scholarly journals Quorum Sensing Mediated Pathogenicity, Virulence Genes and Class 1 Integron in Carbapenem-Resistant Clinical Pseudomonas Aeruginosa Isolates

2021 ◽  
Author(s):  
Ceren Başkan ◽  
Belgin Sırıken ◽  
Enis Fuat Tüfekci ◽  
Çetin Kılınç ◽  
Ömer Ertürk ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Virulence Genes ◽  
Culture Method ◽  
Isolation And Identification ◽  
Class 1 Integron ◽  
Positive Correlation ◽  
Conventional Culture ◽  
Carbapenem Resistant ◽  
Slime Production ◽  
Class 1

Abstract Carbapenems are the most effective agents for treating carbapenem-resistant clinical P. aeruginosa (CRPsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence genes and a class 1 (Cls1) integron. A total of 52 CRPsA isolates obtained from Kastamonu, Turkey was analyzed. A conventional culture method, an oprL gene-based molecular assay for P. aeruginosa isolates, and an automated VITEK-2 compact system were applied for the isolation and identification of CRPsA isolates. The oprL gene was detected in all of the isolates tested. At least one of the las or rhl system genes was detected in 98.07% of the isolates, and the percentage of las system genes (98.07%) were higher than that of rhl system genes (90.38%). algD, lasB, toxA and aprA genes were detected in between 46.15% and 88.46% of the isolates, and co-existence of four genes were detected in 40.38% of the isolates. Cls1 integron and slime production using Congo Red Agar (CRA) were present in 51.92% and 67.30%, respectively, of the isolates. There was a significant positive correlation (p <.10) between the las system and the rhl system and a strongly positive correlation (p<.01 or p <.05) between the rhl system-four virulence genes and slime production-and among some virulence genes. In conclusion, the CRPsA isolates tested in the study are highly virulent and QS systems have a significant role in pathogenesis.

scholarly journals Molecular characterisation of antimicrobial resistance and virulence genes in Escherichia coli strains isolated from diarrhoeic and healthy rabbits in Tunisia

2020 ◽  
Vol 28 (2) ◽  
pp. 81
Author(s):  
Raouia Ben Rhouma ◽  
Ahlem Jouini ◽  
Amira Klibi ◽  
Safa Hamrouni ◽  
Aziza Boubaker ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Antibiotic Resistance Genes ◽  
Virulence Gene ◽  
Diffusion Method ◽  
Class 1 Integron ◽  
E Coli ◽  
Class 1

The purpose of this study was to identify <em>Escherichia coli</em> isolates in diarrhoeic and healthy rabbits in Tunisia and characterise their virulence and antibiotic resistance genes. In the 2014-2015 period, 60 faecal samples from diarrhoeic and healthy rabbits were collected from different breeding farms in Tunisia. Susceptibility to 14 antimicrobial agents was tested by disc diffusion method and the mechanisms of gene resistance were evaluated using polymerase chain reaction and sequencing methods. Forty <em>E. coli</em> isolates were recovered in selective media. High frequency of resistance to tetracycline (95%) was detected, followed by different levels of resistance to sulphonamide (72.5%), streptomycin (62.5%), trimethoprim-sulfamethoxazole (60%), nalidixic acid (32.5%), ampicillin (37.5%) and ticarcillin (35%). <em>E. coli</em> strains were susceptible to cefotaxime, ceftazidime and imipenem. Different variants of bla<sub>TEM</sub>, <em>tet</em>, <em>sul</em> genes were detected in most of the strains resistant to ampicillin, tetracycline and sulphonamide, respectively. The presence of class 1 integron was studied in 29 sulphonamide-resistant <em>E. coli</em> strains from which 15 harboured class 1 integron with four different arrangements of gene cassettes, <em>dfrA17</em>+<em>aadA5</em> (n=9), <em>dfrA1</em> + <em>aadA1</em> (n=4), <em>dfrA12</em> + <em>addA2</em> (n=1), <em>dfrA12</em>+<em>orf</em>+<em>addA2</em> (n=1). The <em>qnrB</em> gene was detected in six strains out of 13 quinolone-resistant <em>E. coli</em> strains. Seventeen <em>E. coli</em> isolates from diarrhoeic rabbits harboured the enteropathogenic eae genes associated with different virulence genes tested (<em>fimA</em>, <em>cnf1</em>, <em>aer</em>), and affiliated to B2 (n=8) and D (n=9) phylogroups. Isolated <em>E. coli</em> strains from healthy rabbit were harbouring <em>fim A</em> and/or <em>cnf1</em> genes and affiliated to A and B1 phylogroups. This study showed that <em>E. coli</em> strains from the intestinal tract of rabbits are resistant to the widely prescribed antibiotics in medicine. Therefore, they constitute a reservoir of antimicrobial-resistant genes, which may play a significant role in the spread of antimicrobial resistance. In addition, the eae virulence gene seemed to be implicated in diarrhoea in breeder rabbits in Tunisia.

scholarly journals A Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Gabriele Arcari ◽  
Federica Maria Di Lella ◽  
Giulia Bibbolino ◽  
Fabio Mengoni ◽  
Marzia Beccaccioli ◽  
...  
Keyword(s):  
Host Range ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Gene Cassette ◽  
University Hospital ◽  
Broad Host Range ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACT In this study, we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, blaVIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the blaSHV-12, qnrS1, and mph(A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.

scholarly journals Emergence of OXA-10 and OXA-48 like carbapenemases among Enterobacter isolates from inpatients in southern Iran

2021 ◽  
Author(s):  
Melika Moradi ◽  
Reza Khashei ◽  
Yalda Malekzadegan ◽  
Jamal Sarvari
Keyword(s):  
Carbapenem Resistance ◽  
Common Species ◽  
Resistance Rate ◽  
Health Concern ◽  
Multi Drug Resistance ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
Microbiological Methods ◽  
Global Spread ◽  
Class 1

Abstract Background The global spread of carbapenemase-producing Enterobacteriaceae represents a public health concern. The aim of this study was to investigate prevalence of carbapenem resistance, oxacillinase types and the presence of class 1–3 integrons among Enterobacter clinical isolates from an Iranian inpatients’ population. Methods Ninety Enterobacter isolates recovered from hospitalized patients were diagnosed by the standard microbiological methods. Antibiogram pattern was also determined. The presence of class 1–3 integrons and four types of oxacillinase genes were assessed using PCR. Results Of the 90 Enterobacter isolates, the most common species was identified as E. aerogenes, (45.6%), followed by E. cloacae (30%). The highest resistance rate was against to ampicillin (96.7%). Multi-drug resistance (MDR) was substantial (93%). Carbapenemase producers were detected in 96% of carbapenem resistant isolates by mCIM test. The frequency of evaluated genes was as follows: intI1 = 50 (55.6%), intI2 = 12 (13.3%), blaoxa−1 =6 (6.7%), blaoxa−2 =5 (5.6%), blaoxa−10 =18 (20%), and blaoxa−48 =18 (20%). Conclusion The determinants of class 1 integron with OXA-10 and OXA-48 like carbapememases have been responsible of relatively considerable of carbapenem resistance among isolates. This is the first OXA-10 and OXA-48-producing Enterobacter spp. in Iran, indicating that the prevalence of oxacillinases might be on the rise in country.

scholarly journals First Occurrence of the OXA-198 Carbapenemase in Enterobacterales

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Rémy A. Bonnin ◽  
Agnès B. Jousset ◽  
Lauraine Gauthier ◽  
Cécile Emeraud ◽  
Delphine Girlich ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Routine Screening ◽  
Conjugative Plasmid ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
Class 1 ◽  
First Occurrence

ABSTRACT A carbapenem-resistant Citrobacter sp. was recovered from routine screening of multidrug-resistant bacteria. This isolate coproduced OXA-48 and OXA-198. OXA-48 was carried by the prototypical IncL plasmid, whereas OXA-198 was carried by a peculiar IncHI-type plasmid. This carbapenemase gene was inserted within a class 1 integron located on a conjugative plasmid. This report describes the first occurrence of OXA-198 in Enterobacterales.

scholarly journals Molecular Analyses of Biofilm-Producing Clinical Acinetobacter baumannii Isolates from a South Indian Tertiary Care Hospital

2020 ◽  
Vol 29 (6) ◽  
pp. 580-587
Author(s):  
Balaram Khamari ◽  
Manmath Lama ◽  
Chanakya Pachi Pulusu ◽  
Amarendra Pratap  Biswal ◽  
Sai Manoz Lingamallu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Virulence Genes ◽  
Random Amplified Polymorphic Dna ◽  
Tertiary Care ◽  
Care Hospital ◽  
Drug Resistant ◽  
Class 1 Integron ◽  
Biofilm Production ◽  
Class 1

<b><i>Objectives:</i></b> The aim of the study was to determine the presence of antimicrobial-resistance (AMR) genes, virulence genes, and mobile genetic elements (MGEs) in 14 biofilm-producing clinical isolates of <i>Acinetobacter baumannii</i>. <b><i>Materials and Methods:</i></b> PCR amplification was performed to analyse the prevalence of genes associated with antibiotic resistance (extended-spectrum β-lactamases [ESBLs] and metallo-β-lactamases [MBLs]), virulence factors, MGEs (class 1 integron, Tn<i>1213</i>, and <i>A. baumannii</i> antibiotic resistance [AbaR]), and <i>comM</i> among the study isolates. Random amplified polymorphic DNA (RAPD) PCR was then deployed to understand their phylogenetic relationship. All the isolates were investigated for biofilm production. <b><i>Results:</i></b> Two isolates were antibiotic-sensitive (AS), 3 were multi-drug-resistant (MDR), and the remaining 9 were extensively drug-resistant (XDR). The majority of the isolates were found to be positive for biofilm production and were sensitive against tetracycline and colistin only. Ab14 and Ab11 were found to be resistant to minocycline and colistin, respectively. <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>OXA</sub>, <i>bla</i><sub>NDM</sub>, <i>bla</i><sub>VIM</sub>, <i>bla</i><sub>SIM</sub>, and <i>bla</i><sub>PER-1</sub>; class 1 integron; composite transposon Tn<i>1213</i>; AbaR island, and virulence factor genes were detected among the isolates. These pathogens were found to have originated from multiple clonal lineages. <b><i>Conclusion:</i></b> Biofilm-producing <i>A. baumannii</i> with multiple virulence and AMR genes pose serious clinical challenges. The presence of MGEs further compounds the situation as these isolates serve as potential reservoirs of AMR and virulence genes. Together with their capacity for natural competence, <i>A. baumannii</i>, if left unchecked, will lead to the spread of resistance determinants to previously sensitive bacteria and may aid in the emergence of untreatable pan-drug-resistant phenotypes.

scholarly journals Metallo-β-Lactamase Gene blaIMP-15 in a Class 1 Integron, In95, from Pseudomonas aeruginosa Clinical Isolates from a Hospital in Mexico

2008 ◽  
Vol 52 (8) ◽  
pp. 2943-2946 ◽  
Author(s):  
U. Garza-Ramos ◽  
R. Morfin-Otero ◽  
H. S. Sader ◽  
R. N. Jones ◽  
E. Hernández ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Gene Cassette ◽  
Clinical Isolates ◽  
Care Hospital ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Lactamase Gene

ABSTRACT During 2003, 40 carbapenem-resistant Pseudomonas aeruginosa clinical isolates collected in a Mexican tertiary-care hospital were screened for metallo-β-lactamase production. Thirteen isolates produced IMP-15, and 12 had a single pulsed-field gel electrophoresis pattern. The bla IMP-15 gene cassette was inserted in a plasmid-borne integron with a unique array of gene cassettes and was named In95.

scholarly journals Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

2012 ◽  
Vol 56 (5) ◽  
pp. 2746-2749 ◽  
Author(s):  
Sanda Sardelic ◽  
Branka Bedenic ◽  
Céline Colinon-Dupuich ◽  
Stjepan Orhanovic ◽  
Zrinka Bosnjak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Coastal Region ◽  
Large Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Variable Part ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Sequence Types

ABSTRACTOne hundred sixty-nine nonreplicate imipenem-resistantPseudomonas aeruginosastrains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried theblaVIM-2gene located in a novel class 1 integron composed in its variable part of theblaVIM-2-blaoxa-10-ΔqacF-aacA4genes. Metallo-β-lactamase-producing strains belonged to sequence types ST235 and ST111.

scholarly journals Isolation of an Integron-Borne blaVIM-4 Type Metallo-β-Lactamase Gene from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate in Hungary

2004 ◽  
Vol 48 (9) ◽  
pp. 3576-3578 ◽  
Author(s):  
Balázs Libisch ◽  
Mária Gacs ◽  
Károly Csiszár ◽  
Mónika Muzslay ◽  
László Rókusz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Structural Similarity ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
High Structural Similarity ◽  
Class 1 ◽  
Lactamase Gene

ABSTRACT The first integron-borne metallo-β-lactamase gene was isolated in Hungary. The bla VIM-4 gene is located on a class 1 integron that also carries a novel bla OXA-like gene. The integron is harbored by a serotype O12 Pseudomonas aeruginosa strain and shows high structural similarity to integrons isolated in Greece and Poland.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

Sign in / Sign up

Export Citation Format

Share Document