scholarly journals New intravital method for hemolymph collection from Cornu aspersum snails and the establishment of standards for selected biochemical parameters of their hemolymph

2017 ◽  
Vol 73 (6) ◽  
pp. 366-369
Author(s):  
Jerzy Ziętek ◽  
Leszek Guz ◽  
Kinga Panasiuk ◽  
Stanisław Winiarczyk ◽  
Łukasz Adaszek
Keyword(s):  
Health Status ◽  
Biochemical Parameters ◽  
Colorimetric Method ◽  
Alanine Transaminase ◽  
Snail Species ◽  
Aspartate Transaminase ◽  
Research Material ◽  
Main Vessel ◽  
Shell Fragment ◽  
Cornu Aspersum

The aim of the study was to develop an intravital method of collecting hemolymph and to establish standards for the biochemical parameters of the hemolymph of the Cornu aspersum snail species. The research material consisted of 80 Cornu aspersum maxima snails. Hemolymph was collected from the main vessel with a sterile syringe and needle after removing a fragment of the shell. Hemolymph was studied biochemically by the colorimetric method. The parameters evaluated were aspartate transaminase (AST), alanine transaminase (ALT), amylase (AMYL), urea (UREA), and triglycerides (TG). An average of 0.54 ml hemolymph was collected from the animals tested. After 24 hours, a film formed in place of the removed shell fragment and it underwent mineralization within a few days. All animals included in the study survived, and from some of them it was possible to collect hemolymph several times at intervals of several days. The proposed method of hemolymph collection made it possible to obtain material to develop standards for the biochemical parameters of hemolymph and consequently to monitor the health status of these mollusks.

scholarly journals Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae)

2009 ◽  
Vol 52 (2) ◽  
pp. 407-412 ◽  
Author(s):  
Juliana Castro Monteiro ◽  
Sérgio Luis Pinto da Matta ◽  
Fabrícia de Souza Predes ◽  
Tânia Toledo de Oliveira
Keyword(s):  
Plasma Levels ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Alanine Transaminase ◽  
Histopathological Analysis ◽  
Aspartate Transaminase ◽  
Total Proteins ◽  
Daily Dose ◽  
Liver Morphology ◽  
Morphology And Morphometry

Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT), alanine transaminase (ALT), aspartate transaminase (AST), chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG) occurred in the group that received the higher dose.

scholarly journals Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1978
Author(s):  
Davi Vieira Teixeira da Silva ◽  
Aline D’Avila Pereira ◽  
Gilson Teles Boaventura ◽  
Roberto Stefan de Almeida Ribeiro ◽  
Maurício Afonso Verícimo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
Insulin Resistance ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Alanine Transaminase ◽  
Antioxidant Enzyme Activities ◽  
Aspartate Transaminase ◽  
Common Condition ◽  
Short Term

Oxidative stress is a common condition described in risk factors for cardiovascular disease. Betanin, a bioactive pigment from red beetroot demonstrates anti-inflammatory and antioxidant properties. The main aim of this study was to evaluate the short-term intake of betanin against oxidative stress in a rodent model, a common condition described in several risk factors for cardiovascular disease. Oxidative stress was induced in Wistar rats by a hyperlipidemic diet for 60 days, followed by betanin administration (20 mg·kg−1) through oral gavage for 20 days. Plasma biochemical parameters and antioxidant enzyme activities were evaluated. Lipid peroxidation and histopathological changes were determined in the liver. The hyperlipidemic diet caused hyperglycemia, hyperinsulinemia, insulin resistance, and increases in alanine transaminase and aspartate transaminase levels. Oxidative stress status was confirmed by reduction of antioxidant enzyme activities, increased lipid peroxidation, and liver damage. Purified betanin regulated glucose levels, insulin, and insulin resistance. Hepatic damage was reversed as evidenced by the reduction in alanine transaminase and aspartate transaminase levels and confirmed by histological analyses. Betanin reduced hepatic malondialdehyde and increased superoxide dismutase, catalase, and glutathione peroxidase activities. Short-term betanin intake modulated biochemical parameters, reversed hepatic tissue damage, and attenuated oxidative stress in Wistar rats.

Assessment of some hematological indicators of breeding falls of the zabaikalsky breed

2021 ◽  
Vol 1 (2) ◽  
pp. 36-42
Author(s):  
B. Z. Bazaron ◽  
◽  
T. N. Khamiruev ◽  
S. M. Dashinimaev ◽  
G. M. Shkuratova ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Steppe Zone ◽  
Diagnostic Methods ◽  
Clinical State ◽  
Forest Steppe ◽  
Research Material ◽  
Hematological Indicators ◽  
Young Horses

The results of studying the morphological and biochemical parameters of the blood of young horses of the Trans-Baikal breed, depending on the zone of their breeding and age, are presented. The study of hematological parameters is one of the most important diagnostic methods for assessing the physiological and clinical state of animals. The research material was stallions at the age of 12, 18, 24 and 36 months. It was found that hematological parameters were within physiological norms, while in stallions of the forest-steppe zone they were higher in comparison with analogs of the steppe zone. With age, there is a decrease in the estimated hematological parameters in the experimental stallions.

scholarly journals An Ecological Study of the Brown Garden snail Cornu aspersum (Müller, 1774) from selected habitats in Jadiriya in Baghdad vicinity.

2010 ◽  
Vol 7 (1) ◽  
pp. 223-232
Author(s):  
Baghdad Science Journal
Keyword(s):  
Land Snails ◽  
Parasitic Nematodes ◽  
Ecological Indicator ◽  
Snail Species ◽  
The World ◽  
Cornu Aspersum ◽  
Healing Wounds ◽  
Medical Importance ◽  
The One ◽  
Scientific Attention

The present study is considered a pioneer investigation that deal with the terrestrial brown garden snail Cornu aspersum (Müller), in Iraq. Cornu aspersum however is considered an exotic species in many parts of the world ,The species is most probably infiltrates with plants transferred from one place to another. The species has gained importance in many ways. Nutritionally, the species is consumed as food item in many countries, but in other cases it is considered as pest for the damage it causes when feeding on valuable plant shoots. It also has medical importance for its role and ability in healing wounds, burns and remedy of other skin problems. This snail species however may act as a vector for some parasitic nematodes that cause serious diseases. The species is also important in scientific research, usually referred to as ‘ecological indicator’ employed in environmental pollution problems such as the one caused by heavy metals. A great deal of research on this species has been carried out in different parts of the world, however, in Iraq, the scientific attention given to land snails in general and to Cornu aspersum in particular is rare and may be negligible in comparison with the research carried out on aquatic Mollusca species. A total of 1880 individuals of Cornu aspersum were collected from all sampling sites. The study was carried out for the period November 2007 to November 2008 on three sites within the Jadiriya district in Baghdad.. Growth, size classes and shell dimensions of Cornu aspersum populations, and their relationship with major environmental factors; ambient temperature; soil pH, moisture, texture; and the soil content of organic matter have been also investigated. Identification of the species was confirmed by the British Natural History Museum.The data were analyzed by means of standard statistical procedures.

scholarly journals Effects of Cimetidine and Phenobarbitone on Paracetamol Induced Hepatotoxic Rats

1970 ◽  
pp. 13-15
Author(s):  
Layla Afroza Banu ◽  
Hosne Ara Begum ◽  
SAR Choudhury
Keyword(s):  
Enzyme System ◽  
Treated Group ◽  
Protective Role ◽  
Significant Rise ◽  
Alanine Transaminase ◽  
Aspartate Transaminase ◽  
Simultaneous Administration ◽  
Microsomal Enzyme ◽  
Hepatic Microsomal Enzyme ◽  
Long Evans

The effects of cimetidine and phenobarbitone on paracetamol induced hepatotoxicity were studied in Long Evans Norwegian strain rats of either sexes. Orally administration of paracetamol 150 mg/ kg body weight for 21 days. On 22nd days after treatment there was significant increase of serum Alanine transaminase (AST), Aspartate transaminase (AST) and Alkaline phosphatase (Alk. phos) level. Orally administration of phenobarbitone 20 mg/kg b.w. along with paracetamol produced highly significant rise of serum ALT, AST and Alk. phos, levels as compared to the paracetamol treated group. But simultaneous administration of paracetamol and cimetidine produced significant decrease of serum ALT, AST and Alk. phos.level. When phenobarbitone is used concurrently with paracetamol, induced hepatic microsomal enzyme system which in turn aggravates the paracetamol induced hepatotoxicity but when cimetidine was administered simultaneously with paracetamol inhibited hepatic microsomal enzyme system and exhibits a protective role on paracetamol induced hepatotoxicity.The experiment was designed to demonstrate the effect of paracetamol on hepatotoxicity and its prevention by simultaneous administration of cimetidine. Further experiment was also designed to demonstrate the induction of hepatic microsomal enzyme system (HMES) by phenobarbitone on paracetamol induced hepatotoxicity. DOI: 10.3329/bjpp.v23i1.5725Bangladesh J Physiol Pharmacol 2007; 23(1&2) : 13-15

scholarly journals De Ritis Ratio (Aspartate Transaminase/Alanine Transaminase) as a Significant Prognostic Factor in Patients Undergoing Radical Cystectomy with Bladder Urothelial Carcinoma: A Propensity Score-Matched Study

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Hyeong Dong Yuk ◽  
Chang Wook Jeong ◽  
Cheol Kwak ◽  
Hyeon Hoe Kim ◽  
Ja Hyeon Ku
Keyword(s):  
Prognostic Factor ◽  
Propensity Score ◽  
Radical Cystectomy ◽  
Cox Regression ◽  
Alanine Transaminase ◽  
Significant Prognostic Factor ◽  
Aspartate Transaminase ◽  
Cancer Specific Survival ◽  
T Stage ◽  
Kaplan Meier

Introduction. To investigate the correlation between preoperative De Ritis ratio (aspartate transaminase (AST)/alanine transaminase (ALT)) and postoperative outcome in patients with urothelial cell carcinoma (UC) treated with radical cystectomy. Materials and Methods. We analyzed the clinical and pathological data of 771 patients who underwent radical cystectomy for bladder UC. Patients were divided into two groups according to the optimal value of AST/ALT ratio. The effect of the AST/ALT ratio was analyzed using the Kaplan–Meier method and Cox regression hazard models for patients’ cancer-specific survival (CSS), overall survival (OS), and recurrence-free survival (RFS). In addition, propensity score matching of 1 : 1 was performed between the two groups. Results. Median follow-up was 84.0 (36–275) months. Mean age was 64.8±10.0 years. According to the receiver operating characteristic (ROC) analysis, the optimal threshold of the AST/ALT ratio was 1.1. In Kaplan–Meier analyses, the high AST/ALT group showed worse outcomes in CSS and OS (all P<0.001). Also, RFS (P=0.001) in the Cox regression models of clinical and pathological parameters was used to predict CSS, OS, and AST/ALT ratio (HR 2.15, 95% CI 1.23-3.73, P=0.007) and pathological T stage (HR 4.80, 95% CI 1.19-19.28, P=0.003). To predict OS and AST/ALT ratio (HR 2.05, 95% CI 1.65–2.56, P<0.001), pathological T stage (HR 2.96, 95% CI 0.57–17.09, P=0.037) and positive lymph node (HR 1.71, 95% CI 1.50–1.91, P=0.021) were determined as independent prognostic factors. Conclusion. Preoperative AST/ALT ratio could be an independent prognostic factor in patients with UC treated with radical cystectomy.

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