scholarly journals Immune status of children with complications of BCG/BCG-M vaccination

2020 ◽  
Vol 98 (1) ◽  
pp. 27-34
Author(s):  
T. A. Sevostyanova ◽  
V. A. Аksenova ◽  
D. A. Kudlay
Keyword(s):  
Serum Level ◽  
Normal Level ◽  
Elevated Level ◽  
Pediatric Patients ◽  
Immune Status ◽  
Phagocytic Index ◽  
Lymphocyte Population ◽  
Flow Cytofluorometry ◽  
Low Level ◽  
Igg Level

The objective of the study: to analyze certain parameters of immune status in children with complications after BCG/BCG-M vaccination.Subjects and methods. The immune status parameters were tested in 24 children who developed complications after TB vaccination such as BCG-ostitis, BCG-lymphadenitis. Lymphocyte population was assessed using flow cytofluorometry by Cytomics FC 500 (Bekman Coulter).The serum level of immunoglobulins IgG, IgA, IgM was tested by immunodiffusion in Mancini agar. The leukocyte phagocytic activity was tested using flow cytofluorometry (Cytofluorimeter Cytomics FC 500) with FITC-labeled staphylococcus.Results. Of the 24 children with BCG lymphadenitis or BCG ostitis, 22 (92%; 95% CI 73-99%) had abnormality in immune status.Of the 24 children, 7 had low CD3+ count, while 9 had an elevated level of CD3+ . The level of СD4+ was low in 4 children and elevated in 15 pediatric patients. CD8+ count was low in 21 children (88%; 95% CI 72-97%). 11 children had low СD16+ number, and it was elevated in 2 children. In 10 children, low level of СD16+ was combined with low level of CD8+ . 10 children had low СD19+ number, and it was elevated in 4 children. The leukocyte phagocytic index drastically decreased (down to 37%) only in 1 child, and in 23 children, it varied from 60 to 90%.IgG level was low in 13 (54%), and elevated in 2 (8%) children. IgA level was low in 6 (25%), and it was not detected at all in 8 (33.3%) children. IgM level was elevated in 4/24 (17%) children, while it was low in 1/24 (4%). In 8 children with no IgA detected, the IgG level was low in 5 of them, 3 had a normal level, and IgM level was elevated in 2 children, 6 children had a normal level of IgM. With IgA completely absent, 1 child had the normal level of IgG and IgM.

Some Cytokines Levels (IL-6 and IL-10) in Sera Patients with Cutaneous Leismanasis in Nassiriya Province

2020 ◽  
pp. 4-6
Keyword(s):  
Serum Level ◽  
Immune Status ◽  
Pure Science ◽  
Control Subjects ◽  
Significant Difference ◽  
Healthy Control

The present study was carried out in the Labs of collage of education for pure science, during period from January 2017 to endDecember of the same year. The immune status investigates for CLpatients by measuring the levels of cytokines (IL6and IL10) in sera using a technique enzyme-linked immune Sorbent adsorptive (ELISA). The study included 120 subjects with (60 CLpatientsL.majar and 60 CLpatients L. tropica with and (30) were healthy control. Increased mean Serum level of IL6 was in the observed in the total patients as compared to control Subjects (224.53pg/ml,70.70pg/ml), the result indicate there was significant difference at (p<0.05) ,such observation was consistent in the patient infected with L.majar and L. tropica (104 .90 pg/ml and 112.78 pg/ml) respectively. The results of the IL10 showed significant difference at (p<0.05)increased of mean Serum level in the total CL patients as compared to control Subjects(226.90 pg/ml 46.77pg/ml,).Ahighly significant difference at (p<0.05) increased observed in patients group infected with L.majar and followed by patients group infected L. tropica (112.78pg/ml and 114.12pg/ml) respectively.These results revealed that the excessive presence of cytokines might play a role in CL patients.

Image retrieval of MRI brain tumour images based on SVM and FCM approaches

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Sonia Bansal ◽  
Vineet Mehan
Keyword(s):  
Image Retrieval ◽  
Medical Image ◽  
Elevated Level ◽  
Critical Level ◽  
Support Vector ◽  
Resonance Imaging ◽  
Power Structures ◽  
Low Level ◽  
Mri Brain ◽  
Level Data

Abstract Objectives The key test in Content-Based Medical Image Retrieval (CBMIR) frameworks for MRI (Magnetic Resonance Imaging) pictures is the semantic hole between the low-level visual data caught by the MRI machine and the elevated level data seen by the human evaluator. Methods The conventional component extraction strategies centre just on low-level or significant level highlights and utilize some handmade highlights to diminish this hole. It is important to plan an element extraction structure to diminish this hole without utilizing handmade highlights by encoding/consolidating low-level and elevated level highlights. The Fleecy gathering is another packing technique, which is applied in plan depiction here and SVM (Support Vector Machine) is applied. Remembering the predefinition of bunching amount and enlistment cross-section is until now a significant theme, a new predefinition advance is extended in this paper, in like manner, and another CBMIR procedure is suggested and endorsed. It is essential to design a part extraction framework to diminish this opening without using painstakingly gathered features by encoding/joining low-level and critical level features. Results SVM and FCM (Fuzzy C Means) are applied to the power structures. Consequently, the incorporate vector contains all the objectives of the image. Recuperation of the image relies upon the detachment among request and database pictures called closeness measure. Conclusions Tests are performed on the 200 Image Database. Finally, exploratory results are evaluated by the audit and precision.

In Reply: Pathogenesis of Hyposthenuria in Sickle Cell Anemia

PEDIATRICS ◽  
1960 ◽  
Vol 26 (6) ◽  
pp. 1051-1052
Author(s):  
Hans G. Keitel
Keyword(s):  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Normal Level ◽  
Urinary Concentration ◽  
Urea Excretion ◽  
Low Level ◽  
Concentration Of Urine

In reply to the question raised by Dr. Morgan, it is now well established that urea plays an important part in the concentration of urine. Starting at a very low level of urea excretion, the urinary concentration maxima (as obtained during prolonged fluid deprivation, or following sustained vasopressin stimulation) is less than normal. When the rate of urea excretion is increased to the normal level, the maximal urinary concentration ability increases, but when the rate of urea excreted increases markedly, the urinary concentration maxima once again decreases.

Studies of Blood Lipid Fractions of Quiescent Rheumatic Fever Patients and Their Siblings

1959 ◽  
Vol 5 (6) ◽  
pp. 557-565 ◽  
Author(s):  
Harold D Appleton ◽  
Alvin F Coburn ◽  
Yale L Meltzer
Keyword(s):  
Rheumatic Fever ◽  
Normal Level ◽  
Plasma Levels ◽  
Blood Lipids ◽  
Lipid Fraction ◽  
Blood Lipid ◽  
Low Level ◽  
Lipid Fractions ◽  
The Difference

Abstract Three phospholipid fractions have been determined in plasmas of quiescent rheumatic children and their siblings of similar ages. The method for determining blood lecithin gave reproducible results, and the lipid fraction appeared indicative of difference in levels of blood lipids between rheumatic and nonrheumatic children. In this series of lecithin determinations only one rheumatic subject had a high normal level and only one sibling (presumably nonrheumatic) had a low level. The difference in mean nonfasting lecithin plasma levels between rheumatic subjects and siblings was 0.36 mMol./L. Similarly, the lecithin level of each of the nonrheumatic siblings in this series averaged 0.41 mMol./L. higher than that of the corresponding quiescent rheumatic child. In both of these differences P &lt;0.05.

scholarly journals Low Level of Vitamin D is Correlated with High C-Reactive Protein (CRP) and Disease Activity in Indonesian Juvenile Idiopathic Arthritis (JIA) Patients

2020 ◽  
Vol 12 (2) ◽  
pp. 149-56
Author(s):  
Desy Wulandari ◽  
Wisnu Barlianto ◽  
Tita Luthfia Sari
Keyword(s):  
Vitamin D ◽  
Juvenile Idiopathic Arthritis ◽  
Serum Level ◽  
Disease Activity ◽  
Vitamin D Deficiency ◽  
Control Group ◽  
Healthy Children ◽  
C Reactive Protein ◽  
Low Level ◽  
Reactive Protein

BACKGROUND: Vitamin D plays essential role in the regulation of inflammation, such as in pathogenesis of Juvenile Idiopathic Arthritis (JIA). Vitamin D deficiency has been reported among JIA patients, but there were conflicting results regarding the correlation with disease activity. This study aimed to assess vitamin D serum level and its correlation with C-Reactive Protein (CRP) and disease activity in JIA patients.METHODS: Children who were diagnosed with JIA according to International League of Associations for Rheumatology (ILAR) criterias were enrolled as JIA group subjects, while age and sex-matched healthy children were enrolled as the control group subjects. Vitamin D and CRP serum level were measured. Disease activity of JIA patients was calculated by Juvenile Arthritis Disease ActivityScore-27 (JADAS-27).RESULTS: Vitamin D serum level was lower in the JIA group compared to the healthy control group (p=0.000). Among 26 JIA patients, 61.5% were deficient, 30.8% were insufficient, and 7.7% had normal vitamin D. No significant different in CRP level between vitamin D group (p=0.441), but there was significant different in JADAS-27 (p=0.001). The mean of CRP and JADAS-27 were found highest in vitamin D deficiency group. Vitamin D serum level was negatively correlate with CRP (p=0.021, r=-0.452) and JADAS-27 (p=0.001 r=-0.595).CONCLUSION: Low level of vitamin D in JIA patients was inversely related to higher CRP and disease activity,suggesting that vitamin D supplementation could be havepotential role in JIA treatment.KEYWORDS: vitamin D, CRP, disease activity,JADAS-27, JIA

scholarly journals Features of the immune status in patients with chronic and complicated pyodermas: choice of a therapeutic correction method

2021 ◽  
Vol 7 (2) ◽  
pp. 15-21
Author(s):  
Roman A. Khmel’nitskij ◽  
Andrej V. Vislobokov ◽  
Pavel V. Kalutskii
Keyword(s):  
Proinflammatory Cytokines ◽  
Transfer Factor ◽  
Immune Status ◽  
Correction Method ◽  
Phagocytic Index ◽  
Immunological Parameters ◽  
Transfer Factors ◽  
Drug Of Choice ◽  
Before And After ◽  
Antimicrobial Protection

Introduction: To compare the features of the immune status in patients with chronic and complicated pyoderma in the course of complex pharmacotherapy using immunomodulators based on transfer factors and glucosaminylmuramyldipeptide. Materials and methods: A clinical examination of 107 patients with pyoderma, divided into three groups, was carried out. All individuals underwent immunological examination before and after etiopathogenetic treatment. The patients of the first group were additionally treated with a drug containing signaling immunoactive molecules (transfer factor) as an immunomodulator; the patients of the second group received glucosaminylmuramyldipeptide; and the patients of the third group received standard antibacterial therapy. Results and discussion: Prior to the beginning of pathogenetic therapy, the patients were found to lack non-specific mechanisms of antimicrobial protection; there was a decrease in the activity and intensity of phagocytosis: phagocytic index and phagocytic number of neutrophils by 1.2 and 1.3 times; the production of proinflammatory cytokines IL-6 increased by 2.3 times, IL-8 –by 2.1 times, and TNFa – by 2.4 times. The study of immunological parameters after the inclusion of immunomodulators into the therapy revealed an increase in the phagocytic activity of neutrophils, and the indicators of the NST test were close to the control ones. The production of proinflammatory cytokines in the blood serum was restored to the level of the healthy individuals. Normalization of the number of CD4+-, CD8+-, CD19+-cells was observed in 86.0 ± 3% of the patients. At the same time, against the background of the use of glucosaminylmuramyldipeptide, a more intensive recovery of all links of anti-infectious immunity was recorded in comparison with the group where transfer factor molecules were used. Conclusion: A drug based on glucosaminylmuramyldipeptide can be recommended as the drug of choice in non-specific immunocorrection for complex pharmacotherapy of pyoderma accompanied by secondary immune insufficiency, in comparison with a drug containing transfer factors.

scholarly journals Long-term immunity alterations in the employees of the high hydrogen sulfide content gas condensate processing facility

2021 ◽  
Author(s):  
OV Boiko ◽  
YuI Dotsenko
Keyword(s):  
Hydrogen Sulfide ◽  
Immune System ◽  
Immune Status ◽  
Phagocytic Index ◽  
High Hydrogen ◽  
Gas Processing ◽  
Processing Facility ◽  
Condensate Processing ◽  
Sulfide Content ◽  
Hydrogen Sulfide Content

The current measures for protection of the gas processing plant employees cannot fully prevent the impact of pollutants. Evaluation of the immune system is one of the methods for monitoring of the employees' health, and testing the system of measures used to improve the working conditions. The study was aimed to identify alterations in the immune status of the employees at the gas processing and high hydrogen sulfide content condensate processing facility depending on their working experience. The working environment and the employees' immune system were evaluated by standard methods. Pollutants were detected with the Bruel & Kjaer 1302 Multi-Gas Monitor, and the Tsvet-550 gas chromatographer. A total of 160 employees and 81 controls (blood donors of the regional blood transfusion station) were surveyed. The immune system was evaluated using the System 9000 Plus hematological analyser, Cyto FLEX LX flow cytometer, UNICO 2100UV specrophotometer, and KFK-3-03-ZОМЗ photometer. It was concluded that the existing complex of occupational and industrial hazards affects the immune status of the main production unit employees, which is reflected in the decreased CD20 levels and increased CD8 levels along with the constant levels of CD4. Correlations were revealed between the immuniglobuline level alterations, decrease in the phagocytic index and phagocytic number, as well in lysozyme activity, and the working experience. Pollutant exposure results in altered immunity of the employees, which could be considered the adaptation mechanism.

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