scholarly journals The Biological-promoting Function of Dietary Nano-selenium, Seaweed Gracilaria Lemaneiformis and Nano-selenium-enriched Gracilaria Lemaneiformis on Growth Performance, Antioxidant and Immunity Systems and Meat Quality of Grouper (Epinephelus Coioides)

Author(s):  
Changpeng Ye ◽  
Jiaojiao Yu ◽  
Yuying Huang ◽  
Chaowen Zhang ◽  
Min Wu ◽  
...  

Abstract Background: Nano-Se-enriched Gracilaria lemaneiformis (G. lemaneiformis) as feed ingredient on aquatic animals remained largely unknown. Feeding experiment was conducted for a total of 45 days to research the biological impacts of nano-selenium (Nano-Se) (group N), G. lemaneiformis (group G) and Nano-Se-enriched G. lemaneiformis (group NG) on growth, selenium concentration, oxidation, immunity and nutrition of Epinephelus coioides.Results: At each tested time point, the weight gain rate, specific growth rate and feed conversion rate of group N, group G and group NG were significantly increased in contrast to those of the control group (group C). A remarkable increase of the hepatosomatic index (HSI) and viscerosomatic index (VSI) was observed in group N and group NG. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of fish in group N, group G and group NG were significantly enhanced relative to those in group C. The immune system was also activated by showing an elevated expression level of various immunological genes including Immunoglobulin M, tumor necrosis factor-α and Interleukin -8. The muscle Se content in group N and group NG was 31- and 40.5-fold that of the control group (group C), respectively. The liver Se value of group N and group NG was 1.06 and 2.50 times higher than that of group C, respectively. The lipid and moisture values of group N and group NG are increased and reduced, respectively, in comparison to that of group C. The diet supplementation of Nano-Se-enriched G. lemaneiformis led to significant increase of the protein content, delicious amino acids along with total amino acids content in grouper. Conclusions: Our results demonstrate a great application potential of Nano-Se-enriched G. lemaneiformis as dietary additive in grouper.

2011 ◽  
Vol 51 (No. 10) ◽  
pp. 449-457 ◽  
Author(s):  
S. Ševčíková ◽  
M. Skřivan ◽  
G. Dlouhá ◽  
M. Koucký

The effect of dietary supplementation of selenium in an organic form on performance, carcass traits and selenium content in tissues of broiler cockerels Ross 308 was studied. The soya-wheat-maize diet contained 50 mg vitamin E/kg. The experiment was conducted on 810 straight-run broiler cockerels randomly divided into 3 groups: group I – control, without selenium supplement; experimental group: II – 0.3 mg Se/kg, Se-enriched yeast was applied as a Se source; III – 0.3 mg Se/kg, Se-enriched alga Chlorella as a Se source. The broiler chickens were slaughtered at 42 days of age. In performance traits higher (P ≤ 0.05) live weight of broiler chickens was recorded in the experimental groups (II – 2 430.6 g and III – 2 425.2 g). There were no significant differences between the groups in feed conversion and mortality. Se-enriched alga had the best feed conversion, and selenium supplementation slightly increased mortality in both experimental groups. No significant differences between the groups were found out in carcass traits and dressing percentage. The content of selenium in breast and thigh muscle, feathers and excrements increased (P ≤ 0.05) in both experimental groups compared to the control group. Higher values in breast and thigh muscle and in feathers were measured in the group supplemented with selenium from Se-enriched yeast, also in comparison with the group supplemented with selenium from Se-enriched alga Chlorella. The broiler chickens receiving Chlorella had a higher (P ≤ 0.05) selenium content in excrements compared to the group with Se-enriched yeast. The selenium concentration in liver was higher (P ≤ 0.05) in both experimental groups compared to the control. The supplement of selenium from Se-yeast and Chlorella in the diet for broiler chickens increased the microelement concentration in muscle.    


Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3046
Author(s):  
Yinyin Chen ◽  
Xiaoxiao Gong ◽  
Tianyu Yang ◽  
Maocheng Jiang ◽  
Lin Wang ◽  
...  

Sixty castrated male Haimen white growing goats with an initial age of 100 days old and similar body weight (16 ± 1.5 kg) were selected and randomly allocated into five groups with three replicates in each group with four goats in each pen (5 m × 3.2 m). Goats in the control group (CG) were fed a basal pelleted total mixed ration supplemented with 30% alfalfa hay pellet, while experimental treatments (G6, G12, G18, or G24) were supplemented with four levels (6%, 12%, 18%, or 24%) of GBLR replacing alfalfa hay pellet in the diet, separately. Results showed that (1) the final body weight, average daily gain, and average feed intake of G18 was significantly higher (p < 0.05) than CG; in contrast, the feed conversion ratio of G18 was significantly lower than CG and G12 (p < 0.05); the feed cost per head per day of CG was significantly higher (p < 0.05) than that of G18 and G24, and exhibited obvious linearly decrease (p = 0.04) with increasing GBLR supplementation; and apparent total-tract digestibility of DM and NDF in GBLR treatments were significantly higher (p < 0.05) than CG; (2) alanine transaminase (ALT) concentration in the G18 group was significantly lower (p < 0.05) than those in the control group; aspartate transaminase (AST) concentration in the G24 was significantly lower (p < 0.05) than those in the control group, and an increase in dietary level of GBLR tended to result in a linear decrease (p = 0.09) in the concentration of serum AST; (3) the concentration of malondialdehyde (MDA) demonstrated a tendency to decrease (p = 0.06) linearly with increasing GBLR supplementation; however, glutathione peroxidase (GSH-PX) activity in G12 was significantly higher (p < 0.05) than CG, G6, and G24; in addition, superoxide dismutase (SOD) activity in G18 was significantly higher (p < 0.05) than CG and G6; concentration of immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA) were not affected by GBLR, but increasing dietary GBLR showed a tendency (p = 0.08) to linearly increase the IgG concentration; the content of interleukin 4 (IL4) was significantly higher (p < 0.05) in G12, G18, and G24 than that in CG and G6; (4) There were similar NH3-N, pH, TVFA, and butyrate for goats fed different levels of GBLR supplementation; the C2 (p = 0.07) and acetate: propionate (p = 0.06) demonstrated a tendency to increase linearly with increasing level of GBLR supplementation, separately; however, it was observed that concentration of propionate showed a tendency to decrease (p = 0.08) linearly in response to GBLR supplementation; and (5) Increasing dietary GBLR tended to linearly enhance the lightness (L*) (p = 0.07) and yellowness (b*) (p = 0.09) values of longissimus dorsi muscles; the redness (a*) value in G18 was significantly higher than that in CG (p < 0.05).


2020 ◽  
Author(s):  
Hang Zhao ◽  
Saikun Pan

Abstract Background: The present study aimed to explore the effects of dietary chitosan oligosaccharides (COs) on the growth performance and immunity of broiler chickens.Methods: Four test diets were supplemented with different doses of COs (0, 50, 100 and 150 mg/kg) and formulated. Triplicate groups of broiler chickens were fed with one of the diets ad libitum for 6 weeks. Results: After the end of the feeding trials, oral CO administration improved average daily gain; the activities of digestive amylase, lipase and protease; the activities of serum superoxide dismutase and glutathione peroxidase; serum tumor necrosis factor-α, interleukin (IL)-1, IL-2, IgG, IgM and IgA levels; and reduced feed conversion ratio and malondialdehyde level compared with those of the control group. However, a high dose of CO (150 g/kg) did not further increase its efficiency compared with the moderate dose of CO (100 g/kg). Conclusions: Oral CO administration improved the growth performance and immunity of juvenile broiler chickens and could be used as a feed supplement.


2020 ◽  
Vol 20 (2) ◽  
pp. 133-142
Author(s):  
Palash Bala ◽  
Ghulam Mustafa ◽  
Shankar Chandra Mandal

Pangas catfish, Pangasius hypophthalmus is one of the most widely cultured fish species in Asia. The growth and flesh quality of fish depend largely on the quality and composition of feed. Inclusion of exogenous enzyme can improve the palatbility and digestibility of feed. However, there is a lack of research on the use of exogenous enzymes in fish diet for improved growth and nutritional quality of fish. Thus, in the current study, the effects of enzyme inclusion in fish diet were evaluated using pangas catfish as an experimental animal. The study was conducted in a laboratory condition with a flow-through system where 12 plastic tanks with 750 L water capacity were used where the tanks were grouped randomly. Each tank was stocked with 15 fingerlings of pangas catfish. Three different concentrations (3, 4 and 5% of the total feed) of Zymex, a commercially available ulti-enzyme were used for treatment groups along with a control group with no enzyme supplementation. The length-weight relationship of the experimental fish using the initial and final data was established where a significantly positive relationship was found (P<0.05). Growth performance including condition factor, specific growth rate and feed conversion ratio was significantly improved by 3% enzyme supplementation (P<0.05). Average daily gain did not vary significantly between treatments. Three percent enzyme supplementation in the diet significantly increased crude protein and lipid contents and reduced moisture content (P<0.05). Significantly higher ash content was found in fish fed both 3 and 5% enzyme contained diet (P<0.05). In case of body indices, 5% enzyme supplementation provided significantly higher viscerosomatic index (P<0.05) while 3% provided significantly higher flesh yield (P<0.05). However, hepatosomatic index did not vary significantly between treatments. From the findings it is concluded that enzyme supplementation at 3% of the diet can improve the growth performance and nutritional quality of pangas catfish.


2018 ◽  
Vol 17 (03) ◽  
pp. 29-34
Author(s):  
Mai C. Duong

The experiment was conducted to determine the effects of four amino acids (lysine, methionine, threonine and tryptophan) supplementation on growth performances and serum antibody titers to Gumboro disease (IBD, infectious bursal disease) in broiler chickens. Chicks were randomly assigned to 5 dietary groups (5 chicks/group as 12 replicates of 5 chicks) in a complete randomized experimental design. Group I served as control group, was fed a diet without any supplementation. Groups II and III were supplemented 10% of four amino acids (lysine, methionine, threonine and tryptophan) of the recommended requirements for 45 and 42 days, respectively. Meanwhile, Groups IV and V were supplemented 20% of four amino acids (lysine, methionine, threonine and tryptophan) of the recommended requirements for 45 and 42 days, respectively. The supplementation was started at 3 days of age in chicks. The chickens were vaccinated against Gumboro disease at day 12 and day 19. The antibody titer of the chickens in each group was assayed using IBD anti-body ELISA. Besides, the live body weight, average feed intake and feed conversion (FCR) were determined at 0, 21, 42 and 84 days of age. The results showed no significant difference between groups in performance parameters such as body weight and FCR. However, the present work showed highest dose of lysine, methionine, threonine and tryptophan supplementation for 42 days may be improved feed intake and immune response of chicken against Gumboro disease vaccination


2015 ◽  
Vol 7 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Oğuz Kose ◽  
Varol Canakcı ◽  
Cenk Fatih Canakcı ◽  
Abdulkadir Yıldırım ◽  
Eda Kermen ◽  
...  

Background and aims. The aim of this study was to evaluate the possible effects of obesity on the local (salivary) and systemic TNF-α and IL-6 levels in patients with chronic periodontitis (CP). Materials and methods. This study included 88 subjects assigned to four groups of 22 subjects each, as follows: group O+P+ (patients with obesity and CP), group O-P+ (patients with normal weight and CP), group O+P- (periodontally healthy patients with obesity), and the control group, group O-P- (periodontally healthy patients with normal weight). Serum and salivary samples were obtained a week before the recording of clinical periodontal parameters. Local and systemic TNF-α and IL-6 levels were determined biochemically. Results. In serum and saliva, both TNF-α and IL-6 levels were the lowest in O-P- group (P < 0.05). The highest TNF-α and IL-6 levels were observed in O+P+ group, while only IL-6 levels were statistically significant (P < 0.05). Conclusion. Obesity upregulated the salivary and serum levels of TNF-α and IL-6. In patients with periodontitis, who were also obese, the serum and saliva levels of IL-6 were significantly high. Obesity might play a destructive and provocative role in the pathogenesis of periodontitis by negatively affecting IL-6 levels.


2020 ◽  
Vol 01 ◽  
Author(s):  
Hong-Ling Yang ◽  
Xi Hu ◽  
Ji-Dan Ye ◽  
Vijayaram Seerengaraj ◽  
Wei Yang ◽  
...  

Background:: Probiotic cellular components could be an interesting alternative to live probiotics, which could potentially cause safety problems in open aquatic environments. Objective:: The cell wall (CW), peptidoglycan (PG) and lipoteichoic (LTA) were extracted from probiotic strain of Bacillus pumilus SE5, and these biomolecules were used to develop the possible application in fish aquaculture. Methods:: Grouper (Epinephelus coioides) juveniles were fed with either a basal control diet or the basal diet supplemented with CW, PG and LTA respectively for 60 days, and the growth performance, digestive enzymes activities, serum immune responses and immune genes expression in head kidney were determined. Results:: Dietary supplement PG and LTA significantly improved final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER), while significantly decreased feed conversion ratio (FCR) was noticed in all the treatments compare with the control group. Dietary supplements of CW, PG and LTA enhanced the activity of trypsin, lipase and amylase activities in liver. Serum complement C3 and IgM levels as well as AKP, ACP and SOD activities elevated significantly in fish fed the PG and LTA containing diets. Furthermore, PG and LTA containing diets significantly up-regulated expression of TLR2, NOD2, IL-8, IgM and three antibacterial peptides genes (epinecidin-1, hepcidin- 1 and β-defensin) in the head kidney. Conclusion:: PG and LTA originated from probiotic B. pumilus SE5 could effectively enhance the growth performance, feed utilization, digestive ability and boost innate and adaptive immune system of E. coioides.


2020 ◽  
Vol 17 (2) ◽  
Author(s):  
M. S. U. Akon ◽  
M.R. Amin ◽  
M. T. Hussan ◽  
R. Akhter ◽  
M. L. Mollah

Background: Recently, the use of antibiotic growth promoters has been banned in many countries due to cross-resistance among pathogens and residues in tissues, which led to search for the alternative feed additives in animal production. The aim of this study was to investigate the effects of “Kalo jeera” (Nigella sativa) powder and amino acids supplementation on the growth of broiler chicks and their blood parameters. Methods: A total of 100 day-old (Cobb® 500) chicks were randomly divided into four groups such as T0, T1, T2, T3, and each group contained 25 chicks. The group T0 was used as control and groups T1, T2 and T3 were treated group. Chicks of control group were fed with only basal diet and no supplements were supplied, and chicks of treatment group including T1, T2 and T3 were supplemented with amino acids, Nigella sativa powder and combination of Nigella sativa and amino acids, respectively. Body weight was recorded on 7th, 14th, 21th, 28th and 35th day of age. Haematological tests were performed at 15th, 25th and 35th day of age. Results: The results showed that net weight gain and feed conversion ratio were improved in the treated groups than that of the control group. Among the treated groups, the significant level of improvement was achieved in the T3 group followed by T1 and then T2. Total erythrocyte count, packed cell volume and haemoglobin concentration were significant (p<0.01) in the treated groups as compared to control. Conclusions: The results suggest that better growth performance could be achieved in broilers supplemented with Nigella sativa and amino acids in combination and those can be used as growth promoters.


2016 ◽  
pp. 108-111
Author(s):  
T.F. Tatarchuk ◽  
◽  
D.G. German ◽  

The article presents the comparative analysis of the state of the cervix in women with endometrial polyps and micropolyps. Patients and methods. The study involved 130 patients aged 18-35 years: 70 patients with endometrial polyps (group I), 30 patients with micropolyps (group II) and 30 patients of the control group (group III). Results. According to the anamnesis of women in the I group were significantly more frequent diseases of the cervix, which corrected physical surgery methods, in particular cryodestruction. In group II, the representatives of these indicators were similar to healthy. Normal colposcopic picture met significantly less frequently in patients and I, and II group. The differences in the incidence of HPV high oncogenic risk in all groups were not statistically significant. Conclusion. Destructive methods used in the detection of any changes in the cervix are often overly aggressive, form scars and contributing to inflamaciones process. In the chain of events leading to the formation of PE, cervical pathology and its correction can take the basic place. Key words: endometrial polyp, micropolyps, chronic endometritis, uterine cervix, colposcopy.


2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.


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