scholarly journals Conservation of Hibiscus acetosella germplasm by seed cryopreservation

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 372-379
Author(s):  
Rodrigo Miranda Moraes ◽  
Fernanda Carlota Nery ◽  
Mayara Caroline Carvalho Pinto ◽  
Renato Paiva ◽  
Sandro Barbosa

Hibiscus acetosella (Malvaceae) is a shrub of great importance for landscaping, food and medicinal purposes. The objective of this study was to preserve H. acetosella germplasm by seed cryopreservation. Half of the seed batch was scarified and the other half was kept intact. Cryopreservation occurred by immersion in liquid nitrogen for 1 hour. Moisture content (MC%), germination percentage (G%), germination speed index (GSI), normal seedling formation (NS%), shoot length (SL), dry matter (DM), biometry and plant survival were evaluated after treatment. MC% ranged between 7.7% and 6.65% in intact and scarified seeds, respectively. Scarification raised G% and GSI compared to intact seeds. Intact and scarified seeds had 100% and 70% NS%, respectively, when not cryopreserved. Cryopreservation reduced NS% to 62% and 12.75%, respectively. The highest SL was observed in intact and non-cryopreserved seeds, with an average of 10.21 cm in height. However, the cryopreservation of intact seeds reduced SL by about 50%, and scarification led to a further reduction, either with (3.32 cm) or without (2.47 cm) cryopreservation. Seedlings from intact and non-cryopreserved seeds showed higher DM in relation to seedlings from cryopreserved seeds. The association of cryopreservation and scarification further reduced DM. The cryopreservation of intact seeds yielded 100% survival at the end of the acclimatization process. However, cryopreservation of scarified seeds reduced the survival percentage to 15%. Changes in color were observed for seeds scarified and subjected to cryopreservation. Thus, cryopreservation is considered an efficient technique for the conservation of intact H. acetosella seeds in the long term.

2019 ◽  
Vol 11 (11) ◽  
pp. 295
Author(s):  
Ricardo A. Felito ◽  
Oscar M. Yamashita ◽  
Wagner Gervazio ◽  
Marco A. C. Carvalho ◽  
Delmonte Roboredo ◽  
...  

The aim of this study was to evaluate the effect of homeopathic medicines in the neutralization of waste herbicide 2,4-D+picloram in cucumber seeds, in the municipality of Alta Floresta-MT process. The experiment was carried out to evaluate two herbicide concentrations and five Nux vomica dynamizations (6CH, 12CH, 18CH, 24CH and 30CH), in addition to two controls, one with distilled water and the other contaminated with herbicide alone. The variables assessed were germination percentage, germination speed index, fresh weight, dry weight, shoot length, root length and stem thickness, where we observed a significant difference in most of the variables analyzed. From the results, it can be concluded that the homeopathic preparations in five dynamics of Nux vomica (6CH, 12CH, 18CH, 24CH and 30CH), act positively on vigor and development of cucumber seeds.


Author(s):  
Maria Silvana Nunes ◽  
Hilderlande Florêncio Da Silva ◽  
Edcarlos Camilo Da Silva ◽  
Luciana Cordeiro Do Nascimento

<p><span class="fontstyle0">The objective of this work was to evaluate the efficiency of essential oils in sanitary and physiological quality in seeds of Enterolobium contortisiliquum. The experiment was carried out at Universidade Federal da Paraíba. The monkfish seeds were manually benefited and scarified to overcome dormancy. Ten treatments were used: control, dicarboximide fungicide and essential oils of Andiroba, eucalyptus, camphor, mint, fennel, basil, rosemary and Copaiba. A health test was performed, with incubation in filter paper, using as a criterion to identify the comparison of characteristics described in the specific literature. The germination test was evaluated up to the fourteenth day, and the variables analyzed were: germination percentage, first count, germination speed index, dead seeds, aerial part length, root and seedling, and fresh and dry matter of seedling. The experimental design used was DIC and the averages were compared using the Scott-Knott test. The essential oils of mint and fennel showed an efficiency equivalent to the conventional method with the use of fungicide and did not cause physiological damage. The essential oil of copaiba should not be used in the treatment of E. Contortisiliquum seeds because they are fungal stimulants and may cause damage to the physiological quality.</span><br /><br /></p>


1970 ◽  
Vol 44 (3) ◽  
pp. 303-310
Author(s):  
A Khatun ◽  
MAH Bhuiyan ◽  
MM Ud-deen

Laboratory experiments were carried out to determine the effects of harvesting stages on the seed quality of chickpea. Chickpea seed of three varieties (BARI Chola-5, BARI Chola-6 and BARI Chola-8) were collected at three harvestings times i.e. from three different times at 7 days interval prior to harvesting, viz. i) when the pods were yellowish with a few yellow greens (H1 stage), ii) when most of the pods were light brown with a few yellow (H2 stage), and iii) when all the pods were completely brown and dry (H3 stage). Significant variation was observed in three varieties of chickpea for all the parameters studied except vigour-I. The highest germination percentage, root length, shoot length, root plus shoot length and vigour-II was observed in BARI Chola-5 which was significantly higher over BARI Chola-8. Dry weight was found to be significantly higher in BARI Chola-8 which was identical to BARI Chola-6. Moisture content was significantly higher in BARI Chola-8. Seed collected at the stage when most of the pods were light brown with a few yellow (H2 stage) recorded the highest germination percentage, dry weight, vigour-I and root length. Pods (H1 stage) gave the highest moisture content in seeds. BARI Chola-5 seeds recorded the highest germination at H2 stage. Interaction effects of varieties and harvesting stage had non-significant effect on germination percentage, vigour-I, shoot length and vigour-II in both the years. Keywords: Chickpea, Harvesting stage, Storage, Vigour, Moisture, Germination DOI: 10.3329/bjsir.v44i3.4403 Bangladesh J. Sci. Ind. Res. 44(3),303-310, 2009  


2018 ◽  
Vol 40 (2) ◽  
pp. 179-184
Author(s):  
Julia Abati ◽  
Cristian Rafael Brzezinski ◽  
Claudemir Zucareli ◽  
Denis Santiago Costa ◽  
Ademir Assis Henning ◽  
...  

Abstract: In soybean crops, industrial seed treatment is widely adopted, and it has allowed the development of new technologies, such as the use of dry powders. This work aimed to evaluate the effect of the industrial treatment, with and without the additional application of dry powder, on the physiological potential of soybean seeds. The experimental design was completely randomized, in a 4×2 factorial scheme, with four replications. Four chemical treatments were evaluated: 1) control, 2) carbendazim + thiram + thiamethoxam, 3) carbendazim + thiram, 4) thiamethoxam. Each trial was divided into one group that was treated and the other that remained untreated with the dry powder. The cultivars BRS 360 RR and BRS 284 were used and analyzed separately. The physiological potential of the seeds was determined by the following assessments: germination, first germination count, seedling emergence in sand, seedling total length, shoot length, and root length. Soybean cultivars respond differently to seed chemical treatments, regarding the effects on physiological potential. Using the dry powder results in problems in seed germination speed in the cultivar BRS 284. In the cultivar BRS 360 RR, the use of dry powder associated with thiamethoxam treatment reduces the physiological potential of the seeds.


1954 ◽  
Vol 5 (3) ◽  
pp. 356 ◽  
Author(s):  
WM Hutton ◽  
JW Peak

Induced autotetraploidy in the Dwalganup variety of subterranean clover (Trifolium subterraneum L.) resulted in total dry weight increases of 60 and 65.5 per cent. at flowering and maturity respectively. In the other four varieties the tetraploids had decreased yields of dry matter compared with the diploids, although the decreases for leaf weights at flowering were nonsignificant in Mount Barker and Tallarook, as was the total dry weight reduction in Tallarook at maturity. There were no significant differences between the diploids and tetraploids in percentage moisture content. When early development was stimulated by growth in a glass-house, the tetraploids of all varieties showed a significant increase in yield of green matter. The level of increased growth was maintained only in Dwalganup, and decreased in other varieties during flowering. An analysis was made of the way in which the different plant parts mere changed by tetraploidy. Where decreased growth occurred, the leaves and stems were coarser. In all varieties a reduced seed-setting followed autotetraploidy, although in Dwalganup the yield of seed per plant was not affected.


2016 ◽  
Vol 50 (6) ◽  
Author(s):  
Babariya C. A. ◽  
Patel J. B. ◽  
Ribadiya Ribadiya ◽  
K. H. Sondarva Jyoti ◽  
Bhatiya V. J.

The present investigation was carried out in laboratory of the Department of Seed Science and Technology, College of Agriculture, Junagadh Agricultural University, Junagadh from August, 2013 to June, 2015, wherein two kg of freshly harvested quality seed of mungbean cv. GM 4 having high germination percentage and low moisture content (below 8%) was taken for each repetition and given the treatments. The experiment was carried out using completely randomized design (CRD). After proper mixing or smearing, seeds were packed in 500 gauge polythene bags and kept in laboratory under ambient condition. Observations were recorded at bimonthly interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results indicated that all the treatments exhibited significant difference for all the traits studied except shoot length after two years of storage. The Indian Minimum Seed Certification Standards in mungbean for germination percentage is 75 per cent. Looking to the germination percentage and Seed vigourindex I and II, among all the treatments, T1 (Neem Seed kernel powder @ 5 g/kg seed), T2 (Neem seed kernel powder @ 10 g/kg seed), T3 (Neem Cake @ 5g/kg Seed) and T4 (Neem Cake @ 10g/kg Seed) recorded the higher values for these traits and can be recommended as a seed treatment before storage for maintaining the seed germination and seedling vigour for more than two years.


1995 ◽  
Vol 124 (3) ◽  
pp. 389-402 ◽  
Author(s):  
M. J. Jones ◽  
M. Singh

SUMMARYTrials were established at two sites in 1982 to compare six 2-course rotations, in which barley alternated with feed legumes (pure or mixed with barley), fallow or barley, in partial factorial combination with different N and P fertilizer regimes. This paper summarizes data from the second to the seventh cropping seasons.Barley yielded most after fallow and more after legumes than after barley; but, on the basis of total dry matter production over both courses, barley-legume rotations outyielded barley-fallow and barley-barley rotations. In terms of net nitrogen offtake, which may be taken as a rough measure of feed value, barley-legumes surpassed the other rotations by c. 100% at both sites, whether the comparison was made on values obtained from plots with or without added fertilizer. Of the legumes, Lathyrus sativus was slightly more productive than Vicia saliva, and this carried over into the rotational yield of both dry matter and nitrogen. Rotations involving pure legumes were slightly more productive than those involving legume/barley mixtures.Yields of all crops and rotations varied greatly from year to year depending on rainfall. Those of the barley-fallow rotation were much less variable than those of the barley-barley rotation at the wetter site (323±103 mm), with the barley-legume rotation intermediate; but variability was much greater at the drier site (250±79 mm), with much less difference between rotations. Nevertheless, fertilizer use and rotations including legumes gave a significant yield advantage under the driest conditions.


Author(s):  
J. J. Gairhe ◽  
T. N. Bhusal ◽  
H. Neupane

 An experiment was conducted during winter, 2010/11 at IAAS, Lamjung with the objective of studying effect of seed priming and nitrogen levels on yield and yield attributing characters of buckwheat. Altogether, there were 9 treatments of seed priming hours (0, 18 and 36) combined with nitrogen levels (30, 45 and 60 kg/ha). The experiment was laid out in randomized complete block design and each treatment was replicated thrice. The seed priming of 36 hours had resulted in significantly higher germination percentage in both 15 days after sowing (DAS) (4.40%) and 30 (DAS) (5.74%) The total germination percentage was also significantly higher (48.4%) with 36 hr seed priming. The main and interaction effects of seed priming and nitrogen levels had significant influence on days to 50% flowering. The dry biomass per plant, grain yield, straw yield, harvest index and percentage moisture content of biomass at harvest were found non-significantly different due to seed priming and nitrogen levels. The highest value for dry matter accumulation per plant (5.24 gm/plant), straw yield (8706 kg/ha) and % moisture content (71.0%) was recorded with 36 hr seed priming whereas the highest value for grain yield (1599 kg/ha), and harvest index (19.8) was obtained with 18 hr seed priming. Likewise, dry matter accumulation (5.38 gm/plant), grain yield (1575 kg/ha) and harvest index (22.0) was found highest in 45 kg N/ha. Similarly, the straw yield (9153 kg/ha) and % moisture content (75.3%) was found highest in the treatment with 60 kg N/ha. The interaction effect between nitrogen level and priming was found non-significant and the highest grain yield (2091 kg/ha) was obtained from 18 hr seed priming with 45 kg N/ha. Phosphorus consumption rate was intrestingly higher in buckwheat plot with nitrogen application. Moderate consumption of potassium was also observed while nitrogen content in soil remain apparantly unchanged and similar was the case with soil pH and organic matter content. Application of phosphatic fertilizer would be benefitial for buckwheat cultivation. Further experiment should be done to verify the effective dose of fertilizer.Journal of the Institute of Agriculture and Animal Science. Vol. 33-34, 2015, Page: 47-54


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 490B-490
Author(s):  
Susan Switras ◽  
Jeffrey Gillman

Syringa vulgaris L. `Montaigne' (French hybrid lilac) and Chionanthus virginicus L. (white fringetree) stock plants were sprayed with two film-forming antitranspirants, Clear Spray and sodium silicate, and one stomate-regulating antitranspirant, atrazine. After 30 days of growth, cuttings were taken from both species. Shoot calipers of antitranspirant-treated lilacs increased at a slower rate than the control lilacs. There were no long-term shoot length changes in lilac due to antitranspirant treatments. Shoots of Clear Spray-treated fringetrees increased in length significantly less than other treatments. Rooting was evaluated once a week from week 3 until 6 weeks after cuttings were taken for lilac and every 2 weeks from week 6 to 12 weeks after sticking for fringetree. All treatments resulted in 80% to 85% rooting in lilac after 6 weeks, but Clear Spray-treated plants rooted more quickly. Fringetree cuttings treated with Clear Spray had a significantly lower rooting percentage (15%) than the other treatments (35% to 50%).


Botany ◽  
2021 ◽  
Author(s):  
Jiajin Li ◽  
Ganesh K. Jaganathan ◽  
Han Kang ◽  
Baolin Liu

In many -but not all- recalcitrant Quercus seeds, water loss during drying only occurs through the scar. Consequently, the embryo axis sitting on the opposite side of the scar is well protected from desiccation. However, whether such a mechanism is common throughout Fagaceae species is unexplored. Similarly, little is known about the desiccation response of other Fagaceae genera. Germination and desiccation tolerance of Castanopsis sclerophylla (Fagaceae) were studied by drying the seeds with silica gel. Fresh seeds had a moisture content (MC) of 36% and germinated to 92.5% when incubated at 15/20°C. Drying seeds to 22.5 and 20.7% MC decreased germination to 27 and 5%, respectively; indicating that seeds are recalcitrant. X-ray computed tomography and photomicrographs showed that the embryo axis of C. sclerophylla is located just below the scar. Regardless of drying occurring either through only scar or the whole pericarp, embryo drying was deemed unavoidable. Nevertheless, germination percentage during drying showed exceptional similarities with the other Fagaceae species.


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