Bee's Honeys With High Concentration of Dicarbonyl Molecules Are Rich in Vitamin C and Deficient in Hydrogen Peroxide

2020 ◽  
Author(s):  
Wed Mohammed Ali ALaerjani ◽  
Amal Ahmed Ali Al-Musa ◽  
Mohammed Elimam Mohammed
Keyword(s):  
Hydrogen Peroxide ◽  
Vitamin C ◽  
Physicochemical Properties ◽  
Uv Radiation ◽  
Oxygen Pressure ◽  
High Concentration ◽  
P Values ◽  
Acacia Honey ◽  
The Mean ◽  
Mean Concentration

Abstract The altitude is well known to affect the temperature, parometric and oxygen pressure and the amount of UV radiation which affect the bees and the physicochemical properties of bee's honey. This study investigated the relations between the concentration of hydrogen peroxide, vitamin C and dicarbonyl molecules in honey samples from different floral origins and altitudes. Ten Ziziphus and twenty Acacia honey samples were collected directly from their bee farms. The hydrogen peroxide and vitamin C were measured using redox titartions while the dicarbonyl molecules concnetration was determined spectrophotometerically. The results were statisticaly analyzed by the ANOVA and t-test of the SPSS. The mean concentration of vitamin C in the Acacia and Ziziphus honey samples were 275.14± 82.3 and 239.16± 91.5 mg/100g, respectively. The mean hydrogen peroxide percentages in the Acacia and Ziziphus honey samples were 2.66± 0.81 and 4.94± 1.85%, respectively. The mean concentrations of the dicarbonyl molecules in the Acacia and Ziziphus were 324.62± 291.03 and 115.75± 94.9 mg/Kg, respectively. Significant variations were reported in the case of the hydrogen peroxide and the dicarbonyl molecules concentrations in the Acacia and Ziziphus honeys (p- values = 0.004 and 0.007, respectively). The altitude significantly afected the studied parameters. Honeys rich in dicarbonyl molecules have a high concentration of vitamin C and low content of hydrogen peroxide.

Mineral content of honeybee-collected pollen from southern New South Wales

2002 ◽  
Vol 42 (8) ◽  
pp. 1131 ◽  
Author(s):  
D. C. Somerville ◽  
H. I. Nicol
Keyword(s):  
Mineral Content ◽  
New South ◽  
Single Species ◽  
Prunus Dulcis ◽  
High Concentration ◽  
Minor Elements ◽  
South Wales ◽  
Low Concentrations ◽  
The Mean ◽  
Mean Concentration

The mineral content of honeybee-collected pollen from 34 floral species was analysed for 10 elements. The mean concentrations (mg/kg) of major and minor elements were the following: potassium (K) 5530, phosphorus (P) 4600, sulfur (S) 2378, calcium (Ca) 1146, magnesium (Mg) 716, sodium (Na) 82, iron (Fe) 67, zinc�(Zn) 58, manganese (Mn) 33 and copper (Cu) 12. Close correlations existed between Mn and Cu, P and S, K and S and K and Zn. Single species demonstrated similar element profiles. Echium plantagineum pollen had a high mean concentration of P (7411 mg/kg) and S (3133 mg/kg) when compared with the mean of the total; Brassica�napus pollen had high concentration of Mg (1400 mg/kg) and Ca (1750 mg/kg) and low concentration of Fe (27 mg/kg); Hypochoeris radicata had low concentrations of 6 elements — Fe (4.5 mg/kg), Zn (20 mg/kg), Mg�(240 mg/kg), S (1400 mg/kg), P (2066 mg/kg) and K (2433 mg/kg). Asphodelus fistulosus had the highest concentration of K at 38 000 mg/kg, the next highest value of 8200 mg/kg being for Prunus dulcis.

scholarly journals Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep

2006 ◽  
Vol 87 (12) ◽  
pp. 3723-3727 ◽  
Author(s):  
Nicole Picard-Hagen ◽  
Véronique Gayrard ◽  
Catherine Viguié ◽  
Mohammed Moudjou ◽  
Chantal Imbs ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Physicochemical Properties ◽  
Prion Protein ◽  
Prion Disease ◽  
Genetic Variants ◽  
Proteinase K ◽  
Soluble Form ◽  
C Terminus ◽  
The Mean ◽  
Mean Concentration

The aim of this study was to characterize the cerebrospinal fluid (CSF) prion protein (PrP) of healthy and naturally scrapie-affected sheep. The soluble form of CSF PrPC immunoblotted with an anti-octarepeat and an anti-C terminus mAb showed two isoforms of approximately 33 and 26 kDa, corresponding to the biglycosylated and unglycosylated isoforms of brain PrPC. Neither the mean concentration nor the electrophoretic profile of CSF PrP differed between healthy and scrapie-affected sheep, whereas a slightly increased resistance of CSF PrP to mild proteolysis by proteinase K was evident in the CSF of scrapie-affected sheep. No difference in susceptibility to proteolysis was observed between the two ARR and VRQ genetic variants of the purified prokaryote recombinant PrP. It was concluded that the physicochemical properties of PrPC in the CSF could be altered during scrapie and that these changes might reflect the physiopathological process of prion disease.

scholarly journals BISPHENOL A DETECTION IN PACKAGED DRINKING WATER AND BEVERAGES USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2021 ◽  
Vol 9 (03) ◽  
pp. 111-117
Author(s):  
Neha P. Sangai ◽  
◽  
Himanshu A. Pandya ◽  
Keyword(s):  
Drinking Water ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Bisphenol A ◽  
High Performance ◽  
Bottled Water ◽  
High Concentration ◽  
Bottled Drinking Water ◽  
The Mean ◽  
Mean Concentration

Background: Bisphenol A is characterized as an endocrine disruptor as it interferes with the synthesis of hormones and metabolism resulting in abnormality in the homeostatis of exposed persons. It is used in the production of polycarbonate and epoxy resins which are utilized in the preparation of almost all plastic packaging materials like plastic bottles, cans, food containers, and coating on food containers. Objective: To detect leaching of Bisphenol A in 15 samples of Bottled water and Beverages using High Performance Liquid Chromatography. Methods: Liquid-liquid extraction technique was used for analytical detection of BPA from bottled drinking water and beverages. Results: BPA contamination in Bottled drinking water was calculated through mean concentration for a time period of 30 days as (0.38 ng/ml - 0 day), 8.86 ng/ml (5th day), 17.85 ng/ml (10th day), 30.35 ng/ml (20th day) and 44.48 ng/ml (30th day)). The mean concentration of BPA was observed to be 0.25 to 2.25 ng/ml. Also, the mean concentration of BPA at different temperatures was observed to be 5.96 ng/ml (at 40C), 5.62 ng/ml (at 200C) and 8.80 ng/ml (at 550C). The above results revealed presence of high concentration of BPA in all the samples of bottled drinking water and beverages. Conclusion: The results obtained in the above study depicted considerable amount of BPA leaching from bottled containers into drinking water and beverages. Prolong usage of bottled water and beverages should be avoided to reduce the risk of human exposure to BPA through leaching. Also, it was found that high temperatures resulted in increased BPA leaching.

Methylmalonic Acid Concentration in Serum Not Affected in Hepatic Disease

1992 ◽  
Vol 38 (4) ◽  
pp. 493-495 ◽  
Author(s):  
L Hagelskjaer ◽  
K Rasmussen
Keyword(s):  
Methylmalonic Acid ◽  
Hepatic Disease ◽  
Cobalamin Deficiency ◽  
Metabolic Abnormalities ◽  
Test Results ◽  
High Concentration ◽  
False Positive Test ◽  
Hepatic Diseases ◽  
The Mean ◽  
Mean Concentration

Abstract Accumulation of methylmalonic acid may provide an early clue to deficiency of cobalamin (vitamin B12) in tissue. Metabolic abnormalities involving precursors of methylmalonic acid are frequently observed in patients with hepatic diseases. To establish whether methylmalonic acid accumulates and thereby gives false-positive test results for cobalamin deficiency, we measured the concentration of methylmalonic acid in serum of patients with various hepatic diseases. Many of the patients had increased concentrations of cobalamin in serum. In serum from 70 patients, the mean concentration of methylmalonic acid (252, SE 25 nmol/L) did not differ significantly from that found in healthy subjects (211, SE 12 nmol/L). We conclude that the assay of methylmalonic acid in serum may be useful for evaluating cobalamin status in hepatic disease with functional cobalamin deficiency despite an artificially increased normal or high concentration of cobalamin in serum.

scholarly journals Physicochemical Properties and Metallic Constituent Load in the Water Samples of the Buriganga of Bangladesh

2016 ◽  
Vol 8 (2) ◽  
pp. 141-146
Author(s):  
KM Mohiuddin ◽  
MM Alam ◽  
I Ahmed ◽  
HM Zakir ◽  
AK Chowdhury
Keyword(s):  
Heavy Metal ◽  
Physicochemical Properties ◽  
Water Samples ◽  
Metal Pollution ◽  
Heavy Metal Pollution ◽  
Pollution Prevention ◽  
Aquatic Ecology ◽  
The Mean ◽  
Mean Concentration ◽  
Buriganga River

The study was conducted to assess the level of Cu, Zn, Pb, Cr, Fe, Mn, Cd and Ni contamination in the water samples of the Buriganga river. Total 14 water samples were collected from different areas of upstream of the Buriganga river to determine the physicochemical properties, concentration of different metallic constituents and assess the heavy metal pollution load. Atomic Absorption Spectrometer was used for analyzing the heavy metals of the samples. The mean concentration of Ca, Mg, Na, K in water samples were 0.779, 0.889, 140.39, 26.9 ?g mL-1, respectively. The mean concentration of Cr, Pb, Fe, Cu, Zn and Mn in the water samples were 0.17, 0.05, 0.67, 0.22, 0.55 and 0.17 ?g mL-1, respectively. The amount of Cd in all samples and Pb in 10 samples was below detectable limit of the instrument (0.01 ?g mL-1). Water pH ranged from 4.09 to 7.41 and EC was 346 to 7720 µS cm-1. Magnitude of heavy metal pollution in the Buriganga river system implies that the condition is very alarming and may severely affect the aquatic ecology of the river. To minimize the severe impact on city dwellers and aquatic ecology of the Buriganga river, sustainable steps and continuous monitoring on pollution prevention and cleanup operation is suggested.J. Environ. Sci. & Natural Resources, 8(2): 141-146 2015

LEVELS OF HEAVY METALS IN SOME SELECTED FISH OF RIVER GONGOLA BASIN, ITS DAM AND DADINKOWA DAM, GOMBE STATE NIGERIA

2021 ◽  
Vol 5 (2) ◽  
pp. 498-510
Author(s):  
A. H. Santuraki ◽  
A. U. Babayo ◽  
Abdu Zakari ◽  
A. G. Abdulkadir
Keyword(s):  
Heavy Metals ◽  
Physicochemical Properties ◽  
Suspended Solids ◽  
Anthropogenic Activities ◽  
Clarias Gariepinus ◽  
Dry Season ◽  
Wet Season ◽  
Fish Samples ◽  
The Mean ◽  
Mean Concentration

This study was carried out in ten (10) different locations within River Gongola, its Dam, and Dadinkowa Dam, Gombe State, Nigeria during the wet and dry season to study the water quality in terms of physicochemical properties to ascertain the levels of contaminants due to flooding and anthropogenic activities and the levels of heavy metals (Cr, Cd, Pb, and Ni) in the two species of fish (Clarias gariepinus and Bagrus docmak). A total of 200 water and 360 fish samples comprising two different species were collected in the month of March-June 2017 (Dry season) and July-October 2017 (Wet Season). The results of physicochemical properties obtained ranges between Temperature 29.0-30 0C and 30-32 0C, pH 5.4-7.8 and 6.0-6.9, Conductivity 93.3-161 μS/cm3 and 104.9-128 μS/cm3, Dissolved oxygen 1.2-3.98 mg/l and 0.4-3.1 mg/l, Total Suspended Solids 10-860 mg/l and 13-1180 mg/l, Total Dissolved Solids 46.5-80.5 mg/l and 54.2-76.5 mg/l, Turbidity 424.7-783.5 NTU and 11.15-442.1 NTU, Phosphate 1.82-7.23 mg/l and 0.00-0.11 mg/l, and Nitrate 2.25-8.82 mg/l and 2.68-6.81 mg/l during wet and dry season respectively. The result reveals that turbidity, PO43, DO, TSS, and TDS were above the acceptable permissible limits of WHO While the mean concentration levels of heavy metals in Clarias gariepinus and Bagrus docmak revealed that Cr was below the detectable limit while Cd ranges between 0.5-7.75 µg/g, Pb rangesbetween 3.9-35.6 µg/g during the wet and dry season. The mean concentrations of all the studied metals were above the permissible limit of WHO with a higher concentration during the dry season.

scholarly journals Hydrogen Peroxide and Dicarbonyl Compounds Concentration in Honey Samples from Different Botanical Origins and Altitudes in the South of Saudi Arabia

2019 ◽  
Vol 7 (1) ◽  
pp. 150-160 ◽  
Author(s):  
MOHAMMED ELIMAM AHAMED MOHAMMED ◽  
WED ALARGANI ◽  
MOHAMED A.A. SULEIMAN ◽  
HAMED ALI AL-GRAMAH
Keyword(s):  
Hydrogen Peroxide ◽  
Saudi Arabia ◽  
Sea Level ◽  
Chemical Constituents ◽  
The South ◽  
High Concentration ◽  
Physiochemical Properties ◽  
Gulf Countries ◽  
Low Concentrations ◽  
Acacia Honey

Different factors are known to affect the chemical constituents of the bees honey including the climate and the floral origin. The objective of this study was to investigate the effect of floral origin and altitude on some physiochemical properties of honey samples from the south western part of Saudi Arabia. The investigated physiochemical properties were the pH, conductivity and the concentration of hydrogen peroxide and dicarbonyl molecules. Fifteen Ziziphus spina christi honey samples were collected from three different altitudes in Asir Region (113, 317 and 511 meters above sea level) and twenty fiveAcacia spp honey samples (14, 113, 567, 576 and 2247 meters above sea level). Honey pollens were analyzed to confirm the botanical origin of the honey samples. The pH and conductivity of the honey were determined and compared to three honey standards (Gulf countries standards, CODEX and USA national honey board). The hydrogen peroxide percentage (ww) was determined by titration with ceric sulfate and ferroin as indicator while the dicarbonyls were determined as glyoxal equivalent using spectrophotometer. The pH and conductivity were measured using pH meter and conductometer. The SPSS version 20 program was used for the analysis of the obtained results. The honey samples contained high amounts of the plants pollens and the pH and conductivity were within the standards ranges. The Ziziphus honey was characterized by significantly high mean percentage of hydrogen peroxide (3.9% ± 2.2) compared to the Acacia honey (2.5% ± 0.83) and the Acacia honey contained the highest mean concentration of the dicarbonyl molecules (278.7mg/100g ± 278.4) compared to (147.6mg/ 100g ± 93.2) in the Ziziphus honey. Regarding the effect of altitude, the high altitudes were characterized by low concentrations of hydrogen peroxides and high concentration of dicarbonyl molecules. The altitude significantly increased the dicarbonyl molecules concentration and significantly decreased the hydrogen peroxide percentage.The Ziziphus honey was characterized by significant high percentage of hydrogen peroxide while the Acacia honey was characterized by significant high concentration of dicarbonyl molecules.

scholarly journals Nitric oxide fumigation alleviates chilling injury and regulates fruit quality in sweet orange stored at different cold temperatures

2019 ◽  
pp. 1975-1982
Author(s):  
Muneer Rehman ◽  
Zora Singh ◽  
Tahir Khurshid
Keyword(s):  
Nitric Oxide ◽  
Vitamin C ◽  
Fruit Quality ◽  
Cold Storage ◽  
Storage Temperature ◽  
Chilling Injury ◽  
Injury Incidence ◽  
Total Sugars ◽  
The Mean ◽  
Mean Concentration

The cold storage of sweet oranges below 7 °C causes chilling injury and adversely affects fruit quality. Midknight Valencia and Lane Late sweet oranges were fumigated for 2 hours with different concentrations (5, 10 or 20 µL L-1) of nitric oxide (NO) and stored at (4 or 7 °C) to investigate the effect on chilling injury incidence (CI) and fruit quality after 90 days storage followed by 10 days simulated shelf conditions. Untreated fruit served as a control. The experimental design completely randomised with two factors including NO fumigation treatments and storage temperatures. All NO fumigation treatments (5, 10 or 20 µL L-1) significantly reduced the CI irrespective of storage temperature as compared to the control in both the cultivars. Fruit were fumigated with different concentration of NO gas in a sealed container for 2 h and then kept at 4°C and 7°C. Fruit quality variables such as fruit firmness, SSC (%), TA (%), SSC/TA, sugars, vitamin C and total antioxidants were determined. All the NO treatments significantly reduced per cent weight loss as compared to control in Lane Late. Mean weight losses were higher (8.3 % and 5.5 %) when fruit were stored at 7 °C as compared to those stored at 4 °C (4.8 % and 3.5 %) in Midknight Valencia and Lane Late respectively. All the NO fumigation treatments significantly reduced the mean concentrations of glucose, fructose, sucrose and total sugars in the juice of Midknight Valencia only. All NO fumigation treatments significantly reduced mean concentration vitamin C in the fruit juice of Lane Late as compared to the control. Meanwhile, in Midknight Valencia, NO (10 or 20 µL L-1) fumigated fruit showed a significant reduction in the mean concentration of vitamin C as compared to NO (5 µL L-1) fumigation and control. The juice of Midknight Valencia had higher mean total antioxidants when fumigated with NO (5 µL L-1) as compared to the control, but not in Lane Late. In conclusion, all the NO fumigation (10 µL L-1) treatment was most effective in reducing CIin both cultivars irrespective of the cold storage temperature. NO fumigation treatments did not affect SCC/TA ratio but reduced all the individual and total sugars as well as vitamin C in the fruit stored for 90 days followed by 10 days simulated shelf conditions.

Antioxidant Vitamins in Honey Samples from Different Floral Origins and Altitudes in Asir Region at the South-Western Part of Saudi Arabia

2019 ◽  
Vol 15 (3) ◽  
pp. 296-304 ◽  
Author(s):  
Amal Al-Mosa ◽  
Eid I. Brima ◽  
Khaled F. Fawy ◽  
Hamed A. AL Ghrama ◽  
Mohammed E.A. Mohammed
Keyword(s):  
Vitamin A ◽  
Vitamin C ◽  
Mean Value ◽  
The Other ◽  
P Value ◽  
Redox Titration ◽  
Physiochemical Properties ◽  
Acacia Honey ◽  
The Mean ◽  
Asir Region

Background: Different factors are known to affect the different physiochemical properties of bee's honey including the floral origin and climate. Objective: The aim of this article was to investigate the effect of floral origin and altitude on the concentration of vitamin C and A. Methods: Ten Ziziphus honey samples were collected from two altitudes; 113 and 511 meters. Twenty-five Acacia honey samples were included in this study from five altitudes; 14, 113, 317, 576 and 2247 meters above sea level. Vitamin C was measured by redox titration and vitamin A was estimated using the Carr-price reaction and spectrophotometer. Results: The mean± SD of vitamin C in the Ziziphus and Acacia honey samples were 239.2± 91.5 and 260.4± 81.1 mg\100g, respectively. Vitamin A mean value± SD in the Ziziphus and Acacia honey were 0.088± 0.126 and 0.062± 0.126 mg\ 100g, respectively. Concerning the effect of altitude, there was significant increase in vitamin C concentration in ziziphus honey with the increase of the altitude (p- value= 0.027), while an insignificant decrease was obtained in the case of vitamin A. Regarding the effect of altitude on the concentration of vitamin C in the Acacia honey, there was significant increase in the honey from the altitude of 2247 compared to the honey from all the other altitudes. Vitamin A concentration in the Acacia honey from the altitude 2247 was insignificantly decreased compared to the honey from all the other altitudes (p- value > 0.05). Conclusion: The floral origin insignificantly affected the concentration of vitamin C and A. The altitude significantly affected the concentration of vitamin C irrespective of the floral origin.

scholarly journals The assessment of hepatic mineral composition in sheep, cattle, chicken, and fish in Erbil City, Kurdistan Region-Iraq

2021 ◽  
pp. 46-55
Author(s):  
Abdulqader Ahmed Hussein
Keyword(s):  
Dry Weight ◽  
High Concentration ◽  
Toxic Level ◽  
Mean Values ◽  
X Ray ◽  
The Mean ◽  
Mineral Concentrations ◽  
Fluorescence Spectrometer ◽  
The City ◽  
Mean Concentration

Liver mineral concentrations of 232 samples from slaughtered animals, including  lamb, yearling, sheep, and cattle, checkin, and fish were detrmined, using X-ray fluorescence spectrometer, to provide data regarding to the liver mineral concentrations (mg/kg dry weight). The mean liver Zn and Co liver conentrations of lambs (286, 1.9), yearling (233, 1.7), sheep (254, 1.8), and cattle (276, 1.8) in majority samples were adequate, while liver Pb concentrations of mentioned animals (45.3, 48.1, 46.4, 39.6) mg/kg respectively were high and exceeded toxic levels. In checkin, the mean liver Cu, Zn, and Co were also adequate, with the mean values of (26.4), (298.8), and (1.8) mg/kg respectively. Though, the mean liver Fe concentration (2257.0 mg/kg) was high, and liver Pb concentration (6.2 mg/kg) was reched toxic level. In fish, for majority liver samples, the mean concentration of Cu (191.8), Fe (6800.0), Zn (202.8), Co (3.3), Pb (8.6), and Ni (6.3) were high and toxic. In conclusion, liver Pb (and Ni only in fish) concentration in all studied animals for the majority of examined sample were higher than normal and toxic. Wherese, other studied minerals in majority of liver samples were adequate. Therefore, it can be concluded that there are problems on animal and human health (due to high concentration of Pb and Ni) would be raised at present from the consumption of ovine, bovine, checkin, and fish slaughtered from the city of Erbil.

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