scholarly journals Liver morphology and morphometry and plasma biochemical parameters of Wistar rats that received leaf infusion of Rudgea viburnoides Benth. (Rubiaceae)

2009 ◽  
Vol 52 (2) ◽  
pp. 407-412 ◽  
Author(s):  
Juliana Castro Monteiro ◽  
Sérgio Luis Pinto da Matta ◽  
Fabrícia de Souza Predes ◽  
Tânia Toledo de Oliveira
Keyword(s):  
Plasma Levels ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Alanine Transaminase ◽  
Histopathological Analysis ◽  
Aspartate Transaminase ◽  
Total Proteins ◽  
Daily Dose ◽  
Liver Morphology ◽  
Morphology And Morphometry

Rudgea viburnoides leaves are widely used in popular Brazilian medicine as a diuretic, antirheumatic, hypotensive and blood depurative tea. The present study was carried out to investigate the effects of this infusion on the liver and on the plasma biochemical parameters of Wistar rats. Two groups received the R. viburnoides leaf infusion at a daily dose of 10 or 20g dry-leaves/L water, during 40 days. The histopathological analysis did not show degenerated areas or infiltration of leucocytes. Hepatic morphometry showed accumulation of fat in the hepatocytes of the treated groups. There was no significant change in the plasma levels of urea, creatinin, uric acid, direct bilirubin, cholesterol, total proteins, albumin, gamma glutamyl tranferase (gamma-GT), alanine transaminase (ALT), aspartate transaminase (AST), chlorine, phosphate and calcium. A significant reduction in the plasma levels of triacylglycerol (TAG) occurred in the group that received the higher dose.

scholarly journals Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1978
Author(s):  
Davi Vieira Teixeira da Silva ◽  
Aline D’Avila Pereira ◽  
Gilson Teles Boaventura ◽  
Roberto Stefan de Almeida Ribeiro ◽  
Maurício Afonso Verícimo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
Insulin Resistance ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Alanine Transaminase ◽  
Antioxidant Enzyme Activities ◽  
Aspartate Transaminase ◽  
Common Condition ◽  
Short Term

Oxidative stress is a common condition described in risk factors for cardiovascular disease. Betanin, a bioactive pigment from red beetroot demonstrates anti-inflammatory and antioxidant properties. The main aim of this study was to evaluate the short-term intake of betanin against oxidative stress in a rodent model, a common condition described in several risk factors for cardiovascular disease. Oxidative stress was induced in Wistar rats by a hyperlipidemic diet for 60 days, followed by betanin administration (20 mg·kg−1) through oral gavage for 20 days. Plasma biochemical parameters and antioxidant enzyme activities were evaluated. Lipid peroxidation and histopathological changes were determined in the liver. The hyperlipidemic diet caused hyperglycemia, hyperinsulinemia, insulin resistance, and increases in alanine transaminase and aspartate transaminase levels. Oxidative stress status was confirmed by reduction of antioxidant enzyme activities, increased lipid peroxidation, and liver damage. Purified betanin regulated glucose levels, insulin, and insulin resistance. Hepatic damage was reversed as evidenced by the reduction in alanine transaminase and aspartate transaminase levels and confirmed by histological analyses. Betanin reduced hepatic malondialdehyde and increased superoxide dismutase, catalase, and glutathione peroxidase activities. Short-term betanin intake modulated biochemical parameters, reversed hepatic tissue damage, and attenuated oxidative stress in Wistar rats.

scholarly journals Impact of Magnesium Supplementation in Muscle Damage of Professional Cyclists Competing in a Stage Race

Nutrients ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1927 ◽  
Author(s):  
Alfredo Córdova ◽  
Juan Mielgo-Ayuso ◽  
Enrique Roche ◽  
Alberto Caballero-García ◽  
Diego Fernandez-Lázaro
Keyword(s):  
Muscle Damage ◽  
Alanine Transaminase ◽  
Control Group ◽  
Enzymatic Reactions ◽  
Aspartate Transaminase ◽  
Total Proteins ◽  
Magnesium Supplementation ◽  
Blood Samples ◽  
World Anti Doping Agency ◽  
Last Stage

Magnesium is a cofactor of different enzymatic reactions involved in anabolic and catabolic processes that affect muscular performance during exercise. In addition, it has been suggested that magnesium could participate in maintaining muscle integrity during demanding effort. The main purpose of this study was to analyze the effects of magnesium supplementation in preventing muscle damage in professional cyclists taking part in a 21-day cycling stage race. Eighteen male professional cyclists (n = 18) from two teams were recruited to participate in the research. They were divided into 2 groups: the control group (n = 9) and the magnesium-supplemented group (n = 9). The supplementation consisted of an intake of 400 mg/day of magnesium during the 3 weeks of competition. Blood samples were collected according to World Anti-Doping Agency rules at three specific moments during competition: immediately before the race; mid competition; and before the last stage. Levels of serum and erythrocyte magnesium, lactate dehydrogenase, creatinine kinase, aspartate transaminase, alanine transaminase, myoglobin, aldolase, total proteins, cortisol and creatinine were determined. Serum and erythrocyte magnesium levels decreased during the race. Circulating tissue markers increased at the end of the race in both groups. However, myoglobin increase was mitigated in the supplemented group compared with the controls. We conclude that magnesium supplementation seems to exert a protective effect on muscle damage.

scholarly journals CARDIOPROTECTIVE EFFECT OF PHYLLANTHUS FRATERNUS LEAVES EXTRACT AGAINST CYCLOPHOSPHAMIDE - INDUCED MYOCARDIAL INJURY IN RATS

2017 ◽  
Vol 10 (12) ◽  
pp. 280
Author(s):  
Rakesh Singh Moirangthem ◽  
Ngangom Gunindro ◽  
Dipdeba Singh Takhellambam ◽  
Sucheta Devi Khuraijam ◽  
Meena N ◽  
...  
Keyword(s):  
Protective Effect ◽  
Wistar Rats ◽  
Myocardial Injury ◽  
Histopathological Examination ◽  
Protective Role ◽  
Alanine Transaminase ◽  
Aspartate Transaminase ◽  
Cardiac Biomarker ◽  
Phyllanthus Fraternus

Objectives: This study was undertaken to investigate the possible protective effect of aqueous extract of Phyllanthus fraternus (AEPF) leaves against cyclophosphamide (CP) induced myocardial toxicity in rats.Methods: Wistar rats were given CP single intraperitoneally injection (200 mg/kg) on day 1 of the experiment and two doses of AEPF (200 mg/kg and 400 mg/kg) p.o. daily for 10 days. Cardiac biomarker enzymes such as creatinine kinase (CK), CK isoenzyme MB, lactate dehydrogenase, alkaline phosphatase, alanine transaminase, and aspartate transaminase were determined. Histopathological examinations of the hearts were done.Results: CP treated groups exhibited significantly increased in cardiac biomarker enzymes. Treatment with AEPF prevented the elevation of these enzymes. Potential protective effect was also seen in histopathological examination of the heart characterized by decreased myocardium cell damages in AEPF treatment group.Conclusion: The study showed the protective role of AEPF against CP-induced myocardial injury. The possible role of antioxidant activity is anticipated.

scholarly journals EFFECTS OF TYPE 2 DIABETES MELLITUS INDUCTION AND OF MODERATE PHYSICAL EXERCISE ON THE HEPATIC METABOLISM AND OXIDATIVE STRESS IN WISTAR RATS

2020 ◽  
Vol 20 (39) ◽  
pp. 55-61
Author(s):  
Denise Silva de Oliveira ◽  
Júlia Matzenbacher dos Santos ◽  
Kayorrany Gomes Galvão do Prado ◽  
Andreia Vitor Couto Amaral ◽  
Sandra Aparecida Benite-Ribeiro
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Physical Exercise ◽  
Wistar Rats ◽  
Alanine Transaminase ◽  
The Other ◽  
Low Density ◽  
Aspartate Transaminase

AIMS: The aim of the study was to verify if the induction of Type 2 Diabetes Mellitus (T2DM) by the association of streptozotocin and high fat diet causes hepatic alterations and if physical exercise can improve these alterations.  METHODS: Forty-six Wistar rats were divided into two groups. One group received a diet containing 60% of fat for 9 weeks and on the 14th day they were injected with a single dose of streptozotocin (25mg/kg). The other group, named control group, received a standard diet and a single dose of citrate buffer was given. Animals were them divided into two subgroups: one sedentary (S) and the other submitted to physical exercise (PE), totalizing four groups: C-S; C-PE; HF-S; HF-PE. After euthanasia blood and liver were collected. Plasma and hepatic concentrations of triacylglycerol and very low density lipoprotein; enzyme alanine transaminase, aspartate transaminase and arginase; urea, lactate and 8-isoprostane (an oxidative stress marker) were dosed. RESULTS: Capillary glucose was significantly higher in HF-S and HF-PE group animals compared to C-S and C-PE groups. There were no significant differences in alanine transaminase, arginase, oxidative stress and lactate concentration among the groups. Plasma urea concentration and hepatic aspartate transaminase concentration was lower in HF-S and HF-PE than in C-PE. Concerning liver triacylglycerol and very low density lipoproteins, both were higher in the HF-PE group when compared to the C-S. CONCLUSIONS: We conclude that diet was decisive for the aforementioned parameters alterations and that physical exercise protocol was not efficient to improve analyzed parameters.

scholarly journals Endothelial, renal and hepatic variables in wistar rats treated with Vancomycin

2014 ◽  
Vol 86 (4) ◽  
pp. 1963-1972 ◽  
Author(s):  
FELIPE R. BRUNIERA ◽  
FELIPE M. FERREIRA ◽  
LUIZ R.M. SAVIOLI ◽  
MARCELO R. BACCI ◽  
DAVID FEDER ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Wistar Rats ◽  
Femoral Vein ◽  
Beta Carotene ◽  
Alpha Tocopherol ◽  
Histopathological Analysis ◽  
First Choice ◽  
Liver And Kidney ◽  
Hs Crp ◽  
Kidney Liver

Vancomycin (VCM) is indicated in combat against Gram-positive infections, but it is not considered a first-choice drug because of its adverse effects. It is believed that oxidative stress is the primary mechanism of endothelial injury and the consequent VCM toxicity, which varies from phlebitis to nephrotoxicity. Moreover, dose recommendations, dilution, rates and types of infusion are still controversial. The aim of this study was to determine the effect of different VCM dilutions in endothelial, liver and kidney injuries by biochemical parameters and histopathological analysis. Wistar rats were randomly divided into six groups and subjected to femoral vein cannulation for drug administration. Control groups received 0.9 ml of saline and the others received VCM (10mg/Kg/day) at dilutions of 5.0 and 10.0 mg/mL for 3 and 7 days. Homocysteine, hs-CRP, AST, ALT, GGT, urea, creatinine, lycopene, alpha-tocopherol, beta-carotene and retinol were analyzed. Kidney, liver and cannulated femoral vein fragments were collected.This study showed alterations in ALT which featured hepatotoxicity. However, drug dilutions were not able to show changes in other biochemical parameters. In contrast, kidney and endothelium pathological changes were observed. More studies are needed to characterize VCM induced kidney and endothelium toxicity and biochemical markers able to show such morphological modifications.

scholarly journals Preventive Effect of Ganoderma lucidum on Paracetamol-induced Acute Hepatotoxicity in Rats

2013 ◽  
Vol 5 (3) ◽  
pp. 573-578 ◽  
Author(s):  
M. M. Rahman ◽  
S. Hossain
Keyword(s):  
Oral Administration ◽  
Plasma Levels ◽  
Ganoderma Lucidum ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Hepatoprotective Activity ◽  
Total Plasma ◽  
Preventive Effect ◽  
Aspartate Amino Transferase

The effect of Ganoderma lucidum in paracetamol-induced acute hepatotoxicity was investigated on Wistar rats in the present study. Hepatotoxicity was induced by oral administration of paracetamol (500 mg/kg of body weight) for 7 consecutive days. The extent of liver damage was studied by assessing biochemical parameters comprising of plasma aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), total bilirubin (TB) and total plasma total protein (TP). Oral administration of paracetamol significantly increased the plasma levels of AST, ALT, ALP and LDH with concomitant decrease of TP. The feeding of G. lucidum (1% powder with basal food) significantly reversed the plasma levels of ALT, AST, ALP, LDH, and TP nearly to those of the normal rats. Thus, the results of the present demonstrate that the G. lucidum has significant hepatoprotective activity against paracetamol induced acute hepatotoxicity in rats.

scholarly journals New intravital method for hemolymph collection from Cornu aspersum snails and the establishment of standards for selected biochemical parameters of their hemolymph

2017 ◽  
Vol 73 (6) ◽  
pp. 366-369
Author(s):  
Jerzy Ziętek ◽  
Leszek Guz ◽  
Kinga Panasiuk ◽  
Stanisław Winiarczyk ◽  
Łukasz Adaszek
Keyword(s):  
Health Status ◽  
Biochemical Parameters ◽  
Colorimetric Method ◽  
Alanine Transaminase ◽  
Snail Species ◽  
Aspartate Transaminase ◽  
Research Material ◽  
Main Vessel ◽  
Shell Fragment ◽  
Cornu Aspersum

The aim of the study was to develop an intravital method of collecting hemolymph and to establish standards for the biochemical parameters of the hemolymph of the Cornu aspersum snail species. The research material consisted of 80 Cornu aspersum maxima snails. Hemolymph was collected from the main vessel with a sterile syringe and needle after removing a fragment of the shell. Hemolymph was studied biochemically by the colorimetric method. The parameters evaluated were aspartate transaminase (AST), alanine transaminase (ALT), amylase (AMYL), urea (UREA), and triglycerides (TG). An average of 0.54 ml hemolymph was collected from the animals tested. After 24 hours, a film formed in place of the removed shell fragment and it underwent mineralization within a few days. All animals included in the study survived, and from some of them it was possible to collect hemolymph several times at intervals of several days. The proposed method of hemolymph collection made it possible to obtain material to develop standards for the biochemical parameters of hemolymph and consequently to monitor the health status of these mollusks.

Study on Kidney Function in Female Workers Exposed to Perchlorethylene

1990 ◽  
Vol 9 (6) ◽  
pp. 377-380 ◽  
Author(s):  
A. Vyskočil ◽  
S. Emminger ◽  
J. Tejral ◽  
Z. Fiala ◽  
E. Ettlerova ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Biochemical Parameters ◽  
Renal Damage ◽  
Ambient Air ◽  
Exposed Group ◽  
Control Group ◽  
Total Proteins ◽  
Dry Cleaning ◽  
Female Workers ◽  
Occupationally Exposed

1 Biochemical markers of kidney damage were examined in 16 female workers chronically exposed to tetrachlorethylene (TCE) in five dry-cleaning shops. The results were compared with those obtained in 13 females non-occupationally exposed to organic solvents. 2 The intensity of exposure was monitored by personal environmental monitoring. The time-weighed average exposure to TCE amounted to 157 mg m-3 (range 9-799 mg m-3). A satisfactory agreement was found between the concentration of TCE in ambient air sampled with the charcoal tube method and with a passive dosimeter. 3 The urinary excretion of lysozyme was increased in the exposed group. No difference was found in the urinary excretion of albumin, β2-microglobulin, lactate dehydrogenase, total proteins or glucose. The prevalence of abnormal values of biochemical parameters in the exposed group did not differ from that observed in the control group. No correlation was found between the level of TCE exposure and biochemical parameters. 4 The present study suggests that chronic exposure to TCE does not lead to renal damage.

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

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