scholarly journals Specific serodiagnosis of animal brucellosis based on a recombinant multiepitope protein antigen

2020 ◽  
Author(s):  
Dehui Yin ◽  
Qiongqiong Bai ◽  
Han Li ◽  
Jinpeng Zhang
Keyword(s):  
Diagnostic Accuracy ◽  
Bovine Serum ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Animal Husbandry ◽  
Protein Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Important Public Health ◽  
Personal Property

Abstract Background: Brucellosis is a vital zoonotic infectious disease that will cause important public health problems and endanger the development of animal husbandry in endemic areas, causing huge losses of personal property. Early diagnosis of sick animals is a crucial way to reduce the incidence of brucellosis.Results: In this study, we designed a recombinant multiepitope protein (rMEP) as a serum diagnostic antigen for brucellosis. An indirect enzyme-linked immunosorbent assay (ELISA) was used to assess the new rMEP, 159 goat and 153 bovine serum samples were measured, including brucellosis and non-brucellosis. To better observe the effectiveness of rMEP, we performed a receiver operating characteristic (ROC) analysis. The results of 159 goat serum evaluations showed that the area under the ROC curve(AUC) was 0.9976 and compared with SAT and RBPT, the positive and negative diagnostic accuracy of the ELISA was 98.92%(92/93) and 96.97%(64/66), respectively. 153 bovine serum samples evaluations showed the AUC was 0.9974 and compared with SAT and RBPT, the positive and negative diagnostic accuracy of the ELISA was 98.65%(73/74) and 96.20%(76/79), respectively.Conclusions: The results show that rMEP as a protein antigen can be used to diagnose brucellosis with highly accurate both in goat and bovine.

scholarly journals Specific Serodiagnosis of Animal Brucellosis Based on A Recombinant Multiepitope Protein Antigen

2021 ◽  
Author(s):  
Linlin Xu ◽  
Qiongqiong Bai ◽  
Jinpeng Zhang ◽  
Yang Jiao ◽  
Dehui Yin
Keyword(s):  
Roc Curve ◽  
Bovine Serum ◽  
Animal Husbandry ◽  
Diagnostic Value ◽  
Agglutination Test ◽  
Protein Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Roc Curve Analysis ◽  
Personal Property

Abstract Background: Brucellosis is a zoonotic infectious disease that causes substantial public health problems and endangers the development of animal husbandry in endemic areas, causing huge losses of personal property. Early diagnosis of sick animals is a crucial step in reducing the incidence of brucellosis.Objective: In this study, we designed a recombinant multiepitope protein (rMEP) as a serum diagnostic antigen for brucellosis and evaluated its diagnostic value in cattle and goats.Methods: An indirect enzyme-linked immunosorbent assay (iELISA) was used to assess the new rMEP, and 159 goat and 153 bovine serum samples were measured, including brucellosis and nonbrucellosis samples. To better observe the effectiveness of rMEP, we performed receiver operating characteristic (ROC) curve analysis.Results: Evaluation of the 159 goat serum samples showed that the area under the ROC curve (AUC) was 0.9976, and compared with serum tube agglutination test (SAT) and the Rose Bengal plate agglutination test (RBPT), the positive and negative diagnostic accuracies of ELISA were 98.92% (92/93) and 96.97% (64/66), respectively. Evaluation of the 153 bovine serum samples showed that the AUC was 0.9974, and compared with those of SAT and RBPT, the positive and negative diagnostic accuracies of ELISA were 98.65% (73/74) and 96.20% (76/79), respectively.Conclusion: The results indicated that rMEP, as a protein antigen, can be used to diagnose brucellosis with high accuracy in both goats and bovines.

The potential value of a pictorial atlas for aid in the visual diagnosis of 123I FP-CIT SPECT scans

2009 ◽  
Vol 48 (04) ◽  
pp. 173-178 ◽  
Author(s):  
H. Ham ◽  
A. Dobbeleir ◽  
P. Santens ◽  
Y. D'Asseler ◽  
I. Goethals
Keyword(s):  
Diagnostic Accuracy ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Interobserver Variability ◽  
Area Under The Curve ◽  
Visual Assessment ◽  
Added Value ◽  
Related Disorder ◽  
Visual Diagnosis ◽  
Spect Images

SummaryThe aim of our study was to evaluate the value of a pictorial atlas of 123I FP-CIT SPECT images for aid in the visual diagnosis. Patients, materials, methods: Sixty patients, of whom 20 were clinically diagnosed as ‘non-parkinsonian’ and 40 as having Parkinson's disease or any related disorder, were included in the study. An atlas consisting of 12 123I FP-CIT SPECT images was constructed first. Validity of the atlas was investigated by performing a receiver operating characteristic (ROC) analysis with the clinical diagnosis as the gold standard. The remaining 48 SPECT images were visually assessed twice by 5 observers, first with and secondly without consulting the atlas, or vice versa. The added value of the atlas was investigated by comparing the diagnostic accuracy and the interobserver variability for both methods. Results: ROC analysis performed on the atlas yielded an area under the curve of 1 for a threshold discriminating between clinically non-parkinsonian and parkinsonian patients that was situated between image 4 and 5 of the atlas. For the diagnostic accuracy, we found that the area under the ROC curve was systematically higher if observers had access to the atlas compared to when they had not (Wilcoxon's test, p<0.05). Also, the interobserver variability was significantly lower when observers used the atlas when compared to when they did not (p = 0.05). Conclusion: Diagnostic accuracy was significantly higher and interobserver variability significantly lower if observers had access to the atlas compared to when they had not. Hence, having a pictorial atlas available may facilitate the visual assessment of 123I FP-CIT SPECT scans.

scholarly journals Cut-off limits of the peak GH response to stimulation tests for the diagnosis of GH deficiency in children and adolescents: study in patients with organic GHD

2016 ◽  
Vol 175 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Chiara Guzzetti ◽  
Anastasia Ibba ◽  
Sabrina Pilia ◽  
Nadia Beltrami ◽  
Natascia Di Iorgi ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Children And Adolescents ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Gh Deficiency ◽  
Tolerance Test ◽  
Gh Secretion ◽  
Insulin Tolerance ◽  
Igf I ◽  
Stimulation Tests

ObjectiveThe diagnosis of GH deficiency (GHD) in children and adolescents is established when GH concentrations fail to reach an arbitrary cut-off level after at least two provocative tests. The objective of the study was to define the optimal GH cut-offs to provocative tests in children and adolescents.DesignRetrospective study in 372 subjects who underwent evaluation of GH secretion. GH and IGF-I were measured by chemiluminescence assay in all samples. Receiver operating characteristic (ROC) analysis was used to evaluate the optimal GH cut-offs and the diagnostic accuracy of provocative tests.MethodsSeventy four patients with organic GHD (GH peak <10μg/L after two provocative tests) and 298 control subjects (GH response >10μg/L to at least one test) were included in the study. The provocative tests used were arginine, insulin tolerance test (ITT) and clonidine. Diagnostic criteria based on cut-offs identified by ROC analysis (best pair of values for sensitivity and specificity) were evaluated for each test individually and for each test combined with IGF-I SDS.ResultsThe optimal GH cut-off for arginine resulted 6.5μg/L, 5.1μg/L for ITT and 6.8μg/L for clonidine. IGF-I SDS has low accuracy in diagnosing GHD (AUC=0.85). The combination of the results of provocative tests with IGF-I concentrations increased the specificity.ConclusionsThe results of the ROC analysis showed that the cut-off limits which discriminate between normal and GHD are lower than those commonly employed. IGF-I is characterized by low diagnostic accuracy.

scholarly journals P302 Diagnostic accuracy of a serum-based biomarker panel for endoscopic activity in ulcerative colitis

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S304-S304
Author(s):  
A HOLMER ◽  
B Boland ◽  
S Singh ◽  
H Le ◽  
J Neill ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Diagnostic Accuracy ◽  
Operating Characteristic ◽  
Mucosal Inflammation ◽  
Serum Samples ◽  
Receiver Operating Characteristic Curves ◽  
Biomarker Panel ◽  
Endoscopic Remission

Abstract Background The endoscopic healing index (EHI, Monitr, Prometheus Biosciences, San Diego, CA) is a serum-based biomarker panel available for identifying mucosal inflammation in Crohn’s disease.[1] We aimed to study its performance for identifying mucosal inflammation in ulcerative colitis. Methods EHI was analysed on serum samples paired with endoscopies from adult patients (≥18 years) participating in a prospective biobank (June 2014 to December 2017). Area under receiver operating characteristic curves (AUROC) were used to assess the accuracy of EHI for endoscopic improvement (EI; Mayo endoscopic sub-score [MES] 0–1) and endoscopic remission (ER; MES 0). Sensitivity for EHI was calculated using a cut-off previously identified for Crohn’s disease which optimised performance for ruling out endoscopic activity (20 points). Alternative cut-offs were explored. Results A total of 114 patients were included, with an overall prevalence of 56% and 44% for EI and ER. The AUROC was 0.79 (95% CI 0.70–0.87) for EI and 0.70 (95% CI 0.61–0.80) for ER. A cut-off of 20 points had a sensitivity of 94% (95% CI 83–99%) for ruling out moderate to severe (MES 2–3) endoscopic activity, and a sensitivity of 84% (95% CI 72–92%) for ruling out mild to severe (MES 1–3) endoscopic activity. A cut off of 40 points or higher had &gt; 90% specificity for ruling in moderate to severe (MES 2–3) or mild to severe (MES 1–3) endoscopic activity. (Table 1) Conclusion EHI has favourable accuracy in identifying the presence of mucosal inflammation in patients with ulcerative colitis. Although it was not developed and validated for ulcerative colitis, further validation is warranted. Reference

scholarly journals Serum miR-101-3p combined with pepsinogen contributes to the early diagnosis of gastric cancer

2020 ◽  
Author(s):  
Weiwei Zeng ◽  
Shuxiang Zhang ◽  
Lei Yang ◽  
Wenchao Wei ◽  
Jie Gao ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Early Diagnosis ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Diagnostic Marker ◽  
Diagnostic Value ◽  
Serum Markers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Submucosal Infiltration ◽  
Normal Controls

Abstract Background: This study aimed to explore the diagnostic value of serum miR-101-3p combined with pepsinogen (PG) on early diagnosis of gastric cancer (GC). Methods: A total of 61 atrophic gastritis (AG) and 86 GC patients, and 50 healthy volunteers were enrolled. The serum expression of miR-101-3p was measured by qRT-PCR. The serum content of carcinoembryonic antigen (CEA) was measured by Electrochemiluminescence immunoassay. The serum contents of PGI and PGII were measured by Enzyme linked immunosorbent assay. The diagnostic value of serum markers on AG and GC was analyzed by receiver operating characteristic (ROC) analysis. Results: The expression of miR-101-3p, the content of PGI and the ratio of PGI/II were significantly decreased, and the content of PGII was significantly increased in AG patients compared with those in normal controls. The changes of the above serum indicators were more obvious in GC patients than those in AG patients. The content of CEA was significantly higher in GC patients than that in AG patients. In addition, the expression of miR-101-3p was negatively associated with the submucosal infiltration in GC patients. MiR-101-3p exhibited high diagnostic value on AG (AUC 0.8493, sensitivity 80.33%, specificity 80%) and GC (AUC 0.8749, sensitivity 72.09%, specificity 86.49%). MiR-101-3p + PGI + PGI/II (AUC 0.856, sensitivity 80.23%, specificity 77.05%) exhibited a high diagnostic value in distinguishing between AG and GC. Conclusions: MiR-101-3p was a potential diagnostic marker for AG and GC. MiR-101-3p + PGI + PGI/II was effective in distinguishing between AG and GC.

scholarly journals 215 Assessment of the Diagnostic Sensitivity and Specificity of Pain Biomarkers in Cattle Using Receiver Operating Characteristic Curves

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 7-8
Author(s):  
Miriam S Martin ◽  
Michael Kleinhenz ◽  
Karen Schwartzkopf-Genswein ◽  
Johann Coetzee
Keyword(s):  
Diagnostic Accuracy ◽  
Receiver Operating Characteristic ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Diagnostic Sensitivity ◽  
Diagnostic Sensitivity And Specificity ◽  
Good Diagnostic Accuracy ◽  
Receiver Operating

Abstract Biomarkers are commonly used to assess pain and analgesic drug efficacy in livestock. However, the diagnostic sensitivity and specificity of these biomarkers for different pain conditions over time have not been described. Receiver operating characteristic (ROC) curves are graphical plots that illustrate the diagnostic ability of a test as its discrimination threshold is varied. The objective of this analysis was to use area under the curve (AUC) values derived from ROC analysis to assess the predictive value of pain biomarkers at specific timepoints. The biomarkers included in the analysis were blood cortisol, salivary cortisol, hair cortisol, infrared thermography (IRT), mechanical nociceptive threshold (MNT), substance P, and outcomes from a pressure/force measurement system and visual analog scale. A total sample size of 7,992 biomarker outcomes were collected from 6 pain studies involving pain associated with castration, dehorning, lameness, and surgery were included in the analysis. Each study consisted of three treatments; pain, no pain, and analgesia. All statistics were performed using statistical software (JMP Pro 14.0, SAS Institute, Inc., Cary, NC). Results comparing analgesia verses pain yielded good diagnostic accuracy (AUC &gt; 0.7; 95% CI: 0.40 to 0.99) for blood cortisol (timepoints 1.5, 2, and 6 hours); IRT (timepoints 6, 8, 12, and 72 hours); and MNT (timepoints 6, 25, and 49 hours). These results indicate that ROC analysis can be a useful indicator of the predictive value of pain biomarkers and certain timepoints seem to yield good diagnostic accuracy while many do not.

scholarly journals Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2496
Author(s):  
Bo Dong ◽  
Gaoqiang Zhang ◽  
Xiaodong Zhang ◽  
Xufei Chen ◽  
Meiling Zhang ◽  
...  
Keyword(s):  
Operating Characteristic ◽  
Characteristic Curve ◽  
Systemic Disease ◽  
Analysis Data ◽  
Spike Protein ◽  
Diagnostic Tools ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Pathogenic Virus ◽  
Feline Coronavirus

Feline coronavirus (FCoV) is a pathogenic virus commonly found in cats that causes a benign enteric illness and fatal systemic disease, feline infectious peritonitis. The development of serological diagnostic tools for FCoV is helpful for clinical diagnosis and epidemiological investigation. Therefore, this study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against FCoV using histidine-tagged recombinant spike protein. FCoV S protein (1127–1400 aa) was expressed and used as an antigen to establish an ELISA. Mice and rabbits immunized with the protein produced antibodies that were recognized and bound to the protein. The intra-assay coefficient of variation (CV) was 1.15–5.04% and the inter-assay CV was 4.28–15.13%, suggesting an acceptable repeatability. iELISA did not cross-react with antisera against other feline viruses. The receiver operating characteristic curve analysis revealed an 86.7% sensitivity and 93.3% specificity for iELISA. Serum samples (n = 107) were tested for anti-FCoV antibodies, and 70.09% of samples were positive for antibodies against FCoV. The iELISA developed in our study can be used to measure serum FCoV antibodies due to its acceptable repeatability, sensitivity, and specificity. Additionally, field sample analysis data demonstrated that FCoV is highly prevalent in cat populations in Fujian province, China.

scholarly journals Serum MALAT1 Assumes Signifying Capacity in Gastric Cancer Diagnosis.

2020 ◽  
Author(s):  
Keqian Zhang ◽  
Tianqi Mao ◽  
Zhicheng He ◽  
Xiaojiao Wu ◽  
Yu Peng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Serum Samples ◽  
Chi Square ◽  
Chi Square Test ◽  
Polymerase Chain

Abstract Background: Gastric cancer (GC) represents one of the most serious cancers worldwide with the increasing mortality. Metastasis associated lung adenocarcinoma transcript 1 (MALAT1), a kind of lncRNAs, has been reported to be involved in the progression of cancers. This study aimed to assess serum expression pattern of MALAT1 and its clinical significance in diagnosis of GC.Methods: Serum specimens were collected from 120 GC patients and 58 healthy individuals. The expression profile of MALAT1 was examined using quantitative real-time polymerase chain reaction (qRT-PCR), and its association with clinical parameters was estimated by chi-square test. The diagnostic value of MALAT1 in GC was evaluated by the receiver operating characteristic (ROC) analysis.Results: Upregulated expression of MALTA1 was found in GC patients compared with the healthy controls (P<0.05). The overexpression of MALAT1 was positively correlated with lymph node metastasis (P=0.041) and TNM stage (P=0.005). An area under the curve (AUC) was 0.897 in ROC analysis, suggesting the high diagnostic value of MALAT1. Conclusion: The expression of MALAT1 was upregulated in GC serum samples, and its expression might serve as a potential diagnostic biomarker in patients with GC.

scholarly journals Seroprevalence of Toxoplasma gondii infection in sheep in Inner Mongolia Province, China

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 11 ◽  
Author(s):  
Xinlei Yan ◽  
Wenying Han ◽  
Yang Wang ◽  
Hongbo Zhang ◽  
Zhihui Gao
Keyword(s):  
Toxoplasma Gondii ◽  
Inner Mongolia ◽  
Animal Husbandry ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Zoonotic Parasite ◽  
Health And Disease ◽  
Toxoplasma Gondii Infection ◽  
Almost All

Toxoplasma gondii is an important zoonotic parasite that can infect almost all warm-blooded animals, including humans, and infection may result in many adverse effects on animal husbandry production. Animal husbandry in Inner Mongolia is well developed, but data on T. gondii infection in sheep are lacking. In this study, we determined the seroprevalence and risk factors associated with the seroprevalence of T. gondii using an indirect enzyme-linked immunosorbent assay (ELISA) test. A total of 1853 serum samples were collected from 29 counties of Xilin Gol League (n = 624), Hohhot City (n = 225), Ordos City (n = 158), Wulanchabu City (n = 144), Bayan Nur City (n = 114) and Hulunbeir City (n = 588). The overall seroprevalence of T. gondii was 15.43%. Risk factor analysis showed that seroprevalence was higher in sheep ≥12 months of age (21.85%) than that in sheep <12 months of age (10.20%) (p < 0.01). Seroprevalence was higher in male sheep (18.76%) than females (12.80%) (p < 0.01). Barn-feeding sheep (23.13%) had higher prevalence than grazing sheep (10.94%) (p < 0.01). The seroprevalence was significantly different in different districts (p < 0.01). This study shows that sheep are exposed to T. gondii in Inner Mongolia, and provides a data reference for public health and disease control.

scholarly journals Proteolyzed Variant of IgG with Free C-Terminal Lysine as a Biomarker of Prostate Cancer

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 817
Author(s):  
Anna Lokshin ◽  
Lyudmila M. Mikhaleva ◽  
Eugene I. Goufman ◽  
Marina N. Boltovskaya ◽  
Natalia B. Tikhonova ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Diagnostic Accuracy ◽  
Conformational Changes ◽  
Operating Characteristic ◽  
Area Under The Curve ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Human Plasminogen ◽  
Total Psa ◽  
Circulating Levels

The differential diagnosis of prostate cancer is problematic due to the lack of markers with high diagnostic accuracy. We previously demonstrated the increased binding of IgG to human plasminogen (PLG) in plasma of patients with prostate cancer (PC) compared to healthy controls. Heavy and light chains of PLG (PLG-H and PLG-L) were immobilized on 96-well plates and the binding of IgG to PLG-H and PLG-L was analyzed in serum from 30 prostate cancer (PC) patients, 30 patients with benign prostatic hyperplasia (BPH) and 30 healthy controls using enzyme-linked immunosorbent assay (ELISA). Our results demonstrate that IgG from PC sera bind to PLG-H but not to PLG-L. This interaction occurred through the free IgG C-terminal lysine (Lys) that becomes exposed as a result of IgG conformational changes associated with proteolysis. Circulating levels of modified IgG with exposed C-terminal Lys (IgG-Lys) were significantly higher in PC patients than in healthy controls and in BPH. We used Receiver Operating Characteristic (ROC) analysis to calculate the sensitivity (SN) and specificity (SP) of circulating IgG-Lys for differentiating PC from BPH as 77% and 90%, respectively. The area under the curve (AUC) was 0.87. We demonstrated that the diagnostic accuracy of circulating levels of IgG-Lys is much higher than diagnostic accuracy of total PSA (tPSA).

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