The role of ICOSL in children with neutrophilic asthma

2020 ◽  
Author(s):  
Heting Dong ◽  
Wujun JIANG ◽  
Li HUANG ◽  
Meijuan WANG ◽  
Yongdong YAN ◽  
...  
Keyword(s):  
Diagnostic Criteria ◽  
Lavage Fluid ◽  
Eosinophil Infiltration ◽  
Control Group ◽  
Hospitalization Time ◽  
Children With Asthma ◽  
Molecular Ligand ◽  
Ligand Expression ◽  
Group A ◽  
Asthma Group

Abstract Background: It has been shown that certain severe and refractory asthma cases are due to neutrophil and not eosinophil infiltration. ICOSL (Inducible costimulatory molecular ligand) expression is closely associated with tumor and autoimmune diseases, while a limited amount of data has been published regarding the significance of ICOSL in children with neutrophilic asthma. The present study aimed to explore the abnormal expression of ICOSL in peripheral blood and bronchoalveolar lavage fluid (BALF) samples of children with neutrophilic asthma and their clinical significance. Methods: The present clinical study selected children who met the diagnostic criteria of asthma from the children's Hospital of Suchow University and excluded the patients with positive etiology. The children who were admitted to the hospital for foreign body inhalation in the same period were collected as the control group. The children with more than 50% (the percentage of neutrophils in BALF was 95% of the percentile in the control group) of neutrophils in BALF samples were assigned to the neutrophilic asthma group (NA group), whereas the remaining subjects comprised the asthma group (A group). The expression levels of ICOSL, IL-4, IL-17, IFN-, neutrophil elastase (NE) and matrix metalloproteinase (MMP)-9 were detected in plasma and BALF samples by enzyme-linked immunosorbent assays in order to analyze the differences in the levels of cytokines and clinical characteristics between children with neutrophilic asthma and non-neutrophilic asthma. Moreover, the potential mechanism of ICOSL in neutrophilic asthma was explored. Results: In strict accordance with the diagnostic criteria of asthma and following exclusion of pathogenic positive children, 32 children were finally enrolled, including 12 children in the NA group and 20 children in the A group. The mean hospitalization time of the NA group was longer than that of the A group (P<0.05). The concentration levels of ICOSL, IL-17, NE and MMP9 of the NA group in plasma and BALF samples were higher than those in the A group, while the levels of IFN-γ exhibited the opposite trend. A significant correlation was evident between ICOSL and IL-17 levels in plasma（r=0.753，P=0.012） and BALF（r=0.774，P=0.009）samples of the NA group. Conclusion: Children with asthma exhibited an immunity imbalance of Th1/Th2/Th17, whereas neutrophilic asthma children were more severely affected. The clinical treatment was considerably difficult and the hospitalization time was longer. ICOSL may regulate the secretion of IL-17 by Th17 and increase the levels of NE and MMP9, which are involved in the development of immune inflammation in neutrophils.

MicroRNA in Children with Asthma: Findings from Bronchoalveolar Lavage Fluid

2021 ◽  
Author(s):  
Min Liu ◽  
Jian-Hai Lv ◽  
Lei Wu ◽  
Lan-Fang Tang
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Target Genes ◽  
Lavage Fluid ◽  
Control Group ◽  
Qrt Pcr ◽  
Differentially Expressed Mirnas ◽  
Children With Asthma ◽  
Asthma Group ◽  
E Cadherin

Abstract Objective: To investigate the pathogenesis of miRNA in asthma by measuring the microRNAs (miRNAs) levels in the bronchoalveolar lavage fluid (BALF) cells of children with asthma.Methods: A total of 15 children with asthma were enrolled as the asthma group and another 13 children who were subjected to airway foreign removal within 24 hours were enrolled as the control group. The levels of miRNAs were measured by miRNA microarray and verified by qRT-PCR. The expression of target genes E-cadherin was assessed as well.Results: A total of 65 miRNAs in the BALF cells of the asthma group were different from those in the control group. Six miRNAs of miR-34/449 family and seven miRNAs of miR-200 family were decreased. For 13 differentially expressed miRNAs, qRT-PCR confirmed that 10 miRNAs were consistent with the results of miRNAs microarray, including miR-34b-3p, miR-34b-5p, miR-34c-3p, miR-34c-5p, miR-200a-3p, miR-200a-5p, miR-200b-3p, miR-200b-5p, miR-200c-3p, miR-141-3p. E-cadherin, the predicted target of miR-34/449 family and miR-200 family, was down-regulated in children with asthma as well.Conclusions: These data suggested that miRNAs, especially the miR-34/449 and miR-200 families, were involved in the mechanism of childhood asthma by regulating E-cadherin expression. Further studies on the mechanism of miRNAs in asthma are required.

scholarly journals Behavioural Problems and Parental Over-protectiveness in Children with Asthma

2017 ◽  
Vol 36 (3) ◽  
pp. 232-237
Author(s):  
Neena Sanjiv Sawant ◽  
Juhi Bhargava ◽  
Chandrahas T. Deshmukh
Keyword(s):  
Demographic Variables ◽  
Behavioural Problems ◽  
Control Group ◽  
Behaviour Problem ◽  
Early Referral ◽  
Parental Awareness ◽  
Children With Asthma ◽  
Minor Ailments ◽  
And Control ◽  
Asthma Group

Introduction: Asthma in children often leads to behavioural problems due to the chronic nature of the disease. Parents also tend to be overprotective and many may not even consult a doctor for the same. The objectives were to study the various behavioural problems seen in children having asthma as compared to those with minor ailments, parental awareness about the same and their parenting styles.Material and Methods: 75 parents of children of bronchial asthma and 75 parents of children having short term minor ailments formed the asthma and control groups respectively. The Behaviour Problem Index was used to assess the behavioural problems in children as documented by parents and parental overprotectivenesswas assessed using the Vulnerable Child/ Overprotecting Parent Scale.Results:The demographic variables were comparable in both the groups. Behavioural problems on all domains and parental over protectiveness in the asthma group were found to be statistically significant as compared to control group. However there was no significant correlation between the parental over-protectivenessand the behavioural problems in both the groups.Conclusions: Early referral to the psychiatrist with parental counselling will result in better outcomes for the child.J Nepal Paediatr Soc 2016;36(3):232-237

scholarly journals Evaluation of oral functions of the stomatognathic system according to the levels of asthma severity

2012 ◽  
Vol 24 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Mariana San Jorge de Castro ◽  
Adyléia Aparecida Dalbo Contrera Toro ◽  
Eulália Sakano ◽  
José Dirceu Ribeiro
Keyword(s):  
Persistent Asthma ◽  
Asthma Severity ◽  
Control Group ◽  
Healthy Children ◽  
Structural Part ◽  
Assessment Protocol ◽  
Group I ◽  
Study Results ◽  
Children With Asthma ◽  
Asthma Group

PURPOSE: To compare the orofacial functions (chewing, swallowing and speech) in children with asthma and healthy children. METHODS: A cross sectional study including 54 children of both genders with ages between 7 and 10 years was conducted. Twenty-seven of these subjects composed the experimental group, and were subdivided into two severity levels of asthma: Group I - mild intermittent and persistent asthma; Group II - persistent moderate to severe asthma. Twenty-seven healthy children were included in the control group (Group III). Speech-language pathology evaluation used the adapted Orofacial Myofunctional Assessment Protocol. Adaptation consisted in the exclusion of the structural part of the test, since this was not the aim of the study. The structural part was excluded because it was not the aim of this study. RESULTS: It was found alterations in oral functions, with significant differences between the three groups. These alterations showed no correlation with asthma severity, since the highest rate of alterations was found in Group I (mild asthma). CONCLUSION: Regardless of the severity level, children with asthma have altered patterns of chewing, swallowing and speech.

scholarly journals Quality of Life in Children with Asthma versus Healthy Children

2018 ◽  
Vol 6 (8) ◽  
pp. 1413-1418 ◽  
Author(s):  
Samaneh Kouzegaran ◽  
Parisa Samimi ◽  
Hamid Ahanchian ◽  
Maryam Khoshkhui ◽  
Fatemeh Behmanesh
Keyword(s):  
Quality Of Life ◽  
Physical Performance ◽  
Control Group ◽  
P Value ◽  
Healthy Children ◽  
Significant Difference ◽  
Children With Asthma ◽  
And Performance ◽  
Asthma Group

BACKGROUND: Asthma is the most prevalent chronic disease in the pediatric age group. The disease affects different aspects of the children's lives, such as physical, emotional, social and educational aspects. Thus, more focus has been on the quality of life in these patients rather than the duration of their illness in recent years.AIM: This study examined the different aspects of quality of life in asthmatic children for the first time in this geographic area.METHODS: The study was cross-sectional conducted in 2015-2016. The asthmatic group was 100 patients aged 8 to 12 admitted to the Asthma and Allergy Clinic of Ghaem Hospital (as) in Mashhad with the control group composed of 100 healthy children of the same age and gender. The standard questionnaire pedsQLTM was used for comparing the quality of life of children in the two groups. Statistical analysis was SPSS23 with P-value less than 0.05, which was statistically significant.RESULTS: In each group, 58 patients were boys, and 42 were girls. In a comparison of the quality of life of children, the asthma group with a mean total score of Peds QL 20.99 ± 12.54 compared to the healthy children with a mean total score of Peds QL of 8.8 ± 5.41 had a lower quality of life (P < 0.001). Moreover, regarding various aspects of quality of life asthma group had a lower quality of life in physical performance, emotional performance and performance in school (P < 0.001). Nonetheless, there was no significant difference between the two groups considering social function (P = 0.267). Examining the relationship between Peds QL score of patients with asthma with various variables was indicative of the fact that Peds QL scores were significantly correlated with the gender of the patients, showing better quality of life in the girls (P = 0.001).CONCLUSION: The results indicated that children with asthma have a significantly lower quality of life compared with healthy children of the same age. Also, in examining the different aspects of quality of life, these children had a lower quality of life in physical performance, emotional performance, and performance at school, and were at the level as that of healthy children only in social performance.

scholarly journals Relationship between montelukast and behavioral problems in preschool children with asthma

2022 ◽  
Vol 50 (1) ◽  
pp. 85-91
Author(s):  
Eda Özata ◽  
Zülfikar Akelma ◽  
Sacit Günbey
Keyword(s):  
Preschool Children ◽  
Behavioral Problems ◽  
Inhaled Corticosteroids ◽  
Controlled Study ◽  
Control Group ◽  
Healthy Children ◽  
Significant Difference ◽  
Children With Asthma ◽  
Healthy Control ◽  
Asthma Group

Background: There is insufficient clarity regarding whether or not drugs used in asthma cause behavioral problems in children.Methods: A total of 155 individuals, categorized into an asthma group (n = 95) and a control group (n = 60), were enrolled in the current prospective controlled study. The asthma group consisted of patients receiving treatment (inhaled corticosteroids [ICS] or montelukast) for at least 1 month. Check Behavior Checklist (CBCL) for ages 1.5–5 scores for the asthma and controls were compared. The asthma group was divided into two subgroups based on prophylactic therapy received, ICS and montelukast, and these groups’ CBCL scores were also compared. Results: The asthma group consisted of 95 children (ICS subgroup 45, montelukast subgroup 50) and the healthy control group of 60 cases. The mean total CBCL score was higher in the asthma group than in the control group (42 vs 32, respectively, P = 0.001). Internalization and externalization scores were also higher in the asthma group compared to the control group (P = 0.004 and P = 0.005, respectively). No significant difference was determined in terms of CBCL scores between the ICS and montelukast groups (P = 0.3). Montelukast was discontinued in one asthmatic child due to hallucination.Conclusion: This study determined a higher rate of behavioral problems in preschool children with asthma compared to healthy children. In contrast to other studies in the literature, we determined no difference in terms of total CBCL, and internalization and externalization scores of children with asthma who received ICS and montelukast. Nevertheless, it should be kept in mind that montelukast may cause serious neuropsychiatric events such as hallucination.

scholarly journals Shashen-Maidong Decoction-Mediated IFN-γ and IL-4 on the Regulation of Th1/Th2 Imbalance in RP Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Yuan Yang ◽  
Yanping Zhou
Keyword(s):  
Lavage Fluid ◽  
Interleukin 4 ◽  
Control Group ◽  
Related Factor ◽  
Sprague Dawley ◽  
Relative Ratio ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group D

Objective. Studying correlative changes of Th1/Th2 (Th, Helper T cells) related factor Interferon-γ (IFN-γ) and Interleukin-4 (IL-4) in the progression of radiation pneumonia (RP) rats and the efficacy of Shashen-Maidong decoction on these indexes to explore the immune mechanism of the decoction on the prevention and treatment of RP. Methods. Male 60 Sprague-Dawley (SD) rats were randomly divided into four groups. In addition to the normal control group taking saline, the other rats were set up RP model treated with Shashen-Maidong decoction or dexamethasone (DXM), respectively. The IFN-γ and IL-4 concentrations in serum and bronchoalveolar lavage fluid (BALF) of rats were tested in the 2nd and 4th week after radiation, and the relative ratio of IFN-γ/IL-4 was calculated. Results. (1) There was significant difference of serum IL-4 concentrations in group B (p<0.01) and extreme difference in groups C and D (p<0.001) compared with group A in 4th week. Compared with group D, IL-4 concentrations in group B increased significantly in both 2nd and 4th week (p<0.01). Group B had significantly decreased IFN-γ concentrations in BALF (p<0.001) compared with group D in the 4th week. And IFN-γ concentrations in BALF in group B were increased compared with group C in the 4th week (p<0.05). (2) There was no difference of the relative ratio of IFN-γ/IL-4 at each time in groups B and A for both serum and BALF, while the ratios in groups C and D in 4th week in BALF were increased (p<0.05) compared to group A. Conclusion. Shashen-Maidong decoction can improve the immune function of RP rats by increasing IFN-γ concentration and decreasing IL-4 concentration, possibly by increasing the relative ratio of IFN-γ/IL-4 to regulate the immune imbalance of Th1/Th2.

Comparative Analysis on Family Environment Factors in Children with Asthma and Healthy Children

2014 ◽  
Vol 675-677 ◽  
pp. 245-248
Author(s):  
Ji Hong Wang ◽  
Cai Xia Wang
Keyword(s):  
Environmental Factors ◽  
Family Environment ◽  
Psychological Intervention ◽  
Control Group ◽  
Healthy Children ◽  
The Family ◽  
Children With Asthma ◽  
Care Family ◽  
Group A ◽  
The Impact

The impact of family environmental factors on children with asthma was studied by comparatively analyzing effects of family environmental factors on children with asthma and healthy children to provide a feasible basis for the psychological care. Family Environment Scale Chinese Version (FES-CV) was used conduct a questionnaire on the family environment of 40 children with asthma and 40 healthy children. Scores of the cohesion, emotional expression, successness, entertainment and organization of children in the asthma group were significantly lower than those in the control group, while scores of contradiction were higher than those in the control group. A relatively poor family environment could affect more in children with asthma compared with healthy children, suggesting that that more attention should be paid to the improvement of children’s families and a proper targeted psychological intervention should be given to the children.

scholarly journals The Effect of Curcuma longa on Inflammatory Mediators and Immunological, Oxidant, and Antioxidant Biomarkers in Asthmatic Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Mohammad Hossein Boskabady ◽  
Fatemeh Amin ◽  
Farzaneh Shakeri
Keyword(s):  
Bronchoalveolar Lavage Fluid ◽  
Immunoglobulin E ◽  
Curcuma Longa ◽  
Lavage Fluid ◽  
Interleukin 4 ◽  
Control Group ◽  
Ifn Γ ◽  
Wbc Count ◽  
Asthma Group ◽  
Eosinophil Counts

The effects of Curcuma longa (C. longa) on total and differential WBC, inflammatory and immunologic mediators, and oxidant and antioxidant biomarkers in bronchoalveolar lavage fluid (BALF) of rats model of asthma were assessed. Animals were divided to 5 groups including control (C), asthma (sensitized to ovalbumin), and asthmatic groups treated with 0.75, 1.50, and 3.00 mg/ml C. longa (CL) and 1.25 μg/ml dexamethasone (D) (8 rats in each group). Total and differential WBC count, concentrations of phospholipase A2 (PLA2), total protein (TP), interferon-gamma (IFN-γ), interleukin-4 (IL-4), immunoglobulin E (IgE), NO2, NO3, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and thiol in BALF were assessed. Total and most differential WBC counts and BALF levels of PLA2, TP, IgE, IL-4, and oxidants in asthma group were higher but antioxidants and IFN-γ levels as well as IFN-γ/IL-4 ratio were lower than control group ( p  < 0.001 for all cases). Total WBC and levels of PLA2, IgE, NO2, and NO3 were significantly reduced following treatment with C. longa, compared to asthma group ( p  < 0.001 for all cases). In groups treated with dexamethasone and two higher concentrations of C. longa, neutrophil and eosinophil counts as well as TP, IL-4, and MDA levels were significantly decreased but IFN-γ, IFN-γ/IL-4 ratio, and antioxidants were increased (except IFN-γ/IL-4 ratio), compared to asthma group ( p  < 0.05 to p  < 0.001). Compared to dexamethasone, C. longa exerted more pronounced effects on lung inflammation, oxidative stress, and immune system in asthmatic rats.

scholarly journals Evaluation of neuropsychiatric comorbidities and their clinical characteristics in Chinese children with asthma using the MINI kid tool

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hao Zhou ◽  
Zhihe Chen ◽  
Weiqing Zhao ◽  
Ye Liu ◽  
Yuxia Cui
Keyword(s):  
Mental Health ◽  
Anxiety Disorder ◽  
Clinical Characteristics ◽  
Health Conditions ◽  
Control Group ◽  
Asthmatic Children ◽  
Mental Health Conditions ◽  
Children With Asthma ◽  
Neuropsychiatric Conditions ◽  
Asthma Group

Abstract Background The mental health and quality of life in children with asthma have attracted widespread attention. This study focused on the evaluation of mental health conditions and their clinical characteristics in Chinese children with asthma. Methods A total of 261 children with asthma aged 6 to 16 years old and 261 age- and gender-matched children from the general population were recruited to participate in this study from Guizhou Provincial People’s Hospital. The parents of all subjects were interviewed using the MINI Kid and were required to finish a clinical characteristics questionnaire. Logistic regression analysis was performed to evaluate risk factors. Results The prevalence of mental health conditions in the asthma group was significantly higher than that in the control group (26.4% vs 14.6%, P < 0.001). A total of 10 mental health conditions was identified in the asthma group, the most common of which was ADHD (11.5%; 30/261), followed by oppositional defiant disorder (ODD) (10.7%; 28/261), separation anxiety disorder (6.1%; 16/261), social anxiety disorder (3.8%; 10/261), specific phobias (2.3%; 6/261), agoraphobia without panic (1.5%; 4/261), (mild) manic episodes (1.1%; 3/261), major depressive episodes (MDEs) (0.8%; 2/261), movement (tic) disorder (0.8%; 2/261), and dysthymia (0.4%; 1/261). A total of 6 neuropsychiatric conditions was detected in the control group, including ODD (5.7%; 15/261), ADHD (4.6%; 12/261), social anxiety disorder (3.1%; 8/261), seasonal anxiety disorder (SAD) (2.3%; 6/261), specific phobias (1.1%; 3/261), and agoraphobia without panic (0.4%; 1/261). The prevalence rates of ODD, ADHD, and SAD differed significantly between the two groups (P < 0.05). Multiple regression analysis revealed that severe persistent asthma (OR = 3.077, 95% CI 1.286–7.361), poor asthma control (OR = 2.005, 95% CI 1.111–3.619), and having asthma for > 3 years (OR = 2.948, 95% CI 1.580–5.502) were independent risk factors for the presence of mental health conditions in asthmatic children. Conclusions Children with asthma have a higher rate of mental health conditions than non-asthmatic children. Standardized diagnosis and treatment may help reduce the risk of neuropsychiatric conditions.

scholarly journals Anti-inflammatory effect of wogonin on allergic responses in ovalbumin-induced allergic rhinitis in the mouse

2018 ◽  
Vol 9 ◽  
pp. 215265671876414 ◽  
Author(s):  
Kyeong Ah Kim ◽  
Joo Hyun Jung ◽  
Yun Sook Choi ◽  
Gyu Kang ◽  
Seon Tae Kim
Keyword(s):  
Allergic Rhinitis ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Serum Levels ◽  
Lavage Fluid ◽  
Specific Ige ◽  
Eosinophil Infiltration ◽  
Treatment Groups ◽  
Specific Immunoglobulin E ◽  
Group A

Background Wogonin is commonly used for the treatment of allergic diseases. However, neither its precise effect in preventing allergic rhinitis (AR) nor its mechanism of action are known. Objectives In this study, the effect of wogonin on allergic responses in ovalbumin (OVA) induced AR was investigated in mice. Methods BALB/c mice were sensitized with intraperitoneal (i.p.) OVA and then challenged intranasally with OVA. Wogonin (10 and 30 mg/kg) was given to the treatment groups, and the effect of wogonin on the release of allergic inflammatory mediators, specifically OVA-specific immunoglobulin E (IgE) and inflammatory cytokines, was explored. Eosinophil infiltration and the levels of interleukin (IL) 5 and IL-13 were measured by immunohistochemistry. Results In mice with AR, wogonin decreased OVA-specific IgE levels in serum, and the levels of the cytokines IL-4, IL-5, IL-13, eotaxin, and RANTES in nasal lavage fluid. Serum levels of IL-4, IL-5, and IL-13 were lower in both groups of wogonin-pretreated mice than in the OVA group. A reduction in eosinophil infiltration of the nasal mucosa and inhibition of the expression of IL-5 and IL-13 were also noted in the treated groups. Conclusion Wogonin induced antiallergic effects in a murine model of AR by decreasing the infiltration of eosinophils and levels of T-helper type 2 cytokines. Thus, wogonin merits consideration as a therapeutic agent for treating AR.

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