scholarly journals “Porcine Digestible Peptides (Pdp) in Weanling Diets Improves Gut Barrier Function, Immune Response and Nutrient Transport in Nursery Pigs” 

2020 ◽  
Author(s):  
Francesc González-Solé ◽  
Lourdes Criado-Mesas ◽  
Carmen Villodre ◽  
Wellington C. García ◽  
Mercè Farré ◽  
...  
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Barrier Function ◽  
Average Daily Gain ◽  
Nutrient Transport ◽  
Oxidation Products ◽  
Partial Substitution ◽  
Intestinal Function ◽  
Commercial Diet ◽  
Gut Barrier Function

Abstract Background: Porcine digestive peptides (PDP) have proven to be a valid substitute for other high-quality dietary protein sources for piglets, like spray-dried plasma (SDP), but knowledge about their influence on intestinal function is still scarce. This study was conducted to investigate the effects of PDP, SDP or a combination of both on growth performance and intestinal function of weaned pigs. Results: A total of 180 piglets (Trial 1) and 198 piglets (Trial 2) were used to evaluate the partial substitution of soybean ingredients with 2% SDP or 2% PDP (Trial 1), and with 3% SDP or the combination of 1% SDP and 2% PDP (SDP-PDP; Trial 2) during the pre-starter period (d0-14). A common commercial diet was offered up to day 35 in Trial 2. The effects on intestinal function were evaluated by quantifyingthe gene expression (56 genes in an Open Array Real-Time PCRPlatform) in jejunum and ileum samples obtained from piglets on d 14 after weaning (Trial 2).Piglets fed SDP, PDP and SDP-PDP had a higher body weight (BW), average daily gain (ADG) and feed efficiency (G:F) than the soybean controlon day 14 (P< 0.05), but no differences were observed among groups at the end of starter phase (Trial 2). Ten genes in jejunum and five in ileum samples were differentially expressed among treatments (P< 0.05). The highest expression was found in the SDP-PDP group. These genes are involved in the barrier function of intestinal cells,the immune response, mucosal nutrient transport, digestion and metabolization of oxidation products. Conclusion: The combination of SDP and PDP upregulated genes related to the intestinal functionwithout affecting growth performance. The underlying mechanisms that produced these effects are still unknown; however, protein hydrolysates might contain bioactive peptides that could be the cause.

scholarly journals Porcine Digestible Peptides (PDP) in Weanling Diets Regulates the Expression of Genes Involved in Gut Barrier Function, Immune Response and Nutrient Transport in Nursery Pigs

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2368
Author(s):  
Francesc González-Solé ◽  
Lourdes Criado-Mesas ◽  
Carmen Villodre ◽  
Wellington C. García ◽  
Mercè Farré ◽  
...  
Keyword(s):  
Gene Expression ◽  
Average Daily Gain ◽  
Partial Substitution ◽  
Intestinal Function ◽  
Nursery Pigs ◽  
Gut Barrier Function ◽  
Expression Of Genes ◽  
Qpcr Platform ◽  
Underlying Mechanisms ◽  
Daily Gain

This study was conducted to investigate the effects of dietary supplementation of porcine digestible peptides (PDP), spray-dried plasma (SDP), or a combination of both, on growth performance and the expression of genes related to intestinal function of weaned pigs. A total of 180 piglets (trial 1) and 198 piglets (trial 2) were used to evaluate the partial substitution of soybean ingredients with 2% SDP or 2% PDP (trial 1), and with 3% SDP or the combination of 1% SDP and 2% PDP (SDP-PDP; trial 2) during the pre-starter period (0–14 days). The gene expression of 56 genes was quantified in a qPCR platform in jejunum and ileum samples obtained from piglets 14 d after weaning (trial 2). Piglets fed SDP, PDP and SDP-PDP had a higher body weight (BW), average daily gain (ADG) and feed efficiency (G:F) than the soybean control on day 14 (p < 0.05). In addition, the combination of SDP and PDP upregulated ten genes in jejunum samples (p < 0.05) related to intestinal function. More research is needed to confirm that gene expression upregulation by PDP in combination with SDP has an impact on intestinal function and to elucidate its underlying mechanisms.

scholarly journals Effects of Rocket Seed Oil, Wheat Germ Oil, and Their Mixture on Growth Performance, Feed Utilization, Digestibility, Redox Status, and Meat Fatty Acid Profile of Growing Rabbits

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 662
Author(s):  
Sabrin Abdelrahman Morshedy ◽  
Ahmed M. Abdelmodather ◽  
Mohamed M. Basyony ◽  
Soliman A. Zahran ◽  
Mohamed A. Hassan
Keyword(s):  
Immune Response ◽  
Fatty Acid ◽  
Growth Performance ◽  
Wheat Germ ◽  
Seed Oil ◽  
Average Daily Gain ◽  
Feed Utilization ◽  
Nutrient Digestibility ◽  
Control Group ◽  
Wheat Germ Oil

Vegetable oils are a source of natural antioxidants, including tocopherols, sterols, phenolic compounds, coenzymes, and polyunsaturated fatty acids that provide nutritional value, organoleptic properties, and significantly delay or prevent lipid oxidation. Eighty-four V-line rabbits at 5 weeks of age with an initial body weight (BW) of 535.60 ± 13.48 g were assigned randomly to four experimental groups (seven replicates in each group with three rabbits each). The first group served as a control and received 0.3 mL/kg BW of distilled water (CON), while the second and third groups received 0.3 mL/kg BW of rocket seed oil (RSO) and wheat germ oil (WGO), respectively. The fourth group received a mixture of oils consisting of 0.15 mL of RSO and 0.15 mL of WGO/kg BW (MOs). The experiment lasted 7 weeks. The study investigated the effects of RSO, WGO, and their mixture on growth performance, feed utilization, antioxidant status, and immune response of growing rabbits. The results indicated that the rabbits that were administered orally with RSO and WGO or their mixture had higher (p ≤ 0.05) final BW, weight gain, and average daily gain when compared to the control group. In addition, the feed conversion ratio improved significantly with RSO, WGO, and MOs treatments. Different oil treatments improved nutrient digestibility, nutritive value, and nitrogen balance. Moreover, the rabbits that received RSO, WGO, and their mixture had an improvement the meat fatty acid composition compared to the control rabbits. Oral administration of RSO, WGO, and their mixture significantly improved serum protein fractions, decreased blood urea nitrogen, and had a positive effect on serum total lipids, HDL-c, and LDL-c. Furthermore, the treatments of RSO, WGO, and MOs had a significant improvement in the antioxidative status and immune response.

scholarly journals Transversal gene expression panel to evaluate intestinal health in broiler chickens in different challenging conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
L. Criado-Mesas ◽  
N. Abdelli ◽  
A. Noce ◽  
M. Farré ◽  
J. F. Pérez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Barrier Function ◽  
Nutrient Transport ◽  
Gene Expression Pattern ◽  
Vaccine Dose ◽  
Special Focus ◽  
Intestinal Health ◽  
Barrier Failure ◽  
Expression Of Genes

AbstractThere is a high interest on gut health in poultry with special focus on consequences of the intestinal diseases, such as coccidiosis and C. perfringens-induced necrotic enteritis (NE). We developed a custom gene expression panel, which could provide a snapshot of gene expression variation under challenging conditions. Ileum gene expression studies were performed through high throughput reverse transcription quantitative real-time polymerase chain reaction. A deep review on the bibliography was done and genes related to intestinal health were selected for barrier function, immune response, oxidation, digestive hormones, nutrient transport, and metabolism. The panel was firstly tested by using a nutritional/Clostridium perfringens model of intestinal barrier failure (induced using commercial reused litter and wheat-based diets without exogenous supplementation of enzymes) and the consistency of results was evaluated by another experiment under a coccidiosis challenge (orally gavaged with a commercial coccidiosis vaccine, 90× vaccine dose). Growth traits and intestinal morphological analysis were performed to check the gut barrier failure occurrence. Results of ileum gene expression showed a higher expression in genes involved in barrier function and nutrient transport in chickens raised in healthy conditions, while genes involved in immune response presented higher expression in C.perfringens-challenged birds. On the other hand, the Eimeria challenge also altered the expression of genes related to barrier function and metabolism, and increased the expression of genes related to immune response and oxidative stress. The panel developed in the current study gives us an overview of genes and pathways involved in broiler response to pathogen challenge. It also allows us to deep into the study of differences in gene expression pattern and magnitude of responses under either a coccidial vaccine or a NE.

Combined administration of bacteriocin- -producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits

2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  
Keyword(s):  
Growth Performance ◽  
Enterococcus Faecium ◽  
Average Daily Gain ◽  
Probiotic Strain ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Feed Conversion ◽  
Eleutherococcus Senticosus ◽  
Commercial Diet ◽  
And Control

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.

scholarly journals Effects of Fermented Feed on the Growth Performance, Intestinal Function and Microbiota of Piglets Weaned at Different Age

2021 ◽  
Author(s):  
Shuai Liu ◽  
Yunxia Xiong ◽  
Jingping Chen ◽  
Hao Xiao ◽  
Qiwen Wu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Barrier Function ◽  
Control Diet ◽  
Intestinal Barrier ◽  
Dietary Supplementation ◽  
Short Chain Fatty Acids ◽  
Intestinal Barrier Function ◽  
Intestinal Function ◽  
Feed Conversion ◽  
Fermented Feed

Abstract BACKGROUND: The beneficial function of fermented feed in livestock industry has been widely investigated. However, little is known about the effects of fermented feed on different weaned-day piglets. This study aimed to investigate the effects of fermented diet on the growth performance, intestinal function and microbiota of piglets weaned at age of 21 days and 28 days.RESULTS: The results found that weaning on d 21 significantly increased (P < 0.05) ADG, and ADFI (calculated based on wet weight and dry matter), while reduced (P < 0.05) F: G, the activities of trypsin and lipase of jejunum and villus height of ileum, compared with 28-d weaning. The protein levels of Occludin, Claudin-1, ZO-1 of ileum in the groups weaning on d 21 were less (P < 0.05) than the groups weaning on d 28. Moreover, dietary supplementation with fermented diet upregulated (P < 0.05) Occludin, Claudin-1, ZO-1 proteins of ileum, compared with the groups treated with control diet both weaning on d 21 and d 28. In addition, dietary supplementation with fermented diet decreased (P < 0.05) the relative abundance of Clostridia (class) and increased (P < 0.05) Bacteroidia (class) level of cecal microbiota, compared with the groups treated with control diet both weaning on d 21 and d 28. However, supplementation with fermented diet did not affect the concentrations of short-chain fatty acids in the cecum (P > 0.05).CONCLUSION: Therefore, our data suggest that feed digestibility is improved in piglets weaned at 21 days, but intestinal barrier function is weaker than in piglets weaned at 28 days. However, compared with feeding control diet, supplementation with fermented diet both improved feed conversion and intestinal barrier function of weaned piglets by modulating intestinal microbiota.

153 Impact of xylanase and an enzyme blend on gut barrier function and growth performance in nursery pigs

2017 ◽  
Vol 95 (suppl_2) ◽  
pp. 72-73
Author(s):  
Q. Y. Li ◽  
N. K. Gabler ◽  
C. Sparks ◽  
J. F. Patience
Keyword(s):  
Growth Performance ◽  
Barrier Function ◽  
Gut Barrier ◽  
Nursery Pigs ◽  
Gut Barrier Function

scholarly journals Effects of Dietary Taurine Supplementation to Gilts during Late Gestation and Lactation on Offspring Growth and Oxidative Stress

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 220 ◽  
Author(s):  
Mengmeng Xu ◽  
Long Che ◽  
Kaiguo Gao ◽  
Li Wang ◽  
Xuefen Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Expression ◽  
Growth Performance ◽  
Barrier Function ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Late Gestation ◽  
Villus Height ◽  
And Oxidative Stress

Birth is one of the most important events of animal production agriculture, as newborns are abruptly forced to adapt to environmental and nutritional disruptions that can lead to oxidative damage and delay in growth. Taurine (Tau) is an important regulator of oxidative stress and possesses growth-enhancing properties. In the present study, we investigated the effects of dietary Tau supplementation in gilts during late gestation and lactation on the growth performance of piglets by assessing intestinal morphology and barrier function, and oxidative stress status. Sixteen gilts were randomly allocated to the Con (basal diet) and Tau (basal diet with 1% Tau) groups from 75 d of gestation to weaning. Maternal dietary Tau supplementation significantly increased weaning weight and average daily gain weight in piglets. Piglets in the Tau group had higher villus height and villus height-to-crypt depth ratio (VCR), ZO-1 protein expression, and secretory immunoglobulin A (sIgA) content in the jejunum. Meanwhile, Tau bebeficial affected the milk quality of gilts, as indicated by decreased malondialdehyde (MDA) concentration and increased total superoxide dismutase (T-SOD), total antioxidative capability (T-AOC), glutathione peroxidase (GPx), and catalase (CAT) activity. Furthermore, Tau supplementation increased T-SOD activity in plasma and SOD2 protein expression in the jejunum in the piglets. In conclusion, this study provides evidence that dietary Tau supplementation to gilts improves growth performance in piglets, owing to improved intestinal morphology and barrier function, as well as inhibition of oxidative stress.

scholarly journals PSVI-2 Identification of correlations between aldehyde profiles of thermally oxidized soybean oils and growth performance of broilers

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 204-205
Author(s):  
Jieyao Yuan ◽  
Brian Kerr ◽  
Chi Chen
Keyword(s):  
Soybean Oil ◽  
Growth Performance ◽  
Feed Intake ◽  
Average Daily Gain ◽  
Oxidation Products ◽  
Soybean Oils ◽  
Oxidized Oil ◽  
Lipid Oxidation Products ◽  
Pls Analysis ◽  
Daily Gain

Abstract Feeding oxidized oils can cause adverse effects on the growth performance of broilers, which have largely attributed to the presence of lipid oxidation products (LOP) in oxidized oils. This study was conducted to identify the potential correlations between growth performance and aldehyde profiles of differently-prepared thermally oxidized soybean oils. Seventy-seven broilers were randomly assigned to one of seven diets containing 7.5% fresh soybean oil or thermally oxidized soybean oil (OSO), including 1) 22.5°C, 0h; 2) 45°C, 336h; 3) 67.5°C, 168h; 4) 90°C, 84h; 5) 135°C, 42h; 6) 180°C, 21h; 7) 225°C, 10.5h, respectively. Broilers were provided ad libitum access to experimental diets for 28 days. Broilers body weight (BW) and feed intake were recorded weekly to calculate the average daily gain (ADG), average daily feed intake (ADFI), and gain to feed (GF) ratio. The aldehydes in these oils were determined by the liquid chromatography-mass spectrometry (LC-MS) analysis. The correlations between individual aldehydes and ADG, ADFI, or GF were examined by partial least squares (PLS) analysis. Feeding 135°C, 42h oxidized oil significantly decreased the ADG, ADFI, and GF value compared with other groups (p < 0.001). Among 17 aldehydes identified in thermally oxidized soybean oils, C9-C11 unsaturated alkenals, including 2-decenal, 2-undecenal, 2,4-nonadienal, 4-hydroxy-2-nonenal, 2,4-decadienal, and 2,4-undecadienal, had better inverse correlations with growth performance than C5-C8 saturated alkanals in broilers, suggesting different toxic effects from these aldehydes.

scholarly journals Effects of species-specific probiotic addition to milk replacer on calf health and performance during the first month of life

2014 ◽  
Vol 14 (1) ◽  
pp. 101-115 ◽  
Author(s):  
Alessandro Agazzi ◽  
Erica Tirloni ◽  
Simone Stella ◽  
Serena Maroccolo ◽  
Barbara Ripamonti ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Bacillus Coagulans ◽  
Basal Diet ◽  
Milk Replacer ◽  
Blood Eosinophil ◽  
Feed Conversion ◽  
Species Specific

AbstractThe aim of this study was to evaluate the effects of the administration of a species-specific probiotic (Lactobacillus animalis SB310, Lactobacillus paracasei subsp. paracasei SB137 and Bacillus coagulans SB117 in a 30:35:35 ratio, respectively; 1.8 × 1010 CFU/g of powder) on gut microbial balance, immune response and growth performance of Holstein female calves during the first month of life. Twenty-two calves were divided into two experimental groups from 2 to 28 days of life: control (C), fed with milk replacer and concentrate as a basal diet, and treatment (T), fed C diet plus 1 g/calf/day of probiotic powder for the first month of age. Faecal and blood samples were individually collected and analysed weekly. Individual faecal score was recorded daily and general health score was calculated at the end of the trial. Cell-mediated immune response was evaluated by skin test at 7 and 28 days of life. Milk replacer and concentrate intake were recorded daily, while body weight and biometrical parameters were recorded at 2, 8, 14, 21 and 28 days of life, thus average daily gain and feed conversion rate were calculated. During the first week of treatment, lower blood eosinophil percentage (0.05% vs. 0.22%; P≤0.01) was found in T group, while basophils were higher in T than C group at the end of the trial (0.21% vs. 0.16%; P≤0.05). Higher faecal lactic acid bacteria (LAB)/E. coli ratio on day 28 of life (3.73 log CFU/g vs. 2.02 log CFU/g; P≤0.05) and lower incidence of diarrhoea were found in the treated group (63.30% vs. 70.71%; P=0.05). Body weight (48.92 kg vs. 46.92 kg; P≤0.05), total concentrate intake (14.77 kg vs. 12.56 kg on dry matter basis; P≤0.05), and heart girth (81.16 cm vs. 78.49 cm; P≤0.05) were significantly higher in T group. The administration of the probiotic during the first month of life improved gut microbiota and increased the growth performance and some biometric parameters of calves.

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