scholarly journals Hydrogen Peroxide Can Be a Plausible Biomarker in Cyanobacterial Bloom Treatment

2021 ◽  
Author(s):  
Takashi Asaeda ◽  
Mizanur Rahman ◽  
Helayaye Damitha Lakmali Abeynayaka
Keyword(s):  
Hydrogen Peroxide ◽  
Laboratory Experiments ◽  
Antioxidant Activities ◽  
Nitrogen Concentration ◽  
Cyanobacterial Bloom ◽  
Phosphorus Concentration ◽  
Fixed Nitrogen ◽  
Combined Stresses ◽  
Increasing Trend ◽  
Phosphorous Concentration

Abstract The effect of combined stresses, photoinhibition and nutrient depletion, on the oxidative stress of cyanobacteria was measured in laboratory experiments, to develop the biomass prediction model. Phormidium ambiguum was exposed to various photosynthetically active radiation (PAR) intensities and phosphorous concentrations with fixed nitrogen concentration. The samples were subjected to stress assays by detecting hydrogen peroxide (H2O2) concentration and antioxidant activities of catalase (CAT) and superoxide dismutase (SOD). H2O2 concentration decreased to 30 µmolm-2s-1 of PAR, then increased further with higher PAR intensity. Regarding phosphorus concentration, H2O2 concentration generally decreased with increasing phosphorus concentration. SOD and CAT activities were proportionate to the H2O2 protein-1. No H2O2 concentration detected outside of cells indicated the biological production of H2O2, and the accumulated H2O2 concentration inside cells was parameterized with H2O2 concentration protein-1. Over 30 µmolm-2s-1 of PAR, H2O2 concentration protein-1 had a similar increasing trend with PAR intensity, independently of phosphorous concentration. Meanwhile, with increasing phosphorous concentration, H2O2 protein-1 decreased in a similar pattern regardless of PAR intensity. Protein content decreased with increasing H2O2 gradually up to 4nmol H2O2 mg-1protein, which provides a threshold to restrict the growth of cyanobacteria. With these results. an empirical formula was developed to obtain the cyanobacteria biomass.

scholarly journals Hydrogen peroxide can be a plausible biomarker in cyanobacterial bloom treatment

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Takashi Asaeda ◽  
Mizanur Rahman ◽  
Helayaye Damitha Lakmali Abeynayaka
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activities ◽  
Cyanobacterial Bloom ◽  
Log P ◽  
Phosphorus Concentration ◽  
Total Phosphorus Concentration ◽  
Protein 4.1 ◽  
P Concentration ◽  
Increasing Trend ◽  
Nitrogen Concentrations

AbstractThe effect of combined stresses, photoinhibition, and nutrient depletion on the oxidative stress of cyanobacteria was measured in laboratory experiments to develop the biomass prediction model. Phormidium ambiguum was exposed to various photosynthetically active radiation (PAR) intensities and phosphorous (P) concentrations with fixed nitrogen concentrations. The samples were subjected to stress assays by detecting the hydrogen peroxide (H2O2) concentration and antioxidant activities of catalase (CAT) and superoxide dismutase (SOD). H2O2 concentrations decreased to 30 µmol m−2 s−1 of PAR, then increased with higher PAR intensities. Regarding P concentrations, H2O2 concentrations (nmol L−1) generally decreased with increasing P concentrations. SOD and CAT activities were proportionate to the H2O2 protein−1. No H2O2 concentrations detected outside cells indicated the biological production of H2O2, and the accumulated H2O2 concentration inside cells was parameterized with H2O2 concentration protein−1. With over 30 µmol m−2 s−1 of PAR, H2O2 concentration protein−1 had a similar increasing trend with PAR intensity, independently of P concentration. Meanwhile, with increasing P concentration, H2O2 protein−1 decreased in a similar pattern regardless of PAR intensity. Protein content decreased with gradually increasing H2O2 up to 4 nmol H2O2 mg−1 protein, which provides a threshold to restrict the growth of cyanobacteria. With these results, an empirical formula—protein (mg L−1) = − 192*Log((H2O2/protein)/4.1), where H2O2/protein (nmol mg−1) = − 0.312*PAR2/(502 + PAR2)*((25/PAR)4 + 1)*Log(P/133,100), as a function of total phosphorus concentration, P (µg L−1)—was developed to obtain the cyanobacteria biomass.

scholarly journals Occurrence of a single-species cyanobacterial bloom in a lake in Cyprus: monitoring and treatment with hydrogen peroxide-releasing granules

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Eleni Keliri ◽  
Christia Paraskeva ◽  
Angelos Sofokleous ◽  
Assaf Sukenik ◽  
Dariusz Dziga ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Animal Health ◽  
Cyanobacterial Bloom ◽  
Single Species ◽  
Eutrophic Lake ◽  
Liquid Hydrogen ◽  
Bioactive Metabolites ◽  
Forest Park

AbstractBackgroundExcess loads of nutrients finding their way into waterbodies can cause rapid and excessive growth of phytoplankton species and lead to the formation of cyanobacterial harmful algal blooms (cyano-HABs). Toxic cyanobacteria produce a broad range of bioactive metabolites, some of which are known as cyanotoxins. These metabolites can negatively impact the ecosystem, and human and animal health, thus their presence needs to be closely monitored and mitigated. This study aimed to monitor St. George Lake (Athalassa National Forest Park, Cyprus) for its water quality characteristics, and initiate a new methodology to control the bloom that occurred in the lake during summer 2019, by comparing hydrogen peroxide treatment with novel metallic peroxide granules as source of hydrogen peroxide.ResultsLake monitoring showed that pH, salinity, total dissolved solids and conductivity varied throughout the year, and nutrients concentration was high, indicating a eutrophic lake. The cyanobacteriumMerismopediasp. bloomed in the lake between June and September 2019, comprising up to 99% of the phytoplankton biovolume. The presence of microcystin synthase encoding gene (mcyB, mcyE) was documented, however microcystins were not detected by tandem mass spectroscopy. Treatment with liquid hydrogen peroxide in concentrations 1 to 5 mg L−1had no effect on the phycocyanin fluorescence (Ft) and quantum yield of PSII (Fv/Fm) indicating an ineffective treatment for the denseMerismopediabloom (1 million cells mL−1 ± 20%). Metallic peroxide granules tested for their H2O2releasing capacity in St. George Lake water, showing that CaO2released higher H2O2concentration and therefore have better mitigation efficiency than MgO2granules.ConclusionThe present study highlights the importance of monitoring several water parameters to conclude on the different actions to be taken to limit eutrophication in the catchment area. The findings demonstrated that testing for the presence of genes involved in cyanotoxin production may not be sufficient to follow cyanotoxins in the water, therefore it should be accompanied with analytical confirmation. Treatment experiments indicated that slow release of H2O2from peroxide granules may be an alternative to liquid hydrogen peroxide when applied in appropriate doses, but further investigation is needed before it is applied at the field.Graphic Abstract

scholarly journals Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

2020 ◽  
Vol 12 (2) ◽  
pp. 177-183
Author(s):  
Idorenyin Nwaehujor ◽  
Samuel Akande ◽  
Olubunmi Atolani ◽  
Gabriel Olatunji
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Membrane ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Proteinase Inhibition

Inflammation has stimulated significant worldwide scientific interest because of its implication in many human diseases. Most inflammations are caused by reactive oxygen species or free radicals. Annona muricataleaf extracts were investigated for their in-vitroantioxidant and anti-inflammatory potentials. Annona muricataleavesweredried at room temperature, blended using a mill.and extracted with solvents of varying degree of polarities. The solventsused were hexane, ethyl acetate,and ethanol. After sequential extraction, the crude extracts were examined for their in-vitroanti-inflammatory activities on lipoxygenase inhibition, proteinase inhibition, albumin denaturation inhibition,and red blood cell membrane stabilization assays,while the antioxidant activities were examined using DPPH, ABTS and hydrogen peroxide assays. The results showed that the ethanol extract had significantlyhigher albumin denaturation inhibition activity at 500 μg/mL (p < 0.01). The activity of all the extracts on proteinase inhibition decreased with the increase in concentration of the extracts. Indomethacin (standard), ethanol extract,and ethyl acetate extract exhibited a dose dependent increase in lipoxygenase activity. The ethanol extract showed highred blood cell membrane stabilization activity at 500 μg/mL and the activity was comparable with that of the standard (diclofenac). Hydrogen peroxide scavenging activity of the extracts and standard (Vitamin C) were comparable at 20 –100 μg/mL. The ethanol extract showed significantly higher(p < 0.01) DPPH radical scavenging activity compared with other extracts. A similar trend was also observed for ABTS radical scavenging activity. Generally,the ethanol extract exhibited higher anti-inflammatory and antioxidant activities in most of the assays, this could be attributed to the polar compounds present in the extract.

scholarly journals Reducing Nitrogen and Phosphorus Losses from Different Crop Types in the Water Source Area of the Danjiang River, China

2019 ◽  
Vol 16 (18) ◽  
pp. 3442 ◽  
Author(s):  
Mengjing Guo ◽  
Tiegang Zhang ◽  
Jing Li ◽  
Zhanbin Li ◽  
Guoce Xu ◽  
...  
Keyword(s):  
Total Nitrogen ◽  
Total Phosphorus ◽  
Unit Area ◽  
Nitrogen Concentration ◽  
Source Area ◽  
Water Source ◽  
Nutrient Loss ◽  
Phosphorus Concentration ◽  
Nitrogen And Phosphorus ◽  
Bare Land

Nitrogen and phosphorus are essential for plant growth and are the primary limiting nutrient elements. The loss of nitrogen and phosphorus in agricultural systems can cause the eutrophication of natural water bodies. In this paper, a field simulated rainfall experiment was conducted in a typical small watershed of the Danjiang River to study the nutrient loss process of nitrogen and phosphorus in slope croplands subjected to different crops and tillage measures. The characteristics of the runoff process and nutrient migration of different slope treatments were studied, which were the bare-land (BL, as the control), peanut monoculture (PL), corn monoculture (CL), bare land (upper slope) mixed with peanut monoculture (lower slope) (BP), corn and peanut intercropping (TCP), corn and soybean intercropping (TCS), downslope ridge cultivation (BS) slope, and straw-mulched (SC), respectively. The results showed that the runoff of CL, SC, TCS, BS, BP, PL and TCP slope types were 93%, 75%, 51%, 39%, 28%, 12%, and 6% of the those of the bare land, respectively. The total nitrogen concentration in runoff on different slope types decreased in the order of BP > PL > BS > SC > TCP > BL > CL > TCS. The BL was characterized with the highest NRL-TN (the loss of total nitrogen per unit area), with the value of 1.188 kg/hm2, while those of the TCP is the smallest with the value of 0.073 kg/hm2. The total phosphorus concentration in runoff decreasd in the order of BS > BP > PL > BL > TCP > SC > CL > TCS. The PRL-TP (the loss of total phosphorus per unit area) of BL is the largest (0.016 kg/hm2), while those of TCP is the smallest (0.001 kg/hm2). These indicate that the loss of nitrogen is much higer than that of phosphorus. The loss of nitrogen in runoff is dominated by nitrate nitrogen, which accounts for 54.4%–78.9% of TN. Slope croplands in the water source area should adopt the tillage measures of TCP and PL.These measures can reduce 85% of the runoff of nitrogen and phosphorus compared to the bare land. The results may assist in agricultural non-point source pollution control and help promote improved management of the water environment in the Danjiang River’s water source area.

Effects of nitrogen fertilizer, plant population and irrigation on sugar beet: II. Nutrient concentration and uptake

1971 ◽  
Vol 76 (2) ◽  
pp. 269-275 ◽  
Author(s):  
A. P. Draycott ◽  
M. J. Durrant
Keyword(s):  
Sugar Beet ◽  
Nitrogen Fertilizer ◽  
Nutrient Concentration ◽  
Field Experiments ◽  
Nitrogen Concentration ◽  
Plant Size ◽  
Plant Population ◽  
Sugar Yield ◽  
Phosphorus Concentration ◽  
Wide Range

SUMMARYThe concentration of nitrogen, phosphorus, potassium, sodium, calcium and magnesium was measured in the dry matter of sugar beet from four field experiments (1966–9). All combinations of four amounts of nitrogen fertilizer (0–1·8 cwt/acre), four plant populations (8800–54000 plants/acre) and irrigation were tested, which gave a wide range of plant size and yield. Nutrient concentration and uptake by the crop were also greatly affected by the treatments.Nitrogen fertilizer and irrigation increased uptake of nitrogen by the crop but increasing the plant population had little effect on uptake and decreased the concentration of nitrogen. Sugar yield was related to the total nitrogen concentration in tops and roots and to uptake. There were optimal values of nitrogen concentration for maximal sugar yield, but the optima were greatly affected by plant population. Leaf colour was a good guide to nitrogen concentration.Phosphorus concentration was affected little by the treatments but cation concentrations were greatly affected. In general, uptake of all the elements was increased by all treatments – the exception was sodium, which decreased as the plant population increased but this was balanced to somo extent by increased potassium uptake.

scholarly journals Nutrient enrichment effect on macroinvertebrates in a lowland stream of Argentina

2015 ◽  
Vol 105 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Agustina Cortelezzi ◽  
Carolina Ocón ◽  
María V. López van Oosterom ◽  
Rosana Cepeda ◽  
Alberto Rodrigues Capítulo
Keyword(s):  
Climate Change ◽  
Global Climate Change ◽  
Global Climate ◽  
Nitrogen Concentration ◽  
Nutrient Concentrations ◽  
Phosphorus Concentration ◽  
Lowland Stream ◽  
Pampean Streams ◽  
High Concentrations ◽  
Intensive Land Use

ABSTRACT One of the most important effects derived from the intensive land use is the increase of nutrient concentration in the aquatic systems due to superficial drainage. Besides, the increment of precipitations in South America connected to the global climate change could intensify these anthropic impacts due to the changes in the runoff pattern and a greater discharge of water in the streams and rivers. The pampean streams are singular environments with high natural nutrient concentrations which could be increased even more if the predictions of global climate change for the area are met. In this context, the effect of experimental nutrient addition on macroinvertebrates in a lowland stream is studied. Samplings were carried out from March 2007 to February 2009 in two reaches (fertilized and unfertilized), upstream and downstream from the input of nutrients. The addition of nutrients caused an increase in the phosphorus concentration in the fertilized reach which was not observed for nitrogen concentration. From all macroinvertebrates studied only two taxa had significant differences in their abundance after fertilization: Corbicula fluminea and Ostracoda. Our results reveal that the disturbance caused by the increase of nutrients on the benthic community depends on basal nutrients concentration. The weak response of macroinvertebrates to fertilization in the pampean streams could be due to their tolerance to high concentrations of nutrients in relation to their evolutionary history in streams naturally enriched with nutrients. Further research concerning the thresholds of nutrients affecting macroinvertebrates and about the adaptive advantages of taxa in naturally eutrophic environments is still needed. This information will allow for a better understanding of the processes of nutrient cycling and for the construction of restoration measures in natural eutrophic ecosystems.

Sulphur and phosphorus fertilizers increase the yield of barrel medic (Medicago truncatula) five-fold in native pasture on a traprock soil

1989 ◽  
Vol 29 (4) ◽  
pp. 527 ◽  
Author(s):  
NM Clarkson ◽  
IF Swann ◽  
NP Chaplain
Keyword(s):  
Medicago Truncatula ◽  
Elemental Sulfur ◽  
Nitrogen Concentration ◽  
Nutrient Concentrations ◽  
Phosphorus Concentration ◽  
First Year ◽  
Yield Increase ◽  
Barrel Medic ◽  
Yield And Quality ◽  
Native Pasture

A single application of sulfur and phosphorus in the first year produced a 5-fold yield increase in barrel medic (Medicago truncatula cv. Jemalong), every year for 3 years, on traprock country in southern inland Queensland. In the first year, the yield of medic increased from 400 (untreated) to 1300 kg/ha with sulfur alone (at 40 kg/ha), and to 2300 kg /ha with sulfur and phosphorus together (both at 40 kg/ha). In the second and third years, sulfur and phosphorus together increased yields from 300 to 1400 kg/ha, and from 800 to 4000 kg/ha, respectively. The experiment tested factorial combinations of sulfur and phosphorus, both at 0, 10 and 40 kg/ha, 3 sources of sulfur (gypsum, superphosphate fortified with elemental sulfur and called 'super and sulfur', and elemental sulfur) and agricultural limestone at 2500 kg/ha. Seed and fertiliser were broadcast into a native pasture at the start of the experiment. Elemental sulfur was inferior to gypsum and 'super and sulfur' in the first year, but equally effective thereafter. There was no response to lime. Fertilising with sulfur and phosphorus together increased the sulfur concentration in medic tops from 0.10 to 0.23%, the phosphorus concentration from 0.19 to 0.23%, and the nitrogen concentration from 2.12 to 2.82%. Nutrient concentrations in plant and soil were examined as an aid to diagnosing deficiencies at other sites. We concluded that adequately fertilising medic with sulfur and phosphorus can greatly improve pasture yield and quality, and allow higher stocking rates on traprock country.

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