scholarly journals Role of Bio-based Synthesized Nano Zinc Oxide in Ameliorating the Deleterious Effects Caused by Lead in Vigna radiata L.

2021 ◽  
Author(s):  
RAVI KUMAR YADAV ◽  
Narsingh Bahadur Singh ◽  
AJEY SINGH ◽  
VIJAYA YADAV ◽  
KM NIHARIKA ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Vigna Radiata ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Zno Nps ◽  
Positive Effects ◽  
Nano Zinc Oxide ◽  
Transmission Electron ◽  
Nano Zinc

Abstract The present work describes the bio-based synthesis (green) and characterization of Zinc oxide nanoparticles (ZnO NPs) using leaf extract of Tridax, the synthesized nanoparticles were used to study their beneficial effect in the growth and metabolism of Vigna radiata. Zinc oxide nanoparticles (ZnONP) were characterized using X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), High-Resolution Transmission Electron Microscopy (HR-TEM), and Ultraviolet–visible spectroscopy (UV–Vis spectra). Growth of V. radiata seedlings was measured in terms of shoot length (SL) and root length (RL) were treated 20 and 40 mg/L concentrations of green synthesized ZnO NPs, and constant concentration (50 mg/L) of PbCl2. These studies have shown the effect of ZnO NPs in the stimulation of growth as well as physiological and biochemical parameters. Vigna seedlings showed positive effects depending upon the increasing concentrations of ZnO NPs. This study suggests that ZnO NPs can be effectively used to ameliorate the toxicity of Pb in Vigna plants.

scholarly journals The Root Endophytic Fungus Serendipita indica Decreased the Phytotoxicity of Zinc Oxide Nanoparticles to Alfalfa (Medicago sativa L.)

2021 ◽  
Author(s):  
Leila Tabande ◽  
Mozhgan Sepehri ◽  
Jafar Yasrebi ◽  
Mehdi Zarei ◽  
Reza Ghasemi-Fasaei ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Plant Growth ◽  
Zinc Oxide Nanoparticles ◽  
Sinorhizobium Meliloti ◽  
Dry Weight ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Shoot Dry Weight ◽  
Nano Zinc Oxide ◽  
Nano Zinc

Abstract Zinc oxide nanoparticles (ZnO-NPs) are among the most commonly used nano-fertilizers (NF). However, elevated levels of ZnO-NPs in soil may affect plant growth and development due to its potential toxicity when accumulated in large amounts in plant tissues. This research was conducted using an in situ rhizobox system with the aims of evaluating Zinc uptake from nano-zinc oxide amended rhizosphere soil by alfalfa plant and the effect of plant growth promoting microorganisms on alleviating the phytotoxicity of ZnO-NPs. Treatments included microbial inoculations (Sinorhizobium meliloti, Serendipita indica) and different ZnO-NPs concentrations (0, 400 and 800 mg Kg− 1) with three replications. The results indicated that S. indica minimized the phytotoxicity of ZnO-NPs to alfalfa by enhancing growth rate and decreasing Zinc (Zn) translocation from root to shoot. Compared with plants inoculated with S. meliloti, co-inoculation with S. indica increased the shoot dry weight by 18.33% and 8.05% at 400 and 800 mg Kg− 1ZnO-NPs. However, at the highest level of ZnO-NPs (800 mg kg− 1), root inoculation of S. indica and S. indica + S. meliloti decreased Zn transfer factor by 60.2% and 44.3% compared to S. meliloti, respectively. Furthermore, a distinct relation between tolerance of S. indica-colonized plant to ZnO-NPs and the ability of S. indica in inhibiting or retarding degradation of polyunsaturated lipids through prevention of excess reactive oxygen species formation was observed. Malondialdehyde content of inoculated plants with S. indica either alone or in combination with S. meliloti was significantly lower than non-inoculated plants (p < 0.01). Zn-induced oxidative stress was mitigated by S. indica through enhanced activities of catalase and peroxidase enzymes. The findings of the present study indicate the potential use of endophytes fungus S. indica for ensuring food safety and security, and human health in heavy metal–polluted soil by reducing the phytoavailability of heavy metals in the aerial parts of the host plants.

Preparation and Antibacterial Performances of Electrocatalytic Zinc Oxide Nanoparticles with Diverse Morphologies

2021 ◽  
Vol 17 (9) ◽  
pp. 1824-1829
Author(s):  
Junlin Li ◽  
Xiangfei Li ◽  
Dong Liang ◽  
Xiaojuan Zhang ◽  
Qing Lin ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Surface Area ◽  
Zinc Oxide Nanoparticles ◽  
Average Particle Size ◽  
Three Dimensional ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Average Particle ◽  
X Ray Diffraction ◽  
Zno Nps

This study exploits the potential of zinc oxide nanoparticles (ZnO-NPs) with diverse morphologies as catalysts and antibacterial agent. Spherical ZnO-NPs, rod-shaped ZnO-NPs and flower-shaped ZnO-NPs were prepared by microemulsion method, solvent heat method and hydrothermal method, respectively. The structural characterizations of samples were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. XRD results revealed the formation of spherical ZnO-NPs, rod-shaped ZnO-NPs and flower-shaped ZnO-NPs were all wurtzite crystal structure. SEM results showed that spherical ZnO-NPs had an average particle size of 30–40 nm, rod-shaped ZnO-NPs were about 500 nm long and 100 nm wide with obvious hexagonal crystals. Flower-shaped ZnO-NPs had a three-dimensional appearance with obvious petals. Results of electrochemical HER (Hydrogen evolution reaction) experiments revealed that spherical ZnO-NPs exhibited the highest electrocatalytic activity at the lowest potential voltage due to their largest specific surface area. The antibacterial property of ZnO-NPs samples were studied by the optical density method and disc diffusion method. All samples had antibacterial effects against E. coli. and flower-shaped ZnO-NPs showed the best antibacterial activity due to the largest surface area in comparison with spherical ZnO-NPs and rod-shaped ZnO-NPs, which promised the maximum Zn2+ release as bactericide mechanism that registered in the case of different ZnO-NPs morphologies.

scholarly journals Bioactivities of Geranium wallichianum Leaf Extracts Conjugated with Zinc Oxide Nanoparticles

Biomolecules ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 38 ◽  
Author(s):  
Banzeer Ahsan Abbasi ◽  
Javed Iqbal ◽  
Riaz Ahmad ◽  
Layiq Zia ◽  
Sobia Kanwal ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Energy Dispersive ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
X Ray ◽  
Transmission Electron

This study attempts to obtain and test the bioactivities of leaf extracts from a medicinal plant, Geranium wallichianum (GW), when conjugated with zinc oxide nanoparticles (ZnONPs). The integrity of leaf extract-conjugated ZnONPs (GW-ZnONPs) was confirmed using various techniques, including Ultraviolet–visible spectroscopy, X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, energy-dispersive spectra (EDS), scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The size of ZnONPs was approximately 18 nm, which was determined by TEM analysis. Additionally, the energy-dispersive spectra (EDS) revealed that NPs have zinc in its pure form. Bioactivities of GW-ZnONPs including antimicrobial potentials, cytotoxicity, antioxidative capacities, inhibition potentials against α-amylase, and protein kinases, as well as biocompatibility were intensively tested and confirmed. Altogether, the results revealed that GW-ZnONPs are non-toxic, biocompatible, and have considerable potential in biological applications.

LASER-INDUCED PHOTOLUMINESCENT STUDIES OF Al-DOPED ZINC OXIDE NANOPARTICLES

2010 ◽  
Vol 09 (05) ◽  
pp. 439-445
Author(s):  
DHIRAJ KUMAR ◽  
SUNIL KUMAR ◽  
H. S. BHATTI
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Nitrogen Laser ◽  
X Ray Diffraction ◽  
Electron Microscopic ◽  
Time Resolved ◽  
X Ray ◽  
Aluminum Ions ◽  
Transmission Electron

In this paper, addition of aluminum in zinc oxide is incorporated using low-temperature chemical synthesis route. Aluminum ions help in crystallization of zinc oxide nanoparticles. Characterization of the synthesized nanoparticles of zinc oxide has been done using Transmission electron microscope (TEM), and X-ray diffraction (XRD) analysis, Energy-resolved photoluminescence (PL) spectra and Time-resolved laser-induced photoluminescence (TRPL) at room temperature. Transmission electron microscopic observations and X-Ray diffraction studies indicate highly crystalline nature and particle size of the order of 20 nm in ZnO:Al . Time-resolved laser-induced photoluminescence measurements have been done using pulsed nitrogen laser as an excitation source, operated at wavelength 337.1 nm and having high peak output power of 1 MW. The results show that at higher concentrations of Al doping in host ZnO phosphor, emission intensity is more by several orders of magnitude and lifetime shortening indicates that these nanoparticles are more efficient as compared with lower concentrations of dopant.

Preparation of Template Free Zinc Oxide Nanoparticles Using Sol–Gel Chemistry

2008 ◽  
Vol 8 (8) ◽  
pp. 4224-4226 ◽  
Author(s):  
Mohan Raja ◽  
A. M. Shanmugaraj ◽  
Sung Hun Ryu
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Sodium Hydroxide Solution ◽  
Sol Gel ◽  
Oxide Nanoparticles ◽  
Zno Nanoparticle ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Controlled Morphology ◽  
Template Free

Zinc oxide (ZnO) nanoparticle has been synthesized via sol–gel chemistry using zinc acetate as a precursor with sodium hydroxide solution through microemulsion technique. X-ray diffraction (XRD) and transmission electron microscopy (TEM) studies reveal that the resultant zinc oxide nanoparticles are wurtzite type with the controlled morphology of nano triangular and half ellipsoid structures. Room temperature photoluminescence measurement of zinc oxide nanoparticles exhibits a strong green band at around 375 nm with excitation energy of 3.30 eV indicating oxygen vacancies on the surface of the nanoparticles.

Beneficial Effects of Zinc Oxide Nanoparticles on Plant Regeneration of Vetiver Grass (Vetiveria zizanioides L. Nash)

2017 ◽  
Vol 866 ◽  
pp. 25-28
Author(s):  
Chonnikarn Khunchuay ◽  
Kanokporn Sompornpailin
Keyword(s):  
Zinc Oxide ◽  
Plant Regeneration ◽  
Zinc Oxide Nanoparticles ◽  
Shoot Length ◽  
Optimal Concentration ◽  
Oxide Nanoparticles ◽  
Vetiver Grass ◽  
Vetiveria Zizanioides ◽  
Zno Nps ◽  
Positive Effects

Zinc is an essential micronutrient element for plant. Nowadays, Zinc oxide nanoparticles (ZnO NPs) are widely used and have been shown adverse effects to plant physiology. The optimal concentration of ZnO NPs added in plant regeneration medium (PRM) is studied in this experiment. Six-weeks old calli induced from the aseptic axillary buds of vetiver grass were cultured in PRM with various concentrations of ZnO NPs (5, 10, 20, 40 mgL-1). PRM containing 5, 10 mgL-1 ZnO NPs showed the highest percentage of plant regeneration frequency (95%) but did not have a statistic difference with other treatments. However, the averages of shoot length regenerated under both concentrations were 1.32-1.33 cm. These averages of shoot length had statistically different from averages of shoot length of the others. Moreover, calli regenerated under PRM containing 5 mgL-1 ZnO NPs condition showed the highest average number of shoots per callus (9.75 shoots) with a statistic difference. ZnO NPs at 20-40 mgL-1 in the PRM did not enhance the percentage of plant regeneration, but retarded calli development and shoot elongation. The optimal concentration of ZnO NPs in PRM has positive effects on regeneration and development of vetiver grass under tissue culture condition.

scholarly journals Laboratory and Commercial Synthesized Zinc Oxide Nanoparticles Adsorption onto Coconut Husk: Characterization, Isotherm, Kinetic, and Thermodynamic Studies

2020 ◽  
Vol 11 (1) ◽  
pp. 7871-7889
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Batch Adsorption ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Maximum Capacity ◽  
Maximum Percentage ◽  
Coconut Husk ◽  
Transmission Electron ◽  
Percentage Removal

The accelerating application of zinc oxide nanoparticles (ZnO-NPs) has called for attention to their potential environmental and human health risks. This work aimed to investigate the sorption efficiency of laboratory and commercial synthesized nanocrystalline zinc oxide onto raw coconut husk in a batch adsorption study. Characterization of samples was performed by employing spectroscopies techniques such as X-ray Diffraction Spectroscopy, Field Emission Scanning Electron Microscopy, Transmission Electron Spectroscopy, Fourier transform IR Spectroscopy, and Brunauer–Emmett–Teller. A spherical shaped nanocrystalline ZnO with a mean crystallite and particle size of 14.7 nm and 24 nm by XRD and TEM was synthesized as compared to the commercial ZnO-NPs of size < 50 nm. The maximum percentage removal of 88% (0.13 mg/g) and 90% (0.16 mg/g) for laboratory synthesized and commercial ZnO-NPs respectively was recorded at an optimum contact time of 80 minutes. The data also indicated 2.0 g sorbent mass and pH of 8 as the optimum conditions for maximum percentage removal of these nanoparticles. Both Langmuir and Freundlich models fitted best for laboratory synthesized ZnO-NPs with a maximum capacity of 0.797 mg/g, whereas Langmuir isotherm model alone with a maximum capacity of 0.710 mg/g fitted well for commercial ZnO-NPs. The n-value from the Freundlich model, as well as separation factor (RL) were greater than unity suggesting a favorable adsorption process. The study obeyed pseudo-second-order, which was exothermic with a high degree of freedom of sorbent-sorbate interaction. The results suggested that coconut husk is potentially scalable for removing ZnO-NPs from wastewater.

scholarly journals Antibacterial Action and Target Mechanisms of Zinc Oxide Nanoparticles Against Bacterial Pathogens

2021 ◽  
Author(s):  
Carolina Rosai Mendes ◽  
Guilherme Dilarri ◽  
Carolina Froes Forsan ◽  
Vinícius de Moraes Ruy Sapata ◽  
Paulo Renato Matos Lopes ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Bacterial Resistance ◽  
Membrane Integrity ◽  
Antimicrobial Properties ◽  
Oxide Nanoparticles ◽  
X Ray Diffraction ◽  
Zno Nps ◽  
Z Ring ◽  
Ft Ir

Abstract Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. The study characterized ZnO NPs using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of clinically bacteria Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa was evaluated by REMA after exposure to the ZnO NP at concentrations from 0.2 to 1.4 mM. Sensitivity was achieved at 0.6 mM for the Gram-negatives and 1.0 mM for Gram-positives cells. The effect of ZnO NPs on the membrane integrity and in the interference of cell division was investigated by its effect on the divisional ring, through fluorescence microscopy assays using B. subtilis (amy::pspac-ftsZ-gfpmut1) expressing FtsZ-GFP. Results showed that ZnO NPs did not interfere with the assembly of the divisional Z-ring. However, 70% of the cells showed damage in the cytoplasmic membrane after 15 min of exposure to the ZnO NPs. Electrostatic forces, production of Zn2+ ions, generation of reactive oxygen species were described as pathways of bactericidal action by ZnO. Thus, understanding bactericidal MOA can produce predictive models to prevent bacterial resistance and lead to further research.

Size-Dependent Biological Activities of Fluorescent Organosilane-Modified Zinc Oxide Nanoparticles

2020 ◽  
Vol 16 (2) ◽  
pp. 137-152
Author(s):  
Mariana Buşilă ◽  
Aurel Tăbăcaru ◽  
Viorica Muşsat ◽  
Bogdan Ştefan Vasile ◽  
Ionela Andreea Neaşu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Zinc Oxide ◽  
Cancer Cells ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cytotoxic Activities ◽  
Zno Nps ◽  
Size Dependent ◽  
Transmission Electron

Surface modification of zinc oxide nanoparticles (ZnO NPs) is a strategy to tune their biocompatibility. Herein we report on the synthesis of a series of fluorescent ZnO NPs modified with 2–10% (3-glycidyloxypropyl)trimethoxysilane (GPTMS) to investigate the fluorescence properties and to explore their applications in microbiology and biomedicine. The obtained ZnO NPs were characterized by X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM) and Fourier transform infrared spectroscopy (FTIR). Size reduction occurred from ca. 13 nm in unmodified ZnO to 3–4 nm in silane-modified samples and fluorescence spectra showed size-dependent variation of the photoemission bands' intensity. The antibacterial and cytotoxic activities were investigated on Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria, and in ovarian (A2780) and prostate (PC3) cancer cells by tetrazolium/formazan-based methods. The antibacterial effect was higher for E. coli than S. aureus, while the cytotoxic activity was similar for both cancer cells and varied with the particle size. Cell death by apoptosis, and/or necrosis versus autophagy, were explored by flow cytometry using an Annexin V based-method and transmission electron microscopy (TEM). The main mechanism of ZnO NPs toxicity may involve the generation of reactive oxygen species (ROS) and the induction of apoptosis or autophagy. This work revealed the potential utility of GPTMS-modified ZnO NPs in the treatment of bacterial infection and cancer.

scholarly journals Zinc oxide nanoparticles alleviate the arsenic toxicity and decrease the accumulation of arsenic in rice (Oryza sativa L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shiwei Yan ◽  
Fan Wu ◽  
Song Zhou ◽  
Jianhao Yang ◽  
Xianjin Tang ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oryza Sativa L ◽  
Dry Weight ◽  
Oxide Nanoparticles ◽  
Rice Seedlings ◽  
Nano Zinc Oxide ◽  
Class 1 ◽  
Nano Zinc ◽  
Different Levels

Abstract Background Rice is particularly effective, compared to other cereals, at accumulating arsenic (As), a nonthreshold, class 1 human carcinogen in shoot and grain. Nano-zinc oxide is gradually used in agricultural production due to its adsorption capacity and as a nutrient element. An experiment was performed to explore the effects of zinc oxide nanoparticles (nZnO) on arsenic (As) toxicity and bioaccumulation in rice. Rice seedlings were treated with different levels of nZnO (0, 10, 20, 50, 100 mg/L) and As (0, and 2 mg/L) for 7 days. Results The research showed that 2 mg/L of As treatment represented a stress condition, which was evidenced by phenotypic images, seedling dry weight, chlorophyll, and antioxidant enzyme activity of rice shoot. The addition of nZnO (10–100 mg/L) enhanced the growth and photosynthesis of rice seedlings. As concentrations in the shoots and roots were decreased by a maximum of 40.7 and 31.6% compared to the control, respectively. Arsenite [As (III)] was the main species in both roots (98.5–99.5%) and shoots (95.0–99.6%) when exposed to different treatments. Phytochelatins (PCs) content up-regulated in the roots induced more As (III)-PC to be complexed and reduced As (III) mobility for transport to shoots by nZnO addition. Conclusion The results confirmed that nZnO could improve rice growth and decrease As accumulation in shoots, and it performs best at a concentration of 100 mg/L.

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