due to a phenomenon different from that producing surface extensions in other cells.

HER2 OVEREXPRESSION IN ENDOSCOPIC BIOPSY SAMPLE OF GASTRIC ADENOCARCINOMA BY IMMUNOHISTOCHEMISTRY

2012 ◽  
pp. 109-118
Author(s):  
Viet Nho Le ◽  
Van Huy Tran ◽  
Cong Thuan Dang ◽  
Van To Ta
Keyword(s):  
Gastric Adenocarcinoma ◽  
Undifferentiated Carcinoma ◽  
Signet Ring Cell Carcinoma ◽  
Signet Ring Cell ◽  
Endoscopic Biopsy ◽  
Her2 Overexpression ◽  
Tubular Adenocarcinoma ◽  
Signet Ring ◽  
Poorly Differentiated ◽  
Well Differentiated

Background and aim: HER2 overexpression by immunohistochemistry is a prognostic maker in gastric cancer and helps to select candidates benefitted from targeted therapy with trastuzumab. This study is aimed at the assessing HER2 overexpression and its relationship with endoscopic and histopathological findings of gastric adenocarcinoma. Objectives and methods: Biopsy samples from 92 gastric cancer patients were examined for HER2 status by immunohistochemical staining. Results: 6.5% of tumors were cardia tumors and 93.5% were non-cardia tumors. Using the Lauren classification, 51.1% were intestinal type and 48.9% were diffuse type. Using WHO classification, 54.3% were tubular adenocarcinoma, 7.6% were mucinous adenocarcinoma, 15.2% were signet-ring cell carcinoma, and 22.8% were undifferentiated carcinoma. 32.6% were well-differentiated, 15.2% were moderately-differentiated, and 52.2% were poorly-differentiated carcinoma. HER2 was positive in 20.7% of gastric carcinomas, 50% cardia tumors and 18.6% non-cardia tumors. HER2 positivity among polypoid, fungating, ulcerated, and infiltrative types were 38.5%, 29.7%, 9.1% and 0%, respectively. HER2 overexpression in intestinal type was higher than that in diffuse type (31.9% vs. 8.9%, p = 0.009). HER2 overexpression in tubular adenocarcinoma, mucinous adenocarcinoma, signet-ring cell carcinoma, and undifferentiated carcinoma was 28.0%, 14.3%, 7.1% and 14.3%, respectively. HER2 overexpressions were different between differentiation degrees: 30% of well-differentiated tumors, 35.7% moderately-differentiated tumors, and 10.4% of poorly-differentiated tumors (p = 0.037). Conclusions: HER2 overexpression was found in 20.7% of endoscopic biopsy sample of gastric adenocarcinoma and was associated with endoscopic gross characteristic, Lauren histologic type and differentiation degree.

Salivary mir-27b Expression in Oral Lichen Planus Patients: A Series of Cases and a Narrative Review of Literature

2020 ◽  
Vol 19 (31) ◽  
pp. 2816-2823 ◽  
Author(s):  
Dario Di Stasio ◽  
Laura Mosca ◽  
Alberta Lucchese ◽  
Donatella Delle Cave ◽  
Hiromichi Kawasaki ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Inflammatory Diseases ◽  
Biological Fluids ◽  
Critical Role ◽  
Invasive Procedure ◽  
Control Group ◽  
Study Group ◽  
Immune Functions ◽  
Oral Lichen

Background: microRNAs play a critical role in auto-immunity, cell proliferation, differentiation and cell death. miRNAs are present in all biological fluids, and their expression is essential in maintaining regular immune functions and preventing autoimmunity, whereas miRNA dysregulation may be associated with the pathogenesis of autoimmune and inflammatory diseases. Oral lichen planus (OLP) is an inflammatory disease mediated by cytotoxic T cells attack against epithelial cells. The present study aims to perform a specific microRNA expression profile through the analysis of saliva in this disease. Methods: The study group was formed by five patients (mean age 62.8±1.98 years; 3 females/2 males) affected by oral lichen planus and control group by five healthy subjects (mean age 59.8 years±2.3; 3 females/ 2 males); using a low-density microarray analysis, we recorded a total of 98 differentially expressed miRNAs in the saliva of patients with oral lichen planus compared to the control group. The validation was performed for miR-27b with qRT-PCR in all saliva samples of oral lichen planus group. Results: 89 miRNAs were up-regulated and nine down-regulated. In details, levels of miR-21, miR- 125b, miR-203 and miR15b were increased (p<0.001) in study group while levels of miR-27b were about 3.0-fold decreased compared to controls (p<0.001) of miR-27b expression in OLP saliva. QRTPCR validation confirmed the down regulation of miR-27b in all saliva samples. Conclusions: Collecting saliva samples is a non-invasive procedure and is well accepted by all patients. microRNAs can be readily isolated and identified and can represent useful biomarkers of OLP.

Effectiveness of mobile applications on healthy lifestyles aimed at diabetic patients: review of systematic reviews. (Preprint)

2019 ◽  
Author(s):  
Francisco Jesús Represas Carrera Sr ◽  
Ángel Alfredo Martínez Ques Sr ◽  
Ana Clavería Fontán Sr
Keyword(s):  
Quality Of Life ◽  
Diabetes Mellitus ◽  
Systematic Reviews ◽  
Mobile Applications ◽  
Control Group ◽  
Diabetic Patients ◽  
Healthy Lifestyles ◽  
Major Public Health Problem

BACKGROUND Diabetes mellitus is currently a major public health problem worldwide. It is traditionally approached in a clinical inpatient relationship between the patient and the healthcare professional. However, the rise of new technologies, particularly mobile applications, is revolutionizing the traditional healthcare model through the introduction of telehealthcare. OBJECTIVE (1) To assess the effects of mobile applications for improving healthy lifestyles on the quality of life and metabolic control of diabetes mellitus in adult patients. (2) To describe the characteristics of the mobile applications used, identify the healthy lifestyles they target, and describe any adverse effects their use may have. METHODS Review of systematic reviews and meta-analysis, following the guidelines of the Cochrane Collaboration and the Joanna Briggs Institute. We included studies that used any mobile application aimed at helping patients improve self-management of diabetes mellitus by focusing on healthy lifestyles. Studies needed to include a control group receiving regular care without the use of mobile devices. In May 2018, a search was conducted in Medline, Embase, Cochrane, LILACS, PsychINFO, Cinahl and Science Direct, updated in May 2019. The methodological quality of the studies was assessed using the Amstar-2 tool. RESULTS Seven systematic reviews of 798 articles were initially selected for analysis. The interventions had a duration of between 1 and 12 months. Mobile applications focused singly or simultaneously on different lifestyles aspects (diet, physical exercise, motivation, blood glucose levels, etc.). There are significant changes in HbA1c values, body weight and BMI, although in others, such as lipid profile, quality of life, or blood pressure, there is no clear improvement. CONCLUSIONS There is clear evidence that the use of mobile applications improves glycemic control in diabetic patients in the short term. There is a lack of evidence in its long-term benefits. It is thus necessary to carry out further studies to learn about the long-term effectiveness of mobile applications aimed at promoting the healthy lifestyles of diabetic patients. CLINICALTRIAL PROSPERO Register: CRD42019133685

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