scholarly journals The Role of Volume, Conductivity, Scatter Changes of Neutrophils and Monocytes in Diagnosis of Megaloblastic Anemia

2020 ◽  
Vol 7 (12) ◽  
pp. A563-569
Author(s):  
Deepa Sowkur Anandarama Adiga ◽  
Debarshi Saha ◽  
Karthick R G ◽  
Vishnu Priya M ◽  
Purnima S Rao ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Megaloblastic Anemia ◽  
Serum Vitamin ◽  
Volume Conductivity ◽  
Degree Of Certainty ◽  
Normochromic Anemia ◽  
The Mean ◽  
The Difference ◽  
And Control

Background: Megaloblastic anemias are macrocytic normochromic anemia with mean corpuscular volume (MCV) of 100 fl-140 fl and caused by deficiency of either cobalamin (vitamin B12) or folate. However, increased MCV is not specific for megaloblastic anemia, nor is Vitamin B12 assay by chemiluminescence. We undertook this study to evaluate the possible role of Volume, Conductivity and Scatter (VCS) of WBCs derived from standard hematology analyzer to indicate megaloblastic anemia. Methods: We performed a case control study comparing data of 60 patients with low serum vitamin B12 or folate levels with 60 healthy volunteers. Comparison of the volume, conductivity and scatter parameters for neutrophils and monocytes of cases and control were done. Result: The mean neutrophil volume of cases (158.37±18.13fl) was significantly higher (p= 0.0001) compared to controls (141.26±4.22fl). Similarly, mean monocyte volume of cases (183.34±16.90fl) was significantly (p=0.0001) higher compared to controls (166.55±8.66fl). The difference in the mean conductivity of both neutrophils and monocytes between cases and controls were insignificant (p=0.43). Conclusion: Our study suggests analysis of VCS parameters for neutrophils and monocytes was a simple and objective method that substantiates the existence of subclinical deficiency of vitamin B 12 and folate with fair degree of certainty.

scholarly journals The Relation between Iron and Vitamin B12 Status with Coronary Artery Disease

2021 ◽  
pp. 105-115
Author(s):  
Kholoud M. Shamallakh ◽  
Ohood M. Shamallakh ◽  
Heba M. Arafat ◽  
Mazen M. Alzaharna
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Vitamin B12 ◽  
Serum Iron ◽  
Transferrin Saturation ◽  
Serum Vitamin ◽  
The Mean ◽  
The Difference ◽  
Artery Disease ◽  
Control Study

Aims: To investigate the association of iron and vitamin B12 status with coronary artery disease in Gaza city. Study Design: A Case control study Place and Duration of Study: Samples were collected from the cardiac unit at Al-Shifa hospital, Gaza Strip. Methodology: Case-control study was conducted on a sample of 31 patients with coronary artery disease (CAD) and 27 apparently healthy controls aged between 30-60 years. Interviewed questionnaire was conducted among the study population. Vitamin B12, serum ferritin, serum iron, lipid profile parameters, and high sensitivity C reactive protein were performed. An approval was acquired from Helsinki ethical committee to perform this study. All data were analyzed by SPSS. Results: The results showed that the mean level of serum iron in cases (71.6 ± 24.7 µg/dl) was lower compared to that of controls (87.3 ± 28.4 µg/dl) and the difference was statistically significant (P=.028). Moreover, transferrin saturation percentage in cases (24.0 ± 8.9%) was lower compared to controls (29.0 ± 9.9%) and the difference was statistically significant (P=.045). In addition, the mean levels of serum vitamin B12 in cases (238.8 ± 51.4 pg‎/dl) was lower compared to controls (337.3± 108.4 pg‎/dl) and was statistically significant (P<0.001). The Pearson correlation test showed that there was a significant positive correlation between the level of serum iron with the level of vitamin B12 among the participants (r = 0.28, P=.032). Conclusion: The mean differences of transferrin saturation, serum ferritin, and vitamin B12 between cases and controls were statistically significant. The mean levels of serum vitamin B12 in cases was lower compared to controls and was statistically significant.

Serum Vitamin B12 and Folic Acid Levels in Hyperthyroidism

1979 ◽  
Vol 18 (06) ◽  
pp. 278-282 ◽  
Author(s):  
Matina Kesse-Elias ◽  
D. Koutras ◽  
P. Pandos ◽  
S. Papazoglou ◽  
S. Moulopoulos ◽  
...  
Keyword(s):  
Folic Acid ◽  
Vitamin B12 ◽  
Serum Vitamin ◽  
Mean Value ◽  
Clinical Index ◽  
The Mean ◽  
The Difference ◽  
Serum Folic Acid ◽  
Hyperthyroid Patients ◽  
Normal Controls

SummarySerum vitamin B12 and folic acid levels were measured in 48 hyperthyroid patients and in a group of euthyroid controls. The levels of vitamin B12 ranged from 120-900 pg/ml with a mean of 429.3 ± 30.9 pg/ml (SE). The mean serum vitamin B ? level was lower in hyperthyroid patients than in normal controls, the difference being statistically significant (t = 2.584, p < 0.025). Serum vitamin B12 levels showed a statistically significant negative correlation with the clinical index of Grooks et al. (r = 0.344, p < 0.05). The findings, although not excluding the involvement of auto-immune gastritis in patients with low serum vitamin B12 levels, suggest a direct action of increased thyroid hormone concentrations. Serum folic acid levels ranged from 0.5- 13.8 ng/ml with a mean of 6.8 ± 0.46 ng/ml (SE). The mean serum folic acid levels were higher in the hyperthyroid patients than in normal controls but the difference was not statistically significant (t = 1.2, p > 0.2). The serum folic acid levels did not show any statistically significant correlation with the clinical index of Grooks et al. The fact that no statistically significant difference was found between the mean value in hyperthyroid patients and the mean value in normal controls is probably due to the high folic acid intake in Greece.

scholarly journals Relationship Between Serum Vitamin D Levels and Serous Otitis Media in Children with Adenoid Hypertrophy Based on Age

Thrita ◽  
2020 ◽  
Vol 8 (2) ◽  
Author(s):  
Maryam Biglari Abhari ◽  
Hossein Majidinia ◽  
Farahnaz Beyranvand ◽  
Arash Tehrani Banihashemi ◽  
Fatemeh Dehghani Firouzabadi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Otitis Media ◽  
Serum Vitamin ◽  
Adenoid Hypertrophy ◽  
Serum Vitamin D ◽  
Vitamin D Levels ◽  
The Mean ◽  
The Difference ◽  
Serous Otitis Media

Background: Serous Otitis Media (SOM) is one of the most common diseases of children that can lead to hearing loss and imbalance. Recently, the role of vitamin D has been identified in strengthening and regulating the immune system. Moreover, vitamin D supplementation has been recognized to improve the immune response against upper respiratory infection and pathogen overgrowth. Methods: A cross-sectional study was performed on 89 children aged 1 - 15 years with adenoid hypertrophy who met the inclusion criteria. The serum vitamin D level was compared between the two groups of children with and without SOM. Results: The mean age of the target children was 69.57 ± 25.65 months. The mean serum vitamin D level was 23.86 ± 12.7 in all patients. The mean serum vitamin D level was 24.7 ± 13.1 and 22.9 ± 12.2 in patients with and without SOM (based on tympanometry), respectively, yet the difference was not statistically significant. However, the difference was significant between the two groups of children with the age of < 84 months and > 84 months (P = 0.05). Conclusions: The role of vitamin D was identified in increasing the incidence of SOM in children older than 84 months with adenoid hypertrophy. In older children, the timely diagnosis and treatment of vitamin D deficiency can prevent severe SOM complications, which can save a lot of costs for families and the health system.

Seasonal trends in the stable isotopic composition of snow and meltwater runoff in a subarctic catchment at Okstindan, Norway

2004 ◽  
Vol 35 (2) ◽  
pp. 119-137 ◽  
Author(s):  
S.D. Gurney ◽  
D.S.L. Lawrence
Keyword(s):  
Isotopic Composition ◽  
Mean Value ◽  
Mean Values ◽  
Stable Isotopic Composition ◽  
Meltwater Runoff ◽  
Stable Isotopic ◽  
The Mean ◽  
The Difference ◽  
Δ18o And Δd

Seasonal variations in the stable isotopic composition of snow and meltwater were investigated in a sub-arctic, mountainous, but non-glacial, catchment at Okstindan in northern Norway based on analyses of δ18O and δD. Samples were collected during four field periods (August 1998; April 1999; June 1999 and August 1999) at three sites lying on an altitudinal transect (740–970 m a.s.l.). Snowpack data display an increase in the mean values of δ18O (increasing from a mean value of −13.51 to −11.49‰ between April and August), as well as a decrease in variability through the melt period. Comparison with a regional meteoric water line indicates that the slope of the δ18O–δD line for the snowpacks decreases over the same period, dropping from 7.49 to approximately 6.2.This change points to the role of evaporation in snowpack ablation and is confirmed by the vertical profile of deuterium excess. Snowpack seepage data, although limited, also suggest reduced values of δD, as might be associated with local evaporation during meltwater generation. In general, meltwaters were depleted in δ18O relative to the source snowpack at the peak of the melt (June), but later in the year (August) the difference between the two was not statistically significant. The diurnal pattern of isotopic composition indicates that the most depleted meltwaters coincide with the peak in temperature and, hence, meltwater production.

The effect of antibiotic dietary supplements on the carcass measurements and dressing percentage of bacon pigs

1955 ◽  
Vol 46 (2) ◽  
pp. 173-179 ◽  
Author(s):  
G. Harrington ◽  
J. H. Taylor
Keyword(s):  
Growth Rate ◽  
Vitamin B12 ◽  
Dietary Supplements ◽  
Vegetable Protein ◽  
Animal Protein ◽  
Feeding Trials ◽  
The Mean ◽  
Dressing Percentage ◽  
And Control

1. Certain carcass measurements (length, shoulder and mid-back fats, belly thickness and dressing percentage) made on a total of 402 bacon pigs from four antibiotic feeding trials have been analysed.2. The length of the carcasses showed more variation between pens treated with various doses of penicillin than between control pens or pens treated with aureomycin. This greater variation in length was related to the greater variation in growth rate between the penicillin treatments. No differences in the mean lengths of control, penicillin and aureomycin treatments were found.3. Back fat measurements and belly thickness showed no differences between aureomycin, penicillin and control treatments in overall tests.4. Aureomycin consistently gave higher dressing percentages than penicillin. Animal protein also gave higher dressing percentages than vegetable protein, but no effect of vitamin B12 on this quantity was found.

scholarly journals True and Pseudo Cholinesterase levels in short and long-term of pesticides exposures

2020 ◽  
pp. 9-12
Author(s):  
Mohammed Abdul Rahman ◽  
Raghunatha Rao D ◽  
Vasantha L
Keyword(s):  
Whole Blood ◽  
Acute Poisoning ◽  
Mean Value ◽  
Serum Cholinesterase ◽  
Control Group ◽  
Study Group ◽  
Chronic Poisoning ◽  
The Mean ◽  
The Difference ◽  
And Control

The present study is aimed to study and analyze the true and pseudo Cholinesterase levels in the subjects exposed to pesticides during short term by intentional or accidental intake and long term exposure due to their occupation were studied at Department of Biochemistry, SVS medical college and hospital mahbubnagar district. Whole blood cholinesterase levels and Pseudo cholinesterase levels were estimated. 150 people were taken as controls who had no medical illness and a total number of 300 cases of pesticide poisoning were selected, aged between 20 to 55 years, which consist of 150 acute poisoning and 150 chronic poisoning subjects in Mahbubnagar district, were taken as case study, the detailed case history and the type of organophosphorus pesticide taken were recorded. Mean and standard deviation (S.D) of all variables were calculated and compared with those of controls. Statistical significance was assessed and P-value <0.05 were considered significant.           During acute poisoning the mean value of Whole blood cholinesterase/True cholinesterase (U / L) in acute poisoning cases on first day was 1.267± 0.612 on 3th day was 1.651±0.647, on 7th day was 2.221±0.684 and at the end of 6 months was 3.970±0.404.The difference between the study group and control group (4.0 ± 0.39) was found to be statistically significant in1st, 3rd, 7th day but not significant at the end of 6 months. The mean value of serum cholinesterase/pseudo cholinesterase (U/L) in acute poisoning cases on first day was 2213.05 ± 1749.81, on 3th day was 2862.3 ± 2025.6, on 7th day was 4008.4 ± 2355.9 and at the end of 6 months was 7708.34 ± 880.72.The difference between the study group and control group (7991.97 ± 1276.5) was found to be statistically significant in1st, 3rd, 7th day but not significant at the end of 6 months. During chronic poisoning (exposure) the mean value of Whole blood cholinesterase (U/L) in controls is 4.0 ± 0.39 as compared to 3.019 ± 0.848 in cases of chronic poisoning. The difference between the study group and control group was found to be statistically significant. The mean value of serum cholinesterase/pseudo cholinesterase (U/L) in controls was 7991.97 ± 1276.5 as compared to 6214 ± 1189 in cases of chronic poisoning. The difference between the study group and control group was found to be statistically significant.

scholarly journals Circulating antibody to transcobalamin II causing retention of vitamin B12 in the blood

Blood ◽  
1977 ◽  
Vol 49 (6) ◽  
pp. 987-1000 ◽  
Author(s):  
R Carmel ◽  
B Tatsis ◽  
L Baril
Keyword(s):  
Vitamin B12 ◽  
Binding Capacity ◽  
Megaloblastic Anemia ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
High Serum ◽  
The Status ◽  
B12 Deficiency ◽  
Long Acting

A patient with recurrent pulmonary abscess, weight loss, and alcoholism was found to have extremely high serum vitamin B12 and unsaturated vitamin B12-binding capacity (UBBC) levels. While transcobalamin (TC) II was also increased, most of his UBBC was due to an abnormal binding protein which carried greater than 80% of the endogenous vitamin B12 and was not found in his saliva, granulocytes, or urine. This protein was shown to be a complex of TC II and a circulating immunoglobulin (IgGkappa and IgGlambda). Each IgG molecule appeared to bind two TC II molecules. The reacting site did not interfere with the ability of TC II to bind vitamin B12, but did interfere with its ability to transfer the vitamin to cells in vitro. The site was not identical to that reacting with anti-human TC II antibody produced in rabbits. Because of this abnormal complex, 57Co-vitamin B12 injected intravenously was cleared slowly by the patient. However, no metabolic evidence for vitamin B12 deficiency was demonstrable, although the patient initially had megaloblastic anemia apparently due to folate deficiency. The course of the vitamin B12-binding abnormalities was followed over 4 yr and appeared to fluctuate with the status of the patient's illness. The IgG-TC II complex resembled one induced in some patients with pernicious anemia by intensive treatment with long-acting vitamin B12 preparations. The mechanism of induction of the antibody formation in our patient is unknown.

scholarly journals EFEK FORTIFIKASI VITAMIN B12 TERHADAP KADAR VITAMIN B12 SERUM DAN HOMOSISTEIN SERUM PADA VEGETARIAN

2020 ◽  
Vol 11 (1) ◽  
pp. 114-120
Author(s):  
Susianto Susianto
Keyword(s):  
Vitamin B12 ◽  
Homocysteine Level ◽  
Megaloblastic Anemia ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Natural Sources ◽  
Serum Homocysteine ◽  
Microparticle Enzyme Immunoassay ◽  
B12 Deficiency ◽  
Community Trial

Introduction: Vegetarians consume plant-based foods with or without eggs and milk. Vegetarians are at risk of vitamin B12 deficiency, as natural sources of vitamin B12 are limited to animal-based foods. Vitamin B12 deficiency can lead to megaloblastic anemia, nerve damage and increase homocysteine level. Higher homocysteine level can increase the risk of coronary heart disease and stroke. The objective of this study was to investigate the effect of vitamin B12 fortification on the level of serum vitamin B12 and homocysteine in vegetarian. Method: The research design was an experimental study, community trial. The samples were 42 vegetarians with vitamin B12 deficiency (< 156 pmol/L) selected from 118 vegetarians as members of Indonesia Vegetarian Society (IVS) Pekanbaru, treated by vitamin B12 fortified oatmeal for three months from March to June 2010.  Serum vitamin B12 and homocysteine were measured by electrochemiluminescent immunoassay and microparticle enzyme immunoassay method respectively. Result: Prevalence of vitamin B12 deficiency in vegetarian was 35.6%. Statistical analysis showed a significant increase of serum vitamin B12 from 124.6 to 284.6 pmol/L (p=0.001) and significant decrease of serum homocysteine from 20.1 to 15.1 µmol/L (p=0.001). Conclusion: Consumption of vitamin B12 fortified oatmeal increases the level of serum vitamin B12 and decreases the level of serum homocysteine significantly in vegetarian with vitamin B12 deficiency.

DECREASED PROSTACYCLIN-LIKE ACTIVITY IN HENOCH-SCHONBEIN PURPURA

1987 ◽  
Author(s):  
J J F Belch ◽  
S Turi ◽  
M MacLaren ◽  
T J Beattie ◽  
C D Forbes
Keyword(s):  
Platelet Rich Plasma ◽  
Skin Lesions ◽  
Endothelial Damage ◽  
Common Type ◽  
Intestinal Involvement ◽  
Childhood Vasculitis ◽  
The Difference ◽  
Umbilical Arteries ◽  
And Control

Henoch-Schoniein purpura (HSP) is the most common type of childhood vasculitis. The skin lesions are the most obvious sign but visceral involvement carries a more serious prognosis. Increasing amounts of data are available regarding the possible role of prostacyclin (PGI2) in the pathogenesis of other vasculo-pathies, but no-one has previously investigated HSP. 17 HSP patients (mean age (SD) 5.7 (2.5) years) and 17 matched controls were studied. The ability of plasma (patient and control) to support PGI2 -like activity was tested. Human umbilical arteries were chopped into rings and incubated in buffer at 37° C. Aliquots of the supernatant were added to platelet rich plasma (PRP) and platelet aggregation measured (Malin's aggregometer). Repeated washings of the rings then depleted PGI. production. The rings were then incubated with test platelet poor plasma (PPP) at 37° C. PGI2 like activity was assessed as before and the results expressed as a percentage of the aggregation obtained by the same exhausted ring before addition of PPP. The results show that the ability of test PPP to support PGI2 like activity was reduced in 13/17 HSP patients. The difference between patients (21.3 (SD 20.5%) and controls (57.2 (SD 12.2%) was significant (p<0.001). Patients with renal and gastro-intestinal involvement (7.5 (SD 18.1%) were significantly different from those patients with only skin involvement (32.3 (SD 23.5%) (p<0.01). Plasma from six patients in which PGI^like activity was undetectable using the above method were further studied to detect PGI2 -inhibitory activity. Unexhausted umbilical rings were incubated at 37°C for 5mins. The baseline PGI2 like activity of the supernatant was compared with that produced after 5mins. incubation with test PPP (o patients, 6 controls). Less like activity was produced by the unexhausted rings when incubated with PPP from the HSP patients than with either control buffer or control PPP (both p<0.01). It seems likely that these abnormalities, having been demonstrated in yet another vasculitic syndrome, are secondary to endothelial damage. The disturbances, however, may well be of importance in extending the primary microvascular insult

Ototoxicity of Topical Ciprofloxacin/Dexamethasone otic Suspension in a Chinchilla Animal Model

2008 ◽  
Vol 139 (6) ◽  
pp. 840-845 ◽  
Author(s):  
Sam J. Daniel ◽  
Raymundo Munguia
Keyword(s):  
Tube Insertion ◽  
Auditory Brain Stem Response ◽  
Normal Morphology ◽  
Auditory Brain Stem ◽  
Ventilation Tubes ◽  
The Mean ◽  
Contralateral Ear ◽  
The Difference ◽  
And Control ◽  
To Receive

Objective To determine the ototoxic potential of ciprofloxacin/dexamethasone eardrops. Methods Ventilation tubes were inserted in the ears of 15 chinchillas. One ear was randomized to receive four drops of ciprofloxacin/dexamethasone. A control solution (0.45% NaCl) was applied in the contralateral ear. The eardrops were delivered twice daily for 7 consecutive days. Auditory brain stem response (ABR) measurements were recorded at baseline (post-tympanostomy tube insertion), and on days 4, 8, 15, 22, and 60. Results An initial transient increase of 8.11 dB nHL was noted in the mean thresholds of the experimental ears at day 4. On the last ABR evaluation (day 60), the mean thresholds were 16.33 ± 6.93 dB nHL in the experimental ears and 13.66 ± 6.65 dB nHL in the control ears. The difference in the mean thresholds between the experimental and control ears at day 60 was not significant (95% confidence interval, 3.12, –8.02). Scanning electron microscopy demonstrated normal morphology in all experimental ears that were assessed. Conclusion Ciprofloxacin/dexamethasone eardrops did not appear ototoxic when used in chinchillas post-ventilation tube insertion.

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