scholarly journals Royal jelly and propolis therapies reduce inflammation and stimulate healing of oral mucositis in rats

2021 ◽  
Author(s):  
Mara Luana Batista Severo ◽  
Stéfanie Thieme ◽  
Felipe Martins Silveira ◽  
Raquel Padilha Martins Tavares ◽  
Amanda Katarinny Goes Gonzaga ◽  
...  
Keyword(s):  
Oral Mucositis ◽  
Wistar Rats ◽  
Royal Jelly ◽  
Mechanisms Of Action ◽  
Phytochemical Analysis ◽  
Stress Markers ◽  
Male Wistar Rats ◽  
Layer Chromatography ◽  
And Oxidative Stress ◽  
Wound Healing Activity

Abstract Purpose This study aimed to evaluate the effects and mechanisms of action of royal jelly (RJ) and propolis in an animal model of oral mucositis (OM).Methods Seventy-two male Wistar rats were randomly allocated to four groups (n = 18): control (no treatment), PBMT (intraoral laser, 6 J/cm2), RJ, and propolis. On days 0 and 2, animals received injection of 5-fluorouracil (5-FU). The buccal mucosa was scratched (days 3 and 4) and the treatments were initiated on day 5. Six animals of each group were euthanized on days 8, 10, and 14. Phytochemical analysis (thin-layer chromatography-TLC), clinical, histopathological, immunohistochemical of pS6, pAKT and NF-κB and oxidative stress markers were also investigated.Results TLC revealed the presence of large amounts of sucrose (Rf 0.34) in RJ and flavonoids in propolis. On day 8, lower clinical OM scores (and day 10) and morphological were observed in the PBMT, RJ and propolis groups (p < 0.05). On day 8, immunoexpression of pS6, pAKT and NF-κB was increased when compared to control. On day 14, GSH antioxidant levels were increased in the propolis group compared to control (p < 0.05).Conclusions Our results showed that RJ and propolis, as well as PBMT, are effective in the treatment of OM. The RJ and propolis may explain their excellent wound healing activity and anti-inflammatory effects. Considering that not all patients who develop OM have access to PBMT, the present study demonstrated that topical application of RJ and propolis may be an important alternative for the treatment of OM.

scholarly journals Effects of Leaf Extract of Cnidoscolus aconitifolius on Serum Lipids and Oxidative Stress Markers of Male Wistar Rats

2020 ◽  
pp. 47-52
Author(s):  
Ijeoma Ezebuiro ◽  
Chibuike Obiandu ◽  
Friday Saronee ◽  
Adesua C. Obiandu
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Cnidoscolus Aconitifolius ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
And Oxidative Stress

Introduction: Cnidoscolus aconitifolius is considered to be an important and effective medicinal plant in folklore remedies where it has been applied as an alternative therapy for the treatment of various ailments. Aim: The present study aims to determine the effects of Cnidoscolus aconitifolius on lipid profile and some oxidative stress markers of male Wistar rats. Methodology: A total of 15 male wistar rats were procured for the study and randomly assigned into three groups of 5 rats each. Group 1 served as control and received distilled water only. Group 2 received 200 mg/kg and group 3 received 400mg/kg of the hydromethanolic (1:4) extract of Cnidoscolus aconitifolius which was administered as single daily dose using oral cannula. On completion of treatment, blood samples were collected by cardiac puncture for determination of some serum lipid parameters and oxidative stress markers. Results: Results showed that there were no significant difference in the serum level of total cholesterol, triglyceride, low density lipoprotein cholesterol and high density lipoprotein cholesterol at both doses of the extract, compared to control. However, compared to control, there was a significant (p<0.05) increase in the activity of superoxide dismutase and glutathione reductase but significant reduction in malondialdehyde level. The catalase enzyme activity was not significant. Conclusion: The result obtained suggest that the extract may be useful in reducing oxidative stress by improving some antioxidant enzyme activities and may also prevent cell death due to lipid peroxidation.

scholarly journals The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

2014 ◽  
Vol 66 (3) ◽  
pp. 1075-1081
Author(s):  
Ivan Simic ◽  
Violeta Iric-Cupic ◽  
Rada Vucic ◽  
Marina Petrovic ◽  
Violeta Mladenovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Wistar Rats ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

scholarly journals Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

2020 ◽  
Vol 80 (1) ◽  
pp. 12-22 ◽  
Author(s):  
F. Carmignan ◽  
R. Matias ◽  
C. A. Carollo ◽  
D. M. Dourado ◽  
M. H. Fermiano ◽  
...  
Keyword(s):  
Wistar Rats ◽  
High Efficiency ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Skin Lesions ◽  
Phytochemical Analysis ◽  
Cutaneous Wounds ◽  
Tissue Restoration ◽  
Layer Chromatography ◽  
Flavonoid Patterns

Abstract Medicinal plants, such as E. pyramidale, are alternatives against infections and assist in the healing process of wounds. We evaluated the effects of Hydrogel of E. pyramidale on the healing of cutaneous wounds in animal models by morphological, morphometric and histological analyses, and elucidated major components. The ethanolic extract of E. pyramidale shoots was submitted to phytochemical analysis. For cicatrizing activity assay, Wistar rats were used, and the groups evaluated were hydrogel containing ethanolic extract of E. pyramidale at 2% compared to control groups. The treatment periods were 3, 7 and 21 days, and macroscopic and microscopic analyses were performed. Data were submitted to Analysis of Variance (p <0.05). Phytochemistry and quantification evidenced that flavonoids are main constituents and that they were evidenced by thin layer chromatography (TLC), high efficiency (HPLC) and infrared (IR) methods. In TLC, using flavonoid patterns, we observed pinocembrin. The hydrogel containing ethanolic extract of E. pyramidale at 2% was effective in wound regression. E. pyramidale can be used for the treatment of second intention wounds, and the effective healing may be due to a high flavonoid content.

Honey prevents neurobehavioural deficit and oxidative stress induced by lead acetate exposure in male wistar rats- a preliminary study

2015 ◽  
Vol 31 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Wahab Imam Abdulmajeed ◽  
Habeeb Bolakale Sulieman ◽  
Maymunah Oloruntosin Zubayr ◽  
Aminu Imam ◽  
Abdulbasit Amin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Male Wistar Rats ◽  
Preliminary Study ◽  
And Oxidative Stress

scholarly journals Riboceine Regimen Attenuates Ethanol-induced Neuronal Damage in the Cerebellum of Adult Male Wistar Rats

2021 ◽  
Vol 15 (4) ◽  
pp. 249-256
Author(s):  
Taiwo Adekemi Abayomi ◽  
◽  
Olorunfemi Samuel Tokunbo ◽  
Moyinoluwa Ajayi ◽  
Olawale Ayobami Abayomi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Neuronal Damage ◽  
Motor Impairment ◽  
Stress Profile ◽  
Stress Markers ◽  
Ethanol Toxicity ◽  
Male Wistar Rats ◽  
Cerebellar Astrocytes ◽  
The Brain

Background: Although ethanol exerts its neurotoxic effect on the brain through inflammatory and oxidative processes, the effect of Riboceine on the brain following ethanol neurotoxicity is yet to be elucidated. Therefore, this study was designed to evaluate the effects of riboceine on the cellular, behavioral, and molecular impairments induced by ethanol toxicity in rats. Methods: A total of 24 male Wistar rats weighing between 160-170 grams were used for the study, and were divided into four groups of six rats each. After completion of the administration of ethanol and riboceine, and testing for motor impairment, the rats were sacrificed. The cerebellum was excised and processed for oxidative stress analyses, based on oxidative stress markers and histological examinations. The immunohistochemical expression of astrocytes in the cerebellum was examined, using Glial Fibrillary Acidic Protein (GFAP) stain. Results: This study demonstrated that ethanol-induced neurotoxicity in the cerebellum, characterized by increased oxidative stress profile, astrocyte activation, and neuronal death in the cerebellum, especially the Purkinje layer. Necrosis, significant decrease in Superoxide Dismutase (SOD), Catalase (CAT) and Gluathione (GSH) activities (P<0.05) as well as astrogliosis was associated with ethanol treatment. However, riboceine was observed to significantly increase the cerebellar SOD, CAT and GSH activities with significantly reduced Malondialdehyde (MDA) levels (P<0.05). It also attenuated the histomorphological alteration of the cerebellum and reduced the cerebellar astrocytes activation following ethanol-induced neurotoxicity, thus leading to the attenuation of motor impairment. Conclusion: Riboceine attenuated motor impairment caused by chronic ethanol-induced neurotoxicity, suggestive of its anti-oxidative and anti-inflammatory properties.

scholarly journals Burn Wound Healing Activity of Prunella Grandiflora L. Exctract

Biomeditsina ◽  
2021 ◽  
Vol 17 (4) ◽  
pp. 50-56
Author(s):  
E. V. Bolotnik ◽  
L. I. Alekseeva ◽  
L. P. Larionov
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Dosage Forms ◽  
Control Groups ◽  
Burn Wound ◽  
Dry Extract ◽  
Burn Wound Healing ◽  
Male Wistar Rats ◽  
And Control ◽  
Wound Healing Activity

The qualitative composition of phenolic acids and burn wound healing activity of Prunella grandiflora L. extract were studied. The content of rosmarinic (621±5 mg/g), coffeic (11.2±0.2 mg/g), syringic (46.5±1.4 mg/g), n-coumaric (25.8±0.5 mg/g), and ferulic (42.7±2.9 mg/g) acids in dry extracts of P. grandiflora was determined by HPLC. Three new burn-healing agents of different composition containing 5% of the dry extract of Prunella grandiflora L. (big-flowered self-heal) were developed. The developed agents were tested on male Wistar rats with second- and third-degree thermal burns. An assessment of skin restoration was carried out in experimental and control groups of animals. It was revealed that animals treated with the developed soft dosage forms restored the epithelium and coat 6–8 days faster that those treated with compositions containing no extract under study. It was found that the developed soft dosage forms based on P. grandiflora extract exhibit anti-inflammatory and wound-healing action.

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