scholarly journals Association mapping of seed vigor in spring soybean (Glycine max (L.) Merr.) in northeast China

2021 ◽  
Author(s):  
Caijin Wang ◽  
Huiyan Zhao ◽  
Yang Wang
Keyword(s):  
Association Mapping ◽  
Ssr Markers ◽  
Northeast China ◽  
Soybean Seed ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Hypocotyl Length ◽  
Wide Range ◽  
Carrier Materials ◽  
Normal Seedling

Abstract High-vigor soybean seeds can result in the rapid and uniform emergence of seeds under a wide range of field conditions. We aimed to explore the quantitative trait loci of seed vigor traits and superior allelic and carrier materials from the soybean in northeast China. A total of 257 improved lines and 104 soybean landraces were used to evaluate seed vigor, using normal seedling fresh weight, normal seedling length, main root length, hypocotyl length, and germination percentage, in 2018 and 2019. In this study, the different degrees of LD was detected not only among syntenic markers but also among nonsyntenic ones, suggesting that there had been historical recombination among linkage groups. A total of 19 and 10 simple sequence repeat (SSR) markers associated with seed vigor were detected in improved lines and soybean landraces in 2018 and 2019, respectively. Our results also showed five SSR markers co-associated with two or three vigor traits. In addition, 41 superior alleles and their carrier materials were mined. Based on the elite alleles detected, the best cross combinations for improving seed vigor traits were proposed. The results demonstrate that association mapping is a valuable foundation for molecular breeding for soybean seed vigor.

scholarly journals Pengaruh priming terhadap vigor benih kedelai (Glycine max (L.) Merril.) yang dikecambahkan pada media dengan cekaman alumunium

Jurnal Agro ◽  
2022 ◽  
Vol 8 (2) ◽  
pp. 178-187
Author(s):  
Agustiansyah Agustiansyah ◽  
Paul B Timotiwu ◽  
Nabila Lutfiah
Keyword(s):  
Seed Germination ◽  
Soybean Seed ◽  
Acid Soils ◽  
Seed Vigor ◽  
Aluminium Content ◽  
Hypocotyl Length ◽  
Peg 6000 ◽  
Randomized Design ◽  
High Aluminium ◽  
Improve Seed Germination

Kandungan aluminium yang tinggi pada tanah Ultisol merupakan salah satu masalah utama dalam budidaya tanaman kedelai mulai dari fase perkecambahan hingga pertumbuhan tanaman. Perlakuan priming pada benih diyakini mampu memperbaiki perkecambahan pada lingkungan yang kurang menguntungkan. Penelitian dilakukan untuk mengetahui pengaruh priming pada perkecambahan benih kedelai yang dikecambahkan dalam media masam dan mengandung aluminium. Penelitian disusun dalam Rancangan Acak Lengkap (RAL) pola faktorial. Faktor pertama adalah jenis priming : tanpa priming, H2O (air), KNO3 1%, KNO3 2%, GA3 50 ppm, GA3 100 ppm, dan PEG 6000 7,5%. Faktor kedua adalah varietas kedelai yaitu: Anjasmoro, Burangrang, dan Grobogan. Data dianalisis sidik ragamnya dan dilanjutkan dengan uji Beda Nyata Jujur (BNJ) 5% menggunakan program statistika R Studio. Hasil penelitian menunjukkan priming PEG 7,5% dan varietas Burangrang secara mandiri merupakan perlakuan terbaik untuk meningkatkan vigor benih kedelai berdasarkan variabel waktu muncul kecambah, kecepatan perkecambahan, dan panjang hipokotil. Implikasi penelitian ini adalah PEG 7,5% dapat digunakan untuk perbaikan perkecambahan pada tanah masam dengan kandungan aluminium yang relatif tinggi. High aluminium content in Ultisol is the main problem in soybean cultivation from germination to planting growth phases.Priming treatment is believed to improve seed germination in a poor-growing environment. This study aimed to determine the effect of priming of soybean seeds germinated under acid medium and aluminium stress. The experimental design used was Completely Randomized Design (CRD) with factorial pattern. The first factor was priming type : no priming, H2O (water), 1% KNO3, 2% KNO3, 50 ppm GA3, 100 ppm GA3, and 7.5% PEG 6000. The second factor was soybean variety : Anjasmoro, Burangrang, and Grobogan.  The data obtained were analyzed using the R Studio statistic program with a 5% HSD test. The study resulted that each PEG 7.5% priming and Burangrang  variety independently was the best in increasing soybean seed vigor based on sprout time emergence, germination speed and hypocotyl length. This research implicates that PEG 7.5% can be used to improve seed germination on acid soils with high aluminium content. 

scholarly journals Correlation between vigor by accelerated aging at pre-sowing and soybean seedling emergence in the field

2021 ◽  
Vol 42 (2) ◽  
pp. 455-470
Author(s):  
Vanderléia Mathias ◽  
◽  
Cileide Maria Medeiros Coelho ◽  
Keyword(s):  
Exposure Time ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Growing Seasons ◽  
Accelerated Aging Test ◽  
Physiological Quality ◽  
Field Emergence

Although the accelerated aging test is widely used to determine seed vigor, there are methodological variations in seed exposure time at 41 °C. Therefore, the objective was to indicate the most appropriate seed exposure time to segregate lots that also exhibits a correlation with seedling emergence in the field, and establish vigor levels using the aforementioned test. Soybean seed samples were collected at pre-sowing, representing 100 lots in the 2016/2017 growing season and 125 lots in the 2017/2018 season from different warehouses in 4 regions of Santa Catarina state (SC). Physiological quality was evaluated by germination, accelerated aging (24 and 48 h at 41 °C) and field emergence tests. The results were submitted to descriptive statistical analysis and Pearson’s correlation analysis (p < 0.05). The rank sum index, associated with the Scott-Knott clustering method, was used to classify physiological quality. The average germination percentage was 90 and 91% in the 2016/2017 and 2017/2018 growing seasons, respectively. Vigor obtained an average of 86 (2016/2017) and 85% (2017/2018), when seeds were submitted to accelerated aging for 48 h, and 89% (both seasons) for 24 h. In regard to field emergence, the average was 85 and 80% in the 2016/2017 and 2017/2018 growing seasons, respectively. Considering the 225 lots, the greatest correlation with field emergence was observed in vigor - 48 h (r = 0.71 p < 0.05). Rank sum classification made it possible to establish vigor-based categories, whereby batches with over 85% vigor by 48-h accelerated aging were classified as high vigor, 70 to 84% medium vigor, and under 70% low vigor.

scholarly journals Explanining the vigor (relative and absolute) in seeds: the biological meaning behind the mathematician

2020 ◽  
Vol 36 ◽  
Author(s):  
Vanderley José Pereira
Keyword(s):  
Review Article ◽  
Seed Vigor ◽  
Mathematical Basis ◽  
Biological Meaning ◽  
Wide Range ◽  
Normal Seedling ◽  
Physiological Quality ◽  
New Interpretation ◽  
Viable Seeds

Seed vigor is the totality of all properties that determine a rapid and uniform emergence and development of normal seedling under a wide range of conditions. However, the physiological quality within a seed lot is not  homogeneous, generating a quality gradient between seeds. Thus, the vigor expressed by the final percentage of normal seedlings tends to underestimate the quality of the batch, considering the total number of seeds. One possible method for correcting such an effect would be to weight vigor by germination, generating an index called relative vigor. The index reflects the “success” of viable seeds in maintaining their potential under stress. In this context, this review article proposes the possibility of using a new measure for vigor and new interpretation of relative vigor, as well as providing the mathematical basis for its use.

scholarly journals Biochemical changes stimulated by accelerated aging in safflower seeds (Carthamus tinctorius L.)

2020 ◽  
Vol 42 ◽  
Author(s):  
Sercan Önder ◽  
Muhammet Tonguç ◽  
Damla Güvercin ◽  
Yaşar Karakurt
Keyword(s):  
Soluble Sugars ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Carthamus Tinctorius ◽  
Tocopherol Content ◽  
Seed Vigor ◽  
Biochemical Changes ◽  
Physiological Basis ◽  
Germination Ability ◽  
Normal Seedling

Abstract: Seed vigor tests are used to estimate their quality. One of the most commonly used is the accelerated aging test (AA). The aim of the present study was to study the biochemical changes caused in the seeds and to determine their germination status after the AA. Six safflower genotypes were tested at 43 °C and 45 °C for 0, 48, 72, 96 and 120 h, and germination percentage (GP), mean germination time (MGT) and normal seedling percentage (NSP) were evaluated to determine the aging reactions of the genotypes. During the AA at 45 °C, the seeds quickly lost their germination ability after 48 h; after 120 h, the seeds lost their viability, remaining, however, still viable at 43 °C. Two genotypes that aged more (Linas and Olas) and less (Bayer-6 and Bayer-12) were chosen to examine the biochemical changes during the AA at 43 °C. Eleven biochemical analysis were performed to understand physiological changes associated with the test. Total caratone, xanthophyll, phenolics, flavonoid, soluble protein, soluble sugars, oil and malondialdehyde contents were lower after 120 h, compared to 0 h. Reducing sugars and free fatty acids contents increased in the least and most aging genotypes. However, the total tocopherol content increased in the least aging genotypes and decreased in the most aging genotypes after 120 h, compared to 0 h. The results showed that the AA at 43 °C was suitable to study the aging process in the safflower seeds. Besides, understanding the chemical changes was useful to elucidate the physiological basis of seed aging.

Use of tall fescue EST-SSR markers in phylogenetic analysis of cool-season forage grasses

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 637-647 ◽  
Author(s):  
M A. Rouf Mian ◽  
Malay C Saha ◽  
Andrew A Hopkins ◽  
Zeng-Yu Wang
Keyword(s):  
Phylogenetic Analysis ◽  
Ssr Markers ◽  
Tall Fescue ◽  
Related Species ◽  
Grass Species ◽  
Forage Grass ◽  
Forage Grasses ◽  
Cool Season ◽  
Phylogenetic Relations ◽  
Wide Range

Microsatellites or simple sequence repeats (SSRs) are highly useful molecular markers for plant improvement. Expressed sequence tag (EST)-SSR markers have a higher rate of transferability across species than genomic SSR markers and are thus well suited for application in cross-species phylogenetic studies. Our objectives were to examine the amplification of tall fescue EST-SSR markers in 12 grass species representing 8 genera of 4 tribes from 2 subfamilies of Poaceae and the applicability of these markers for phylogenetic analysis of grass species. About 43% of the 145 EST-SSR primer pairs produced PCR bands in all 12 grass species and had high levels of polymorphism in all forage grasses studied. Thus, these markers will be useful in a variety of forage grass species, including the ones tested in this study. SSR marker data were useful in grouping genotypes within each species. Lolium temulentum, a potential model species for cool-season forage grasses, showed a close relation with the major Festuca–Lolium species in the study. Tall wheatgrass was found to be closely related to hexaploid wheat, thereby confirming the known taxonomic relations between these species. While clustering of closely related species was found, the effectiveness of such data in evaluating distantly related species needs further investigations. The phylogenetic trees based on DNA sequences of selected SSR bands were in agreement with the phylogenetic relations based on length polymorphism of SSRs markers. Tall fescue EST-SSR markers depicted phylogenetic relations among a wide range of cool-season forage grass species and thus are an important resource for researchers working with such grass species.Key words: phylogeny, EST-SSR, forage grasses, tall fescue.

Factors affecting red-osier dogwood seed germination

1992 ◽  
Vol 70 (5) ◽  
pp. 1012-1016 ◽  
Author(s):  
S. N. Acharya ◽  
C. B. Chu ◽  
R. Hermesh ◽  
G. B. Schaalje
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Collection Site ◽  
Moisture Condition ◽  
Seed Formation ◽  
Factors Affecting ◽  
Year Effect ◽  
Wide Range ◽  
Cornus Stolonifera ◽  
Moisture Conditions

Seeds from 55 populations of native Alberta red-osier dogwood (Cornus stolonifera Michx.) were collected over 2 years (1984 and 1985) to determine the range of variability for seed germination and the effect of ecoregion and moisture conditions in the collection sites on this trait. Each year the unscarified seeds were stored at 3 °C for 30 days, stratified at 3 °C for an additional 30 days and then incubated on an 8 h light: 16 h dark cycle at a temperature of 25:10 °C (light:dark) for germination. A wide range of germination percentages was observed among populations collected in any 1 year. However, the variability was not due to the ecoregion or moisture condition of the collection site. Germination percentage of populations collected in 1984 and 1985 formed five and seven groups, respectively. A study involving eight sites that were common to 1984, 1985, and 1986 revealed a significant effect of population on seed germination. This population effect was not obscured by the combined effects of year, precipitation, temperature, and seed size. The year effect was significant only when population was used as a covariate. Precipitation during seed formation significantly influenced germination performance even after adjustments were made for population and year. Seed germination in red-osier dogwood appears to be influenced by the population from which the seeds are collected and by yearly environmental fluctuations. Therefore, germination studies in this species must include seeds from diverse populations collected over several years. Key words: red-osier dogwood, Cornus stolonifera Michx., germination, population, environment.

scholarly journals Genetic diversity in soybean seed quality under different storage conditions

2017 ◽  
Vol 38 (1) ◽  
pp. 57
Author(s):  
Daniele Piano Rosa ◽  
Danúbia Aparecida Costa Nobre ◽  
Diego Santos Oliveira ◽  
Francisco Charles dos Santos Silva ◽  
André Ricardo Gomes Bezerra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Quality ◽  
Soybean Seed ◽  
High Standard ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Soybean Cultivars ◽  
High Germination ◽  
Physiological Quality ◽  
Almost All

This study aimed to assess the effect of genetic diversity on physiological quality of soybean seeds stored in cold chamber and under environmental conditions. Ten cultivars were assessed in a randomized factorial design (2x10). Factor 1 corresponded to two storage conditions and factor 2 to ten soybean cultivars, with four replications. The evaluated variables were total germination (G%), first count of germination (F%), percentage of abnormal seedlings (AS%), germination speed index (GSI), water content (WC), electrical conductivity (EC), dry matter of seedlings (DMS) and length of seedlings (LS). Data underwent ANOVA, followed by Scott Knott test, as well as multivariate analysis of genetic diversity. The results showed a higher physiological quality for seeds under cold storage. Half of the cultivars (FPS Júpiter, FPS Urano, FPS Antares, FPS Netuno and CD 250) presented high germination rates and seed vigor, being thus indicated as high-standard materials for further breeding programs. Besides that, storage environment had influence on the clustering of soybean cultivars. Moreover, cultivars had genetic dissimilarity for almost all assessed traits as G%, GSI, F%, AS%, EC, DMS and LS.

scholarly journals Alternative procedure for the cold test for soybean seeds

2010 ◽  
Vol 67 (5) ◽  
pp. 540-545 ◽  
Author(s):  
Bruno Guilherme Torres Licursi Vieira ◽  
Roberval Daiton Vieira ◽  
Francisco Carlos Krzyzanowski ◽  
José de Barros França Neto
Keyword(s):  
Glycine Max ◽  
Seed Quality ◽  
Soybean Seed ◽  
Cold Test ◽  
Seed Vigor ◽  
Alternative Procedure ◽  
Test Procedures ◽  
Paper Towel ◽  
Traditional Procedure ◽  
Seed Crops

The growing demand for high quality soybean [Glycine max (L.) Merrill] seeds requires a precise seed quality control system from the seed industry. One way to accomplish this is by improving vigor testing. Cold test has been traditionally employed for corn seeds. However, it has also been used for other seed crops such as cotton (Gossypium spp.), soybean (Glycine Max), dry bean (Phaseolus vulgaris) and pea (Pisum sativum). This study was carried out with the objective of adjusting an alternative procedure for the cold test to determine soybean seed vigor. Six commercial soybean seed lots of the cultivar BRS 133 were used. The physiological potential of the seed lots was evaluated by germination on paper towel and sand box, seedling field emergence, tetrazolium, accelerated aging and electrical conductivity tests. Seed moisture content was also determined. The temperature used for the cold test procedures was 10ºC during five days. Four cold test procedures were evaluated: i) plastic boxes with soil; ii) rolled paper towel with soil; iii) rolled paper towel without soil, and iv) an alternative procedure, using rolled paper towel without soil under cold water. A completely randomized experimental design with eight replications was used and the means were compared by the Tukey test (p = 0.05). To verify the dependence between the alternative test and others single linear correlation was used. All cold test procedures had similar coefficients of variation (CV), highlighting that rolled paper towel with soil and the alternative procedure had the best performance, with an average of 94% and 93% normal seedlings and CV of 3.2% and 3.6%, respectively. The alternative procedure has satisfactory results for estimating soybean seed vigor, yielding consistent results compared to the traditional procedure.

scholarly journals Electrical conductivity of the seed soaking solution and soybean seedling emergence

2004 ◽  
Vol 61 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Angelo Scappa Neto ◽  
Sonia Regina Mudrovitsch de Bittencourt ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Water Content ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Soybean Seedling ◽  
Seed Soaking ◽  
Laboratory Conditions ◽  
Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

scholarly journals High gamma irradiation doses and long storage times reduce soybean seed quality

2016 ◽  
Vol 37 (3) ◽  
pp. 1219
Author(s):  
Danúbia Aparecida Costa Nobre ◽  
Carlos Sigueyuki Sediyama ◽  
Valter Arthur ◽  
Newton Deniz Piovesan ◽  
Alisson Santos Lopes da Silva
Keyword(s):  
Gamma Irradiation ◽  
Seed Quality ◽  
Germination Rate ◽  
Soybean Seed ◽  
Response Surfaces ◽  
Seed Vigor ◽  
Production Cycle ◽  
High Gamma ◽  
Storage Times ◽  
Long Storage

High quality seeds are required for soybean production. This study evaluated the effect of gamma irradiation and storage time on seed quality in soybean lines VX04-6828 and VX04-5692. Seeds were gamma irradiated (60Co) with 0, 50, 150, and 250 Gy. After the first seed production cycle (M1), the harvested seeds were stored in the laboratory for 0, 2, 4, and 6 months. Moisture content, seed quality (germination rate, dead seeds, and normal and abnormal seedlings), and seed vigor (first germination count, germination index, and seedling length) were determined. Data were submitted to analysis of variance for each soybean line using a 4 x 4 factorial design (four storage times x four gamma irradiation doses). Response surfaces were constructed based on the F test significance (p ? 0.05). VX04-5692 seeds were more sensitive to gamma radiation than were VX04-6828 seeds. Soybean seed quality was highest in M2 seeds derived from seeds irradiated with less than 100 Gy and stored for up to two months. High gamma irradiation doses and long storage times reduced soybean seed quality.

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