scholarly journals The Effect of Different Salinity Levels on Chloride Cells of Periophthalmus Waltoni’s Epidermis in Connection with NA + / K + / ATPase ion Transporter and NA + / K + / 2CL Cotransporter using Immunohistochemistry Technique

Author(s):  
Hassan Morovvati ◽  
Mina Amiri Farahani ◽  
Ali Kalantari Hesari ◽  
Kaveh Esfandiyari ◽  
Mohammad Babaei

Abstract Background: The NA+ / K+ / ATPase and NA+ / K+ / 2CL cotransporter are two types of ions transporting proteins that are active in the secretion of chloride in bony fish. So, the level and activity of these transporting proteins are expected to increase in saline water. The aim of this study was to investigate the effect of different salinity levels on chloride cells of Periophthalmus waltoni’s epidermis in connection with NA+ / K+ / ATPase ion transporter and NA+ / K+ / 2CL cotransporter using immunohistochemistry technique.Results: Simultaneous localization of NA+ / K+ / ATPase and NA+ / K+ / 2CL cotransporter showed that both were simultaneously present in epidermal ion cells and were able to react to different salinity levels. Conclusion: The results of this study confirmed the model that states, NA+ / K+ / ATPase and NA+ / K+ / 2CL cotransporters are responsible for the secretion of chloride from the chloride cells of bony fish.

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Fauzul Imron ◽  
Setyo Budi Kurniawan ◽  
Siti Rozaimah Sheikh Abdullah

AbstractLeachate is produced from sanitary landfills containing various pollutants, including heavy metals. This study aimed to determine the resistance of bacteria isolated from non-active sanitary landfill leachate to various heavy metals and the effect of salinity levels on the removal of Hg by the isolated bacterium. Four dominant bacteria from approximately 33 × 1017 colony-forming units per mL identified as Vibrio damsela, Pseudomonas aeruginosa, Pseudomonas stutzeri, and Pseudomonas fluorescens were isolated from non-active sanitary landfill leachate. Heavy metal resistance test was conducted for Hg, Cd, Pb, Mg, Zn, Fe, Mn, and Cu (0–20 mg L− 1). The removal of the most toxic heavy metals by the most resistant bacteria was also determined at different salinity levels, i.e., fresh water (0‰), marginal water (10‰), brackish water (20‰), and saline water (30‰). Results showed that the growth of these bacteria is promoted by Fe, Mn, and Cu, but inhibited by Hg, Cd, Pb, Mg, and Zn. The minimum inhibitory concentration (MIC) of all the bacteria in Fe, Mn, and Cu was > 20 mg L− 1. The MIC of V. damsela was 5 mg L− 1 for Hg and >  20 mg L− 1 for Cd, Pb, Mg, and Zn. For P. aeruginosa, MIC was > 20 mg L− 1 for Cd, Pb, Mg, and Zn and 10 mg L− 1 for Hg. Meanwhile, the MIC of P. stutzeri was > 20 mg L− 1 for Pb, Mg, and Zn and 5 mg L− 1 for Hg and Cd. The MIC of P. fluorescens for Hg, Pb, Mg, and Zn was 5, 5, 15, and 20 mg L− 1, respectively, and that for Cd was > 20 mg L− 1. From the MIC results, Hg is the most toxic heavy metal. In marginal water (10‰), P. aeruginosa FZ-2 removed up to 99.7% Hg compared with that in fresh water (0‰), where it removed only 54% for 72 h. Hence, P. aeruginosa FZ-2 is the most resistant to heavy metals, and saline condition exerts a positive effect on bacteria in removing Hg.


Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 342 ◽  
Author(s):  
Ida Di Mola ◽  
Stefano Conti ◽  
Eugenio Cozzolino ◽  
Giuseppe Melchionna ◽  
Lucia Ottaiano ◽  
...  

Hemp (Cannabis sativa L.) is a multipurpose plant attracting increasing interest as a source for the production of natural fibers, paper, bio-building material and food. In this research we studied the agronomical performance of Cannabis sativa cv. Eletta Campana irrigated with saline water. Under those conditions, we tested the effect of protein hydrolysate (PH) biostimulant application in overcoming and/or balancing deleterious salinity effects. The results of the diverse treatments were also investigated at the physiological level, focusing on photosynthesis by means of a chlorophyll a fluorescence technique, which give an insight into the plant primary photochemical reactions. Four salinity levels of the irrigation solution (fresh water–EC0, and NaCl solutions at EC 2.0, 4.0 or 6.0 dS m−1, EC2, EC4 and EC6, respectively) were combined with 2 biostimulant treatments (untreated (control) or treated with a commercial legume-derived protein hydrolysate (LDPH)). The increasing salinity affected plant photochemistry resulting in lower plant growth and seed production, while the LDPH biostimulant showed a protective effect, which improved crop performance both in control and in salinity conditions. The LDPH treatment improved seeds yield (+38.6% on average of all treated plants respect to untreated plants), as well as residual biomass, relevant in fiber production.


Author(s):  
Qi Wei ◽  
Junzeng Xu ◽  
Linxian Liao ◽  
Yawei Li ◽  
Haiyu Wang ◽  
...  

To reveal the effect of irrigation salinity on soil nitrous oxide (N2O) emission, pot experiments were designed with three irrigation salinity levels (NaCl and CaCl2 of 1, 2.5 and 4 g/L equivalence, Ec = 3.6, 8.1 and 12.7 ds/m), either for 0 kg N/ha (N0) or 120 kg N/ha (N120) nitrogen inputs. N2O emissions from soils irrigated at different salinity levels varied in a similar pattern which was triggered by soil moisture dynamics. Yet, the magnitudes of pulse N2O fluxes were significantly varied, with the peak flux at 5 g/L irrigation salinity level being much higher than at 2 and 8 g/L. Compared to fresh water irrigated soils, cumulative N2O fluxes were reduced by 22.7% and 39.6% (N0), 29.1% and 39.2% (N120) for soils irrigated with 2 and 8 g/L saline water, while they were increased by 87.7% (N0) and 58.3% (N120) for soils irrigated with 5 g/L saline water. These results suggested that the effect degree of salinity on consumption and production of N2O might vary among irrigation salinity ranges. As such, desalinating brackish water to a low salinity level (such as 2 g/L) before it is used for irrigation might be helpful for solving water resources crises and mitigating soil N2O emissions.


2020 ◽  
pp. 1-14
Author(s):  
Alaa Ibrahim ◽  
Jamal Elfaki

A greenhouse experiment was carried out to evaluate the response of tomato (Solanum lycopersicum) to saline water irrigation under soilless and traditional techniques. A special fertigation technique with two different salinity levels (1 dS m-1 and 4 dS m-1) of water was used under different soilless media, namely, perlite, gravel, and pozzolana as inert media, in addition to traditional techniques. Results showed that among the three soilless substrates, perlite medium produced the highest total yields with larger fruit sizes. Furthermore, the perlite medium enabled significant savings in water, compared to gravel (-15%) and pozzolana (-20%). Moreover, the results corroborated the existing knowledge on the tolerance of tomato to brackish water irrigation, since there was no significant difference in yield of plants grown in the soil irrigated with water with salinity levels of 1.1   dSm-1 and 4-5  dS m-1. Plant biometric data revealed a better and quicker development of plants grown in the soilless media compared to those grown in the soil, even in the case of freshwater irrigation.


Author(s):  
M. V. Dlamini ◽  
M. T. Masarirambi

Saline irrigation water is becoming an important water source as fresh water is fast becoming a scarce resource in many areas of the world, including Eswatini, especially in arid and semi-arid regions.  A study to test the response of two varieties of spinach (fordhook giant and mustard) to salinity was conducted in a field pot experiment at the Faculty of Agriculture at the Luyengo Campus of the University of Eswatini.  The treatments were laid in a randomized block design (RCBD).  The experiment consisted of four treatments, each replicated twelve times.  Treatments were salinity levels of 0.0 dS/m, 1.5 dS/m, 2.0 dS/m and 3.5 dS/m.  All the treatments were subjected to similar agronomic practices. Spinach was grown and observed for a period of five weeks.  Plant height was measured and the number of leaves counted weekly throughout the experiment. Significant differences (P < 0.05) between salinity treatments were obtained for plant height beginning in week 2 but were more pronounced in week 3, 4 and week 5.  No significant differences were obtained for the number of leaves.  There were however, clear significant differences between spinach irrigated with none saline irrigation water compared to saline irrigation water.   It was concluded that irrigating spinach with saline water of more than 2.0 dS/m drastically reduce plant growth but not the number of leaves under the conditions of the experiment.


Author(s):  
Jauhari Syamsiah ◽  
Rahayu Rahayu ◽  
Wily Binafsihi

<span>Successful management of saline water could have significant potential for agricultural development in many areas, particularly in freshwater-scarce regions. To date, the effect of salinity on shallot (<em>Allium Cepa</em> L.) yield and growth parameters has not been studied in detail specifically for local varieties cultivated in Inceptisols. Therefore, the present study was designed to evaluate the effects of different levels of irrigation-water salinity (0, 1, 2, and 3 dS</span><span>m</span><sup><span lang="IN">-1</span></sup><span>) on soil chemical properties, the growth, and yield of local shallot varieties. The experiment was conducted in pots using a randomized plot design with two factors and three replications. The results showed that increases in salinity level affected increases soil pH, exchangeable Na percentages</span><span lang="IN">,</span><span> and plant height growth. Nevertheless, bulb number and weight, soil exchangeable Ca and Mg, soil organic carbon, and sodium adsorption ratio (SAR) was not significantly affected. The findings of the present study suggest that the local varieties—<em>Brebes</em> and <em>Purbalingga</em>—with irrigated salinity levels up to 3 dS</span><span>m</span><sup><span lang="IN">-1</span></sup><span>can be tolerated for shallot cultivation in Inceptisols.</span>


Author(s):  
Márcia Cristina de Araújo Pereira ◽  
Carlos Alberto Vieira de Azevedo ◽  
José Dantas Neto ◽  
Mariana de Oliveira Pereira ◽  
Jailton Garcia Ramos ◽  
...  

Brazilian semiarid region is characterized by irregular rainy periods that limits the production of forage feed for the maintenance of agricultural livestock and causes losses in the production. Despite that, semiarid region stands out in the production of forage palm, buffering the impact of drought in the area. Thus, this study aimed at analyzing the effect of different levels of salinity in irrigation water on the production of forage palm in the semiarid region. The experiment was arranged as a complete randomized block, in a 4x3 factorial arrangement and four levels of salinity (0.2, 2.0, 3.8 and 5.6 dS m-1); three cultivars (Miúda, Orelha de Elefante Mexicana and IPA-Sertânia) with 4 replications. The variables evaluated were the following: number of primary, secondary, tertiary and total cladodes, cladode biomass and estimative of threshold salinity (SL). The results showed that irrigation with saline water with an electrical conductivity of 2.0 dS m-1 negatively affected the emission of cladodes of superior orders for all forage palm cultivars. Miúda cultivar presented the best production of fresh phytomass (13.36 kg per plant) under a salinity level of 4.04 dS m-1, followed by Orelha de Elefante Mexicana (12.55 kg per plant) under a salinity level of 2.73 dS m-1 and Baiana (11.56 kg per plant) under a salinity level of 2.17dS m-1.


2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Nuning Argo Subekti ◽  
Hasil Sembiring ◽  
Erythrina ◽  
Dedi Nugraha ◽  
Bhakti Priatmojo ◽  
...  

Abstract. Subekti NA, Sembiring H, Erythrina, Nugraha D, Priatmojo B, Nafisah. 2020. Yield of different rice cultivars at two levels of soil salinity under seawater intrusion in West Java, Indonesia. Biodiversitas 21: 14-20. A tendency to use saline water in rice production is rising in recent years, but the adaptation of variety under saline conditions is still questionable. The aim of the study was to evaluate the response of several rice cultivars on the growth and yield of rice under seawater intrusion in West Java. Two salt-tolerant cultivars (Inpari 34 and Inpari 35), two promising lines (PL-1 and PL-2) and two modern cultivars (Inpari 30 (Ciherang sub1) and Sidenuk) were evaluated in two soil salinity levels. In each farmer's field a Randomized Complete Block Design was applied with three replications per treatment. Results showed that Sidenuk and Inpari 30 produced same yield compared to tolerant varieties and promising lines during dry season under moderate soil salinity. There were not much different among the cultivars tested in terms of plant height and tiller number as well as the biomass and harvest index. However, under high soil salinity seed germination, plant height, number of tillers per plant, above-ground biomass, spikelet number, percent of sterile florets and productivity were significantly affected. Saline tolerant varieties Inpari 34 and Inpari 35 showed their superiority compared to non-tolerant varieties. Both varieties produced 40% higher yield than Inpari 30 (Ciherang sub 1) and Sidenuk.


Author(s):  
Francisco I. F. Oliveira ◽  
Wiliana J. F. de Medeiros ◽  
Claudivan F. de Lacerda ◽  
Antônia L. R. Neves ◽  
Davi R. Oliveira

ABSTRACT Biosaline agriculture is an option for using waters with lower quality. Thus, the objective of this study was to evaluate the growth of ornamental species under irrigation with increasing water salinity levels in two methods of water application. The study was conducted in a greenhouse, in the municipality of Fortaleza, Ceará, Brazil. The treatments were distributed in randomized blocks in split plots, with six levels of water salinity in the plots (0.6 - control, 1.2, 1.8, 2.4, 3.0 and 3.6 dS m-1), two methods of water application in the subplots (localized and sprinkler irrigation) and four ornamental species in the sub-subplots (Catharanthus roseus, Allamanda cathartica, Ixora coccinea and Duranta erecta), with four replicates. Increase in irrigation water electrical conductivity reduced the growth of the studied ornamental species. It was not possible to establish an ideal method for irrigation of ornamental species. Effects of non-localized irrigation on leaf growth were more evident in the species C. roseus and D. erecta, which showed higher specific leaf area.


2020 ◽  
Vol 41 (6) ◽  
pp. 1641-1647
Author(s):  
Mr. Ankush ◽  
◽  
R. Prakash ◽  
R. Singh ◽  
Sachin Kumari ◽  
...  

Aim: The present study was undertaken to evaluate the effect of sewage sludge and saline water irrigation on micronutrients concentration in pearl millet- wheat cropping system. Methodology: The trial was conducted during 2017-2019 at Soil Research Farm, CCS Haryana Agricultural University, Haryana in a factorial random block design with three replications. There were three salinity levels of irrigation water, i.e., canal water (0.35 dS m-1), 8 and 10 dS m-1 EC of saline water; and five fertilization levels, i.e., control (no fertilizer), sewage sludge(5 t ha-1), SS (5 t ha-1) + 50% RDF, SS (5 t ha-1) + 75% RDF and 100 % RDF. However, sewage sludge was applied in Rabi seasons only. Results: Based on pooled analysis of two years, micronutrients (Fe, Mn and Cu) concentration in grain and stover/straw of pearl millet and wheat crops significantly reduced on application of saline water irrigation (ECiw 8 and 10 dS m-1) whereas, Zn concentration was non significantly affected with salinity levels. However, highest concentration of Fe, Mn and Cu was recorded with canal water irrigation. There was a significant increase in micronutrients (Fe, Mn and Cu) concentration in both crops with sewage sludge application in comparison of RDF and control treatments but in case of zinc treatment SS (5 t ha-1) + 75% RDF and 100% RDF were at par with each other. Among treatments, significantly higher concentration of micronutrients was recorded with SS (5 t ha-1) + 75% RDF treatment being at par with SS (5 t ha-1) + 50% RDF. Interpretation: Salinity affects micronutrient availability adversely. However, zinc content was not affected significantly. In such case, sewage sludge application may prove a feasible option that serves as a supplement of nutrients as well as sorting out the problem of dumping waste.


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