Evaluation of skin-surface interleukin 1 alpha, interleukin-1 Receptor Antagonist, CXCL-1/2 and beta-defensin-1 as non-invasive biomarkers for monitoring psoriasis vulgaris

Abstract Background: Psoriatic skin is typically evaluated through visual assessment of its clinical hallmarks: thickness, redness and scaling of skin lesions. Sonography is used to physically monitor disease severity through physical assessment of the skin outer layers. Both methods assess consequences of inflammation rather than the molecular basis of disease. The aim of the current study was to assess 1) whether expression patterns of IL-1α, IL-1RA, CXCL-1, CXCL-2, and hBD-1, examples of proteins known to drive psoriasis pathology, can be measured non-invasively from the skin surface, 2) whether expression patterns of these proteins correlate with disease severity, and 3) whether skin surface expression of these proteins can be used to measure pharmacodynamic effects of psoriasis treatment. Results: Using non-invasive FibroTx TAP technology for sampling and measurements of proteins from the skin of psoriasis patients revealed clear differences of IL-1α, IL-1RA, CXCL-1/2 on lesional skin when compared to non-lesional skin or skin from healthy volunteers. Comparing these expression patterns with visual assessment of thickness, redness and scaling of skin lesions revealed no significant quantitative correlations, with the exception of a weak correlation between CXCL-1/2 and thickness of lesions. Similarly, there were no significant correlations between FibroTx TAP measurements and ultrasound measurements, with the exception of a weak correlation between CXCL-1/2 and SLEB thickness. The potential of these skin-surface biomarkers were studied by monitoring skin lesions of psoriasis patients undergoing narrow-band UVB (311 nm) phototherapy. During the course of UVB treatment, clear patterns towards normalisation of IL-1RA and CXCL-1/2 were observed on lesions measured. Conclusions: Skin surface measurements of proteins involved in psoriasis skin pathology, in this study exemplified by IL-1α, IL-1RA, CXCL-1/2 and hBD-1, have potential as biomarkers for monitoring severity of disease, as well as for monitoring pharmacodynamic changes. Skin surface measurements of IL-1RA and CXCL-1/2 displayed a different profile than achieved by visual scoring of local inflammation or sonography, thus confirming that measuring the ‘molecular root’ of inflammation appears to have value as an objective, non-invasive biomarker measurement for scoring disease severity in its own right.

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Evaluation of skin-surface interleukin 1 α, interleukin-1 Receptor Antagonist, CXCL-1/2 and β-defensin-1 as non-invasive biomarkers for monitoring psoriasis vulgaris

10.21203/rs.3.rs-33637/v2 ◽

2020 ◽

Author(s):

Kadri Orro ◽

Kristiina Salk ◽

Kristi Abram ◽

Jelena Arshavskaja ◽

Anne Meikas ◽

...

Keyword(s):

Disease Severity ◽

Interleukin 1 ◽

Skin Surface ◽

Skin Lesions ◽

Diagnostic Tools ◽

Therapeutic Effects ◽

Lesional Skin ◽

Non Invasive ◽

Surface Measurements ◽

Monitoring Treatment

Abstract There is a need for non-invasive diagnostic tools that can objectively measure psoriasis activity and that can be used to monitor therapeutic effects of psoriasis treatment. This study aimed to determine whether non-invasive measurements of proteins from psoriasis lesional skin can be used to assess disease severity and to measure treatment efficacy.Using FibroTx TAP technology for protein-measurements directly from the surface of skin, clear differences in levels of IL-1α, IL-1RA and CXCL-1/2 were found between psoriasis lesional skin and non-lesional skin. No clear correlations were found between FibroTx TAP measurements and PASI scoring, with the exception of a mild correlation between CXCL-1/2 and scaling. Similarly, no clear correlations were found between FibroTx TAP measurements and ultrasound measurements of skin, with the exception of a weak correlation between IL-1RA and SLEB thickness. Monitoring IL-1RA and CXCL-1/2 on skin lesions undergoing narrow-band UVB phototherapy clearly reflected normalisation of skin.Skin-surface measurements of IL-1RA and CXCL-1/2 have potential for assessing severity of psoriasis and for monitoring treatment efficacy. Measurements of IL-1RA and CXCL-1/2 displayed a disease profile distinct from PASI or sonography, thus confirming that measuring the ‘molecular root’ of inflammation has value for scoring disease severity in its own right.

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Evaluation of skin-surface interleukin 1 α, interleukin-1 Receptor Antagonist, CXCL-1/2 and β-defensin-1 as non-invasive biomarkers for monitoring psoriasis vulgaris

10.21203/rs.3.rs-33637/v3 ◽

2021 ◽

Author(s):

Kadri Orro ◽

Kristiina Salk ◽

Kristi Abram ◽

Jelena Arshavskaja ◽

Anne Meikas ◽

...

Keyword(s):

Disease Severity ◽

Treatment Efficacy ◽

Interleukin 1 ◽

Skin Surface ◽

Skin Lesions ◽

Diagnostic Tools ◽

Therapeutic Effects ◽

Lesional Skin ◽

Non Invasive ◽

Surface Measurements

Abstract Background: There is a need for non-invasive diagnostic tools that can objectively measure psoriasis activity and that can be used to monitor therapeutic effects of psoriasis treatment. This study aimed to determine whether non-invasive measurements of proteins from psoriasis lesional skin can be used to assess disease severity and to measure treatment efficacy. Results: Using FibroTx TAP technology for protein-measurements directly from the surface of skin, clear differences in levels of IL-1a, IL-1RA and CXCL-1/2 were found between psoriasis lesional skin and non-lesional skin. No clear correlations were found between FibroTx TAP measurements and PASI scoring, with the exception of a mild correlation between CXCL-1/2 and scaling. Similarly, no clear correlations were found between FibroTx TAP measurements and ultrasound measurements of skin, with the exception of a weak correlation between IL-1RA and SLEB thickness. Monitoring IL-1RA and CXCL-1/2 on skin lesions undergoing narrow-band UVB phototherapy clearly reflected normalisation of skin. Conclusions: Skin-surface measurements of IL-1RA and CXCL-1/2 have potential for assessing severity of psoriasis and for monitoring treatment efficacy. Measurements of IL-1RA and CXCL-1/2 displayed a disease profile distinct from PASI or sonography, thus confirming that measuring the ‘molecular root’ of inflammation has value for scoring disease severity in its own right.

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Melanoma Screening with Computer Aided Dermoscopy System Based on the ABCD Criteria

Dermatology and Dermatitis ◽

10.31579/2578-8949/052 ◽

2019 ◽

Vol 4 (1) ◽

pp. 01-04

Author(s):

Heller Richard ◽

Guilan Shi

Keyword(s):

Computer Technology ◽

Early Stage ◽

Skin Surface ◽

Mortality Rates ◽

Skin Lesions ◽

The Body ◽

Non Invasive ◽

Pigmented Skin Lesions ◽

Early Tumor ◽

Melanoma Screening

Melanoma mortality rates are the highest amongst skin cancer patients. The 5-year survival rate is 92% if melanoma is diagnosed at an early stage and is localized. If melanoma has spread to other parts of the body, survival is only 13%. Therefore, it is particularly important to develop approaches that can enhance early and accurate diagnosis of melanoma. Dermatoscopy has been widely used to identify early tumor growth. With the addition of computer technology, digital dermatoscopy systems are capable of accurately assessing skin surface features modeled along the ABCD criteria and have been used for clinical assessment of pigmented skin lesions. This paper introduces a non-invasive computerized dermoscopy system that considers lesions on the skin for `diagnosis.

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Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals

Scientific Reports ◽

10.1038/s41598-021-95082-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Leyla A. Erozenci ◽

Sander R. Piersma ◽

Thang V. Pham ◽

Irene V. Bijnsdorp ◽

Connie R. Jimenez

Keyword(s):

Expression Patterns ◽

Interaction Network ◽

Extracellular Vesicle ◽

Protein Signaling ◽

Protein Patterns ◽

Specific Expression ◽

Non Invasive ◽

Data Independent Acquisition ◽

Time Period ◽

Using Data

AbstractThe protein content of urinary extracellular vesicles (EVs) is considered to be an attractive non-invasive biomarker source. However, little is known about the consistency and variability of urinary EV proteins within and between individuals over a longer time-period. Here, we evaluated the stability of the urinary EV proteomes of 8 healthy individuals at 9 timepoints over 6 months using data-independent-acquisition mass spectrometry. The 1802 identified proteins had a high correlation amongst all samples, with 40% of the proteome detected in every sample and 90% detected in more than 1 individual at all timepoints. Unsupervised analysis of top 10% most variable proteins yielded person-specific profiles. The core EV-protein-interaction network of 516 proteins detected in all measured samples revealed sub-clusters involved in the biological processes of G-protein signaling, cytoskeletal transport, cellular energy metabolism and immunity. Furthermore, gender-specific expression patterns were detected in the urinary EV proteome. Our findings indicate that the urinary EV proteome is stable in longitudinal samples of healthy subjects over a prolonged time-period, further underscoring its potential for reliable non-invasive diagnostic/prognostic biomarkers.

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Welfare Health and Productivity in Commercial Pig Herds

Animals ◽

10.3390/ani11041176 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1176

Author(s):

Przemysław Racewicz ◽

Agnieszka Ludwiczak ◽

Ewa Skrzypczak ◽

Joanna Składanowska-Baryza ◽

Hanna Biesiada ◽

...

Keyword(s):

Radio Frequency Identification ◽

Vital Signs ◽

Visual Assessment ◽

Early Warning Systems ◽

Warning Systems ◽

Herd Management ◽

Pork Production ◽

Antimicrobial Substances ◽

Non Invasive ◽

Frequency Identification

In recent years, there have been very dynamic changes in both pork production and pig breeding technology around the world. The general trend of increasing the efficiency of pig production, with reduced employment, requires optimisation and a comprehensive approach to herd management. One of the most important elements on the way to achieving this goal is to maintain animal welfare and health. The health of the pigs on the farm is also a key aspect in production economics. The need to maintain a high health status of pig herds by eliminating the frequency of different disease units and reducing the need for antimicrobial substances is part of a broadly understood high potential herd management strategy. Thanks to the use of sensors (cameras, microphones, accelerometers, or radio-frequency identification transponders), the images, sounds, movements, and vital signs of animals are combined through algorithms and analysed for non-invasive monitoring of animals, which allows for early detection of diseases, improves their welfare, and increases the productivity of breeding. Automated, innovative early warning systems based on continuous monitoring of specific physiological (e.g., body temperature) and behavioural parameters can provide an alternative to direct diagnosis and visual assessment by the veterinarian or the herd keeper.

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The Use of Micro- and Nanocarriers for Resveratrol Delivery into and across the Skin in Different Skin Diseases—A Literature Review

Pharmaceutics ◽

10.3390/pharmaceutics13040451 ◽

2021 ◽

Vol 13 (4) ◽

pp. 451

Author(s):

Beata Szulc-Musioł ◽

Beata Sarecka-Hujar

Keyword(s):

Transdermal Delivery ◽

Bacterial Infections ◽

Skin Diseases ◽

Skin Surface ◽

Non Invasive ◽

Skin Abnormalities ◽

Low Solubility ◽

Active Substances ◽

Poor Stability

In recent years, polyphenols have been extensively studied due to their antioxidant, anticancer, and anti-inflammatory properties. It has been shown that anthocyanins, flavonols, and flavan-3-ols play an important role in the prevention of bacterial infections, as well as vascular or skin diseases. Particularly, resveratrol, as a multi-potent agent, may prevent or mitigate the effects of oxidative stress. As the largest organ of the human body, skin is an extremely desirable target for the possible delivery of active substances. The transdermal route of administration of active compounds shows many advantages, including avoidance of gastrointestinal irritation and the first-pass effect. Moreover, it is non-invasive and can be self-administered. However, this delivery is limited, mainly due to the need to overpassing the stratum corneum, the possible decomposition of the substances in contact with the skin surface or in the deeper layers thereof. In addition, using resveratrol for topical and transdermal delivery faces the problems of its low solubility and poor stability. To overcome this, novel systems of delivery are being developed for the effective transport of resveratrol across the skin. Carriers in the micro and nano size were demonstrated to be more efficient for safe and faster topical and transdermal delivery of active substances. The present review aimed to discuss the role of resveratrol in the treatment of skin abnormalities with a special emphasis on technologies enhancing transdermal delivery of resveratrol.

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Correlation of Lesional Skin Corneometry Values with Serum E-Selectin Levels and Disease Severity in Patients Affected by Plaque-type Psoriasis: Recovery after Effective Therapy

The Journal of Dermatology ◽

10.1111/j.1346-8138.1995.tb03427.x ◽

1995 ◽

Vol 22 (7) ◽

pp. 475-479 ◽

Cited By ~ 7

Author(s):

Massimo Carducci ◽

Anna Mussi ◽

Claudio Bonifati ◽

Roberta Tomaselli ◽

Maria Teresa Onorati ◽

...

Keyword(s):

Disease Severity ◽

Effective Therapy ◽

Lesional Skin ◽

Plaque Type

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MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results

International Journal of Molecular Sciences ◽

10.3390/ijms22147241 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7241

Author(s):

Piotr K. Krajewski ◽

Weronika Szukała ◽

Agata Lichawska-Cieślar ◽

Łukasz Matusiak ◽

Jolanta Jura ◽

...

Keyword(s):

Western Blot ◽

Mrna Expression ◽

Hidradenitis Suppurativa ◽

Skin Lesions ◽

Healthy Controls ◽

Lesional Skin ◽

Chemotactic Protein ◽

Healthy Control ◽

Mrna And Protein Expression ◽

Skin Biopsies

The pathogenesis of hidradenitis suppurativa (HS) is yet to be fully understood. However, inflammation is a key element in the development of skin lesions. The aim of this study was to evaluate the expression of monocyte chemotactic protein-1-induced protein-1 (MCPIP1) in the skin of patients suffering from HS. Skin biopsies of 15 patients with HS and 15 healthy controls were obtained and processed for immunohistochemistry, western blot, and real time PCR. The highest mean MCPIP1 mRNA expression was found in the inflammatory lesional skin of HS patients. It was significantly higher than MCPIP1 mRNA expression in the biopsies from both healthy controls and non-lesional skin of HS patients. Western blot analysis indicated that expression of MCPIP1 was elevated within both lesional and non-lesional skin compared to the healthy control. The increased MCPIP1 mRNA and protein expression level in HS lesions may indicate its possible role in the disease pathogenesis.

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Development and Maintenance of Epidermal Stem Cells in Skin Adnexa

International Journal of Molecular Sciences ◽

10.3390/ijms21249736 ◽

2020 ◽

Vol 21 (24) ◽

pp. 9736

Author(s):

Jaroslav Mokry ◽

Rishikaysh Pisal

Keyword(s):

Stem Cells ◽

Expression Patterns ◽

Skin Surface ◽

Hair Cycle ◽

Epithelial Stem Cells ◽

Factors Associated ◽

Skin Cells ◽

Cytoplasmic Proteins ◽

Interfollicular Epidermis ◽

Skin Adnexa

The skin surface is modified by numerous appendages. These structures arise from epithelial stem cells (SCs) through the induction of epidermal placodes as a result of local signalling interplay with mesenchymal cells based on the Wnt–(Dkk4)–Eda–Shh cascade. Slight modifications of the cascade, with the participation of antagonistic signalling, decide whether multipotent epidermal SCs develop in interfollicular epidermis, scales, hair/feather follicles, nails or skin glands. This review describes the roles of epidermal SCs in the development of skin adnexa and interfollicular epidermis, as well as their maintenance. Each skin structure arises from distinct pools of epidermal SCs that are harboured in specific but different niches that control SC behaviour. Such relationships explain differences in marker and gene expression patterns between particular SC subsets. The activity of well-compartmentalized epidermal SCs is orchestrated with that of other skin cells not only along the hair cycle but also in the course of skin regeneration following injury. This review highlights several membrane markers, cytoplasmic proteins and transcription factors associated with epidermal SCs.

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